Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007889.t1 | XP_003628534.1 | 98.9 | 375 | 4 | 0 | 1 | 375 | 1 | 375 | 1.10E-210 | 742.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007889.t1 | Q8RWJ7 | 69.9 | 296 | 74 | 4 | 1 | 281 | 1 | 296 | 1.4e-113 | 411.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007889.t1 | G7LDQ2 | 98.9 | 375 | 4 | 0 | 1 | 375 | 1 | 375 | 8.1e-211 | 742.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene007889.t1 | TF | HB-other |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007889.t1 | MTR_8g061040 | 98.933 | 375 | 4 | 0 | 1 | 375 | 1 | 375 | 0.0 | 778 |
| MS.gene007889.t1 | MTR_3g110260 | 40.076 | 262 | 125 | 4 | 1 | 260 | 1 | 232 | 7.02e-65 | 206 |
| MS.gene007889.t1 | MTR_8g014075 | 38.372 | 258 | 148 | 3 | 1 | 258 | 1 | 247 | 1.02e-49 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007889.t1 | AT3G18380 | 69.932 | 296 | 74 | 4 | 1 | 281 | 1 | 296 | 1.04e-151 | 432 |
| MS.gene007889.t1 | AT3G18380 | 69.697 | 297 | 74 | 5 | 1 | 281 | 1 | 297 | 7.77e-150 | 427 |
| MS.gene007889.t1 | AT3G18380 | 69.728 | 294 | 76 | 5 | 1 | 281 | 1 | 294 | 3.13e-149 | 425 |
| MS.gene007889.t1 | AT1G15215 | 43.725 | 247 | 135 | 2 | 14 | 259 | 14 | 257 | 1.86e-63 | 203 |
| MS.gene007889.t1 | AT1G15215 | 42.979 | 235 | 128 | 3 | 14 | 246 | 14 | 244 | 1.21e-56 | 186 |
| MS.gene007889.t1 | AT1G15215 | 42.979 | 235 | 128 | 3 | 14 | 246 | 14 | 244 | 1.53e-56 | 186 |
| MS.gene007889.t1 | AT1G15215 | 35.256 | 156 | 92 | 4 | 14 | 167 | 14 | 162 | 9.04e-19 | 83.2 |
Find 90 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTGAGTCATTATCCTTCC+TGG | 0.211207 | 8.3:+47322256 | None:intergenic |
| GAAGGCGGGGCCTCCGTTAC+TGG | 0.217756 | 8.3:+47325249 | None:intergenic |
| TGTAATCTGTAATCAGTTTC+AGG | 0.218700 | 8.3:+47317346 | None:intergenic |
| GAAACATTTGTTTGCAAAAC+AGG | 0.257239 | 8.3:+47317175 | None:intergenic |
| AAAATAAGCGATATGCTATA+AGG | 0.259455 | 8.3:-47322342 | MS.gene007889:CDS |
| GTTCCTGCTCCTTCAGCTTC+AGG | 0.261491 | 8.3:-47322220 | MS.gene007889:intron |
| AAGCACTTGCAGACAAGTTT+AGG | 0.288349 | 8.3:-47322602 | MS.gene007889:intron |
| GGCGTGATATTTAACTTTGC+AGG | 0.330713 | 8.3:+47322301 | None:intergenic |
| CTTTCTATCATACAGACATT+TGG | 0.352765 | 8.3:-47319463 | MS.gene007889:CDS |
| ATGGAATTTGAAGCTAAATC+TGG | 0.358674 | 8.3:-47319608 | MS.gene007889:CDS |
| CTCGCACCATGAACATTTGC+TGG | 0.369463 | 8.3:+47317262 | None:intergenic |
| TTTCATTGCAGGTATGGAAT+TGG | 0.369862 | 8.3:-47322369 | MS.gene007889:intron |
| GTTGAGTCATTATCCTTCCT+GGG | 0.392666 | 8.3:+47322257 | None:intergenic |
| CCAGTGAATGCGGCGGTGCT+TGG | 0.419350 | 8.3:+47317073 | None:intergenic |
