Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007743.t1 | AES76827.2 | 45.5 | 325 | 110 | 11 | 1 | 282 | 9 | 309 | 9.60E-61 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007743.t1 | G7KPD8 | 45.5 | 325 | 110 | 11 | 1 | 282 | 9 | 309 | 6.9e-61 | 243.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene007743.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007743.t1 | MTR_6g087720 | 50.482 | 311 | 101 | 12 | 1 | 282 | 23 | 309 | 3.51e-88 | 265 |
| MS.gene007743.t1 | MTR_3g088985 | 48.993 | 149 | 52 | 6 | 1 | 135 | 1 | 139 | 4.84e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 62 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCCATTGATTGTTTCAATT+TGG | 0.155668 | 4.4:-45997093 | None:intergenic |
| TCCATTAGATTCTTCTGATT+TGG | 0.203292 | 4.4:+45997435 | MS.gene007743:CDS |
| CATGATCAATTCATTTATAA+AGG | 0.248719 | 4.4:+45997685 | MS.gene007743:CDS |
| CCATGCTTGGTCAAGAATTC+TGG | 0.256325 | 4.4:+45997652 | MS.gene007743:CDS |
| GAATCTAATGGAACTATAAA+AGG | 0.283886 | 4.4:-45997424 | None:intergenic |
| CCATTAGATTCTTCTGATTT+GGG | 0.303530 | 4.4:+45997436 | MS.gene007743:CDS |
| TCAAATCACTAACTCGAATA+AGG | 0.309419 | 4.4:-45997051 | None:intergenic |
| GAAACTTGGTGCAAGAAATA+TGG | 0.324099 | 4.4:+45997184 | MS.gene007743:CDS |
| CTGTAGCGTTTATGTTCCTC+TGG | 0.347837 | 4.4:-45997220 | None:intergenic |
| ATGCTACTAATTTGAATACT+TGG | 0.354531 | 4.4:+45997503 | MS.gene007743:CDS |
| AGACGATGAGAGTTGTCATT+TGG | 0.365480 | 4.4:-45997337 | None:intergenic |
| ATTGTTTCAATTTGGATTGT+TGG | 0.388306 | 4.4:-45997085 | None:intergenic |
| TCTATGAACACTTCGAAACT+TGG | 0.399054 | 4.4:+45997170 | MS.gene007743:CDS |
| CCCAAATCAGAAGAATCTAA+TGG | 0.410187 | 4.4:-45997436 | None:intergenic |
| TGTTAGATCGACAAGAGTTA+AGG | 0.431807 | 4.4:+45997720 | MS.gene007743:CDS |
| AACCTCCTTGAAATACTCCT+TGG | 0.433670 | 4.4:-45997565 | None:intergenic |
| GACGATGAGAGTTGTCATTT+GGG | 0.440001 | 4.4:-45997336 | None:intergenic |
| ATCCAAATTGAAACAATCAA+TGG | 0.446001 | 4.4:+45997091 | MS.gene007743:CDS |
| ACTCGAATAAGGCGTTGAAT+TGG | 0.448084 | 4.4:-45997040 | None:intergenic |
| TTTCATTAAAGTCATTGATC+GGG | 0.458302 | 4.4:+45997612 | MS.gene007743:CDS |
| ACTCCTTGGGAAATTCAGTA+TGG | 0.473075 | 4.4:-45997551 | None:intergenic |
| TCCAAATTGAAACAATCAAT+GGG | 0.473791 | 4.4:+45997092 | MS.gene007743:CDS |
| ATCAATTCATTTATAAAGGA+TGG | 0.474045 | 4.4:+45997689 | MS.gene007743:CDS |
