AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008749

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008749.t1 MTR_4g086835 95.238 420 17 1 1 420 1 417 0.0 823
MS.gene008749.t1 MTR_4g086835 95.000 420 17 2 1 420 1 416 0.0 816
MS.gene008749.t1 MTR_4g113140 39.788 377 158 14 7 367 5 328 2.51e-65 215
MS.gene008749.t1 MTR_1g093080 35.097 359 143 11 44 371 10 309 1.88e-46 164
MS.gene008749.t1 MTR_5g054300 35.942 345 142 12 41 371 16 295 1.88e-44 158
MS.gene008749.t1 MTR_5g017980 58.571 140 51 4 274 410 190 325 1.25e-41 150
MS.gene008749.t1 MTR_5g017980 57.857 140 51 5 274 410 189 323 3.66e-39 143
MS.gene008749.t1 MTR_5g017980 57.857 140 51 5 274 410 190 324 3.67e-39 143
MS.gene008749.t1 MTR_1g093080 34.628 309 120 9 44 327 10 261 8.58e-37 136
MS.gene008749.t1 MTR_1g093080 29.787 282 116 9 44 300 10 234 3.20e-20 89.7
MS.gene008749.t1 MTR_1g053830 47.059 68 36 0 274 341 15 82 4.10e-15 71.2
MS.gene008749.t1 MTR_7g089010 50.746 67 32 1 270 336 84 149 4.19e-15 75.9
MS.gene008749.t1 MTR_2g023580 51.471 68 33 0 263 330 41 108 1.73e-14 73.2
MS.gene008749.t1 MTR_7g069660 40.698 86 51 0 245 330 39 124 4.24e-14 73.6
MS.gene008749.t1 MTR_7g098250 51.471 68 33 0 277 344 262 329 6.94e-14 73.6
MS.gene008749.t1 MTR_1g080330 61.111 54 21 0 277 330 175 228 7.03e-14 72.0
MS.gene008749.t1 MTR_7g115530 62.963 54 20 0 277 330 218 271 7.31e-14 72.4
MS.gene008749.t1 MTR_5g041350 55.172 58 26 0 274 331 12 69 9.65e-14 72.4
MS.gene008749.t1 MTR_7g115530 62.963 54 20 0 277 330 218 271 1.30e-13 72.0
MS.gene008749.t1 MTR_1g080330 61.111 54 21 0 277 330 175 228 1.47e-13 72.0
MS.gene008749.t1 MTR_4g121020 53.731 67 30 1 274 340 212 277 1.53e-13 72.8
MS.gene008749.t1 MTR_4g121020 53.731 67 30 1 274 340 212 277 1.56e-13 72.8
MS.gene008749.t1 MTR_8g066310 48.980 98 43 4 251 341 142 239 1.69e-13 71.6
MS.gene008749.t1 MTR_7g115530 62.963 54 20 0 277 330 218 271 1.73e-13 71.6
MS.gene008749.t1 MTR_4g121020 53.731 67 30 1 274 340 108 173 1.83e-13 72.4
MS.gene008749.t1 MTR_7g088070 49.315 73 36 1 269 341 42 113 1.92e-13 70.9
MS.gene008749.t1 MTR_2g450070 48.052 77 35 3 276 351 212 284 2.04e-13 72.4
MS.gene008749.t1 MTR_6g005650 69.014 71 7 1 232 302 84 139 2.04e-13 70.9
MS.gene008749.t1 MTR_2g450070 48.052 77 35 3 276 351 212 284 2.12e-13 72.4
MS.gene008749.t1 MTR_7g115530 62.963 54 20 0 277 330 218 271 2.34e-13 72.0
MS.gene008749.t1 MTR_1g013170 50.000 68 33 1 272 339 186 252 2.80e-13 71.6
MS.gene008749.t1 MTR_5g014040 47.826 69 35 1 268 336 302 369 2.91e-13 72.0
MS.gene008749.t1 MTR_7g088070 53.968 63 29 0 269 331 42 104 3.60e-13 69.7
MS.gene008749.t1 MTR_4g064730 47.059 68 35 1 273 340 127 193 3.92e-13 70.1
MS.gene008749.t1 MTR_0223s0040 63.265 49 18 0 282 330 23 71 5.24e-13 69.3
MS.gene008749.t1 MTR_4g078810 43.820 89 45 2 273 357 154 241 5.73e-13 70.5
MS.gene008749.t1 MTR_1g112370 61.224 49 19 0 282 330 27 75 9.74e-13 69.3
MS.gene008749.t1 MTR_2g034960 50.746 67 32 1 274 340 208 273 9.91e-13 70.5
MS.gene008749.t1 MTR_3g106220 42.529 87 38 2 276 351 197 282 1.14e-12 70.1
MS.gene008749.t1 MTR_2g027860 60.000 55 22 0 277 331 49 103 1.20e-12 68.9
MS.gene008749.t1 MTR_4g098870 31.250 128 76 2 209 336 273 388 1.23e-12 70.1
MS.gene008749.t1 MTR_4g098870 31.250 128 76 2 209 336 253 368 1.24e-12 70.1
MS.gene008749.t1 MTR_7g405800 84.211 38 6 0 224 261 33 70 1.66e-12 67.0
MS.gene008749.t1 MTR_5g029470 46.465 99 48 2 248 341 187 285 1.69e-12 68.9
MS.gene008749.t1 MTR_4g098870 31.250 128 76 2 209 336 292 407 1.69e-12 69.7
MS.gene008749.t1 MTR_4g098870 31.250 128 76 2 209 336 273 388 1.73e-12 69.7
MS.gene008749.t1 MTR_4g098870 31.250 128 76 2 209 336 253 368 1.77e-12 69.3
MS.gene008749.t1 MTR_2g086450 53.448 58 27 0 274 331 42 99 2.72e-12 68.2
MS.gene008749.t1 MTR_6g032990 51.724 58 28 0 274 331 39 96 3.96e-12 67.8
MS.gene008749.t1 MTR_1g032570 47.761 67 34 1 274 340 128 193 4.29e-12 68.2
MS.gene008749.t1 MTR_7g115530 61.538 52 20 0 277 328 218 269 4.36e-12 66.6
MS.gene008749.t1 MTR_1g032570 47.761 67 34 1 274 340 197 262 4.78e-12 68.2
MS.gene008749.t1 MTR_6g071625 41.935 93 47 2 264 349 184 276 5.66e-12 67.4
MS.gene008749.t1 MTR_1g053835 48.529 68 35 0 274 341 255 322 7.32e-12 67.0
MS.gene008749.t1 MTR_5g027440 54.098 61 26 1 277 335 25 85 1.37e-11 65.1
MS.gene008749.t1 MTR_4g131580 45.205 73 39 1 274 346 197 268 1.67e-11 66.6
