AlfalfaGEDB | MS.gene008087

M. sativa cultivar XinJiangDaYe / MS.gene008087

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008087.t1	XP_003600854.2	83.3	216	36	0	1	216	1	216	2.20E-94	355.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008087.t1	Q8S9H7	50.0	80	33	1	92	171	129	201	7.7e-11	68.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008087.t1	G7JAP1	83.3	216	36	0	1	216	1	216	1.6e-94	355.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene008087.t1	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008087.t1	MTR_3g070110	92.593	216	16	0	1	216	1	216	4.05e-141	393
MS.gene008087.t1	MTR_5g081860	41.401	157	78	6	21	171	48	196	1.38e-20	87.4
MS.gene008087.t1	MTR_5g069710	37.209	129	68	2	49	171	80	201	2.25e-17	79.3
MS.gene008087.t1	MTR_5g088010	57.143	77	27	4	92	167	57	128	3.94e-17	77.8
MS.gene008087.t1	MTR_8g063600	33.735	166	94	6	9	167	17	173	1.11e-16	77.0
MS.gene008087.t1	MTR_3g462790	35.583	163	85	4	20	171	63	216	1.12e-16	77.4
MS.gene008087.t1	MTR_5g027570	36.879	141	78	4	38	174	32	165	1.65e-16	75.9
MS.gene008087.t1	MTR_7g067080	40.708	113	53	3	59	171	111	209	7.62e-16	75.1
MS.gene008087.t1	MTR_5g027550	43.023	86	43	2	61	140	37	122	1.35e-15	73.2
MS.gene008087.t1	MTR_5g088060	58.904	73	24	4	96	167	273	340	5.43e-15	73.2
MS.gene008087.t1	MTR_5g488160	58.904	73	24	4	96	167	273	340	5.43e-15	73.2
MS.gene008087.t1	MTR_3g104370	38.136	118	57	2	49	166	57	158	6.29e-15	71.2
MS.gene008087.t1	MTR_8g063870	39.007	141	64	6	32	170	42	162	6.91e-15	72.0
MS.gene008087.t1	MTR_1g048660	59.259	54	22	0	87	140	4	57	1.44e-14	67.0
MS.gene008087.t1	MTR_1g111830	32.639	144	79	3	27	170	67	192	1.38e-13	67.8
MS.gene008087.t1	MTR_4g107230	33.333	117	66	2	24	140	25	129	1.40e-13	68.6
MS.gene008087.t1	MTR_2g090305	46.667	75	33	2	94	168	27	94	1.40e-12	64.3
MS.gene008087.t1	MTR_2g100930	39.759	83	43	1	86	168	100	175	1.88e-12	65.9
MS.gene008087.t1	MTR_6g092540	37.692	130	57	6	83	212	87	192	2.93e-12	64.7
MS.gene008087.t1	MTR_4g100630	36.607	112	52	2	59	170	71	163	4.25e-12	64.7
MS.gene008087.t1	MTR_4g111975	44.304	79	37	2	92	170	135	206	5.13e-12	64.3
MS.gene008087.t1	MTR_5g037080	47.368	57	30	0	84	140	91	147	6.68e-12	63.9
MS.gene008087.t1	MTR_4g015130	47.826	69	29	1	103	171	1	62	8.86e-12	59.3
MS.gene008087.t1	MTR_5g075760	45.833	96	43	5	64	158	77	164	1.04e-11	62.4
MS.gene008087.t1	MTR_8g101650	44.000	75	34	1	92	166	102	168	1.72e-11	62.4
MS.gene008087.t1	MTR_0036s0260	45.333	75	34	2	94	168	27	94	3.66e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008087.t1	AT5G05790	41.406	128	63	3	49	172	82	201	6.02e-20	85.9
MS.gene008087.t1	AT5G05790	41.406	128	63	3	49	172	82	201	6.02e-20	85.9
MS.gene008087.t1	AT3G11280	38.217	157	79	7	21	172	54	197	4.91e-19	83.2
MS.gene008087.t1	AT3G11280	38.217	157	79	7	21	172	54	197	4.91e-19	83.2
MS.gene008087.t1	AT5G01200	45.299	117	50	4	56	171	112	215	2.66e-18	81.3
MS.gene008087.t1	AT2G38090	37.069	116	63	2	59	171	102	210	1.79e-16	76.6
MS.gene008087.t1	AT5G58900	44.681	94	45	1	78	171	124	210	4.61e-16	75.5
MS.gene008087.t1	AT5G08520	36.207	174	84	9	12	169	23	185	8.75e-16	74.7
MS.gene008087.t1	AT4G09450	39.837	123	61	4	70	186	66	181	2.98e-14	68.9
MS.gene008087.t1	AT5G04760	49.367	79	33	2	92	170	96	167	1.51e-13	67.4
MS.gene008087.t1	AT1G19000	50.877	57	28	0	84	140	91	147	4.66e-13	67.0
MS.gene008087.t1	AT1G19000	50.877	57	28	0	84	140	91	147	4.66e-13	67.0
MS.gene008087.t1	AT5G47390	35.878	131	66	3	86	216	87	199	7.79e-13	67.0
MS.gene008087.t1	AT1G70000	41.096	73	41	1	70	140	71	143	1.54e-12	65.1
MS.gene008087.t1	AT1G70000	41.096	73	41	1	70	140	71	143	1.54e-12	65.1
MS.gene008087.t1	AT3G10580	46.835	79	33	1	92	170	92	161	2.44e-12	64.7
MS.gene008087.t1	AT3G10580	46.835	79	33	1	92	170	92	161	2.64e-12	64.7
MS.gene008087.t1	AT1G49010	45.570	79	36	2	92	170	131	202	3.07e-12	65.1
MS.gene008087.t1	AT5G56840	52.632	57	27	0	92	148	88	144	2.47e-11	61.6
MS.gene008087.t1	AT5G61620	45.570	79	36	1	92	170	105	176	3.90e-11	61.6
MS.gene008087.t1	AT1G74840	55.102	49	22	0	92	140	93	141	6.29e-11	60.5
MS.gene008087.t1	AT1G74840	55.102	49	22	0	92	140	93	141	6.64e-11	60.8
MS.gene008087.t1	AT3G16350	48.148	54	28	0	87	140	129	182	9.02e-11	60.8

