AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007520.t1 MTR_7g101280 94.761 649 31 2 1 647 1 648 0.0 1286
MS.gene007520.t1 MTR_0041s0110 94.761 649 31 2 1 647 1 648 0.0 1286
MS.gene007520.t1 MTR_1g064430 51.458 686 238 16 22 627 31 701 0.0 620
MS.gene007520.t1 MTR_1g064430 51.458 686 238 16 22 627 18 688 0.0 619
MS.gene007520.t1 MTR_0041s0120 82.102 352 29 1 40 391 1 318 0.0 607
MS.gene007520.t1 MTR_0041s0120 81.818 110 18 2 489 598 310 417 9.45e-51 182
MS.gene007520.t1 MTR_7g101275 76.705 352 36 1 40 391 1 306 0.0 557
MS.gene007520.t1 MTR_7g101275 81.818 110 18 2 489 598 298 405 5.79e-51 182
MS.gene007520.t1 MTR_2g094570 52.321 560 230 12 11 555 44 581 0.0 546
MS.gene007520.t1 MTR_2g094570 52.834 547 224 11 21 555 1 525 0.0 543
MS.gene007520.t1 MTR_2g094570 52.834 547 224 11 21 555 1 525 0.0 541
MS.gene007520.t1 MTR_4g058930 54.461 538 226 10 28 555 9 537 0.0 537
MS.gene007520.t1 MTR_4g058930 54.461 538 226 10 28 555 9 537 0.0 537
MS.gene007520.t1 MTR_1g094960 49.313 582 232 17 22 566 10 565 4.90e-168 494
MS.gene007520.t1 MTR_3g073420 41.825 526 252 18 27 537 15 501 9.04e-115 356
MS.gene007520.t1 MTR_5g061220 39.571 513 230 17 26 513 14 471 5.15e-98 310
MS.gene007520.t1 MTR_5g040880 33.333 573 255 18 26 556 6 493 2.38e-82 269
MS.gene007520.t1 MTR_2g024430 37.709 419 172 12 28 430 17 362 3.92e-79 260
MS.gene007520.t1 MTR_5g082140 36.219 439 204 10 26 452 7 381 7.87e-77 253
MS.gene007520.t1 MTR_1g024025 33.693 463 229 12 24 462 36 444 8.27e-70 247
MS.gene007520.t1 MTR_4g088210 38.422 393 179 11 27 406 34 376 7.51e-69 238
MS.gene007520.t1 MTR_4g088210 38.422 393 179 11 27 406 34 376 1.11e-68 237
MS.gene007520.t1 MTR_1g058210 32.877 438 226 9 26 455 7 384 5.00e-68 229
MS.gene007520.t1 MTR_2g005240 36.000 425 213 10 23 440 15 387 4.48e-67 232
MS.gene007520.t1 MTR_2g018690 35.646 418 209 11 26 434 18 384 4.32e-66 233
MS.gene007520.t1 MTR_2g093740 35.680 412 201 10 31 427 56 418 5.30e-65 227
MS.gene007520.t1 MTR_7g062540 35.507 414 203 12 31 434 14 373 9.47e-65 226
MS.gene007520.t1 MTR_8g100050 36.232 414 202 12 31 435 59 419 1.21e-64 227
MS.gene007520.t1 MTR_2g093740 35.680 412 201 10 31 427 56 418 2.16e-64 226
MS.gene007520.t1 MTR_2g093740 35.437 412 202 10 31 427 56 418 3.60e-64 227
MS.gene007520.t1 MTR_5g076270 35.589 399 198 10 26 415 19 367 4.61e-64 227
MS.gene007520.t1 MTR_8g100050 36.232 414 202 12 31 435 59 419 4.92e-64 227
MS.gene007520.t1 MTR_3g064050 35.750 400 196 11 26 415 21 369 2.02e-63 225
MS.gene007520.t1 MTR_2g006270 34.709 412 196 15 28 430 10 357 3.12e-63 216
MS.gene007520.t1 MTR_8g079492 35.407 418 209 12 26 434 18 383 5.33e-63 225
MS.gene007520.t1 MTR_8g079492 35.407 418 209 12 26 434 18 383 5.93e-63 225
MS.gene007520.t1 MTR_4g124900 33.726 424 212 11 26 438 18 383 1.22e-61 223
MS.gene007520.t1 MTR_4g124900 33.726 424 212 11 26 438 18 383 1.30e-61 222
MS.gene007520.t1 MTR_2g093740 35.059 425 199 11 31 427 56 431 1.93e-61 218
MS.gene007520.t1 MTR_4g060460 33.937 442 225 10 31 454 54 446 5.15e-61 218
MS.gene007520.t1 MTR_5g040740 34.913 401 173 12 28 414 9 335 5.45e-61 209
MS.gene007520.t1 MTR_2g093740 34.588 425 201 11 31 427 56 431 8.66e-61 216
MS.gene007520.t1 MTR_2g093740 34.353 425 202 11 31 427 56 431 1.35e-60 217
MS.gene007520.t1 MTR_4g060460 35.280 411 199 10 31 425 54 413 1.95e-60 216
MS.gene007520.t1 MTR_2g043250 36.029 408 198 10 13 408 1 357 2.33e-60 219
MS.gene007520.t1 MTR_8g027440 35.280 411 204 10 25 427 23 379 1.59e-59 212
MS.gene007520.t1 MTR_8g101360 47.222 216 102 3 28 235 28 239 1.31e-56 207
MS.gene007520.t1 MTR_8g101360 38.000 100 59 3 312 409 270 368 2.42e-11 67.8
MS.gene007520.t1 MTR_4g021580 34.135 416 211 11 29 434 17 379 1.67e-56 202
MS.gene007520.t1 MTR_4g021580 34.135 416 211 11 29 434 17 379 2.04e-56 203
MS.gene007520.t1 MTR_2g014770 46.262 214 104 3 27 232 49 259 2.09e-51 188
MS.gene007520.t1 MTR_2g014770 35.455 110 66 3 298 406 282 387 3.58e-14 76.3
MS.gene007520.t1 MTR_2g014770 46.262 214 104 3 27 232 49 259 6.00e-51 187
MS.gene007520.t1 MTR_2g014770 35.455 110 66 3 298 406 282 387 3.20e-14 76.6
MS.gene007520.t1 MTR_8g079492 35.328 351 171 10 88 434 3 301 1.68e-49 185
MS.gene007520.t1 MTR_5g061890 31.671 401 191 13 28 413 9 341 6.11e-49 176
MS.gene007520.t1 MTR_5g060780 31.139 395 190 12 28 407 9 336 3.04e-48 174
MS.gene007520.t1 MTR_2g006380 31.961 413 199 16 28 430 17 357 4.85e-48 175
MS.gene007520.t1 MTR_5g460920 31.139 395 190 12 28 407 9 336 1.23e-47 172
MS.gene007520.t1 MTR_2g093740 37.124 299 138 6 131 427 38 288 1.18e-46 174
MS.gene007520.t1 MTR_2g093740 37.124 299 138 6 131 427 38 288 1.40e-46 175
MS.gene007520.t1 MTR_2g093740 35.577 312 138 7 131 427 38 301 3.04e-43 165
MS.gene007520.t1 MTR_2g093740 35.577 312 138 7 131 427 38 301 3.14e-43 166
MS.gene007520.t1 MTR_5g060630 28.107 523 248 18 26 476 7 473 1.60e-42 161
MS.gene007520.t1 MTR_5g460740 28.107 523 248 18 26 476 7 473 1.60e-42 161
MS.gene007520.t1 MTR_8g446900 29.974 387 199 13 73 454 8 327 3.29e-39 149
