AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007947

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007947.t1 MTR_3g072710 97.638 508 8 2 1 504 1 508 0.0 1019
MS.gene007947.t1 MTR_5g058860 63.022 503 148 14 12 499 21 500 0.0 579
MS.gene007947.t1 MTR_8g093070 38.718 390 201 12 116 496 136 496 6.43e-67 224
MS.gene007947.t1 MTR_7g027190 33.607 488 273 15 30 497 218 674 3.04e-65 223
MS.gene007947.t1 MTR_3g065980 31.195 452 255 13 76 502 126 546 6.17e-53 187
MS.gene007947.t1 MTR_7g074650 37.203 379 201 16 124 495 443 791 1.08e-51 188
MS.gene007947.t1 MTR_1g069725 31.100 418 253 12 89 500 79 467 6.02e-49 175
MS.gene007947.t1 MTR_4g076020 36.828 372 193 13 133 498 96 431 1.12e-48 174
MS.gene007947.t1 MTR_3g089055 32.099 405 237 12 104 495 190 569 3.32e-48 175
MS.gene007947.t1 MTR_3g089055 32.099 405 237 12 104 495 185 564 4.42e-48 175
MS.gene007947.t1 MTR_1g029420 32.821 390 228 11 118 495 224 591 5.60e-48 175
MS.gene007947.t1 MTR_4g104020 31.683 404 252 9 103 497 133 521 2.10e-47 172
MS.gene007947.t1 MTR_8g442410 31.674 442 260 13 81 499 106 528 4.20e-45 166
MS.gene007947.t1 MTR_4g077760 32.609 368 229 8 131 495 203 554 7.60e-45 166
MS.gene007947.t1 MTR_5g097480 29.397 398 251 10 107 495 167 543 3.79e-43 160
MS.gene007947.t1 MTR_2g082090 31.719 413 241 14 101 495 189 578 1.45e-42 160
MS.gene007947.t1 MTR_1g096030 31.442 423 220 16 109 495 70 458 6.23e-40 150
MS.gene007947.t1 MTR_3g056110 30.623 369 222 10 140 495 194 541 7.47e-40 151
MS.gene007947.t1 MTR_4g102790 30.993 413 222 16 112 496 285 662 1.59e-39 152
MS.gene007947.t1 MTR_5g094450 28.424 387 257 8 116 495 158 531 3.80e-39 149
MS.gene007947.t1 MTR_4g133660 28.244 393 253 8 114 497 172 544 8.31e-38 146
MS.gene007947.t1 MTR_2g026250 27.171 357 228 11 154 497 260 597 5.24e-35 138
MS.gene007947.t1 MTR_2g034250 26.835 395 271 9 109 495 200 584 2.01e-34 137
MS.gene007947.t1 MTR_2g034260 27.681 401 259 14 109 495 200 583 3.59e-33 133
MS.gene007947.t1 MTR_4g064150 25.238 420 282 11 89 496 334 733 2.40e-32 131
MS.gene007947.t1 MTR_2g034280 26.531 392 273 10 109 495 193 574 4.88e-32 129
MS.gene007947.t1 MTR_4g064200 28.362 409 240 17 111 498 276 652 1.11e-31 129
MS.gene007947.t1 MTR_2g097410 26.329 414 258 14 103 496 355 741 1.55e-31 129
MS.gene007947.t1 MTR_7g062120 27.143 420 263 12 93 495 346 739 5.31e-31 127
MS.gene007947.t1 MTR_2g097467 28.032 371 225 14 144 496 308 654 9.12e-30 123
MS.gene007947.t1 MTR_2g099110 29.595 321 196 10 98 409 53 352 4.24e-29 119
MS.gene007947.t1 MTR_4g097080 27.202 386 258 11 118 495 132 502 6.85e-29 120
MS.gene007947.t1 MTR_4g064120 24.311 399 269 11 66 449 201 581 1.11e-28 120
MS.gene007947.t1 MTR_3g022830 24.554 448 258 13 105 495 11 435 3.56e-28 117
MS.gene007947.t1 MTR_0092s0100 28.205 390 243 15 116 497 369 729 4.20e-28 119
MS.gene007947.t1 MTR_0092s0100 28.205 390 243 15 116 497 372 732 4.38e-28 119
MS.gene007947.t1 MTR_4g064160 25.175 429 280 13 84 496 281 684 2.86e-27 116
MS.gene007947.t1 MTR_2g097463 29.936 314 189 12 200 496 356 655 3.06e-27 116
MS.gene007947.t1 MTR_5g015490 27.525 396 250 14 118 495 113 489 1.32e-26 113
MS.gene007947.t1 MTR_4g064180 24.590 427 284 13 87 497 220 624 6.68e-26 112
MS.gene007947.t1 MTR_2g097473 27.933 358 220 13 154 496 320 654 1.41e-25 110
MS.gene007947.t1 MTR_7g109580 25.377 398 263 14 109 495 179 553 3.99e-25 109
MS.gene007947.t1 MTR_2g097390 24.831 443 280 15 71 495 233 640 1.51e-24 107
MS.gene007947.t1 MTR_2g097310 24.605 443 283 15 71 496 230 638 1.35e-23 104
MS.gene007947.t1 MTR_8g077940 28.267 375 227 13 136 497 197 542 2.42e-23 103
MS.gene007947.t1 MTR_4g026485 26.379 417 266 15 86 497 245 625 3.10e-22 100
MS.gene007947.t1 MTR_2g089100 28.037 321 191 10 210 498 133 445 4.13e-22 99.4
MS.gene007947.t1 MTR_2g097350 25.823 395 246 15 119 495 274 639 4.90e-22 100
MS.gene007947.t1 MTR_4g076140 30.043 233 142 7 105 335 34 247 2.14e-21 97.4
MS.gene007947.t1 MTR_4g076140 28.000 150 102 3 349 495 322 468 1.41e-12 70.1
MS.gene007947.t1 MTR_5g009080 28.936 235 144 8 104 335 41 255 5.17e-21 96.3
MS.gene007947.t1 MTR_5g009080 31.646 158 94 5 345 495 327 477 2.46e-13 72.4
MS.gene007947.t1 MTR_2g097380 24.528 371 202 14 148 496 247 561 1.22e-20 95.5
MS.gene007947.t1 MTR_7g069740 26.161 409 253 14 105 495 206 583 1.60e-20 95.1
MS.gene007947.t1 MTR_1g086970 23.500 400 275 12 109 495 96 477 2.66e-19 90.9
MS.gene007947.t1 MTR_8g020840 24.495 396 263 12 118 495 170 547 1.51e-14 76.6
MS.gene007947.t1 MTR_4g095500 23.488 430 257 16 118 495 58 467 5.35e-13 71.2
MS.gene007947.t1 MTR_5g015950 21.564 422 277 10 118 495 34 445 6.07e-13 71.2
MS.gene007947.t1 MTR_3g053270 21.564 422 277 10 118 495 34 445 6.07e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007947.t1 AT4G08250 50.898 501 208 12 11 501 10 482 1.07e-158 460
MS.gene007947.t1 AT3G54220 36.386 415 229 16 94 499 265 653 4.90e-52 187
MS.gene007947.t1 AT5G41920 34.988 403 235 12 102 501 27 405 1.82e-51 180