MS.gene008749.t1 MTR_7g115530 58.491 53 22 0 277 329 218 270 1.80e-11 65.1
MS.gene008749.t1 MTR_4g081710 51.724 58 28 0 274 331 14 71 1.95e-11 65.5
MS.gene008749.t1 MTR_7g093030 50.877 57 28 0 274 330 209 265 2.85e-11 64.3
MS.gene008749.t1 MTR_4g021790 49.180 61 30 1 275 335 166 225 2.87e-11 64.3
MS.gene008749.t1 MTR_3g450310 50.000 56 28 0 275 330 17 72 3.04e-11 63.2
MS.gene008749.t1 MTR_1g090670 56.364 55 24 0 277 331 44 98 3.06e-11 64.3
MS.gene008749.t1 MTR_6g444980 55.172 58 26 0 274 331 32 89 3.13e-11 65.1
MS.gene008749.t1 MTR_2g084230 45.455 66 36 0 265 330 85 150 3.30e-11 64.3
MS.gene008749.t1 MTR_2g090675 49.180 61 30 1 276 336 160 219 3.35e-11 65.1
MS.gene008749.t1 MTR_6g444980 55.172 58 26 0 274 331 32 89 3.41e-11 64.7
MS.gene008749.t1 MTR_3g064840 49.315 73 36 1 269 340 266 338 3.89e-11 65.1
MS.gene008749.t1 MTR_7g117705 47.826 69 33 2 276 344 190 255 5.16e-11 64.7
MS.gene008749.t1 MTR_2g027800 56.364 55 24 0 277 331 39 93 5.70e-11 63.5
MS.gene008749.t1 MTR_2g084230 45.455 66 36 0 265 330 85 150 5.98e-11 63.9
MS.gene008749.t1 MTR_2g027800 56.364 55 24 0 277 331 39 93 7.06e-11 63.5
MS.gene008749.t1 MTR_7g093030 50.877 57 28 0 274 330 209 265 7.38e-11 63.9
MS.gene008749.t1 MTR_1g021520 41.975 81 45 1 272 352 121 199 8.88e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008749.t1 AT2G03500 40.533 375 158 10 7 368 5 327 1.86e-64 213
MS.gene008749.t1 AT4G37180 35.561 374 157 10 9 377 12 306 7.51e-50 172
MS.gene008749.t1 AT4G37180 35.372 376 162 12 9 377 12 313 7.76e-48 167
MS.gene008749.t1 AT4G37180 35.737 319 135 8 9 327 12 260 3.55e-43 152
MS.gene008749.t1 AT1G49560 53.797 158 57 5 261 406 172 325 1.27e-38 142
MS.gene008749.t1 AT1G68670 69.892 93 20 1 274 358 212 304 7.55e-36 135
MS.gene008749.t1 AT1G68670 35.000 120 62 4 36 139 8 127 1.15e-11 66.2
MS.gene008749.t1 AT1G13300 70.787 89 21 2 276 359 180 268 4.49e-34 130
MS.gene008749.t1 AT1G25550 54.622 119 47 2 274 385 206 324 2.09e-33 128
MS.gene008749.t1 AT1G25550 33.588 131 67 2 36 146 12 142 8.75e-15 75.5
MS.gene008749.t1 AT3G25790 57.292 96 36 1 281 371 200 295 1.70e-30 120
MS.gene008749.t1 AT1G49560 66.667 81 21 2 251 327 164 242 5.11e-25 103
MS.gene008749.t1 AT1G25550 83.333 54 9 0 274 327 206 259 1.10e-24 103
MS.gene008749.t1 AT1G25550 33.588 131 67 2 36 146 12 142 1.55e-14 73.9
MS.gene008749.t1 AT3G25790 78.723 47 10 0 281 327 200 246 1.77e-19 87.8
MS.gene008749.t1 AT3G10760 50.704 71 34 1 266 336 94 163 2.88e-15 77.0
MS.gene008749.t1 AT5G05090 50.746 67 32 1 270 336 74 139 2.90e-15 75.9
MS.gene008749.t1 AT2G40970 50.746 67 32 1 270 336 98 163 3.80e-15 75.1
MS.gene008749.t1 AT2G38300 46.053 76 41 0 255 330 32 107 1.37e-14 75.1
MS.gene008749.t1 AT5G59570 50.000 64 31 1 273 336 137 199 1.78e-14 73.9
MS.gene008749.t1 AT5G59570 50.000 64 31 1 273 336 137 199 1.78e-14 73.9
MS.gene008749.t1 AT3G04450 50.685 73 35 1 258 330 220 291 1.56e-13 71.6
MS.gene008749.t1 AT2G06020 35.652 115 72 1 232 346 41 153 1.98e-13 70.9
MS.gene008749.t1 AT3G04450 43.333 90 50 1 258 347 220 308 3.22e-13 71.6
MS.gene008749.t1 AT3G16857 43.137 102 44 2 276 377 236 323 3.56e-13 72.0
MS.gene008749.t1 AT3G16857 43.137 102 44 2 276 377 166 253 3.60e-13 71.6
MS.gene008749.t1 AT5G58080 50.794 63 30 1 274 336 191 252 3.69e-13 71.6
MS.gene008749.t1 AT3G04450 43.333 90 50 1 258 347 180 268 3.99e-13 70.9
MS.gene008749.t1 AT3G04450 43.333 90 50 1 258 347 180 268 3.99e-13 70.9
MS.gene008749.t1 AT3G46640 48.438 64 32 1 273 336 140 202 4.00e-13 70.5
MS.gene008749.t1 AT3G46640 48.438 64 32 1 273 336 140 202 4.00e-13 70.5
MS.gene008749.t1 AT3G13040 58.929 56 23 0 276 331 240 295 4.00e-13 71.2
MS.gene008749.t1 AT3G13040 58.929 56 23 0 276 331 240 295 4.00e-13 71.2
MS.gene008749.t1 AT3G13040 58.929 56 23 0 276 331 240 295 4.00e-13 71.2
MS.gene008749.t1 AT3G16857 43.137 102 44 2 276 377 236 323 4.17e-13 71.6
MS.gene008749.t1 AT4G28610 52.239 67 31 1 277 342 225 291 5.00e-13 70.9
MS.gene008749.t1 AT2G01760 54.839 62 27 1 275 336 133 193 5.25e-13 70.1
MS.gene008749.t1 AT3G46640 48.438 64 32 1 273 336 140 202 5.46e-13 70.1
MS.gene008749.t1 AT2G01760 54.839 62 27 1 275 336 226 286 6.07e-13 70.5
MS.gene008749.t1 AT2G01760 54.839 62 27 1 275 336 198 258 6.38e-13 70.1
MS.gene008749.t1 AT2G01060 60.000 55 22 0 277 331 15 69 7.64e-13 68.9
