AlfalfaGEDB | MS.gene008483

M. sativa cultivar XinJiangDaYe / MS.gene008483

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008483.t1	PNX98523.1	69.6	161	49	0	1	161	1	161	2.90E-54	221.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008483.t1	Q9FJK3	42.9	154	88	0	1	154	1	154	6.8e-28	125.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008483.t1	A0A2K3N650	69.6	161	49	0	1	161	1	161	2.1e-54	221.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene008483.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008483.t1	MTR_2g035580	66.456	158	53	0	1	158	1	158	6.60e-63	191
MS.gene008483.t1	MTR_3g467080	51.553	161	77	1	1	160	1	161	7.14e-50	157
MS.gene008483.t1	MTR_3g466980	45.625	160	87	0	1	160	1	160	3.94e-46	150
MS.gene008483.t1	MTR_4g063790	46.626	163	86	1	1	162	1	163	3.72e-45	146
MS.gene008483.t1	MTR_2g016210	45.181	166	84	3	1	161	1	164	7.10e-39	132
MS.gene008483.t1	MTR_3g031100	44.785	163	89	1	1	162	1	163	2.29e-38	131
MS.gene008483.t1	MTR_5g075380	35.948	153	98	0	1	153	1	153	3.00e-36	123
MS.gene008483.t1	MTR_3g465410	46.269	134	71	1	29	161	1	134	8.05e-35	119
MS.gene008483.t1	MTR_3g065100	42.857	154	88	0	1	154	1	154	1.69e-34	121
MS.gene008483.t1	MTR_4g032620	35.714	154	98	1	1	153	1	154	1.01e-31	114
MS.gene008483.t1	MTR_3g031240	39.264	163	96	2	1	160	1	163	1.54e-30	109
MS.gene008483.t1	MTR_4g031910	40.645	155	91	1	1	154	1	155	2.42e-30	110
MS.gene008483.t1	MTR_3g466830	37.671	146	91	0	7	152	6	151	4.82e-29	105
MS.gene008483.t1	MTR_8g036130	38.217	157	94	2	1	154	1	157	4.91e-28	104
MS.gene008483.t1	MTR_1g090697	34.615	156	101	1	1	156	1	155	5.58e-27	99.4
MS.gene008483.t1	MTR_5g047580	35.256	156	100	1	1	156	1	155	6.85e-27	99.4
MS.gene008483.t1	MTR_1g077390	34.641	153	99	1	1	153	1	152	3.89e-25	94.7
MS.gene008483.t1	MTR_4g028720	37.908	153	94	1	1	153	1	152	6.03e-25	94.4
MS.gene008483.t1	MTR_4g032260	37.419	155	96	1	1	154	1	155	4.39e-24	92.0
MS.gene008483.t1	MTR_1g090783	32.895	152	101	1	7	158	6	156	5.97e-24	91.7
MS.gene008483.t1	MTR_2g035610	38.655	119	73	0	1	119	1	119	3.12e-23	89.0
MS.gene008483.t1	MTR_7g011950	35.948	153	97	1	1	153	1	152	5.78e-23	89.4
MS.gene008483.t1	MTR_1g077320	33.987	153	100	1	1	153	1	152	6.10e-23	89.4
MS.gene008483.t1	MTR_4g032290	37.580	157	97	1	7	162	7	163	7.16e-23	90.9
MS.gene008483.t1	MTR_1g084950	37.013	154	96	1	1	154	1	153	1.93e-22	87.8
MS.gene008483.t1	MTR_1g090710	33.333	156	100	3	1	154	1	154	3.97e-22	87.0
MS.gene008483.t1	MTR_3g466890	31.469	143	98	0	7	149	6	148	6.86e-20	81.3
MS.gene008483.t1	MTR_3g466930	35.156	128	71	3	32	153	2	123	5.93e-15	67.8
MS.gene008483.t1	MTR_1g077300	33.125	160	104	2	1	160	1	157	9.11e-15	67.8
MS.gene008483.t1	MTR_3g466900	30.328	122	85	0	32	153	2	123	1.17e-14	67.0
MS.gene008483.t1	MTR_4g028800	37.143	70	42	1	1	70	1	68	4.25e-13	61.2
MS.gene008483.t1	MTR_5g047560	28.873	142	84	2	1	142	1	125	5.05e-13	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008483.t1	AT5G48670	43.226	155	86	2	1	154	1	154	1.99e-31	115
MS.gene008483.t1	AT1G65330	40.385	156	91	2	7	161	6	160	1.46e-30	112
MS.gene008483.t1	AT3G05860	36.913	149	94	0	1	149	1	149	7.00e-29	105
MS.gene008483.t1	AT3G05860	36.913	149	94	0	1	149	1	149	2.17e-28	105
MS.gene008483.t1	AT3G05860	36.913	149	94	0	1	149	1	149	2.41e-28	105
MS.gene008483.t1	AT1G65300	35.948	153	98	0	4	156	3	155	2.92e-25	98.2
MS.gene008483.t1	AT1G31640	35.948	153	93	2	6	156	5	154	2.87e-22	92.4
MS.gene008483.t1	AT5G27810	43.820	89	50	0	29	117	1	89	5.56e-22	85.5
MS.gene008483.t1	AT5G27960	31.613	155	106	0	7	161	5	159	8.36e-19	81.6
MS.gene008483.t1	AT5G26650	31.613	155	106	0	7	161	5	159	1.21e-18	81.6
MS.gene008483.t1	AT1G31630	33.094	139	86	2	7	142	6	140	1.48e-18	81.3
MS.gene008483.t1	AT2G28700	34.951	103	67	0	1	103	1	103	4.25e-18	79.7
MS.gene008483.t1	AT1G22590	29.878	164	100	3	1	158	1	155	6.49e-18	76.3
MS.gene008483.t1	AT5G26630	29.814	161	113	0	1	161	1	161	5.24e-17	75.1
MS.gene008483.t1	AT5G06500	30.000	130	83	2	1	126	1	126	2.20e-15	71.2
MS.gene008483.t1	AT2G40210	28.058	139	99	1	1	139	1	138	4.71e-13	65.9
MS.gene008483.t1	AT5G26580	25.478	157	103	1	1	157	1	143	2.74e-11	60.8