MS.gene007947.t1 AT1G66350 32.609 414 232 15 90 495 131 505 4.46e-48 174
MS.gene007947.t1 AT3G03450 31.514 403 233 14 107 495 171 544 2.65e-46 169
MS.gene007947.t1 AT5G66770 32.029 409 255 7 93 495 192 583 2.72e-46 170
MS.gene007947.t1 AT5G17490 32.446 413 237 14 98 499 138 519 8.66e-46 167
MS.gene007947.t1 AT3G50650 33.425 365 215 7 141 495 195 541 1.96e-44 164
MS.gene007947.t1 AT1G55580 34.505 313 188 7 196 495 136 444 1.09e-43 160
MS.gene007947.t1 AT5G48150 29.897 388 239 10 120 495 123 489 6.46e-43 159
MS.gene007947.t1 AT5G48150 29.897 388 239 10 120 495 123 489 6.46e-43 159
MS.gene007947.t1 AT5G48150 29.897 388 239 10 120 495 123 489 6.46e-43 159
MS.gene007947.t1 AT5G48150 29.897 388 239 10 120 495 123 489 6.46e-43 159
MS.gene007947.t1 AT2G01570 31.204 407 234 14 107 500 212 585 1.45e-41 157
MS.gene007947.t1 AT4G17230 31.608 367 213 10 144 495 181 524 8.15e-41 154
MS.gene007947.t1 AT4G17230 31.608 367 213 10 144 495 181 524 8.15e-41 154
MS.gene007947.t1 AT1G21450 31.202 391 234 14 118 495 224 592 6.25e-40 152
MS.gene007947.t1 AT1G14920 29.756 410 236 16 107 500 160 533 3.92e-39 149
MS.gene007947.t1 AT2G04890 31.156 398 230 14 111 495 46 412 4.62e-39 147
MS.gene007947.t1 AT1G50600 30.789 380 229 12 130 495 166 525 8.36e-38 145
MS.gene007947.t1 AT1G50600 30.789 380 229 12 130 495 241 600 1.86e-37 145
MS.gene007947.t1 AT1G50600 30.789 380 229 12 130 495 237 596 1.88e-37 145
MS.gene007947.t1 AT1G07530 27.938 451 281 13 65 500 348 769 2.76e-37 146
MS.gene007947.t1 AT1G63100 29.078 423 262 13 93 496 226 629 1.95e-36 143
MS.gene007947.t1 AT1G63100 29.078 423 262 13 93 496 251 654 2.71e-36 143
MS.gene007947.t1 AT2G29065 26.598 391 260 10 119 496 252 628 2.87e-32 130
MS.gene007947.t1 AT2G29060 25.745 470 290 17 51 496 221 655 8.78e-32 129
MS.gene007947.t1 AT2G29060 25.745 470 290 17 51 496 258 692 9.51e-32 129
MS.gene007947.t1 AT1G07520 27.222 360 234 9 154 498 350 696 3.28e-31 128
MS.gene007947.t1 AT1G07520 27.222 360 234 9 154 498 349 695 3.33e-31 128
MS.gene007947.t1 AT1G07520 27.222 360 234 9 154 498 185 531 3.33e-31 127
MS.gene007947.t1 AT1G07520 27.222 360 234 9 154 498 185 531 3.33e-31 127
MS.gene007947.t1 AT3G46600 27.638 398 238 12 118 495 3 370 5.66e-31 124
MS.gene007947.t1 AT3G46600 25.778 450 284 12 66 495 159 578 6.49e-31 126
MS.gene007947.t1 AT3G46600 25.778 450 284 12 66 495 127 546 1.10e-30 125
MS.gene007947.t1 AT4G37650 27.820 399 250 13 119 495 146 528 7.14e-30 123
MS.gene007947.t1 AT5G59450 25.000 452 293 12 68 495 168 597 1.77e-29 122
MS.gene007947.t1 AT3G60630 27.970 404 257 12 110 497 238 623 1.59e-28 119
MS.gene007947.t1 AT2G37650 28.321 399 251 13 114 500 330 705 6.95e-27 115
MS.gene007947.t1 AT2G37650 28.321 399 251 13 114 500 342 717 8.00e-27 115
MS.gene007947.t1 AT2G45160 27.251 411 246 14 108 497 262 640 3.83e-26 112
MS.gene007947.t1 AT4G36710 27.913 369 233 12 136 495 140 484 4.68e-26 111
MS.gene007947.t1 AT4G00150 27.436 390 234 13 120 500 209 558 8.06e-21 96.3
MS.gene007947.t1 AT1G50420 32.203 236 135 9 105 335 43 258 2.74e-20 94.0
MS.gene007947.t1 AT1G50420 34.641 153 94 3 348 497 331 480 7.07e-18 86.7
MS.gene007947.t1 AT3G49950 25.469 373 225 13 153 496 60 408 2.13e-19 90.9
MS.gene007947.t1 AT5G52510 28.793 323 188 10 202 495 330 639 5.13e-18 87.8

Find 193 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGGTGAGGTAGGGTTTA+GGG 0.226810 3.4:-71558786 MS.gene007947:CDS
CCTACTTCCGACGATGATTA+TGG 0.250428 3.4:-71560030 MS.gene007947:CDS
GTCAACTGGGACACCTTTAC+AGG 0.297676 3.4:-71559979 MS.gene007947:CDS
GCAGAACCGGGCCCGGGCTT+TGG 0.319278 3.4:-71558903 MS.gene007947:CDS
CATTTCACCGAAGCCCTTCA+TGG 0.325802 3.4:-71559613 MS.gene007947:CDS
TGTCAAGCAAAGCTGTTGTT+AGG 0.326046 3.4:-71558737 MS.gene007947:CDS
TGGGTTTGTGGAAAGGTTCA+TGG 0.326737 3.4:-71558981 MS.gene007947:CDS
TCCACCAACGTCACAAGCTT+TGG 0.330745 3.4:+71559028 None:intergenic
GGTTAGGTGAGGTAGGGTTT+AGG 0.331084 3.4:-71558787 MS.gene007947:CDS
ACTGGTCTCCCATCGTCAAC+TGG 0.335690 3.4:-71559993 MS.gene007947:CDS
AGCTTCGATGATGGCTTGAT+TGG 0.338111 3.4:+71559435 None:intergenic
GGTGGTGGTGGTTGTGGTGA+TGG 0.340117 3.4:+71559879 None:intergenic
CTGGGTTTCCGATGCAGAAC+CGG 0.348964 3.4:-71558916 MS.gene007947:CDS
GGAGAGTGGTTAGGTGAGGT+AGG 0.352680 3.4:-71558794 MS.gene007947:CDS
GTGGTGGTTGTGGTGATGGA+TGG 0.363760 3.4:+71559883 None:intergenic
ATCAATGACCTCCACTTCTC+TGG 0.368955 3.4:-71560075 MS.gene007947:CDS
ACCTCCTTCGCCGCTTCCCT+TGG 0.370284 3.4:-71559229 MS.gene007947:CDS
CCTAGGATTGCAGGCTCGTT+GGG 0.371680 3.4:-71558860 MS.gene007947:CDS
GGTTGTGGTGGTGGTGGTTG+TGG 0.377950 3.4:+71559873 None:intergenic
TTCGGTAGTTGGTGGGTTTG+TGG 0.382194 3.4:-71558993 MS.gene007947:CDS
GCTTGTACGTGGAGAGGCTT+TGG 0.384610 3.4:-71559140 MS.gene007947:CDS
CTTCCGCGCCAGCCATCAAT+AGG 0.391873 3.4:+71559746 None:intergenic
GTTTGCTTTCTGCTTCCCTT+TGG 0.394498 3.4:-71558637 MS.gene007947:CDS
TTCCGATGCAGAACCGGGCC+CGG 0.397897 3.4:-71558910 MS.gene007947:CDS
ATGAGGTGGTGAAAGTCATC+TGG 0.398824 3.4:+71559952 None:intergenic
