AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009065

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009065.t1 MTR_8g065740 97.002 467 14 0 1 467 1 467 0.0 937
MS.gene009065.t1 MTR_5g048860 57.985 526 146 13 1 459 1 518 8.32e-177 507
MS.gene009065.t1 MTR_1g017350 42.671 307 152 7 114 420 92 374 2.57e-56 192
MS.gene009065.t1 MTR_1g017350 42.671 307 152 7 114 420 92 374 3.60e-56 192
MS.gene009065.t1 MTR_1g017350 41.693 319 146 9 114 415 92 387 9.91e-54 186
MS.gene009065.t1 MTR_1g017350 41.693 319 146 9 114 415 92 387 1.37e-53 186
MS.gene009065.t1 MTR_7g053410 44.444 288 131 10 125 393 128 405 3.96e-52 184
MS.gene009065.t1 MTR_3g116770 43.774 265 114 7 134 392 43 278 5.03e-52 181
MS.gene009065.t1 MTR_3g116770 43.774 265 114 7 134 392 43 278 5.42e-52 181
MS.gene009065.t1 MTR_7g053410 44.444 288 131 10 125 393 128 405 6.62e-52 184
MS.gene009065.t1 MTR_8g012290 38.757 338 160 15 126 430 93 416 9.47e-49 174
MS.gene009065.t1 MTR_8g012290 38.757 338 160 15 126 430 93 416 1.06e-48 174
MS.gene009065.t1 MTR_4g070320 71.963 107 28 1 288 392 124 230 1.10e-45 162
MS.gene009065.t1 MTR_8g062240 50.588 170 64 3 239 394 77 240 1.56e-41 149
MS.gene009065.t1 MTR_3g498825 85.366 82 12 0 296 377 138 219 9.93e-41 149
MS.gene009065.t1 MTR_5g037250 63.115 122 43 2 283 404 91 210 1.41e-40 145
MS.gene009065.t1 MTR_8g099880 82.353 85 14 1 301 384 141 225 3.39e-40 147
MS.gene009065.t1 MTR_7g092510 73.034 89 24 0 302 390 170 258 2.06e-39 145
MS.gene009065.t1 MTR_1g059270 68.085 94 30 0 302 395 157 250 6.90e-39 141
MS.gene009065.t1 MTR_1g059270 72.093 86 24 0 302 387 157 242 1.24e-37 140
MS.gene009065.t1 MTR_6g084120 52.252 111 42 2 276 384 124 225 3.20e-26 107
MS.gene009065.t1 MTR_4g131160 64.935 77 26 1 294 370 185 260 1.15e-21 97.1
MS.gene009065.t1 MTR_4g131160 64.935 77 26 1 294 370 188 263 1.33e-21 97.1
MS.gene009065.t1 MTR_1g080890 68.182 66 20 1 305 370 193 257 3.91e-20 92.0
MS.gene009065.t1 MTR_4g033295 55.385 65 29 0 305 369 118 182 2.33e-15 74.7
MS.gene009065.t1 MTR_4g065870 58.462 65 27 0 305 369 355 419 2.54e-15 78.2
MS.gene009065.t1 MTR_4g033295 53.731 67 30 1 305 370 118 184 3.83e-15 73.9
MS.gene009065.t1 MTR_2g098310 50.704 71 35 0 305 375 332 402 5.92e-15 77.0
MS.gene009065.t1 MTR_4g065870 58.065 62 26 0 305 366 355 416 1.32e-14 75.9
MS.gene009065.t1 MTR_2g098310 56.250 64 28 0 305 368 332 395 2.03e-14 75.5
MS.gene009065.t1 MTR_1g052470 52.174 69 33 0 305 373 298 366 2.90e-14 74.7
MS.gene009065.t1 MTR_7g092540 50.725 69 34 0 305 373 243 311 3.48e-14 73.9
MS.gene009065.t1 MTR_1g069155 54.688 64 28 1 307 370 319 381 4.07e-14 74.7
