AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007264

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007264.t1 MTR_7g104050 83.730 252 23 4 1 247 1 239 3.53e-140 398
MS.gene007264.t1 MTR_7g104050 83.730 252 23 4 1 247 1 239 4.14e-140 398
MS.gene007264.t1 MTR_0001s0490 61.404 114 41 3 72 184 24 135 3.09e-40 142
MS.gene007264.t1 MTR_4g125490 50.340 147 51 5 63 202 6 137 5.83e-38 137
MS.gene007264.t1 MTR_8g020560 58.407 113 43 3 72 182 10 120 1.36e-36 132
MS.gene007264.t1 MTR_2g041430 55.556 126 41 4 63 182 4 120 2.33e-36 132
MS.gene007264.t1 MTR_5g027030 62.037 108 29 4 74 181 17 112 2.44e-36 131
MS.gene007264.t1 MTR_2g041430 55.556 126 41 4 63 182 4 120 2.57e-36 132
MS.gene007264.t1 MTR_2g041430 55.556 126 41 4 63 182 4 120 2.80e-36 132
MS.gene007264.t1 MTR_1g017490 54.545 121 43 3 69 181 7 123 7.46e-34 128
MS.gene007264.t1 MTR_1g017490 54.701 117 49 2 69 181 140 256 9.92e-34 128
MS.gene007264.t1 MTR_3g092330 42.222 135 51 3 55 184 94 206 2.72e-27 110
MS.gene007264.t1 MTR_8g035910 39.623 159 61 7 74 221 50 184 8.24e-21 90.9
MS.gene007264.t1 MTR_8g035910 60.784 51 20 0 137 187 297 347 1.34e-14 73.2
MS.gene007264.t1 MTR_8g035640 58.000 50 21 0 137 186 51 100 1.04e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007264.t1 AT2G36400 59.799 199 56 7 1 184 1 190 1.45e-64 206
MS.gene007264.t1 AT3G52910 58.209 201 57 8 1 181 1 194 1.56e-61 198
MS.gene007264.t1 AT4G37740 46.053 152 66 5 69 214 160 301 1.75e-34 130
MS.gene007264.t1 AT3G13960 54.545 110 46 3 74 181 17 124 3.37e-31 119
MS.gene007264.t1 AT2G22840 55.556 117 42 4 69 181 129 239 1.18e-29 116
MS.gene007264.t1 AT4G24150 39.535 172 86 4 71 224 109 280 3.29e-26 106
MS.gene007264.t1 AT4G24150 39.535 172 86 4 71 224 147 318 4.89e-26 105
MS.gene007264.t1 AT2G06200 50.427 117 47 4 74 179 7 123 1.38e-25 101
MS.gene007264.t1 AT2G06200 50.427 117 47 4 74 179 46 162 1.42e-25 102
MS.gene007264.t1 AT5G53660 47.899 119 43 3 74 190 60 161 2.02e-25 103
MS.gene007264.t1 AT4G24150 35.533 197 84 5 71 224 147 343 1.21e-22 96.7
MS.gene007264.t1 AT2G45480 48.039 102 53 0 77 178 28 129 5.66e-22 92.8
MS.gene007264.t1 AT2G45480 48.039 102 53 0 77 178 28 129 2.53e-21 92.4
MS.gene007264.t1 AT2G45480 45.977 87 31 3 120 199 286 363 2.12e-17 81.3
MS.gene007264.t1 AT2G45480 48.039 102 53 0 77 178 26 127 2.66e-21 92.4
MS.gene007264.t1 AT2G45480 45.977 87 31 3 120 199 284 361 2.23e-17 81.3
MS.gene007264.t1 AT2G06200 71.111 45 11 1 137 179 7 51 8.16e-16 73.6

Find 61 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCAAGAACTTGAGTTAC+AGG 0.267624 7.4:-6887981 MS.gene007264:CDS
TGAGCAGGGTGAAGAAGTTT+TGG 0.323925 7.4:+6888287 None:intergenic
TGATAATGATGAAGAAAATA+AGG 0.326826 7.4:+6887872 None:intergenic
TTGATAGGTTGAAGAAGTTC+AGG 0.344449 7.4:+6887917 None:intergenic
ATCCAGAGCCAGGTCGTTGC+CGG 0.359541 7.4:-6887595 MS.gene007264:CDS
CACTTAGCTGAGGTGGCATC+AGG 0.370292 7.4:-6886250 MS.gene007264:intron
TAGTGTTGCAAAGTGGGTAC+TGG 0.393066 7.4:-6887634 MS.gene007264:CDS