GCCTAGGATTGCAGGCTCGT+TGG 0.400453 3.4:-71558861 MS.gene007947:CDS
GAAGAAGTTGGTTCGGTAGT+TGG 0.401602 3.4:-71559004 MS.gene007947:CDS
GGTAGGGTTTAGGGGAGTTC+CGG 0.404698 3.4:-71558777 MS.gene007947:CDS
TTCTTCCTCATCGGTGGTTG+TGG 0.404871 3.4:+71559858 None:intergenic
TAGTTACCGTGCACCGGAAT+CGG 0.409078 3.4:-71559086 MS.gene007947:CDS
CGATGAGGAAGAAGAAGAAA+TGG 0.410332 3.4:-71559848 MS.gene007947:CDS
AAGCAGCAAGTGTGTCGTTT+TGG 0.410870 3.4:+71559509 None:intergenic
CATTTCTTCTTCTTCCTCAT+CGG 0.412349 3.4:+71559849 None:intergenic
TCCGATGCAGAACCGGGCCC+GGG 0.417300 3.4:-71558909 MS.gene007947:CDS
GAACCGGGCCCGGGCTTTGG+TGG 0.417856 3.4:-71558900 MS.gene007947:CDS
TGACATGGACGCGGCGTTCG+TGG 0.420981 3.4:+71559407 None:intergenic
AAGAATGAAGCAACCGATTC+CGG 0.425966 3.4:+71559073 None:intergenic
CTCATCGGTGGTTGTGGTGG+TGG 0.431811 3.4:+71559864 None:intergenic
GCATTATCACGGACAGGAAC+CGG 0.434140 3.4:-71559295 MS.gene007947:CDS
CCCAACGAGCCTGCAATCCT+AGG 0.435603 3.4:+71558860 None:intergenic
CAGGGTTGAAGAGGTGGGTG+TGG 0.435647 3.4:-71558696 MS.gene007947:CDS
CATAATCATCGTCGGAAGTA+GGG 0.435805 3.4:+71560031 None:intergenic
TCGCCTGCCGGTTTCTTGTA+CGG 0.437235 3.4:+71559255 None:intergenic
CACCACCCGTTCTATATATC+CGG 0.439796 3.4:+71558837 None:intergenic
GTAATAAGTTGGTGTTGGAT+TGG 0.441173 3.4:-71558670 MS.gene007947:CDS
GCCCTTCATGGACTACTAGA+AGG 0.443992 3.4:-71559601 MS.gene007947:CDS
GAAGTTGGTGATGATTCAAA+AGG 0.445291 3.4:-71559784 MS.gene007947:CDS
ATGTCACCTTATGTCAAATT+CGG 0.447667 3.4:-71559469 MS.gene007947:CDS
AGAGAAGGTCGTGGGGAGAG+TGG 0.453369 3.4:-71558808 MS.gene007947:CDS
GAGAGTGGTTAGGTGAGGTA+GGG 0.454793 3.4:-71558793 MS.gene007947:CDS
TGAGGTAATGTTTGTTGTTG+TGG 0.455641 3.4:+71559554 None:intergenic
AAAACTAGTGCACCTATTGA+TGG 0.457233 3.4:-71559758 MS.gene007947:CDS
GATGGGAGACCAGTGGTTCC+AGG 0.457583 3.4:+71560002 None:intergenic
AGAGAAGTGGAGGTCATTGA+TGG 0.464377 3.4:+71560077 None:intergenic
AAACATTACCTCACCACAAA+TGG 0.466163 3.4:-71559544 MS.gene007947:CDS
TGAAGAGGAGAGAAGGTCGT+GGG 0.469399 3.4:-71558816 MS.gene007947:CDS
GTGGGCCACAGCTTCGATGA+TGG 0.470189 3.4:+71559426 None:intergenic
CCATAATCATCGTCGGAAGT+AGG 0.475182 3.4:+71560030 None:intergenic
AAGATGAGGACCGTTGTTGT+TGG 0.475317 3.4:+71559327 None:intergenic
AGGTCGTGGGGAGAGTGGTT+AGG 0.475697 3.4:-71558803 MS.gene007947:CDS
CATGGACTACTAGAAGGTGC+TGG 0.477897 3.4:-71559595 MS.gene007947:CDS
TCTCCACCAAAGCCCGGGCC+CGG 0.479183 3.4:+71558897 None:intergenic
ACAACAACGGTCCTCATCTT+CGG 0.479785 3.4:-71559324 MS.gene007947:CDS
CGGAAGTAGGGGTGTTGGTG+AGG 0.483268 3.4:+71560043 None:intergenic
GGCCCGGGCTTTGGTGGAGA+GGG 0.485841 3.4:-71558894 MS.gene007947:CDS
ATACCCTCTCCACCAAAGCC+CGG 0.489626 3.4:+71558891 None:intergenic
GTCAAGCAAAGCTGTTGTTA+GGG 0.489648 3.4:-71558736 MS.gene007947:CDS
GCTGGCGCGGAAGCCTTAAC+CGG 0.490918 3.4:-71559736 MS.gene007947:CDS
ATCGCCACCGTACAAGAAAC+CGG 0.491760 3.4:-71559262 MS.gene007947:CDS
TAGTAGTCCATGAAGGGCTT+CGG 0.495192 3.4:+71559606 None:intergenic
ATGGTTGCACCTGGAACCAC+TGG 0.499209 3.4:-71560011 MS.gene007947:CDS
TGGGTACAGGGTTGAAGAGG+TGG 0.499564 3.4:-71558702 MS.gene007947:CDS
ATCGGTGGTTGTGGTGGTGG+TGG 0.499565 3.4:+71559867 None:intergenic
TAGTTGGTGGGTTTGTGGAA+AGG 0.499576 3.4:-71558988 MS.gene007947:CDS
ATTCCGGTGCACGGTAACTA+AGG 0.501357 3.4:+71559089 None:intergenic
TTCTGTCGTTGTTGTTGTTG+TGG 0.501931 3.4:+71559816 None:intergenic
GTCATCTGGGGCACCTGTAA+AGG 0.502615 3.4:+71559966 None:intergenic
TTGTCCAAGGGAAGCGGCGA+AGG 0.503279 3.4:+71559225 None:intergenic
GACATGGACGCGGCGTTCGT+GGG 0.503853 3.4:+71559408 None:intergenic
GTGACATGTCTTGAAGTAGT+TGG 0.504360 3.4:+71559485 None:intergenic
GGAAGTAGGGGTGTTGGTGA+GGG 0.508441 3.4:+71560044 None:intergenic
TGGGTTTCCGATGCAGAACC+GGG 0.509255 3.4:-71558915 MS.gene007947:CDS
CATCATGGAAGGGGTCCAAT+GGG 0.510759 3.4:-71559374 MS.gene007947:CDS
CACCTTCTAGTAGTCCATGA+AGG 0.513198 3.4:+71559599 None:intergenic
TTCTTCTTCTTCCTCATCGG+TGG 0.515776 3.4:+71559852 None:intergenic
TGTTGTTGGAAGCTAGTGAT+TGG 0.517924 3.4:+71559341 None:intergenic
GGATTGCAGGCTCGTTGGGC+CGG 0.518025 3.4:-71558856 MS.gene007947:CDS
GGGTACAGGGTTGAAGAGGT+GGG 0.519826 3.4:-71558701 MS.gene007947:CDS
GGGCCCGGGCTTTGGTGGAG+AGG 0.520373 3.4:-71558895 MS.gene007947:CDS
ATGGACTACTAGAAGGTGCT+GGG 0.522101 3.4:-71559594 MS.gene007947:CDS
AGGGTTGAAGAGGTGGGTGT+GGG 0.522507 3.4:-71558695 MS.gene007947:CDS
GTGAAGAGGAGAGAAGGTCG+TGG 0.522620 3.4:-71558817 MS.gene007947:CDS
GTACGGTGGCGATTGACCTC+CGG 0.522939 3.4:+71559272 None:intergenic
TACCCTCTCCACCAAAGCCC+GGG 0.525259 3.4:+71558892 None:intergenic
ATATAGAACGGGTGGTGAAG+AGG 0.526637 3.4:-71558831 MS.gene007947:CDS
CTGCAGTGGTGGAAAGAAAA+CGG 0.528193 3.4:+71559202 None:intergenic
ACATCATGGAAGGGGTCCAA+TGG 0.528843 3.4:-71559375 MS.gene007947:CDS
TTAGGTGAGGTAGGGTTTAG+GGG 0.528886 3.4:-71558785 MS.gene007947:CDS
CTAGTGCACCTATTGATGGC+TGG 0.529907 3.4:-71559754 MS.gene007947:CDS
GGGAGCTGTGTCCAGAGAAG+TGG 0.530921 3.4:+71560064 None:intergenic