MS.gene009065.t1 MTR_1g084980 33.125 160 82 5 243 401 415 550 4.55e-14 75.1
MS.gene009065.t1 MTR_1g070870 63.636 66 23 1 305 370 120 184 1.24e-13 71.6
MS.gene009065.t1 MTR_1g070870 63.636 66 23 1 305 370 123 187 1.39e-13 71.6
MS.gene009065.t1 MTR_7g092540 50.769 65 32 0 305 369 243 307 1.72e-13 71.6
MS.gene009065.t1 MTR_3g028520 50.769 65 32 0 305 369 322 386 8.70e-13 70.1
MS.gene009065.t1 MTR_3g027650 50.769 65 32 0 305 369 322 386 8.70e-13 70.1
MS.gene009065.t1 MTR_7g099540 51.562 64 30 1 307 370 254 316 1.63e-12 69.7
MS.gene009065.t1 MTR_1g019240 43.210 81 40 2 291 371 76 150 2.00e-12 67.8
MS.gene009065.t1 MTR_3g027650 51.613 62 30 0 305 366 322 383 4.88e-12 67.8
MS.gene009065.t1 MTR_8g036235 47.692 65 34 0 305 369 316 380 8.23e-12 67.0
MS.gene009065.t1 MTR_1g018090 50.769 65 31 1 305 369 274 337 1.88e-11 65.9
MS.gene009065.t1 MTR_7g099540 48.438 64 32 1 307 370 254 316 3.77e-11 65.5
MS.gene009065.t1 MTR_3g449770 50.000 64 31 1 307 370 311 373 4.05e-11 65.5
MS.gene009065.t1 MTR_5g017040 53.571 56 25 1 316 371 116 170 6.31e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009065.t1 AT1G68920 44.592 453 196 15 17 454 28 440 3.20e-97 301
MS.gene009065.t1 AT1G68920 44.592 453 197 15 17 454 28 441 3.91e-97 301
MS.gene009065.t1 AT1G68920 44.592 453 197 15 17 454 28 441 3.91e-97 301
MS.gene009065.t1 AT1G68920 44.592 453 197 15 17 454 28 441 3.91e-97 301
MS.gene009065.t1 AT1G26260 38.926 447 172 15 1 440 1 353 3.90e-69 225
MS.gene009065.t1 AT1G26260 38.926 447 172 15 1 440 1 353 3.90e-69 225
MS.gene009065.t1 AT1G26260 38.926 447 172 15 1 440 1 353 3.90e-69 225
MS.gene009065.t1 AT1G26260 38.926 447 172 15 1 440 1 353 3.90e-69 225
MS.gene009065.t1 AT1G26260 46.006 313 122 9 134 440 60 331 1.33e-65 216
MS.gene009065.t1 AT1G26260 51.709 234 86 4 207 440 96 302 1.59e-63 209
MS.gene009065.t1 AT1G10120 52.083 192 82 2 213 404 124 305 4.20e-54 185
MS.gene009065.t1 AT1G10120 52.083 192 82 2 213 404 124 305 4.20e-54 185
MS.gene009065.t1 AT3G07340 41.319 288 105 8 106 392 121 345 1.03e-48 173
MS.gene009065.t1 AT3G07340 41.319 288 105 8 106 392 121 345 1.49e-48 173
MS.gene009065.t1 AT5G48560 40.370 270 130 8 126 392 148 389 3.01e-46 167
MS.gene009065.t1 AT5G48560 40.370 270 130 8 126 392 148 389 7.14e-46 167
MS.gene009065.t1 AT3G23690 49.065 214 62 8 262 436 109 314 5.86e-45 161
MS.gene009065.t1 AT4G34530 68.750 112 30 2 302 408 169 280 7.98e-45 160
MS.gene009065.t1 AT2G18300 54.110 146 62 1 239 384 124 264 3.10e-42 153
MS.gene009065.t1 AT5G50915 56.410 156 49 6 260 413 106 244 3.36e-42 151
MS.gene009065.t1 AT5G50915 56.410 156 49 6 260 413 106 244 3.36e-42 151
