AlfalfaGEDB | MS.gene008900

M. sativa cultivar XinJiangDaYe / MS.gene008900

KEGG: K13425
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008900.t1	KEH20096.1	53.4	234	78	4	7	230	323	535	1.10E-54	223.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008900.t1	Q9SUS1	51.1	94	45	1	12	105	113	205	9.7e-20	98.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008900.t1	A0A072TSA8	53.4	234	78	4	7	230	323	535	7.8e-55	223.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene008900.t1	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008900.t1	MTR_8g067635	53.182	220	72	4	7	216	323	521	1.15e-64	209
MS.gene008900.t1	MTR_2g024380	46.914	243	108	5	9	230	227	469	2.10e-62	202
MS.gene008900.t1	MTR_8g067650	50.655	229	74	6	13	230	319	519	2.30e-60	198
MS.gene008900.t1	MTR_2g024350	46.429	224	97	4	29	230	4	226	2.26e-59	187
MS.gene008900.t1	MTR_6g053200	40.000	240	114	5	10	230	307	535	1.23e-44	156
MS.gene008900.t1	MTR_8g069935	40.000	220	65	3	11	230	310	462	5.15e-42	148
MS.gene008900.t1	MTR_7g104980	42.857	182	84	2	54	215	18	199	4.88e-41	139
MS.gene008900.t1	MTR_1g099600	50.505	99	48	1	11	109	163	260	4.32e-29	111
MS.gene008900.t1	MTR_8g028565	50.943	106	49	2	23	126	145	249	5.75e-27	106
MS.gene008900.t1	MTR_7g079040	50.000	96	47	1	10	105	131	225	6.48e-25	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene008900.t1	AT4G01250	54.217	83	37	1	23	105	118	199	7.11e-25	99.8
MS.gene008900.t1	AT5G52830	48.421	95	48	1	11	105	143	236	8.34e-25	100
MS.gene008900.t1	AT4G23550	51.685	89	42	1	17	105	124	211	2.37e-24	98.6
MS.gene008900.t1	AT5G45050	47.059	85	45	0	10	94	1018	1102	8.02e-21	91.3
MS.gene008900.t1	AT5G45050	47.059	85	45	0	10	94	1157	1241	9.13e-21	90.9
MS.gene008900.t1	AT5G45050	47.059	85	45	0	10	94	1129	1213	9.82e-21	90.9
MS.gene008900.t1	AT1G29280	58.209	67	26	1	34	98	75	141	1.87e-20	87.4
MS.gene008900.t1	AT2G34830	54.930	71	31	1	31	101	222	291	1.43e-19	87.0
MS.gene008900.t1	AT2G34830	54.930	71	31	1	31	101	213	282	1.46e-19	87.0
MS.gene008900.t1	AT3G58710	56.061	66	29	0	34	99	71	136	2.63e-19	84.3

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Find 40 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGACACCTATGTTAG+AGG	0.415313	8.2:-33911097	MS.gene008900:CDS
AAACGACCAAAATTTAAATC+AGG	0.434297	8.2:-33912066	MS.gene008900:CDS
AAACTTGGGTTCTCCTAATG+CGG	0.633652	8.2:-33911343	MS.gene008900:CDS
AAATGATAATCAAGTATCTT+TGG	0.332409	8.2:-33911903	MS.gene008900:CDS
AAGTTTGAATCATCTGTTGA+TGG	0.439589	8.2:-33911393	MS.gene008900:CDS
ACAATAAACCAGCAAATAAT+CGG	0.401514	8.2:-33911452	MS.gene008900:CDS
ACCCGAGCCTTGCTGCTCTC+TGG	0.394496	8.2:+33911300	None:intergenic
ACTCCATTGTCGATAGCTCT+CGG	0.621472	8.2:-33911428	MS.gene008900:CDS
ACTTAGTCCTTTACCTACAC+TGG	0.501454	8.2:-33911238	MS.gene008900:CDS
ACTTATGAAGTGACATATCT+AGG	0.442305	8.2:-33911486	MS.gene008900:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTTTGAATCATCTGTTGA+TGG	-	chr8.2:33911731-33911750	MS.gene008900:intron	30.0%
ACTTATGAAGTGACATATCT+AGG	-	chr8.2:33911638-33911657	MS.gene008900:intron	30.0%
ATGGACAAAAACCCATTAAA+GGG	-	chr8.2:33911298-33911317	MS.gene008900:CDS	30.0%
CATTGGAATCTTCATTATGT+TGG	+	chr8.2:33911928-33911947	None:intergenic	30.0%
TAAATGCAACTCTTTGCAAT+GGG	+	chr8.2:33911464-33911483	None:intergenic	30.0%
TAAATTTCGCTTCTGTATAC+GGG	+	chr8.2:33911973-33911992	None:intergenic	30.0%
TATGGACAAAAACCCATTAA+AGG	-	chr8.2:33911297-33911316	MS.gene008900:CDS	30.0%
TCAACAGATGATTCAAACTT+TGG	+	chr8.2:33911731-33911750	None:intergenic	30.0%
TTAAATGCAACTCTTTGCAA+TGG	+	chr8.2:33911465-33911484	None:intergenic	30.0%
TTGGAATCTTCATTATGTTG+GGG	+	chr8.2:33911926-33911945	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	33911047	33912099	33911047	ID=MS.gene008900
chr8.2	mRNA	33911047	33912099	33911047	ID=MS.gene008900.t1;Parent=MS.gene008900
chr8.2	exon	33912060	33912099	33912060	ID=MS.gene008900.t1.exon1;Parent=MS.gene008900.t1
chr8.2	CDS	33912060	33912099	33912060	ID=cds.MS.gene008900.t1;Parent=MS.gene008900.t1
chr8.2	exon	33911812	33911929	33911812	ID=MS.gene008900.t1.exon2;Parent=MS.gene008900.t1
chr8.2	CDS	33911812	33911929	33911812	ID=cds.MS.gene008900.t1;Parent=MS.gene008900.t1
chr8.2	exon	33911047	33911584	33911047	ID=MS.gene008900.t1.exon3;Parent=MS.gene008900.t1
chr8.2	CDS	33911047	33911584	33911047	ID=cds.MS.gene008900.t1;Parent=MS.gene008900.t1

