AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007333

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007333.t1 MTR_7g103340 98.630 146 2 0 1 146 1 146 4.29e-105 300
MS.gene007333.t1 MTR_1g061660 63.758 149 48 3 1 148 1 144 3.62e-60 186
MS.gene007333.t1 MTR_8g468210 45.185 135 58 2 21 148 44 169 1.53e-31 114
MS.gene007333.t1 MTR_6g007647 58.511 94 32 1 62 148 32 125 6.82e-31 111
MS.gene007333.t1 MTR_6g007647 58.511 94 32 1 62 148 66 159 9.42e-31 112
MS.gene007333.t1 MTR_5g039000 50.476 105 45 1 51 148 65 169 2.58e-29 108
MS.gene007333.t1 MTR_3g080100 57.303 89 38 0 56 144 13 101 5.27e-28 103
MS.gene007333.t1 MTR_4g107650 56.383 94 34 1 62 148 94 187 7.42e-28 105
MS.gene007333.t1 MTR_3g080100 57.303 89 38 0 56 144 52 140 1.15e-27 103
MS.gene007333.t1 MTR_7g010020 56.311 103 37 2 51 145 67 169 1.58e-27 103
MS.gene007333.t1 MTR_6g011610 57.831 83 35 0 62 144 95 177 2.27e-27 103
MS.gene007333.t1 MTR_6g011610 57.831 83 35 0 62 144 76 158 2.83e-27 102
MS.gene007333.t1 MTR_8g089895 58.025 81 34 0 62 142 53 133 1.47e-26 101
MS.gene007333.t1 MTR_3g086790 53.125 96 43 1 41 134 36 131 3.44e-26 100
MS.gene007333.t1 MTR_5g038280 54.444 90 34 1 63 145 87 176 8.98e-25 97.1
MS.gene007333.t1 MTR_8g469430 51.613 93 38 1 63 148 89 181 8.60e-24 94.0
MS.gene007333.t1 MTR_3g092150 53.750 80 37 0 65 144 40 119 1.87e-23 92.4
MS.gene007333.t1 MTR_8g026960 50.000 82 34 1 67 141 38 119 5.80e-21 85.5
MS.gene007333.t1 MTR_4g097600 46.667 90 48 0 56 145 120 209 3.03e-19 81.6
MS.gene007333.t1 MTR_5g019680 46.512 86 46 0 63 148 53 138 3.16e-18 77.8
MS.gene007333.t1 MTR_1g054285 44.444 90 50 0 56 145 147 236 8.90e-18 78.2
MS.gene007333.t1 MTR_5g019650 49.398 83 42 0 63 145 53 135 1.00e-17 76.6
MS.gene007333.t1 MTR_4g100550 45.783 83 45 0 63 145 120 202 1.64e-17 77.0
MS.gene007333.t1 MTR_1g017090 43.434 99 56 0 47 145 114 212 1.95e-17 77.0
MS.gene007333.t1 MTR_7g093430 44.318 88 49 0 58 145 155 242 2.45e-17 77.0
MS.gene007333.t1 MTR_2g061030 42.105 95 55 0 51 145 188 282 2.96e-16 74.3
MS.gene007333.t1 MTR_5g014890 45.556 90 48 1 56 145 117 205 4.51e-16 73.2
MS.gene007333.t1 MTR_5g013010 45.783 83 45 0 63 145 136 218 1.02e-15 72.4
MS.gene007333.t1 MTR_4g084750 42.391 92 52 1 54 145 57 147 5.94e-15 68.9
MS.gene007333.t1 MTR_8g006705 43.373 83 47 0 63 145 146 228 1.17e-14 69.7
