AlfalfaGEDB | MS.gene008181

M. sativa cultivar XinJiangDaYe / MS.gene008181

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008181.t1	KEH34714.1	88.9	108	12	0	21	128	2	109	2.30E-46	194.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008181.t1	Q9FKK2	57.9	121	49	1	6	124	4	124	8.8e-31	134.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008181.t1	A0A072UY28	88.9	108	12	0	21	128	2	109	1.7e-46	194.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene008181.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008181.t1	MTR_3g067910	88.889	108	12	0	21	128	2	109	1.59e-68	202
MS.gene008181.t1	MTR_5g045560	80.328	122	24	0	1	122	1	122	7.85e-65	197
MS.gene008181.t1	MTR_2g085280	72.656	128	35	0	1	128	1	128	3.43e-63	192
MS.gene008181.t1	MTR_2g085250	72.656	128	35	0	1	128	1	128	3.78e-63	192
MS.gene008181.t1	MTR_3g052870	73.984	123	32	0	1	123	1	123	4.11e-62	189
MS.gene008181.t1	MTR_1g105920	73.984	123	32	0	1	123	1	123	8.12e-62	189
MS.gene008181.t1	MTR_1g077360	72.951	122	33	0	1	122	1	122	4.91e-61	187
MS.gene008181.t1	MTR_8g043650	72.358	123	34	0	1	123	1	123	9.15e-61	186
MS.gene008181.t1	MTR_1g075600	73.984	123	32	0	1	123	1	123	3.66e-60	185
MS.gene008181.t1	MTR_5g055100	73.171	123	33	0	1	123	1	123	1.90e-59	183
MS.gene008181.t1	MTR_1g105910	72.500	120	33	0	1	120	1	120	4.21e-59	182
MS.gene008181.t1	MTR_5g053390	72.093	129	35	1	1	128	1	129	1.85e-58	181
MS.gene008181.t1	MTR_1g106070	69.919	123	37	0	1	123	1	123	2.41e-58	178
MS.gene008181.t1	MTR_2g030740	70.732	123	36	0	1	123	1	123	3.49e-58	180
MS.gene008181.t1	MTR_3g052920	70.732	123	36	0	1	123	1	123	4.10e-58	179
MS.gene008181.t1	MTR_1g108510	69.106	123	38	0	1	123	1	123	3.77e-56	174
MS.gene008181.t1	MTR_1g012570	71.545	123	35	0	1	123	1	123	5.80e-56	174
MS.gene008181.t1	MTR_3g030770	69.106	123	38	0	1	123	1	123	8.44e-56	174
MS.gene008181.t1	MTR_1g108580	67.480	123	40	0	1	123	1	123	4.67e-55	172
MS.gene008181.t1	MTR_2g093190	67.480	123	40	0	1	123	1	123	5.47e-53	166
MS.gene008181.t1	MTR_0121s0100	65.041	123	43	0	1	123	1	123	2.00e-52	162
MS.gene008181.t1	MTR_3g030780	62.602	123	46	0	1	123	1	123	1.18e-51	160
MS.gene008181.t1	MTR_0121s0080	63.415	123	45	0	1	123	1	123	1.21e-48	155
MS.gene008181.t1	MTR_1g105905	66.667	105	35	0	18	122	1	105	2.43e-45	147
MS.gene008181.t1	MTR_3g080940	54.310	116	53	0	9	124	9	124	2.82e-40	133
MS.gene008181.t1	MTR_1g075570	63.208	106	25	1	18	123	1	92	4.22e-38	126
MS.gene008181.t1	MTR_7g062350	52.756	127	59	1	3	128	14	140	6.39e-36	123
MS.gene008181.t1	MTR_3g067915	81.250	64	12	0	60	123	30	93	5.75e-33	111
MS.gene008181.t1	MTR_3g067875	85.714	56	8	0	18	73	1	56	6.37e-28	97.8