GTTGTCATGGGGACCATTTG+TGG 0.530976 3.4:+71559531 None:intergenic
GGGTAGTAATAAGTTGGTGT+TGG 0.531656 3.4:-71558675 MS.gene007947:CDS
CAGTAATACCATGGACATGA+TGG 0.531857 3.4:-71560109 None:intergenic
AAGTTGGTTCGGTAGTTGGT+GGG 0.534092 3.4:-71559000 MS.gene007947:CDS
GGTGGAAGAAGAAGTTGGTT+CGG 0.535127 3.4:-71559011 MS.gene007947:CDS
TGAAGAAGAACAAGTCCAAA+GGG 0.536908 3.4:+71558622 None:intergenic
GAAGTTGGTTCGGTAGTTGG+TGG 0.538694 3.4:-71559001 MS.gene007947:CDS
CTGGTCTCCCATCGTCAACT+GGG 0.539421 3.4:-71559992 MS.gene007947:CDS
CCTGCCGGTTTCTTGTACGG+TGG 0.541192 3.4:+71559258 None:intergenic
ACATGCTAACGGCTCTAACA+TGG 0.542095 3.4:-71559650 MS.gene007947:CDS
CAATGATTTGCAAAGCTCAC+CGG 0.544682 3.4:+71558758 None:intergenic
GCCCGGGCCCGGTTCTGCAT+CGG 0.547611 3.4:+71558908 None:intergenic
GGGTGTGGGTAGTAATAAGT+TGG 0.548725 3.4:-71558681 MS.gene007947:CDS
GTGAGACTAATTCCTTGAGC+CGG 0.552046 3.4:+71559674 None:intergenic
TGATGGGTACAGGGTTGAAG+AGG 0.552091 3.4:-71558705 MS.gene007947:CDS
CGGGTGGTGAAGAGGAGAGA+AGG 0.553224 3.4:-71558823 MS.gene007947:CDS
TGTCATCGACTACGACATCA+TGG 0.554847 3.4:-71559389 MS.gene007947:CDS
TGAGGTGGTGAAAGTCATCT+GGG 0.555233 3.4:+71559953 None:intergenic
TAGCTCGAGTGATATTGATC+CGG 0.555972 3.4:-71559693 MS.gene007947:CDS
TCGGTTGCTTCATTCTTGAA+CGG 0.556699 3.4:-71559067 MS.gene007947:CDS
TTCCTCATCGGTGGTTGTGG+TGG 0.557874 3.4:+71559861 None:intergenic
ATCGTCGGAAGTAGGGGTGT+TGG 0.558472 3.4:+71560038 None:intergenic
ATCCATGTCCATCATGTCCA+TGG 0.558666 3.4:+71560101 None:intergenic
GGAAAGAAAACGGTTGTCCA+AGG 0.560068 3.4:+71559212 None:intergenic
GAAGAGGAGAGAAGGTCGTG+GGG 0.560976 3.4:-71558815 MS.gene007947:CDS
TTCGGATAACCGCATTATCA+CGG 0.561276 3.4:-71559306 MS.gene007947:CDS
GGTAATGTTTGTTGTTGTGG+TGG 0.561567 3.4:+71559557 None:intergenic
AGATGACTTTCACCACCTCA+TGG 0.561702 3.4:-71559950 MS.gene007947:CDS
CTGAGTCTAACCTGCAGTGG+TGG 0.569298 3.4:+71559191 None:intergenic
TTGGGCCGGATATATAGAAC+GGG 0.569905 3.4:-71558842 MS.gene007947:CDS
AAGTGGCCGAATTTGACATA+AGG 0.570280 3.4:+71559463 None:intergenic
ATTGGATTAATGAAGCCCAT+TGG 0.571351 3.4:+71559359 None:intergenic
AAACGGTTGTCCAAGGGAAG+CGG 0.571859 3.4:+71559219 None:intergenic
TCACCTTAGTTACCGTGCAC+CGG 0.573305 3.4:-71559092 MS.gene007947:CDS
ATCGACTACGACATCATGGA+AGG 0.578928 3.4:-71559385 MS.gene007947:CDS
CCACCGTACAAGAAACCGGC+AGG 0.581224 3.4:-71559258 MS.gene007947:CDS
TCCAAGGGAAGCGGCGAAGG+AGG 0.582127 3.4:+71559228 None:intergenic
ACGATGATTATGGTTGCACC+TGG 0.582304 3.4:-71560020 MS.gene007947:CDS
AGTGATATTGATCCGGCTCA+AGG 0.583439 3.4:-71559686 MS.gene007947:CDS
CTGAAGAAGAACAAGTCCAA+AGG 0.584472 3.4:+71558621 None:intergenic
AGCTGTGTCCAGAGAAGTGG+AGG 0.585513 3.4:+71560067 None:intergenic
AAGGTGTCCCAGTTGACGAT+GGG 0.586507 3.4:+71559985 None:intergenic
CATCTGAGTCTAACCTGCAG+TGG 0.589878 3.4:+71559188 None:intergenic
AAGTTGGTGATGATTCAAAA+GGG 0.593257 3.4:-71559783 MS.gene007947:CDS
GAAGGAGGTTAATCGCCTGC+CGG 0.597149 3.4:+71559243 None:intergenic
CGACTACGACATCATGGAAG+GGG 0.598773 3.4:-71559383 MS.gene007947:CDS
ACCTTCTAGTAGTCCATGAA+GGG 0.600557 3.4:+71559600 None:intergenic
CTCACTTCATCATTATTCAG+CGG 0.602060 3.4:-71558957 MS.gene007947:CDS
GTGGGGAGAGTGGTTAGGTG+AGG 0.605688 3.4:-71558798 MS.gene007947:CDS
TCCAAAGCTTGTGACGTTGG+TGG 0.608726 3.4:-71559029 MS.gene007947:CDS
GCACCTATTGATGGCTGGCG+CGG 0.609141 3.4:-71559749 MS.gene007947:CDS
ATTAAAGCTTGTACGTGGAG+AGG 0.611742 3.4:-71559146 MS.gene007947:CDS
ATAATCATCGTCGGAAGTAG+GGG 0.612654 3.4:+71560032 None:intergenic
TCAAGCCATCATCGAAGCTG+TGG 0.613643 3.4:-71559431 MS.gene007947:CDS
AAGCAACCGATTCCGGTGCA+CGG 0.622561 3.4:+71559080 None:intergenic
CGTACAAGCTTTAATGCAGA+AGG 0.623940 3.4:+71559154 None:intergenic
TTAGTCTCACAACATGCTAA+CGG 0.625561 3.4:-71559661 MS.gene007947:CDS
CACGGACAGGAACCGGCCGG+AGG 0.629234 3.4:-71559288 MS.gene007947:CDS
AATGCGGTTATCCGAAGATG+AGG 0.632509 3.4:+71559313 None:intergenic
AAAGGTGTCCCAGTTGACGA+TGG 0.634828 3.4:+71559984 None:intergenic
TGGATGGACTAAGCTGGTCG+AGG 0.635528 3.4:+71559899 None:intergenic
GTTGGGCCGGATATATAGAA+CGG 0.635781 3.4:-71558843 MS.gene007947:CDS
GGTTCCTGTCCGTGATAATG+CGG 0.642931 3.4:+71559297 None:intergenic
CGATGATTGTGTCCATGAGG+TGG 0.643118 3.4:+71559938 None:intergenic
TCGACTACGACATCATGGAA+GGG 0.644449 3.4:-71559384 MS.gene007947:CDS
GAATCCAAAGCTTGTGACGT+TGG 0.644734 3.4:-71559032 MS.gene007947:CDS
CACCACCACAACCACCGATG+AGG 0.645241 3.4:-71559863 MS.gene007947:CDS
ATAACCGCATTATCACGGAC+AGG 0.646058 3.4:-71559301 MS.gene007947:CDS
TATCACGGACAGGAACCGGC+CGG 0.647005 3.4:-71559291 MS.gene007947:CDS
TGATGTCGTAGTCGATGACA+TGG 0.647412 3.4:+71559392 None:intergenic
ATATCACTCGAGCTAAGTCA+CGG 0.648503 3.4:+71559701 None:intergenic
TGGCTTGATTGGCTGTGAAG+TGG 0.648617 3.4:+71559446 None:intergenic
ACTAAGCTGGTCGAGGACAG+TGG 0.650840 3.4:+71559906 None:intergenic
GAAAGAAAACGGTTGTCCAA+GGG 0.651213 3.4:+71559213 None:intergenic
TGGACTACTAGAAGGTGCTG+GGG 0.652604 3.4:-71559593 MS.gene007947:CDS