| ACCTCCTTGAAATACTCCTT+GGG | 0.474430 | 4.4:-45997564 | None:intergenic |
| ATTTAATTCTTATGAACTGA+AGG | 0.485251 | 4.4:+45997117 | MS.gene007743:CDS |
| AAAGTCATTGATCGGGAGTC+CGG | 0.485891 | 4.4:+45997619 | MS.gene007743:CDS |
| ATTTCCCAAGGAGTATTTCA+AGG | 0.485980 | 4.4:+45997560 | MS.gene007743:CDS |
| CATCATTCCGTTCTTTGCCA+TGG | 0.495906 | 4.4:-45997764 | None:intergenic |
| CCAGAATTCTTGACCAAGCA+TGG | 0.497468 | 4.4:-45997652 | None:intergenic |
| GTTTCATTAAAGTCATTGAT+CGG | 0.502036 | 4.4:+45997611 | MS.gene007743:CDS |
| CTACAGGTTCAATCTGTTCA+AGG | 0.503789 | 4.4:+45997237 | MS.gene007743:CDS |
| TGAGCGAAGCCTCACGCCAT+TGG | 0.504131 | 4.4:-45996966 | None:intergenic |
| TGCATGATAATCTCGATGAC+TGG | 0.513746 | 4.4:+45997011 | MS.gene007743:CDS |
| TGGGAAATTCAGTATGGTCA+TGG | 0.514882 | 4.4:-45997545 | None:intergenic |
| ATGGAACTATAAAAGGTTGT+CGG | 0.519300 | 4.4:-45997417 | None:intergenic |
| TGTAGCGTTTATGTTCCTCT+GGG | 0.521857 | 4.4:-45997219 | None:intergenic |
| CATCGAGAAAATGCAGAGAA+AGG | 0.527404 | 4.4:-45997294 | None:intergenic |
| GCAATGTATCTGCACAAGAT+TGG | 0.541189 | 4.4:+45997391 | MS.gene007743:CDS |
| TTCGAAGTGTTCATAGAAGT+CGG | 0.541979 | 4.4:-45997164 | None:intergenic |
| CAGAGGAACATAAACGCTAC+AGG | 0.546873 | 4.4:+45997221 | MS.gene007743:CDS |
| ATTTGGATTGTTGGTATCAG+CGG | 0.551199 | 4.4:-45997076 | None:intergenic |
| GTCATCGAGATTATCATGCA+TGG | 0.567674 | 4.4:-45997008 | None:intergenic |
| ATCTTCACCATGGCAAAGAA+CGG | 0.576109 | 4.4:+45997757 | MS.gene007743:CDS |
| TAGATCGACAAGAGTTAAGG+AGG | 0.588263 | 4.4:+45997723 | MS.gene007743:CDS |
| AGCATGGATGAGCGCCACGA+TGG | 0.589465 | 4.4:+45997367 | MS.gene007743:CDS |
| TTTGAATACTTGGAAAACGA+AGG | 0.589603 | 4.4:+45997513 | MS.gene007743:CDS |
| AATGACAACTCTCATCGTCT+AGG | 0.594641 | 4.4:+45997340 | MS.gene007743:CDS |
| TCAATGACTTTAATGAAACA+AGG | 0.613809 | 4.4:-45997607 | None:intergenic |
| TCCAGAATGAGTCTCTCCAA+TGG | 0.619199 | 4.4:+45996950 | None:intergenic |
| CAAGCATGGAAAATGTGTAC+CGG | 0.629661 | 4.4:-45997638 | None:intergenic |
| GCATGATAATCTCGATGACT+GGG | 0.643893 | 4.4:+45997012 | MS.gene007743:CDS |
| TGAGTCTCTCCAATGGCGTG+AGG | 0.649032 | 4.4:+45996957 | MS.gene007743:CDS |
| AAAGAACGGAATGATGATGT+TGG | 0.656487 | 4.4:+45997771 | MS.gene007743:CDS |
| TCATCGTCTAGGCTGCAGCA+TGG | 0.662339 | 4.4:+45997351 | MS.gene007743:CDS |
| TGACCATACTGAATTTCCCA+AGG | 0.667492 | 4.4:+45997548 | MS.gene007743:CDS |
