AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008648


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008648.t1 MTR_4g063790 89.474 171 18 0 1 171 1 171 2.88e-110 311
MS.gene008648.t1 MTR_3g465410 92.308 143 11 0 29 171 1 143 5.98e-96 274
MS.gene008648.t1 MTR_3g467080 78.261 161 35 0 1 161 1 161 2.40e-89 258
MS.gene008648.t1 MTR_3g466980 54.335 173 74 3 1 170 1 171 1.42e-58 182
MS.gene008648.t1 MTR_3g031240 49.693 163 82 0 1 163 1 163 2.36e-51 162
MS.gene008648.t1 MTR_2g016210 46.746 169 90 0 1 169 1 169 1.93e-50 162
MS.gene008648.t1 MTR_3g065100 50.633 158 77 1 1 158 1 157 3.25e-44 146
MS.gene008648.t1 MTR_3g031100 43.195 169 96 0 1 169 1 169 7.89e-43 143
MS.gene008648.t1 MTR_4g032620 40.606 165 98 0 1 165 1 165 1.40e-41 139
MS.gene008648.t1 MTR_5g075380 40.625 160 88 2 1 157 1 156 1.86e-40 134
MS.gene008648.t1 MTR_8g036130 41.916 167 95 1 1 165 1 167 8.21e-40 135
MS.gene008648.t1 MTR_2g035580 45.806 155 83 1 1 155 1 154 2.34e-37 126
MS.gene008648.t1 MTR_4g031910 42.424 165 95 0 1 165 1 165 5.54e-37 127
MS.gene008648.t1 MTR_5g047580 41.139 158 91 2 1 158 1 156 7.21e-36 122
MS.gene008648.t1 MTR_4g032290 42.424 165 95 0 1 165 1 165 3.62e-35 123
MS.gene008648.t1 MTR_3g466830 40.000 155 92 1 3 157 2 155 1.19e-34 119
MS.gene008648.t1 MTR_1g077390 42.138 159 90 2 1 159 1 157 1.41e-34 119
MS.gene008648.t1 MTR_4g032260 40.252 159 95 0 1 159 1 159 1.23e-33 117
MS.gene008648.t1 MTR_2g035610 48.718 117 60 0 1 117 1 117 1.19e-32 113
MS.gene008648.t1 MTR_1g084950 41.139 158 91 2 1 158 1 156 6.74e-32 112
MS.gene008648.t1 MTR_1g077320 41.509 159 91 2 1 159 1 157 7.02e-32 112
MS.gene008648.t1 MTR_4g028720 41.509 159 91 2 1 159 1 157 9.20e-32 112
MS.gene008648.t1 MTR_1g090783 38.816 152 91 2 3 154 2 151 2.06e-31 111
MS.gene008648.t1 MTR_7g011950 40.881 159 92 2 1 159 1 157 5.31e-31 110
MS.gene008648.t1 MTR_1g077300 40.994 161 91 2 1 161 1 157 2.39e-30 108
MS.gene008648.t1 MTR_1g090710 36.364 154 97 1 1 154 1 153 7.85e-29 104
MS.gene008648.t1 MTR_3g466890 37.419 155 96 1 3 157 2 155 1.15e-28 104
MS.gene008648.t1 MTR_1g090697 36.538 156 93 3 1 154 1 152 1.98e-28 103
MS.gene008648.t1 MTR_5g047560 35.714 154 81 3 1 154 1 136 1.34e-21 85.5
MS.gene008648.t1 MTR_3g466930 34.921 126 81 1 32 157 2 126 4.72e-20 81.3
MS.gene008648.t1 MTR_3g466900 34.127 126 82 1 32 157 2 126 8.16e-20 80.9
MS.gene008648.t1 MTR_4g028800 47.945 73 36 1 1 73 1 71 1.10e-19 78.6
MS.gene008648.t1 MTR_7g055800 35.897 117 47 2 1 117 1 89 2.81e-16 70.5
MS.gene008648.t1 MTR_4g019670 30.625 160 100 4 1 154 1 155 3.65e-16 75.5