CATGGGGACCATTTGTGGTG+AGG 0.653840 3.4:+71559536 None:intergenic
AAGGGCTTCGGTGAAATGAG+CGG 0.654823 3.4:+71559618 None:intergenic
TCTGCATTAAAGCTTGTACG+TGG 0.657776 3.4:-71559151 MS.gene007947:CDS
CGTAGTCGATGACATGGACG+CGG 0.658711 3.4:+71559398 None:intergenic
TACCATGGACATGATGGACA+TGG 0.658713 3.4:-71560103 None:intergenic
TGGTGATGGATGGACTAAGC+TGG 0.659130 3.4:+71559893 None:intergenic
GGCCGGATATATAGAACGGG+TGG 0.661675 3.4:-71558839 MS.gene007947:CDS
GGTGCAACCATAATCATCGT+CGG 0.661939 3.4:+71560023 None:intergenic
CAAGCTTTAATGCAGAAGGA+CGG 0.671677 3.4:+71559158 None:intergenic
GGACTACTAGAAGGTGCTGG+GGG 0.674008 3.4:-71559592 MS.gene007947:CDS
GGTGGCGATTGACCTCCGGC+CGG 0.683391 3.4:+71559276 None:intergenic
AGTTGACGATGGGAGACCAG+TGG 0.695859 3.4:+71559995 None:intergenic
ACGTTGGTGGAAGAAGAAGT+TGG 0.697366 3.4:-71559016 MS.gene007947:CDS
GGTGCACGGTAACTAAGGTG+AGG 0.709219 3.4:+71559094 None:intergenic
TCACTAGCTTCCAACAACAA+CGG 0.720928 3.4:-71559337 MS.gene007947:CDS
GAGGTGGTGAAAGTCATCTG+GGG 0.725269 3.4:+71559954 None:intergenic
CCTCGATGATTGTGTCCATG+AGG 0.737785 3.4:+71559935 None:intergenic
CCTCATGGACACAATCATCG+AGG 0.768679 3.4:-71559935 MS.gene007947:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAGAAAAAACACAAGAAGT+TGG - chr3.4:71558907-71558926 MS.gene007947:CDS 30.0%
ATGTCACCTTATGTCAAATT+CGG - chr3.4:71559237-71559256 MS.gene007947:CDS 30.0%
! TGTTAGGGCTTTTTAATGAT+GGG - chr3.4:71559985-71560004 MS.gene007947:CDS 30.0%
! TTGTTAGGGCTTTTTAATGA+TGG - chr3.4:71559984-71560003 MS.gene007947:CDS 30.0%
!! AAGTTGGTGATGATTCAAAA+GGG - chr3.4:71558923-71558942 MS.gene007947:CDS 30.0%
AAAACTAGTGCACCTATTGA+TGG - chr3.4:71558948-71558967 MS.gene007947:CDS 35.0%
AAACATTACCTCACCACAAA+TGG - chr3.4:71559162-71559181 MS.gene007947:CDS 35.0%
ATTGGATTAATGAAGCCCAT+TGG + chr3.4:71559350-71559369 None:intergenic 35.0%
CATTTCTTCTTCTTCCTCAT+CGG + chr3.4:71558860-71558879 None:intergenic 35.0%
CTCACTTCATCATTATTCAG+CGG - chr3.4:71559749-71559768 MS.gene007947:CDS 35.0%
TGAAGAAGAACAAGTCCAAA+GGG + chr3.4:71560087-71560106 None:intergenic 35.0%
TTAGTCTCACAACATGCTAA+CGG - chr3.4:71559045-71559064 MS.gene007947:CDS 35.0%
!! GAAGTTGGTGATGATTCAAA+AGG - chr3.4:71558922-71558941 MS.gene007947:CDS 35.0%
!! GTAATAAGTTGGTGTTGGAT+TGG - chr3.4:71560036-71560055 MS.gene007947:CDS 35.0%
!! TGAGGTAATGTTTGTTGTTG+TGG + chr3.4:71559155-71559174 None:intergenic 35.0%
!! TTCAGCGGTTTTTGATTCAT+TGG - chr3.4:71559764-71559783 MS.gene007947:CDS 35.0%
!! TTTTTGATTCATTGGAGGCT+GGG - chr3.4:71559772-71559791 MS.gene007947:CDS 35.0%
!!! GCTTTTTAATGATGGGTACA+GGG - chr3.4:71559992-71560011 MS.gene007947:CDS 35.0%
AAGTGGCCGAATTTGACATA+AGG + chr3.4:71559246-71559265 None:intergenic 40.0%
ACCTTCTAGTAGTCCATGAA+GGG + chr3.4:71559109-71559128 None:intergenic 40.0%
ATAATCATCGTCGGAAGTAG+GGG + chr3.4:71558677-71558696 None:intergenic 40.0%
ATATCACTCGAGCTAAGTCA+CGG + chr3.4:71559008-71559027 None:intergenic 40.0%
ATTAAAGCTTGTACGTGGAG+AGG - chr3.4:71559560-71559579 MS.gene007947:CDS 40.0%
CATAATCATCGTCGGAAGTA+GGG + chr3.4:71558678-71558697 None:intergenic 40.0%
CGATGAGGAAGAAGAAGAAA+TGG - chr3.4:71558858-71558877 MS.gene007947:CDS 40.0%
CTGAAGAAGAACAAGTCCAA+AGG + chr3.4:71560088-71560107 None:intergenic 40.0%
GAAAGAAAACGGTTGTCCAA+GGG + chr3.4:71559496-71559515 None:intergenic 40.0%
GTCAAGCAAAGCTGTTGTTA+GGG - chr3.4:71559970-71559989 MS.gene007947:CDS 40.0%
GTGACATGTCTTGAAGTAGT+TGG + chr3.4:71559224-71559243 None:intergenic 40.0%
TAGCTCGAGTGATATTGATC+CGG - chr3.4:71559013-71559032 MS.gene007947:CDS 40.0%
TCGGTTGCTTCATTCTTGAA+CGG - chr3.4:71559639-71559658 MS.gene007947:CDS 40.0%
TCTGCATTAAAGCTTGTACG+TGG - chr3.4:71559555-71559574 MS.gene007947:CDS 40.0%
TGTCAAGCAAAGCTGTTGTT+AGG - chr3.4:71559969-71559988 MS.gene007947:CDS 40.0%
TGTTGTTGGAAGCTAGTGAT+TGG + chr3.4:71559368-71559387 None:intergenic 40.0%
TTCGGATAACCGCATTATCA+CGG - chr3.4:71559400-71559419 MS.gene007947:CDS 40.0%
! AAGAATGAAGCAACCGATTC+CGG + chr3.4:71559636-71559655 None:intergenic 40.0%
! CAAGCTTTAATGCAGAAGGA+CGG + chr3.4:71559551-71559570 None:intergenic 40.0%
! CAATGATTTGCAAAGCTCAC+CGG + chr3.4:71559951-71559970 None:intergenic 40.0%
! CGTACAAGCTTTAATGCAGA+AGG + chr3.4:71559555-71559574 None:intergenic 40.0%
! TCACTAGCTTCCAACAACAA+CGG - chr3.4:71559369-71559388 MS.gene007947:CDS 40.0%
! TTCTGTCGTTGTTGTTGTTG+TGG + chr3.4:71558893-71558912 None:intergenic 40.0%
!! AGCGGTTTTTGATTCATTGG+AGG - chr3.4:71559767-71559786 MS.gene007947:CDS 40.0%
!! GGGTAGTAATAAGTTGGTGT+TGG - chr3.4:71560031-71560050 MS.gene007947:CDS 40.0%
!! GGTAATGTTTGTTGTTGTGG+TGG + chr3.4:71559152-71559171 None:intergenic 40.0%
!! GTTTTTGATTCATTGGAGGC+TGG - chr3.4:71559771-71559790 MS.gene007947:CDS 40.0%
!!! GGCTTTTTAATGATGGGTAC+AGG - chr3.4:71559991-71560010 MS.gene007947:CDS 40.0%
!!! GTTTTTGGTTGAACCGGTTA+AGG + chr3.4:71558986-71559005 None:intergenic 40.0%
!!! TGGTGGAGAGGGTATTTTTT+GGG - chr3.4:71559823-71559842 MS.gene007947:CDS 40.0%
!!! TGTGTCGTTTTGGTTGTCAT+GGG + chr3.4:71559190-71559209 None:intergenic 40.0%