MS.gene007520.t1 MTR_5g062970 35.644 202 112 3 27 219 34 226 3.63e-37 139
MS.gene007520.t1 MTR_5g062960 33.889 180 92 6 301 476 1 157 3.31e-22 95.5
MS.gene007520.t1 MTR_5g060770 28.947 190 110 8 76 254 6 181 2.65e-13 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007520.t1 AT4G30080 54.737 475 182 12 26 487 14 468 7.01e-167 493
MS.gene007520.t1 AT2G28350 46.777 605 263 16 26 600 5 580 5.25e-163 483
MS.gene007520.t1 AT2G28350 46.777 605 263 16 26 600 5 580 1.15e-162 483
MS.gene007520.t1 AT1G77850 38.043 552 304 10 25 563 13 539 2.52e-113 352
MS.gene007520.t1 AT1G77850 38.043 552 304 10 25 563 13 539 3.31e-113 352
MS.gene007520.t1 AT1G19850 35.784 408 193 11 15 406 23 377 2.35e-68 239
MS.gene007520.t1 AT1G19850 35.784 408 193 11 15 406 34 388 2.63e-68 239
MS.gene007520.t1 AT1G30330 35.520 442 221 12 1 434 1 386 4.56e-68 239
MS.gene007520.t1 AT1G30330 35.520 442 221 12 1 434 1 386 4.56e-68 239
MS.gene007520.t1 AT1G59750 34.357 521 273 17 31 536 22 488 2.43e-67 233
MS.gene007520.t1 AT1G59750 34.357 521 273 17 31 536 22 488 2.43e-67 233
MS.gene007520.t1 AT1G59750 34.489 519 270 16 31 536 22 483 2.85e-67 233
MS.gene007520.t1 AT1G30330 36.211 417 208 11 26 434 18 384 3.49e-67 237
MS.gene007520.t1 AT1G59750 34.674 522 267 18 31 536 22 485 6.70e-67 231
MS.gene007520.t1 AT5G62000 35.160 438 217 11 2 425 26 410 1.22e-65 231
MS.gene007520.t1 AT5G62000 35.160 438 217 11 2 425 26 410 1.22e-65 231
MS.gene007520.t1 AT5G62000 35.160 438 217 11 2 425 26 410 1.22e-65 231
MS.gene007520.t1 AT5G62000 35.160 438 217 11 2 425 26 410 1.22e-65 231
MS.gene007520.t1 AT5G62000 35.160 438 217 11 2 425 26 410 1.33e-65 231
MS.gene007520.t1 AT5G60450 34.532 417 210 10 22 424 32 399 3.20e-65 229
MS.gene007520.t1 AT5G37020 35.500 400 197 11 26 415 17 365 3.23e-65 229
MS.gene007520.t1 AT5G37020 35.500 400 197 11 26 415 17 365 7.01e-65 229
MS.gene007520.t1 AT5G60450 34.804 408 203 10 31 424 65 423 1.54e-64 228
MS.gene007520.t1 AT2G33860 32.974 464 239 14 31 475 54 464 7.04e-64 222
MS.gene007520.t1 AT3G61830 33.875 431 214 12 31 451 25 394 1.86e-60 213
MS.gene007520.t1 AT2G46530 34.456 386 187 10 31 406 21 350 1.01e-59 211
MS.gene007520.t1 AT2G46530 34.456 386 187 10 31 406 42 371 1.60e-59 211
MS.gene007520.t1 AT1G34310 34.815 405 203 9 28 427 24 372 4.13e-58 206
MS.gene007520.t1 AT1G35520 34.230 409 206 9 31 434 27 377 2.70e-57 204
MS.gene007520.t1 AT1G35240 33.902 410 206 10 31 434 27 377 3.55e-57 203
MS.gene007520.t1 AT1G34410 34.063 411 204 10 31 434 27 377 1.19e-55 199
MS.gene007520.t1 AT1G35540 33.835 399 204 9 31 424 27 370 1.15e-54 197
MS.gene007520.t1 AT1G19220 45.622 217 107 3 26 235 18 230 3.88e-54 200
MS.gene007520.t1 AT1G19220 36.220 127 78 3 312 437 261 385 7.25e-14 75.9
MS.gene007520.t1 AT5G20730 43.318 217 112 3 26 235 19 231 1.86e-52 196
MS.gene007520.t1 AT5G20730 34.375 128 80 4 312 437 262 387 5.94e-12 69.7
MS.gene007520.t1 AT5G20730 43.318 217 112 3 26 235 19 231 1.96e-52 195
MS.gene007520.t1 AT5G20730 33.858 127 81 3 312 437 262 386 7.05e-13 72.8
MS.gene007520.t1 AT5G20730 43.318 217 112 3 26 235 19 231 2.08e-52 195
MS.gene007520.t1 AT5G20730 34.375 128 80 4 312 437 262 387 5.24e-12 69.7
MS.gene007520.t1 AT4G23980 31.658 398 211 10 31 420 12 356 8.30e-51 187
MS.gene007520.t1 AT4G23980 31.658 398 211 10 31 420 12 356 1.12e-50 186
MS.gene007520.t1 AT1G34170 32.187 407 210 10 9 406 3 352 6.10e-50 182
MS.gene007520.t1 AT3G61830 34.174 357 170 10 73 425 22 317 1.10e-49 182
MS.gene007520.t1 AT1G34390 32.266 406 204 10 31 427 27 370 3.50e-47 176
MS.gene007520.t1 AT5G37020 36.934 287 132 7 131 415 31 270 5.70e-47 177
MS.gene007520.t1 AT2G33860 33.427 356 176 11 131 475 2 307 1.38e-46 171
MS.gene007520.t1 AT1G34170 30.864 405 192 10 9 406 3 326 1.42e-43 163
MS.gene007520.t1 AT1G34170 30.958 407 189 10 9 406 3 326 4.48e-43 163
MS.gene007520.t1 AT2G46530 34.173 278 132 6 130 406 36 263 3.16e-41 157
MS.gene007520.t1 AT1G43950 38.537 205 112 6 31 230 27 222 9.74e-35 131
MS.gene007520.t1 AT3G25730 26.738 187 108 4 82 254 122 293 5.72e-12 67.8
MS.gene007520.t1 AT2G46870 37.113 97 60 1 123 219 27 122 8.17e-12 67.4
MS.gene007520.t1 AT1G13260 30.147 136 76 3 118 251 175 293 7.54e-11 64.7

Find 156 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAAGTTGGCATTGGAAAT+TGG 0.228346 7.4:-9198427 None:intergenic
TGGAATGAAAGACGGAATTT+TGG 0.237258 7.4:-9196718 None:intergenic
CATCAACGTGTCTGAAATTA+AGG 0.241218 7.4:+9196860 MS.gene007520:CDS
TGGCCATCAATTGGGAAATC+TGG 0.249420 7.4:-9198407 None:intergenic
GGCATTTCAGGCACGTTTAT+AGG 0.250063 7.4:+9197048 MS.gene007520:CDS
GCATTTCAGGCACGTTTATA+GGG 0.272489 7.4:+9197049 MS.gene007520:CDS
CTTGTGAAAACTTCTTTAAT+TGG 0.278075 7.4:+9197485 MS.gene007520:CDS
CAGCTATGGCATGCCGTTGC+AGG 0.280375 7.4:+9196597 MS.gene007520:CDS
ATGGCGAAAAGTGGCATTTC+AGG 0.309403 7.4:+9197036 MS.gene007520:CDS
CGGAACTATTGCTTCTGTTC+AGG 0.311558 7.4:+9197595 MS.gene007520:CDS
GTTGGAGTCGATCCTTTGTT+AGG 0.313766 7.4:+9197218 MS.gene007520:CDS
TTCCACTAGCGGCGCGAAAA+TGG 0.315002 7.4:+9199072 MS.gene007520:CDS
