Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007720.t1 | XP_013455940.1 | 92 | 238 | 17 | 1 | 12 | 249 | 89 | 324 | 7.20E-121 | 443.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007720.t1 | F4JSU0 | 62.7 | 51 | 19 | 0 | 12 | 62 | 89 | 139 | 4.0e-11 | 70.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007720.t1 | A0A072UKP5 | 92.0 | 238 | 17 | 1 | 12 | 249 | 89 | 324 | 5.2e-121 | 443.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene007720.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007720.t1 | MTR_4g057635 | 93.697 | 238 | 13 | 1 | 12 | 249 | 89 | 324 | 1.79e-136 | 387 |
| MS.gene007720.t1 | MTR_2g095520 | 40.157 | 254 | 112 | 9 | 12 | 247 | 89 | 320 | 9.71e-23 | 95.1 |
| MS.gene007720.t1 | MTR_5g014990 | 73.171 | 41 | 11 | 0 | 12 | 52 | 89 | 129 | 1.63e-13 | 69.3 |
| MS.gene007720.t1 | MTR_1g017140 | 75.610 | 41 | 9 | 1 | 3 | 42 | 79 | 119 | 1.03e-12 | 67.0 |
| MS.gene007720.t1 | MTR_6g009430 | 67.500 | 40 | 13 | 0 | 12 | 51 | 88 | 127 | 1.91e-12 | 66.2 |
| MS.gene007720.t1 | MTR_3g461490 | 70.000 | 40 | 12 | 0 | 12 | 51 | 88 | 127 | 2.24e-12 | 65.9 |
| MS.gene007720.t1 | MTR_3g103570 | 72.973 | 37 | 10 | 0 | 6 | 42 | 83 | 119 | 4.16e-12 | 65.5 |
| MS.gene007720.t1 | MTR_4g082290 | 52.308 | 65 | 30 | 1 | 2 | 65 | 77 | 141 | 1.20e-11 | 64.3 |
| MS.gene007720.t1 | MTR_3g045430 | 56.140 | 57 | 17 | 1 | 12 | 68 | 89 | 137 | 1.30e-11 | 63.5 |
| MS.gene007720.t1 | MTR_4g082230 | 52.308 | 65 | 30 | 1 | 2 | 65 | 77 | 141 | 1.40e-11 | 63.9 |
| MS.gene007720.t1 | MTR_6g090405 | 83.871 | 31 | 5 | 0 | 12 | 42 | 89 | 119 | 2.86e-11 | 62.4 |
| MS.gene007720.t1 | MTR_1g076150 | 75.676 | 37 | 9 | 0 | 4 | 40 | 28 | 64 | 3.73e-11 | 61.2 |
| MS.gene007720.t1 | MTR_3g074520 | 60.870 | 46 | 18 | 0 | 8 | 53 | 84 | 129 | 4.35e-11 | 62.4 |
| MS.gene007720.t1 | MTR_8g031360 | 49.231 | 65 | 27 | 1 | 1 | 65 | 79 | 137 | 6.49e-11 | 61.6 |
| MS.gene007720.t1 | MTR_1g017000 | 77.419 | 31 | 7 | 0 | 12 | 42 | 89 | 119 | 8.99e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007720.t1 | AT4G37780 | 60.784 | 51 | 20 | 0 | 6 | 56 | 83 | 133 | 2.51e-14 | 71.6 |
| MS.gene007720.t1 | AT5G57620 | 76.744 | 43 | 9 | 1 | 3 | 44 | 79 | 121 | 1.74e-13 | 69.3 |
| MS.gene007720.t1 | AT3G49690 | 70.455 | 44 | 13 | 0 | 6 | 49 | 83 | 126 | 2.49e-13 | 68.6 |
| MS.gene007720.t1 | AT5G65790 | 63.830 | 47 | 17 | 0 | 6 | 52 | 83 | 129 | 2.91e-13 | 68.9 |
| MS.gene007720.t1 | AT5G56110 | 68.182 | 44 | 14 | 0 | 8 | 51 | 84 | 127 | 4.26e-13 | 68.2 |
| MS.gene007720.t1 | AT1G22640 | 51.724 | 58 | 28 | 0 | 7 | 64 | 11 | 68 | 6.58e-13 | 65.9 |
| MS.gene007720.t1 | AT3G61250 | 44.737 | 76 | 36 | 1 | 1 | 70 | 77 | 152 | 1.16e-12 | 66.6 |