MS.gene007333.t1 MTR_4g126900 45.000 80 44 0 66 145 91 170 3.62e-14 67.4
MS.gene007333.t1 MTR_5g029320 39.216 102 59 1 51 149 77 178 1.66e-13 67.0
MS.gene007333.t1 MTR_6g477820 43.956 91 49 1 61 149 28 118 2.46e-13 66.6
MS.gene007333.t1 MTR_2g101720 45.122 82 44 1 66 147 145 225 2.93e-12 63.5
MS.gene007333.t1 MTR_2g038625 33.793 145 86 4 4 145 27 164 3.82e-12 61.6
MS.gene007333.t1 MTR_2g038580 33.793 145 86 4 4 145 27 164 3.82e-12 61.6
MS.gene007333.t1 MTR_3g103590 39.535 86 51 1 50 135 133 217 5.99e-12 62.0
MS.gene007333.t1 MTR_1g101280 38.889 90 52 1 61 147 54 143 2.77e-11 60.5
MS.gene007333.t1 MTR_5g013010 53.846 52 24 0 63 114 136 187 3.37e-11 58.9
MS.gene007333.t1 MTR_7g027265 39.080 87 53 0 63 149 20 106 5.73e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007333.t1 AT5G03790 57.554 139 48 3 16 146 25 160 3.55e-44 144
MS.gene007333.t1 AT5G03790 57.971 138 47 3 16 145 25 159 7.52e-44 144
MS.gene007333.t1 AT2G36610 48.125 160 65 3 7 148 5 164 4.42e-39 130
MS.gene007333.t1 AT1G69780 55.000 100 38 1 56 148 78 177 3.84e-30 110
MS.gene007333.t1 AT5G15150 53.333 105 40 2 51 146 101 205 3.15e-28 106
MS.gene007333.t1 AT5G65310 59.259 81 33 0 62 142 53 133 1.13e-27 104
MS.gene007333.t1 AT5G65310 57.831 83 35 0 62 144 71 153 1.22e-27 104
MS.gene007333.t1 AT4G40060 50.450 111 46 2 33 134 20 130 4.00e-27 102
MS.gene007333.t1 AT2G22430 67.606 71 23 0 62 132 61 131 4.86e-27 103
MS.gene007333.t1 AT3G01470 66.197 71 24 0 62 132 67 137 5.04e-27 102
MS.gene007333.t1 AT3G01220 49.074 108 51 2 38 141 58 165 4.57e-25 97.4
MS.gene007333.t1 AT2G46680 48.421 95 42 1 58 145 27 121 2.10e-24 95.1
MS.gene007333.t1 AT4G36740 51.613 93 45 0 56 148 48 140 2.21e-24 94.0
MS.gene007333.t1 AT4G36740 52.747 91 43 0 56 146 49 139 2.60e-24 94.0
MS.gene007333.t1 AT1G26960 52.174 92 44 0 51 142 59 150 2.82e-24 94.7
MS.gene007333.t1 AT2G46680 47.126 87 46 0 58 144 27 113 5.14e-24 94.0
MS.gene007333.t1 AT3G61890 49.425 87 37 1 66 145 33 119 1.41e-22 89.7
MS.gene007333.t1 AT2G18550 39.344 122 73 1 27 148 26 146 2.72e-21 86.3
MS.gene007333.t1 AT1G27045 57.971 69 29 0 63 131 25 93 1.03e-20 83.6
MS.gene007333.t1 AT1G27045 57.971 69 29 0 63 131 25 93 1.03e-20 83.6
MS.gene007333.t1 AT1G27045 57.971 69 29 0 63 131 17 85 1.20e-20 83.2
MS.gene007333.t1 AT1G27045 57.971 69 29 0 63 131 68 136 1.60e-20 84.3