Find 30 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAACCAATAATCATCTTCT+TGG	0.216049	3.3:-61341206	None:intergenic
CATGATCTTTATCACATTCT+TGG	0.225450	3.3:-61341873	None:intergenic
ATTTCCAAGAAGATGATTAT+TGG	0.238910	3.3:+61341202	MS.gene008087:CDS
GACTCATATGCGTGGTTATT+TGG	0.243758	3.3:+61341790	MS.gene008087:CDS
ATAACTACTCTTGATCTTCT+TGG	0.251399	3.3:+61341760	MS.gene008087:CDS
AGTTCTATTGTTATTATTAT+CGG	0.281602	3.3:-61341373	None:intergenic
TTTGAGCATGGCTTGCTACT+TGG	0.356659	3.3:-61341657	None:intergenic
AAAATATCAAAAGGAAACTT+AGG	0.373972	3.3:+61341722	MS.gene008087:CDS
CATTCAAGAAAGTGGTTCAT+TGG	0.387227	3.3:+61341424	MS.gene008087:CDS
AGAATGGTAGAGAATATTCT+AGG	0.388611	3.3:+61341153	None:intergenic
TTGAGCATGGCTTGCTACTT+GGG	0.417939	3.3:-61341656	None:intergenic
TATTTGTAGGCGTTTCTTGA+AGG	0.450413	3.3:+61341564	MS.gene008087:intron
AAATATCAAAAGGAAACTTA+GGG	0.461487	3.3:+61341723	MS.gene008087:CDS
AGATGATTATTGGTTGATGA+AGG	0.467158	3.3:+61341212	MS.gene008087:CDS
AAAGTGGAAAGAAATCTCTA+AGG	0.472931	3.3:+61341609	MS.gene008087:CDS
AAAGAAATCATTCAAGAAAG+TGG	0.476326	3.3:+61341416	MS.gene008087:CDS
ATTTGTAGGCGTTTCTTGAA+GGG	0.494586	3.3:+61341565	MS.gene008087:intron
GATCAAGAGTAGTTATATCA+TGG	0.499404	3.3:-61341753	None:intergenic
TACACGGCAAAGACGGAAAG+TGG	0.504210	3.3:+61341593	MS.gene008087:CDS
GCATGGCTTGCTACTTGGGA+TGG	0.505418	3.3:-61341652	None:intergenic
TGTTGTCGTCTTCAATCACT+TGG	0.511632	3.3:-61341816	None:intergenic
GGACTTCTACACGGCAAAGA+CGG	0.559413	3.3:+61341586	MS.gene008087:CDS
ATACATATGAACATATCAAG+TGG	0.590413	3.3:-61341908	None:intergenic
TTCTTGAAGGGACTTCTACA+CGG	0.594579	3.3:+61341577	MS.gene008087:CDS
GAAGACGACAACAATAACAA+TGG	0.605151	3.3:+61341826	MS.gene008087:CDS
TCAGCACAACATCGATGAAG+TGG	0.609150	3.3:+61341266	MS.gene008087:CDS
TTATTATTATCGGTTGCATG+AGG	0.632701	3.3:-61341363	None:intergenic
GCACAACATCGATGAAGTGG+TGG	0.668571	3.3:+61341269	MS.gene008087:CDS
AATATCAAAAGGAAACTTAG+GGG	0.670754	3.3:+61341724	MS.gene008087:CDS
GAAGTGATGACTCATATGCG+TGG	0.764866	3.3:+61341782	MS.gene008087:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGTTCTATTGTTATTATTAT+CGG	-	chr3.3:61341376-61341395	None:intergenic	15.0%
!!	AAAATATCAAAAGGAAACTT+AGG	+	chr3.3:61341722-61341741	MS.gene008087:CDS	20.0%
!!	AAATATCAAAAGGAAACTTA+GGG	+	chr3.3:61341723-61341742	MS.gene008087:CDS	20.0%
!!	ATGATAGCAAAAATATCAAA+AGG	+	chr3.3:61341713-61341732	MS.gene008087:CDS	20.0%
!!!	TTTCTTGAATGATTTCTTTT+TGG	-	chr3.3:61341415-61341434	None:intergenic	20.0%
!	AAAGAAATCATTCAAGAAAG+TGG	+	chr3.3:61341416-61341435	MS.gene008087:CDS	25.0%
!	AATATCAAAAGGAAACTTAG+GGG	+	chr3.3:61341724-61341743	MS.gene008087:CDS	25.0%
!	ATTTCCAAGAAGATGATTAT+TGG	+	chr3.3:61341202-61341221	MS.gene008087:CDS	25.0%