!!! TTGGTGGAGAGGGTATTTTT+TGG - chr3.4:71559822-71559841 MS.gene007947:CDS 40.0%
AAGATGAGGACCGTTGTTGT+TGG + chr3.4:71559382-71559401 None:intergenic 45.0%
AATGCGGTTATCCGAAGATG+AGG + chr3.4:71559396-71559415 None:intergenic 45.0%
ACAACAACGGTCCTCATCTT+CGG - chr3.4:71559382-71559401 MS.gene007947:CDS 45.0%
ACGATGATTATGGTTGCACC+TGG - chr3.4:71558686-71558705 MS.gene007947:CDS 45.0%
AGAGAAGTGGAGGTCATTGA+TGG + chr3.4:71558632-71558651 None:intergenic 45.0%
AGATGACTTTCACCACCTCA+TGG - chr3.4:71558756-71558775 MS.gene007947:CDS 45.0%
AGTGATATTGATCCGGCTCA+AGG - chr3.4:71559020-71559039 MS.gene007947:CDS 45.0%
ATAACCGCATTATCACGGAC+AGG - chr3.4:71559405-71559424 MS.gene007947:CDS 45.0%
ATATAGAACGGGTGGTGAAG+AGG - chr3.4:71559875-71559894 MS.gene007947:CDS 45.0%
ATCAATGACCTCCACTTCTC+TGG - chr3.4:71558631-71558650 MS.gene007947:CDS 45.0%
ATCGACTACGACATCATGGA+AGG - chr3.4:71559321-71559340 MS.gene007947:CDS 45.0%
ATGAGGTGGTGAAAGTCATC+TGG + chr3.4:71558757-71558776 None:intergenic 45.0%
CACCACCCGTTCTATATATC+CGG + chr3.4:71559872-71559891 None:intergenic 45.0%
CACCTTCTAGTAGTCCATGA+AGG + chr3.4:71559110-71559129 None:intergenic 45.0%
CCATAATCATCGTCGGAAGT+AGG + chr3.4:71558679-71558698 None:intergenic 45.0%
CCTACTTCCGACGATGATTA+TGG - chr3.4:71558676-71558695 MS.gene007947:CDS 45.0%
GAATCCAAAGCTTGTGACGT+TGG - chr3.4:71559674-71559693 MS.gene007947:CDS 45.0%
GGAAAGAAAACGGTTGTCCA+AGG + chr3.4:71559497-71559516 None:intergenic 45.0%
GGGTGTGGGTAGTAATAAGT+TGG - chr3.4:71560025-71560044 MS.gene007947:CDS 45.0%
GGTGCAACCATAATCATCGT+CGG + chr3.4:71558686-71558705 None:intergenic 45.0%
GTGAGACTAATTCCTTGAGC+CGG + chr3.4:71559035-71559054 None:intergenic 45.0%
GTTAGGTGAGGTAGGGTTTA+GGG - chr3.4:71559920-71559939 MS.gene007947:CDS 45.0%
GTTGGGCCGGATATATAGAA+CGG - chr3.4:71559863-71559882 MS.gene007947:CDS 45.0%
GTTTGCTTTCTGCTTCCCTT+TGG - chr3.4:71560069-71560088 MS.gene007947:CDS 45.0%
TAGTAGTCCATGAAGGGCTT+CGG + chr3.4:71559103-71559122 None:intergenic 45.0%
TCGACTACGACATCATGGAA+GGG - chr3.4:71559322-71559341 MS.gene007947:CDS 45.0%
TGAGGTGGTGAAAGTCATCT+GGG + chr3.4:71558756-71558775 None:intergenic 45.0%
TGATGTCGTAGTCGATGACA+TGG + chr3.4:71559317-71559336 None:intergenic 45.0%
TGGGTTTGTGGAAAGGTTCA+TGG - chr3.4:71559725-71559744 MS.gene007947:CDS 45.0%
TTAGGTGAGGTAGGGTTTAG+GGG - chr3.4:71559921-71559940 MS.gene007947:CDS 45.0%
TTCTTCTTCTTCCTCATCGG+TGG + chr3.4:71558857-71558876 None:intergenic 45.0%
TTGGGCCGGATATATAGAAC+GGG - chr3.4:71559864-71559883 MS.gene007947:CDS 45.0%
! ACATGCTAACGGCTCTAACA+TGG - chr3.4:71559056-71559075 MS.gene007947:CDS 45.0%
! ACGTTGGTGGAAGAAGAAGT+TGG - chr3.4:71559690-71559709 MS.gene007947:CDS 45.0%
! AGCTTCGATGATGGCTTGAT+TGG + chr3.4:71559274-71559293 None:intergenic 45.0%
! CTGCAGTGGTGGAAAGAAAA+CGG + chr3.4:71559507-71559526 None:intergenic 45.0%
! GGTGGAAGAAGAAGTTGGTT+CGG - chr3.4:71559695-71559714 MS.gene007947:CDS 45.0%
! TCGAGCTAAGTCACGGTTTT+TGG + chr3.4:71559001-71559020 None:intergenic 45.0%
! TGTCATCGACTACGACATCA+TGG - chr3.4:71559317-71559336 MS.gene007947:CDS 45.0%
!! AAGTTGGTTCGGTAGTTGGT+GGG - chr3.4:71559706-71559725 MS.gene007947:CDS 45.0%
!! ATGGACTACTAGAAGGTGCT+GGG - chr3.4:71559112-71559131 MS.gene007947:CDS 45.0%
!! GAAGAAGTTGGTTCGGTAGT+TGG - chr3.4:71559702-71559721 MS.gene007947:CDS 45.0%
!! TAGTTGGTGGGTTTGTGGAA+AGG - chr3.4:71559718-71559737 MS.gene007947:CDS 45.0%
!!! AAGCAGCAAGTGTGTCGTTT+TGG + chr3.4:71559200-71559219 None:intergenic 45.0%
!!! AGAGGGTATTTTTTGGGCCT+AGG - chr3.4:71559829-71559848 MS.gene007947:CDS 45.0%
!!! GTCACGGTTTTTGGTTGAAC+CGG + chr3.4:71558992-71559011 None:intergenic 45.0%
!!! GTGTCGTTTTGGTTGTCATG+GGG + chr3.4:71559189-71559208 None:intergenic 45.0%
!!! GTGTGTCGTTTTGGTTGTCA+TGG + chr3.4:71559191-71559210 None:intergenic 45.0%
!!! TTTTTTGGGCCTAGGATTGC+AGG - chr3.4:71559837-71559856 MS.gene007947:CDS 45.0%
AAACGGTTGTCCAAGGGAAG+CGG + chr3.4:71559490-71559509 None:intergenic 50.0%
AAGGGCTTCGGTGAAATGAG+CGG + chr3.4:71559091-71559110 None:intergenic 50.0%
ACATCATGGAAGGGGTCCAA+TGG - chr3.4:71559331-71559350 MS.gene007947:CDS 50.0%
ATCGCCACCGTACAAGAAAC+CGG - chr3.4:71559444-71559463 MS.gene007947:CDS 50.0%
ATTCCGGTGCACGGTAACTA+AGG + chr3.4:71559620-71559639 None:intergenic 50.0%
CATCATGGAAGGGGTCCAAT+GGG - chr3.4:71559332-71559351 MS.gene007947:CDS 50.0%
CATCTGAGTCTAACCTGCAG+TGG + chr3.4:71559521-71559540 None:intergenic 50.0%
CATTTCACCGAAGCCCTTCA+TGG - chr3.4:71559093-71559112 MS.gene007947:CDS 50.0%
CCTCATGGACACAATCATCG+AGG - chr3.4:71558771-71558790 MS.gene007947:CDS 50.0%
CCTCGATGATTGTGTCCATG+AGG + chr3.4:71558774-71558793 None:intergenic 50.0%
CGACTACGACATCATGGAAG+GGG - chr3.4:71559323-71559342 MS.gene007947:CDS 50.0%
CGATGATTGTGTCCATGAGG+TGG + chr3.4:71558771-71558790 None:intergenic 50.0%
GAGAGTGGTTAGGTGAGGTA+GGG - chr3.4:71559913-71559932 MS.gene007947:CDS 50.0%
GAGGTGGTGAAAGTCATCTG+GGG + chr3.4:71558755-71558774 None:intergenic 50.0%