CTCCGGCAACGACCTGGCTC+TGG 0.400600 7.4:+6887593 None:intergenic
TGTGCGAGGGATGTGGTGGC+TGG 0.410154 7.4:-6887548 MS.gene007264:CDS
TGTTGGTGGTGTTGATGAGC+AGG 0.411541 7.4:+6888272 None:intergenic
CAAGTTGGTGACTTTGGTGT+TGG 0.425449 7.4:+6888217 None:intergenic
ATCTGACAAGTTGGTGACTT+TGG 0.426402 7.4:+6888211 None:intergenic
CTCCACATGTATCTAAAGTC+TGG 0.428042 7.4:+6886220 None:intergenic
GCCACTGAGATAAGCTGAAA+TGG 0.433868 7.4:+6887999 None:intergenic
GTGACTTTGGTGTTGGGTTG+AGG 0.434579 7.4:+6888224 None:intergenic
CTTACTAGGAAATCTGTTGT+TGG 0.450424 7.4:+6888175 None:intergenic
GGAGCAATGGATCCAGAGCC+AGG 0.451345 7.4:-6887605 MS.gene007264:CDS
GTTTCTCTGACTCATGCTGT+TGG 0.459512 7.4:+6888327 None:intergenic
AAGTTGGTGACTTTGGTGTT+GGG 0.464829 7.4:+6888218 None:intergenic
TACATTATTAGGAATGGGAA+TGG 0.465443 7.4:-6888026 MS.gene007264:intron
CAAAGTGGGTACTGGGGAAG+AGG 0.492409 7.4:-6887626 MS.gene007264:CDS
GTGGCGGTGTGCGAGGGATG+TGG 0.497767 7.4:-6887555 MS.gene007264:CDS
AGTGTTGCAAAGTGGGTACT+GGG 0.501618 7.4:-6887633 MS.gene007264:CDS
GTTGGTGTGGGAAGTTCCAC+AGG 0.502197 7.4:+6887473 None:intergenic
TGATTAAAGTGAAGATCAAA+AGG 0.508625 7.4:+6887365 None:intergenic
GTTCCACAGGCTTTCTTGAA+CGG 0.512655 7.4:+6887486 None:intergenic
TCTGTTCTCCGGCAACGACC+TGG 0.516750 7.4:+6887587 None:intergenic
ACATTATTAGGAATGGGAAT+GGG 0.520045 7.4:-6888025 MS.gene007264:intron
TTTGATATTTAGATACATGT+TGG 0.524543 7.4:-6887957 MS.gene007264:CDS
TTGTTTCGTATGAAAGTCCA+TGG 0.526188 7.4:+6888358 None:intergenic
TTGATCTTCACTTTAATCAA+CGG 0.532715 7.4:-6887361 MS.gene007264:intron
AAGGAAGTGCAGGTGATTGT+TGG 0.537153 7.4:+6888255 None:intergenic
CTTAGCTGAGGTGGCATCAG+GGG 0.547485 7.4:-6886248 MS.gene007264:intron
CTTCCCACACCAACTAGTAA+TGG 0.551902 7.4:-6887464 MS.gene007264:CDS
ATCAAAAGGAGATTTGAATG+AGG 0.554725 7.4:+6887379 None:intergenic
GTTGGTGGTGTTGATGAGCA+GGG 0.556192 7.4:+6888273 None:intergenic
ACCGCCATTACTAGTTGGTG+TGG 0.561256 7.4:+6887460 None:intergenic
ATATTTAGATACATGTTGGT+TGG 0.568638 7.4:-6887953 MS.gene007264:CDS
AAAAGGAGATTTGAATGAGG+AGG 0.578590 7.4:+6887382 None:intergenic
TAATGCTGAATCTGACAAGT+TGG 0.579106 7.4:+6888202 None:intergenic
GTACTGGGGAAGAGGAGCAA+TGG 0.581936 7.4:-6887618 MS.gene007264:CDS
GATCCACCGCCATTACTAGT+TGG 0.583483 7.4:+6887455 None:intergenic
AGCTGAGGTGGCATCAGGGG+CGG 0.586071 7.4:-6886245 MS.gene007264:intron
GGTACAAACAAAGGAAGTGC+AGG 0.589399 7.4:+6888245 None:intergenic
GAAGTGCAGGTGATTGTTGG+TGG 0.603904 7.4:+6888258 None:intergenic
GCCATTTCAGCTTATCTCAG+TGG 0.605314 7.4:-6888000 MS.gene007264:CDS
GCGGTGTGCGAGGGATGTGG+TGG 0.605687 7.4:-6887552 MS.gene007264:CDS
ACTTAGCTGAGGTGGCATCA+GGG 0.606830 7.4:-6886249 MS.gene007264:intron
ACACCAACTAGTAATGGCGG+TGG 0.613692 7.4:-6887458 MS.gene007264:CDS
GGTCGTTGCCGGAGAACAGA+CGG 0.624707 7.4:-6887584 MS.gene007264:CDS
TGGACTTTCATACGAAACAA+TGG 0.628358 7.4:-6888355 MS.gene007264:CDS
ATCCAGACTTTAGATACATG+TGG 0.636745 7.4:-6886222 MS.gene007264:CDS
CCGCCATTACTAGTTGGTGT+GGG 0.647594 7.4:+6887461 None:intergenic