MS.gene008648.t1 MTR_7g055790 39.450 109 48 3 1 109 59 149 2.01e-15 69.7
MS.gene008648.t1 MTR_7g106510 30.282 142 87 3 1 139 1 133 2.32e-15 73.2
MS.gene008648.t1 MTR_7g055940 35.849 106 40 2 1 106 1 78 2.94e-14 64.7
MS.gene008648.t1 MTR_3g093900 32.323 99 67 0 5 103 8 106 2.67e-13 67.0
MS.gene008648.t1 MTR_6g018920 26.250 160 103 4 1 154 1 151 2.99e-13 66.6
MS.gene008648.t1 MTR_6g005440 28.750 160 99 4 1 154 1 151 5.64e-12 62.8
MS.gene008648.t1 MTR_1g114730 25.786 159 103 4 1 153 1 150 3.01e-11 60.8
MS.gene008648.t1 MTR_4g084780 55.556 54 24 0 74 127 1 54 8.08e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008648.t1 AT5G48670 51.592 157 71 3 1 155 1 154 6.13e-41 140
MS.gene008648.t1 AT3G05860 42.763 152 82 2 1 150 1 149 1.52e-32 115
MS.gene008648.t1 AT3G05860 39.535 172 99 2 1 170 1 169 3.67e-32 115
MS.gene008648.t1 AT3G05860 39.535 172 99 2 1 170 1 169 4.72e-32 115
MS.gene008648.t1 AT1G65330 40.523 153 90 1 3 155 2 153 7.82e-31 113
MS.gene008648.t1 AT5G27810 53.933 89 41 0 29 117 1 89 6.88e-30 106
MS.gene008648.t1 AT1G65300 38.509 161 97 2 3 163 2 160 1.58e-28 107
MS.gene008648.t1 AT5G26650 44.954 109 60 0 5 113 3 111 1.85e-26 103
MS.gene008648.t1 AT5G27960 44.037 109 61 0 5 113 3 111 8.17e-26 100
MS.gene008648.t1 AT1G31630 36.667 150 91 3 3 151 2 148 1.45e-25 100
MS.gene008648.t1 AT1G22590 39.823 113 68 0 1 113 1 113 1.14e-24 94.4
MS.gene008648.t1 AT1G31640 38.994 159 83 5 3 155 2 152 2.82e-24 98.2
MS.gene008648.t1 AT5G26630 36.364 154 97 1 1 154 1 153 2.08e-23 92.4
MS.gene008648.t1 AT2G28700 30.519 154 82 1 1 129 1 154 2.55e-20 86.3
MS.gene008648.t1 AT5G06500 36.937 111 66 1 1 111 1 107 8.95e-20 83.2
MS.gene008648.t1 AT2G40210 36.634 101 64 0 1 101 1 101 1.10e-14 70.9
MS.gene008648.t1 AT5G26580 37.255 102 50 1 1 102 1 88 7.78e-14 68.6

Find 42 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCAAACCATAGAATTTAA+TGG 0.173791 3.4:-60427172 MS.gene008648:CDS
AGTGGTCATTTCCATCTTTC+TGG 0.183128 3.4:+60427210 None:intergenic
CGAATTTCCATTAAATTCTA+TGG 0.218755 3.4:+60427165 None:intergenic
GAGGTTTGGCCATCAGATTC+AGG 0.258145 3.4:-60427367 MS.gene008648:CDS
AAAATCATTAAGATCATCTT+TGG 0.313774 3.4:+60427138 None:intergenic
CGCTACCATGACTAGAAATA+AGG 0.322931 3.4:-60427760 None:intergenic
ATCAAGAGGAGGTTGGAAAT+GGG 0.388178 3.4:-60427051 MS.gene008648:CDS
GATCAAGAGGAGGTTGGAAA+TGG 0.411170 3.4:-60427052 MS.gene008648:CDS
GAAGTGTGATATCTAGGTTT+AGG 0.426765 3.4:-60427339 MS.gene008648:CDS
GGGTCAGAAGTGTGATATCT+AGG 0.436600 3.4:-60427345 MS.gene008648:CDS
TCTAGGTTTAGGAGTTTGCC+TGG 0.445921 3.4:-60427328 MS.gene008648:CDS
TCTTCTTGAGGCAAAGCATT+GGG 0.447886 3.4:-60427270 MS.gene008648:CDS