CTCCCAGATTTCCCAATTGA+TGG 0.331554 7.4:+9198404 MS.gene007520:CDS
TTAGGATTTAGAAGGTTGTT+TGG 0.339574 7.4:-9198449 None:intergenic
TTCAGCTAACCCTCCTCTTC+AGG 0.348707 7.4:+9196992 MS.gene007520:CDS
TTGGAATATTAGGATTTAGA+AGG 0.349891 7.4:-9198457 None:intergenic
TTGGAATTCGGAGGGCTAAA+AGG 0.350812 7.4:+9197186 MS.gene007520:CDS
TATTATTATTAAACGGTTGT+TGG 0.355810 7.4:-9198595 None:intergenic
ATTGGAAATTGGCCATCAAT+TGG 0.356580 7.4:-9198416 None:intergenic
CATGCCGGCAGGACTAGTTT+CGG 0.360765 7.4:-9198492 None:intergenic
AGAAGGTTGTTTGGAAAAGT+TGG 0.365277 7.4:-9198440 None:intergenic
TGGAAATCTGGTAGTGGAAT+TGG 0.365957 7.4:+9197242 MS.gene007520:CDS
CTGCGAATGTTTCTTCGCAT+TGG 0.366575 7.4:-9198898 None:intergenic
CAGTGTCGACTTGTATTGTT+TGG 0.368403 7.4:-9198769 None:intergenic
AATGGAAGATTATGAAAATC+TGG 0.372719 7.4:-9198551 None:intergenic
TTTGCAATGCCAAGTTATTA+GGG 0.373972 7.4:-9198640 None:intergenic
TCAAGGATAAGTTGGTTTAT+CGG 0.374081 7.4:+9197575 MS.gene007520:CDS
GGAGCCAGGCATGGTCATTT+CGG 0.375741 7.4:+9198518 MS.gene007520:CDS
TTTGATAATGATGGTGTTGC+TGG 0.376636 7.4:+9196837 MS.gene007520:CDS
CTAACTTGGTTAATATTCAT+AGG 0.385591 7.4:-9196813 None:intergenic
AAGAGGCATTTGTTGACAAC+TGG 0.392584 7.4:+9197080 MS.gene007520:CDS
CCTTTGTTAGGGTGGAAATC+TGG 0.395570 7.4:+9197230 MS.gene007520:CDS
GGTAATCTCAATTTCTTCCT+TGG 0.395833 7.4:-9198377 None:intergenic
TTGGAACAGCACTTCAGATT+AGG 0.401029 7.4:+9197504 MS.gene007520:CDS
GCGATCTTCATGTTGGAATT+CGG 0.404653 7.4:+9197174 MS.gene007520:CDS
CTGGCTCCCTGCATGCCGGC+AGG 0.407927 7.4:-9198503 None:intergenic
GCCATCTTGAACCTCATTCC+AGG 0.409391 7.4:-9197533 None:intergenic
TTAAACGGTTGTTGGAAACT+AGG 0.416552 7.4:-9198587 None:intergenic
AACTAGTCCTGCCGGCATGC+AGG 0.419630 7.4:+9198496 MS.gene007520:CDS
CAATCCAACTGAGATGAATA+TGG 0.421601 7.4:+9196530 MS.gene007520:CDS
AAAACATGTGAGTTAACTTC+TGG 0.421701 7.4:-9196636 None:intergenic
AGCGACAAGAAACTTGCGTC+TGG 0.427650 7.4:+9197119 MS.gene007520:CDS
TTGGAAATTGGCCATCAATT+GGG 0.428714 7.4:-9198415 None:intergenic
TGCACAATCTTCAGAGAAAC+CGG 0.428735 7.4:+9198712 MS.gene007520:CDS
GTCGCAATATTATTATTAAA+CGG 0.430671 7.4:-9198602 None:intergenic
CTTATTGCTTCAACTCTACA+TGG 0.431451 7.4:-9196738 None:intergenic
CTGCCGGCATGCAGGGAGCC+AGG 0.432868 7.4:+9198504 MS.gene007520:CDS
ACATTAGAGATGAAACATTC+AGG 0.433701 7.4:+9198926 MS.gene007520:CDS
GAAATGTTCTCACTGGTGTT+CGG 0.434122 7.4:-9198665 None:intergenic
AGTTGAAGCAATAAGATATA+TGG 0.445009 7.4:+9196746 MS.gene007520:CDS
CTAGGCTGAAAGAGACCTAA+TGG 0.448362 7.4:-9198569 None:intergenic
CATCATTATCAAAACTAACT+TGG 0.449861 7.4:-9196827 None:intergenic
TGATGAGTCCTCAGTTTCAA+TGG 0.449908 7.4:-9197555 None:intergenic
GATAATAAACTTCGAAGAAA+CGG 0.452871 7.4:+9197317 MS.gene007520:CDS
GCTAATAAGTGATGGAATGA+TGG 0.454476 7.4:+9197355 MS.gene007520:CDS
GTTGTCAACAAATGCCTCTT+CGG 0.456311 7.4:-9197077 None:intergenic
ATGCCGGCAGGACTAGTTTC+GGG 0.456648 7.4:-9198491 None:intergenic
TTCTTCCTTGGCGGTATGAA+AGG 0.458131 7.4:-9198365 None:intergenic
CGCCGAGCATGCATGTGAAC+CGG 0.459270 7.4:+9196677 MS.gene007520:CDS
AGTTTCTTGTCGCTGACAAA+AGG 0.461470 7.4:-9197110 None:intergenic
GTGGTTTATTATCCTCGGTC+AGG 0.470650 7.4:+9197449 MS.gene007520:CDS
CACCGGTTCACATGCATGCT+CGG 0.471821 7.4:-9196679 None:intergenic
AAGTGATGGAATGATGGGTA+GGG 0.472687 7.4:+9197361 MS.gene007520:CDS
GACTCCATATTCATCTCAGT+TGG 0.472803 7.4:-9196534 None:intergenic
TTGGAGTCGATCCTTTGTTA+GGG 0.473989 7.4:+9197219 MS.gene007520:CDS
ATTTGTCCTGCACCACCTTA+CGG 0.476461 7.4:+9197266 MS.gene007520:CDS
CGAAGAAACGGTAAAGGTAA+TGG 0.476494 7.4:+9197329 MS.gene007520:CDS
ATCCAGCTCTACAATTGAAC+CGG 0.480648 7.4:-9199117 None:intergenic
CACCGGTTCAATTGTAGAGC+TGG 0.485302 7.4:+9199115 MS.gene007520:CDS
TTGATGTGGTTTATTATCCT+CGG 0.489017 7.4:+9197444 MS.gene007520:CDS
TTGTTTGGAAAAGTTGGCAT+TGG 0.489751 7.4:-9198434 None:intergenic
GGATAATAAACCACATCAAA+CGG 0.491751 7.4:-9197440 None:intergenic
GGCCATCAATTGGGAAATCT+GGG 0.492331 7.4:-9198406 None:intergenic
AGGACTCATCAAGGATAAGT+TGG 0.493550 7.4:+9197567 MS.gene007520:CDS
CTTCAGATTAGGTGGTGTCC+TGG 0.494054 7.4:+9197515 MS.gene007520:CDS
TGGTGCGGCTTTGCATGATC+CGG 0.495348 7.4:-9198731 None:intergenic
AATCTCAATTTCTTCCTTGG+CGG 0.497432 7.4:-9198374 None:intergenic
CATCAGATGTACGTGTCGGC+AGG 0.505339 7.4:-9198850 None:intergenic
TAAGTGATGGAATGATGGGT+AGG 0.517866 7.4:+9197360 MS.gene007520:CDS
TTCAGGCTGCTGATCCGTCA+TGG 0.519149 7.4:+9197612 MS.gene007520:CDS
ACTAGTCCTGCCGGCATGCA+GGG 0.520362 7.4:+9198497 MS.gene007520:CDS
ATCTTCATGTTGGAATTCGG+AGG 0.523611 7.4:+9197177 MS.gene007520:CDS
GATGACTGTCCCTAATAACT+TGG 0.526305 7.4:+9198631 MS.gene007520:CDS
GGCATGCAGGGAGCCAGGCA+TGG 0.527776 7.4:+9198509 MS.gene007520:CDS
AAGACCGAAATGACCATGCC+TGG 0.532259 7.4:-9198522 None:intergenic
AATCCTCCGTAAGGTGGTGC+AGG 0.533772 7.4:-9197272 None:intergenic
TTAGGGTGGAAATCTGGTAG+TGG 0.537403 7.4:+9197236 MS.gene007520:CDS
AGCGTTTCTGCACAATATCT+AGG 0.540389 7.4:-9196951 None:intergenic
GCGACAAGAAACTTGCGTCT+GGG 0.540853 7.4:+9197120 MS.gene007520:CDS
