Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007449.t1 | XP_003625597.1 | 94 | 84 | 5 | 0 | 1 | 84 | 1 | 84 | 1.80E-28 | 134.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007449.t1 | Q7XLX6 | 80.3 | 61 | 12 | 0 | 24 | 84 | 20 | 80 | 4.9e-14 | 78.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007449.t1 | G7L1S4 | 94.0 | 84 | 5 | 0 | 1 | 84 | 1 | 84 | 1.3e-28 | 134.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene007449.t1 | TF | S1Fa-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007449.t1 | MTR_7g100880 | 92.857 | 84 | 6 | 0 | 1 | 84 | 1 | 84 | 1.31e-29 | 100 |
| MS.gene007449.t1 | MTR_7g100905 | 89.474 | 76 | 3 | 1 | 9 | 84 | 3 | 73 | 2.02e-16 | 67.4 |
| MS.gene007449.t1 | MTR_7g100905 | 94.030 | 67 | 4 | 0 | 18 | 84 | 2 | 68 | 5.59e-15 | 63.5 |
| MS.gene007449.t1 | MTR_7g100890 | 82.716 | 81 | 10 | 2 | 8 | 84 | 417 | 497 | 1.04e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007449.t1 | AT2G37120 | 78.689 | 61 | 13 | 0 | 24 | 84 | 16 | 76 | 5.80e-15 | 63.5 |
| MS.gene007449.t1 | AT3G53370 | 80.000 | 60 | 12 | 0 | 25 | 84 | 17 | 76 | 7.23e-13 | 58.2 |
Find 25 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAATAGTTCTCCTGGTTAT+TGG | 0.281375 | 7.3:+12104607 | MS.gene007449:CDS |
| GTTTAGAAAGCTGGATCTCT+AGG | 0.416187 | 7.3:+12104571 | MS.gene007449:intron |
| TGGGGAAATGTTTAGAAAGC+TGG | 0.417081 | 7.3:+12104562 | MS.gene007449:intron |
| GTCTCTGCACCTGGAGAGTA+GGG | 0.431630 | 7.3:+12104757 | MS.gene007449:CDS |
| GTCCATTGCCCCTACTCTCC+AGG | 0.446840 | 7.3:-12104766 | None:intergenic |
| ATGGCCGATGACTTTGAATT+CGG | 0.448197 | 7.3:+12104223 | MS.gene007449:CDS |
| ACCAGGAGAACTATTAAGCC+TGG | 0.450176 | 7.3:-12104601 | None:intergenic |
| TTGCTGTTGACATTCCTCAT+AGG | 0.463310 | 7.3:+12104634 | MS.gene007449:CDS |
| CCCAGGCTTAATAGTTCTCC+TGG | 0.470539 | 7.3:+12104600 | MS.gene007449:CDS |
| ATAGTTCTCCTGGTTATTGG+TGG | 0.478741 | 7.3:+12104610 | MS.gene007449:CDS |
| TCCTCCTTCCTTTGATCGCA+TGG | 0.481901 | 7.3:+12104255 | MS.gene007449:CDS |
| TAGTTCTCCTGGTTATTGGT+GGG | 0.495632 | 7.3:+12104611 | MS.gene007449:CDS |
| AAGCAAGGAGTCTCTGCACC+TGG | 0.523372 | 7.3:+12104748 | MS.gene007449:CDS |
| AGTCTCTGCACCTGGAGAGT+AGG | 0.526480 | 7.3:+12104756 | MS.gene007449:CDS |
| GAAGAGTACCATGCGATCAA+AGG | 0.548062 | 7.3:-12104263 | None:intergenic |
| TTTAGAAAGCTGGATCTCTA+GGG | 0.566106 | 7.3:+12104572 | MS.gene007449:intron |
| GAATTCAAAGTCATCGGCCA+TGG | 0.568004 | 7.3:-12104221 | None:intergenic |
| AGAGTGCATAATTTCCTATG+AGG | 0.578802 | 7.3:-12104648 | None:intergenic |