TTGGGTTGAGGTACAAACAA+AGG 0.648493 7.4:+6888236 None:intergenic
AAACCGTTCAAGAAAGCCTG+TGG 0.653157 7.4:-6887489 MS.gene007264:CDS
ATAGGTTGAAGAAGTTCAGG+AGG 0.653453 7.4:+6887920 None:intergenic
GAATGAAACTTACATGCTGT+AGG 0.658238 7.4:+6887848 None:intergenic
CCCACACCAACTAGTAATGG+CGG 0.678913 7.4:-6887461 MS.gene007264:CDS
AATGAGGAGGAAGTAACAAG+AGG 0.678921 7.4:+6887395 None:intergenic
GTGTTGCAAAGTGGGTACTG+GGG 0.700679 7.4:-6887632 MS.gene007264:CDS
TGTGAAAGACACATGCATCG+TGG 0.747726 7.4:-6887515 MS.gene007264:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATCTTTAATAAGAT+TGG - chr7.4:6887600-6887619 MS.gene007264:CDS 10.0%
!! TCTAAATATAGTTAAAAAAT+CGG + chr7.4:6887416-6887435 None:intergenic 10.0%
!! ATTTACTAATTAAATGACAA+TGG - chr7.4:6887228-6887247 MS.gene007264:intron 15.0%
!! ATTTATGTATAATCTACTAA+CGG + chr7.4:6887577-6887596 None:intergenic 15.0%
!! CATAAAGATTAAAAATCATA+TGG + chr7.4:6888026-6888045 None:intergenic 15.0%
!!! AAATATCCATTTTTTTTCTT+AGG + chr7.4:6888195-6888214 None:intergenic 15.0%
!!! AATATCCATTTTTTTTCTTA+GGG + chr7.4:6888194-6888213 None:intergenic 15.0%
!!! AATTTAGTTTTAGAAAGAAA+CGG + chr7.4:6887981-6888000 None:intergenic 15.0%
!!! CATATGATTTTTAATCTTTA+TGG - chr7.4:6888024-6888043 MS.gene007264:intron 15.0%
!! AAGATTAAAAATCATATGGA+CGG + chr7.4:6888022-6888041 None:intergenic 20.0%
!! AAGTAAAAATAATGTTACTG+GGG + chr7.4:6886813-6886832 None:intergenic 20.0%
!! CAAGTAAAAATAATGTTACT+GGG + chr7.4:6886814-6886833 None:intergenic 20.0%
!! TAAATCTTTAATAAGATTGG+TGG - chr7.4:6887603-6887622 MS.gene007264:CDS 20.0%
!! TAACAAGTTCTTACATTATT+AGG - chr7.4:6886494-6886513 MS.gene007264:intron 20.0%
!! TACTAGTAGTAATTATAAGT+GGG - chr7.4:6888097-6888116 MS.gene007264:intron 20.0%
!! TTTAAGCCTTAAAGTTAATT+AGG + chr7.4:6888128-6888147 None:intergenic 20.0%
!! TTTGATATTTAGATACATGT+TGG - chr7.4:6886574-6886593 MS.gene007264:intron 20.0%
!!! AATTCAAACTTACTTTTTGA+AGG + chr7.4:6887797-6887816 None:intergenic 20.0%
!!! ATATCCATTTTTTTTCTTAG+GGG + chr7.4:6888193-6888212 None:intergenic 20.0%
!!! TGATAATGATGAAGAAAATA+AGG + chr7.4:6886662-6886681 None:intergenic 20.0%
!!! TTTTGTCATCTTTTTAAGAT+GGG - chr7.4:6887763-6887782 MS.gene007264:intron 20.0%
! AAAATACTACTAGTAGCTTT+GGG - chr7.4:6887330-6887349 MS.gene007264:intron 25.0%
! AAAATCAGCAAATCAAAAGA+GGG + chr7.4:6888236-6888255 None:intergenic 25.0%
! ACTACAAGTAATTACTCATT+AGG + chr7.4:6887268-6887287 None:intergenic 25.0%
! AGTAAAAATAATGTTACTGG+GGG + chr7.4:6886812-6886831 None:intergenic 25.0%
! AGTTCTTACATTATTAGGAA+TGG - chr7.4:6886499-6886518 MS.gene007264:intron 25.0%
! ATATTTAGATACATGTTGGT+TGG - chr7.4:6886578-6886597 MS.gene007264:intron 25.0%
! ATTATTTGAACCAAATCCAA+AGG + chr7.4:6887952-6887971 None:intergenic 25.0%
! CCAAGTAAAAATAATGTTAC+TGG + chr7.4:6886815-6886834 None:intergenic 25.0%
! CTAACTCAAAATAACTTACT+AGG + chr7.4:6886373-6886392 None:intergenic 25.0%
! GTACTAGTAGTAATTATAAG+TGG - chr7.4:6888096-6888115 MS.gene007264:intron 25.0%
! GTCATACCTAATTAACTTTA+AGG - chr7.4:6888119-6888138 MS.gene007264:intron 25.0%