MS.gene007333.t1 AT1G27045 57.971 69 29 0 63 131 68 136 2.61e-20 84.3
MS.gene007333.t1 AT5G66700 48.810 84 43 0 63 146 71 154 8.65e-20 82.4
MS.gene007333.t1 AT5G06710 43.299 97 55 0 49 145 176 272 8.17e-18 78.6
MS.gene007333.t1 AT5G53980 43.529 85 48 0 61 145 9 93 2.16e-17 74.3
MS.gene007333.t1 AT4G16780 40.860 93 54 1 56 148 123 214 6.19e-16 72.8
MS.gene007333.t1 AT2G22800 45.783 83 45 0 63 145 113 195 1.02e-15 72.4
MS.gene007333.t1 AT3G60390 41.111 90 53 0 56 145 155 244 2.82e-15 71.6
MS.gene007333.t1 AT5G47370 40.659 91 54 0 56 146 123 213 2.85e-15 71.2
MS.gene007333.t1 AT4G37790 45.783 83 45 0 63 145 126 208 2.98e-15 70.9
MS.gene007333.t1 AT2G44910 43.373 83 47 0 63 145 163 245 5.25e-15 70.9
MS.gene007333.t1 AT2G01430 44.444 90 50 0 56 145 132 221 6.39e-15 70.1
MS.gene007333.t1 AT4G17460 40.909 88 52 0 57 144 129 216 9.26e-15 69.7
MS.gene007333.t1 AT1G79840 40.659 91 52 1 57 145 96 186 9.91e-14 67.8
MS.gene007333.t1 AT1G79840 40.659 91 52 1 57 145 96 186 1.00e-13 67.8
MS.gene007333.t1 AT1G79840 40.659 91 52 1 57 145 125 215 1.01e-13 67.8
MS.gene007333.t1 AT1G79840 40.659 91 52 1 57 145 38 128 1.03e-13 67.8
MS.gene007333.t1 AT1G79840 40.659 91 52 1 57 145 38 128 1.03e-13 67.8
MS.gene007333.t1 AT1G79840 40.659 91 52 1 57 145 96 186 1.07e-13 67.4
MS.gene007333.t1 AT1G73360 45.977 87 45 1 61 145 31 117 1.92e-13 67.0
MS.gene007333.t1 AT5G06710 48.485 66 34 0 49 114 176 241 9.21e-13 64.3
MS.gene007333.t1 AT5G46880 41.176 85 50 0 61 145 112 196 4.04e-12 63.2
MS.gene007333.t1 AT5G46880 41.176 85 50 0 61 145 112 196 4.04e-12 63.2
MS.gene007333.t1 AT5G46880 41.176 85 50 0 61 145 112 196 4.04e-12 63.2
MS.gene007333.t1 AT1G70920 35.484 124 74 2 22 145 4 121 6.97e-12 60.5
MS.gene007333.t1 AT1G70920 38.776 98 60 0 48 145 54 151 9.27e-12 60.5
MS.gene007333.t1 AT1G17920 43.750 80 45 0 66 145 27 106 1.74e-11 61.2
MS.gene007333.t1 AT1G17920 43.750 80 45 0 66 145 27 106 1.74e-11 61.2
MS.gene007333.t1 AT3G61150 36.364 99 61 1 51 147 99 197 2.22e-11 60.8
MS.gene007333.t1 AT4G21750 37.778 90 56 0 59 148 61 150 3.77e-11 60.5
MS.gene007333.t1 AT4G21750 37.778 90 56 0 59 148 61 150 3.77e-11 60.5
MS.gene007333.t1 AT4G21750 37.778 90 56 0 59 148 61 150 3.77e-11 60.5
MS.gene007333.t1 AT4G21750 37.778 90 56 0 59 148 61 150 3.77e-11 60.5