!	GAAACAATAAACGATGAAAA+AGG	-	chr3.3:61341473-61341492	None:intergenic	25.0%
!!!	GACATGGATTTTTTATTTGT+AGG	+	chr3.3:61341551-61341570	MS.gene008087:intron	25.0%
	AAAGTGGAAAGAAATCTCTA+AGG	+	chr3.3:61341609-61341628	MS.gene008087:CDS	30.0%
	ATAACTACTCTTGATCTTCT+TGG	+	chr3.3:61341760-61341779	MS.gene008087:CDS	30.0%
	CATGATCTTTATCACATTCT+TGG	-	chr3.3:61341876-61341895	None:intergenic	30.0%
	GATCAAGAGTAGTTATATCA+TGG	-	chr3.3:61341756-61341775	None:intergenic	30.0%
	TCAACCAATAATCATCTTCT+TGG	-	chr3.3:61341209-61341228	None:intergenic	30.0%
!	ATGCTCAAAAGTACGAAAAA+AGG	+	chr3.3:61341671-61341690	MS.gene008087:CDS	30.0%
!	TTATTATTATCGGTTGCATG+AGG	-	chr3.3:61341366-61341385	None:intergenic	30.0%
!!	AGATGATTATTGGTTGATGA+AGG	+	chr3.3:61341212-61341231	MS.gene008087:CDS	30.0%
!!!	TTTTTTCGTACTTTTGAGCA+TGG	-	chr3.3:61341672-61341691	None:intergenic	30.0%
	ATTTGTAGGCGTTTCTTGAA+GGG	+	chr3.3:61341565-61341584	MS.gene008087:intron	35.0%
	CATTCAAGAAAGTGGTTCAT+TGG	+	chr3.3:61341424-61341443	MS.gene008087:CDS	35.0%
	GAAGACGACAACAATAACAA+TGG	+	chr3.3:61341826-61341845	MS.gene008087:CDS	35.0%
	TATTTGTAGGCGTTTCTTGA+AGG	+	chr3.3:61341564-61341583	MS.gene008087:intron	35.0%
	GACTCATATGCGTGGTTATT+TGG	+	chr3.3:61341790-61341809	MS.gene008087:CDS	40.0%
	TGTTGTCGTCTTCAATCACT+TGG	-	chr3.3:61341819-61341838	None:intergenic	40.0%
	TTCTTGAAGGGACTTCTACA+CGG	+	chr3.3:61341577-61341596	MS.gene008087:CDS	40.0%
	GAAGTGATGACTCATATGCG+TGG	+	chr3.3:61341782-61341801	MS.gene008087:CDS	45.0%
	TCAGCACAACATCGATGAAG+TGG	+	chr3.3:61341266-61341285	MS.gene008087:CDS	45.0%
!!	TCGACGAGCGTTTCACTTTT+TGG	+	chr3.3:61341309-61341328	MS.gene008087:CDS	45.0%
!!	TTGAGCATGGCTTGCTACTT+GGG	-	chr3.3:61341659-61341678	None:intergenic	45.0%
!!	TTTGAGCATGGCTTGCTACT+TGG	-	chr3.3:61341660-61341679	None:intergenic	45.0%
	GCACAACATCGATGAAGTGG+TGG	+	chr3.3:61341269-61341288	MS.gene008087:CDS	50.0%
	GCGCACACATGTGTTTGACA+TGG	+	chr3.3:61341535-61341554	MS.gene008087:intron	50.0%
	GGACTTCTACACGGCAAAGA+CGG	+	chr3.3:61341586-61341605	MS.gene008087:CDS	50.0%
	TACACGGCAAAGACGGAAAG+TGG	+	chr3.3:61341593-61341612	MS.gene008087:CDS	50.0%
!!	GCATGGCTTGCTACTTGGGA+TGG	-	chr3.3:61341655-61341674	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	61341156	61341915	61341156	ID=MS.gene008087
chr3.3	mRNA	61341156	61341915	61341156	ID=MS.gene008087.t1;Parent=MS.gene008087
chr3.3	exon	61341156	61341463	61341156	ID=MS.gene008087.t1.exon1;Parent=MS.gene008087.t1
chr3.3	CDS	61341156	61341463	61341156	ID=cds.MS.gene008087.t1;Parent=MS.gene008087.t1
chr3.3	exon	61341573	61341915	61341573	ID=MS.gene008087.t1.exon2;Parent=MS.gene008087.t1
chr3.3	CDS	61341573	61341915	61341573	ID=cds.MS.gene008087.t1;Parent=MS.gene008087.t1