GCATTATCACGGACAGGAAC+CGG - chr3.4:71559411-71559430 MS.gene007947:CDS 50.0%
GGTTAGGTGAGGTAGGGTTT+AGG - chr3.4:71559919-71559938 MS.gene007947:CDS 50.0%
GTCAACTGGGACACCTTTAC+AGG - chr3.4:71558727-71558746 MS.gene007947:CDS 50.0%
GTTGTCATGGGGACCATTTG+TGG + chr3.4:71559178-71559197 None:intergenic 50.0%
TAGTTACCGTGCACCGGAAT+CGG - chr3.4:71559620-71559639 MS.gene007947:CDS 50.0%
TCAAGCCATCATCGAAGCTG+TGG - chr3.4:71559275-71559294 MS.gene007947:CDS 50.0%
TCACCTTAGTTACCGTGCAC+CGG - chr3.4:71559614-71559633 MS.gene007947:CDS 50.0%
TCCAAAGCTTGTGACGTTGG+TGG - chr3.4:71559677-71559696 MS.gene007947:CDS 50.0%
TCCACCAACGTCACAAGCTT+TGG + chr3.4:71559681-71559700 None:intergenic 50.0%
TGAAGAGGAGAGAAGGTCGT+GGG - chr3.4:71559890-71559909 MS.gene007947:CDS 50.0%
TTCTTCCTCATCGGTGGTTG+TGG + chr3.4:71558851-71558870 None:intergenic 50.0%
! AAGGTGTCCCAGTTGACGAT+GGG + chr3.4:71558724-71558743 None:intergenic 50.0%
! CATGGACTACTAGAAGGTGC+TGG - chr3.4:71559111-71559130 MS.gene007947:CDS 50.0%
! CGTTTTCTTTCCACCACTGC+AGG - chr3.4:71559505-71559524 MS.gene007947:CDS 50.0%
! CTAGTGCACCTATTGATGGC+TGG - chr3.4:71558952-71558971 MS.gene007947:CDS 50.0%
! GCCCTTCATGGACTACTAGA+AGG - chr3.4:71559105-71559124 MS.gene007947:CDS 50.0%
! GGTTCCTGTCCGTGATAATG+CGG + chr3.4:71559412-71559431 None:intergenic 50.0%
! TGATGGGTACAGGGTTGAAG+AGG - chr3.4:71560001-71560020 MS.gene007947:CDS 50.0%
! TGGCTTGATTGGCTGTGAAG+TGG + chr3.4:71559263-71559282 None:intergenic 50.0%
!! AAAGGTGTCCCAGTTGACGA+TGG + chr3.4:71558725-71558744 None:intergenic 50.0%
!! GAAGTTGGTTCGGTAGTTGG+TGG - chr3.4:71559705-71559724 MS.gene007947:CDS 50.0%
!! TGGACTACTAGAAGGTGCTG+GGG - chr3.4:71559113-71559132 MS.gene007947:CDS 50.0%
!! TGGTGATGGATGGACTAAGC+TGG + chr3.4:71558816-71558835 None:intergenic 50.0%
!! TTCGGTAGTTGGTGGGTTTG+TGG - chr3.4:71559713-71559732 MS.gene007947:CDS 50.0%
ACTAAGCTGGTCGAGGACAG+TGG + chr3.4:71558803-71558822 None:intergenic 55.0%
AGCTGTGTCCAGAGAAGTGG+AGG + chr3.4:71558642-71558661 None:intergenic 55.0%
AGGGTTGAAGAGGTGGGTGT+GGG - chr3.4:71560011-71560030 MS.gene007947:CDS 55.0%
AGTTGACGATGGGAGACCAG+TGG + chr3.4:71558714-71558733 None:intergenic 55.0%
ATACCCTCTCCACCAAAGCC+CGG + chr3.4:71559818-71559837 None:intergenic 55.0%
ATGGTTGCACCTGGAACCAC+TGG - chr3.4:71558695-71558714 MS.gene007947:CDS 55.0%
CATGGGGACCATTTGTGGTG+AGG + chr3.4:71559173-71559192 None:intergenic 55.0%
CCTAGGATTGCAGGCTCGTT+GGG - chr3.4:71559846-71559865 MS.gene007947:CDS 55.0%
CGTAGTCGATGACATGGACG+CGG + chr3.4:71559311-71559330 None:intergenic 55.0%
CTGAGTCTAACCTGCAGTGG+TGG + chr3.4:71559518-71559537 None:intergenic 55.0%
CTGGGTTTCCGATGCAGAAC+CGG - chr3.4:71559790-71559809 MS.gene007947:CDS 55.0%
CTGGTCTCCCATCGTCAACT+GGG - chr3.4:71558714-71558733 MS.gene007947:CDS 55.0%
GAAGAGGAGAGAAGGTCGTG+GGG - chr3.4:71559891-71559910 MS.gene007947:CDS 55.0%
GAAGGAGGTTAATCGCCTGC+CGG + chr3.4:71559466-71559485 None:intergenic 55.0%
GCTTGTACGTGGAGAGGCTT+TGG - chr3.4:71559566-71559585 MS.gene007947:CDS 55.0%
GGAGAGTGGTTAGGTGAGGT+AGG - chr3.4:71559912-71559931 MS.gene007947:CDS 55.0%
GGCCGGATATATAGAACGGG+TGG - chr3.4:71559867-71559886 MS.gene007947:CDS 55.0%
GGGTACAGGGTTGAAGAGGT+GGG - chr3.4:71560005-71560024 MS.gene007947:CDS 55.0%
GGTAGGGTTTAGGGGAGTTC+CGG - chr3.4:71559929-71559948 MS.gene007947:CDS 55.0%
GGTGCACGGTAACTAAGGTG+AGG + chr3.4:71559615-71559634 None:intergenic 55.0%
GTGAAGAGGAGAGAAGGTCG+TGG - chr3.4:71559889-71559908 MS.gene007947:CDS 55.0%
TGGGTACAGGGTTGAAGAGG+TGG - chr3.4:71560004-71560023 MS.gene007947:CDS 55.0%
TGGGTTTCCGATGCAGAACC+GGG - chr3.4:71559791-71559810 MS.gene007947:CDS 55.0%
TTCCTCATCGGTGGTTGTGG+TGG + chr3.4:71558848-71558867 None:intergenic 55.0%
! AAGCAACCGATTCCGGTGCA+CGG + chr3.4:71559629-71559648 None:intergenic 55.0%
! ACTGGTCTCCCATCGTCAAC+TGG - chr3.4:71558713-71558732 MS.gene007947:CDS 55.0%
! ATCGTCGGAAGTAGGGGTGT+TGG + chr3.4:71558671-71558690 None:intergenic 55.0%
! GTCATCTGGGGCACCTGTAA+AGG + chr3.4:71558743-71558762 None:intergenic 55.0%
!! GGAAGTAGGGGTGTTGGTGA+GGG + chr3.4:71558665-71558684 None:intergenic 55.0%
!! GGACTACTAGAAGGTGCTGG+GGG - chr3.4:71559114-71559133 MS.gene007947:CDS 55.0%
!! GTGGTGGTTGTGGTGATGGA+TGG + chr3.4:71558826-71558845 None:intergenic 55.0%
!! TCGCCTGCCGGTTTCTTGTA+CGG + chr3.4:71559454-71559473 None:intergenic 55.0%
!! TGGATGGACTAAGCTGGTCG+AGG + chr3.4:71558810-71558829 None:intergenic 55.0%
AGAGAAGGTCGTGGGGAGAG+TGG - chr3.4:71559898-71559917 MS.gene007947:CDS 60.0%
AGGTCGTGGGGAGAGTGGTT+AGG - chr3.4:71559903-71559922 MS.gene007947:CDS 60.0%
CACCACCACAACCACCGATG+AGG - chr3.4:71558843-71558862 MS.gene007947:CDS 60.0%
CAGGGTTGAAGAGGTGGGTG+TGG - chr3.4:71560010-71560029 MS.gene007947:CDS 60.0%
CCACCGTACAAGAAACCGGC+AGG - chr3.4:71559448-71559467 MS.gene007947:CDS 60.0%
CCCAACGAGCCTGCAATCCT+AGG + chr3.4:71559849-71559868 None:intergenic 60.0%
CGGGTGGTGAAGAGGAGAGA+AGG - chr3.4:71559883-71559902 MS.gene007947:CDS 60.0%
