Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008158.t1 | XP_013460707.1 | 94.2 | 260 | 14 | 1 | 1 | 259 | 1 | 260 | 2.10E-139 | 505 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008158.t1 | O80942 | 50.4 | 113 | 43 | 3 | 33 | 135 | 18 | 127 | 2.5e-16 | 87.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008158.t1 | A0A072UZ72 | 94.2 | 260 | 14 | 1 | 1 | 259 | 1 | 260 | 1.5e-139 | 505.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene008158.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008158.t1 | MTR_3g068095 | 94.231 | 260 | 14 | 1 | 1 | 259 | 1 | 260 | 0.0 | 516 |
| MS.gene008158.t1 | MTR_5g080660 | 47.674 | 258 | 93 | 13 | 8 | 259 | 11 | 232 | 7.47e-52 | 169 |
| MS.gene008158.t1 | MTR_4g021270 | 56.311 | 103 | 31 | 3 | 33 | 121 | 36 | 138 | 1.10e-29 | 114 |
| MS.gene008158.t1 | MTR_5g014400 | 71.930 | 57 | 15 | 1 | 33 | 89 | 59 | 114 | 3.56e-23 | 95.1 |
| MS.gene008158.t1 | MTR_2g076060 | 49.398 | 83 | 39 | 1 | 10 | 89 | 2 | 84 | 2.88e-19 | 84.0 |
| MS.gene008158.t1 | MTR_1g070265 | 80.952 | 42 | 8 | 0 | 49 | 90 | 29 | 70 | 9.56e-18 | 79.0 |
| MS.gene008158.t1 | MTR_7g063220 | 70.000 | 50 | 15 | 0 | 55 | 104 | 24 | 73 | 5.33e-16 | 73.2 |
| MS.gene008158.t1 | MTR_4g064873 | 62.500 | 48 | 18 | 0 | 54 | 101 | 20 | 67 | 1.25e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008158.t1 | AT2G37740 | 75.439 | 57 | 11 | 1 | 33 | 89 | 18 | 71 | 5.12e-24 | 98.6 |
| MS.gene008158.t1 | AT4G17810 | 30.804 | 224 | 113 | 4 | 30 | 253 | 19 | 200 | 8.05e-24 | 95.9 |
| MS.gene008158.t1 | AT5G06070 | 37.603 | 242 | 106 | 10 | 25 | 259 | 22 | 225 | 5.81e-23 | 94.0 |
| MS.gene008158.t1 | AT4G17810 | 69.091 | 55 | 17 | 0 | 30 | 84 | 19 | 73 | 1.03e-22 | 92.0 |
| MS.gene008158.t1 | AT3G23130 | 82.927 | 41 | 7 | 0 | 47 | 87 | 37 | 77 | 4.81e-18 | 80.5 |
| MS.gene008158.t1 | AT2G42410 | 78.049 | 41 | 9 | 0 | 48 | 88 | 40 | 80 | 2.62e-17 | 78.6 |
| MS.gene008158.t1 | AT2G42410 | 78.049 | 41 | 9 | 0 | 48 | 88 | 40 | 80 | 2.62e-17 | 78.6 |
| MS.gene008158.t1 | AT2G42410 | 78.049 | 41 | 9 | 0 | 48 | 88 | 66 | 106 | 2.85e-17 | 79.0 |
| MS.gene008158.t1 | AT3G09290 | 63.636 | 44 | 16 | 0 | 55 | 98 | 33 | 76 | 9.90e-13 | 65.1 |
| MS.gene008158.t1 | AT3G53820 | 68.571 | 35 | 11 | 0 | 55 | 89 | 19 | 53 | 3.43e-11 | 60.1 |
Find 47 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATATTCTTCTTCAATTAGA+TGG | 0.176609 | 3.4:-69154263 | MS.gene008158:CDS |
| TGTTGAAACAAGTTTGTCTA+TGG | 0.217128 | 3.4:-69154151 | MS.gene008158:CDS |
| TTCCCAAGACTTGTTGTTGA+TGG | 0.233876 | 3.4:+69154650 | None:intergenic |
| TTTAGGTCTGCTCAAGCTTT+AGG | 0.265540 | 3.4:-69154537 | MS.gene008158:CDS |
| AATGAAGTTCTAATTCATTA+TGG | 0.326510 | 3.4:+69154457 | None:intergenic |
| GGACAAAATAATTCAATGTT+TGG | 0.329063 | 3.4:-69154129 | MS.gene008158:CDS |
| AGATCCAAGTCTTCTATTCC+AGG | 0.334055 | 3.4:+69153940 | None:intergenic |