Gene Sequence

>MS.gene008087

ATGGTAGAGAATATTCTAGGTTACAGTCTTCATAGCATGATGAATAATTTCCAAGAAGATGATTATTGGTTGATGAAGGAAACTATGCTATGTAGTGTTGATCAAATCAATCAGCACAACATCGATGAAGTGGTGGTAGAAAATAATATTGCATCGACGAGCGTTTCACTTTTTGGATATGATTCAGTTGATTCTTTCAACTTCTCTCCTCATGCAACCGATAATAATAACAATAGAACTACGAATATCGTAGAATCCAAAAAGAAATCATTCAAGAAAGTGGTTCATTGGTCACGTGATGAACACATGTAAGTCCTTTTTCATCGTTTATTGTTTCATGTAGTTTGAATTATATCTCTCTTTATATATACACTCGTGCGCGCACACATGTGTTTGACATGGATTTTTTATTTGTAGGCGTTTCTTGAAGGGACTTCTACACGGCAAAGACGGAAAGTGGAAAGAAATCTCTAAGGACTATGTAAAAACAAAGACTCCATCCCAAGTAGCAAGCCATGCTCAAAAGTACGAAAAAAGGCAAAAGCAAAGACTTGATGATGATAGCAAAAATATCAAAAGGAAACTTAGGGGTAGCATCCATGATATAACTACTCTTGATCTTCTTGGAAGTGATGACTCATATGCGTGGTTATTTGGTGACCAAGTGATTGAAGACGACAACAATAACAATGGATCACTCTCAGTCACAACTTTCAACCAAGAATGTGATAAAGATCATGAAGTTGTTGCTTCCACTTGA

Protein sequence

>MS.gene008087.t1

MVENILGYSLHSMMNNFQEDDYWLMKETMLCSVDQINQHNIDEVVVENNIASTSVSLFGYDSVDSFNFSPHATDNNNNRTTNIVESKKKSFKKVVHWSRDEHMRFLKGLLHGKDGKWKEISKDYVKTKTPSQVASHAQKYEKRQKQRLDDDSKNIKRKLRGSIHDITTLDLLGSDDSYAWLFGDQVIEDDNNNNGSLSVTTFNQECDKDHEVVAST