AlfalfaGEDB | MS.gene008484

M. sativa cultivar XinJiangDaYe / MS.gene008484

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008484.t1	KEH34477.1	88.7	133	15	0	1	133	29	161	3.00E-57	231.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008484.t1	Q9FJK3	45.7	127	68	1	1	127	29	154	1.1e-23	110.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008484.t1	A0A072UY35	88.7	133	15	0	1	133	29	161	2.2e-57	231.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene008484.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008484.t1	MTR_3g467080	88.722	133	15	0	1	133	29	161	5.49e-84	243
MS.gene008484.t1	MTR_4g063790	78.195	133	29	0	1	133	29	161	3.19e-71	211
MS.gene008484.t1	MTR_3g465410	77.444	133	30	0	1	133	1	133	1.81e-70	208
MS.gene008484.t1	MTR_3g466980	52.756	127	57	2	1	126	29	153	1.28e-40	135
MS.gene008484.t1	MTR_3g031100	45.455	132	72	0	1	132	29	160	1.28e-34	120
MS.gene008484.t1	MTR_3g065100	47.154	123	64	1	4	126	32	153	1.35e-34	120
MS.gene008484.t1	MTR_3g031240	45.736	129	70	0	1	129	29	157	5.16e-32	112
MS.gene008484.t1	MTR_4g032290	43.077	130	74	0	4	133	32	161	2.74e-31	111
MS.gene008484.t1	MTR_2g016210	41.667	132	77	0	1	132	29	160	4.51e-30	108
MS.gene008484.t1	MTR_4g032260	39.837	123	74	0	6	128	34	156	6.32e-30	106
MS.gene008484.t1	MTR_2g035580	47.200	125	65	1	2	126	30	153	6.97e-30	105
MS.gene008484.t1	MTR_4g031910	42.400	125	72	0	4	128	32	156	2.29e-29	106
MS.gene008484.t1	MTR_4g032620	38.095	126	78	0	1	126	29	154	1.60e-28	104
MS.gene008484.t1	MTR_2g035610	49.451	91	46	0	4	94	32	122	2.23e-27	98.6
MS.gene008484.t1	MTR_5g075380	37.302	126	78	1	1	126	29	153	8.17e-27	98.2
MS.gene008484.t1	MTR_8g036130	39.695	131	77	1	1	129	29	159	2.73e-25	96.3
MS.gene008484.t1	MTR_3g466830	40.000	125	74	1	1	125	28	151	3.83e-24	91.3
MS.gene008484.t1	MTR_3g466900	34.921	126	81	1	4	129	2	126	1.00e-20	81.6
MS.gene008484.t1	MTR_1g077320	34.646	127	79	3	4	129	32	155	1.87e-19	79.3
MS.gene008484.t1	MTR_3g466890	36.000	125	79	1	2	126	29	152	4.83e-19	78.2
MS.gene008484.t1	MTR_3g466930	35.772	123	78	1	4	126	2	123	6.32e-19	77.0
MS.gene008484.t1	MTR_5g047580	34.109	129	83	2	1	129	29	155	6.65e-18	75.1
MS.gene008484.t1	MTR_1g077300	31.538	130	85	2	4	133	32	157	1.22e-17	74.3
MS.gene008484.t1	MTR_4g028720	32.540	126	83	2	4	129	32	155	1.71e-17	74.3
MS.gene008484.t1	MTR_7g011950	31.746	126	84	2	4	129	32	155	2.46e-17	73.6
MS.gene008484.t1	MTR_1g077390	35.606	132	77	4	1	129	29	155	9.97e-17	72.0
MS.gene008484.t1	MTR_7g055800	42.857	84	33	1	3	86	18	86	1.56e-16	69.7
MS.gene008484.t1	MTR_1g090783	31.008	129	87	2	1	129	28	154	1.63e-16	71.6
MS.gene008484.t1	MTR_1g084950	30.709	127	84	3	4	129	32	155	1.96e-15	68.6
MS.gene008484.t1	MTR_7g055940	42.105	76	29	1	3	78	18	78	4.78e-14	63.2
MS.gene008484.t1	MTR_1g090697	29.921	127	85	3	1	126	29	152	4.66e-13	62.4
MS.gene008484.t1	MTR_4g084780	55.556	54	24	0	46	99	1	54	2.70e-12	60.5
MS.gene008484.t1	MTR_1g090710	28.906	128	86	3	1	126	29	153	4.91e-12	59.7
MS.gene008484.t1	MTR_4g028800	48.889	45	23	0	1	45	27	71	8.77e-12	57.0
MS.gene008484.t1	MTR_4g019670	35.227	88	57	0	3	90	34	121	9.02e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008484.t1	AT5G48670	45.600	125	65	2	4	127	32	154	1.05e-29	109
MS.gene008484.t1	AT5G27810	53.933	89	41	0	1	89	1	89	6.10e-29	102
MS.gene008484.t1	AT1G65300	39.394	132	78	2	2	133	29	158	3.02e-23	91.7
MS.gene008484.t1	AT1G65330	38.525	122	74	1	1	122	28	148	3.69e-20	83.6
MS.gene008484.t1	AT3G05860	38.710	124	71	2	1	122	29	149	1.64e-19	80.5
MS.gene008484.t1	AT3G05860	38.710	124	71	2	1	122	29	149	6.01e-19	80.1
MS.gene008484.t1	AT3G05860	38.710	124	71	2	1	122	29	149	7.53e-19	79.7
MS.gene008484.t1	AT5G26650	33.333	129	85	1	1	129	27	154	5.14e-17	75.9
MS.gene008484.t1	AT5G26630	30.579	121	83	1	6	126	34	153	4.75e-16	71.6
MS.gene008484.t1	AT1G31640	36.364	132	70	4	2	127	29	152	4.56e-15	70.9
MS.gene008484.t1	AT5G27960	31.008	129	88	1	1	129	27	154	6.16e-15	70.1
MS.gene008484.t1	AT2G28700	30.328	122	84	1	1	122	29	149	2.38e-12	62.8
MS.gene008484.t1	AT1G22590	29.545	132	76	2	1	129	29	146	6.17e-12	59.7
MS.gene008484.t1	AT1G31630	31.343	134	82	5	3	133	30	156	2.91e-11	59.7