! GTTCTTACATTATTAGGAAT+GGG - chr7.4:6886500-6886519 MS.gene007264:intron 25.0%
! TAATTACACCTTCCATTATT+TGG + chr7.4:6887830-6887849 None:intergenic 25.0%
! TGATTAAAGTGAAGATCAAA+AGG + chr7.4:6887169-6887188 None:intergenic 25.0%
! TTGATCTTCACTTTAATCAA+CGG - chr7.4:6887170-6887189 MS.gene007264:intron 25.0%
! TTTGATGCATAACCAAATAA+TGG - chr7.4:6887815-6887834 MS.gene007264:intron 25.0%
!! TTTTATTAGTGTTGCAAAGT+GGG - chr7.4:6886891-6886910 MS.gene007264:intron 25.0%
!! TTTTTATTAGTGTTGCAAAG+TGG - chr7.4:6886890-6886909 MS.gene007264:intron 25.0%
!!! AGAAAAGAAAGTGTGTTTTT+TGG + chr7.4:6886757-6886776 None:intergenic 25.0%
!!! AGTTAAAAAATCGGTTTTCA+CGG + chr7.4:6887407-6887426 None:intergenic 25.0%
!!! CCAGTAACATTATTTTTACT+TGG - chr7.4:6886812-6886831 MS.gene007264:intron 25.0%
!!! CTTTTGTCATCTTTTTAAGA+TGG - chr7.4:6887762-6887781 MS.gene007264:intron 25.0%
!!! TATCCATTTTTTTTCTTAGG+GGG + chr7.4:6888192-6888211 None:intergenic 25.0%
!!! TGTGTTTTTTGGTTTTGTTT+TGG + chr7.4:6886746-6886765 None:intergenic 25.0%
AATAATGAAGCCTTTGGATT+TGG - chr7.4:6887939-6887958 MS.gene007264:CDS 30.0%
AATTAGTTCAACATTGTGAC+AGG - chr7.4:6887845-6887864 MS.gene007264:intron 30.0%
AATTTCAAACTTGAACCAAG+TGG + chr7.4:6887551-6887570 None:intergenic 30.0%
ACATTATTAGGAATGGGAAT+GGG - chr7.4:6886506-6886525 MS.gene007264:intron 30.0%
ATACTATTGTAGTACGACAT+TGG + chr7.4:6888073-6888092 None:intergenic 30.0%
ATCAAAAGGAGATTTGAATG+AGG + chr7.4:6887155-6887174 None:intergenic 30.0%
ATGAAGATCATTTAATCCAG+TGG - chr7.4:6887706-6887725 MS.gene007264:intron 30.0%
ATTCTACTTGGTTTCTTACA+CGG - chr7.4:6886702-6886721 MS.gene007264:intron 30.0%
CAAAATACTACTAGTAGCTT+TGG - chr7.4:6887329-6887348 MS.gene007264:intron 30.0%
CAAAATCAGCAAATCAAAAG+AGG + chr7.4:6888237-6888256 None:intergenic 30.0%
CATGTAAGTTTCATTCTACT+TGG - chr7.4:6886690-6886709 MS.gene007264:intron 30.0%
CTAAACAATAGTATGTGCTA+GGG + chr7.4:6887524-6887543 None:intergenic 30.0%
GACAGAAAAAAAAGTTGTTC+TGG + chr7.4:6887743-6887762 None:intergenic 30.0%
TACATTATTAGGAATGGGAA+TGG - chr7.4:6886505-6886524 MS.gene007264:intron 30.0%
TGAAGATCATTTAATCCAGT+GGG - chr7.4:6887707-6887726 MS.gene007264:intron 30.0%
TTGCCAAGTATGAAACATAT+TGG + chr7.4:6887920-6887939 None:intergenic 30.0%
! ACTTTCTTTTCTTCGTGTTT+TGG - chr7.4:6886765-6886784 MS.gene007264:intron 30.0%
! ATCGCAACTATTTGAAACAT+AGG + chr7.4:6888164-6888183 None:intergenic 30.0%
! ATGCATAACCAAATAATGGA+AGG - chr7.4:6887819-6887838 MS.gene007264:intron 30.0%
! CTACTACATCTTTCTTCTTT+TGG - chr7.4:6886460-6886479 MS.gene007264:intron 30.0%
! CTTTCTTTTCTTCGTGTTTT+GGG - chr7.4:6886766-6886785 MS.gene007264:intron 30.0%
! TCGCAACTATTTGAAACATA+GGG + chr7.4:6888163-6888182 None:intergenic 30.0%
! TGAAGAAGACTTTTCTTGAT+AGG + chr7.4:6886632-6886651 None:intergenic 30.0%
!! GTAAGAACTTGTTAAAGTGT+TGG + chr7.4:6886488-6886507 None:intergenic 30.0%
!! GTTAAAAAATCGGTTTTCAC+GGG + chr7.4:6887406-6887425 None:intergenic 30.0%
!!! AGTTTTAGAAAGAAACGGTT+GGG + chr7.4:6887976-6887995 None:intergenic 30.0%