| GGATACTTGGTGGATGTATA+TGG | 0.348621 | 3.4:-69154599 | MS.gene008158:CDS |
| GAAGAAATGGAAGCTGTCTT+TGG | 0.357856 | 3.4:+69154045 | None:intergenic |
| CAGTCTGCAGAGTTTGTGGT+TGG | 0.405826 | 3.4:-69153970 | MS.gene008158:CDS |
| TAAATATGATGTGGATGAAA+AGG | 0.412314 | 3.4:-69154719 | MS.gene008158:CDS |
| GACAGGCAGTGAAGAAGAAA+TGG | 0.412947 | 3.4:+69154032 | None:intergenic |
| TCTTGTGTAGATGCAGTTGA+TGG | 0.423703 | 3.4:+69154312 | None:intergenic |
| AATGAAGGAGTGCCATCATT+TGG | 0.429411 | 3.4:-69154675 | MS.gene008158:CDS |
| GAAGAATATGAAGACATGTT+TGG | 0.429891 | 3.4:+69154276 | None:intergenic |
| ATATTCTTCTTCAATTAGAT+GGG | 0.438910 | 3.4:-69154262 | MS.gene008158:CDS |
| GAAGATGCAGCTAGGATACT+TGG | 0.443307 | 3.4:-69154612 | MS.gene008158:CDS |
| GAACCATCAACAACAAGTCT+TGG | 0.447185 | 3.4:-69154653 | MS.gene008158:CDS |
| ACTTCTGTAAAAGAGAGTTT+AGG | 0.451370 | 3.4:-69154554 | MS.gene008158:CDS |
| TTGGATCTTGAACTCAGGCT+TGG | 0.454061 | 3.4:-69153925 | MS.gene008158:CDS |
| TGGATCTTGAACTCAGGCTT+GGG | 0.459255 | 3.4:-69153924 | MS.gene008158:CDS |
| AAACATGGCTTCAGCAAGAC+AGG | 0.470524 | 3.4:+69154015 | None:intergenic |
| AAGTGACATGAGTAAGATCT+TGG | 0.483411 | 3.4:+69154573 | None:intergenic |
| TTTGTGGTTGGAGTTAAGCC+TGG | 0.483977 | 3.4:-69153958 | MS.gene008158:CDS |
| TTGTTGATGGTTCCAAATGA+TGG | 0.490417 | 3.4:+69154663 | None:intergenic |
| AACCATCAACAACAAGTCTT+GGG | 0.510991 | 3.4:-69154652 | MS.gene008158:CDS |
| GTTGAAACAAGTTTGTCTAT+GGG | 0.512192 | 3.4:-69154150 | MS.gene008158:CDS |
| AAGACTTGGATCTTGAACTC+AGG | 0.538252 | 3.4:-69153930 | MS.gene008158:CDS |
| GAGTCTCTTGATTCTGAACA+AGG | 0.543085 | 3.4:-69154225 | MS.gene008158:CDS |
| AGGTCTGCTCAAGCTTTAGG+TGG | 0.544624 | 3.4:-69154534 | MS.gene008158:CDS |
| AACAAGGGCTAGCTAATGCA+AGG | 0.555187 | 3.4:-69154209 | MS.gene008158:CDS |
| TAAGCCTGGAATAGAAGACT+TGG | 0.563435 | 3.4:-69153944 | MS.gene008158:CDS |
| ATCTTGCACTCACACAATGA+AGG | 0.564898 | 3.4:-69154690 | MS.gene008158:CDS |
| GCAGAGATAACTCTTCTACT+TGG | 0.579663 | 3.4:-69154335 | MS.gene008158:CDS |
| TATTCTTCTTCAATTAGATG+GGG | 0.582180 | 3.4:-69154261 | MS.gene008158:CDS |
| GATGCAGCTAGGATACTTGG+TGG | 0.596955 | 3.4:-69154609 | MS.gene008158:CDS |
| TGACATGAGTAAGATCTTGG+AGG | 0.599612 | 3.4:+69154576 | None:intergenic |
| ACTGATGATGATTCATGTGG+TGG | 0.616045 | 3.4:-69154093 | MS.gene008158:CDS |
| CTGATGATGATTCATGTGGT+GGG | 0.621213 | 3.4:-69154092 | MS.gene008158:CDS |
| ATTCTTCTTCAATTAGATGG+GGG | 0.625385 | 3.4:-69154260 | MS.gene008158:CDS |
| AGGAATCACTAAATATGATG+TGG | 0.652487 | 3.4:-69154728 | MS.gene008158:CDS |
| AAACATCTATCATTCAAACA+TGG | 0.662081 | 3.4:+69154000 | None:intergenic |
| TGATGATGATTCATGTGGTG+GGG | 0.671193 | 3.4:-69154091 | MS.gene008158:CDS |
| AGTCTCTTGATTCTGAACAA+GGG | 0.707551 | 3.4:-69154224 | MS.gene008158:CDS |
| ACAAGGGCTAGCTAATGCAA+GGG | 0.711358 | 3.4:-69154208 | MS.gene008158:CDS |