Find 29 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGGTCATTTCCTGCTTTC+TGG	0.066665	3.3:+55599905	None:intergenic
ATTCATACCACAGAATTTAA+TGG	0.196701	3.3:-55599867	MS.gene008484:CDS
CAGGTTTGGCCATCAGATTC+AGG	0.224706	3.3:-55600062	MS.gene008484:CDS
GTTCAGGAGTTTGCATGAAC+TGG	0.380322	3.3:-55600018	MS.gene008484:CDS
GATGAAATTAGCACTCTTTG+TGG	0.382102	3.3:-55600131	MS.gene008484:CDS
CTGAATCTGATGGCCAAACC+TGG	0.394792	3.3:+55600063	None:intergenic
GAAGTGTGATATCCAGGTTC+AGG	0.406864	3.3:-55600034	MS.gene008484:CDS
AATGAAATGATGAATGATAT+TGG	0.442815	3.3:+55599887	None:intergenic
AGGTTTGGCCATCAGATTCA+GGG	0.444978	3.3:-55600061	MS.gene008484:CDS
ACAATCCTCAAGCCCAGGTT+TGG	0.451431	3.3:-55600076	MS.gene008484:CDS
TATTCTTGAGGCAAAGCATT+GGG	0.452810	3.3:-55599965	MS.gene008484:CDS
GAGGACAAATCATTAAGATC+AGG	0.474701	3.3:+55599828	None:intergenic
GGGTTAGAAGTGTGATATCC+AGG	0.506627	3.3:-55600040	MS.gene008484:CDS
AAGGAAGGAAACCAGAAAGC+AGG	0.520416	3.3:-55599916	MS.gene008484:CDS
CAAATTCTCAGCAATAAAAG+AGG	0.520681	3.3:+55599809	None:intergenic
TTCATGCAAACTCCTGAACC+TGG	0.526718	3.3:+55600022	None:intergenic
ACTCTTTGTGGAATAGAAGC+TGG	0.543874	3.3:-55600119	MS.gene008484:CDS
TAAATATCCATTAAATTCTG+TGG	0.554522	3.3:+55599860	None:intergenic
GTATTCTTGAGGCAAAGCAT+TGG	0.555152	3.3:-55599966	MS.gene008484:CDS
GATGGCCAAACCTGGGCTTG+AGG	0.558655	3.3:+55600071	None:intergenic
GCAAAACAATCCTCAAGCCC+AGG	0.573782	3.3:-55600081	MS.gene008484:CDS
TGAATCTGATGGCCAAACCT+GGG	0.592740	3.3:+55600064	None:intergenic
GAGGTCGATCAAAAGATGAA+AGG	0.605953	3.3:-55599783	MS.gene008484:CDS
CTTCTAACCCCTGAATCTGA+TGG	0.608216	3.3:+55600053	None:intergenic
ATGAGCAGTTGATGAAACAA+AGG	0.608842	3.3:-55599935	MS.gene008484:CDS
GGTTTGGCCATCAGATTCAG+GGG	0.621539	3.3:-55600060	MS.gene008484:CDS
GCAGTTGATGAAACAAAGGA+AGG	0.627441	3.3:-55599931	MS.gene008484:CDS
TATTGCTGAGAATTTGAGGG+AGG	0.639350	3.3:-55599802	MS.gene008484:CDS
TGGATCAAGAGGTATTCTTG+AGG	0.709854	3.3:-55599977	MS.gene008484:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATGAAATGATGAATGATAT+TGG	+	chr3.3:55600023-55600042	None:intergenic	20.0%
!!	TAAATATCCATTAAATTCTG+TGG	+	chr3.3:55600050-55600069	None:intergenic	20.0%
!	ATTCATACCACAGAATTTAA+TGG	-	chr3.3:55600040-55600059	MS.gene008484:CDS	25.0%
!!	TTTTATTGCTGAGAATTTGA+GGG	-	chr3.3:55600102-55600121	MS.gene008484:CDS	25.0%
	ACTGGAACAAAGTAAAAAGA+TGG	-	chr3.3:55599907-55599926	MS.gene008484:CDS	30.0%
	CAAATTCTCAGCAATAAAAG+AGG	+	chr3.3:55600101-55600120	None:intergenic	30.0%