GGCGGTATGAAAGGCGAAAG+AGG 0.541655 7.4:-9198356 None:intergenic
GAGTAAGCACTGAAATTCAC+CGG 0.548533 7.4:-9196696 None:intergenic
AGGATGTTCATGGCGAAAAG+TGG 0.550349 7.4:+9197027 MS.gene007520:CDS
CGACAAGAAACTTGCGTCTG+GGG 0.550603 7.4:+9197121 MS.gene007520:CDS
AATGGATTGCTAATAAGTGA+TGG 0.550837 7.4:+9197347 MS.gene007520:CDS
TTACCCGAAACTAGTCCTGC+CGG 0.553790 7.4:+9198488 MS.gene007520:CDS
AAGATTATGAAAATCTGGTA+AGG 0.555027 7.4:-9198546 None:intergenic
GCTAAAAGGCGAAATAATGT+TGG 0.555377 7.4:+9197200 MS.gene007520:CDS
ATGCCTGGCTCCCTGCATGC+CGG 0.557141 7.4:-9198507 None:intergenic
GTGATGGAATGATGGGTAGG+GGG 0.558347 7.4:+9197363 MS.gene007520:CDS
CATTTCAGGCACGTTTATAG+GGG 0.562067 7.4:+9197050 MS.gene007520:CDS
TTGCACCATTCCTCCTGCAA+CGG 0.567997 7.4:-9196610 None:intergenic
AGTGATGGAATGATGGGTAG+GGG 0.568376 7.4:+9197362 MS.gene007520:CDS
GGCATTTGTTGACAACTGGA+TGG 0.569244 7.4:+9197084 MS.gene007520:CDS
TTAGGTGGTGTCCTGGAATG+AGG 0.569864 7.4:+9197522 MS.gene007520:CDS
ATTGGGAAATCTGGGAGTTG+AGG 0.570713 7.4:-9198398 None:intergenic
TTGAAACTGAGGACTCATCA+AGG 0.572823 7.4:+9197558 MS.gene007520:CDS
AAACTTCGAAGAAACGGTAA+AGG 0.575815 7.4:+9197323 MS.gene007520:CDS
GAGACTTCATCAGAAAAGCT+AGG 0.576418 7.4:+9199029 MS.gene007520:intron
AACGATGGAAATCCTCCGTA+AGG 0.577357 7.4:-9197281 None:intergenic
GAAAACGGCGATCTTCATGT+TGG 0.577451 7.4:+9197167 MS.gene007520:CDS
AGAGTGTGGACCCTCAGCTA+TGG 0.582931 7.4:+9196583 MS.gene007520:CDS
GAGTCGATCCTTTGTTAGGG+TGG 0.585067 7.4:+9197222 MS.gene007520:CDS
CTATGGCATGCCGTTGCAGG+AGG 0.585809 7.4:+9196600 MS.gene007520:CDS
TAATATGCAGCCGTTTGATG+TGG 0.585844 7.4:+9197430 MS.gene007520:CDS
AAGTTATTAGGGACAGTCAT+CGG 0.589613 7.4:-9198629 None:intergenic
CTAATAAGTGATGGAATGAT+GGG 0.591799 7.4:+9197356 MS.gene007520:CDS
GCAACGGCATGCCATAGCTG+AGG 0.591988 7.4:-9196594 None:intergenic
GCATGCTCGGCGTGACCCTG+AGG 0.592711 7.4:-9196666 None:intergenic
CATGCTCGGCGTGACCCTGA+GGG 0.593580 7.4:-9196665 None:intergenic
CGTCATGGTCTGATTCTATG+TGG 0.597184 7.4:+9197627 MS.gene007520:CDS
GTTTGCATCAGATTGTGTCA+AGG 0.603398 7.4:-9196907 None:intergenic
CGAGCATGAAATGTTCTCAC+TGG 0.604009 7.4:-9198672 None:intergenic
GCATGCCGTTGCAGGAGGAA+TGG 0.606658 7.4:+9196605 MS.gene007520:CDS
ATGAAGTGTATGCAAAACTC+AGG 0.607388 7.4:+9196784 MS.gene007520:CDS
CAGCTCTACAATTGAACCGG+TGG 0.607879 7.4:-9199114 None:intergenic
GATACTATTTCAACTTGCCA+CGG 0.610748 7.4:-9198317 None:intergenic
GCGGTATGAAAGGCGAAAGA+GGG 0.612138 7.4:-9198355 None:intergenic
CACTAGCGGCGCGAAAATGG+AGG 0.612645 7.4:+9199075 MS.gene007520:CDS
ACTCTACATGGAATGAAAGA+CGG 0.613123 7.4:-9196726 None:intergenic
CAACGGCATGCCATAGCTGA+GGG 0.613865 7.4:-9196593 None:intergenic
TCCTGGAATGAGGTTCAAGA+TGG 0.614384 7.4:+9197532 MS.gene007520:CDS
TCTTCATGTTGGAATTCGGA+GGG 0.615735 7.4:+9197178 MS.gene007520:CDS
TTTCGCCTTTCATACCGCCA+AGG 0.619271 7.4:+9198360 MS.gene007520:CDS
TCTTCCTCTCCCGAAAACGA+TGG 0.620463 7.4:-9197296 None:intergenic
GAACAGCACTTCAGATTAGG+TGG 0.620797 7.4:+9197507 MS.gene007520:CDS
TTTATAGGGGAACGCCGAAG+AGG 0.625074 7.4:+9197063 MS.gene007520:CDS
GTTATTGAAGCAATAAAACT+CGG 0.633322 7.4:+9197404 MS.gene007520:CDS
CCAGATTTCCACCCTAACAA+AGG 0.634082 7.4:-9197230 None:intergenic
CTCTCATCAGATGTACGTGT+CGG 0.637287 7.4:-9198854 None:intergenic
CAAGATGGCCATTGAAACTG+AGG 0.643200 7.4:+9197547 MS.gene007520:CDS
AAAGGCGAAAGAGGGATCGA+AGG 0.643435 7.4:-9198347 None:intergenic
GATGGAAATCCTCCGTAAGG+TGG 0.645097 7.4:-9197278 None:intergenic
TTCTATGTGGAGACTTCTCG+AGG 0.645176 7.4:+9197640 MS.gene007520:CDS
ATGTGAAAAGAGTGAATCCG+TGG 0.650057 7.4:+9198300 MS.gene007520:CDS
CAAACAATACAAGTCGACAC+TGG 0.651728 7.4:+9198770 MS.gene007520:CDS
TCTGATGCAAACAATGGTGG+TGG 0.655667 7.4:+9196918 MS.gene007520:CDS
CAATGCGAAGAAACATTCGC+AGG 0.655730 7.4:+9198899 MS.gene007520:CDS
CATCTGATGAGAGGTTCGAG+TGG 0.680653 7.4:+9198864 MS.gene007520:CDS
TGTCCTGCACCACCTTACGG+AGG 0.685773 7.4:+9197269 MS.gene007520:CDS
CACATAGAATCAGACCATGA+CGG 0.689130 7.4:-9197626 None:intergenic
CGACACGTACATCTGATGAG+AGG 0.689516 7.4:+9198855 MS.gene007520:CDS
ACTTCATCAGAAAAGCTAGG+AGG 0.691502 7.4:+9199032 MS.gene007520:CDS
ACACAATCTGATGCAAACAA+TGG 0.696099 7.4:+9196912 MS.gene007520:CDS
CAATCTGATGCAAACAATGG+TGG 0.722659 7.4:+9196915 MS.gene007520:CDS
AACTCAGGAGTACCTGACCG+AGG 0.728041 7.4:-9197461 None:intergenic
TTAGCTGAATAGTCCAAACG+AGG 0.756003 7.4:-9196978 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAATTGGAATATT+AGG - chr7.4:9198470-9198489 None:intergenic 10.0%
!! TAGTAATATATGCAATAAAA+AGG - chr7.4:9198177-9198196 None:intergenic 15.0%
!! TTAATGTATATATTTGTGTA+TGG + chr7.4:9198060-9198079 MS.gene007520:intron 15.0%
!!! GTTAGATTTTACTTATATTT+TGG + chr7.4:9197766-9197785 MS.gene007520:intron 15.0%
!!! TTTTAGCAAATTCTAATAAT+AGG - chr7.4:9197955-9197974 None:intergenic 15.0%
!! ATTCAAAGTAAAATATGTGA+AGG - chr7.4:9198022-9198041 None:intergenic 20.0%
!! GTCGCAATATTATTATTAAA+CGG - chr7.4:9198605-9198624 None:intergenic 20.0%
!! TATTATTATTAAACGGTTGT+TGG - chr7.4:9198598-9198617 None:intergenic 20.0%