MS.gene008181.t1	MTR_1g108500	46.154	117	40	2	3	117	89	184	4.15e-24	94.7
MS.gene008181.t1	MTR_8g086290	49.425	87	37	1	4	90	7	86	9.20e-24	90.5
MS.gene008181.t1	MTR_2g105290	51.948	77	37	0	1	77	4	80	1.09e-23	90.5
MS.gene008181.t1	MTR_3g067870	69.841	63	19	0	16	78	83	145	3.97e-22	85.9
MS.gene008181.t1	MTR_1g047550	50.704	71	35	0	9	79	2	72	1.51e-21	85.1
MS.gene008181.t1	MTR_7g028448	54.545	77	35	0	4	80	7	83	4.09e-21	83.6
MS.gene008181.t1	MTR_4g036915	54.545	77	35	0	4	80	7	83	4.09e-21	83.6
MS.gene008181.t1	MTR_8g079502	50.704	71	35	0	9	79	2	72	4.62e-21	83.6
MS.gene008181.t1	MTR_4g131030	37.069	116	58	2	9	109	2	117	9.48e-21	83.6
MS.gene008181.t1	MTR_8g051580	45.570	79	43	0	9	87	2	80	2.31e-20	82.0
MS.gene008181.t1	MTR_1g054265	45.570	79	43	0	9	87	2	80	1.22e-19	79.7
MS.gene008181.t1	MTR_1g063160	43.038	79	45	0	9	87	2	80	1.36e-19	79.7
MS.gene008181.t1	MTR_8g046350	45.070	71	39	0	9	79	2	72	2.01e-18	76.6
MS.gene008181.t1	MTR_4g127140	53.247	77	36	0	1	77	4	80	4.05e-18	76.3
MS.gene008181.t1	MTR_4g094632	38.679	106	63	1	9	114	2	105	7.74e-18	74.7
MS.gene008181.t1	MTR_1g053070	49.383	81	41	0	9	89	2	82	1.28e-16	73.6
MS.gene008181.t1	MTR_4g102530	46.575	73	39	0	10	82	3	75	1.57e-15	69.7
MS.gene008181.t1	MTR_3g005530	47.059	68	36	0	9	76	2	69	1.79e-15	70.1
MS.gene008181.t1	MTR_4g093970	50.000	66	33	0	10	75	29	94	1.79e-15	70.1
MS.gene008181.t1	MTR_5g066180	46.479	71	38	0	9	79	2	72	2.54e-15	69.7
MS.gene008181.t1	MTR_8g033270	42.857	98	54	1	9	104	2	99	3.09e-15	69.7
MS.gene008181.t1	MTR_0003s0590	50.000	68	34	0	9	76	2	69	8.88e-15	68.6
MS.gene008181.t1	MTR_3g452380	44.595	74	41	0	3	76	11	84	1.37e-14	68.2
MS.gene008181.t1	MTR_7g075870	49.254	67	34	0	10	76	3	69	1.45e-14	67.4
MS.gene008181.t1	MTR_4g094638	47.826	69	36	0	9	77	2	70	1.77e-14	66.6
MS.gene008181.t1	MTR_1g101970	50.000	68	34	0	9	76	2	69	2.16e-14	66.6
MS.gene008181.t1	MTR_6g464720	49.275	69	34	1	9	76	2	70	2.60e-14	67.4
MS.gene008181.t1	MTR_8g087860	47.059	68	36	0	9	76	18	85	2.87e-14	67.0
MS.gene008181.t1	MTR_2g017865	47.059	68	36	0	9	76	17	84	4.18e-14	67.0
MS.gene008181.t1	MTR_2g009890	43.421	76	43	0	9	84	2	77	4.32e-14	67.0
MS.gene008181.t1	MTR_8g033220	46.377	69	37	0	10	78	3	71	6.85e-14	65.9
MS.gene008181.t1	MTR_8g033220	46.377	69	37	0	10	78	3	71	7.40e-14	65.9
MS.gene008181.t1	MTR_7g075870	48.529	68	34	1	10	76	3	70	2.04e-13	64.3
MS.gene008181.t1	MTR_4g084740	46.053	76	39	2	9	83	2	76	2.05e-13	65.5
MS.gene008181.t1	MTR_5g031000	45.588	68	37	0	9	76	2	69	2.61e-13	64.3
MS.gene008181.t1	MTR_4g109830	46.377	69	36	1	9	76	2	70	2.64e-13	64.3