!	CTTTTATTGCTGAGAATTTG+AGG	-	chr3.3:55600101-55600120	MS.gene008484:CDS	30.0%
	GAGGACAAATCATTAAGATC+AGG	+	chr3.3:55600082-55600101	None:intergenic	35.0%
	GATGAAATTAGCACTCTTTG+TGG	-	chr3.3:55599776-55599795	MS.gene008484:CDS	35.0%
	GGAACAAAGTAAAAAGATGG+TGG	-	chr3.3:55599910-55599929	MS.gene008484:CDS	35.0%
!	TATTCTTGAGGCAAAGCATT+GGG	-	chr3.3:55599942-55599961	MS.gene008484:CDS	35.0%
!!	ATGAGCAGTTGATGAAACAA+AGG	-	chr3.3:55599972-55599991	MS.gene008484:CDS	35.0%
!!	TAAAAAGATGGTGGATCAAG+AGG	-	chr3.3:55599919-55599938	MS.gene008484:CDS	35.0%
	ACTCTTTGTGGAATAGAAGC+TGG	-	chr3.3:55599788-55599807	MS.gene008484:CDS	40.0%
	ATTGGTCATTTCCTGCTTTC+TGG	+	chr3.3:55600005-55600024	None:intergenic	40.0%
	GAGGTCGATCAAAAGATGAA+AGG	-	chr3.3:55600124-55600143	MS.gene008484:CDS	40.0%
	GTATTCTTGAGGCAAAGCAT+TGG	-	chr3.3:55599941-55599960	MS.gene008484:CDS	40.0%
	TATTGCTGAGAATTTGAGGG+AGG	-	chr3.3:55600105-55600124	MS.gene008484:CDS	40.0%
!!	GCAGTTGATGAAACAAAGGA+AGG	-	chr3.3:55599976-55599995	MS.gene008484:CDS	40.0%
!!	TGGATCAAGAGGTATTCTTG+AGG	-	chr3.3:55599930-55599949	MS.gene008484:CDS	40.0%
	AAGGAAGGAAACCAGAAAGC+AGG	-	chr3.3:55599991-55600010	MS.gene008484:CDS	45.0%
	AGGTTTGGCCATCAGATTCA+GGG	-	chr3.3:55599846-55599865	MS.gene008484:CDS	45.0%
	CTTCTAACCCCTGAATCTGA+TGG	+	chr3.3:55599857-55599876	None:intergenic	45.0%
	GAAGTGTGATATCCAGGTTC+AGG	-	chr3.3:55599873-55599892	MS.gene008484:CDS	45.0%
	GTTCAGGAGTTTGCATGAAC+TGG	-	chr3.3:55599889-55599908	MS.gene008484:CDS	45.0%
	TGAATCTGATGGCCAAACCT+GGG	+	chr3.3:55599846-55599865	None:intergenic	45.0%
	TTCATGCAAACTCCTGAACC+TGG	+	chr3.3:55599888-55599907	None:intergenic	45.0%
!	GGGTTAGAAGTGTGATATCC+AGG	-	chr3.3:55599867-55599886	MS.gene008484:CDS	45.0%
	ACAATCCTCAAGCCCAGGTT+TGG	-	chr3.3:55599831-55599850	MS.gene008484:CDS	50.0%
	CAGGTTTGGCCATCAGATTC+AGG	-	chr3.3:55599845-55599864	MS.gene008484:CDS	50.0%
	CTGAATCTGATGGCCAAACC+TGG	+	chr3.3:55599847-55599866	None:intergenic	50.0%
	GCAAAACAATCCTCAAGCCC+AGG	-	chr3.3:55599826-55599845	MS.gene008484:CDS	50.0%
	GGTTTGGCCATCAGATTCAG+GGG	-	chr3.3:55599847-55599866	MS.gene008484:CDS	50.0%
	GATGGCCAAACCTGGGCTTG+AGG	+	chr3.3:55599839-55599858	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	55599764	55600165	55599764	ID=MS.gene008484
chr3.3	mRNA	55599764	55600165	55599764	ID=MS.gene008484.t1;Parent=MS.gene008484
chr3.3	exon	55599764	55600165	55599764	ID=MS.gene008484.t1.exon1;Parent=MS.gene008484.t1
chr3.3	CDS	55599764	55600165	55599764	ID=cds.MS.gene008484.t1;Parent=MS.gene008484.t1