| ATCACCGAATTCAAAGTCAT+CGG | 0.579370 | 7.3:-12104227 | None:intergenic |
| CCAGGAGAACTATTAAGCCT+GGG | 0.597197 | 7.3:-12104600 | None:intergenic |
| ACAGCAACCCACCAATAACC+AGG | 0.598951 | 7.3:-12104618 | None:intergenic |
| AGTACCATGCGATCAAAGGA+AGG | 0.619049 | 7.3:-12104259 | None:intergenic |
| GGATCTCTAGGGTTCAACCC+AGG | 0.683092 | 7.3:+12104583 | MS.gene007449:CDS |
| ACCATGCGATCAAAGGAAGG+AGG | 0.683503 | 7.3:-12104256 | None:intergenic |
| TCTCTGCACCTGGAGAGTAG+GGG | 0.689358 | 7.3:+12104758 | MS.gene007449:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAGTTTAACTATATGTGT+TGG | - | chr7.3:12104408-12104427 | None:intergenic | 20.0% |
| ! | ATAACACAGAGCAATTAAAT+CGG | - | chr7.3:12104512-12104531 | None:intergenic | 25.0% |
| ! | ATGAGATGTAAGAAAAGAAA+AGG | - | chr7.3:12104288-12104307 | None:intergenic | 25.0% |
| ! | CTCAAAAAAGAAGATGAAAA+AGG | + | chr7.3:12104717-12104736 | MS.gene007449:CDS | 25.0% |
| ! | TTCAAATTATTATGCTAGCT+CGG | + | chr7.3:12104440-12104459 | MS.gene007449:intron | 25.0% |
| !! | TTAATCAAGTTTTTGCTCTT+GGG | + | chr7.3:12104543-12104562 | MS.gene007449:intron | 25.0% |
| CAAGAGCAAAAACTTGATTA+AGG | - | chr7.3:12104544-12104563 | None:intergenic | 30.0% | |
| TCTTTAGATTAGACATGTCT+TGG | + | chr7.3:12104356-12104375 | MS.gene007449:intron | 30.0% | |
| TTAATAGTTCTCCTGGTTAT+TGG | + | chr7.3:12104607-12104626 | MS.gene007449:CDS | 30.0% | |
| ! | CTTAATCAAGTTTTTGCTCT+TGG | + | chr7.3:12104542-12104561 | MS.gene007449:intron | 30.0% |
| ! | TAATCAAGTTTTTGCTCTTG+GGG | + | chr7.3:12104544-12104563 | MS.gene007449:intron | 30.0% |
| ! | TTAACTATATGTGTTGGTGT+CGG | - | chr7.3:12104402-12104421 | None:intergenic | 30.0% |
| !!! | TTCATCTTCTTTTTTGAGAC+TGG | - | chr7.3:12104715-12104734 | None:intergenic | 30.0% |
| AGAGTGCATAATTTCCTATG+AGG | - | chr7.3:12104651-12104670 | None:intergenic | 35.0% | |
| ATCACCGAATTCAAAGTCAT+CGG | - | chr7.3:12104230-12104249 | None:intergenic | 35.0% | |
| TTTAGAAAGCTGGATCTCTA+GGG | + | chr7.3:12104572-12104591 | MS.gene007449:intron | 35.0% | |
| AAAAAGGAGAGACTGAAGCA+AGG | + | chr7.3:12104733-12104752 | MS.gene007449:CDS | 40.0% | |
| ATAGTTCTCCTGGTTATTGG+TGG | + | chr7.3:12104610-12104629 | MS.gene007449:CDS | 40.0% | |
| ATGGCCGATGACTTTGAATT+CGG | + | chr7.3:12104223-12104242 | MS.gene007449:CDS | 40.0% | |
| GTTTAGAAAGCTGGATCTCT+AGG | + | chr7.3:12104571-12104590 | MS.gene007449:intron | 40.0% | |
| TAGTTCTCCTGGTTATTGGT+GGG | + | chr7.3:12104611-12104630 | MS.gene007449:CDS | 40.0% | |