Find 29 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCATTCATTTCATTCTTCT+TGG	0.187611	3.3:+55609175	None:intergenic
CTCCATTTCAATCTTCATCT+TGG	0.285395	3.3:+55609024	None:intergenic
GAAAGGCAACATTTGGAAAA+AGG	0.291917	3.3:-55609657	MS.gene008483:CDS
TTTGTTTCTTTAACTTCTCT+AGG	0.340953	3.3:+55609207	None:intergenic
ATTCATTCATACGGGTGAAT+TGG	0.359950	3.3:-55609141	MS.gene008483:CDS
TGCTTATTGTTGAGAACCTT+AGG	0.364399	3.3:-55609079	MS.gene008483:CDS
TCCATTTCAATCTTCATCTT+GGG	0.367020	3.3:+55609025	None:intergenic
TCGAAGAGAAAGGCAACATT+TGG	0.402575	3.3:-55609664	MS.gene008483:CDS
TGAACTTGTCCAACACCATA+TGG	0.431567	3.3:+55609306	None:intergenic
TTGTTTCTTTAACTTCTCTA+GGG	0.453550	3.3:+55609208	None:intergenic
AATTTGTCAATTCATTCATA+CGG	0.461709	3.3:-55609150	MS.gene008483:CDS
AATGAAATCACCACCTTATG+TGG	0.471722	3.3:-55609401	MS.gene008483:CDS
ACATACATCAATTCCACATA+AGG	0.491397	3.3:+55609388	None:intergenic
TTCTTTGTTCGACTTCCCTA+AGG	0.503187	3.3:+55609063	None:intergenic
ATTTGTCAATTCATTCATAC+GGG	0.508251	3.3:-55609149	MS.gene008483:CDS
ATTCATACGGGTGAATTGGA+TGG	0.523018	3.3:-55609137	MS.gene008483:CDS
GCTTATTGTTGAGAACCTTA+GGG	0.536620	3.3:-55609078	MS.gene008483:CDS
AACTGATGACTCGAAGAGAA+AGG	0.544142	3.3:-55609674	MS.gene008483:CDS
AAAATCGATGGTGGATCATG+AGG	0.544806	3.3:-55609258	MS.gene008483:CDS
GGTGAATTGGATGGAAATGT+TGG	0.586534	3.3:-55609128	MS.gene008483:CDS
AAGTCGAACAAAGAATCGAA+TGG	0.606367	3.3:-55609055	MS.gene008483:CDS
AAATCGATGGTGGATCATGA+GGG	0.613825	3.3:-55609257	MS.gene008483:CDS
TGTATGTGGTATAATCTATG+AGG	0.618109	3.3:-55609372	MS.gene008483:CDS
TACATCAATTCCACATAAGG+TGG	0.624843	3.3:+55609391	None:intergenic
GGAAAACAACACTCAAGCAA+TGG	0.638025	3.3:-55609351	MS.gene008483:CDS
ATATGAACGAAGAAAATCGA+TGG	0.638659	3.3:-55609270	MS.gene008483:CDS
TGAACGAAGAAAATCGATGG+TGG	0.640332	3.3:-55609267	MS.gene008483:CDS
TTATGTGGAATTGATGTATG+TGG	0.644295	3.3:-55609386	MS.gene008483:CDS
TGGATCATGAGGGCTTCTTG+AGG	0.670340	3.3:-55609247	MS.gene008483:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTGCTTCATAAAAATTA+AGG	+	chr3.3:55609131-55609150	None:intergenic	15.0%
!!	AATTTGTCAATTCATTCATA+CGG	-	chr3.3:55609587-55609606	MS.gene008483:intron	20.0%
!!	ACTTTATACTATAAATGACA+TGG	+	chr3.3:55609253-55609272	None:intergenic	20.0%
!!	TGATGTTCATAAATTTCTAA+TGG	+	chr3.3:55609171-55609190	None:intergenic	20.0%
!	ATCATTCATTTCATTCTTCT+TGG	+	chr3.3:55609565-55609584	None:intergenic	25.0%
!	ATTTGTCAATTCATTCATAC+GGG	-	chr3.3:55609588-55609607	MS.gene008483:intron	25.0%
!!	TCAATTTTACATTTCTCTGA+CGG	-	chr3.3:55609274-55609293	MS.gene008483:CDS	25.0%
!!	TTGTTTCTTTAACTTCTCTA+GGG	+	chr3.3:55609532-55609551	None:intergenic	25.0%
!!	TTTGTTTCTTTAACTTCTCT+AGG	+	chr3.3:55609533-55609552	None:intergenic	25.0%
	ACATACATCAATTCCACATA+AGG	+	chr3.3:55609352-55609371	None:intergenic	30.0%