| ATCTGTATAGGTAACAGAAA+TGG | 0.427116 | 8.3:-47322676 | MS.gene007889:intron |
| GGATCCACATTCATACTTTG+TGG | 0.428698 | 8.3:+47317145 | None:intergenic |
| AAATAAGCGATATGCTATAA+GGG | 0.442347 | 8.3:-47322341 | MS.gene007889:CDS |
| CCAAGCACCGCCGCATTCAC+TGG | 0.450582 | 8.3:-47317073 | MS.gene007889:CDS |
| GGCTGAGTGAAACGGAAGGC+GGG | 0.454503 | 8.3:+47325235 | None:intergenic |
| GGTTACTAATCCTTCTGAAA+TGG | 0.459416 | 8.3:-47317239 | MS.gene007889:CDS |
| TGTAATCTTGCCTTTACGAT+CGG | 0.459596 | 8.3:+47322470 | None:intergenic |
| GAAGCTGAAGGAGCAGGAAC+AGG | 0.470238 | 8.3:+47322223 | None:intergenic |
| GTAATCTTGCCTTTACGATC+GGG | 0.473723 | 8.3:+47322471 | None:intergenic |
| GTCTTTGAGCATCAAGGACA+TGG | 0.475882 | 8.3:+47318651 | None:intergenic |
| ATATTCAAGTTACTTCCCTT+CGG | 0.477909 | 8.3:+47317003 | None:intergenic |
| GACATGGGCATCAAAGTAAA+GGG | 0.481099 | 8.3:+47318667 | None:intergenic |
| CAAATGTCTGTATGATAGAA+AGG | 0.481293 | 8.3:+47319464 | None:intergenic |
| GTCAAATCAGTCCGAGGCAT+GGG | 0.481986 | 8.3:+47322280 | None:intergenic |
| GATCATCCAGCAAATGTTCA+TGG | 0.483799 | 8.3:-47317268 | MS.gene007889:CDS |
| CGGGGCCTCCGTTACTGGGT+GGG | 0.497571 | 8.3:+47325254 | None:intergenic |
| GATTACAGTACAGATGAAAC+AGG | 0.498566 | 8.3:-47322454 | MS.gene007889:intron |
| GGACATGGGCATCAAAGTAA+AGG | 0.499318 | 8.3:+47318666 | None:intergenic |
| GCCAGGAAATCGTGCCGCTT+AGG | 0.503677 | 8.3:-47317384 | MS.gene007889:intron |
| AAGGCGGGGCCTCCGTTACT+GGG | 0.508017 | 8.3:+47325250 | None:intergenic |
| ACAAAAGCTGATGTTCAAGC+TGG | 0.509935 | 8.3:-47317109 | MS.gene007889:CDS |
| CTACCTGAAGCTGAAGGAGC+AGG | 0.516322 | 8.3:+47322217 | None:intergenic |
| GAAGCTAAATCTGGAAGAGA+TGG | 0.517358 | 8.3:-47319599 | MS.gene007889:CDS |
| CCTCGGACTGATTTGACCCC+AGG | 0.520581 | 8.3:-47322274 | MS.gene007889:CDS |
| CACAATCTTCTGCTGATCCG+TGG | 0.523298 | 8.3:+47317294 | None:intergenic |
| CAAGTTACTTCCCTTCGGCC+AGG | 0.523928 | 8.3:+47317008 | None:intergenic |
| ACATTTGGAGAGCAGTGATC+CGG | 0.538548 | 8.3:-47319448 | MS.gene007889:intron |
| AGACGGAGGCATGATGTAAG+AGG | 0.539794 | 8.3:-47318632 | MS.gene007889:CDS |
| TAAATATCACGCCCATGCCT+CGG | 0.541650 | 8.3:-47322291 | MS.gene007889:CDS |
| AAACAAAGTATGAGATCACC+AGG | 0.544394 | 8.3:+47318831 | None:intergenic |
| TGCCGCTTAGGAAGATTTGT+CGG | 0.552355 | 8.3:-47317372 | MS.gene007889:CDS |
| GCTGAGTGAAACGGAAGGCG+GGG | 0.554948 | 8.3:+47325236 | None:intergenic |
| ATACCTCGGGCTGAGTGAAA+CGG | 0.555409 | 8.3:+47325227 | None:intergenic |
| GCGGGGCCTCCGTTACTGGG+TGG | 0.555777 | 8.3:+47325253 | None:intergenic |
