Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008326.t1 | RHN68670.1 | 78.6 | 168 | 26 | 2 | 1 | 168 | 55 | 212 | 2.60E-66 | 261.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008326.t1 | Q6K5K2 | 34.9 | 109 | 68 | 2 | 33 | 141 | 377 | 482 | 3.5e-11 | 69.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008326.t1 | A0A396ITE5 | 78.6 | 168 | 26 | 2 | 1 | 168 | 55 | 212 | 1.9e-66 | 261.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene008326.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008326.t1 | MTR_3g075140 | 77.966 | 118 | 16 | 2 | 48 | 165 | 61 | 168 | 3.38e-47 | 154 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAACTTCTACACACTATT+TGG | 0.203177 | 3.1:-54178442 | None:intergenic |
| TCAACGAAATTTCAAAGTTT+TGG | 0.243919 | 3.1:+54177758 | MS.gene008326:CDS |
| TCGGCTCACAAGCTTGAAAT+TGG | 0.273569 | 3.1:+54178244 | MS.gene008326:CDS |
| CAACTCTTTCTACAGCTTCA+TGG | 0.328763 | 3.1:+54177731 | MS.gene008326:CDS |
| ACTTGATGTGCCAATGCATA+TGG | 0.375752 | 3.1:+54178399 | MS.gene008326:CDS |
| TGTTACAGCCCATCTCTTAC+CGG | 0.383658 | 3.1:-54178167 | None:intergenic |
| ATGAAGCTGTAGAAAGAGTT+GGG | 0.417195 | 3.1:-54177729 | None:intergenic |
| AGCCCATCTCTTACCGGTTG+AGG | 0.418637 | 3.1:-54178161 | None:intergenic |
| CTCGAGGATTCAACCTCAAC+CGG | 0.431548 | 3.1:+54178148 | MS.gene008326:CDS |
| AAATCCTACCATGTCATTGT+TGG | 0.435100 | 3.1:+54178108 | MS.gene008326:CDS |
| CTTGATGTGCCAATGCATAT+GGG | 0.492897 | 3.1:+54178400 | MS.gene008326:CDS |
| CATGAAGCTGTAGAAAGAGT+TGG | 0.523427 | 3.1:-54177730 | None:intergenic |
| CAACCTCAACCGGTAAGAGA+TGG | 0.526023 | 3.1:+54178158 | MS.gene008326:CDS |
| ATGGTAGGATTTAACTTAGG+AGG | 0.553387 | 3.1:-54178097 | None:intergenic |
| GTTCGCAATCAACAAGTACA+AGG | 0.556424 | 3.1:+54177642 | MS.gene008326:CDS |
| AACCTCAACCGGTAAGAGAT+GGG | 0.557870 | 3.1:+54178159 | MS.gene008326:CDS |
| CCTACCATGTCATTGTTGGT+TGG | 0.564330 | 3.1:+54178112 | MS.gene008326:CDS |
| GTAACAGTATCAAAAGTTGA+TGG | 0.568143 | 3.1:+54178184 | MS.gene008326:CDS |
| GACATGGTAGGATTTAACTT+AGG | 0.591746 | 3.1:-54178100 | None:intergenic |
| TTGATGTGCCAATGCATATG+GGG | 0.595030 | 3.1:+54178401 | MS.gene008326:CDS |
| CCAACCAACAATGACATGGT+AGG | 0.598154 | 3.1:-54178112 | None:intergenic |
| TTGATGTCAAGATCTATGAC+AGG | 0.601226 | 3.1:+54178305 | MS.gene008326:CDS |
| CCACCCTTCCTTAAAAGCAA+AGG | 0.608557 | 3.1:-54178209 | None:intergenic |
| CTTACCAACCAACAATGACA+TGG | 0.609549 | 3.1:-54178116 | None:intergenic |
| TGAAGCTGTAGAAAGAGTTG+GGG | 0.621954 | 3.1:-54177728 | None:intergenic |
| AAGAGAAGTAGGTGCAGCGG+TGG | 0.623835 | 3.1:+54178373 | MS.gene008326:CDS |
| TGGTAAGAAAATCTATCTCG+AGG | 0.647585 | 3.1:+54178132 | MS.gene008326:CDS |
| GAAGCTGTAGAAAGAGTTGG+GGG | 0.707148 | 3.1:-54177727 | None:intergenic |
| TTCGCAATCAACAAGTACAA+GGG | 0.707970 | 3.1:+54177643 | MS.gene008326:CDS |