!! TCAAGTAAATTAATTCTAGA+GGG - chr7.4:9197689-9197708 None:intergenic 20.0%
!!! GAATGTTTTGAGAATAATAA+CGG + chr7.4:9197726-9197745 MS.gene007520:intron 20.0%
! AACTTAAAAGGATTATGCTT+TGG - chr7.4:9197905-9197924 None:intergenic 25.0%
! AAGATTATGAAAATCTGGTA+AGG - chr7.4:9198549-9198568 None:intergenic 25.0%
! AATGGAAGATTATGAAAATC+TGG - chr7.4:9198554-9198573 None:intergenic 25.0%
! AGTTGAAGCAATAAGATATA+TGG + chr7.4:9196746-9196765 MS.gene007520:CDS 25.0%
! CATCATTATCAAAACTAACT+TGG - chr7.4:9196830-9196849 None:intergenic 25.0%
! CTAACTTGGTTAATATTCAT+AGG - chr7.4:9196816-9196835 None:intergenic 25.0%
! CTCAAGTAAATTAATTCTAG+AGG - chr7.4:9197690-9197709 None:intergenic 25.0%
! CTTGTGAAAACTTCTTTAAT+TGG + chr7.4:9197485-9197504 MS.gene007520:CDS 25.0%
! GATAATAAACTTCGAAGAAA+CGG + chr7.4:9197317-9197336 MS.gene007520:CDS 25.0%
! GTATGGTAATATAGATACAA+TGG + chr7.4:9198077-9198096 MS.gene007520:intron 25.0%
! GTTATTGAAGCAATAAAACT+CGG + chr7.4:9197404-9197423 MS.gene007520:CDS 25.0%
! TCTCTTCTTACAATTTCATT+AGG - chr7.4:9198116-9198135 None:intergenic 25.0%
! TTGGAATATTAGGATTTAGA+AGG - chr7.4:9198460-9198479 None:intergenic 25.0%
!! GATTTTCATAATCTTCCATT+AGG + chr7.4:9198554-9198573 MS.gene007520:CDS 25.0%
!! GTTTCGGGTAAATAAAAAAT+TGG - chr7.4:9198479-9198498 None:intergenic 25.0%
!! TGATTTGTATAAATTCCACA+AGG - chr7.4:9197836-9197855 None:intergenic 25.0%
!! TTTTCATCAGTACCATAAAT+TGG + chr7.4:9197923-9197942 MS.gene007520:intron 25.0%
!!! ATGATTAGTCATTTTCTTCT+CGG - chr7.4:9198807-9198826 None:intergenic 25.0%
!!! CAAGTTAGTTTTGATAATGA+TGG + chr7.4:9196828-9196847 MS.gene007520:CDS 25.0%
!!! TCATACTTTTTAGGTTACAT+GGG + chr7.4:9198256-9198275 MS.gene007520:intron 25.0%
!!! TTCACATTTTTAAGTATCTC+AGG - chr7.4:9198287-9198306 None:intergenic 25.0%
!!! TTTCTGATGTCATACTTTTT+AGG + chr7.4:9198247-9198266 MS.gene007520:intron 25.0%
AAAACATGTGAGTTAACTTC+TGG - chr7.4:9196639-9196658 None:intergenic 30.0%
AATAGGAAGCTTCCAATTTA+TGG - chr7.4:9197938-9197957 None:intergenic 30.0%
ACATTAGAGATGAAACATTC+AGG + chr7.4:9198926-9198945 MS.gene007520:CDS 30.0%
CTACATCACTATCATACATT+AGG + chr7.4:9197984-9198003 MS.gene007520:intron 30.0%
GGATAATAAACCACATCAAA+CGG - chr7.4:9197443-9197462 None:intergenic 30.0%
TAGATACAATGGAACATTGT+TGG + chr7.4:9198088-9198107 MS.gene007520:intron 30.0%
TGACATCAGAAAATCATTCA+CGG - chr7.4:9198239-9198258 None:intergenic 30.0%
TTAGGATTTAGAAGGTTGTT+TGG - chr7.4:9198452-9198471 None:intergenic 30.0%
TTGATGTGGTTTATTATCCT+CGG + chr7.4:9197444-9197463 MS.gene007520:CDS 30.0%
TTGCAAGAATACACATTCTA+TGG - chr7.4:9197870-9197889 None:intergenic 30.0%
TTTGCAATGCCAAGTTATTA+GGG - chr7.4:9198643-9198662 None:intergenic 30.0%
! CTAATAAGTGATGGAATGAT+GGG + chr7.4:9197356-9197375 MS.gene007520:CDS 30.0%
! CTAATGTATGATAGTGATGT+AGG - chr7.4:9197986-9198005 None:intergenic 30.0%
! TAGGAGGTTGACAATTTTAA+TGG + chr7.4:9199048-9199067 MS.gene007520:CDS 30.0%
! TATGATAGTGATGTAGGTAT+AGG - chr7.4:9197980-9197999 None:intergenic 30.0%
! TTTTGCAATGCCAAGTTATT+AGG - chr7.4:9198644-9198663 None:intergenic 30.0%
!! AATGGATTGCTAATAAGTGA+TGG + chr7.4:9197347-9197366 MS.gene007520:CDS 30.0%
!! GGTACTGATGAAAACTTAAA+AGG - chr7.4:9197917-9197936 None:intergenic 30.0%
!! TCAAGGATAAGTTGGTTTAT+CGG + chr7.4:9197575-9197594 MS.gene007520:CDS 30.0%
!!! ATTTTAATGGATTCCACTAG+CGG + chr7.4:9199061-9199080 MS.gene007520:CDS 30.0%
!!! GTCATACTTTTTAGGTTACA+TGG + chr7.4:9198255-9198274 MS.gene007520:intron 30.0%
!!! GTGTTTTTGAGATCTGAAAA+CGG + chr7.4:9197152-9197171 MS.gene007520:CDS 30.0%
AAACTTCGAAGAAACGGTAA+AGG + chr7.4:9197323-9197342 MS.gene007520:CDS 35.0%
AAGTTATTAGGGACAGTCAT+CGG - chr7.4:9198632-9198651 None:intergenic 35.0%
AATCTCAATTTCTTCCTTGG+CGG - chr7.4:9198377-9198396 None:intergenic 35.0%
ACACAATCTGATGCAAACAA+TGG + chr7.4:9196912-9196931 MS.gene007520:CDS 35.0%
ACTCTACATGGAATGAAAGA+CGG - chr7.4:9196729-9196748 None:intergenic 35.0%
AGAAGGTTGTTTGGAAAAGT+TGG - chr7.4:9198443-9198462 None:intergenic 35.0%
ATCAGCAAGAAAAAAACCAC+CGG + chr7.4:9199098-9199117 MS.gene007520:CDS 35.0%
ATGAAGTGTATGCAAAACTC+AGG + chr7.4:9196784-9196803 MS.gene007520:CDS 35.0%
ATTGGAAATTGGCCATCAAT+TGG - chr7.4:9198419-9198438 None:intergenic 35.0%
CAATCCAACTGAGATGAATA+TGG + chr7.4:9196530-9196549 MS.gene007520:CDS 35.0%
CTTATTGCTTCAACTCTACA+TGG - chr7.4:9196741-9196760 None:intergenic 35.0%
GAAAAATATCCTGAAGAGGA+GGG - chr7.4:9197004-9197023 None:intergenic 35.0%
GAAAAATGTGAACAAGAGTG+TGG + chr7.4:9196569-9196588 MS.gene007520:CDS 35.0%
GATACTATTTCAACTTGCCA+CGG - chr7.4:9198320-9198339 None:intergenic 35.0%
GCTAAAAGGCGAAATAATGT+TGG + chr7.4:9197200-9197219 MS.gene007520:CDS 35.0%
GGTAATCTCAATTTCTTCCT+TGG - chr7.4:9198380-9198399 None:intergenic 35.0%
TTAAACGGTTGTTGGAAACT+AGG - chr7.4:9198590-9198609 None:intergenic 35.0%
TTGGAAATTGGCCATCAATT+GGG - chr7.4:9198418-9198437 None:intergenic 35.0%
TTGGGAAAAATATCCTGAAG+AGG - chr7.4:9197008-9197027 None:intergenic 35.0%
TTGTCATATAGCAGCTATAG+TGG + chr7.4:9197795-9197814 MS.gene007520:intron 35.0%