MS.gene009065.t1 AT2G18300 54.110 146 62 1 239 384 124 264 3.91e-42 152
MS.gene009065.t1 AT5G50915 56.410 156 49 6 260 413 106 244 4.99e-42 151
MS.gene009065.t1 AT5G50915 56.410 156 49 6 260 413 106 244 4.99e-42 151
MS.gene009065.t1 AT2G18300 54.110 146 62 1 239 384 124 264 7.43e-42 152
MS.gene009065.t1 AT1G59640 65.455 110 37 1 285 394 117 225 6.88e-41 147
MS.gene009065.t1 AT1G59640 42.424 198 108 3 196 392 31 223 1.02e-40 149
MS.gene009065.t1 AT4G36540 51.974 152 64 2 268 410 101 252 2.33e-40 147
MS.gene009065.t1 AT4G36540 59.200 125 48 1 268 389 101 225 2.55e-39 144
MS.gene009065.t1 AT2G42300 67.778 90 29 0 302 391 182 271 1.53e-38 141
MS.gene009065.t1 AT3G57800 73.256 86 23 0 302 387 201 286 1.34e-37 141
MS.gene009065.t1 AT2G42300 68.539 89 28 0 302 390 182 270 2.77e-37 139
MS.gene009065.t1 AT5G62610 74.074 81 20 1 296 376 145 224 1.07e-35 134
MS.gene009065.t1 AT5G62610 74.074 81 20 1 296 376 145 224 1.07e-35 134
MS.gene009065.t1 AT1G73830 61.321 106 41 0 279 384 121 226 2.07e-34 130
MS.gene009065.t1 AT1G73830 61.321 106 41 0 279 384 121 226 2.13e-34 130
MS.gene009065.t1 AT1G18400 62.264 106 35 2 303 406 143 245 2.63e-34 129
MS.gene009065.t1 AT1G18400 74.390 82 21 0 303 384 143 224 4.37e-34 129
MS.gene009065.t1 AT1G25330 52.713 129 55 2 269 393 66 192 4.65e-34 127
MS.gene009065.t1 AT3G57800 47.368 133 23 1 302 387 201 333 5.79e-29 118
MS.gene009065.t1 AT2G24260 59.091 88 33 2 283 370 15 99 6.24e-23 97.8
MS.gene009065.t1 AT4G30980 56.122 98 37 2 273 370 103 194 1.97e-22 97.8
MS.gene009065.t1 AT4G30980 56.122 98 37 2 273 370 151 242 4.00e-22 97.8
MS.gene009065.t1 AT2G24260 59.091 88 33 2 283 370 118 202 4.45e-22 97.8
MS.gene009065.t1 AT5G58010 70.149 67 19 1 304 370 98 163 3.90e-21 94.0
MS.gene009065.t1 AT2G42300 81.395 43 8 0 302 344 182 224 4.52e-17 80.5
MS.gene009065.t1 AT2G42300 81.395 43 8 0 302 344 182 224 4.52e-17 80.5
MS.gene009065.t1 AT2G42300 81.395 43 8 0 302 344 182 224 5.44e-17 80.5
MS.gene009065.t1 AT2G20180 40.909 110 57 3 261 370 170 271 4.48e-16 80.5
MS.gene009065.t1 AT2G20180 40.909 110 57 3 261 370 170 271 4.48e-16 80.5
MS.gene009065.t1 AT2G20180 40.909 110 57 3 261 370 170 271 4.48e-16 80.5
MS.gene009065.t1 AT2G20180 40.909 110 57 3 261 370 241 342 4.51e-16 80.9
MS.gene009065.t1 AT2G20180 40.909 110 57 3 261 370 241 342 4.51e-16 80.9
MS.gene009065.t1 AT2G20180 40.909 110 57 3 261 370 241 342 4.51e-16 80.9
MS.gene009065.t1 AT2G20180 40.909 110 57 3 261 370 241 342 4.51e-16 80.9
MS.gene009065.t1 AT2G20180 40.187 107 56 3 261 367 241 339 7.13e-15 76.3
MS.gene009065.t1 AT4G28790 33.562 146 82 4 274 415 236 370 1.05e-14 76.3