| AGTGATCAAAGAATCTACAG+TGG | 0.718405 | 3.1:-54177683 | None:intergenic |
| TGATGTGCCAATGCATATGG+GGG | 0.729130 | 3.1:+54178402 | MS.gene008326:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTCTGAAAAAAGAAAT+AGG | + | chr3.1:54178347-54178366 | MS.gene008326:CDS | 15.0% |
| !! | AAAAGGAAAGTAAAATATTG+AGG | - | chr3.1:54177798-54177817 | None:intergenic | 20.0% |
| !!! | ATTTTATTTCAGCAATGAAA+AGG | - | chr3.1:54177815-54177834 | None:intergenic | 20.0% |
| ! | ATCCTGCAGTTTAAAAAAAA+TGG | - | chr3.1:54178074-54178093 | None:intergenic | 25.0% |
| !! | TCAACGAAATTTCAAAGTTT+TGG | + | chr3.1:54177758-54177777 | MS.gene008326:CDS | 25.0% |
| !!! | TACCATTTTTTTTAAACTGC+AGG | + | chr3.1:54178069-54178088 | MS.gene008326:intron | 25.0% |
| ATGGTTTGATTATGTTGTTG+TGG | + | chr3.1:54177924-54177943 | MS.gene008326:intron | 30.0% | |
| GAAATAGGAAAAAGAGAAGT+AGG | + | chr3.1:54178362-54178381 | MS.gene008326:CDS | 30.0% | |
| GTAACAGTATCAAAAGTTGA+TGG | + | chr3.1:54178184-54178203 | MS.gene008326:CDS | 30.0% | |
| ! | AATCAGTGATTTTACTTTGC+AGG | - | chr3.1:54177871-54177890 | None:intergenic | 30.0% |
| AAATCCTACCATGTCATTGT+TGG | + | chr3.1:54178108-54178127 | MS.gene008326:CDS | 35.0% | |
| ACATGCAGCATCATAATTCA+TGG | - | chr3.1:54178046-54178065 | None:intergenic | 35.0% | |
| AGTGATCAAAGAATCTACAG+TGG | - | chr3.1:54177686-54177705 | None:intergenic | 35.0% | |
| ATCAAACCATGTGAACAGTT+TGG | - | chr3.1:54177914-54177933 | None:intergenic | 35.0% | |
| ATGAAGCTGTAGAAAGAGTT+GGG | - | chr3.1:54177732-54177751 | None:intergenic | 35.0% | |
| ATGTGAACAGTTTGGTATCA+AGG | - | chr3.1:54177906-54177925 | None:intergenic | 35.0% | |
| TGGTAAGAAAATCTATCTCG+AGG | + | chr3.1:54178132-54178151 | MS.gene008326:CDS | 35.0% | |
| TTCGCAATCAACAAGTACAA+GGG | + | chr3.1:54177643-54177662 | MS.gene008326:CDS | 35.0% | |
| TTGATACCAAACTGTTCACA+TGG | + | chr3.1:54177905-54177924 | MS.gene008326:intron | 35.0% | |
| TTGATGTCAAGATCTATGAC+AGG | + | chr3.1:54178305-54178324 | MS.gene008326:CDS | 35.0% | |
| ! | ATGGTAGGATTTAACTTAGG+AGG | - | chr3.1:54178100-54178119 | None:intergenic | 35.0% |
| ! | GACATGGTAGGATTTAACTT+AGG | - | chr3.1:54178103-54178122 | None:intergenic | 35.0% |
| !! | GTTCCTTTGCTTTTAAGGAA+GGG | + | chr3.1:54178206-54178225 | MS.gene008326:CDS | 35.0% |
| !! | TGATGGTTCCTTTGCTTTTA+AGG | + | chr3.1:54178201-54178220 | MS.gene008326:CDS | 35.0% |
| AAAAAGAGAAGTAGGTGCAG+CGG | + | chr3.1:54178370-54178389 | MS.gene008326:CDS | 40.0% | |
| ACTTGATGTGCCAATGCATA+TGG | + | chr3.1:54178399-54178418 | MS.gene008326:CDS | 40.0% | |
| CAACTCTTTCTACAGCTTCA+TGG | + | chr3.1:54177731-54177750 | MS.gene008326:CDS | 40.0% | |
| CATGAAGCTGTAGAAAGAGT+TGG | - | chr3.1:54177733-54177752 | None:intergenic | 40.0% | |
| CTTACCAACCAACAATGACA+TGG | - | chr3.1:54178119-54178138 | None:intergenic | 40.0% | |
| CTTGATGTGCCAATGCATAT+GGG | + | chr3.1:54178400-54178419 | MS.gene008326:CDS | 40.0% | |