MS.gene008749.t1 AT5G29000 56.140 57 25 0 274 330 228 284 1.30e-12 68.6
MS.gene008749.t1 AT5G18240 55.172 58 26 0 274 331 42 99 1.62e-12 68.9
MS.gene008749.t1 AT5G18240 55.172 58 26 0 274 331 42 99 1.62e-12 68.9
MS.gene008749.t1 AT2G20400 50.000 70 34 1 277 345 231 300 1.83e-12 68.9
MS.gene008749.t1 AT2G20400 53.125 64 29 1 277 340 231 293 1.88e-12 68.9
MS.gene008749.t1 AT5G18240 55.172 58 26 0 274 331 42 99 1.93e-12 68.9
MS.gene008749.t1 AT5G18240 55.172 58 26 0 274 331 42 99 2.00e-12 68.9
MS.gene008749.t1 AT5G18240 55.172 58 26 0 274 331 42 99 2.00e-12 68.9
MS.gene008749.t1 AT3G04030 53.448 58 27 0 274 331 42 99 2.34e-12 68.6
MS.gene008749.t1 AT3G04030 53.448 58 27 0 274 331 42 99 2.75e-12 68.6
MS.gene008749.t1 AT5G29000 59.259 54 22 0 277 330 231 284 3.64e-12 68.2
MS.gene008749.t1 AT5G29000 59.259 54 22 0 277 330 188 241 3.71e-12 67.8
MS.gene008749.t1 AT5G29000 59.259 54 22 0 277 330 188 241 3.71e-12 67.8
MS.gene008749.t1 AT2G02060 50.000 62 31 0 269 330 21 82 5.82e-12 65.9
MS.gene008749.t1 AT2G02060 50.000 62 31 0 269 330 21 82 5.82e-12 65.9
MS.gene008749.t1 AT1G14600 51.613 62 30 0 269 330 15 76 6.87e-12 65.9
MS.gene008749.t1 AT5G45580 48.529 68 35 0 277 344 7 74 6.96e-12 65.9
MS.gene008749.t1 AT5G06800 44.156 77 40 2 255 331 173 246 7.05e-12 66.2
MS.gene008749.t1 AT2G25180 46.154 65 34 1 276 340 194 257 7.41e-12 67.8
MS.gene008749.t1 AT5G45580 47.826 69 36 0 277 345 23 91 7.88e-12 65.9
MS.gene008749.t1 AT1G32240 45.455 88 47 1 265 351 200 287 9.22e-12 66.6
MS.gene008749.t1 AT3G12730 55.172 58 26 0 274 331 20 77 9.60e-12 65.1
MS.gene008749.t1 AT1G32240 45.455 88 47 1 265 351 200 287 1.09e-11 66.6
MS.gene008749.t1 AT5G16560 50.000 78 37 2 265 340 206 283 2.44e-11 65.5
MS.gene008749.t1 AT1G67710 51.724 58 28 0 279 336 121 178 2.90e-11 65.5
MS.gene008749.t1 AT1G67710 51.724 58 28 0 279 336 130 187 2.94e-11 65.5
MS.gene008749.t1 AT1G67710 51.724 58 28 0 279 336 195 252 3.58e-11 65.5
MS.gene008749.t1 AT5G06800 44.156 77 40 2 255 331 173 246 4.16e-11 64.7
MS.gene008749.t1 AT1G79430 55.172 58 26 0 274 331 31 88 4.23e-11 64.7
MS.gene008749.t1 AT3G24120 56.364 55 24 0 277 331 41 95 4.46e-11 63.9
MS.gene008749.t1 AT5G06800 44.156 77 40 2 255 331 173 246 4.79e-11 64.3
MS.gene008749.t1 AT3G24120 55.172 58 26 0 274 331 38 95 5.06e-11 63.9
MS.gene008749.t1 AT4G13640 56.364 55 24 0 277 331 37 91 5.08e-11 63.9
MS.gene008749.t1 AT4G13640 56.364 55 24 0 277 331 37 91 5.14e-11 63.5
MS.gene008749.t1 AT2G42660 55.102 49 22 0 282 330 55 103 8.61e-11 62.4
MS.gene008749.t1 AT2G40260 46.154 65 35 0 266 330 71 135 8.98e-11 63.9

Find 92 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAGTTCTTGGAGGTTTA+TGG 0.186325 4.4:+25635478 MS.gene008749:CDS
GGTTCTTCCTTCTCTTCTTT+AGG 0.234160 4.4:-25634189 None:intergenic
AAAGAGAGCCTATTCCTTAC+AGG 0.270823 4.4:-25634210 None:intergenic
TTCAGCCGCAGCAGACTTCT+AGG 0.300158 4.4:+25634358 MS.gene008749:CDS
GAGGAAATTAGCGATGGTTT+TGG 0.303370 4.4:-25633320 None:intergenic
GATCAGTCTGTTGTAGTTCT+TGG 0.310407 4.4:+25635468 MS.gene008749:CDS
ACAGAAGAATACGGTGAAAA+AGG 0.316862 4.4:-25634138 None:intergenic
ACAAATCTCCGGTGCAATTC+CGG 0.337882 4.4:-25634402 None:intergenic
GGAATAAACTCTTCCAAAAC+TGG 0.355348 4.4:-25633707 None:intergenic
ATGGAGTTTGGAGAGTTTAT+CGG 0.366175 4.4:-25633371 None:intergenic
ATGAGTTCTCTAATTTGCTT+TGG 0.366259 4.4:-25635045 None:intergenic
AGAGTTTATCGGAGGTTGTT+TGG 0.367071 4.4:-25633360 None:intergenic
AGAGAGGAAATCATGGAGTT+TGG 0.369125 4.4:-25633383 None:intergenic
TACTACTACAAGATTGAAAA+AGG 0.370440 4.4:-25634089 None:intergenic
GATCGAGAAACAGTTCTAAC+AGG 0.377734 4.4:+25634280 MS.gene008749:CDS
GCAGCAAAGATAAGAAGAAT+TGG 0.382485 4.4:+25633801 MS.gene008749:CDS
AGAGAACTCATGCAAGTTGA+TGG 0.388920 4.4:+25635057 MS.gene008749:CDS
GGATGAGTTCTGTTCAGTTA+TGG 0.403657 4.4:+25633822 MS.gene008749:CDS
CGCATACTCGGCTGCACGGT+TGG 0.404437 4.4:-25634324 None:intergenic
AAGAGTCTGAGGGAAGGATT+TGG 0.407195 4.4:+25634249 MS.gene008749:CDS
AGGAAGAACCTGTAAGGAAT+AGG 0.418786 4.4:+25634202 MS.gene008749:CDS
AATACGAACCGTCGTTTAAG+AGG 0.421808 4.4:-25633468 None:intergenic
CAGTCTGTTGTAGTTCTTGG+AGG 0.429416 4.4:+25635471 MS.gene008749:CDS
AGACGCCGTACTGCTACCTT+TGG 0.431495 4.4:-25635529 None:intergenic
TTCATTTAGCTACAGGATCC+CGG 0.434371 4.4:+25635571 MS.gene008749:CDS
TGAGGGAAGGATTTGGTTCA+AGG 0.440327 4.4:+25634256 MS.gene008749:CDS