TTGTTTGGAAAAGTTGGCAT+TGG - chr7.4:9198437-9198456 None:intergenic 35.0%
! ATTTTTCCCAAGGATGTTCA+TGG + chr7.4:9197017-9197036 MS.gene007520:CDS 35.0%
! CATCAACGTGTCTGAAATTA+AGG + chr7.4:9196860-9196879 MS.gene007520:CDS 35.0%
! GAAAAGTTGGCATTGGAAAT+TGG - chr7.4:9198430-9198449 None:intergenic 35.0%
! GAAGTTTTCACAAGAAACTC+AGG - chr7.4:9197479-9197498 None:intergenic 35.0%
! GAGTTTTGCATACACTTCAT+CGG - chr7.4:9196784-9196803 None:intergenic 35.0%
! GCTAATAAGTGATGGAATGA+TGG + chr7.4:9197355-9197374 MS.gene007520:CDS 35.0%
! TCTTCAGGATATTTTTCCCA+AGG + chr7.4:9197007-9197026 MS.gene007520:CDS 35.0%
! TGGAATGAAAGACGGAATTT+TGG - chr7.4:9196721-9196740 None:intergenic 35.0%
!! TTTGATAATGATGGTGTTGC+TGG + chr7.4:9196837-9196856 MS.gene007520:CDS 35.0%
ACTTCATCAGAAAAGCTAGG+AGG + chr7.4:9199032-9199051 MS.gene007520:CDS 40.0%
AGCGTTTCTGCACAATATCT+AGG - chr7.4:9196954-9196973 None:intergenic 40.0%
AGGACTCATCAAGGATAAGT+TGG + chr7.4:9197567-9197586 MS.gene007520:CDS 40.0%
AGTTTCTTGTCGCTGACAAA+AGG - chr7.4:9197113-9197132 None:intergenic 40.0%
ATCATTCACGGCTGAAACAA+AGG - chr7.4:9198227-9198246 None:intergenic 40.0%
ATCCAGCTCTACAATTGAAC+CGG - chr7.4:9199120-9199139 None:intergenic 40.0%
ATCTTCATGTTGGAATTCGG+AGG + chr7.4:9197177-9197196 MS.gene007520:CDS 40.0%
ATGTGAAAAGAGTGAATCCG+TGG + chr7.4:9198300-9198319 MS.gene007520:CDS 40.0%
CAAACAATACAAGTCGACAC+TGG + chr7.4:9198770-9198789 MS.gene007520:CDS 40.0%
CAATCTGATGCAAACAATGG+TGG + chr7.4:9196915-9196934 MS.gene007520:CDS 40.0%
CACATAGAATCAGACCATGA+CGG - chr7.4:9197629-9197648 None:intergenic 40.0%
CACTATATTGCTATGCCTTG+TGG + chr7.4:9197818-9197837 MS.gene007520:intron 40.0%
CATTTCAGGCACGTTTATAG+GGG + chr7.4:9197050-9197069 MS.gene007520:CDS 40.0%
CGAAGAAACGGTAAAGGTAA+TGG + chr7.4:9197329-9197348 MS.gene007520:CDS 40.0%
GAAATGTTCTCACTGGTGTT+CGG - chr7.4:9198668-9198687 None:intergenic 40.0%
GACTCCATATTCATCTCAGT+TGG - chr7.4:9196537-9196556 None:intergenic 40.0%
GAGACTTCATCAGAAAAGCT+AGG + chr7.4:9199029-9199048 MS.gene007520:intron 40.0%
GATGACTGTCCCTAATAACT+TGG + chr7.4:9198631-9198650 MS.gene007520:CDS 40.0%
GCATTTCAGGCACGTTTATA+GGG + chr7.4:9197049-9197068 MS.gene007520:CDS 40.0%
GCGATCTTCATGTTGGAATT+CGG + chr7.4:9197174-9197193 MS.gene007520:CDS 40.0%
GGAAAAATATCCTGAAGAGG+AGG - chr7.4:9197005-9197024 None:intergenic 40.0%
GTAAAATATGTGAAGGCCCT+CGG - chr7.4:9198015-9198034 None:intergenic 40.0%
GTTGTCAACAAATGCCTCTT+CGG - chr7.4:9197080-9197099 None:intergenic 40.0%
GTTTGCATCAGATTGTGTCA+AGG - chr7.4:9196910-9196929 None:intergenic 40.0%
TAAAATATGTGAAGGCCCTC+GGG - chr7.4:9198014-9198033 None:intergenic 40.0%
TAATATGCAGCCGTTTGATG+TGG + chr7.4:9197430-9197449 MS.gene007520:CDS 40.0%
TCTTCATGTTGGAATTCGGA+GGG + chr7.4:9197178-9197197 MS.gene007520:CDS 40.0%
TGATGAGTCCTCAGTTTCAA+TGG - chr7.4:9197558-9197577 None:intergenic 40.0%
TGCACAATCTTCAGAGAAAC+CGG + chr7.4:9198712-9198731 MS.gene007520:CDS 40.0%
TTAGCTGAATAGTCCAAACG+AGG - chr7.4:9196981-9197000 None:intergenic 40.0%
TTGAAACTGAGGACTCATCA+AGG + chr7.4:9197558-9197577 MS.gene007520:CDS 40.0%
TTGGAACAGCACTTCAGATT+AGG + chr7.4:9197504-9197523 MS.gene007520:CDS 40.0%
TTGGAGTCGATCCTTTGTTA+GGG + chr7.4:9197219-9197238 MS.gene007520:CDS 40.0%
! ACATGTTTTCTACTTCCCTC+AGG + chr7.4:9196650-9196669 MS.gene007520:CDS 40.0%
! CATGTTTTCTACTTCCCTCA+GGG + chr7.4:9196651-9196670 MS.gene007520:CDS 40.0%
! TTTGGCCAAAAAGCACAAGT+TGG - chr7.4:9198754-9198773 None:intergenic 40.0%
! TTTTCGCCATGAACATCCTT+GGG - chr7.4:9197026-9197045 None:intergenic 40.0%
!! AAGAGGCATTTGTTGACAAC+TGG + chr7.4:9197080-9197099 MS.gene007520:CDS 40.0%
!! AAGTGATGGAATGATGGGTA+GGG + chr7.4:9197361-9197380 MS.gene007520:CDS 40.0%
!! CAGTGTCGACTTGTATTGTT+TGG - chr7.4:9198772-9198791 None:intergenic 40.0%
!! GAGTAAGCACTGAAATTCAC+CGG - chr7.4:9196699-9196718 None:intergenic 40.0%
!! TAAGTGATGGAATGATGGGT+AGG + chr7.4:9197360-9197379 MS.gene007520:CDS 40.0%
!! TGGAAATCTGGTAGTGGAAT+TGG + chr7.4:9197242-9197261 MS.gene007520:CDS 40.0%
!!! AGAAACGCTTTTTCCTCGTT+TGG + chr7.4:9196965-9196984 MS.gene007520:CDS 40.0%
AAAATATGTGAAGGCCCTCG+GGG - chr7.4:9198013-9198032 None:intergenic 45.0%
AACGATGGAAATCCTCCGTA+AGG - chr7.4:9197284-9197303 None:intergenic 45.0%
AGGATGTTCATGGCGAAAAG+TGG + chr7.4:9197027-9197046 MS.gene007520:CDS 45.0%
ATGGCGAAAAGTGGCATTTC+AGG + chr7.4:9197036-9197055 MS.gene007520:CDS 45.0%
ATTGGGAAATCTGGGAGTTG+AGG - chr7.4:9198401-9198420 None:intergenic 45.0%
ATTTGTCCTGCACCACCTTA+CGG + chr7.4:9197266-9197285 MS.gene007520:CDS 45.0%
CAAGATGGCCATTGAAACTG+AGG + chr7.4:9197547-9197566 MS.gene007520:CDS 45.0%
CAATGCGAAGAAACATTCGC+AGG + chr7.4:9198899-9198918 MS.gene007520:CDS 45.0%
CATACATTAGGTTTCCCCGA+GGG + chr7.4:9197996-9198015 MS.gene007520:intron 45.0%
CCAGATTTCCACCCTAACAA+AGG - chr7.4:9197233-9197252 None:intergenic 45.0%
CCTTTGTTAGGGTGGAAATC+TGG + chr7.4:9197230-9197249 MS.gene007520:CDS 45.0%
CGAGCATGAAATGTTCTCAC+TGG - chr7.4:9198675-9198694 None:intergenic 45.0%
CGGAACTATTGCTTCTGTTC+AGG + chr7.4:9197595-9197614 MS.gene007520:CDS 45.0%
