Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008485.t1 | KEH34477.1 | 86.5 | 133 | 18 | 0 | 1 | 133 | 29 | 161 | 3.70E-55 | 224.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008485.t1 | Q9FJK3 | 44.1 | 127 | 70 | 1 | 1 | 127 | 29 | 154 | 1.6e-22 | 107.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008485.t1 | A0A072UY35 | 86.5 | 133 | 18 | 0 | 1 | 133 | 29 | 161 | 2.6e-55 | 224.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene008485.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008485.t1 | MTR_3g467080 | 86.466 | 133 | 18 | 0 | 1 | 133 | 29 | 161 | 8.61e-82 | 237 |
| MS.gene008485.t1 | MTR_4g063790 | 76.692 | 133 | 31 | 0 | 1 | 133 | 29 | 161 | 2.77e-71 | 211 |
| MS.gene008485.t1 | MTR_3g465410 | 75.940 | 133 | 32 | 0 | 1 | 133 | 1 | 133 | 7.60e-70 | 206 |
| MS.gene008485.t1 | MTR_3g466980 | 51.587 | 126 | 60 | 1 | 1 | 126 | 29 | 153 | 5.82e-41 | 135 |
| MS.gene008485.t1 | MTR_3g031100 | 45.455 | 132 | 72 | 0 | 1 | 132 | 29 | 160 | 7.01e-35 | 121 |
| MS.gene008485.t1 | MTR_3g065100 | 45.528 | 123 | 66 | 1 | 4 | 126 | 32 | 153 | 6.28e-34 | 118 |
| MS.gene008485.t1 | MTR_3g031240 | 46.512 | 129 | 69 | 0 | 1 | 129 | 29 | 157 | 4.25e-32 | 112 |
| MS.gene008485.t1 | MTR_4g032290 | 42.308 | 130 | 75 | 0 | 4 | 133 | 32 | 161 | 2.46e-31 | 111 |
| MS.gene008485.t1 | MTR_2g016210 | 40.909 | 132 | 78 | 0 | 1 | 132 | 29 | 160 | 1.97e-30 | 109 |
| MS.gene008485.t1 | MTR_2g035580 | 47.200 | 125 | 65 | 1 | 2 | 126 | 30 | 153 | 3.09e-30 | 106 |
| MS.gene008485.t1 | MTR_4g032260 | 39.024 | 123 | 75 | 0 | 6 | 128 | 34 | 156 | 9.65e-30 | 105 |
| MS.gene008485.t1 | MTR_4g031910 | 41.600 | 125 | 73 | 0 | 4 | 128 | 32 | 156 | 2.81e-29 | 106 |
| MS.gene008485.t1 | MTR_4g032620 | 36.508 | 126 | 80 | 0 | 1 | 126 | 29 | 154 | 1.50e-27 | 102 |
| MS.gene008485.t1 | MTR_2g035610 | 48.352 | 91 | 47 | 0 | 4 | 94 | 32 | 122 | 8.69e-27 | 97.1 |
| MS.gene008485.t1 | MTR_5g075380 | 36.508 | 126 | 79 | 1 | 1 | 126 | 29 | 153 | 1.86e-26 | 97.4 |
| MS.gene008485.t1 | MTR_8g036130 | 39.695 | 131 | 77 | 1 | 1 | 129 | 29 | 159 | 1.23e-25 | 97.4 |
| MS.gene008485.t1 | MTR_3g466830 | 40.000 | 125 | 74 | 1 | 1 | 125 | 28 | 151 | 2.11e-24 | 92.0 |
| MS.gene008485.t1 | MTR_3g466900 | 34.921 | 126 | 81 | 1 | 4 | 129 | 2 | 126 | 5.06e-21 | 82.4 |
| MS.gene008485.t1 | MTR_1g077320 | 33.858 | 127 | 80 | 3 | 4 | 129 | 32 | 155 | 1.96e-19 | 79.3 |
| MS.gene008485.t1 | MTR_3g466890 | 36.000 | 125 | 79 | 1 | 2 | 126 | 29 | 152 | 2.23e-19 | 79.0 |
| MS.gene008485.t1 | MTR_3g466930 | 35.772 | 123 | 78 | 1 | 4 | 126 | 2 | 123 | 3.38e-19 | 77.8 |
| MS.gene008485.t1 | MTR_5g047580 | 33.333 | 129 | 84 | 2 | 1 | 129 | 29 | 155 | 1.09e-17 | 74.7 |
| MS.gene008485.t1 | MTR_1g077300 | 30.769 | 130 | 86 | 2 | 4 | 133 | 32 | 157 | 1.50e-17 | 74.3 |