| GAACAGTTTGGTATCAAGGT+AGG | - | chr3.1:54177902-54177921 | None:intergenic | 40.0% | |
| GTTCGCAATCAACAAGTACA+AGG | + | chr3.1:54177642-54177661 | MS.gene008326:CDS | 40.0% | |
| TGAAGCTGTAGAAAGAGTTG+GGG | - | chr3.1:54177731-54177750 | None:intergenic | 40.0% | |
| TTGATGTGCCAATGCATATG+GGG | + | chr3.1:54178401-54178420 | MS.gene008326:CDS | 40.0% | |
| !! | CTTTGCTTTTAAGGAAGGGT+GGG | + | chr3.1:54178210-54178229 | MS.gene008326:CDS | 40.0% |
| !! | GGTTCCTTTGCTTTTAAGGA+AGG | + | chr3.1:54178205-54178224 | MS.gene008326:CDS | 40.0% |
| AACCTCAACCGGTAAGAGAT+GGG | + | chr3.1:54178159-54178178 | MS.gene008326:CDS | 45.0% | |
| ACATCAACCCCCATATGCAT+TGG | - | chr3.1:54178412-54178431 | None:intergenic | 45.0% | |
| CCAACCAACAATGACATGGT+AGG | - | chr3.1:54178115-54178134 | None:intergenic | 45.0% | |
| CCACCCTTCCTTAAAAGCAA+AGG | - | chr3.1:54178212-54178231 | None:intergenic | 45.0% | |
| CCTACCATGTCATTGTTGGT+TGG | + | chr3.1:54178112-54178131 | MS.gene008326:CDS | 45.0% | |
| GAAGCTGTAGAAAGAGTTGG+GGG | - | chr3.1:54177730-54177749 | None:intergenic | 45.0% | |
| TCGGCTCACAAGCTTGAAAT+TGG | + | chr3.1:54178244-54178263 | MS.gene008326:CDS | 45.0% | |
| TGATGTGCCAATGCATATGG+GGG | + | chr3.1:54178402-54178421 | MS.gene008326:CDS | 45.0% | |
| TGTTACAGCCCATCTCTTAC+CGG | - | chr3.1:54178170-54178189 | None:intergenic | 45.0% | |
| ! | AGGGTGGGATGTTTTCTCAT+CGG | + | chr3.1:54178225-54178244 | MS.gene008326:CDS | 45.0% |
| !! | ATGCATATGGGGGTTGATGT+AGG | + | chr3.1:54178412-54178431 | MS.gene008326:CDS | 45.0% |
| !! | CCTTTGCTTTTAAGGAAGGG+TGG | + | chr3.1:54178209-54178228 | MS.gene008326:CDS | 45.0% |
| CAACCTCAACCGGTAAGAGA+TGG | + | chr3.1:54178158-54178177 | MS.gene008326:CDS | 50.0% | |
| CTCGAGGATTCAACCTCAAC+CGG | + | chr3.1:54178148-54178167 | MS.gene008326:CDS | 50.0% | |
| !! | CATATGGGGGTTGATGTAGG+AGG | + | chr3.1:54178415-54178434 | MS.gene008326:CDS | 50.0% |
| AAGAGAAGTAGGTGCAGCGG+TGG | + | chr3.1:54178373-54178392 | MS.gene008326:CDS | 55.0% | |
| GCTGTAGAAAGAGTTGGGGG+AGG | - | chr3.1:54177727-54177746 | None:intergenic | 55.0% | |
| ! | AGCCCATCTCTTACCGGTTG+AGG | - | chr3.1:54178164-54178183 | None:intergenic | 55.0% |
| !! | GAGTTGGGGGAGGTTGATGT+TGG | - | chr3.1:54177717-54177736 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 54177639 | 54178456 | 54177639 | ID=MS.gene008326 |
| chr3.1 | mRNA | 54177639 | 54178456 | 54177639 | ID=MS.gene008326.t1;Parent=MS.gene008326 |
| chr3.1 | exon | 54177639 | 54177779 | 54177639 | ID=MS.gene008326.t1.exon1;Parent=MS.gene008326.t1 |
| chr3.1 | CDS | 54177639 | 54177779 | 54177639 | ID=cds.MS.gene008326.t1;Parent=MS.gene008326.t1 |
| chr3.1 | exon | 54178091 | 54178456 | 54178091 | ID=MS.gene008326.t1.exon2;Parent=MS.gene008326.t1 |
| chr3.1 | CDS | 54178091 | 54178456 | 54178091 | ID=cds.MS.gene008326.t1;Parent=MS.gene008326.t1 |
| Gene Sequence |
| Protein sequence |