Gene Sequence

>MS.gene008484

ATGAAGAAGATTGATGAAATTAGCACTCTTTGTGGAATAGAAGCTGGTGCAATAATTTACGAGCAAAACAATCCTCAAGCCCAGGTTTGGCCATCAGATTCAGGGGTTAGAAGTGTGATATCCAGGTTCAGGAGTTTGCATGAACTGGAACAAAGTAAAAAGATGGTGGATCAAGAGGTATTCTTGAGGCAAAGCATTGGGAAAGTCTATGAGCAGTTGATGAAACAAAGGAAGGAAACCAGAAAGCAGGAAATGACCAATATCATTCATCATTTCATTCATACCACAGAATTTAATGGATATTTAATGAGCAAACCTGATCTTAATGATTTGTCCTCTTTTATTGCTGAGAATTTGAGGGAGGTCGATCAAAAGATGAAAGGAATGTTAATCGAAGATTGA

Protein sequence

>MS.gene008484.t1

MKKIDEISTLCGIEAGAIIYEQNNPQAQVWPSDSGVRSVISRFRSLHELEQSKKMVDQEVFLRQSIGKVYEQLMKQRKETRKQEMTNIIHHFIHTTEFNGYLMSKPDLNDLSSFIAENLREVDQKMKGMLIED