Find 34 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCTTCATCTTCCTTTC+AGG 0.091903 7.4:+7854073 None:intergenic
TCCACCTTGTACGCCTATTT+TGG 0.171135 7.4:+7854008 None:intergenic
TTGCTGTTTGGTTCCAAAAT+AGG 0.192526 7.4:-7854021 MS.gene007333:CDS
GATAATTAGGTACCTCTTCT+TGG 0.238895 7.4:+7853909 None:intergenic
GGTCTAACTTTATTTCTTCT+TGG 0.251946 7.4:+7854095 None:intergenic
TTTGGAACCAAACAGCAATT+TGG 0.278055 7.4:+7854026 None:intergenic
TATAACAACACCCCTTATTC+AGG 0.354916 7.4:-7854767 MS.gene007333:intron
AAAGCTTCATCTTCCTTTCA+GGG 0.372499 7.4:+7854074 None:intergenic
AACCTCGCCAAATTGCTGTT+TGG 0.376064 7.4:-7854033 MS.gene007333:CDS
GGAAATGAGTCAACCGTCAT+TGG 0.398063 7.4:-7854236 MS.gene007333:CDS
ATGAAGCTTTCAAGAGAGCT+AGG 0.447660 7.4:-7854061 MS.gene007333:CDS
ATAGTTGTAGAAGAAGCTTA+AGG 0.450197 7.4:+7854787 None:intergenic
ATGGATGAAATGAACATTGA+TGG 0.450308 7.4:-7854184 MS.gene007333:CDS
AAGTAACCAGATTGATTCAC+TGG 0.476965 7.4:-7854128 MS.gene007333:CDS
TCTTCTCCAGTGAATCAATC+TGG 0.478388 7.4:+7854122 None:intergenic
AAACAGCAATTTGGCGAGGT+TGG 0.478872 7.4:+7854035 None:intergenic
GGTTCCAAAATAGGCGTACA+AGG 0.492609 7.4:-7854012 MS.gene007333:CDS
ATGTTCATTTCATCCATAGC+TGG 0.495881 7.4:+7854190 None:intergenic
AGTGAAAGCCTAAAATGGAT+TGG 0.500919 7.4:-7854850 None:intergenic
CTTAAGGAAGACTCGGTATG+AGG 0.509787 7.4:+7854803 None:intergenic
GAAATGAGTCAACCGTCATT+GGG 0.510295 7.4:-7854235 MS.gene007333:CDS
AGAATTTGATGTTGTCTCCA+AGG 0.530430 7.4:-7853945 MS.gene007333:CDS
GACTCGGTATGAGGAACAAA+GGG 0.536486 7.4:+7854812 None:intergenic
ACAAAGGTTACTTCCAGCTA+TGG 0.541285 7.4:-7854203 MS.gene007333:CDS
AAATAAAGTTAGACCCTGAA+AGG 0.563460 7.4:-7854087 MS.gene007333:CDS
TGAAGCTTTCAAGAGAGCTA+GGG 0.584376 7.4:-7854060 MS.gene007333:CDS
AGACTCGGTATGAGGAACAA+AGG 0.604496 7.4:+7854811 None:intergenic
TGTTGTGTCTCACCCAATGA+CGG 0.608704 7.4:+7854223 None:intergenic
AAATAGGCGTACAAGGTGGA+AGG 0.609407 7.4:-7854005 MS.gene007333:CDS
AAAACAGAAACTCCAAGAAG+AGG 0.620624 7.4:-7853921 MS.gene007333:intron
GAAGAAGCTTAAGGAAGACT+CGG 0.638190 7.4:+7854796 None:intergenic
AACCAAACAGCAATTTGGCG+AGG 0.655043 7.4:+7854031 None:intergenic
TCCAAAATAGGCGTACAAGG+TGG 0.664815 7.4:-7854009 MS.gene007333:CDS
CATTGGGTGAGACACAACAA+AGG 0.711095 7.4:-7854219 MS.gene007333:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAGAAAAAAAAG+AGG + chr7.4:7853284-7853303 None:intergenic 10.0%