MS.gene009065.t1 AT1G51140 56.250 64 28 0 305 368 304 367 1.88e-14 75.1
MS.gene009065.t1 AT4G28800 30.151 199 115 7 267 462 229 406 2.04e-14 75.5
MS.gene009065.t1 AT1G35460 52.308 65 31 0 305 369 181 245 5.69e-14 72.4
MS.gene009065.t1 AT4G09180 51.515 66 32 0 304 369 183 248 6.70e-14 72.0
MS.gene009065.t1 AT2G42280 50.769 65 32 0 305 369 279 343 1.00e-13 72.8
MS.gene009065.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.12e-13 73.6
MS.gene009065.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.12e-13 73.6
MS.gene009065.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.12e-13 73.6
MS.gene009065.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.12e-13 73.6
MS.gene009065.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.12e-13 73.6
MS.gene009065.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.12e-13 73.6
MS.gene009065.t1 AT4G28815 56.897 58 24 1 308 365 71 127 1.96e-13 70.1
MS.gene009065.t1 AT4G28800 28.713 202 108 6 267 462 241 412 1.97e-13 72.4
MS.gene009065.t1 AT2G42280 53.030 66 29 1 305 368 279 344 2.19e-13 72.0
MS.gene009065.t1 AT4G02590 62.121 66 24 1 305 370 83 147 4.02e-13 69.7
MS.gene009065.t1 AT4G28815 56.897 58 24 1 308 365 147 203 5.75e-13 70.1
MS.gene009065.t1 AT4G02590 62.121 66 24 1 305 370 146 210 5.86e-13 70.1
MS.gene009065.t1 AT4G02590 62.121 66 24 1 305 370 146 210 5.86e-13 70.1
MS.gene009065.t1 AT1G03040 62.121 66 24 1 305 370 139 203 6.94e-13 69.7
MS.gene009065.t1 AT1G03040 62.121 66 24 1 305 370 144 208 8.22e-13 69.3
MS.gene009065.t1 AT1G03040 62.121 66 24 1 305 370 144 208 1.53e-12 68.9
MS.gene009065.t1 AT4G28790 34.951 103 62 2 274 372 236 337 3.13e-12 68.2
MS.gene009065.t1 AT4G00050 49.275 69 34 1 301 369 101 168 5.32e-12 67.0
MS.gene009065.t1 AT1G27740 37.398 123 60 4 263 369 109 230 5.54e-12 66.2
MS.gene009065.t1 AT2G46970 34.513 113 68 2 303 410 216 327 5.82e-12 67.8
MS.gene009065.t1 AT2G46970 34.513 113 68 2 303 410 185 296 5.91e-12 67.8
MS.gene009065.t1 AT2G46970 34.513 113 68 2 303 410 194 305 7.17e-12 67.4
MS.gene009065.t1 AT4G00050 49.275 69 34 1 301 369 203 270 1.33e-11 66.6
MS.gene009065.t1 AT4G00050 49.275 69 34 1 301 369 203 270 1.56e-11 66.2
MS.gene009065.t1 AT2G43140 50.000 64 32 0 305 368 231 294 3.27e-11 64.7
MS.gene009065.t1 AT2G43140 50.000 64 32 0 305 368 134 197 3.89e-11 63.2
MS.gene009065.t1 AT4G33880 49.231 65 32 1 305 369 266 329 4.69e-11 64.7
MS.gene009065.t1 AT4G36930 50.820 61 29 1 311 371 197 256 6.95e-11 64.3

Find 127 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATATGGTCCTAATTTCT+TGG 0.233035 8.2:-36585134 MS.gene009065:CDS
TGCAATGGATTAACCTGATT+TGG 0.278397 8.2:+36582597 None:intergenic