ATCGGAGGTTGTTTGGATTG+TGG 0.443195 4.4:-25633353 None:intergenic
TCTTCACTTCATCATTAGTC+AGG 0.447623 4.4:-25635080 None:intergenic
ACATGGAAAAGTTGGTGTAT+AGG 0.458002 4.4:+25635683 MS.gene008749:CDS
GTTAATGCGTTGCAGAAGCT+TGG 0.458197 4.4:+25634423 MS.gene008749:CDS
TCCCTCAGACTCTTCACCTC+AGG 0.461715 4.4:-25634240 None:intergenic
CAGTTGGCGATGTCCCTCCC+CGG 0.465539 4.4:-25635590 None:intergenic
AGTCCACTTCATTTAGCTAC+AGG 0.467143 4.4:+25635564 MS.gene008749:CDS
TCTTGTAGTAGTAATAGAAA+TGG 0.468798 4.4:+25634099 MS.gene008749:CDS
AAAGGTAGCAGTACGGCGTC+TGG 0.473278 4.4:+25635531 MS.gene008749:CDS
TGATCAATCTCTTTCTTCAA+AGG 0.473313 4.4:-25633728 None:intergenic
AAATCTAAACTGAGTTCTGC+AGG 0.491660 4.4:-25633280 None:intergenic
ATTCACAGACATGGAAAAGT+TGG 0.497674 4.4:+25635675 MS.gene008749:CDS
GAAGTCTGCTGCGGCTGAAG+TGG 0.500174 4.4:-25634354 None:intergenic
AAGAGAAGGAAGAACCTGTA+AGG 0.506152 4.4:+25634196 MS.gene008749:CDS
TCATTTAGCTACAGGATCCC+GGG 0.515125 4.4:+25635572 MS.gene008749:CDS
ATCAGCACCTAAAGAAGAGA+AGG 0.517481 4.4:+25634182 MS.gene008749:CDS
TGTAGTAGTAATAGAAATGG+AGG 0.522008 4.4:+25634102 MS.gene008749:CDS
CAGAAGCTTGGAGGTTCTCA+AGG 0.522479 4.4:+25634435 MS.gene008749:CDS
CTAGGAAACAGAGGAGATGC+TGG 0.524226 4.4:+25634376 MS.gene008749:CDS
CGAGTCGAGAGAGGAAATCA+TGG 0.525149 4.4:-25633390 None:intergenic
CTCCTGAGGTGAAGAGTCTG+AGG 0.527871 4.4:+25634238 MS.gene008749:CDS
GAGTCGTTGTATTGTTCTTG+AGG 0.536314 4.4:-25635504 None:intergenic
TGATCAGTACCACTTGCAGC+TGG 0.538762 4.4:-25635450 None:intergenic
ATACTCGGCTGCACGGTTGG+AGG 0.545035 4.4:-25634321 None:intergenic
AGTTGGCGATGTCCCTCCCC+GGG 0.547122 4.4:-25635589 None:intergenic
TGACAGAAAAGGGAGGAAGA+TGG 0.549726 4.4:+25634042 MS.gene008749:intron
GCTAAATGAAGTGGACTTTG+TGG 0.551266 4.4:-25635558 None:intergenic
AATGCGTTGCAGAAGCTTGG+AGG 0.554542 4.4:+25634426 MS.gene008749:CDS
ACAGAGGAGATGCTGGTCAC+CGG 0.560535 4.4:+25634383 MS.gene008749:CDS
GAACTCTCCGCGTATGAAGT+CGG 0.562568 4.4:-25635428 None:intergenic
GCTGGTCACCGGAATTGCAC+CGG 0.565693 4.4:+25634394 MS.gene008749:CDS
TAGCTACAGGATCCCGGGGA+GGG 0.570717 4.4:+25635577 MS.gene008749:CDS
GCAACGCATTAACAAATCTC+CGG 0.573053 4.4:-25634413 None:intergenic
AGTGGCGCAGCACGCATACT+CGG 0.578170 4.4:-25634336 None:intergenic
GGAGGGACATCGCCAACTGA+AGG 0.584248 4.4:+25635594 MS.gene008749:CDS
GTCACAGGAACAGAAGAATA+CGG 0.587549 4.4:-25634147 None:intergenic
TCCATGCTCCTCTTAAACGA+CGG 0.593486 4.4:+25633460 MS.gene008749:CDS
GCAGGAACAACACAACCCAT+TGG 0.594618 4.4:-25633262 None:intergenic
GCCAACTGAAGGTGATAGCA+TGG 0.596672 4.4:+25635605 MS.gene008749:CDS
ATGATAGAGGAAATTAGCGA+TGG 0.597212 4.4:-25633326 None:intergenic
TCTTAATAAATTAGAAACAA+AGG 0.606924 4.4:+25633910 MS.gene008749:CDS
GATCCTGTAGCTAAATGAAG+TGG 0.608872 4.4:-25635567 None:intergenic
TCCATGCTATCACCTTCAGT+TGG 0.614177 4.4:-25635606 None:intergenic
AAAAGTCATATTCACAGACA+TGG 0.616407 4.4:+25635666 MS.gene008749:CDS
GATAATGTCACTGTTGTCAC+AGG 0.616977 4.4:-25634162 None:intergenic
AGGGAAGGATTTGGTTCAAG+GGG 0.617751 4.4:+25634258 MS.gene008749:CDS
AGCACGCATACTCGGCTGCA+CGG 0.618042 4.4:-25634328 None:intergenic
GTTCATCTTCGAGTCGAGAG+AGG 0.619371 4.4:-25633399 None:intergenic
GGATTGTGGAAAGATGATAG+AGG 0.619765 4.4:-25633339 None:intergenic
CAATACAACGACTCTTCCAA+AGG 0.620750 4.4:+25635513 MS.gene008749:CDS
GAGGGAAGGATTTGGTTCAA+GGG 0.625225 4.4:+25634257 MS.gene008749:CDS
TCCTGAGGTGAAGAGTCTGA+GGG 0.627907 4.4:+25634239 MS.gene008749:CDS
GAGGTGAAGAGTCTGAGGGA+AGG 0.631423 4.4:+25634243 MS.gene008749:CDS
GAGTTTGGAGAGTTTATCGG+AGG 0.638169 4.4:-25633368 None:intergenic
CGGAGAGTTCCAGCTGCAAG+TGG 0.640628 4.4:+25635441 MS.gene008749:CDS
TTTAAGAGGAGCATGGAGAG+AGG 0.641505 4.4:-25633454 None:intergenic
CTCTTCCAAAGGTAGCAGTA+CGG 0.648767 4.4:+25635524 MS.gene008749:CDS
AGCAGACTTCTAGGAAACAG+AGG 0.657415 4.4:+25634367 MS.gene008749:CDS
ACAATTTGTGACTGCAGCTG+AGG 0.658528 4.4:+25634065 MS.gene008749:CDS
CATTTAGCTACAGGATCCCG+GGG 0.660202 4.4:+25635573 MS.gene008749:CDS
ACCGTCGTTTAAGAGGAGCA+TGG 0.663203 4.4:-25633461 None:intergenic
AGAAATACCGACTTCATACG+CGG 0.668200 4.4:+25635421 MS.gene008749:intron
ATCGAGAAACAGTTCTAACA+GGG 0.670598 4.4:+25634281 MS.gene008749:CDS
TGTTTCCTAGAAGTCTGCTG+CGG 0.688682 4.4:-25634363 None:intergenic
TTAGCTACAGGATCCCGGGG+AGG 0.690072 4.4:+25635576 MS.gene008749:CDS