| TGGGGAAATGTTTAGAAAGC+TGG | + | chr7.3:12104562-12104581 | MS.gene007449:intron | 40.0% | |
| ! | ATTATTATGCTAGCTCGGTG+TGG | + | chr7.3:12104445-12104464 | MS.gene007449:intron | 40.0% |
| ! | GATTAGACATGTCTTGGTGT+AGG | + | chr7.3:12104362-12104381 | MS.gene007449:intron | 40.0% |
| ! | TTGCTGTTGACATTCCTCAT+AGG | + | chr7.3:12104634-12104653 | MS.gene007449:CDS | 40.0% |
| !!! | ACTGGCTTCTTTTTTCTAGG+AGG | - | chr7.3:12104697-12104716 | None:intergenic | 40.0% |
| !!! | CTGGCTTCTTTTTTCTAGGA+GGG | - | chr7.3:12104696-12104715 | None:intergenic | 40.0% |
| !!! | CTTCTTTTTTCTAGGAGGGA+GGG | - | chr7.3:12104692-12104711 | None:intergenic | 40.0% |
| !!! | GAGACTGGCTTCTTTTTTCT+AGG | - | chr7.3:12104700-12104719 | None:intergenic | 40.0% |
| ACCAGGAGAACTATTAAGCC+TGG | - | chr7.3:12104604-12104623 | None:intergenic | 45.0% | |
| AGTACCATGCGATCAAAGGA+AGG | - | chr7.3:12104262-12104281 | None:intergenic | 45.0% | |
| CCAGGAGAACTATTAAGCCT+GGG | - | chr7.3:12104603-12104622 | None:intergenic | 45.0% | |
| !! | CTATATGTGTTGGTGTCGGA+CGG | - | chr7.3:12104398-12104417 | None:intergenic | 45.0% |
| !! | GAAGAGTACCATGCGATCAA+AGG | - | chr7.3:12104266-12104285 | None:intergenic | 45.0% |
| !!! | GCTTCTTTTTTCTAGGAGGG+AGG | - | chr7.3:12104693-12104712 | None:intergenic | 45.0% |
| ACAGCAACCCACCAATAACC+AGG | - | chr7.3:12104621-12104640 | None:intergenic | 50.0% | |
| ACCATGCGATCAAAGGAAGG+AGG | - | chr7.3:12104259-12104278 | None:intergenic | 50.0% | |
| CCCAGGCTTAATAGTTCTCC+TGG | + | chr7.3:12104600-12104619 | MS.gene007449:CDS | 50.0% | |
| TCCTCCTTCCTTTGATCGCA+TGG | + | chr7.3:12104255-12104274 | MS.gene007449:CDS | 50.0% | |
| !! | AAGAGTGTCTGCGTGTGAGA+TGG | - | chr7.3:12104340-12104359 | None:intergenic | 50.0% |
| AAGCAAGGAGTCTCTGCACC+TGG | + | chr7.3:12104748-12104767 | MS.gene007449:CDS | 55.0% | |
| !! | GGATCTCTAGGGTTCAACCC+AGG | + | chr7.3:12104583-12104602 | MS.gene007449:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 12104223 | 12104777 | 12104223 | ID=MS.gene007449 |
| chr7.3 | mRNA | 12104223 | 12104777 | 12104223 | ID=MS.gene007449.t1;Parent=MS.gene007449 |
| chr7.3 | exon | 12104223 | 12104276 | 12104223 | ID=MS.gene007449.t1.exon1;Parent=MS.gene007449.t1 |
| chr7.3 | CDS | 12104223 | 12104276 | 12104223 | ID=cds.MS.gene007449.t1;Parent=MS.gene007449.t1 |
| chr7.3 | exon | 12104577 | 12104777 | 12104577 | ID=MS.gene007449.t1.exon2;Parent=MS.gene007449.t1 |
| chr7.3 | CDS | 12104577 | 12104777 | 12104577 | ID=cds.MS.gene007449.t1;Parent=MS.gene007449.t1 |
| Gene Sequence |
| Protein sequence |