AGTGGAGAACCTTCTATTAA+AGG 0.288877 8.2:-36584608 MS.gene009065:CDS
TATGAATCTACCATTGAATT+AGG 0.293515 8.2:+36585151 None:intergenic
GCTTCAACAACCAAGGCTTC+TGG 0.303821 8.2:-36583965 MS.gene009065:CDS
CCTTCATCTTGAGACATTAC+AGG 0.318475 8.2:+36584716 None:intergenic
GCTCTCACAGTTGTCCTTTG+TGG 0.319104 8.2:+36584012 None:intergenic
GACTAGAACCATACATCTTT+AGG 0.325629 8.2:+36582896 None:intergenic
CACTAGCACAATCCAACATT+TGG 0.335451 8.2:+36584627 None:intergenic
TGCAAATTCATCTGTAGGTT+TGG 0.339897 8.2:-36585212 MS.gene009065:CDS
ACCGGGTCCATCATCTGCTC+TGG 0.343461 8.2:-36582778 MS.gene009065:CDS
GGATTTAGACCTTTAATAGA+AGG 0.344206 8.2:+36584599 None:intergenic
GTAAGAAGGTCCAAGAAATT+AGG 0.353442 8.2:+36585124 None:intergenic
GAAAGGAGGGTCCTTTAGAT+TGG 0.355470 8.2:-36585255 MS.gene009065:CDS
TCAACCACAGTTGGAACCTT+TGG 0.357269 8.2:-36582637 MS.gene009065:CDS
TTCTGGGAAGAATGCCAAAC+AGG 0.361984 8.2:-36583948 MS.gene009065:CDS
ACGAGGGATACGTACATGTT+AGG 0.367434 8.2:-36583901 MS.gene009065:CDS
TCAGATTCTTCAACATCAAC+CGG 0.371564 8.2:-36582796 MS.gene009065:intron
TCTTACTAACTCCGAAAACT+TGG 0.372119 8.2:-36585107 MS.gene009065:CDS
TCTGAGAAATTTGCCATGTT+AGG 0.374371 8.2:+36582678 None:intergenic
AATCACTTCTTCGATCAATC+TGG 0.381385 8.2:+36584741 None:intergenic
GGCATTCTTCCCAGAAGCCT+TGG 0.393219 8.2:+36583955 None:intergenic
ATCTGTAGGTTTGGTTTCCA+TGG 0.397148 8.2:-36585203 MS.gene009065:CDS
CTTCAACAACCAAGGCTTCT+GGG 0.404138 8.2:-36583964 MS.gene009065:CDS
TTCCATGGAAAATTCTATGA+TGG 0.411759 8.2:-36585188 MS.gene009065:CDS
GGTTGATATGAATGTGATAA+TGG 0.412278 8.2:+36582723 None:intergenic
GTGTGGGATTCCTCAATCTA+TGG 0.414177 8.2:-36584822 MS.gene009065:CDS
GTTGAGGAAATTTCAGTCCT+TGG 0.419505 8.2:+36582569 None:intergenic
GGAAATATCTCAGCCCCATT+TGG 0.420071 8.2:+36584941 None:intergenic
CAGTCCTTGGCTGCTTGCAA+TGG 0.420935 8.2:+36582582 None:intergenic
CTTCTTCGATCAATCTGGAT+TGG 0.432794 8.2:+36584746 None:intergenic
TTCTTCATGTCCTAATTCAA+TGG 0.433965 8.2:-36585161 MS.gene009065:CDS
CCAAAGAAAAGGAAAAGAAA+TGG 0.434507 8.2:-36584578 MS.gene009065:CDS
GAAAACTGAGACAAACTTTG+AGG 0.438701 8.2:+36584920 None:intergenic
CCGGGTCCATCATCTGCTCT+GGG 0.439623 8.2:-36582777 MS.gene009065:CDS
AGCAATGAAGTGAGAAAAGA+TGG 0.442777 8.2:-36585058 MS.gene009065:CDS
ATGGGTTGGGACCTTGGTAG+TGG 0.442811 8.2:-36584992 MS.gene009065:CDS
TTATTCTGTTTGTAGGTCAC+TGG 0.450051 8.2:-36583596 MS.gene009065:intron
TCTCGGTCTGTTGAGCATCT+TGG 0.450146 8.2:-36584782 MS.gene009065:CDS
GTTGGAATGGGTTGGGACCT+TGG 0.452583 8.2:-36584998 MS.gene009065:CDS
CACTGGCAAGGCAGTGATGC+TGG 0.457604 8.2:-36583579 MS.gene009065:CDS