!! AAAAAAAAAGAAAAAAAAGA+GGG + chr7.4:7853283-7853302 None:intergenic 10.0%
!! CTGTATAAAAAAATTAATTA+TGG - chr7.4:7854768-7854787 MS.gene007333:CDS 10.0%
!! AAAAAAAAGAAAAAAAAGAG+GGG + chr7.4:7853282-7853301 None:intergenic 15.0%
!! AAAAAAATTAATTATGGACA+TGG - chr7.4:7854774-7854793 MS.gene007333:CDS 15.0%
!! AAAAATAGCTAAAAAAACAT+TGG + chr7.4:7853168-7853187 None:intergenic 15.0%
!! AGAAAAAAAAATTGTATGAT+AGG - chr7.4:7853519-7853538 MS.gene007333:intron 15.0%
!! GAAAAAAAAATTGTATGATA+GGG - chr7.4:7853520-7853539 MS.gene007333:intron 15.0%
!! TTATTCAAATTTATTGTCAT+TGG - chr7.4:7853601-7853620 MS.gene007333:intron 15.0%
!!! ATAATTCAACTTCTTTTTAT+AGG + chr7.4:7854140-7854159 None:intergenic 15.0%
!!! GAAAAATAGTAATTTTTGTA+AGG + chr7.4:7854642-7854661 None:intergenic 15.0%
!! AATAATACAACTCTAACAAA+TGG + chr7.4:7854457-7854476 None:intergenic 20.0%
!! CAAAAAATCCAAAGATAATT+AGG + chr7.4:7853989-7854008 None:intergenic 20.0%
!! TATAATGTAGAATATGTGTA+AGG + chr7.4:7854678-7854697 None:intergenic 20.0%
!!! AATTCAACTTTTTTCCTAAT+AGG - chr7.4:7854246-7854265 MS.gene007333:intron 20.0%
!!! ATTTGAAAACTACTCAATTT+TGG - chr7.4:7853338-7853357 MS.gene007333:intron 20.0%
!!! TATCTTTGGATTTTTTGTAT+AGG - chr7.4:7853992-7854011 MS.gene007333:CDS 20.0%
!!! TGTTTTTTTTACCTGAATAA+GGG + chr7.4:7853129-7853148 None:intergenic 20.0%
!!! TTATGTGCTTTTTTCTATTT+CGG - chr7.4:7854738-7854757 MS.gene007333:intron 20.0%
! AAAAACATTGGAGTATAAAG+AGG + chr7.4:7853156-7853175 None:intergenic 25.0%
! AACAATTTATGAGATGCATA+TGG - chr7.4:7853456-7853475 MS.gene007333:intron 25.0%
! TAAAAATCACATAATGCAAG+AGG + chr7.4:7854115-7854134 None:intergenic 25.0%
! TACATAAATCAACCATTGTA+GGG + chr7.4:7854820-7854839 None:intergenic 25.0%
!! TGCTTTTCAATTCATATAGA+AGG - chr7.4:7853303-7853322 MS.gene007333:intron 25.0%
!!! GTGTTTTTTTTACCTGAATA+AGG + chr7.4:7853130-7853149 None:intergenic 25.0%
!!! GTTTTTTTTACCTGAATAAG+GGG + chr7.4:7853128-7853147 None:intergenic 25.0%
!!! TATGTGCTTTTTTCTATTTC+GGG - chr7.4:7854739-7854758 MS.gene007333:intron 25.0%
AAAAAAGAGGGGAAATAACT+TGG + chr7.4:7853271-7853290 None:intergenic 30.0%
AAAAAGAGGGGAAATAACTT+GGG + chr7.4:7853270-7853289 None:intergenic 30.0%
AAATAAAGTTAGACCCTGAA+AGG - chr7.4:7853795-7853814 MS.gene007333:intron 30.0%