| TCCCAAGGAGTATTTCAAGG+AGG | 0.692766 | 4.4:+45997563 | MS.gene007743:CDS |
| AGATCGACAAGAGTTAAGGA+GGG | 0.692933 | 4.4:+45997724 | MS.gene007743:CDS |
| GCAGATACATTGCGCCATCG+TGG | 0.701988 | 4.4:-45997381 | None:intergenic |
| TGATAGTCTGATCTTCACCA+TGG | 0.702037 | 4.4:+45997747 | MS.gene007743:CDS |
| GTAGCGTTTATGTTCCTCTG+GGG | 0.719905 | 4.4:-45997218 | None:intergenic |
| ACTGAAGGAAACAATCAATG+CGG | 0.772990 | 4.4:+45997132 | MS.gene007743:CDS |
| TGGCAAAACATACTCCCCAG+AGG | 0.774295 | 4.4:+45997204 | MS.gene007743:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATTCATTTATAAAGGA+TGG | + | chr4.4:45997689-45997708 | MS.gene007743:CDS | 20.0% |
| !! | ATTTAATTCTTATGAACTGA+AGG | + | chr4.4:45997117-45997136 | MS.gene007743:CDS | 20.0% |
| !! | CATGATCAATTCATTTATAA+AGG | + | chr4.4:45997685-45997704 | MS.gene007743:CDS | 20.0% |
| ! | ATCCAAATTGAAACAATCAA+TGG | + | chr4.4:45997091-45997110 | MS.gene007743:CDS | 25.0% |
| ! | ATGCTACTAATTTGAATACT+TGG | + | chr4.4:45997503-45997522 | MS.gene007743:CDS | 25.0% |
| ! | ATTGTTTCAATTTGGATTGT+TGG | - | chr4.4:45997088-45997107 | None:intergenic | 25.0% |
| ! | GAATCTAATGGAACTATAAA+AGG | - | chr4.4:45997427-45997446 | None:intergenic | 25.0% |
| ! | GTTTCATTAAAGTCATTGAT+CGG | + | chr4.4:45997611-45997630 | MS.gene007743:CDS | 25.0% |
| ! | TCAATGACTTTAATGAAACA+AGG | - | chr4.4:45997610-45997629 | None:intergenic | 25.0% |
| ! | TCCAAATTGAAACAATCAAT+GGG | + | chr4.4:45997092-45997111 | MS.gene007743:CDS | 25.0% |
| ! | TTTCATTAAAGTCATTGATC+GGG | + | chr4.4:45997612-45997631 | MS.gene007743:CDS | 25.0% |
| ATGGAACTATAAAAGGTTGT+CGG | - | chr4.4:45997420-45997439 | None:intergenic | 30.0% | |
| CCATTAGATTCTTCTGATTT+GGG | + | chr4.4:45997436-45997455 | MS.gene007743:CDS | 30.0% | |
| TCAAATCACTAACTCGAATA+AGG | - | chr4.4:45997054-45997073 | None:intergenic | 30.0% | |
| TCCATTAGATTCTTCTGATT+TGG | + | chr4.4:45997435-45997454 | MS.gene007743:CDS | 30.0% | |
| TTTGAATACTTGGAAAACGA+AGG | + | chr4.4:45997513-45997532 | MS.gene007743:CDS | 30.0% | |
| ! | ACTTTTGTTAGGAGGATTAA+TGG | - | chr4.4:45997272-45997291 | None:intergenic | 30.0% |
| ! | AGAGAAAGGTAACTTTTGTT+AGG | - | chr4.4:45997283-45997302 | None:intergenic | 30.0% |
| ! | CTTTTGTTAGGAGGATTAAT+GGG | - | chr4.4:45997271-45997290 | None:intergenic | 30.0% |
| ! | TCCCATTGATTGTTTCAATT+TGG | - | chr4.4:45997096-45997115 | None:intergenic | 30.0% |