	ACATTTGGAAAAAGGAAACA+AGG	-	chr3.3:55609088-55609107	MS.gene008483:CDS	30.0%
	ATATGAACGAAGAAAATCGA+TGG	-	chr3.3:55609467-55609486	MS.gene008483:intron	30.0%
	ATCCAAAGAAAAAGTCCATA+TGG	-	chr3.3:55609416-55609435	MS.gene008483:CDS	30.0%
	TGTATGTGGTATAATCTATG+AGG	-	chr3.3:55609365-55609384	MS.gene008483:CDS	30.0%
	TTATGTGGAATTGATGTATG+TGG	-	chr3.3:55609351-55609370	MS.gene008483:CDS	30.0%
!	GATTTTCTTCGTTCATATTC+AGG	+	chr3.3:55609465-55609484	None:intergenic	30.0%
!!	ATATGGACTTTTTCTTTGGA+TGG	+	chr3.3:55609417-55609436	None:intergenic	30.0%
!!	CTCTTGCTATATTTTCAAAC+AGG	-	chr3.3:55609297-55609316	MS.gene008483:CDS	30.0%
	AAGTCGAACAAAGAATCGAA+TGG	-	chr3.3:55609682-55609701	MS.gene008483:CDS	35.0%
	AATGAAATCACCACCTTATG+TGG	-	chr3.3:55609336-55609355	MS.gene008483:CDS	35.0%
	AGAAAAAGTCCATATGGTGT+TGG	-	chr3.3:55609422-55609441	MS.gene008483:intron	35.0%
	ATTCATTCATACGGGTGAAT+TGG	-	chr3.3:55609596-55609615	MS.gene008483:intron	35.0%
	GAAAGGCAACATTTGGAAAA+AGG	-	chr3.3:55609080-55609099	MS.gene008483:CDS	35.0%
	GCTTATTGTTGAGAACCTTA+GGG	-	chr3.3:55609659-55609678	MS.gene008483:CDS	35.0%
	TACATCAATTCCACATAAGG+TGG	+	chr3.3:55609349-55609368	None:intergenic	35.0%
	TGCTTATTGTTGAGAACCTT+AGG	-	chr3.3:55609658-55609677	MS.gene008483:CDS	35.0%
!!	CACCATATGGACTTTTTCTT+TGG	+	chr3.3:55609421-55609440	None:intergenic	35.0%
	AACTGATGACTCGAAGAGAA+AGG	-	chr3.3:55609063-55609082	MS.gene008483:CDS	40.0%
	ATTCATACGGGTGAATTGGA+TGG	-	chr3.3:55609600-55609619	MS.gene008483:intron	40.0%
	GGAAAACAACACTCAAGCAA+TGG	-	chr3.3:55609386-55609405	MS.gene008483:CDS	40.0%
	GGTGAATTGGATGGAAATGT+TGG	-	chr3.3:55609609-55609628	MS.gene008483:intron	40.0%
	TCGAAGAGAAAGGCAACATT+TGG	-	chr3.3:55609073-55609092	MS.gene008483:CDS	40.0%
	TGAACGAAGAAAATCGATGG+TGG	-	chr3.3:55609470-55609489	MS.gene008483:intron	40.0%
	TGAACTTGTCCAACACCATA+TGG	+	chr3.3:55609434-55609453	None:intergenic	40.0%
	TTCTTTGTTCGACTTCCCTA+AGG	+	chr3.3:55609677-55609696	None:intergenic	40.0%
!!	AAAATCGATGGTGGATCATG+AGG	-	chr3.3:55609479-55609498	MS.gene008483:intron	40.0%
!!	AAATCGATGGTGGATCATGA+GGG	-	chr3.3:55609480-55609499	MS.gene008483:intron	40.0%
!!!	CTTCTCTAGGGCTTTTGCTA+TGG	+	chr3.3:55609520-55609539	None:intergenic	45.0%
	TGGATCATGAGGGCTTCTTG+AGG	-	chr3.3:55609490-55609509	MS.gene008483:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	55609031	55609728	55609031	ID=MS.gene008483
chr3.3	mRNA	55609031	55609728	55609031	ID=MS.gene008483.t1;Parent=MS.gene008483
chr3.3	exon	55609650	55609728	55609650	ID=MS.gene008483.t1.exon1;Parent=MS.gene008483.t1
chr3.3	CDS	55609650	55609728	55609650	ID=cds.MS.gene008483.t1;Parent=MS.gene008483.t1
chr3.3	exon	55609031	55609440	55609031	ID=MS.gene008483.t1.exon2;Parent=MS.gene008483.t1
chr3.3	CDS	55609031	55609440	55609031	ID=cds.MS.gene008483.t1;Parent=MS.gene008483.t1