| CAAACTGATGATGATTCATG+TGG | 0.716039 | 3.4:-69154096 | MS.gene008158:CDS |
| CAGAATCAAGAGACTCCATG+TGG | 0.748859 | 3.4:+69154232 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTCTTCTTCAATTAGAT+GGG | - | chr3.4:69154373-69154392 | MS.gene008158:intron | 20.0% |
| !! | CTAATTATTGTTCTAACTTA+TGG | - | chr3.4:69154263-69154282 | MS.gene008158:CDS | 20.0% |
| !!! | AATGAAGTTCTAATTCATTA+TGG | + | chr3.4:69154181-69154200 | None:intergenic | 20.0% |
| ! | AAACATCTATCATTCAAACA+TGG | + | chr3.4:69154638-69154657 | None:intergenic | 25.0% |
| ! | CATATTCTTCTTCAATTAGA+TGG | - | chr3.4:69154372-69154391 | MS.gene008158:intron | 25.0% |
| ! | GGACAAAATAATTCAATGTT+TGG | - | chr3.4:69154506-69154525 | MS.gene008158:CDS | 25.0% |
| ! | TAAATATGATGTGGATGAAA+AGG | - | chr3.4:69153916-69153935 | MS.gene008158:CDS | 25.0% |
| ! | TATTCTTCTTCAATTAGATG+GGG | - | chr3.4:69154374-69154393 | MS.gene008158:intron | 25.0% |
| !! | GAACAAATTTTCAAAGGTTA+TGG | - | chr3.4:69154449-69154468 | MS.gene008158:CDS | 25.0% |
| ACTTCTGTAAAAGAGAGTTT+AGG | - | chr3.4:69154081-69154100 | MS.gene008158:CDS | 30.0% | |
| AGGAATCACTAAATATGATG+TGG | - | chr3.4:69153907-69153926 | MS.gene008158:CDS | 30.0% | |
| ATTCTTCTTCAATTAGATGG+GGG | - | chr3.4:69154375-69154394 | MS.gene008158:intron | 30.0% | |
| GAAGAATATGAAGACATGTT+TGG | + | chr3.4:69154362-69154381 | None:intergenic | 30.0% | |
| GTTGAAACAAGTTTGTCTAT+GGG | - | chr3.4:69154485-69154504 | MS.gene008158:CDS | 30.0% | |
| TGTTGAAACAAGTTTGTCTA+TGG | - | chr3.4:69154484-69154503 | MS.gene008158:CDS | 30.0% | |
| ! | CATCATCAGTTTGCAATTTT+TGG | + | chr3.4:69154532-69154551 | None:intergenic | 30.0% |
| AACCATCAACAACAAGTCTT+GGG | - | chr3.4:69153983-69154002 | MS.gene008158:CDS | 35.0% | |
| AAGTGACATGAGTAAGATCT+TGG | + | chr3.4:69154065-69154084 | None:intergenic | 35.0% | |
| AGTCTCTTGATTCTGAACAA+GGG | - | chr3.4:69154411-69154430 | MS.gene008158:intron | 35.0% | |
| ! | CAAACTGATGATGATTCATG+TGG | - | chr3.4:69154539-69154558 | MS.gene008158:CDS | 35.0% |
| ! | GCAAGGGAACAAATTTTCAA+AGG | - | chr3.4:69154443-69154462 | MS.gene008158:CDS | 35.0% |
| !! | TTGTTGATGGTTCCAAATGA+TGG | + | chr3.4:69153975-69153994 | None:intergenic | 35.0% |
| AAGACTTGGATCTTGAACTC+AGG | - | chr3.4:69154705-69154724 | MS.gene008158:CDS | 40.0% | |
| AGATCCAAGTCTTCTATTCC+AGG | + | chr3.4:69154698-69154717 | None:intergenic | 40.0% | |
| ATCTTGCACTCACACAATGA+AGG | - | chr3.4:69153945-69153964 | MS.gene008158:CDS | 40.0% | |
| GAACCATCAACAACAAGTCT+TGG | - | chr3.4:69153982-69154001 | MS.gene008158:CDS | 40.0% | |
| GAAGAAATGGAAGCTGTCTT+TGG | + | chr3.4:69154593-69154612 | None:intergenic | 40.0% | |
| GAGTCTCTTGATTCTGAACA+AGG | - | chr3.4:69154410-69154429 | MS.gene008158:intron | 40.0% | |
| GCAGAGATAACTCTTCTACT+TGG | - | chr3.4:69154300-69154319 | MS.gene008158:CDS | 40.0% | |