MS.gene008181.t1	MTR_5g031000	45.588	68	37	0	9	76	2	69	2.89e-13	64.3
MS.gene008181.t1	MTR_5g032150	46.269	67	36	0	10	76	3	69	4.05e-13	63.9
MS.gene008181.t1	MTR_3g102570	41.237	97	54	3	9	104	2	96	4.54e-13	64.7
MS.gene008181.t1	MTR_7g016600	47.826	69	35	1	9	76	2	70	4.88e-13	63.9
MS.gene008181.t1	MTR_5g032520	46.269	67	36	0	10	76	3	69	5.40e-13	63.5
MS.gene008181.t1	MTR_5g046790	44.928	69	37	1	9	76	2	70	6.08e-13	63.2
MS.gene008181.t1	MTR_5g046870	47.826	69	35	1	9	76	2	70	6.57e-13	60.8
MS.gene008181.t1	MTR_2g461710	50.000	56	28	0	9	64	2	57	1.81e-12	58.5
MS.gene008181.t1	MTR_8g066260	43.478	69	38	1	9	76	2	70	1.87e-12	62.0
MS.gene008181.t1	MTR_1g038300	45.714	70	36	1	9	76	2	71	2.00e-12	62.0
MS.gene008181.t1	MTR_5g066960	45.763	59	32	0	9	67	2	60	2.75e-12	58.2
MS.gene008181.t1	MTR_7g016630	40.278	72	42	1	9	79	2	73	9.51e-12	60.5
MS.gene008181.t1	MTR_4g109810	49.153	59	30	0	9	67	2	60	1.36e-11	59.7
MS.gene008181.t1	MTR_6g015975	51.786	56	27	0	9	64	2	57	1.90e-11	59.7
MS.gene008181.t1	MTR_8g097090	44.928	69	37	1	9	76	2	70	2.24e-11	59.3
MS.gene008181.t1	MTR_7g075850	44.286	70	37	1	9	76	2	71	2.73e-11	58.9
MS.gene008181.t1	MTR_3g084980	48.333	60	31	0	9	68	2	61	2.74e-11	58.9
MS.gene008181.t1	MTR_3g088615	41.429	70	39	1	9	76	2	71	4.00e-11	57.8
MS.gene008181.t1	MTR_1g029670	48.214	56	29	0	9	64	2	57	4.81e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008181.t1	AT5G60440	57.851	121	49	1	6	124	4	124	1.43e-41	139
MS.gene008181.t1	AT4G36590	55.556	108	43	1	7	109	5	112	2.05e-31	112
MS.gene008181.t1	AT2G24840	47.458	118	56	2	9	124	63	176	2.03e-28	104
MS.gene008181.t1	AT1G65360	49.107	112	55	1	6	117	4	113	2.80e-28	103
MS.gene008181.t1	AT1G01530	50.962	104	49	1	6	109	4	105	9.58e-28	102
MS.gene008181.t1	AT2G34440	39.640	111	66	1	9	119	2	111	1.11e-22	87.8
MS.gene008181.t1	AT3G04100	35.043	117	71	2	6	117	11	127	5.25e-19	79.0
MS.gene008181.t1	AT3G66656	51.471	68	33	0	9	76	2	69	1.60e-18	77.0
MS.gene008181.t1	AT1G72350	50.685	73	34	1	9	81	43	113	1.81e-18	78.2
MS.gene008181.t1	AT2G45650	52.941	68	32	0	9	76	2	69	5.59e-17	74.7
MS.gene008181.t1	AT2G45660	48.529	68	35	0	10	77	3	70	2.80e-16	70.9
MS.gene008181.t1	AT4G22950	44.737	76	42	0	10	85	3	78	4.48e-16	70.5
MS.gene008181.t1	AT2G45660	48.529	68	35	0	10	77	3	70	5.67e-16	71.2
MS.gene008181.t1	AT4G09960	44.156	77	42	1	1	76	19	95	9.74e-16	71.2
MS.gene008181.t1	AT4G22950	49.254	67	34	0	10	76	3	69	1.43e-15	70.1
MS.gene008181.t1	AT4G22950	49.254	67	34	0	10	76	3	69	1.43e-15	70.1
MS.gene008181.t1	AT2G22540	41.346	104	56	1	10	108	3	106	1.60e-15	70.5
MS.gene008181.t1	AT2G22540	41.346	104	56	1	10	108	3	106	1.60e-15	70.5