| AGTGAATCACCCGATCGTAA+AGG | 0.556434 | 8.3:-47322480 | MS.gene007889:CDS |
| GGCTCCTGAAAATTCAGTTA+TGG | 0.559539 | 8.3:-47319627 | MS.gene007889:CDS |
| ACAATCTTCTGCTGATCCGT+GGG | 0.561174 | 8.3:+47317295 | None:intergenic |
| GCTTGAAGAACATCTCGTGC+TGG | 0.567998 | 8.3:+47322621 | None:intergenic |
| CAGCAAATGTTCATGGTGCG+AGG | 0.569607 | 8.3:-47317261 | MS.gene007889:CDS |
| GGTCAAATCAGTCCGAGGCA+TGG | 0.569910 | 8.3:+47322279 | None:intergenic |
| GGGCTGAGTGAAACGGAAGG+CGG | 0.572841 | 8.3:+47325234 | None:intergenic |
| GAATGTGTCGCAGTGCTCCC+TGG | 0.583022 | 8.3:-47318849 | MS.gene007889:CDS |
| ATGGGTCGCCCACCCAGTAA+CGG | 0.584649 | 8.3:-47325262 | MS.gene007889:CDS |
| ATTAATCCACTCATCTTCCT+CGG | 0.585484 | 8.3:+47318917 | None:intergenic |
| CTACTCAAAACCTGGCCGAA+GGG | 0.589785 | 8.3:-47317018 | MS.gene007889:CDS |
| TCTACTCAAAACCTGGCCGA+AGG | 0.590738 | 8.3:-47317019 | MS.gene007889:CDS |
| GCCTCCGTCTTTGAGCATCA+AGG | 0.590882 | 8.3:+47318645 | None:intergenic |
| TTGAGTCATTATCCTTCCTG+GGG | 0.591497 | 8.3:+47322258 | None:intergenic |
| GCAGGAACAGGAGAAGCTGT+TGG | 0.594628 | 8.3:+47322235 | None:intergenic |
| GACTTCAGGAACACTGCTAG+TGG | 0.595794 | 8.3:+47317042 | None:intergenic |
| TGAAGTCTCTACTCAAAACC+TGG | 0.601287 | 8.3:-47317026 | MS.gene007889:CDS |
| GGCAATGATTCCAGTGAATG+CGG | 0.603755 | 8.3:+47317063 | None:intergenic |
| GGATCAGCAGAAGATTGTGT+TGG | 0.605323 | 8.3:-47317290 | MS.gene007889:CDS |
| CACAGTTCAAACAACAGCAA+AGG | 0.607569 | 8.3:-47319651 | MS.gene007889:intron |
| GATTCACATGGCAGAGAGCG+CGG | 0.607948 | 8.3:+47318876 | None:intergenic |
| ATGTCCTTGATGCTCAAAGA+CGG | 0.608783 | 8.3:-47318649 | MS.gene007889:CDS |
| AGCAAATGTTCATGGTGCGA+GGG | 0.609406 | 8.3:-47317260 | MS.gene007889:CDS |
| AACATTCTACCTGAAGCTGA+AGG | 0.613833 | 8.3:+47322211 | None:intergenic |
| TCCACATTCATACTTTGTGG+TGG | 0.615610 | 8.3:+47317148 | None:intergenic |
| TTGGATCCGAGGAAGATGAG+TGG | 0.615863 | 8.3:-47318923 | MS.gene007889:CDS |
| ACCACCACAAAGTATGAATG+TGG | 0.618600 | 8.3:-47317149 | MS.gene007889:CDS |
| CTTCCGTTTCACTCAGCCCG+AGG | 0.628138 | 8.3:-47325230 | MS.gene007889:intron |
| AGTTCAAACAACAGCAAAGG+CGG | 0.631634 | 8.3:-47319648 | MS.gene007889:CDS |
| TGTGAATGATGCAGCACCCA+CGG | 0.641428 | 8.3:-47317311 | MS.gene007889:CDS |
| CTATGATCACGATCAATCTG+AGG | 0.641893 | 8.3:-47318585 | MS.gene007889:intron |
| AATCTGGAAGAGATGGAGCA+TGG | 0.658527 | 8.3:-47319592 | MS.gene007889:intron |
| CTCCGACAAATCTTCCTAAG+CGG | 0.665093 | 8.3:+47317370 | None:intergenic |
| CCTGGGGTCAAATCAGTCCG+AGG | 0.675720 | 8.3:+47322274 | None:intergenic |
| TCCTTGATGCTCAAAGACGG+AGG | 0.675814 | 8.3:-47318646 | MS.gene007889:CDS |