ACTTGGTAGCTCAAGAGTTC+CGG 0.459094 8.2:+36584039 None:intergenic
TTTCTTCAAGATCTTGTACC+TGG 0.462998 8.2:-36583716 MS.gene009065:CDS
AGGGTTTAAAGAGCCAAATC+AGG 0.463980 8.2:-36582610 MS.gene009065:CDS
GAAGTGAGAAAAGATGGGTT+TGG 0.476292 8.2:-36585052 MS.gene009065:CDS
ACTAGCACAATCCAACATTT+GGG 0.480346 8.2:+36584628 None:intergenic
CGAGGGATACGTACATGTTA+GGG 0.480619 8.2:-36583900 MS.gene009065:CDS
AGAAGCTTGAGACCCCTGTT+TGG 0.481555 8.2:+36583934 None:intergenic
AAGGTCTAAATCCAAAGAAA+AGG 0.482803 8.2:-36584589 MS.gene009065:CDS
AATTCAATGGTAGATTCATA+TGG 0.486689 8.2:-36585148 MS.gene009065:CDS
CCACAGTTGGAACCTTTGGC+TGG 0.487866 8.2:-36582633 MS.gene009065:CDS
AGTTGGAACCTTTGGCTGGA+GGG 0.488222 8.2:-36582629 MS.gene009065:CDS
AAATGTACCTTGTTGCATCC+AGG 0.496384 8.2:+36583698 None:intergenic
CTCTACCTCTACATGTGTCT+CGG 0.501570 8.2:-36584799 MS.gene009065:CDS
TAGAACAGTTGGAATGGGTT+GGG 0.502266 8.2:-36585005 MS.gene009065:CDS
AGGAGGGTCCTTTAGATTGG+AGG 0.508576 8.2:-36585252 MS.gene009065:CDS
TCCATGGAAAATTCTATGAT+GGG 0.513525 8.2:-36585187 MS.gene009065:CDS
GAAAAGGAAAAGAAATGGAC+AGG 0.513621 8.2:-36584573 MS.gene009065:intron
TTGTCCTTTGTGGTTTCACT+TGG 0.513815 8.2:+36584022 None:intergenic
GGCTATTTGTTGCTTGACCA+CGG 0.515569 8.2:+36583873 None:intergenic
TTACTCTCTCTGCAAGGCTA+TGG 0.516396 8.2:+36583852 None:intergenic
CTCATAAACCTCCAATCTAA+AGG 0.517400 8.2:+36585244 None:intergenic
CCTGTAATGTCTCAAGATGA+AGG 0.522379 8.2:-36584716 MS.gene009065:CDS
AGAAACCCCAGAGCAGATGA+TGG 0.522386 8.2:+36582771 None:intergenic
GATGAAGGGAAACAAGCTCT+TGG 0.524819 8.2:-36584701 MS.gene009065:CDS
GCAGACTGTTCAACCACAGT+TGG 0.533598 8.2:-36582646 MS.gene009065:CDS
CAAATTTCTCAGATATGCTT+AGG 0.541933 8.2:-36582668 MS.gene009065:CDS
TGATGATAGAACAGTTGGAA+TGG 0.544564 8.2:-36585011 MS.gene009065:CDS
TGATGATGGTTCCCAAATGT+TGG 0.546620 8.2:-36584639 MS.gene009065:CDS
ATAGAACAGTTGGAATGGGT+TGG 0.546913 8.2:-36585006 MS.gene009065:CDS
GAAGGGAAACAAGCTCTTGG+AGG 0.547800 8.2:-36584698 MS.gene009065:CDS
GGTTGTGATGATAGAACAGT+TGG 0.548953 8.2:-36585016 MS.gene009065:CDS
AACCTTGCAAATTCATCTGT+AGG 0.548963 8.2:-36585217 MS.gene009065:CDS
TAATCCATTGCAAGCAGCCA+AGG 0.549245 8.2:-36582586 MS.gene009065:CDS
GTTGAGCATCTTGGTAGTGA+TGG 0.549328 8.2:-36584773 MS.gene009065:CDS
TCTTTAAACCCTCCAGCCAA+AGG 0.552371 8.2:+36582621 None:intergenic
CAAATGTTGGATTGTGCTAG+TGG 0.554215 8.2:-36584626 MS.gene009065:CDS
CCCAGAGCAGATGATGGACC+CGG 0.555611 8.2:+36582777 None:intergenic
CAGTTGGAACCTTTGGCTGG+AGG 0.557432 8.2:-36582630 MS.gene009065:CDS
CATCATATCCACGAAACTCC+CGG 0.562237 8.2:+36584850 None:intergenic
GATGATAGAACAGTTGGAAT+GGG 0.563426 8.2:-36585010 MS.gene009065:CDS