MS.gene008181.t1	AT4G09960	47.059	68	36	0	9	76	2	69	1.70e-15	70.1
MS.gene008181.t1	AT4G09960	47.059	68	36	0	9	76	2	69	1.96e-15	70.1
MS.gene008181.t1	AT4G09960	45.833	72	39	0	5	76	8	79	1.98e-15	70.1
MS.gene008181.t1	AT4G09960	47.059	68	36	0	9	76	3	70	2.10e-15	70.1
MS.gene008181.t1	AT1G77950	46.053	76	39	2	9	83	2	76	3.24e-15	69.7
MS.gene008181.t1	AT1G77950	46.053	76	39	2	9	83	2	76	3.24e-15	69.7
MS.gene008181.t1	AT1G77950	46.053	76	39	2	9	83	2	76	3.24e-15	69.7
MS.gene008181.t1	AT1G77950	46.053	76	39	2	9	83	2	76	3.24e-15	69.7
MS.gene008181.t1	AT5G13790	48.529	68	35	0	9	76	2	69	3.67e-15	69.7
MS.gene008181.t1	AT5G13790	48.529	68	35	0	9	76	2	69	3.75e-15	70.1
MS.gene008181.t1	AT4G18960	45.333	75	41	0	2	76	11	85	4.11e-15	69.7
MS.gene008181.t1	AT3G61120	51.471	68	33	0	9	76	2	69	4.27e-15	69.3
MS.gene008181.t1	AT4G09960	47.059	68	36	0	9	76	97	164	5.43e-15	70.1
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	5.91e-15	66.2
MS.gene008181.t1	AT5G51870	49.254	67	34	0	10	76	3	69	7.76e-15	68.2
MS.gene008181.t1	AT5G51870	47.887	71	37	0	6	76	22	92	8.05e-15	67.8
MS.gene008181.t1	AT5G51870	49.254	67	34	0	10	76	3	69	8.14e-15	67.4
MS.gene008181.t1	AT3G57390	50.000	68	34	0	9	76	2	69	8.44e-15	68.2
MS.gene008181.t1	AT1G77980	42.045	88	50	1	9	95	2	89	9.09e-15	69.3
MS.gene008181.t1	AT5G51870	49.254	67	34	0	10	76	3	69	9.99e-15	68.2
MS.gene008181.t1	AT5G51860	49.254	67	34	0	10	76	3	69	1.09e-14	67.8
MS.gene008181.t1	AT5G51870	49.254	67	34	0	10	76	13	79	1.21e-14	67.8
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	1.21e-14	66.6
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	1.21e-14	66.6
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	1.21e-14	66.6
MS.gene008181.t1	AT3G57390	50.000	68	34	0	9	76	2	69	1.23e-14	68.2
MS.gene008181.t1	AT4G24540	48.529	68	35	0	9	76	2	69	1.27e-14	67.8
MS.gene008181.t1	AT1G22130	40.217	92	54	1	9	99	2	93	1.39e-14	68.9
MS.gene008181.t1	AT3G57230	45.588	68	37	0	9	76	2	69	1.54e-14	65.9
MS.gene008181.t1	AT5G51860	49.254	67	34	0	10	76	3	69	1.66e-14	67.4
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	2.48e-14	65.9
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	2.48e-14	65.9
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	2.48e-14	65.9
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	2.48e-14	65.9
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	2.48e-14	65.9
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	2.83e-14	66.2
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	2.83e-14	66.2
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	3.07e-14	66.6
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	3.07e-14	66.6