!!! TAGTTTTAGAAAGAAACGGT+TGG + chr7.4:6887977-6887996 None:intergenic 30.0%
!!! TTGTTTTGGAGTATGTGATA+TGG + chr7.4:6886732-6886751 None:intergenic 30.0%
AAAAGGAGATTTGAATGAGG+AGG + chr7.4:6887152-6887171 None:intergenic 35.0%
ACTGATACCAAACTGAGATA+CGG - chr7.4:6887873-6887892 MS.gene007264:CDS 35.0%
ATCCAGACTTTAGATACATG+TGG - chr7.4:6888309-6888328 MS.gene007264:CDS 35.0%
CCTAAACAATAGTATGTGCT+AGG + chr7.4:6887525-6887544 None:intergenic 35.0%
CCTAGCACATACTATTGTTT+AGG - chr7.4:6887522-6887541 MS.gene007264:CDS 35.0%
CTTACTAGGAAATCTGTTGT+TGG + chr7.4:6886359-6886378 None:intergenic 35.0%
GAATGAAACTTACATGCTGT+AGG + chr7.4:6886686-6886705 None:intergenic 35.0%
TAATGCTGAATCTGACAAGT+TGG + chr7.4:6886332-6886351 None:intergenic 35.0%
TGACCCCCTAAGAAAAAAAA+TGG - chr7.4:6888186-6888205 MS.gene007264:CDS 35.0%
TGGACTTTCATACGAAACAA+TGG - chr7.4:6886176-6886195 MS.gene007264:CDS 35.0%
TGGCCAATATGTTTCATACT+TGG - chr7.4:6887914-6887933 MS.gene007264:CDS 35.0%
TTGGCAAATAATGAAGCCTT+TGG - chr7.4:6887933-6887952 MS.gene007264:CDS 35.0%
! CGCAACTATTTGAAACATAG+GGG + chr7.4:6888162-6888181 None:intergenic 35.0%
! TTGATAGGTTGAAGAAGTTC+AGG + chr7.4:6886617-6886636 None:intergenic 35.0%
!! AAGAAGTTTTGGCATCTTTG+TGG + chr7.4:6886236-6886255 None:intergenic 35.0%
!!! GTTTTAGAAAGAAACGGTTG+GGG + chr7.4:6887975-6887994 None:intergenic 35.0%
!!! TATTTTGACCCCCTATGTTT+TGG - chr7.4:6888210-6888229 MS.gene007264:CDS 35.0%
AAAAGAGGGCCAAAACATAG+GGG + chr7.4:6888222-6888241 None:intergenic 40.0%
AATGAGGAGGAAGTAACAAG+AGG + chr7.4:6887139-6887158 None:intergenic 40.0%
CAAAAGAGGGCCAAAACATA+GGG + chr7.4:6888223-6888242 None:intergenic 40.0%
GGTGCAAATAATTGAACCGT+CGG + chr7.4:6887358-6887377 None:intergenic 40.0%
TCAAAAGAGGGCCAAAACAT+AGG + chr7.4:6888224-6888243 None:intergenic 40.0%
! ACTTTTTACCGTCTGTTCTC+CGG + chr7.4:6886958-6886977 None:intergenic 40.0%
! ATAGGTTGAAGAAGTTCAGG+AGG + chr7.4:6886614-6886633 None:intergenic 40.0%
! CTCCACATGTATCTAAAGTC+TGG + chr7.4:6888314-6888333 None:intergenic 40.0%
! TCGGTTTTCACGGGTAAAAA+CGG + chr7.4:6887397-6887416 None:intergenic 40.0%
! TTTTCACGGGTAAAAACGGA+CGG + chr7.4:6887393-6887412 None:intergenic 40.0%
!! AAGTTGGTGACTTTGGTGTT+GGG + chr7.4:6886316-6886335 None:intergenic 40.0%
!! ATCTGACAAGTTGGTGACTT+TGG + chr7.4:6886323-6886342 None:intergenic 40.0%
!! CTATTGTTTAGGTTGCCACT+TGG - chr7.4:6887533-6887552 MS.gene007264:CDS 40.0%
!! TTGGGTTGAGGTACAAACAA+AGG + chr7.4:6886298-6886317 None:intergenic 40.0%
AAACCGTTCAAGAAAGCCTG+TGG - chr7.4:6887042-6887061 MS.gene007264:intron 45.0%
AAAGAGGGCCAAAACATAGG+GGG + chr7.4:6888221-6888240 None:intergenic 45.0%
ACAATAGTATGTGCTAGGGC+TGG + chr7.4:6887520-6887539 None:intergenic 45.0%
ACTTGACACATGGACGTTGA+TGG + chr7.4:6888261-6888280 None:intergenic 45.0%
AGTGTTGCAAAGTGGGTACT+GGG - chr7.4:6886898-6886917 MS.gene007264:intron 45.0%
CAGCTAAGTGACTTGACACA+TGG + chr7.4:6888271-6888290 None:intergenic 45.0%
CGTAGAAACCGAGATGCAAA+CGG + chr7.4:6887461-6887480 None:intergenic 45.0%