CGTCATGGTCTGATTCTATG+TGG + chr7.4:9197627-9197646 MS.gene007520:CDS 45.0%
CTAGGCTGAAAGAGACCTAA+TGG - chr7.4:9198572-9198591 None:intergenic 45.0%
CTCCCAGATTTCCCAATTGA+TGG + chr7.4:9198404-9198423 MS.gene007520:CDS 45.0%
CTCTCATCAGATGTACGTGT+CGG - chr7.4:9198857-9198876 None:intergenic 45.0%
CTGCGAATGTTTCTTCGCAT+TGG - chr7.4:9198901-9198920 None:intergenic 45.0%
GAAAACGGCGATCTTCATGT+TGG + chr7.4:9197167-9197186 MS.gene007520:CDS 45.0%
GAACAGCACTTCAGATTAGG+TGG + chr7.4:9197507-9197526 MS.gene007520:CDS 45.0%
GGCATTTCAGGCACGTTTAT+AGG + chr7.4:9197048-9197067 MS.gene007520:CDS 45.0%
GGCCATCAATTGGGAAATCT+GGG - chr7.4:9198409-9198428 None:intergenic 45.0%
GTGGTTTATTATCCTCGGTC+AGG + chr7.4:9197449-9197468 MS.gene007520:CDS 45.0%
GTTGGAGTCGATCCTTTGTT+AGG + chr7.4:9197218-9197237 MS.gene007520:CDS 45.0%
TCATACATTAGGTTTCCCCG+AGG + chr7.4:9197995-9198014 MS.gene007520:intron 45.0%
TCCTGGAATGAGGTTCAAGA+TGG + chr7.4:9197532-9197551 MS.gene007520:CDS 45.0%
TCTGATGCAAACAATGGTGG+TGG + chr7.4:9196918-9196937 MS.gene007520:CDS 45.0%
TGGCCATCAATTGGGAAATC+TGG - chr7.4:9198410-9198429 None:intergenic 45.0%
TTAGGGTGGAAATCTGGTAG+TGG + chr7.4:9197236-9197255 MS.gene007520:CDS 45.0%
TTCTATGTGGAGACTTCTCG+AGG + chr7.4:9197640-9197659 MS.gene007520:CDS 45.0%
TTCTTCCTTGGCGGTATGAA+AGG - chr7.4:9198368-9198387 None:intergenic 45.0%
TTGGAATTCGGAGGGCTAAA+AGG + chr7.4:9197186-9197205 MS.gene007520:CDS 45.0%
! CGGAGGATTTCCATCGTTTT+CGG + chr7.4:9197286-9197305 MS.gene007520:CDS 45.0%
! CTTTTCGCCATGAACATCCT+TGG - chr7.4:9197027-9197046 None:intergenic 45.0%
! GGAGGATTTCCATCGTTTTC+GGG + chr7.4:9197287-9197306 MS.gene007520:CDS 45.0%
! GGCATTTGTTGACAACTGGA+TGG + chr7.4:9197084-9197103 MS.gene007520:CDS 45.0%
!! AGTGATGGAATGATGGGTAG+GGG + chr7.4:9197362-9197381 MS.gene007520:CDS 45.0%
!! ATTTCCATCGTTTTCGGGAG+AGG + chr7.4:9197292-9197311 MS.gene007520:CDS 45.0%
!! CCAAAAAGCACAAGTTGGTG+CGG - chr7.4:9198749-9198768 None:intergenic 45.0%
AAAGGCGAAAGAGGGATCGA+AGG - chr7.4:9198350-9198369 None:intergenic 50.0%
AAGACCGAAATGACCATGCC+TGG - chr7.4:9198525-9198544 None:intergenic 50.0%
AGCGACAAGAAACTTGCGTC+TGG + chr7.4:9197119-9197138 MS.gene007520:CDS 50.0%
CAGCTCTACAATTGAACCGG+TGG - chr7.4:9199117-9199136 None:intergenic 50.0%
CATCTGATGAGAGGTTCGAG+TGG + chr7.4:9198864-9198883 MS.gene007520:CDS 50.0%
CGACAAGAAACTTGCGTCTG+GGG + chr7.4:9197121-9197140 MS.gene007520:CDS 50.0%
CGACACGTACATCTGATGAG+AGG + chr7.4:9198855-9198874 MS.gene007520:CDS 50.0%
CTTCAGATTAGGTGGTGTCC+TGG + chr7.4:9197515-9197534 MS.gene007520:CDS 50.0%
GAGTCGATCCTTTGTTAGGG+TGG + chr7.4:9197222-9197241 MS.gene007520:CDS 50.0%
GATGGAAATCCTCCGTAAGG+TGG - chr7.4:9197281-9197300 None:intergenic 50.0%
GCCATCTTGAACCTCATTCC+AGG - chr7.4:9197536-9197555 None:intergenic 50.0%
GCGACAAGAAACTTGCGTCT+GGG + chr7.4:9197120-9197139 MS.gene007520:CDS 50.0%
GCGGTATGAAAGGCGAAAGA+GGG - chr7.4:9198358-9198377 None:intergenic 50.0%
TCTTCCTCTCCCGAAAACGA+TGG - chr7.4:9197299-9197318 None:intergenic 50.0%
TTACCCGAAACTAGTCCTGC+CGG + chr7.4:9198488-9198507 MS.gene007520:CDS 50.0%
TTAGGTGGTGTCCTGGAATG+AGG + chr7.4:9197522-9197541 MS.gene007520:CDS 50.0%
TTCAGCTAACCCTCCTCTTC+AGG + chr7.4:9196992-9197011 MS.gene007520:CDS 50.0%
TTGCACCATTCCTCCTGCAA+CGG - chr7.4:9196613-9196632 None:intergenic 50.0%
TTTATAGGGGAACGCCGAAG+AGG + chr7.4:9197063-9197082 MS.gene007520:CDS 50.0%
TTTCGCCTTTCATACCGCCA+AGG + chr7.4:9198360-9198379 MS.gene007520:CDS 50.0%
! CCGCACCAACTTGTGCTTTT+TGG + chr7.4:9198746-9198765 MS.gene007520:CDS 50.0%
!! CACCGGTTCAATTGTAGAGC+TGG + chr7.4:9199115-9199134 MS.gene007520:CDS 50.0%
!! GTGATGGAATGATGGGTAGG+GGG + chr7.4:9197363-9197382 MS.gene007520:CDS 50.0%
AATCCTCCGTAAGGTGGTGC+AGG - chr7.4:9197275-9197294 None:intergenic 55.0%
AGAGTGTGGACCCTCAGCTA+TGG + chr7.4:9196583-9196602 MS.gene007520:CDS 55.0%
CAACGGCATGCCATAGCTGA+GGG - chr7.4:9196596-9196615 None:intergenic 55.0%
GGAGCCAGGCATGGTCATTT+CGG + chr7.4:9198518-9198537 MS.gene007520:CDS 55.0%
GGCGGTATGAAAGGCGAAAG+AGG - chr7.4:9198359-9198378 None:intergenic 55.0%
TGGTGCGGCTTTGCATGATC+CGG - chr7.4:9198734-9198753 None:intergenic 55.0%
TTCAGGCTGCTGATCCGTCA+TGG + chr7.4:9197612-9197631 MS.gene007520:CDS 55.0%
TTCCACTAGCGGCGCGAAAA+TGG + chr7.4:9199072-9199091 MS.gene007520:CDS 55.0%
! CATCAGATGTACGTGTCGGC+AGG - chr7.4:9198853-9198872 None:intergenic 55.0%
!! AACTCAGGAGTACCTGACCG+AGG - chr7.4:9197464-9197483 None:intergenic 55.0%
!! ATGCCGGCAGGACTAGTTTC+GGG - chr7.4:9198494-9198513 None:intergenic 55.0%
!! CACCGGTTCACATGCATGCT+CGG - chr7.4:9196682-9196701 None:intergenic 55.0%
!! CATGCCGGCAGGACTAGTTT+CGG - chr7.4:9198495-9198514 None:intergenic 55.0%
!! TGGAATGATGGGTAGGGGGA+AGG + chr7.4:9197367-9197386 MS.gene007520:CDS 55.0%
AACTAGTCCTGCCGGCATGC+AGG + chr7.4:9198496-9198515 MS.gene007520:CDS 60.0%
ACTAGTCCTGCCGGCATGCA+GGG + chr7.4:9198497-9198516 MS.gene007520:CDS 60.0%
CACTAGCGGCGCGAAAATGG+AGG + chr7.4:9199075-9199094 MS.gene007520:CDS 60.0%