MS.gene008181.t1	AT5G62165	47.761	67	35	0	10	76	3	69	3.07e-14	66.6
MS.gene008181.t1	AT2G42830	47.059	68	36	0	9	76	17	84	3.90e-14	67.0
MS.gene008181.t1	AT3G58780	46.479	71	38	0	6	76	14	84	3.94e-14	67.0
MS.gene008181.t1	AT2G42830	47.059	68	36	0	9	76	17	84	4.10e-14	66.6
MS.gene008181.t1	AT3G58780	46.479	71	38	0	6	76	20	90	4.80e-14	66.6
MS.gene008181.t1	AT4G37940	45.588	68	37	0	9	76	2	69	5.21e-14	65.5
MS.gene008181.t1	AT4G37940	45.588	68	37	0	9	76	2	69	6.53e-14	65.9
MS.gene008181.t1	AT4G37940	45.588	68	37	0	9	76	2	69	7.32e-14	65.9
MS.gene008181.t1	AT3G58780	46.479	71	38	0	6	76	14	84	7.61e-14	66.6
MS.gene008181.t1	AT3G57230	45.588	68	37	0	9	76	2	69	1.06e-13	65.5
MS.gene008181.t1	AT3G57230	45.588	68	37	0	9	76	2	69	1.06e-13	65.5
MS.gene008181.t1	AT3G30260	42.029	69	39	1	9	76	2	70	1.53e-13	65.5
MS.gene008181.t1	AT4G11880	45.833	72	38	1	10	80	3	74	3.01e-13	63.9
MS.gene008181.t1	AT4G11880	45.833	72	38	1	10	80	3	74	3.01e-13	63.9
MS.gene008181.t1	AT4G11880	45.833	72	38	1	10	80	3	74	3.46e-13	63.2
MS.gene008181.t1	AT4G11880	45.833	72	38	1	10	80	3	74	3.46e-13	63.2
MS.gene008181.t1	AT5G60910	44.928	69	37	1	9	76	2	70	3.65e-13	64.3
MS.gene008181.t1	AT2G22540	40.385	104	52	2	10	108	3	101	3.90e-13	63.9
MS.gene008181.t1	AT5G10140	43.056	72	40	1	9	79	2	73	4.28e-13	62.8
MS.gene008181.t1	AT5G10140	43.056	72	40	1	9	79	2	73	4.44e-13	63.2
MS.gene008181.t1	AT2G22630	45.588	68	37	0	9	76	2	69	5.10e-13	63.5
MS.gene008181.t1	AT2G22630	45.588	68	37	0	9	76	2	69	5.10e-13	63.5
MS.gene008181.t1	AT5G10140	43.056	72	40	1	9	79	2	73	5.96e-13	62.8
MS.gene008181.t1	AT1G26310	44.928	69	37	1	9	76	2	70	6.18e-13	63.5
MS.gene008181.t1	AT1G28460	30.631	111	77	0	6	116	5	115	6.42e-13	62.8
MS.gene008181.t1	AT5G10140	43.056	72	40	1	9	79	2	73	6.63e-13	62.8
MS.gene008181.t1	AT1G17310	46.835	79	40	1	8	86	47	123	6.68e-13	63.2
MS.gene008181.t1	AT1G17310	46.835	79	40	1	8	86	58	134	8.64e-13	63.2
MS.gene008181.t1	AT1G69120	34.821	112	56	3	9	119	2	97	1.44e-12	62.4
MS.gene008181.t1	AT2G14210	44.928	69	37	1	9	76	2	70	1.58e-12	62.4
MS.gene008181.t1	AT2G14210	44.928	69	37	1	9	76	2	70	1.86e-12	62.0
MS.gene008181.t1	AT5G15800	46.377	69	36	1	9	76	2	70	2.13e-12	62.4
MS.gene008181.t1	AT1G69120	34.821	112	56	3	9	119	2	97	2.59e-12	62.0
MS.gene008181.t1	AT5G15800	46.377	69	36	1	9	76	2	70	3.30e-12	61.6
MS.gene008181.t1	AT3G02310	44.928	69	37	1	9	76	2	70	6.36e-12	60.1
MS.gene008181.t1	AT5G23260	43.662	71	38	1	9	77	2	72	8.37e-12	60.5
MS.gene008181.t1	AT5G23260	43.662	71	38	1	9	77	18	88	8.50e-12	60.8
MS.gene008181.t1	AT5G23260	43.662	71	38	1	9	77	18	88	8.54e-12	60.8