CGTTTGCATCTCGGTTTCTA+CGG - chr7.4:6887459-6887478 MS.gene007264:CDS 45.0%
CTTCCCACACCAACTAGTAA+TGG - chr7.4:6887067-6887086 MS.gene007264:intron 45.0%
GAACAGACGGTAAAAAGTGG+CGG - chr7.4:6886960-6886979 MS.gene007264:intron 45.0%
GCCACTGAGATAAGCTGAAA+TGG + chr7.4:6886535-6886554 None:intergenic 45.0%
GCCATTTCAGCTTATCTCAG+TGG - chr7.4:6886531-6886550 MS.gene007264:intron 45.0%
GGAGAACAGACGGTAAAAAG+TGG - chr7.4:6886957-6886976 MS.gene007264:intron 45.0%
GTAGAAACCGAGATGCAAAC+GGG + chr7.4:6887460-6887479 None:intergenic 45.0%
GTGGCAAGAACTTGAGTTAC+AGG - chr7.4:6886550-6886569 MS.gene007264:intron 45.0%
GTTCCACAGGCTTTCTTGAA+CGG + chr7.4:6887048-6887067 None:intergenic 45.0%
GTTTCTCTGACTCATGCTGT+TGG + chr7.4:6886207-6886226 None:intergenic 45.0%
TAGTGTTGCAAAGTGGGTAC+TGG - chr7.4:6886897-6886916 MS.gene007264:intron 45.0%
TGTGAAAGACACATGCATCG+TGG - chr7.4:6887016-6887035 MS.gene007264:intron 45.0%
TGTGTCAAGTCACTTAGCTG+AGG - chr7.4:6888271-6888290 MS.gene007264:CDS 45.0%
TTTAGACCCGTTGACCAAAC+CGG - chr7.4:6887430-6887449 MS.gene007264:CDS 45.0%
! AAGGAAGTGCAGGTGATTGT+TGG + chr7.4:6886279-6886298 None:intergenic 45.0%
! CTCGGTTTCTACGGATTGTT+CGG - chr7.4:6887468-6887487 MS.gene007264:CDS 45.0%
! GGTACAAACAAAGGAAGTGC+AGG + chr7.4:6886289-6886308 None:intergenic 45.0%
! TCTACGGATTGTTCGGTTTC+CGG - chr7.4:6887475-6887494 MS.gene007264:CDS 45.0%
! TGAGCAGGGTGAAGAAGTTT+TGG + chr7.4:6886247-6886266 None:intergenic 45.0%
!! CAAGTTGGTGACTTTGGTGT+TGG + chr7.4:6886317-6886336 None:intergenic 45.0%
ACACCAACTAGTAATGGCGG+TGG - chr7.4:6887073-6887092 MS.gene007264:intron 50.0%
ACTTAGCTGAGGTGGCATCA+GGG - chr7.4:6888282-6888301 MS.gene007264:CDS 50.0%
ATTGGCCATGCTCCCTCATT+GGG + chr7.4:6887902-6887921 None:intergenic 50.0%
CCCACACCAACTAGTAATGG+CGG - chr7.4:6887070-6887089 MS.gene007264:intron 50.0%
CGGGTAAAAACGGACGGATA+CGG + chr7.4:6887387-6887406 None:intergenic 50.0%
GAAGTGCAGGTGATTGTTGG+TGG + chr7.4:6886276-6886295 None:intergenic 50.0%
GATCCACCGCCATTACTAGT+TGG + chr7.4:6887079-6887098 None:intergenic 50.0%
GGGTAAAAACGGACGGATAC+GGG + chr7.4:6887386-6887405 None:intergenic 50.0%
GTCAAGTCACTTAGCTGAGG+TGG - chr7.4:6888274-6888293 MS.gene007264:CDS 50.0%
TAAAAAGTGGCGGTGTGCGA+GGG - chr7.4:6886970-6886989 MS.gene007264:intron 50.0%
TATTGGCCATGCTCCCTCAT+TGG + chr7.4:6887903-6887922 None:intergenic 50.0%
TCTGGCAAATGATGTCCCAC+TGG + chr7.4:6887725-6887744 None:intergenic 50.0%
! ACCGCCATTACTAGTTGGTG+TGG + chr7.4:6887074-6887093 None:intergenic 50.0%
! CCGCCATTACTAGTTGGTGT+GGG + chr7.4:6887073-6887092 None:intergenic 50.0%
! CTACGGATTGTTCGGTTTCC+GGG - chr7.4:6887476-6887495 MS.gene007264:CDS 50.0%
! GTGTTGCAAAGTGGGTACTG+GGG - chr7.4:6886899-6886918 MS.gene007264:intron 50.0%
!! CTAGTAGCTTTGGGTACCGA+CGG - chr7.4:6887339-6887358 MS.gene007264:intron 50.0%
!! GTGACTTTGGTGTTGGGTTG+AGG + chr7.4:6886310-6886329 None:intergenic 50.0%
!! GTTGGTGGTGTTGATGAGCA+GGG + chr7.4:6886261-6886280 None:intergenic 50.0%