TTGGTGATGACAGAAAAGGG+AGG 0.712344 4.4:+25634035 MS.gene008749:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCTATAATATTTCCAAAT+TGG - chr4.4:25634580-25634599 None:intergenic 15.0%
!! TCTTAATAAATTAGAAACAA+AGG + chr4.4:25633910-25633929 MS.gene008749:CDS 15.0%
!!! TTTTTTCTTGTCTAATTTTT+AGG - chr4.4:25633979-25633998 None:intergenic 15.0%
!! ATTATAGATTAGTGAATTGA+TGG + chr4.4:25634590-25634609 MS.gene008749:intron 20.0%
!! TAATAACATTACATCTTGTT+TGG + chr4.4:25634982-25635001 MS.gene008749:intron 20.0%
!! TTGAATAAAGTTATCAACTT+TGG + chr4.4:25633572-25633591 MS.gene008749:intron 20.0%
! ACCCACATAAAAAATTCAAA+AGG - chr4.4:25633551-25633570 None:intergenic 25.0%
! ATATAGACAAGTCAATTTGT+AGG + chr4.4:25634774-25634793 MS.gene008749:intron 25.0%
! CCCACATAAAAAATTCAAAA+GGG - chr4.4:25633550-25633569 None:intergenic 25.0%
! CTTTATTAACCTTAGTTACT+AGG + chr4.4:25635159-25635178 MS.gene008749:intron 25.0%
! GTTAAGACCAAAAAACATAT+AGG + chr4.4:25634901-25634920 MS.gene008749:intron 25.0%
! TACTACTACAAGATTGAAAA+AGG - chr4.4:25634092-25634111 None:intergenic 25.0%
! TCTTGTAGTAGTAATAGAAA+TGG + chr4.4:25634099-25634118 MS.gene008749:CDS 25.0%
! TGTTTAAATTTAGATCAGCA+AGG + chr4.4:25634732-25634751 MS.gene008749:intron 25.0%
! TTGAGTTATCAAGTTTGAAT+TGG + chr4.4:25635366-25635385 MS.gene008749:intron 25.0%
!! ACCCTTTTGAATTTTTTATG+TGG + chr4.4:25633546-25633565 MS.gene008749:intron 25.0%
!! ATGATGATGATGATATTGAA+AGG - chr4.4:25633495-25633514 None:intergenic 25.0%
!! CCCTTTTGAATTTTTTATGT+GGG + chr4.4:25633547-25633566 MS.gene008749:intron 25.0%
!! TGATGATGATGATATTGAAA+GGG - chr4.4:25633494-25633513 None:intergenic 25.0%
!!! AAACTTTACTGTTTTTGTGA+TGG + chr4.4:25634468-25634487 MS.gene008749:intron 25.0%
!!! ATGCTAGTCTTAAAAGTTTA+AGG + chr4.4:25634628-25634647 MS.gene008749:intron 25.0%
!!! TTTGGAGTTTTTGATTCAAT+GGG + chr4.4:25633590-25633609 MS.gene008749:intron 25.0%
AAAAATCTGAGAGTTATAGC+TGG + chr4.4:25635643-25635662 MS.gene008749:CDS 30.0%
AAAAGTCATATTCACAGACA+TGG + chr4.4:25635666-25635685 MS.gene008749:CDS 30.0%
AATTGCAAAGGCAAATCATT+TGG - chr4.4:25634937-25634956 None:intergenic 30.0%
ACCAAAAACTTACTTGCAAA+TGG - chr4.4:25635107-25635126 None:intergenic 30.0%
TAATAAAGCATGCAGTTAAG+AGG - chr4.4:25635147-25635166 None:intergenic 30.0%
TCATATTGTATGTGCATTCA+TGG + chr4.4:25635308-25635327 MS.gene008749:intron 30.0%
TGATAATCCATAGTTTGGTT+GGG - chr4.4:25634854-25634873 None:intergenic 30.0%
TGATCAATCTCTTTCTTCAA+AGG - chr4.4:25633731-25633750 None:intergenic 30.0%
TGCAACAGTAATCTTAATGA+GGG + chr4.4:25634000-25634019 MS.gene008749:intron 30.0%
TGTAGTAGTAATAGAAATGG+AGG + chr4.4:25634102-25634121 MS.gene008749:CDS 30.0%
TTGCAACAGTAATCTTAATG+AGG + chr4.4:25633999-25634018 MS.gene008749:intron 30.0%
! CAATTTTGCATACGTAGTTT+TGG + chr4.4:25634952-25634971 MS.gene008749:intron 30.0%
! TAAAAATTCTGTGCCAGTTT+TGG + chr4.4:25633694-25633713 MS.gene008749:CDS 30.0%
! TTTTGTGTATGCAGCAATTT+TGG + chr4.4:25633640-25633659 MS.gene008749:intron 30.0%
!! ACACTCAATTTTGACCAATT+TGG + chr4.4:25634563-25634582 MS.gene008749:intron 30.0%
!! ATGAGTTCTCTAATTTGCTT+TGG - chr4.4:25635048-25635067 None:intergenic 30.0%
!! TTGGAGTTTTTGATTCAATG+GGG + chr4.4:25633591-25633610 MS.gene008749:intron 30.0%
!!! ATGAGGGTTTTGTTTTTGTT+TGG + chr4.4:25634016-25634035 MS.gene008749:intron 30.0%
!!! CTTTGGAGTTTTTGATTCAA+TGG + chr4.4:25633589-25633608 MS.gene008749:intron 30.0%
!!! TGATAGGCCTATATGTTTTT+TGG - chr4.4:25634911-25634930 None:intergenic 30.0%
AAATCTAAACTGAGTTCTGC+AGG - chr4.4:25633283-25633302 None:intergenic 35.0%
AATCTGATCAAGGATTGTTC+CGG - chr4.4:25634823-25634842 None:intergenic 35.0%
ACAGAAGAATACGGTGAAAA+AGG - chr4.4:25634141-25634160 None:intergenic 35.0%
AGTTACTAGGTGTTTGATGA+TGG + chr4.4:25635172-25635191 MS.gene008749:intron 35.0%
ATGATAGAGGAAATTAGCGA+TGG - chr4.4:25633329-25633348 None:intergenic 35.0%
ATGGAGTTTGGAGAGTTTAT+CGG - chr4.4:25633374-25633393 None:intergenic 35.0%
ATTCACAGACATGGAAAAGT+TGG + chr4.4:25635675-25635694 MS.gene008749:CDS 35.0%
CATCAAACACCTAGTAACTA+AGG - chr4.4:25635171-25635190 None:intergenic 35.0%
CTACGTATGCAAAATTGCAA+AGG - chr4.4:25634949-25634968 None:intergenic 35.0%
CTGATAATCCATAGTTTGGT+TGG - chr4.4:25634855-25634874 None:intergenic 35.0%
GCAAATCATTTGGACTTGAT+AGG - chr4.4:25634927-25634946 None:intergenic 35.0%