MS.gene008181.t1	AT5G23260	43.662	71	38	1	9	77	2	72	8.97e-12	60.5
MS.gene008181.t1	AT2G03710	50.000	56	28	0	9	64	2	57	9.49e-12	59.7
MS.gene008181.t1	AT3G54340	31.496	127	75	2	9	124	2	127	1.80e-11	59.3
MS.gene008181.t1	AT3G58780	45.070	71	32	1	6	76	14	77	1.86e-11	59.7
MS.gene008181.t1	AT1G24260	51.786	56	27	0	9	64	2	57	2.09e-11	59.3
MS.gene008181.t1	AT1G24260	44.928	69	37	1	9	76	2	70	2.09e-11	59.3
MS.gene008181.t1	AT2G03710	50.000	56	28	0	9	64	2	57	2.21e-11	59.3
MS.gene008181.t1	AT2G03710	50.000	56	28	0	9	64	2	57	2.31e-11	59.3
MS.gene008181.t1	AT1G24260	44.928	69	37	1	9	76	2	70	2.36e-11	59.3
MS.gene008181.t1	AT3G58780	47.541	61	32	0	6	66	14	74	2.66e-11	59.3
MS.gene008181.t1	AT1G29962	33.000	100	64	2	6	102	5	104	2.78e-11	58.2
MS.gene008181.t1	AT3G02310	44.928	69	37	1	9	76	2	70	3.30e-11	58.9
MS.gene008181.t1	AT5G65070	40.580	69	40	1	9	76	2	70	3.88e-11	57.0
MS.gene008181.t1	AT5G65050	39.130	69	41	1	9	76	2	70	4.89e-11	57.4
MS.gene008181.t1	AT5G65050	39.130	69	41	1	9	76	2	70	9.58e-11	57.0

Find 17 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGCAAGATGCCATTGTTT+TGG	0.345850	3.4:+68862513	None:intergenic
AGCGAGCTTTGCACCCTTTG+TGG	0.386397	3.4:-68862641	MS.gene008181:CDS
CATAAATCGTTATATCTCTC+AGG	0.481105	3.4:-68862543	MS.gene008181:CDS
AGCAACATATGCACCACAAA+GGG	0.521852	3.4:+68862628	None:intergenic
GCATCGTTCATATGAGTCAA+TGG	0.541468	3.4:+68862446	None:intergenic
AATGAGCCTCATTTCGCAGA+TGG	0.559491	3.4:+68862386	None:intergenic
TGGCCACACAAATGTAGAAA+CGG	0.561072	3.4:-68862567	MS.gene008181:CDS
TATATTCTCACCTGGTGAGA+AGG	0.562933	3.4:-68862600	MS.gene008181:CDS
TTTGAGCCATCTGCGAAATG+AGG	0.587155	3.4:-68862392	None:intergenic
TGACCGTTTCTACATTTGTG+TGG	0.593761	3.4:+68862564	None:intergenic
AATGAAAACACCTTCTCACC+AGG	0.598148	3.4:+68862590	None:intergenic
GCTCTGATTATATTCTCACC+TGG	0.608989	3.4:-68862608	MS.gene008181:CDS
CAGGTCCCACCCCAAAACAA+TGG	0.630259	3.4:-68862524	MS.gene008181:CDS
GAGCAACATATGCACCACAA+AGG	0.640810	3.4:+68862627	None:intergenic
TCGAGTCGAATGAAAACTCG+AGG	0.674187	3.4:-68862755	MS.gene008181:CDS
ATGGCTCAAACTCATTACAA+CGG	0.727817	3.4:+68862405	None:intergenic
TGGCATCTTGCAATTCATCG+AGG	0.757727	3.4:-68862504	MS.gene008181:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCTTTTTCATCTCAATCTTT+TGG	+	chr3.4:68862426-68862445	None:intergenic	25.0%
	CATAAATCGTTATATCTCTC+AGG	-	chr3.4:68862609-68862628	MS.gene008181:CDS	30.0%
	ATGGCTCAAACTCATTACAA+CGG	+	chr3.4:68862750-68862769	None:intergenic	35.0%
!	ATTGCAAGATGCCATTGTTT+TGG	+	chr3.4:68862642-68862661	None:intergenic	35.0%
!	TTGCAAGATGCCATTGTTTT+GGG	+	chr3.4:68862641-68862660	None:intergenic	35.0%