ATAGTTGTAGAAGAAGCTTA+AGG + chr7.4:7853098-7853117 None:intergenic 30.0%
ATGGATGAAATGAACATTGA+TGG - chr7.4:7853698-7853717 MS.gene007333:intron 30.0%
CATAAGTGAGTGTCTAATTA+AGG + chr7.4:7853367-7853386 None:intergenic 30.0%
CCTCAAACTTGAAATGTAAT+AGG + chr7.4:7854335-7854354 None:intergenic 30.0%
CTACATAAATCAACCATTGT+AGG + chr7.4:7854821-7854840 None:intergenic 30.0%
GAGAAATGGTATGAATATGA+TGG - chr7.4:7853563-7853582 MS.gene007333:intron 30.0%
! GGTCTAACTTTATTTCTTCT+TGG + chr7.4:7853790-7853809 None:intergenic 30.0%
!! AAGAGGTACCTAATTATCTT+TGG - chr7.4:7853978-7853997 MS.gene007333:CDS 30.0%
!! CCTATTACATTTCAAGTTTG+AGG - chr7.4:7854332-7854351 MS.gene007333:intron 30.0%
!!! ATGTGCTTTTTTCTATTTCG+GGG - chr7.4:7854740-7854759 MS.gene007333:intron 30.0%
!!! TTGGATTTTTTGTATAGGCT+AGG - chr7.4:7853997-7854016 MS.gene007333:CDS 30.0%
AAAACAGAAACTCCAAGAAG+AGG - chr7.4:7853961-7853980 MS.gene007333:CDS 35.0%
AAAGCTTCATCTTCCTTTCA+GGG + chr7.4:7853811-7853830 None:intergenic 35.0%
AAATCAGACTTACGCCTATT+AGG + chr7.4:7854263-7854282 None:intergenic 35.0%
AACCATTGTAGGGTAATACT+AGG + chr7.4:7854810-7854829 None:intergenic 35.0%
AACCTAGTATTACCCTACAA+TGG - chr7.4:7854805-7854824 MS.gene007333:CDS 35.0%
AAGAAATGAAGGCAGAGAAA+TGG - chr7.4:7853549-7853568 MS.gene007333:intron 35.0%
AGAATTTGATGTTGTCTCCA+AGG - chr7.4:7853937-7853956 MS.gene007333:CDS 35.0%
ATAGGTCTCTAAGCTTCTAA+AGG + chr7.4:7854317-7854336 None:intergenic 35.0%
ATGTTCATTTCATCCATAGC+TGG + chr7.4:7853695-7853714 None:intergenic 35.0%
GATAATTAGGTACCTCTTCT+TGG + chr7.4:7853976-7853995 None:intergenic 35.0%
GTATGCTGGAAAATTAGGAA+TGG - chr7.4:7853625-7853644 MS.gene007333:intron 35.0%
TAGGGTGTACAAAGAAATGA+AGG - chr7.4:7853538-7853557 MS.gene007333:intron 35.0%
TATAACAACACCCCTTATTC+AGG - chr7.4:7853115-7853134 MS.gene007333:intron 35.0%
TTATTGTCATTGGTGTATGC+TGG - chr7.4:7853611-7853630 MS.gene007333:intron 35.0%
TTGGTGTATGCTGGAAAATT+AGG - chr7.4:7853620-7853639 MS.gene007333:intron 35.0%
! AAGTAACCAGATTGATTCAC+TGG - chr7.4:7853754-7853773 MS.gene007333:intron 35.0%
! TTTGGAACCAAACAGCAATT+TGG + chr7.4:7853859-7853878 None:intergenic 35.0%
!! TTGCTGTTTGGTTCCAAAAT+AGG - chr7.4:7853861-7853880 MS.gene007333:intron 35.0%
!! TTGGAGTTTCTGTTTTTCCT+TGG + chr7.4:7853957-7853976 None:intergenic 35.0%