| MS.gene008485.t1 | MTR_4g028720 | 31.746 | 126 | 84 | 2 | 4 | 129 | 32 | 155 | 3.08e-17 | 73.6 |
| MS.gene008485.t1 | MTR_7g011950 | 30.952 | 126 | 85 | 2 | 4 | 129 | 32 | 155 | 4.11e-17 | 73.2 |
| MS.gene008485.t1 | MTR_7g055800 | 42.857 | 84 | 33 | 1 | 3 | 86 | 18 | 86 | 1.65e-16 | 69.7 |
| MS.gene008485.t1 | MTR_1g077390 | 33.333 | 129 | 84 | 2 | 1 | 129 | 29 | 155 | 2.11e-16 | 71.2 |
| MS.gene008485.t1 | MTR_1g090783 | 30.233 | 129 | 88 | 2 | 1 | 129 | 28 | 154 | 4.26e-16 | 70.5 |
| MS.gene008485.t1 | MTR_1g084950 | 29.921 | 127 | 85 | 3 | 4 | 129 | 32 | 155 | 4.90e-15 | 67.8 |
| MS.gene008485.t1 | MTR_7g055940 | 42.105 | 76 | 29 | 1 | 3 | 78 | 18 | 78 | 4.88e-14 | 63.2 |
| MS.gene008485.t1 | MTR_1g090697 | 29.134 | 127 | 86 | 3 | 1 | 126 | 29 | 152 | 5.53e-13 | 62.4 |
| MS.gene008485.t1 | MTR_1g090710 | 27.481 | 131 | 90 | 3 | 1 | 129 | 29 | 156 | 5.57e-12 | 59.7 |
| MS.gene008485.t1 | MTR_4g084780 | 53.704 | 54 | 25 | 0 | 46 | 99 | 1 | 54 | 5.97e-12 | 59.7 |
| MS.gene008485.t1 | MTR_4g028800 | 48.889 | 45 | 23 | 0 | 1 | 45 | 27 | 71 | 7.39e-12 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene008485.t1 | AT5G48670 | 44.000 | 125 | 67 | 2 | 4 | 127 | 32 | 154 | 6.02e-29 | 107 |
| MS.gene008485.t1 | AT5G27810 | 51.685 | 89 | 43 | 0 | 1 | 89 | 1 | 89 | 4.94e-28 | 100 |
| MS.gene008485.t1 | AT1G65300 | 38.636 | 132 | 79 | 2 | 2 | 133 | 29 | 158 | 1.77e-23 | 92.4 |
| MS.gene008485.t1 | AT1G65330 | 37.705 | 122 | 75 | 1 | 1 | 122 | 28 | 148 | 4.14e-20 | 83.6 |
| MS.gene008485.t1 | AT3G05860 | 38.710 | 124 | 71 | 2 | 1 | 122 | 29 | 149 | 1.18e-19 | 80.9 |
| MS.gene008485.t1 | AT3G05860 | 45.161 | 93 | 47 | 1 | 1 | 89 | 29 | 121 | 2.54e-19 | 80.9 |
| MS.gene008485.t1 | AT3G05860 | 45.161 | 93 | 47 | 1 | 1 | 89 | 29 | 121 | 3.59e-19 | 80.9 |
| MS.gene008485.t1 | AT5G26650 | 32.558 | 129 | 86 | 1 | 1 | 129 | 27 | 154 | 6.02e-17 | 75.9 |
| MS.gene008485.t1 | AT5G26630 | 31.405 | 121 | 82 | 1 | 6 | 126 | 34 | 153 | 1.38e-16 | 73.2 |
| MS.gene008485.t1 | AT1G31640 | 35.606 | 132 | 71 | 4 | 2 | 127 | 29 | 152 | 3.64e-15 | 71.2 |
| MS.gene008485.t1 | AT5G27960 | 30.233 | 129 | 89 | 1 | 1 | 129 | 27 | 154 | 7.76e-15 | 69.7 |
| MS.gene008485.t1 | AT2G28700 | 30.328 | 122 | 84 | 1 | 1 | 122 | 29 | 149 | 5.97e-13 | 64.3 |
| MS.gene008485.t1 | AT1G22590 | 28.788 | 132 | 77 | 2 | 1 | 129 | 29 | 146 | 3.38e-12 | 60.5 |
| MS.gene008485.t1 | AT1G31630 | 31.655 | 139 | 75 | 6 | 3 | 133 | 30 | 156 | 1.15e-11 | 60.8 |
| MS.gene008485.t1 | AT5G06500 | 31.092 | 119 | 72 | 3 | 1 | 119 | 29 | 137 | 8.96e-11 | 57.8 |
Find 28 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGTCATTTCCTGCTTTC+TGG | 0.066665 | 3.3:-55592918 | None:intergenic |