	AATGAAAACACCTTCTCACC+AGG	+	chr3.4:68862565-68862584	None:intergenic	40.0%
	AGCAACATATGCACCACAAA+GGG	+	chr3.4:68862527-68862546	None:intergenic	40.0%
	GCATCGTTCATATGAGTCAA+TGG	+	chr3.4:68862709-68862728	None:intergenic	40.0%
	GCTCTGATTATATTCTCACC+TGG	-	chr3.4:68862544-68862563	MS.gene008181:CDS	40.0%
	TATATTCTCACCTGGTGAGA+AGG	-	chr3.4:68862552-68862571	MS.gene008181:CDS	40.0%
	TGACCGTTTCTACATTTGTG+TGG	+	chr3.4:68862591-68862610	None:intergenic	40.0%
	TGGCCACACAAATGTAGAAA+CGG	-	chr3.4:68862585-68862604	MS.gene008181:CDS	40.0%
!!	CTATTTTGAAGAGCCCACTA+TGG	+	chr3.4:68862489-68862508	None:intergenic	40.0%
!!	TGCAAGATGCCATTGTTTTG+GGG	+	chr3.4:68862640-68862659	None:intergenic	40.0%
!!!	ACTTTTTCGAAGCTCCATAG+TGG	-	chr3.4:68862472-68862491	MS.gene008181:CDS	40.0%
!!!	CTTTTTCGAAGCTCCATAGT+GGG	-	chr3.4:68862473-68862492	MS.gene008181:CDS	40.0%
!!!	GGTGAGAAGGTGTTTTCATT+TGG	-	chr3.4:68862565-68862584	MS.gene008181:CDS	40.0%
	GAGCAACATATGCACCACAA+AGG	+	chr3.4:68862528-68862547	None:intergenic	45.0%
	GCTTCGAAAAAGTCACTTGC+AGG	+	chr3.4:68862465-68862484	None:intergenic	45.0%
	TCGAGTCGAATGAAAACTCG+AGG	-	chr3.4:68862397-68862416	MS.gene008181:CDS	45.0%
	TGGCATCTTGCAATTCATCG+AGG	-	chr3.4:68862648-68862667	MS.gene008181:CDS	45.0%
!!	GCTTGCTCACTTTTGCGTTA+CGG	+	chr3.4:68862678-68862697	None:intergenic	45.0%
!!!	AAGATGCCATTGTTTTGGGG+TGG	+	chr3.4:68862637-68862656	None:intergenic	45.0%
!!!	AGATGCCATTGTTTTGGGGT+GGG	+	chr3.4:68862636-68862655	None:intergenic	45.0%
	AGCGAGCTTTGCACCCTTTG+TGG	-	chr3.4:68862511-68862530	MS.gene008181:CDS	55.0%
	CAGGTCCCACCCCAAAACAA+TGG	-	chr3.4:68862628-68862647	MS.gene008181:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	68862394	68862780	68862394	ID=MS.gene008181
chr3.4	mRNA	68862394	68862780	68862394	ID=MS.gene008181.t1;Parent=MS.gene008181
chr3.4	exon	68862394	68862780	68862394	ID=MS.gene008181.t1.exon1;Parent=MS.gene008181.t1
chr3.4	CDS	68862394	68862780	68862394	ID=cds.MS.gene008181.t1;Parent=MS.gene008181.t1

Gene Sequence

>MS.gene008181

ATGTCGAGTCGAATGAAAACTCGAGGTCGCCAAAAGATTGAGATGAAAAAGATGAGAAATGAAAGCAACCTGCAAGTGACTTTTTCGAAGCTCCATAGTGGGCTCTTCAAAATAGCTAGCGAGCTTTGCACCCTTTGTGGTGCATATGTTGCTCTGATTATATTCTCACCTGGTGAGAAGGTGTTTTCATTTGGCCACACAAATGTAGAAACGGTCATAAATCGTTATATCTCTCAGGTCCCACCCCAAAACAATGGCATCTTGCAATTCATCGAGGCATACCGTAACGCAAAAGTGAGCAAGCTCAATGCTCCATTGACTCATATGAACGATGCACTAGACATCGAGAAGAACCGTTGTAATGAGTTTGAGCCATCTGCGAAATGA

Protein sequence

>MS.gene008181.t1

MSSRMKTRGRQKIEMKKMRNESNLQVTFSKLHSGLFKIASELCTLCGAYVALIIFSPGEKVFSFGHTNVETVINRYISQVPPQNNGILQFIEAYRNAKVSKLNAPLTHMNDALDIEKNRCNEFEPSAK