Gene Sequence

>MS.gene008900

ATGCAAATCCAAAACGACCAAAATTTAAATCAGGAAAAAGGTAAAGTATTGATTCCTATTATTTAATATATTTTACATATAAGTATATTTCATTGCATTAAAATAATTATTTTTTTTACATATATTAACTCCTTGTGACCTAAAATCATGTTGCCTTTCTTTTCTTGTAGAAAAAAATGATAATCAAGTATCTTTGGTGAGCTATGTGGGGGCAAATAAGGTTTCAGAGGATTCATGGAGATGGAGAAAATATGGACAAAAACCCATTAAAGGGTCCTCACATCCAAGGTTTTTAAGCATTCAAAACTCTAACGTTCACATATTTTTCCTTCTTATATTAAAACAATATTTGTTACTTGATACACACACCCACACCAAGGGTTTTAATTAGTGAACGCGTGCATTTTGATCATTCCCATTGCAAAGAGTTGCATTTAAATGTGACAAATATGGTCTTAGCAATGAATGATTTTGCAATCAATGAATGATTTTGCAATCAATGAATGATTTTGCAGAAGCTACTACAAATGTAGCAGTTCCAGTGATTGTTTAGCAAGAAAACAAGTGGAAAAAATAAAAGATGGAGATGACACTTATGAAGTGACATATCTAGGAGAACACAATCACAATAAACCAGCAAATAATCGGAACTCCATTGTCGATAGCTCTCGGTACATGTCACCAAAGTTTGAATCATCTGTTGATGGAGAAGCTAGATGTTTCACAAACATCATAAACTTGGGTTCTCCTAATGCGGTAATGTTGCAGCTTGATCAGCCAGAGAGCAGCAAGGCTCGGGTTCTTGTTGGTCAATCAAAAATCCCTGATCTAAAAACTGAACTTAGTCCTTTACCTACACTGGGCAAAGCAAAATCTCCCCAACATAATGAAGATTCCAATGTTTTTTCATATGAATCAGAAATCCCGTATACAGAAGCGAAATTTATTGAAAGCTTCAAAGAAGATGAAATTTTGATACCAAACATGACACCTATGTTAGAGGAATTCTTTTTGGATTTCAACAATGTCAATAGTTGTGCTCTATTCCATTAG

Protein sequence

>MS.gene008900.t1

MQIQNDQNLNQEKEKNDNQVSLVSYVGANKVSEDSWRWRKYGQKPIKGSSHPRSYYKCSSSSDCLARKQVEKIKDGDDTYEVTYLGEHNHNKPANNRNSIVDSSRYMSPKFESSVDGEARCFTNIINLGSPNAVMLQLDQPESSKARVLVGQSKIPDLKTELSPLPTLGKAKSPQHNEDSNVFSYESEIPYTEAKFIESFKEDEILIPNMTPMLEEFFLDFNNVNSCALFH