GCAGCAAAGATAAGAAGAAT+TGG + chr4.4:25633801-25633820 MS.gene008749:CDS 35.0%
GGAATAAACTCTTCCAAAAC+TGG - chr4.4:25633710-25633729 None:intergenic 35.0%
GGAGGTTATTAATCTGATCA+AGG - chr4.4:25634833-25634852 None:intergenic 35.0%
TAAACAATCCCTAACTTCAC+TGG - chr4.4:25634718-25634737 None:intergenic 35.0%
TCTATCCCGAATTCAAATCT+TGG + chr4.4:25634797-25634816 MS.gene008749:intron 35.0%
TCTTATGTAGTTGTGTTGTG+AGG + chr4.4:25634533-25634552 MS.gene008749:intron 35.0%
TCTTCACTTCATCATTAGTC+AGG - chr4.4:25635083-25635102 None:intergenic 35.0%
TTAGTTCACCAGTGAAGTTA+GGG + chr4.4:25634707-25634726 MS.gene008749:intron 35.0%
TTGTAGTTCTTGGAGGTTTA+TGG + chr4.4:25635478-25635497 MS.gene008749:CDS 35.0%
TTTGATTCAATGGGGCTAAT+GGG + chr4.4:25633599-25633618 MS.gene008749:intron 35.0%
! AGGAAATTAGCGATGGTTTT+GGG - chr4.4:25633322-25633341 None:intergenic 35.0%
! AGGCATTTAGGATTGAGATT+TGG + chr4.4:25635343-25635362 MS.gene008749:intron 35.0%
! ATCGAGAAACAGTTCTAACA+GGG + chr4.4:25634281-25634300 MS.gene008749:CDS 35.0%
! GCCATTTGCAAGTAAGTTTT+TGG + chr4.4:25635103-25635122 MS.gene008749:intron 35.0%
! TTTAGTTCACCAGTGAAGTT+AGG + chr4.4:25634706-25634725 MS.gene008749:intron 35.0%
!! TGTTTGGTGATGACAGAAAA+GGG + chr4.4:25634032-25634051 MS.gene008749:intron 35.0%
!! TTGTTTGGTGATGACAGAAA+AGG + chr4.4:25634031-25634050 MS.gene008749:intron 35.0%
!! TTTTGATTCAATGGGGCTAA+TGG + chr4.4:25633598-25633617 MS.gene008749:intron 35.0%
!!! AGCAATTTTGGTTTTGAAGG+AGG + chr4.4:25633652-25633671 MS.gene008749:intron 35.0%
!!! GTTTTTTGGTCTTAACCTTC+CGG - chr4.4:25634897-25634916 None:intergenic 35.0%
!!! TGCAGCAATTTTGGTTTTGA+AGG + chr4.4:25633649-25633668 MS.gene008749:intron 35.0%
!!! TTTGGTTTTGAAGGAGGAAT+TGG + chr4.4:25633658-25633677 MS.gene008749:CDS 35.0%
AAAGAGAGCCTATTCCTTAC+AGG - chr4.4:25634213-25634232 None:intergenic 40.0%
AAGAGAAGGAAGAACCTGTA+AGG + chr4.4:25634196-25634215 MS.gene008749:CDS 40.0%
AATACGAACCGTCGTTTAAG+AGG - chr4.4:25633471-25633490 None:intergenic 40.0%
AGAGAGGAAATCATGGAGTT+TGG - chr4.4:25633386-25633405 None:intergenic 40.0%
AGAGTTTATCGGAGGTTGTT+TGG - chr4.4:25633363-25633382 None:intergenic 40.0%
AGATCAGCAAGGAGACAAAT+AGG + chr4.4:25634743-25634762 MS.gene008749:intron 40.0%
AGGAAGAACCTGTAAGGAAT+AGG + chr4.4:25634202-25634221 MS.gene008749:CDS 40.0%
AGTCCACTTCATTTAGCTAC+AGG + chr4.4:25635564-25635583 MS.gene008749:CDS 40.0%
ATAACCTCCCAACCAAACTA+TGG + chr4.4:25634844-25634863 MS.gene008749:intron 40.0%
ATCAGCACCTAAAGAAGAGA+AGG + chr4.4:25634182-25634201 MS.gene008749:CDS 40.0%
ATTCATGGTGTCTGGTTATG+AGG + chr4.4:25635323-25635342 MS.gene008749:intron 40.0%
CAATACAACGACTCTTCCAA+AGG + chr4.4:25635513-25635532 MS.gene008749:CDS 40.0%
GAGTCGTTGTATTGTTCTTG+AGG - chr4.4:25635507-25635526 None:intergenic 40.0%
GATAATGTCACTGTTGTCAC+AGG - chr4.4:25634165-25634184 None:intergenic 40.0%
GATCAGTCTGTTGTAGTTCT+TGG + chr4.4:25635468-25635487 MS.gene008749:CDS 40.0%
GATCCTGTAGCTAAATGAAG+TGG - chr4.4:25635570-25635589 None:intergenic 40.0%
GCAACGCATTAACAAATCTC+CGG - chr4.4:25634416-25634435 None:intergenic 40.0%
GGATGAGTTCTGTTCAGTTA+TGG + chr4.4:25633822-25633841 MS.gene008749:CDS 40.0%
GGATTGTGGAAAGATGATAG+AGG - chr4.4:25633342-25633361 None:intergenic 40.0%
GGGACTGATAATCCATAGTT+TGG - chr4.4:25634859-25634878 None:intergenic 40.0%
GGTTCTTCCTTCTCTTCTTT+AGG - chr4.4:25634192-25634211 None:intergenic 40.0%
GTCACAGGAACAGAAGAATA+CGG - chr4.4:25634150-25634169 None:intergenic 40.0%
TAATCCATAGTTTGGTTGGG+AGG - chr4.4:25634851-25634870 None:intergenic 40.0%
TTCATTTAGCTACAGGATCC+CGG + chr4.4:25635571-25635590 MS.gene008749:CDS 40.0%
TTCCGGCCAAGATTTGAATT+CGG - chr4.4:25634806-25634825 None:intergenic 40.0%
! AGAGAACTCATGCAAGTTGA+TGG + chr4.4:25635057-25635076 MS.gene008749:CDS 40.0%
! GAGGAAATTAGCGATGGTTT+TGG - chr4.4:25633323-25633342 None:intergenic 40.0%
! GATCGAGAAACAGTTCTAAC+AGG + chr4.4:25634280-25634299 MS.gene008749:CDS 40.0%
! GCTAAATGAAGTGGACTTTG+TGG - chr4.4:25635561-25635580 None:intergenic 40.0%
! TAGTGAATTGATGGATGTGC+TGG + chr4.4:25634599-25634618 MS.gene008749:intron 40.0%
! TGTCTGGTTATGAGGCATTT+AGG + chr4.4:25635331-25635350 MS.gene008749:intron 40.0%
!! AGAAATACCGACTTCATACG+CGG + chr4.4:25635421-25635440 MS.gene008749:intron 40.0%
!!! ATGGTTTTGGGAACGAAACT+TGG - chr4.4:25633310-25633329 None:intergenic 40.0%
AAGAGTCTGAGGGAAGGATT+TGG + chr4.4:25634249-25634268 MS.gene008749:CDS 45.0%