| !!! | GAAACAAGGATTGTTTTTGT+GGG | - | chr4.4:45997596-45997615 | None:intergenic | 30.0% |
| !!! | TGAAACAAGGATTGTTTTTG+TGG | - | chr4.4:45997597-45997616 | None:intergenic | 30.0% |
| ACTGAAGGAAACAATCAATG+CGG | + | chr4.4:45997132-45997151 | MS.gene007743:CDS | 35.0% | |
| ATTTGGATTGTTGGTATCAG+CGG | - | chr4.4:45997079-45997098 | None:intergenic | 35.0% | |
| CCCAAATCAGAAGAATCTAA+TGG | - | chr4.4:45997439-45997458 | None:intergenic | 35.0% | |
| TCTATGAACACTTCGAAACT+TGG | + | chr4.4:45997170-45997189 | MS.gene007743:CDS | 35.0% | |
| TGTTAGATCGACAAGAGTTA+AGG | + | chr4.4:45997720-45997739 | MS.gene007743:CDS | 35.0% | |
| ! | AAAGAACGGAATGATGATGT+TGG | + | chr4.4:45997771-45997790 | MS.gene007743:CDS | 35.0% |
| ! | GAAAGGTAACTTTTGTTAGG+AGG | - | chr4.4:45997280-45997299 | None:intergenic | 35.0% |
| ! | TTCGAAGTGTTCATAGAAGT+CGG | - | chr4.4:45997167-45997186 | None:intergenic | 35.0% |
| !! | ATTTCCCAAGGAGTATTTCA+AGG | + | chr4.4:45997560-45997579 | MS.gene007743:CDS | 35.0% |
| !! | GAAACTTGGTGCAAGAAATA+TGG | + | chr4.4:45997184-45997203 | MS.gene007743:CDS | 35.0% |
| AACCTCCTTGAAATACTCCT+TGG | - | chr4.4:45997568-45997587 | None:intergenic | 40.0% | |
| AATGACAACTCTCATCGTCT+AGG | + | chr4.4:45997340-45997359 | MS.gene007743:CDS | 40.0% | |
| ACCTCCTTGAAATACTCCTT+GGG | - | chr4.4:45997567-45997586 | None:intergenic | 40.0% | |
| ACTCCTTGGGAAATTCAGTA+TGG | - | chr4.4:45997554-45997573 | None:intergenic | 40.0% | |
| AGACGATGAGAGTTGTCATT+TGG | - | chr4.4:45997340-45997359 | None:intergenic | 40.0% | |
| AGATCGACAAGAGTTAAGGA+GGG | + | chr4.4:45997724-45997743 | MS.gene007743:CDS | 40.0% | |
| ATCTTCACCATGGCAAAGAA+CGG | + | chr4.4:45997757-45997776 | MS.gene007743:CDS | 40.0% | |
| CAAGCATGGAAAATGTGTAC+CGG | - | chr4.4:45997641-45997660 | None:intergenic | 40.0% | |
| CATCGAGAAAATGCAGAGAA+AGG | - | chr4.4:45997297-45997316 | None:intergenic | 40.0% | |
| CTACAGGTTCAATCTGTTCA+AGG | + | chr4.4:45997237-45997256 | MS.gene007743:CDS | 40.0% | |
| GCAATGTATCTGCACAAGAT+TGG | + | chr4.4:45997391-45997410 | MS.gene007743:CDS | 40.0% | |
| GCATGATAATCTCGATGACT+GGG | + | chr4.4:45997012-45997031 | MS.gene007743:CDS | 40.0% | |
| GTCATCGAGATTATCATGCA+TGG | - | chr4.4:45997011-45997030 | None:intergenic | 40.0% | |
| TAGATCGACAAGAGTTAAGG+AGG | + | chr4.4:45997723-45997742 | MS.gene007743:CDS | 40.0% | |