!! TGTTGGTGGTGTTGATGAGC+AGG + chr7.4:6886262-6886281 None:intergenic 50.0%
AAACCGAGATGCAAACGGGC+GGG + chr7.4:6887456-6887475 None:intergenic 55.0%
CACTTAGCTGAGGTGGCATC+AGG - chr7.4:6888281-6888300 MS.gene007264:CDS 55.0%
CTTAGCTGAGGTGGCATCAG+GGG - chr7.4:6888283-6888302 MS.gene007264:CDS 55.0%
GAAACCGAGATGCAAACGGG+CGG + chr7.4:6887457-6887476 None:intergenic 55.0%
GAGATACGGACCCCCAATGA+GGG - chr7.4:6887887-6887906 MS.gene007264:CDS 55.0%
GTAAAAAGTGGCGGTGTGCG+AGG - chr7.4:6886969-6886988 MS.gene007264:intron 55.0%
GTACTGGGGAAGAGGAGCAA+TGG - chr7.4:6886913-6886932 MS.gene007264:intron 55.0%
GTTGGTGTGGGAAGTTCCAC+AGG + chr7.4:6887061-6887080 None:intergenic 55.0%
TGAGATACGGACCCCCAATG+AGG - chr7.4:6887886-6887905 MS.gene007264:CDS 55.0%
TGGGGGTCCGTATCTCAGTT+TGG + chr7.4:6887883-6887902 None:intergenic 55.0%
TTGGCCATGCTCCCTCATTG+GGG + chr7.4:6887901-6887920 None:intergenic 55.0%
!! CAAAGTGGGTACTGGGGAAG+AGG - chr7.4:6886905-6886924 MS.gene007264:intron 55.0%
AACGGACGGATACGGGTGCA+AGG + chr7.4:6887379-6887398 None:intergenic 60.0%
ACAAGATTTCCCGACCCGCC+CGG + chr7.4:6887497-6887516 None:intergenic 60.0%
ATCCAGAGCCAGGTCGTTGC+CGG - chr7.4:6886936-6886955 MS.gene007264:intron 60.0%
CGGATTGTTCGGTTTCCGGG+CGG - chr7.4:6887479-6887498 MS.gene007264:CDS 60.0%
GGAGCAATGGATCCAGAGCC+AGG - chr7.4:6886926-6886945 MS.gene007264:intron 60.0%
GGATTGTTCGGTTTCCGGGC+GGG - chr7.4:6887480-6887499 MS.gene007264:CDS 60.0%
GGTCGTTGCCGGAGAACAGA+CGG - chr7.4:6886947-6886966 MS.gene007264:intron 60.0%
TCTGTTCTCCGGCAACGACC+TGG + chr7.4:6886947-6886966 None:intergenic 60.0%
TGGCCATGCTCCCTCATTGG+GGG + chr7.4:6887900-6887919 None:intergenic 60.0%
AGCTGAGGTGGCATCAGGGG+CGG - chr7.4:6888286-6888305 MS.gene007264:CDS 65.0%
GGACCCCCAATGAGGGAGCA+TGG - chr7.4:6887894-6887913 MS.gene007264:CDS 65.0%
TGTGCGAGGGATGTGGTGGC+TGG - chr7.4:6886983-6887002 MS.gene007264:intron 65.0%
TGTTCGGTTTCCGGGCGGGT+CGG - chr7.4:6887484-6887503 MS.gene007264:CDS 65.0%
CGAGATGCAAACGGGCGGGC+CGG + chr7.4:6887452-6887471 None:intergenic 70.0%
CGGCCCGCCCGTTTGCATCT+CGG - chr7.4:6887450-6887469 MS.gene007264:CDS 70.0%
CTCCGGCAACGACCTGGCTC+TGG + chr7.4:6886941-6886960 None:intergenic 70.0%
GCGGTGTGCGAGGGATGTGG+TGG - chr7.4:6886979-6886998 MS.gene007264:intron 70.0%
GTGGCGGTGTGCGAGGGATG+TGG - chr7.4:6886976-6886995 MS.gene007264:intron 70.0%
GTTCGGTTTCCGGGCGGGTC+GGG - chr7.4:6887485-6887504 MS.gene007264:CDS 70.0%
TGCAAACGGGCGGGCCGGTT+TGG + chr7.4:6887447-6887466 None:intergenic 70.0%
!! GGCGGGCCGGTTTGGTCAAC+GGG + chr7.4:6887439-6887458 None:intergenic 70.0%
!! GGGCGGGCCGGTTTGGTCAA+CGG + chr7.4:6887440-6887459 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 6886175 6888378 6886175 ID=MS.gene007264
chr7.4 mRNA 6886175 6888378 6886175 ID=MS.gene007264.t1;Parent=MS.gene007264
chr7.4 exon 6888180 6888378 6888180 ID=MS.gene007264.t1.exon1;Parent=MS.gene007264.t1
chr7.4 CDS 6888180 6888378 6888180 ID=cds.MS.gene007264.t1;Parent=MS.gene007264.t1
chr7.4 exon 6887861 6888037 6887861 ID=MS.gene007264.t1.exon2;Parent=MS.gene007264.t1