| MS.gene007720.t1 | AT5G23000 | 32.215 | 149 | 72 | 5 | 6 | 141 | 83 | 215 | 3.72e-12 | 65.5 |
| MS.gene007720.t1 | AT5G15310 | 50.000 | 56 | 28 | 0 | 4 | 59 | 1 | 56 | 5.64e-12 | 64.3 |
| MS.gene007720.t1 | AT4G12350 | 40.909 | 88 | 38 | 2 | 1 | 88 | 77 | 150 | 1.41e-11 | 63.5 |
| MS.gene007720.t1 | AT5G10280 | 50.000 | 74 | 32 | 2 | 8 | 80 | 84 | 153 | 2.07e-11 | 63.2 |
| MS.gene007720.t1 | AT5G65230 | 58.696 | 46 | 19 | 0 | 8 | 53 | 84 | 129 | 2.42e-11 | 63.2 |
Find 38 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAAACATGTTATCTTTAAT+AGG | 0.206533 | 4.4:+45720902 | None:intergenic |
| TAACCATGGACTCTTGATTT+TGG | 0.209482 | 4.4:+45720957 | None:intergenic |
| AAGAACAACTTTATGATAAA+TGG | 0.255168 | 4.4:-45720746 | MS.gene007720:CDS |
| TTAAATCCATTACTAGATTA+TGG | 0.290043 | 4.4:-45720629 | MS.gene007720:CDS |
| TAAACATGTTATCTTTAATA+GGG | 0.351159 | 4.4:+45720903 | None:intergenic |
| AAAGATAACATGTTTATGTT+TGG | 0.366890 | 4.4:-45720896 | MS.gene007720:CDS |
| ACAATGATATCAAGAACTAT+TGG | 0.403975 | 4.4:-45721177 | MS.gene007720:CDS |
| GTTATTTGAAGGAAGTGAAA+TGG | 0.408479 | 4.4:+45721009 | None:intergenic |
| GTTGGATATAGTTATAATAA+TGG | 0.408482 | 4.4:-45720665 | MS.gene007720:CDS |
| AGTGGTGGATTTGAATTTGA+TGG | 0.411324 | 4.4:-45720779 | MS.gene007720:CDS |
| ATAGGGTTGAAACTTGTGTT+TGG | 0.411863 | 4.4:+45720983 | None:intergenic |
| TCTAAGAATTGATGAGAAGA+AGG | 0.426803 | 4.4:+45721073 | None:intergenic |
| GAAGGAGAAGGTGGAAATGA+AGG | 0.428759 | 4.4:+45721091 | None:intergenic |
| AATAGGGTAATTATATTGCA+TGG | 0.439492 | 4.4:+45720919 | None:intergenic |
| GGTAACAATGGAAGTATTGT+TGG | 0.458840 | 4.4:-45720725 | MS.gene007720:CDS |
| TGAAGATCTATGAAGGAAGT+TGG | 0.463076 | 4.4:+45721034 | None:intergenic |
| TTTCTTTGATGATGTAATGT+TGG | 0.475351 | 4.4:+45721115 | None:intergenic |
| TATCATCATCACATGAATAG+TGG | 0.484541 | 4.4:-45720797 | MS.gene007720:CDS |
| GTGTTTGGATAGTTATTTGA+AGG | 0.484722 | 4.4:+45720998 | None:intergenic |
| ATTCATAAGAATGAAGAGAT+TGG | 0.485771 | 4.4:-45720545 | MS.gene007720:CDS |
| TGATGAGAAGAAGGAGAAGG+TGG | 0.495664 | 4.4:+45721082 | None:intergenic |
| ATAGGGTAATTATATTGCAT+GGG | 0.509971 | 4.4:+45720920 | None:intergenic |
| ATATCATTGTCTGTCCTACC+TGG | 0.529658 | 4.4:+45721190 | None:intergenic |
| ATGTTTATGTTTGGAAGTGA+AGG | 0.530266 | 4.4:-45720887 | MS.gene007720:CDS |
| ATTATAGCAGCTCAATTGCC+AGG | 0.535265 | 4.4:-45721208 | MS.gene007720:CDS |
| ATTAGTAGCAGCAGCAACAA+AGG | 0.539539 | 4.4:-45720584 | MS.gene007720:CDS |
| AATGGTTTGAAGATCTATGA+AGG | 0.546814 | 4.4:+45721027 | None:intergenic |
| TTACCAAAATCAAGAGTCCA+TGG | 0.554120 | 4.4:-45720960 | MS.gene007720:CDS |
| GAAGGAAGTAGTTCATCTGA+TGG | 0.557638 | 4.4:-45720869 | MS.gene007720:CDS |