AGAATTTGAAGGAGGTTGAT+CGG 0.478161 3.4:-60427099 MS.gene008648:CDS
ACAACCCTCGAGTAGAGGTT+TGG 0.487846 3.4:-60427381 MS.gene008648:CDS
GTCTTCTTGAGGCAAAGCAT+TGG 0.498020 3.4:-60427271 MS.gene008648:CDS
TCAAGAGGAGGTTGGAAATG+GGG 0.500358 3.4:-60427050 MS.gene008648:CDS
TGATCGGAAGATGAAAGAAA+TGG 0.504501 3.4:-60427083 MS.gene008648:CDS
AGGTTTGGCCATCAGATTCA+GGG 0.511068 3.4:-60427366 MS.gene008648:CDS
ACGAGCAGTTGAAGAAGCAA+AGG 0.512962 3.4:-60427240 MS.gene008648:CDS
GGAGGATAACATACAGAAAG+AGG 0.518632 3.4:-60427704 MS.gene008648:CDS
TTTCACCTTATTTCTAGTCA+TGG 0.525434 3.4:+60427755 None:intergenic
AATATAATGATCAATGATAG+TGG 0.535373 3.4:+60427192 None:intergenic
AAGGGAGGATACCAGAAAGA+TGG 0.535884 3.4:-60427221 MS.gene008648:CDS
ATCAAAGATCAAGAGGAGGT+TGG 0.551560 3.4:-60427058 MS.gene008648:CDS
GAGGAGGTTGGAAATGGGGT+GGG 0.563227 3.4:-60427046 MS.gene008648:CDS
ACATGCTTCGACTCCACATA+GGG 0.565466 3.4:+60427423 None:intergenic
AGAGGAGGTTGGAAATGGGG+TGG 0.565910 3.4:-60427047 MS.gene008648:CDS
CGAGCAGTTGAAGAAGCAAA+GGG 0.580872 3.4:-60427239 MS.gene008648:CDS
AATGGCAATCAAAGATCAAG+AGG 0.588543 3.4:-60427065 MS.gene008648:CDS
TATTGATGAGAATTTGAAGG+AGG 0.592173 3.4:-60427107 MS.gene008648:CDS
AATGAAATTAGCACCCTATG+TGG 0.604835 3.4:-60427436 MS.gene008648:CDS
GCAAAACAACCCTCGAGTAG+AGG 0.609086 3.4:-60427386 MS.gene008648:CDS
CACATGCTTCGACTCCACAT+AGG 0.615585 3.4:+60427422 None:intergenic
ACATAACATGTGACTCGAAG+AGG 0.616371 3.4:-60427725 MS.gene008648:CDS
CTTCTGACCCCTGAATCTGA+TGG 0.624024 3.4:+60427358 None:intergenic
GATGGCCAAACCTCTACTCG+AGG 0.634480 3.4:+60427376 None:intergenic
GGCAATCAAAGATCAAGAGG+AGG 0.656411 3.4:-60427062 MS.gene008648:CDS
GGTTTGGCCATCAGATTCAG+GGG 0.657992 3.4:-60427365 MS.gene008648:CDS
ATGGCCAAACCTCTACTCGA+GGG 0.659257 3.4:+60427377 None:intergenic
TAACATGTGACTCGAAGAGG+AGG 0.659370 3.4:-60427722 MS.gene008648:CDS
GCAGTTGAAGAAGCAAAGGG+AGG 0.685834 3.4:-60427236 MS.gene008648:CDS
TGGATCAGGAGGTCTTCTTG+AGG 0.700337 3.4:-60427282 MS.gene008648:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATCATTAAGATCATCTT+TGG + chr3.4:60427657-60427676 None:intergenic 20.0%
!! AATATAATGATCAATGATAG+TGG + chr3.4:60427603-60427622 None:intergenic 20.0%
!! ATTCAAACCATAGAATTTAA+TGG - chr3.4:60427620-60427639 MS.gene008648:intron 20.0%
!!! AAAAAATGTGTGTTATTTCT+AGG + chr3.4:60427197-60427216 None:intergenic 20.0%
!!! TACATGCTTTATTGAAATTA+TGG + chr3.4:60427137-60427156 None:intergenic 20.0%
!!! TTTTATTGATGAGAATTTGA+AGG - chr3.4:60427682-60427701 MS.gene008648:intron 20.0%
! AATGAACAATATACAATCGA+TGG + chr3.4:60427296-60427315 None:intergenic 25.0%