ACAAAGGTTACTTCCAGCTA+TGG - chr7.4:7853679-7853698 MS.gene007333:intron 40.0%
ATGAAGCTTTCAAGAGAGCT+AGG - chr7.4:7853821-7853840 MS.gene007333:intron 40.0%
GAAAGCTTCATCTTCCTTTC+AGG + chr7.4:7853812-7853831 None:intergenic 40.0%
GAAATGAGTCAACCGTCATT+GGG - chr7.4:7853647-7853666 MS.gene007333:intron 40.0%
GAAGAAGCTTAAGGAAGACT+CGG + chr7.4:7853089-7853108 None:intergenic 40.0%
TCTTCTCCAGTGAATCAATC+TGG + chr7.4:7853763-7853782 None:intergenic 40.0%
TGAAGCTTTCAAGAGAGCTA+GGG - chr7.4:7853822-7853841 MS.gene007333:intron 40.0%
! ATAATGCAAGAGGTACATGG+AGG + chr7.4:7854105-7854124 None:intergenic 40.0%
! ATTCTAGAGAGAGAGAGAGA+AGG + chr7.4:7853220-7853239 None:intergenic 40.0%
! CACATAATGCAAGAGGTACA+TGG + chr7.4:7854108-7854127 None:intergenic 40.0%
! TAATGCAAGAGGTACATGGA+GGG + chr7.4:7854104-7854123 None:intergenic 40.0%
AAACAGCAATTTGGCGAGGT+TGG + chr7.4:7853850-7853869 None:intergenic 45.0%
AAATAGGCGTACAAGGTGGA+AGG - chr7.4:7853877-7853896 MS.gene007333:intron 45.0%
AACCAAACAGCAATTTGGCG+AGG + chr7.4:7853854-7853873 None:intergenic 45.0%
CATTGGGTGAGACACAACAA+AGG - chr7.4:7853663-7853682 MS.gene007333:intron 45.0%
GGAAATGAGTCAACCGTCAT+TGG - chr7.4:7853646-7853665 MS.gene007333:intron 45.0%
GGTTCCAAAATAGGCGTACA+AGG - chr7.4:7853870-7853889 MS.gene007333:intron 45.0%
TCCAAAATAGGCGTACAAGG+TGG - chr7.4:7853873-7853892 MS.gene007333:intron 45.0%
TGTTGTGTCTCACCCAATGA+CGG + chr7.4:7853662-7853681 None:intergenic 45.0%
! AACCTCGCCAAATTGCTGTT+TGG - chr7.4:7853849-7853868 MS.gene007333:intron 45.0%
! AGACTCGGTATGAGGAACAA+AGG + chr7.4:7853074-7853093 None:intergenic 45.0%
! CTTAAGGAAGACTCGGTATG+AGG + chr7.4:7853082-7853101 None:intergenic 45.0%
! GACTCGGTATGAGGAACAAA+GGG + chr7.4:7853073-7853092 None:intergenic 45.0%
! TCCACCTTGTACGCCTATTT+TGG + chr7.4:7853877-7853896 None:intergenic 45.0%
!!! ATTTATTTTTTTACTTTTTT+TGG + chr7.4:7854391-7854410 None:intergenic 5.0%
!!! ATTTTATGTTTTTTTTTTTT+TGG + chr7.4:7854179-7854198 None:intergenic 5.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 7853046 7854858 7853046 ID=MS.gene007333
chr7.4 mRNA 7853046 7854858 7853046 ID=MS.gene007333.t1;Parent=MS.gene007333
chr7.4 exon 7854768 7854858 7854768 ID=MS.gene007333.t1.exon1;Parent=MS.gene007333.t1
chr7.4 CDS 7854768 7854858 7854768 ID=cds.MS.gene007333.t1;Parent=MS.gene007333.t1