ACAAATCTCCGGTGCAATTC+CGG - chr4.4:25634405-25634424 None:intergenic 45.0%
ACAATTTGTGACTGCAGCTG+AGG + chr4.4:25634065-25634084 MS.gene008749:CDS 45.0%
ATCGGAGGTTGTTTGGATTG+TGG - chr4.4:25633356-25633375 None:intergenic 45.0%
CAGTCTGTTGTAGTTCTTGG+AGG + chr4.4:25635471-25635490 MS.gene008749:CDS 45.0%
CTCTTCCAAAGGTAGCAGTA+CGG + chr4.4:25635524-25635543 MS.gene008749:CDS 45.0%
GAGTTTGGAGAGTTTATCGG+AGG - chr4.4:25633371-25633390 None:intergenic 45.0%
GTATGTGCATTCATGGTGTC+TGG + chr4.4:25635315-25635334 MS.gene008749:intron 45.0%
GTTAATGCGTTGCAGAAGCT+TGG + chr4.4:25634423-25634442 MS.gene008749:CDS 45.0%
TCATTTAGCTACAGGATCCC+GGG + chr4.4:25635572-25635591 MS.gene008749:CDS 45.0%
TCCATGCTATCACCTTCAGT+TGG - chr4.4:25635609-25635628 None:intergenic 45.0%
TCCATGCTCCTCTTAAACGA+CGG + chr4.4:25633460-25633479 MS.gene008749:CDS 45.0%
TCCCGAATTCAAATCTTGGC+CGG + chr4.4:25634801-25634820 MS.gene008749:intron 45.0%
TCCGGCCAAGATTTGAATTC+GGG - chr4.4:25634805-25634824 None:intergenic 45.0%
TCTTAACCTTCCGGTTCCTA+GGG - chr4.4:25634888-25634907 None:intergenic 45.0%
TGACAGAAAAGGGAGGAAGA+TGG + chr4.4:25634042-25634061 MS.gene008749:intron 45.0%
TGTTTCCTAGAAGTCTGCTG+CGG - chr4.4:25634366-25634385 None:intergenic 45.0%
! AGCAGACTTCTAGGAAACAG+AGG + chr4.4:25634367-25634386 MS.gene008749:CDS 45.0%
! AGGGAAGGATTTGGTTCAAG+GGG + chr4.4:25634258-25634277 MS.gene008749:CDS 45.0%
! GAGGGAAGGATTTGGTTCAA+GGG + chr4.4:25634257-25634276 MS.gene008749:CDS 45.0%
! TGAGGGAAGGATTTGGTTCA+AGG + chr4.4:25634256-25634275 MS.gene008749:CDS 45.0%
! TTTAAGAGGAGCATGGAGAG+AGG - chr4.4:25633457-25633476 None:intergenic 45.0%
!! TTGGTGATGACAGAAAAGGG+AGG + chr4.4:25634035-25634054 MS.gene008749:intron 45.0%
AATGCGTTGCAGAAGCTTGG+AGG + chr4.4:25634426-25634445 MS.gene008749:CDS 50.0%
ACCGTCGTTTAAGAGGAGCA+TGG - chr4.4:25633464-25633483 None:intergenic 50.0%
CAGAAGCTTGGAGGTTCTCA+AGG + chr4.4:25634435-25634454 MS.gene008749:CDS 50.0%
CATTTAGCTACAGGATCCCG+GGG + chr4.4:25635573-25635592 MS.gene008749:CDS 50.0%
CGAGTCGAGAGAGGAAATCA+TGG - chr4.4:25633393-25633412 None:intergenic 50.0%
CTAGGAAACAGAGGAGATGC+TGG + chr4.4:25634376-25634395 MS.gene008749:CDS 50.0%
GAACTCTCCGCGTATGAAGT+CGG - chr4.4:25635431-25635450 None:intergenic 50.0%
GCCAACTGAAGGTGATAGCA+TGG + chr4.4:25635605-25635624 MS.gene008749:CDS 50.0%
GTCTTAACCTTCCGGTTCCT+AGG - chr4.4:25634889-25634908 None:intergenic 50.0%
GTTCATCTTCGAGTCGAGAG+AGG - chr4.4:25633402-25633421 None:intergenic 50.0%
TATCAGTCCCTCCTTTCCCT+AGG + chr4.4:25634869-25634888 MS.gene008749:intron 50.0%
TCCTGAGGTGAAGAGTCTGA+GGG + chr4.4:25634239-25634258 MS.gene008749:CDS 50.0%
TGATCAGTACCACTTGCAGC+TGG - chr4.4:25635453-25635472 None:intergenic 50.0%
! GCTCTCTTTTCTGACTCCTG+AGG + chr4.4:25634224-25634243 MS.gene008749:CDS 50.0%
AAAGGTAGCAGTACGGCGTC+TGG + chr4.4:25635531-25635550 MS.gene008749:CDS 55.0%
ACAGAGGAGATGCTGGTCAC+CGG + chr4.4:25634383-25634402 MS.gene008749:CDS 55.0%
ACCTTCCGGTTCCTAGGGAA+AGG - chr4.4:25634883-25634902 None:intergenic 55.0%
AGACGCCGTACTGCTACCTT+TGG - chr4.4:25635532-25635551 None:intergenic 55.0%
CTCCTGAGGTGAAGAGTCTG+AGG + chr4.4:25634238-25634257 MS.gene008749:CDS 55.0%
GAGGTGAAGAGTCTGAGGGA+AGG + chr4.4:25634243-25634262 MS.gene008749:CDS 55.0%
TCCCTCAGACTCTTCACCTC+AGG - chr4.4:25634243-25634262 None:intergenic 55.0%
TCCCTCCTTTCCCTAGGAAC+CGG + chr4.4:25634875-25634894 MS.gene008749:intron 55.0%
TCCGGTTCCTAGGGAAAGGA+GGG - chr4.4:25634879-25634898 None:intergenic 55.0%
TCCTTTCCCTAGGAACCGGA+AGG + chr4.4:25634879-25634898 MS.gene008749:intron 55.0%
TTCAGCCGCAGCAGACTTCT+AGG + chr4.4:25634358-25634377 MS.gene008749:CDS 55.0%
TTCCGGTTCCTAGGGAAAGG+AGG - chr4.4:25634880-25634899 None:intergenic 55.0%
AGCACGCATACTCGGCTGCA+CGG - chr4.4:25634331-25634350 None:intergenic 60.0%
AGTGGCGCAGCACGCATACT+CGG - chr4.4:25634339-25634358 None:intergenic 60.0%
ATACTCGGCTGCACGGTTGG+AGG - chr4.4:25634324-25634343 None:intergenic 60.0%
CGGAGAGTTCCAGCTGCAAG+TGG + chr4.4:25635441-25635460 MS.gene008749:CDS 60.0%
GAAGTCTGCTGCGGCTGAAG+TGG - chr4.4:25634357-25634376 None:intergenic 60.0%
GCTGGTCACCGGAATTGCAC+CGG + chr4.4:25634394-25634413 MS.gene008749:CDS 60.0%
GGAGGGACATCGCCAACTGA+AGG + chr4.4:25635594-25635613 MS.gene008749:CDS 60.0%
TAGCTACAGGATCCCGGGGA+GGG + chr4.4:25635577-25635596 MS.gene008749:CDS 60.0%