| ATTCATACCACAGAATTTAA+TGG | 0.196701 | 3.3:+55592956 | MS.gene008485:CDS |
| CAGGTTTGGCCATCAGATTC+AGG | 0.224706 | 3.3:+55592761 | MS.gene008485:CDS |
| GTTCAAGAGTTTGCATGAAC+TGG | 0.380322 | 3.3:+55592805 | MS.gene008485:CDS |
| CTGAATCTGATGGCCAAACC+TGG | 0.394792 | 3.3:-55592760 | None:intergenic |
| GATGAAATTAGCACTCTTTG+CGG | 0.413383 | 3.3:+55592692 | MS.gene008485:CDS |
| AATGAAATGATGAATGATAT+TGG | 0.442815 | 3.3:-55592936 | None:intergenic |
| AGGTTTGGCCATCAGATTCA+GGG | 0.444978 | 3.3:+55592762 | MS.gene008485:CDS |
| ACAATCCTCAAGCCCAGGTT+TGG | 0.451431 | 3.3:+55592747 | MS.gene008485:CDS |
| TATTCTTGAGGCAAAGCATT+GGG | 0.452810 | 3.3:+55592858 | MS.gene008485:CDS |
| GGGTTAGAAGTGTGATATCC+AGG | 0.506627 | 3.3:+55592783 | MS.gene008485:CDS |
| AAGGAAGGAAACCAGAAAGC+AGG | 0.520416 | 3.3:+55592907 | MS.gene008485:CDS |
| CAAATTCTCAGCAATAAAAG+AGG | 0.520681 | 3.3:-55593014 | None:intergenic |
| TAAATATCCATTAAATTCTG+TGG | 0.554522 | 3.3:-55592963 | None:intergenic |
| GTATTCTTGAGGCAAAGCAT+TGG | 0.555152 | 3.3:+55592857 | MS.gene008485:CDS |
| GATGGCCAAACCTGGGCTTG+AGG | 0.558655 | 3.3:-55592752 | None:intergenic |
| TTGCTCGTAAATTATTGCAC+CGG | 0.562136 | 3.3:-55592723 | None:intergenic |
| GCAAAACAATCCTCAAGCCC+AGG | 0.573782 | 3.3:+55592742 | MS.gene008485:CDS |
| TTCATGCAAACTCTTGAACC+TGG | 0.581655 | 3.3:-55592801 | None:intergenic |
| TGAATCTGATGGCCAAACCT+GGG | 0.592740 | 3.3:-55592759 | None:intergenic |
| GAGGTCGATCAAAAGATGAA+AGG | 0.605953 | 3.3:+55593040 | MS.gene008485:CDS |
| CTTCTAACCCCTGAATCTGA+TGG | 0.608216 | 3.3:-55592770 | None:intergenic |
| ATGAGCAGATGATGAAACAA+AGG | 0.611511 | 3.3:+55592888 | MS.gene008485:CDS |
| ACTCTTTGCGGAATAGAAGC+CGG | 0.612299 | 3.3:+55592704 | MS.gene008485:CDS |
| GGTTTGGCCATCAGATTCAG+GGG | 0.621539 | 3.3:+55592763 | MS.gene008485:CDS |
| TATTGCTGAGAATTTGAGGG+AGG | 0.639350 | 3.3:+55593021 | MS.gene008485:CDS |
| GCAGATGATGAAACAAAGGA+AGG | 0.642572 | 3.3:+55592892 | MS.gene008485:CDS |
| TGGATCAAGAGGTATTCTTG+AGG | 0.709854 | 3.3:+55592846 | MS.gene008485:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAATGATGAATGATAT+TGG | - | chr3.3:55592939-55592958 | None:intergenic | 20.0% |
| !! | TAAATATCCATTAAATTCTG+TGG | - | chr3.3:55592966-55592985 | None:intergenic | 20.0% |
| ! | ATTCATACCACAGAATTTAA+TGG | + | chr3.3:55592956-55592975 | MS.gene008485:CDS | 25.0% |
| !! | TTTTATTGCTGAGAATTTGA+GGG | + | chr3.3:55593018-55593037 | MS.gene008485:CDS | 25.0% |
| ACTGGAACAAAGTAAAAAGA+TGG | + | chr3.3:55592823-55592842 | MS.gene008485:CDS | 30.0% | |
| CAAATTCTCAGCAATAAAAG+AGG | - | chr3.3:55593017-55593036 | None:intergenic | 30.0% | |
| ! | CTTTTATTGCTGAGAATTTG+AGG | + | chr3.3:55593017-55593036 | MS.gene008485:CDS | 30.0% |