! ACATGAACAAAGTAAAAAGA+TGG - chr3.4:60427487-60427506 MS.gene008648:intron 25.0%
! CGAATTTCCATTAAATTCTA+TGG + chr3.4:60427630-60427649 None:intergenic 25.0%
!!! TGTGTTATTTCTAGGAAAAA+AGG + chr3.4:60427189-60427208 None:intergenic 25.0%
ACATACAGAAAGAGGAAAAA+TGG - chr3.4:60427096-60427115 MS.gene008648:CDS 30.0%
TATTGATGAGAATTTGAAGG+AGG - chr3.4:60427685-60427704 MS.gene008648:intron 30.0%
TGAACAAAGTAAAAAGATGG+TGG - chr3.4:60427490-60427509 MS.gene008648:intron 30.0%
! GCTTTATTGAAATTATGGAG+AGG + chr3.4:60427132-60427151 None:intergenic 30.0%
! TTTTTACTTTGTTCATGTCC+AGG + chr3.4:60427485-60427504 None:intergenic 30.0%
!!! GTTGTTTTGCTCTGAAATTA+TGG + chr3.4:60427396-60427415 None:intergenic 30.0%
AATGAAATTAGCACCCTATG+TGG - chr3.4:60427356-60427375 MS.gene008648:CDS 35.0%
AATGGCAATCAAAGATCAAG+AGG - chr3.4:60427727-60427746 MS.gene008648:CDS 35.0%
GAAGTGTGATATCTAGGTTT+AGG - chr3.4:60427453-60427472 MS.gene008648:CDS 35.0%
TGATCGGAAGATGAAAGAAA+TGG - chr3.4:60427709-60427728 MS.gene008648:CDS 35.0%
! AGAATTTGAAGGAGGTTGAT+CGG - chr3.4:60427693-60427712 MS.gene008648:intron 35.0%
!! AAGTAAAAAGATGGTGGATC+AGG - chr3.4:60427496-60427515 MS.gene008648:intron 35.0%
ACATAACATGTGACTCGAAG+AGG - chr3.4:60427067-60427086 MS.gene008648:CDS 40.0%
AGTGGTCATTTCCATCTTTC+TGG + chr3.4:60427585-60427604 None:intergenic 40.0%
ATCAAAGATCAAGAGGAGGT+TGG - chr3.4:60427734-60427753 MS.gene008648:CDS 40.0%
ATCAAGAGGAGGTTGGAAAT+GGG - chr3.4:60427741-60427760 MS.gene008648:CDS 40.0%
GGAGGATAACATACAGAAAG+AGG - chr3.4:60427088-60427107 MS.gene008648:CDS 40.0%
! TCTTCTTGAGGCAAAGCATT+GGG - chr3.4:60427522-60427541 MS.gene008648:intron 40.0%
!! TAAAAAGATGGTGGATCAGG+AGG - chr3.4:60427499-60427518 MS.gene008648:intron 40.0%
AAGGGAGGATACCAGAAAGA+TGG - chr3.4:60427571-60427590 MS.gene008648:intron 45.0%
ACATGCTTCGACTCCACATA+GGG + chr3.4:60427372-60427391 None:intergenic 45.0%
ACGAGCAGTTGAAGAAGCAA+AGG - chr3.4:60427552-60427571 MS.gene008648:intron 45.0%
AGGTTTGGCCATCAGATTCA+GGG - chr3.4:60427426-60427445 MS.gene008648:CDS 45.0%
CGAGCAGTTGAAGAAGCAAA+GGG - chr3.4:60427553-60427572 MS.gene008648:intron 45.0%
GATCAAGAGGAGGTTGGAAA+TGG - chr3.4:60427740-60427759 MS.gene008648:CDS 45.0%
GGCAATCAAAGATCAAGAGG+AGG - chr3.4:60427730-60427749 MS.gene008648:CDS 45.0%
GTCTTCTTGAGGCAAAGCAT+TGG - chr3.4:60427521-60427540 MS.gene008648:intron 45.0%
TAACATGTGACTCGAAGAGG+AGG - chr3.4:60427070-60427089 MS.gene008648:CDS 45.0%
TCAAGAGGAGGTTGGAAATG+GGG - chr3.4:60427742-60427761 MS.gene008648:CDS 45.0%
TCTAGGTTTAGGAGTTTGCC+TGG - chr3.4:60427464-60427483 MS.gene008648:intron 45.0%