| TAAGCCTGGAATAGAAGACT+TGG | - | chr3.4:69154691-69154710 | MS.gene008158:CDS | 40.0% | |
| TCTTGTGTAGATGCAGTTGA+TGG | + | chr3.4:69154326-69154345 | None:intergenic | 40.0% | |
| TGACATGAGTAAGATCTTGG+AGG | + | chr3.4:69154062-69154081 | None:intergenic | 40.0% | |
| TTTAGGTCTGCTCAAGCTTT+AGG | - | chr3.4:69154098-69154117 | MS.gene008158:CDS | 40.0% | |
| ! | ACTGATGATGATTCATGTGG+TGG | - | chr3.4:69154542-69154561 | MS.gene008158:CDS | 40.0% |
| ! | CTGATGATGATTCATGTGGT+GGG | - | chr3.4:69154543-69154562 | MS.gene008158:CDS | 40.0% |
| ! | GGATACTTGGTGGATGTATA+TGG | - | chr3.4:69154036-69154055 | MS.gene008158:CDS | 40.0% |
| ! | TGATGATGATTCATGTGGTG+GGG | - | chr3.4:69154544-69154563 | MS.gene008158:CDS | 40.0% |
| ! | TTTTCAGTCTGCAGAGTTTG+TGG | - | chr3.4:69154661-69154680 | MS.gene008158:CDS | 40.0% |
| !! | AATGAAGGAGTGCCATCATT+TGG | - | chr3.4:69153960-69153979 | MS.gene008158:CDS | 40.0% |
| !! | TTCCCAAGACTTGTTGTTGA+TGG | + | chr3.4:69153988-69154007 | None:intergenic | 40.0% |
| AAACATGGCTTCAGCAAGAC+AGG | + | chr3.4:69154623-69154642 | None:intergenic | 45.0% | |
| CAGAATCAAGAGACTCCATG+TGG | + | chr3.4:69154406-69154425 | None:intergenic | 45.0% | |
| GAAGATGCAGCTAGGATACT+TGG | - | chr3.4:69154023-69154042 | MS.gene008158:CDS | 45.0% | |
| GACAGGCAGTGAAGAAGAAA+TGG | + | chr3.4:69154606-69154625 | None:intergenic | 45.0% | |
| TAGATGGGGGAATAACCACA+TGG | - | chr3.4:69154388-69154407 | MS.gene008158:intron | 45.0% | |
| TGGATCTTGAACTCAGGCTT+GGG | - | chr3.4:69154711-69154730 | MS.gene008158:CDS | 45.0% | |
| TTGGATCTTGAACTCAGGCT+TGG | - | chr3.4:69154710-69154729 | MS.gene008158:CDS | 45.0% | |
| TTTGTGGTTGGAGTTAAGCC+TGG | - | chr3.4:69154677-69154696 | MS.gene008158:CDS | 45.0% | |
| ! | CTTTTGCAGAAGATGCAGCT+AGG | - | chr3.4:69154015-69154034 | MS.gene008158:CDS | 45.0% |
| !! | AACAAGGGCTAGCTAATGCA+AGG | - | chr3.4:69154426-69154445 | MS.gene008158:intron | 45.0% |
| !! | ACAAGGGCTAGCTAATGCAA+GGG | - | chr3.4:69154427-69154446 | MS.gene008158:intron | 45.0% |
| CAGTCTGCAGAGTTTGTGGT+TGG | - | chr3.4:69154665-69154684 | MS.gene008158:CDS | 50.0% | |
| GATGCAGCTAGGATACTTGG+TGG | - | chr3.4:69154026-69154045 | MS.gene008158:CDS | 50.0% | |
| !! | AGGTCTGCTCAAGCTTTAGG+TGG | - | chr3.4:69154101-69154120 | MS.gene008158:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 69153903 | 69154754 | 69153903 | ID=MS.gene008158 |
| chr3.4 | mRNA | 69153903 | 69154754 | 69153903 | ID=MS.gene008158.t1;Parent=MS.gene008158 |
| chr3.4 | exon | 69154439 | 69154754 | 69154439 | ID=MS.gene008158.t1.exon1;Parent=MS.gene008158.t1 |
| chr3.4 | CDS | 69154439 | 69154754 | 69154439 | ID=cds.MS.gene008158.t1;Parent=MS.gene008158.t1 |
| chr3.4 | exon | 69153903 | 69154366 | 69153903 | ID=MS.gene008158.t1.exon2;Parent=MS.gene008158.t1 |
| chr3.4 | CDS | 69153903 | 69154366 | 69153903 | ID=cds.MS.gene008158.t1;Parent=MS.gene008158.t1 |
| Gene Sequence |
| Protein sequence |