| ATGAGCAGATGATGAAACAA+AGG | + | chr3.3:55592888-55592907 | MS.gene008485:CDS | 35.0% | |
| GATGAAATTAGCACTCTTTG+CGG | + | chr3.3:55592692-55592711 | MS.gene008485:CDS | 35.0% | |
| GGAACAAAGTAAAAAGATGG+TGG | + | chr3.3:55592826-55592845 | MS.gene008485:CDS | 35.0% | |
| TTGCTCGTAAATTATTGCAC+CGG | - | chr3.3:55592726-55592745 | None:intergenic | 35.0% | |
| ! | TATTCTTGAGGCAAAGCATT+GGG | + | chr3.3:55592858-55592877 | MS.gene008485:CDS | 35.0% |
| !! | TAAAAAGATGGTGGATCAAG+AGG | + | chr3.3:55592835-55592854 | MS.gene008485:CDS | 35.0% |
| ATTGGTCATTTCCTGCTTTC+TGG | - | chr3.3:55592921-55592940 | None:intergenic | 40.0% | |
| GAGGTCGATCAAAAGATGAA+AGG | + | chr3.3:55593040-55593059 | MS.gene008485:CDS | 40.0% | |
| GCAGATGATGAAACAAAGGA+AGG | + | chr3.3:55592892-55592911 | MS.gene008485:CDS | 40.0% | |
| GTATTCTTGAGGCAAAGCAT+TGG | + | chr3.3:55592857-55592876 | MS.gene008485:CDS | 40.0% | |
| GTTCAAGAGTTTGCATGAAC+TGG | + | chr3.3:55592805-55592824 | MS.gene008485:CDS | 40.0% | |
| TATTGCTGAGAATTTGAGGG+AGG | + | chr3.3:55593021-55593040 | MS.gene008485:CDS | 40.0% | |
| TTCATGCAAACTCTTGAACC+TGG | - | chr3.3:55592804-55592823 | None:intergenic | 40.0% | |
| !! | TGGATCAAGAGGTATTCTTG+AGG | + | chr3.3:55592846-55592865 | MS.gene008485:CDS | 40.0% |
| AAGGAAGGAAACCAGAAAGC+AGG | + | chr3.3:55592907-55592926 | MS.gene008485:CDS | 45.0% | |
| ACTCTTTGCGGAATAGAAGC+CGG | + | chr3.3:55592704-55592723 | MS.gene008485:CDS | 45.0% | |
| AGGTTTGGCCATCAGATTCA+GGG | + | chr3.3:55592762-55592781 | MS.gene008485:CDS | 45.0% | |
| CTTCTAACCCCTGAATCTGA+TGG | - | chr3.3:55592773-55592792 | None:intergenic | 45.0% | |
| TGAATCTGATGGCCAAACCT+GGG | - | chr3.3:55592762-55592781 | None:intergenic | 45.0% | |
| ! | GGGTTAGAAGTGTGATATCC+AGG | + | chr3.3:55592783-55592802 | MS.gene008485:CDS | 45.0% |
| ACAATCCTCAAGCCCAGGTT+TGG | + | chr3.3:55592747-55592766 | MS.gene008485:CDS | 50.0% | |
| CAGGTTTGGCCATCAGATTC+AGG | + | chr3.3:55592761-55592780 | MS.gene008485:CDS | 50.0% | |
| CTGAATCTGATGGCCAAACC+TGG | - | chr3.3:55592763-55592782 | None:intergenic | 50.0% | |
| GCAAAACAATCCTCAAGCCC+AGG | + | chr3.3:55592742-55592761 | MS.gene008485:CDS | 50.0% | |
| GGTTTGGCCATCAGATTCAG+GGG | + | chr3.3:55592763-55592782 | MS.gene008485:CDS | 50.0% | |
| GATGGCCAAACCTGGGCTTG+AGG | - | chr3.3:55592755-55592774 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 55592680 | 55593081 | 55592680 | ID=MS.gene008485 |
| chr3.3 | mRNA | 55592680 | 55593081 | 55592680 | ID=MS.gene008485.t1;Parent=MS.gene008485 |
| chr3.3 | exon | 55592680 | 55593081 | 55592680 | ID=MS.gene008485.t1.exon1;Parent=MS.gene008485.t1 |
| chr3.3 | CDS | 55592680 | 55593081 | 55592680 | ID=cds.MS.gene008485.t1;Parent=MS.gene008485.t1 |
| Gene Sequence |
| Protein sequence |