| TGACCATACTGAATTTCCCA+AGG | + | chr4.4:45997548-45997567 | MS.gene007743:CDS | 40.0% | |
| TGATAGTCTGATCTTCACCA+TGG | + | chr4.4:45997747-45997766 | MS.gene007743:CDS | 40.0% | |
| TGCATGATAATCTCGATGAC+TGG | + | chr4.4:45997011-45997030 | MS.gene007743:CDS | 40.0% | |
| TGGGAAATTCAGTATGGTCA+TGG | - | chr4.4:45997548-45997567 | None:intergenic | 40.0% | |
| TGTAGCGTTTATGTTCCTCT+GGG | - | chr4.4:45997222-45997241 | None:intergenic | 40.0% | |
| ! | GACGATGAGAGTTGTCATTT+GGG | - | chr4.4:45997339-45997358 | None:intergenic | 40.0% |
| !! | ACTCGAATAAGGCGTTGAAT+TGG | - | chr4.4:45997043-45997062 | None:intergenic | 40.0% |
| AAAGTCATTGATCGGGAGTC+CGG | + | chr4.4:45997619-45997638 | MS.gene007743:CDS | 45.0% | |
| CAGAGGAACATAAACGCTAC+AGG | + | chr4.4:45997221-45997240 | MS.gene007743:CDS | 45.0% | |
| CATCATTCCGTTCTTTGCCA+TGG | - | chr4.4:45997767-45997786 | None:intergenic | 45.0% | |
| CCAGAATTCTTGACCAAGCA+TGG | - | chr4.4:45997655-45997674 | None:intergenic | 45.0% | |
| CCATGCTTGGTCAAGAATTC+TGG | + | chr4.4:45997652-45997671 | MS.gene007743:CDS | 45.0% | |
| CTGTAGCGTTTATGTTCCTC+TGG | - | chr4.4:45997223-45997242 | None:intergenic | 45.0% | |
| GTAGCGTTTATGTTCCTCTG+GGG | - | chr4.4:45997221-45997240 | None:intergenic | 45.0% | |
| ! | CGGTACACATTTTCCATGCT+TGG | + | chr4.4:45997639-45997658 | MS.gene007743:CDS | 45.0% |
| !! | TCCCAAGGAGTATTTCAAGG+AGG | + | chr4.4:45997563-45997582 | MS.gene007743:CDS | 45.0% |
| TGGCAAAACATACTCCCCAG+AGG | + | chr4.4:45997204-45997223 | MS.gene007743:CDS | 50.0% | |
| GCAGATACATTGCGCCATCG+TGG | - | chr4.4:45997384-45997403 | None:intergenic | 55.0% | |
| TCATCGTCTAGGCTGCAGCA+TGG | + | chr4.4:45997351-45997370 | MS.gene007743:CDS | 55.0% | |
| TGAGTCTCTCCAATGGCGTG+AGG | + | chr4.4:45996957-45996976 | MS.gene007743:CDS | 55.0% | |
| AGCATGGATGAGCGCCACGA+TGG | + | chr4.4:45997367-45997386 | MS.gene007743:CDS | 60.0% | |
| TGAGCGAAGCCTCACGCCAT+TGG | - | chr4.4:45996969-45996988 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 45996956 | 45997816 | 45996956 | ID=MS.gene007743 |
| chr4.4 | mRNA | 45996956 | 45997816 | 45996956 | ID=MS.gene007743.t1;Parent=MS.gene007743 |
| chr4.4 | exon | 45996956 | 45997816 | 45996956 | ID=MS.gene007743.t1.exon1;Parent=MS.gene007743.t1 |
| chr4.4 | CDS | 45996956 | 45997816 | 45996956 | ID=cds.MS.gene007743.t1;Parent=MS.gene007743.t1 |
| Gene Sequence |
| Protein sequence |