chr7.4 CDS 6887861 6888037 6887861 ID=cds.MS.gene007264.t1;Parent=MS.gene007264.t1
chr7.4 exon 6887362 6887653 6887362 ID=MS.gene007264.t1.exon3;Parent=MS.gene007264.t1
chr7.4 CDS 6887362 6887653 6887362 ID=cds.MS.gene007264.t1;Parent=MS.gene007264.t1
chr7.4 exon 6886175 6886260 6886175 ID=MS.gene007264.t1.exon4;Parent=MS.gene007264.t1
chr7.4 CDS 6886175 6886260 6886175 ID=cds.MS.gene007264.t1;Parent=MS.gene007264.t1
Gene Sequence

>MS.gene007264

ATGGACTTTCATACGAAACAATGGAGAAACCAACAGCATGAGTCAGAGAAACAACATTCCACAAAGATGCCAAAACTTCTTCACCCTGCTCATCAACACCACCAACAATCACCTGCACTTCCTTTGTTTGTACCTCAACCCAACACCAAAGTCACCAACTTGTCAGATTCAGCATTAGCTTCCAACAACAGATTTCCTAGTAAGTTATTTTGAGTTAGCTTAACGCATTAGTTACTTACTTACTACAAGTACTTTTTTGTTTCAAGTGTGCTTTCAAAGTTACTACTACTACATCTTTCTTCTTTTGGATCCAACACTTTAACAAGTTCTTACATTATTAGGAATGGGAATGGGAAGCCATTTCAGCTTATCTCAGTGGCAAGAACTTGAGTTACAGGCTTTGATATTTAGATACATGTTGGTTGGTGCTTCTGTTCCTCCTGAACTTCTTCAACCTATCAAGAAAAGTCTTCTTCATTCATCTCCTTATTTTCTTCATCATTATCAACCTACAGCATGTAAGTTTCATTCTACTTGGTTTCTTACACGGTTTTCCATATCACATACTCCAAAACAAAACCAAAAAACACACTTTCTTTTCTTCGTGTTTTGGGATATGATATAATTGAATATTCCCCCAGTAACATTATTTTTACTTGGATGAGATAAAAACAACAAAGTGTAGTACATTATTTTAAAAATGTTTTTGTTGTTGTTTTTATTAGTGTTGCAAAGTGGGTACTGGGGAAGAGGAGCAATGGATCCAGAGCCAGGTCGTTGCCGGAGAACAGACGGTAAAAAGTGGCGGTGTGCGAGGGATGTGGTGGCTGGACAAAAGTACTGTGAAAGACACATGCATCGTGGTAGAAACCGTTCAAGAAAGCCTGTGGAACTTCCCACACCAACTAGTAATGGCGGTGGATCTTCATCTTTCTCTGCTTTGTCTTCTATTTCTTCACAGCCTCTTGTTACTTCCTCCTCATTCAAATCTCCTTTTGATCTTCACTTTAATCAACGGTAATCTTCTTCTCTTCTCTTCTCTTCTCTCTCTTAATTTACTAATTAAATGACAATGGTTTGCTAGTAGTAGCCTAATGAGTAATTACTTGTAGTATTTTTTTATTTTTGATTTGAAGTTTAATAATGACTTGTAACAAAATACTACTAGTAGCTTTGGGTACCGACGGTTCAATTATTTGCACCTTGCACCCGTATCCGTCCGTTTTTACCCGTGAAAACCGATTTTTTAACTATATTTAGACCCGTTGACCAAACCGGCCCGCCCGTTTGCATCTCGGTTTCTACGGATTGTTCGGTTTCCGGGCGGGTCGGGAAATCTTGTCCAGCCCTAGCACATACTATTGTTTAGGTTGCCACTTGGTTCAAGTTTGAAATTATGCCGTTAGTAGATTATACATAAATTTCATTTAAATCTTTAATAAGATTGGTGGTTCTTCTTCAACTTGCATCTAATTATGTTGATTTGATATAAGCTTTGTTTTTGAGAAGATTGAGTCATTGTAATTTGTGTATGAAGATCATTTAATCCAGTGGGACATCATTTGCCAGAACAACTTTTTTTTCTGTCTTTTGTCATCTTTTTAAGATGGGTTCATTGACCTTCAAAAAGTAAGTTTGAATTTGATGCATAACCAAATAATGGAAGGTGTAATTAGTTCAACATTGTGACAGGACTTGACTGATACCAAACTGAGATACGGACCCCCAATGAGGGAGCATGGCCAATATGTTTCATACTTGGCAAATAATGAAGCCTTTGGATTTGGTTCAAATAATCCCCAACCGTTTCTTTCTAAAACTAAATTTAGAAATCATCAATCATGCCGTCCATATGATTTTTAATCTTTATGGACACTCTTGATTGAAACTTAAGTCCAATGTCGTACTACAATAGTATGAAGTACTAGTAGTAATTATAAGTGGGTCATACCTAATTAACTTTAAGGCTTAAATGCACTTTTGACCCCTATGTTTCAAATAGTTGCGATTTTGACCCCCTAAGAAAAAAAATGGATATTTTGACCCCCTATGTTTTGGCCCTCTTTTGATTTGCTGATTTTGACCATCAACGTCCATGTGTCAAGTCACTTAGCTGAGGTGGCATCAGGGGCGGATCCAGACTTTAGATACATGTGGAGCTAAAATTTCAAAAAAAACAATAATGCAAAATTTATTCACGTCGA
Protein sequence

>MS.gene007264.t1

MDFHTKQWRNQQHESEKQHSTKMPKLLHPAHQHHQQSPALPLFVPQPNTKVTNLSDSALASNNRFPRMGMGSHFSLSQWQELELQALIFRYMLVGASVPPELLQPIKKSLLHSSPYFLHHYQPTALLQSGYWGRGAMDPEPGRCRRTDGKKWRCARDVVAGQKYCERHMHRGRNRSRKPVELPTPTSNGGGSSSFSALSSISSQPLVTSSSFKSPFDLHFNQRWHQGRIQTLDTCGAKISKKTIMQNLFTS