TTAGCTACAGGATCCCGGGG+AGG + chr4.4:25635576-25635595 MS.gene008749:CDS 60.0%
AGTTGGCGATGTCCCTCCCC+GGG - chr4.4:25635592-25635611 None:intergenic 65.0%
CAGTTGGCGATGTCCCTCCC+CGG - chr4.4:25635593-25635612 None:intergenic 65.0%
CGCATACTCGGCTGCACGGT+TGG - chr4.4:25634327-25634346 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 25633265 25635704 25633265 ID=MS.gene008749
chr4.4 mRNA 25633265 25635704 25633265 ID=MS.gene008749.t1;Parent=MS.gene008749
chr4.4 exon 25633265 25633481 25633265 ID=MS.gene008749.t1.exon1;Parent=MS.gene008749.t1
chr4.4 CDS 25633265 25633481 25633265 ID=cds.MS.gene008749.t1;Parent=MS.gene008749.t1
chr4.4 exon 25633654 25633931 25633654 ID=MS.gene008749.t1.exon2;Parent=MS.gene008749.t1
chr4.4 CDS 25633654 25633931 25633654 ID=cds.MS.gene008749.t1;Parent=MS.gene008749.t1
chr4.4 exon 25634048 25634456 25634048 ID=MS.gene008749.t1.exon3;Parent=MS.gene008749.t1
chr4.4 CDS 25634048 25634456 25634048 ID=cds.MS.gene008749.t1;Parent=MS.gene008749.t1
chr4.4 exon 25635037 25635113 25635037 ID=MS.gene008749.t1.exon4;Parent=MS.gene008749.t1
chr4.4 CDS 25635037 25635113 25635037 ID=cds.MS.gene008749.t1;Parent=MS.gene008749.t1
chr4.4 exon 25635423 25635704 25635423 ID=MS.gene008749.t1.exon5;Parent=MS.gene008749.t1
chr4.4 CDS 25635423 25635704 25635423 ID=cds.MS.gene008749.t1;Parent=MS.gene008749.t1
Gene Sequence

>MS.gene008749

ATGGGTTGTGTTGTTCCTGCAGAACTCAGTTTAGATTTAAGACCAAGTTTCGTTCCCAAAACCATCGCTAATTTCCTCTATCATCTTTCCACAATCCAAACAACCTCCGATAAACTCTCCAAACTCCATGATTTCCTCTCTCGACTCGAAGATGAACTCAATAAGATCGATGCTTTCAAACGTGAACTACCTCTCTCCATGCTCCTCTTAAACGACGGTTCGTATTCCCTTTCAATATCATCATCATCATCATAATCTTCGTTTAATTCAATTTTTATGATACCCTTTTGAATTTTTTATGTGGGTTTTGAATAAAGTTATCAACTTTGGAGTTTTTGATTCAATGGGGCTAATGGGTTTGTTTTGTTTTTGTGTTTTTGTGTATGCAGCAATTTTGGTTTTGAAGGAGGAATTGGAAAAATGTAGAAGTAAAAATTCTGTGCCAGTTTTGGAAGAGTTTATTCCTTTGAAGAAAGAGATTGATCAGAGTGAAGAGAATAATAATAATGATAGAGACAAGAACAACAACAATGAATGCAGCAAAGATAAGAAGAATTGGATGAGTTCTGTTCAGTTATGGAATAACAACACAACAACTTCTAATAATAATGTCTCTGATCATCATCATAAACATAACAATCATAATCTTAATAAATTAGAAACAAAGGTACTAAAAAAAATTAACAATATTGTTTATTCATTTAGATTAATCCTAAAAATTAGACAAGAAAAAATTGCAACAGTAATCTTAATGAGGGTTTTGTTTTTGTTTGGTGATGACAGAAAAGGGAGGAAGATGGACAATTTGTGACTGCAGCTGAGGACCTTTTTCAATCTTGTAGTAGTAATAGAAATGGAGGAAGTGCTTTTATGCCTTTTTCACCGTATTCTTCTGTTCCTGTGACAACAGTGACATTATCAGCACCTAAAGAAGAGAAGGAAGAACCTGTAAGGAATAGGCTCTCTTTTCTGACTCCTGAGGTGAAGAGTCTGAGGGAAGGATTTGGTTCAAGGGGATCGAGAAACAGTTCTAACAGGGCAGTTTCGTCGTCGTCTCCTCCAACCGTGCAGCCGAGTATGCGTGCTGCGCCACTTCAGCCGCAGCAGACTTCTAGGAAACAGAGGAGATGCTGGTCACCGGAATTGCACCGGAGATTTGTTAATGCGTTGCAGAAGCTTGGAGGTTCTCAAGGTATGAATTTGAAACTTTACTGTTTTTGTGATGGAGCATATATGTTATTAAATTAGAAATGTACATATATTTAATATCTTATGTAGTTGTGTTGTGAGGTTTCAAAACACTCAATTTTGACCAATTTGGAAATATTATAGATTAGTGAATTGATGGATGTGCTGGAACTGAATGCTAGTCTTAAAAGTTTAAGGATTTGATGCTTGAAGTTGTGTTGTTGTGAAATTTTAAAATAATATGTTTGTTCTGTTTAGTTCACCAGTGAAGTTAGGGATTGTTTAAATTTAGATCAGCAAGGAGACAAATAGGAGTGTTATATATAGACAAGTCAATTTGTAGGTCTATCCCGAATTCAAATCTTGGCCGGAACAATCCTTGATCAGATTAATAACCTCCCAACCAAACTATGGATTATCAGTCCCTCCTTTCCCTAGGAACCGGAAGGTTAAGACCAAAAAACATATAGGCCTATCAAGTCCAAATGATTTGCCTTTGCAATTTTGCATACGTAGTTTTGGTATGCTGTAATAACATTACATCTTGTTTGGTTCATTTGTCTAATTAACTGTTTTAACTGCAGCAGCAACTCCAAAGCAAATTAGAGAACTCATGCAAGTTGATGGCCTGACTAATGATGAAGTGAAGAGCCATTTGCAAGTAAGTTTTTGGTATTCTTTTAATTTTTCTCCTCTTAACTGCATGCTTTATTAACCTTAGTTACTAGGTGTTTGATGATGGTTATGATATAGTTAATTTAATACAGTATTAATTATATTATGTTGCTATTGAAGTATCGTCAATCACAGATATAAACGATGTATACTAGATGATATTTATGATGATGATGAGTATCATATTGTATGTGCATTCATGGTGTCTGGTTATGAGGCATTTAGGATTGAGATTTGGTTGAGTTATCAAGTTTGAATTGGATGTTTGACATATGATTCTTGATTATTATTGCAGAAATACCGACTTCATACGCGGAGAGTTCCAGCTGCAAGTGGTACTGATCAGTCTGTTGTAGTTCTTGGAGGTTTATGGATGCCTCAAGAACAATACAACGACTCTTCCAAAGGTAGCAGTACGGCGTCTGGTTCTCCACAAAGTCCACTTCATTTAGCTACAGGATCCCGGGGAGGGACATCGCCAACTGAAGGTGATAGCATGGACGATGACGATGATGAAAAATCTGAGAGTTATAGCTGGAAAAGTCATATTCACAGACATGGAAAAGTTGGTGTATAG
Protein sequence

>MS.gene008749.t1

MGCVVPAELSLDLRPSFVPKTIANFLYHLSTIQTTSDKLSKLHDFLSRLEDELNKIDAFKRELPLSMLLLNDAILVLKEELEKCRSKNSVPVLEEFIPLKKEIDQSEENNNNDRDKNNNNECSKDKKNWMSSVQLWNNNTTTSNNNVSDHHHKHNNHNLNKLETKKREEDGQFVTAAEDLFQSCSSNRNGGSAFMPFSPYSSVPVTTVTLSAPKEEKEEPVRNRLSFLTPEVKSLREGFGSRGSRNSSNRAVSSSSPPTVQPSMRAAPLQPQQTSRKQRRCWSPELHRRFVNALQKLGGSQAATPKQIRELMQVDGLTNDEVKSHLQKYRLHTRRVPAASGTDQSVVVLGGLWMPQEQYNDSSKGSSTASGSPQSPLHLATGSRGGTSPTEGDSMDDDDDEKSESYSWKSHIHRHGKVGV