chr7.4 exon 7853922 7854262 7853922 ID=MS.gene007333.t1.exon2;Parent=MS.gene007333.t1
chr7.4 CDS 7853922 7854262 7853922 ID=cds.MS.gene007333.t1;Parent=MS.gene007333.t1
chr7.4 exon 7853046 7853063 7853046 ID=MS.gene007333.t1.exon3;Parent=MS.gene007333.t1
chr7.4 CDS 7853046 7853063 7853046 ID=cds.MS.gene007333.t1;Parent=MS.gene007333.t1
Gene Sequence

>MS.gene007333

ATGGATTGGAATGAGAGCACAAGACCCTTTGTTCCTCATACCGAGTCTTCCTTAAGCTTCTTCTACAACTATAACAACACCCCTTATTCAGGTAAAAAAAACACTGACCTCTTTATACTCCAATGTTTTTTTAGCTATTTTTACTCATTTTAGTTTACAAATCTTGAGTCACCTTCTCTCTCTCTCTCTAGAATATTGCACATGAATTTATTGACTTGAATCCCAAGTTATTTCCCCTCTTTTTTTTCTTTTTTTTTTGCTTTTCAATTCATATAGAAGGCAAAACATATATATTTGAAAACTACTCAATTTTGGCAACCTTAATTAGACACTCACTTATGTATATACACTTTTGTGTTATTTAATCTTTATAGATAACATTAGATAAAAACAAAACAAAAAGAAAGACAAACAATTTATGAGATGCATATGGTGATGATGTGATGAAAAAGTGTAAAAAGAAAATTAGAAAAAGAAAAAAAAATTGTATGATAGGGTGTACAAAGAAATGAAGGCAGAGAAATGGTATGAATATGATGGTGATGTGATATATATTTATTCAAATTTATTGTCATTGGTGTATGCTGGAAAATTAGGAATGGAAATGAGTCAACCGTCATTGGGTGAGACACAACAAAGGTTACTTCCAGCTATGGATGAAATGAACATTGATGGAAACAATTACAGAGAGAAGAAGAAGAGATTAACAAGTAACCAGATTGATTCACTGGAGAAGAGTTTCCAAGAAGAAATAAAGTTAGACCCTGAAAGGAAGATGAAGCTTTCAAGAGAGCTAGGGCTCCAACCTCGCCAAATTGCTGTTTGGTTCCAAAATAGGCGTACAAGGTGGAAGGCTAAGCAACTTGAACACTTGTATGATGTTCTCAAACAAGAATTTGATGTTGTCTCCAAGGAAAAACAGAAACTCCAAGAAGAGGTACCTAATTATCTTTGGATTTTTTGTATAGGCTAGGATGAATTTGAATGAAATGAGATATATAACATCTATTTTTTATTATGTAACTATAATCTCACGTATTTGTTCATGTATCTCTCCCTCCATGTACCTCTTGCATTATGTGATTTTTAAGCCTATAAAAAGAAGTTGAATTATCAATAAATATTAGAGACCAAAAAAAAAAAAACATAAAATTTGTATCTAAATTGAAGATTAGTTTTTTGTCTCTAATTTCTATCAAAAATTCAACTTTTTTCCTAATAGGCGTAAGTCTGATTTTTTGTTTGAACTATGTATATGTTATGCTTTGCCTTTAGAAGCTTAGAGACCTATTACATTTCAAGTTTGAGGATAATGTTCATCTTTCTTTTGTGTTGATTTTTGCCAAAAAAAGTAAAAAAATAAATATACACACACACACAAACAACATATCACTTCTTATAATCAATACCATTTGTTAGAGTTGTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATGTCATACAAATGCAATTATACATGAGAGTGAAAAATATATTGTGTAATTAATTAATATTTTCCTTACAAAAATTACTATTTTTCTTATTTTATTTTTCCTTACACATATTCTACATTATATATATGTTTTACACTTTGTTGTATTTAAAAATATAAAGAATTATGTGCTTTTTTCTATTTCGGGGATTAACTGTATAAAAAAATTAATTATGGACATGGTGTTCTTCAACCTAGTATTACCCTACAATGGTTGATTTATGTAGCTGATCAATGTGCTCTGA
Protein sequence

>MS.gene007333.t1

MDWNESTRPFVPHTESSLSFFYNYNNTPYSGMEMSQPSLGETQQRLLPAMDEMNIDGNNYREKKKRLTSNQIDSLEKSFQEEIKLDPERKMKLSRELGLQPRQIAVWFQNRRTRWKAKQLEHLYDVLKQEFDVVSKEKQKLQEELINVL