CTCATCGGTGGTTGTGGTGG+TGG + chr3.4:71558845-71558864 None:intergenic 60.0%
CTTCCGCGCCAGCCATCAAT+AGG + chr3.4:71558963-71558982 None:intergenic 60.0%
GATGGGAGACCAGTGGTTCC+AGG + chr3.4:71558707-71558726 None:intergenic 60.0%
GCCTAGGATTGCAGGCTCGT+TGG - chr3.4:71559845-71559864 MS.gene007947:CDS 60.0%
GGGAGCTGTGTCCAGAGAAG+TGG + chr3.4:71558645-71558664 None:intergenic 60.0%
GTACGGTGGCGATTGACCTC+CGG + chr3.4:71559437-71559456 None:intergenic 60.0%
GTGGGCCACAGCTTCGATGA+TGG + chr3.4:71559283-71559302 None:intergenic 60.0%
GTGGGGAGAGTGGTTAGGTG+AGG - chr3.4:71559908-71559927 MS.gene007947:CDS 60.0%
TACCCTCTCCACCAAAGCCC+GGG + chr3.4:71559817-71559836 None:intergenic 60.0%
TATCACGGACAGGAACCGGC+CGG - chr3.4:71559415-71559434 MS.gene007947:CDS 60.0%
TTGTCCAAGGGAAGCGGCGA+AGG + chr3.4:71559484-71559503 None:intergenic 60.0%
! GCACCTATTGATGGCTGGCG+CGG - chr3.4:71558957-71558976 MS.gene007947:CDS 60.0%
!! ATCGGTGGTTGTGGTGGTGG+TGG + chr3.4:71558842-71558861 None:intergenic 60.0%
!! CCTGCCGGTTTCTTGTACGG+TGG + chr3.4:71559451-71559470 None:intergenic 60.0%
!! CGGAAGTAGGGGTGTTGGTG+AGG + chr3.4:71558666-71558685 None:intergenic 60.0%
!! GGTGGTGGTGGTTGTGGTGA+TGG + chr3.4:71558830-71558849 None:intergenic 60.0%
!! GGTTGTGGTGGTGGTGGTTG+TGG + chr3.4:71558836-71558855 None:intergenic 60.0%
ACCTCCTTCGCCGCTTCCCT+TGG - chr3.4:71559477-71559496 MS.gene007947:CDS 65.0%
GACATGGACGCGGCGTTCGT+GGG + chr3.4:71559301-71559320 None:intergenic 65.0%
GCTGGCGCGGAAGCCTTAAC+CGG - chr3.4:71558970-71558989 MS.gene007947:CDS 65.0%
GGATTGCAGGCTCGTTGGGC+CGG - chr3.4:71559850-71559869 MS.gene007947:CDS 65.0%
TCCAAGGGAAGCGGCGAAGG+AGG + chr3.4:71559481-71559500 None:intergenic 65.0%
TGACATGGACGCGGCGTTCG+TGG + chr3.4:71559302-71559321 None:intergenic 65.0%
TTCCGATGCAGAACCGGGCC+CGG - chr3.4:71559796-71559815 MS.gene007947:CDS 65.0%
GGTGGCGATTGACCTCCGGC+CGG + chr3.4:71559433-71559452 None:intergenic 70.0%
TCCGATGCAGAACCGGGCCC+GGG - chr3.4:71559797-71559816 MS.gene007947:CDS 70.0%
TCTCCACCAAAGCCCGGGCC+CGG + chr3.4:71559812-71559831 None:intergenic 70.0%
! GGCCCGGGCTTTGGTGGAGA+GGG - chr3.4:71559812-71559831 MS.gene007947:CDS 70.0%
CACGGACAGGAACCGGCCGG+AGG - chr3.4:71559418-71559437 MS.gene007947:CDS 75.0%
GCAGAACCGGGCCCGGGCTT+TGG - chr3.4:71559803-71559822 MS.gene007947:CDS 75.0%
GCCCGGGCCCGGTTCTGCAT+CGG + chr3.4:71559801-71559820 None:intergenic 75.0%
! GAACCGGGCCCGGGCTTTGG+TGG - chr3.4:71559806-71559825 MS.gene007947:CDS 75.0%
! GGGCCCGGGCTTTGGTGGAG+AGG - chr3.4:71559811-71559830 MS.gene007947:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 71558607 71560121 71558607 ID=MS.gene007947
chr3.4 mRNA 71558607 71560121 71558607 ID=MS.gene007947.t1;Parent=MS.gene007947
chr3.4 exon 71558607 71560121 71558607 ID=MS.gene007947.t1.exon1;Parent=MS.gene007947.t1
chr3.4 CDS 71558607 71560121 71558607 ID=cds.MS.gene007947.t1;Parent=MS.gene007947.t1
Gene Sequence

>MS.gene007947

ATGGACATGATGGACATGGATGCCATCAATGACCTCCACTTCTCTGGACACAGCTCCCTCACCAACACCCCTACTTCCGACGATGATTATGGTTGCACCTGGAACCACTGGTCTCCCATCGTCAACTGGGACACCTTTACAGGTGCCCCAGATGACTTTCACCACCTCATGGACACAATCATCGAGGACAGAACCACTGTCCTCGACCAGCTTAGTCCATCCATCACCACAACCACCACCACCACAACCACCGATGAGGAAGAAGAAGAAATGGAAACAACAACCACAACAACAACAACGACAGAAAAAACACAAGAAGTTGGTGATGATTCAAAAGGGCTAAAACTAGTGCACCTATTGATGGCTGGCGCGGAAGCCTTAACCGGTTCAACCAAAAACCGTGACTTAGCTCGAGTGATATTGATCCGGCTCAAGGAATTAGTCTCACAACATGCTAACGGCTCTAACATGGAGAGACTCGCCGCTCATTTCACCGAAGCCCTTCATGGACTACTAGAAGGTGCTGGGGGTGCGCACAACAACCACCACAACAACAAACATTACCTCACCACAAATGGTCCCCATGACAACCAAAACGACACACTTGCTGCTTTCCAACTACTTCAAGACATGTCACCTTATGTCAAATTCGGCCACTTCACAGCCAATCAAGCCATCATCGAAGCTGTGGCCCACGAACGCCGCGTCCATGTCATCGACTACGACATCATGGAAGGGGTCCAATGGGCTTCATTAATCCAATCACTAGCTTCCAACAACAACGGTCCTCATCTTCGGATAACCGCATTATCACGGACAGGAACCGGCCGGAGGTCAATCGCCACCGTACAAGAAACCGGCAGGCGATTAACCTCCTTCGCCGCTTCCCTTGGACAACCGTTTTCTTTCCACCACTGCAGGTTAGACTCAGATGAAACTTTCCGTCCTTCTGCATTAAAGCTTGTACGTGGAGAGGCTTTGGTTTTCAACTGCATGCTGAATTTACCTCACCTTAGTTACCGTGCACCGGAATCGGTTGCTTCATTCTTGAACGGAGCAAAAACGTTGAATCCAAAGCTTGTGACGTTGGTGGAAGAAGAAGTTGGTTCGGTAGTTGGTGGGTTTGTGGAAAGGTTCATGGACTCACTTCATCATTATTCAGCGGTTTTTGATTCATTGGAGGCTGGGTTTCCGATGCAGAACCGGGCCCGGGCTTTGGTGGAGAGGGTATTTTTTGGGCCTAGGATTGCAGGCTCGTTGGGCCGGATATATAGAACGGGTGGTGAAGAGGAGAGAAGGTCGTGGGGAGAGTGGTTAGGTGAGGTAGGGTTTAGGGGAGTTCCGGTGAGCTTTGCAAATCATTGTCAAGCAAAGCTGTTGTTAGGGCTTTTTAATGATGGGTACAGGGTTGAAGAGGTGGGTGTGGGTAGTAATAAGTTGGTGTTGGATTGGAAATCAAGACGTTTGCTTTCTGCTTCCCTTTGGACTTGTTCTTCTTCAGATTCTGATTTATAG
Protein sequence

>MS.gene007947.t1

MDMMDMDAINDLHFSGHSSLTNTPTSDDDYGCTWNHWSPIVNWDTFTGAPDDFHHLMDTIIEDRTTVLDQLSPSITTTTTTTTTDEEEEEMETTTTTTTTTEKTQEVGDDSKGLKLVHLLMAGAEALTGSTKNRDLARVILIRLKELVSQHANGSNMERLAAHFTEALHGLLEGAGGAHNNHHNNKHYLTTNGPHDNQNDTLAAFQLLQDMSPYVKFGHFTANQAIIEAVAHERRVHVIDYDIMEGVQWASLIQSLASNNNGPHLRITALSRTGTGRRSIATVQETGRRLTSFAASLGQPFSFHHCRLDSDETFRPSALKLVRGEALVFNCMLNLPHLSYRAPESVASFLNGAKTLNPKLVTLVEEEVGSVVGGFVERFMDSLHHYSAVFDSLEAGFPMQNRARALVERVFFGPRIAGSLGRIYRTGGEEERRSWGEWLGEVGFRGVPVSFANHCQAKLLLGLFNDGYRVEEVGVGSNKLVLDWKSRRLLSASLWTCSSSDSDL