| TTAGTAGCAGCAGCAACAAA+GGG | 0.558041 | 4.4:-45720583 | MS.gene007720:CDS |
| TCAAGACCATAATCTAGTAA+TGG | 0.566224 | 4.4:+45720623 | None:intergenic |
| TAGCAGCTCAATTGCCAGGT+AGG | 0.572910 | 4.4:-45721204 | MS.gene007720:CDS |
| TTTATGATAAATGGTAACAA+TGG | 0.578179 | 4.4:-45720737 | MS.gene007720:CDS |
| AATTGATGAGAAGAAGGAGA+AGG | 0.583152 | 4.4:+45721079 | None:intergenic |
| GGGTAATTATATTGCATGGG+AGG | 0.586340 | 4.4:+45720923 | None:intergenic |
| CATCATCACATGAATAGTGG+TGG | 0.600970 | 4.4:-45720794 | MS.gene007720:CDS |
| AGGATTAACAACACTAACCA+TGG | 0.605158 | 4.4:+45720943 | None:intergenic |
| GGAAGTTGCACTCTTAGTCA+TGG | 0.605348 | 4.4:-45720848 | MS.gene007720:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAACATGTTATCTTTAAT+AGG | + | chr4.4:45720874-45720893 | None:intergenic | 15.0% |
| !! | TAAACATGTTATCTTTAATA+GGG | + | chr4.4:45720873-45720892 | None:intergenic | 15.0% |
| !!! | CATTTTTGATATTGATTTAT+AGG | - | chr4.4:45720536-45720555 | MS.gene007720:CDS | 15.0% |
| !! | AAAGATAACATGTTTATGTT+TGG | - | chr4.4:45720877-45720896 | MS.gene007720:CDS | 20.0% |
| !! | AAGAACAACTTTATGATAAA+TGG | - | chr4.4:45721027-45721046 | MS.gene007720:CDS | 20.0% |
| !! | GTTGGATATAGTTATAATAA+TGG | - | chr4.4:45721108-45721127 | MS.gene007720:CDS | 20.0% |
| !! | TTAAATCCATTACTAGATTA+TGG | - | chr4.4:45721144-45721163 | MS.gene007720:CDS | 20.0% |
| !! | TTTATGATAAATGGTAACAA+TGG | - | chr4.4:45721036-45721055 | MS.gene007720:CDS | 20.0% |
| !!! | TTTTGATATTGATTTATAGG+TGG | - | chr4.4:45720539-45720558 | MS.gene007720:CDS | 20.0% |
| ! | AAAGTTGAAGAAAAAACTCA+TGG | - | chr4.4:45720624-45720643 | MS.gene007720:CDS | 25.0% |
| ! | AATAGGGTAATTATATTGCA+TGG | + | chr4.4:45720857-45720876 | None:intergenic | 25.0% |
| ! | ACAATGATATCAAGAACTAT+TGG | - | chr4.4:45720596-45720615 | MS.gene007720:CDS | 25.0% |
| ! | ATAGGGTAATTATATTGCAT+GGG | + | chr4.4:45720856-45720875 | None:intergenic | 25.0% |
| ! | ATTCATAAGAATGAAGAGAT+TGG | - | chr4.4:45721228-45721247 | MS.gene007720:CDS | 25.0% |
| ! | CAATATGAAGAAAAATCAAG+TGG | - | chr4.4:45721084-45721103 | MS.gene007720:CDS | 25.0% |
| !! | CACTTGATTTTTCTTCATAT+TGG | + | chr4.4:45721086-45721105 | None:intergenic | 25.0% |
| !!! | ACTCTTGATTTTGGTAAATA+GGG | + | chr4.4:45720810-45720829 | None:intergenic | 25.0% |
| !!! | TTTCTTTGATGATGTAATGT+TGG | + | chr4.4:45720661-45720680 | None:intergenic | 25.0% |
| AATGGTTTGAAGATCTATGA+AGG | + | chr4.4:45720749-45720768 | None:intergenic | 30.0% | |
| ATGTTTATGTTTGGAAGTGA+AGG | - | chr4.4:45720886-45720905 | MS.gene007720:CDS | 30.0% | |
| GTGTTTGGATAGTTATTTGA+AGG | + | chr4.4:45720778-45720797 | None:intergenic | 30.0% | |