! GGGTCAGAAGTGTGATATCT+AGG - chr3.4:60427447-60427466 MS.gene008648:CDS 45.0%
!!! ATATAGATTTTTTTTTTAAT+AGG - chr3.4:60427317-60427336 MS.gene008648:CDS 5.0%
ACAACCCTCGAGTAGAGGTT+TGG - chr3.4:60427411-60427430 MS.gene008648:CDS 50.0%
ATGGCCAAACCTCTACTCGA+GGG + chr3.4:60427418-60427437 None:intergenic 50.0%
CACATGCTTCGACTCCACAT+AGG + chr3.4:60427373-60427392 None:intergenic 50.0%
CTTCTGACCCCTGAATCTGA+TGG + chr3.4:60427437-60427456 None:intergenic 50.0%
GAGGTTTGGCCATCAGATTC+AGG - chr3.4:60427425-60427444 MS.gene008648:CDS 50.0%
GCAAAACAACCCTCGAGTAG+AGG - chr3.4:60427406-60427425 MS.gene008648:CDS 50.0%
GCAGTTGAAGAAGCAAAGGG+AGG - chr3.4:60427556-60427575 MS.gene008648:intron 50.0%
GGTTTGGCCATCAGATTCAG+GGG - chr3.4:60427427-60427446 MS.gene008648:CDS 50.0%
TGGATCAGGAGGTCTTCTTG+AGG - chr3.4:60427510-60427529 MS.gene008648:intron 50.0%
AGAGGAGGTTGGAAATGGGG+TGG - chr3.4:60427745-60427764 MS.gene008648:CDS 55.0%
GAGGAGGTTGGAAATGGGGT+GGG - chr3.4:60427746-60427765 MS.gene008648:CDS 55.0%
GATGGCCAAACCTCTACTCG+AGG + chr3.4:60427419-60427438 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 60427039 60427775 60427039 ID=MS.gene008648
chr3.4 mRNA 60427039 60427775 60427039 ID=MS.gene008648.t1;Parent=MS.gene008648
chr3.4 exon 60427697 60427775 60427697 ID=MS.gene008648.t1.exon1;Parent=MS.gene008648.t1
chr3.4 CDS 60427697 60427775 60427697 ID=cds.MS.gene008648.t1;Parent=MS.gene008648.t1
chr3.4 exon 60427039 60427475 60427039 ID=MS.gene008648.t1.exon2;Parent=MS.gene008648.t1
chr3.4 CDS 60427039 60427475 60427039 ID=cds.MS.gene008648.t1;Parent=MS.gene008648.t1
Gene Sequence

>MS.gene008648

ATGACTAGAAATAAGGTGAAACTAGCATACATAACATGTGACTCGAAGAGGAGGATAACATACAGAAAGAGGAAAAATGGTTAGATTTAGCCTCTCCATAATTTCAATAAAGCATGTATAGATTATCTTTGCATATCATACACATAGCCTTTTTTCCTAGAAATAACACACATTTTTTTTATATTTTTTAAAACTATAATTTATAGTTTTGAAAATTCGTGATTTAGATTTCATTTAGTAAATTTGAAATTTTACCATCGATTGTATATTGTTCATTGATATAGATTTTTTTTTTAATAGGAATTATGAAGAAGATTAATGAAATTAGCACCCTATGTGGAGTCGAAGCATGTGCCATAATTTCAGAGCAAAACAACCCTCGAGTAGAGGTTTGGCCATCAGATTCAGGGGTCAGAAGTGTGATATCTAGGTTTAGGAGTTTGCCTGGACATGAACAAAGTAAAAAGATGGTGGATCAGGAGGTCTTCTTGAGGCAAAGCATTGGGAAAGTCTACGAGCAGTTGAAGAAGCAAAGGGAGGATACCAGAAAGATGGAAATGACCACTATCATTGATCATTATATTCAAACCATAGAATTTAATGGAAATTCGATGACCAAAGATGATCTTAATGATTTTTCATCTTTTATTGATGAGAATTTGAAGGAGGTTGATCGGAAGATGAAAGAAATGGCAATCAAAGATCAAGAGGAGGTTGGAAATGGGGTGGGATTATGA

Protein sequence

>MS.gene008648.t1

MTRNKVKLAYITCDSKRRITYRKRKNGIMKKINEISTLCGVEACAIISEQNNPRVEVWPSDSGVRSVISRFRSLPGHEQSKKMVDQEVFLRQSIGKVYEQLKKQREDTRKMEMTTIIDHYIQTIEFNGNSMTKDDLNDFSSFIDENLKEVDRKMKEMAIKDQEEVGNGVGL