Gene Sequence

>MS.gene008483

ATGGCTGCTAGAAATTCGAAGATTGAATACATAACTGATGACTCGAAGAGAAAGGCAACATTTGGAAAAAGGAAACAAGGTAATATACTAGCTTTCTCCTTAATTTTTATGAAGCAAATTCATATCAAACAAATTTACCATTAGAAATTTATGAACATCAGTATGAATCGTTTGATATTATGATTTATTTATTTTATCTTTTCAGACAATAATTTAAAGCCATGTCATTTATAGTATAAAGTATCAATTTTACATTTCTCTGACGGCTCTTGCTATATTTTCAAACAGGCATAATTAAGAAGATTAATGAAATCACCACCTTATGTGGAATTGATGTATGTGGTATAATCTATGAGGAAAACAACACTCAAGCAATGGTTTGTCCATCCAAAGAAAAAGTCCATATGGTGTTGGACAAGTTCATGAGTTTTCCTGAATATGAACGAAGAAAATCGATGGTGGATCATGAGGGCTTCTTGAGGCAATCCATAGCAAAAGCCCTAGAGAAGTTAAAGAAACAAAAGAACAACTCCAAGAAGAATGAAATGAATGATCTAATTTGTCAATTCATTCATACGGGTGAATTGGATGGAAATGTTGGTGAGAGTGATCTTGAAGATCTGTTGTTGCTTATTGTTGAGAACCTTAGGGAAGTCGAACAAAGAATCGAATGGATTCAAGCCCAAGATGAAGATTGA

Protein sequence

>MS.gene008483.t1

MAARNSKIEYITDDSKRKATFGKRKQGIIKKINEITTLCGIDVCGIIYEENNTQAMVCPSKEKVHMVLDKFMSFPEYERRKSMVDHEGFLRQSIAKALEKLKKQKNNSKKNEMNDLICQFIHTGELDGNVGESDLEDLLLLIVENLREVEQRIEWIQAQDED