| GTTATTTGAAGGAAGTGAAA+TGG | + | chr4.4:45720767-45720786 | None:intergenic | 30.0% | |
| TATCATCATCACATGAATAG+TGG | - | chr4.4:45720976-45720995 | MS.gene007720:CDS | 30.0% | |
| TCAAGACCATAATCTAGTAA+TGG | + | chr4.4:45721153-45721172 | None:intergenic | 30.0% | |
| TCTAAGAATTGATGAGAAGA+AGG | + | chr4.4:45720703-45720722 | None:intergenic | 30.0% | |
| !! | GACTCTTGATTTTGGTAAAT+AGG | + | chr4.4:45720811-45720830 | None:intergenic | 30.0% |
| AATTGATGAGAAGAAGGAGA+AGG | + | chr4.4:45720697-45720716 | None:intergenic | 35.0% | |
| AGGATTAACAACACTAACCA+TGG | + | chr4.4:45720833-45720852 | None:intergenic | 35.0% | |
| ATAGGGTTGAAACTTGTGTT+TGG | + | chr4.4:45720793-45720812 | None:intergenic | 35.0% | |
| GAAGAAAAATCAAGTGGAGT+TGG | - | chr4.4:45721090-45721109 | MS.gene007720:CDS | 35.0% | |
| TGAAGATCTATGAAGGAAGT+TGG | + | chr4.4:45720742-45720761 | None:intergenic | 35.0% | |
| TTACCAAAATCAAGAGTCCA+TGG | - | chr4.4:45720813-45720832 | MS.gene007720:CDS | 35.0% | |
| ! | TAACCATGGACTCTTGATTT+TGG | + | chr4.4:45720819-45720838 | None:intergenic | 35.0% |
| !! | AGTGGTGGATTTGAATTTGA+TGG | - | chr4.4:45720994-45721013 | MS.gene007720:CDS | 35.0% |
| !! | GGTAACAATGGAAGTATTGT+TGG | - | chr4.4:45721048-45721067 | MS.gene007720:CDS | 35.0% |
| ATATCATTGTCTGTCCTACC+TGG | + | chr4.4:45720586-45720605 | None:intergenic | 40.0% | |
| ATTAGTAGCAGCAGCAACAA+AGG | - | chr4.4:45721189-45721208 | MS.gene007720:CDS | 40.0% | |
| ATTATAGCAGCTCAATTGCC+AGG | - | chr4.4:45720565-45720584 | MS.gene007720:CDS | 40.0% | |
| CATCATCACATGAATAGTGG+TGG | - | chr4.4:45720979-45720998 | MS.gene007720:CDS | 40.0% | |
| GAAGGAAGTAGTTCATCTGA+TGG | - | chr4.4:45720904-45720923 | MS.gene007720:CDS | 40.0% | |
| GGGTAATTATATTGCATGGG+AGG | + | chr4.4:45720853-45720872 | None:intergenic | 40.0% | |
| TTAGTAGCAGCAGCAACAAA+GGG | - | chr4.4:45721190-45721209 | MS.gene007720:CDS | 40.0% | |
| GAAGGAGAAGGTGGAAATGA+AGG | + | chr4.4:45720685-45720704 | None:intergenic | 45.0% | |
| GGAAGTTGCACTCTTAGTCA+TGG | - | chr4.4:45720925-45720944 | MS.gene007720:CDS | 45.0% | |
| TGATGAGAAGAAGGAGAAGG+TGG | + | chr4.4:45720694-45720713 | None:intergenic | 45.0% | |
| TAGCAGCTCAATTGCCAGGT+AGG | - | chr4.4:45720569-45720588 | MS.gene007720:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 45720523 | 45721272 | 45720523 | ID=MS.gene007720 |
| chr4.4 | mRNA | 45720523 | 45721272 | 45720523 | ID=MS.gene007720.t1;Parent=MS.gene007720 |
| chr4.4 | exon | 45720523 | 45721272 | 45720523 | ID=MS.gene007720.t1.exon1;Parent=MS.gene007720.t1 |
| chr4.4 | CDS | 45720523 | 45721272 | 45720523 | ID=cds.MS.gene007720.t1;Parent=MS.gene007720.t1 |
| Gene Sequence |
| Protein sequence |