CAGCTATGGCATGCCGTTGC+AGG + chr7.4:9196597-9196616 MS.gene007520:CDS 60.0%
CGCCGAGCATGCATGTGAAC+CGG + chr7.4:9196677-9196696 MS.gene007520:CDS 60.0%
CTATGGCATGCCGTTGCAGG+AGG + chr7.4:9196600-9196619 MS.gene007520:CDS 60.0%
GCAACGGCATGCCATAGCTG+AGG - chr7.4:9196597-9196616 None:intergenic 60.0%
GCATGCCGTTGCAGGAGGAA+TGG + chr7.4:9196605-9196624 MS.gene007520:CDS 60.0%
TGTCCTGCACCACCTTACGG+AGG + chr7.4:9197269-9197288 MS.gene007520:CDS 60.0%
! CTCCATTTTCGCGCCGCTAG+TGG - chr7.4:9199077-9199096 None:intergenic 60.0%
ATGCCTGGCTCCCTGCATGC+CGG - chr7.4:9198510-9198529 None:intergenic 65.0%
CATGCTCGGCGTGACCCTGA+GGG - chr7.4:9196668-9196687 None:intergenic 65.0%
GCATGCTCGGCGTGACCCTG+AGG - chr7.4:9196669-9196688 None:intergenic 70.0%
GGCATGCAGGGAGCCAGGCA+TGG + chr7.4:9198509-9198528 MS.gene007520:CDS 70.0%
CTGCCGGCATGCAGGGAGCC+AGG + chr7.4:9198504-9198523 MS.gene007520:CDS 75.0%
CTGGCTCCCTGCATGCCGGC+AGG - chr7.4:9198506-9198525 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 9196507 9199141 9196507 ID=MS.gene007520
chr7.4 mRNA 9196507 9199141 9196507 ID=MS.gene007520.t1;Parent=MS.gene007520
chr7.4 exon 9196507 9197661 9196507 ID=MS.gene007520.t1.exon1;Parent=MS.gene007520.t1
chr7.4 CDS 9196507 9197661 9196507 ID=cds.MS.gene007520.t1;Parent=MS.gene007520.t1
chr7.4 exon 9198269 9198947 9198269 ID=MS.gene007520.t1.exon2;Parent=MS.gene007520.t1
chr7.4 CDS 9198269 9198947 9198269 ID=cds.MS.gene007520.t1;Parent=MS.gene007520.t1
chr7.4 exon 9199032 9199141 9199032 ID=MS.gene007520.t1.exon3;Parent=MS.gene007520.t1
chr7.4 CDS 9199032 9199141 9199032 ID=cds.MS.gene007520.t1;Parent=MS.gene007520.t1
Gene Sequence

>MS.gene007520

ATGAAAGAAAAAAGAAAAGGTTGCAATCCAACTGAGATGAATATGGAGTCAAACAAGAAATTGAAAAATGTGAACAAGAGTGTGGACCCTCAGCTATGGCATGCCGTTGCAGGAGGAATGGTGCAAATGCCAGAAGTTAACTCACATGTTTTCTACTTCCCTCAGGGTCACGCCGAGCATGCATGTGAACCGGTGAATTTCAGTGCTTACTCCAAAATTCCGTCTTTCATTCCATGTAGAGTTGAAGCAATAAGATATATGGCTAATCATGAGACCGATGAAGTGTATGCAAAACTCAGGCTTGTTCCTATGAATATTAACCAAGTTAGTTTTGATAATGATGGTGTTGCTGGCATCAACGTGTCTGAAATTAAGGATAAACATCAATCTTTTGCGAAAACCTTGACACAATCTGATGCAAACAATGGTGGTGGCTTTTCTTGTCCTAGATATTGTGCAGAAACGCTTTTTCCTCGTTTGGACTATTCAGCTAACCCTCCTCTTCAGGATATTTTTCCCAAGGATGTTCATGGCGAAAAGTGGCATTTCAGGCACGTTTATAGGGGAACGCCGAAGAGGCATTTGTTGACAACTGGATGGAGTCCTTTTGTCAGCGACAAGAAACTTGCGTCTGGGGATTCGATTGTGTTTTTGAGATCTGAAAACGGCGATCTTCATGTTGGAATTCGGAGGGCTAAAAGGCGAAATAATGTTGGAGTCGATCCTTTGTTAGGGTGGAAATCTGGTAGTGGAATTGGAATTTGTCCTGCACCACCTTACGGAGGATTTCCATCGTTTTCGGGAGAGGAAGATAATAAACTTCGAAGAAACGGTAAAGGTAATGGATTGCTAATAAGTGATGGAATGATGGGTAGGGGGAAGGTGAAAGCTCTAGAAGTTATTGAAGCAATAAAACTCGGTACTAATATGCAGCCGTTTGATGTGGTTTATTATCCTCGGTCAGGTACTCCTGAGTTTCTTGTGAAAACTTCTTTAATTGGAACAGCACTTCAGATTAGGTGGTGTCCTGGAATGAGGTTCAAGATGGCCATTGAAACTGAGGACTCATCAAGGATAAGTTGGTTTATCGGAACTATTGCTTCTGTTCAGGCTGCTGATCCGTCATGGTCTGATTCTATGTGGAGACTTCTCGAGGTATATACATGTTTATTATACACTCCCTCTAGAATTAATTTACTTGAGAATTTGTTTTATTGATGAATGTTTTGAGAATAATAACGGTAAATACAGTAAAGTTAGTTAGATTTTACTTATATTTTGGTCAACGTTGTCATATAGCAGCTATAGTGGCACTATATTGCTATGCCTTGTGGAATTTATACAAATCACTATTGTTCTACCATAGAATGTGTATTCTTGCAACATTTTCTTTTTCCAAAGCATAATCCTTTTAAGTTTTCATCAGTACCATAAATTGGAAGCTTCCTATTATTAGAATTTGCTAAAAATCCTATACCTACATCACTATCATACATTAGGTTTCCCCGAGGGCCTTCACATATTTTACTTTGAATACATTCAAAATTTGCAAGTTAATGTATATATTTGTGTATGGTAATATAGATACAATGGAACATTGTTGGTACCTAATGAAATTGTAAGAAGAGAATTAGATTAGAATTTCATACTAATGAAATTCACAGTGTCCTTTTTATTGCATATATTACTATGTCATTTTGAATTACATTAATTTTGACCTTTGTTTCAGCCGTGAATGATTTTCTGATGTCATACTTTTTAGGTTACATGGGATGAACCTGAGATACTTAAAAATGTGAAAAGAGTGAATCCGTGGCAAGTTGAAATAGTATCGAACATGCCTTCGATCCCTCTTTCGCCTTTCATACCGCCAAGGAAGAAATTGAGATTACCTCAACTCCCAGATTTCCCAATTGATGGCCAATTTCCAATGCCAACTTTTCCAAACAACCTTCTAAATCCTAATATTCCAATTTTTTATTTACCCGAAACTAGTCCTGCCGGCATGCAGGGAGCCAGGCATGGTCATTTCGGTCTTACCTTACCAGATTTTCATAATCTTCCATTAGGTCTCTTTCAGCCTAGTTTCCAACAACCGTTTAATAATAATATTGCGACGATGCCGATGACTGTCCCTAATAACTTGGCATTGCAAAAGCCGAACACCAGTGAGAACATTTCATGCTCGCATTCGATATCAACTTCTGCACAATCTTCAGAGAAACCGGATCATGCAAAGCCGCACCAACTTGTGCTTTTTGGCCAAACAATACAAGTCGACACTGGAAATGAGAATTCCGAGAAGAAAATGACTAATCATTTGTCTGATTTTCATCTACAAAGCCTGCCGACACGTACATCTGATGAGAGGTTCGAGTGGAATGCTAAGAACCAATGCGAAGAAACATTCGCAGGAGAAACATTAGAGATGAAACATTCAGGTAAGAATGAATAAAATTCAGTATCTTTCTACTAGAAATTGATTTATGATATATGATAATAACTTATTTGTCTCTCTTTCAGAGACTTCATCAGAAAAGCTAGGAGGTTGACAATTTTAATGGATTCCACTAGCGGCGCGAAAATGGAGGATCAGCAAGAAAAAAACCACCGGTTCAATTGTAGAGCTGGATGA
Protein sequence

>MS.gene007520.t1

MKEKRKGCNPTEMNMESNKKLKNVNKSVDPQLWHAVAGGMVQMPEVNSHVFYFPQGHAEHACEPVNFSAYSKIPSFIPCRVEAIRYMANHETDEVYAKLRLVPMNINQVSFDNDGVAGINVSEIKDKHQSFAKTLTQSDANNGGGFSCPRYCAETLFPRLDYSANPPLQDIFPKDVHGEKWHFRHVYRGTPKRHLLTTGWSPFVSDKKLASGDSIVFLRSENGDLHVGIRRAKRRNNVGVDPLLGWKSGSGIGICPAPPYGGFPSFSGEEDNKLRRNGKGNGLLISDGMMGRGKVKALEVIEAIKLGTNMQPFDVVYYPRSGTPEFLVKTSLIGTALQIRWCPGMRFKMAIETEDSSRISWFIGTIASVQAADPSWSDSMWRLLEVTWDEPEILKNVKRVNPWQVEIVSNMPSIPLSPFIPPRKKLRLPQLPDFPIDGQFPMPTFPNNLLNPNIPIFYLPETSPAGMQGARHGHFGLTLPDFHNLPLGLFQPSFQQPFNNNIATMPMTVPNNLALQKPNTSENISCSHSISTSAQSSEKPDHAKPHQLVLFGQTIQVDTGNENSEKKMTNHLSDFHLQSLPTRTSDERFEWNAKNQCEETFAGETLEMKHSDFIRKARRLTILMDSTSGAKMEDQQEKNHRFNCRAG