| GGACTGATTTGACCCCAGGA+AGG | 0.679224 | 8.3:-47322270 | MS.gene007889:CDS |
| GGTCGCCCACCCAGTAACGG+AGG | 0.681262 | 8.3:-47325259 | MS.gene007889:CDS |
| ACACATTCTGATGATTCACA+TGG | 0.682055 | 8.3:+47318864 | None:intergenic |
| TCTTTGAGCATCAAGGACAT+GGG | 0.687814 | 8.3:+47318652 | None:intergenic |
| CTCGGGCTGAGTGAAACGGA+AGG | 0.689498 | 8.3:+47325231 | None:intergenic |
| AATGATTCCAGTGAATGCGG+CGG | 0.736741 | 8.3:+47317066 | None:intergenic |
| AACAAAGTATGAGATCACCA+GGG | 0.773298 | 8.3:+47318832 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 47317012 | 47325284 | 47317012 | ID=MS.gene007889 |
| chr8.3 | mRNA | 47317012 | 47325284 | 47317012 | ID=MS.gene007889.t1;Parent=MS.gene007889 |
| chr8.3 | exon | 47325231 | 47325284 | 47325231 | ID=MS.gene007889.t1.exon1;Parent=MS.gene007889.t1 |
| chr8.3 | CDS | 47325231 | 47325284 | 47325231 | ID=cds.MS.gene007889.t1;Parent=MS.gene007889.t1 |
| chr8.3 | exon | 47322603 | 47322688 | 47322603 | ID=MS.gene007889.t1.exon2;Parent=MS.gene007889.t1 |
| chr8.3 | CDS | 47322603 | 47322688 | 47322603 | ID=cds.MS.gene007889.t1;Parent=MS.gene007889.t1 |
| chr8.3 | exon | 47322455 | 47322500 | 47322455 | ID=MS.gene007889.t1.exon3;Parent=MS.gene007889.t1 |
| chr8.3 | CDS | 47322455 | 47322500 | 47322455 | ID=cds.MS.gene007889.t1;Parent=MS.gene007889.t1 |
| chr8.3 | exon | 47322221 | 47322380 | 47322221 | ID=MS.gene007889.t1.exon4;Parent=MS.gene007889.t1 |
| chr8.3 | CDS | 47322221 | 47322380 | 47322221 | ID=cds.MS.gene007889.t1;Parent=MS.gene007889.t1 |
| chr8.3 | exon | 47319593 | 47319668 | 47319593 | ID=MS.gene007889.t1.exon5;Parent=MS.gene007889.t1 |
| chr8.3 | CDS | 47319593 | 47319668 | 47319593 | ID=cds.MS.gene007889.t1;Parent=MS.gene007889.t1 |
| chr8.3 | exon | 47319449 | 47319500 | 47319449 | ID=MS.gene007889.t1.exon6;Parent=MS.gene007889.t1 |
| chr8.3 | CDS | 47319449 | 47319500 | 47319449 | ID=cds.MS.gene007889.t1;Parent=MS.gene007889.t1 |
| chr8.3 | exon | 47318827 | 47318970 | 47318827 | ID=MS.gene007889.t1.exon7;Parent=MS.gene007889.t1 |
| chr8.3 | CDS | 47318827 | 47318970 | 47318827 | ID=cds.MS.gene007889.t1;Parent=MS.gene007889.t1 |
| chr8.3 | exon | 47318586 | 47318705 | 47318586 | ID=MS.gene007889.t1.exon8;Parent=MS.gene007889.t1 |
| chr8.3 | CDS | 47318586 | 47318705 | 47318586 | ID=cds.MS.gene007889.t1;Parent=MS.gene007889.t1 |
| chr8.3 | exon | 47317012 | 47317401 | 47317012 | ID=MS.gene007889.t1.exon9;Parent=MS.gene007889.t1 |
| chr8.3 | CDS | 47317012 | 47317401 | 47317012 | ID=cds.MS.gene007889.t1;Parent=MS.gene007889.t1 |
| Gene Sequence |
| Protein sequence |