AGAGGTAGAGCCATAGATTG+AGG 0.574801 8.2:+36584812 None:intergenic
CTGTAATGTCTCAAGATGAA+GGG 0.576780 8.2:-36584715 MS.gene009065:CDS
AGAGTTGAATGAATCAGTCC+TGG 0.580047 8.2:+36582702 None:intergenic
CTTACTAACTCCGAAAACTT+GGG 0.581398 8.2:-36585106 MS.gene009065:CDS
ACTAACTCCGAAAACTTGGG+TGG 0.585659 8.2:-36585103 MS.gene009065:CDS
GATGAATCAAGTGGTGATGA+TGG 0.586435 8.2:-36584653 MS.gene009065:CDS
AGCTTCTGATCCGCCTAACG+AGG 0.590451 8.2:-36583918 MS.gene009065:CDS
TCTGGGAAGAATGCCAAACA+GGG 0.593622 8.2:-36583947 MS.gene009065:CDS
ACATGTACGTATCCCTCGTT+AGG 0.594600 8.2:+36583905 None:intergenic
AAGGGAAACAAGCTCTTGGA+GGG 0.596219 8.2:-36584697 MS.gene009065:CDS
GTAGTGGTTCTATGATGAAG+AGG 0.598345 8.2:-36584976 MS.gene009065:CDS
CTGGATTTATTGATGCTGCA+AGG 0.604609 8.2:-36584889 MS.gene009065:CDS
TTTGAGGTAGATAGAAAGGA+GGG 0.606702 8.2:-36585268 MS.gene009065:CDS
CTGTTTGTAGGTCACTGGCA+AGG 0.607483 8.2:-36583591 MS.gene009065:intron
TCCATATTAAAATCCACATG+TGG 0.609814 8.2:+36582929 None:intergenic
GATAATGGAGGAAAAGCCAT+TGG 0.610652 8.2:+36582738 None:intergenic
TCATTCAACTCTACCTAACA+TGG 0.611728 8.2:-36582691 MS.gene009065:CDS
AACCTACAGATGAATTTGCA+AGG 0.617947 8.2:+36585215 None:intergenic
CAGATTCTTCAACATCAACC+GGG 0.618225 8.2:-36582795 MS.gene009065:CDS
TGGTAGCTCAAGAGTTCCGG+TGG 0.619141 8.2:+36584042 None:intergenic
CCAGCCAAAGGTTCCAACTG+TGG 0.623573 8.2:+36582633 None:intergenic
GGGTCTTGTAATGAAGCTGA+TGG 0.623907 8.2:-36584677 MS.gene009065:CDS
TAGTGGTTCTATGATGAAGA+GGG 0.626282 8.2:-36584975 MS.gene009065:CDS
TGTACGTATCCCTCGTTAGG+CGG 0.628257 8.2:+36583908 None:intergenic
AACATCTTACTCTCTCTGCA+AGG 0.632535 8.2:+36583846 None:intergenic
TGGATATGATGAACTCGTGT+GGG 0.632999 8.2:-36584838 MS.gene009065:CDS
GTCTCTTCAACAACAAGTTG+AGG 0.634721 8.2:-36583534 MS.gene009065:intron
GGTGATAGTGATAAAGCCAC+CGG 0.644324 8.2:-36584058 MS.gene009065:CDS
TTAGATTGGAGGTTTATGAG+TGG 0.645386 8.2:-36585241 MS.gene009065:CDS
TGCAACAGTAAATCCACATG+TGG 0.646636 8.2:-36582942 MS.gene009065:CDS
GCTTCTGATCCGCCTAACGA+GGG 0.648015 8.2:-36583917 MS.gene009065:CDS
GCAAACAGCTTCAACAACCA+AGG 0.656445 8.2:-36583972 MS.gene009065:CDS
TGATATGAATGTGATAATGG+AGG 0.657822 8.2:+36582726 None:intergenic
GCAATGAAGTGAGAAAAGAT+GGG 0.658069 8.2:-36585057 MS.gene009065:CDS
GGTTCTATGATGAAGAGGGA+TGG 0.658101 8.2:-36584971 MS.gene009065:CDS
CGGGTCCATCATCTGCTCTG+GGG 0.660359 8.2:-36582776 MS.gene009065:CDS
GTTTCTCAGAGAAATGCCAA+TGG 0.663274 8.2:-36582754 MS.gene009065:CDS
GTGGATATGATGAACTCGTG+TGG 0.663347 8.2:-36584839 MS.gene009065:CDS
TCTTGTACCTGGATGCAACA+AGG 0.668293 8.2:-36583705 MS.gene009065:intron
ACAGACCGAGACACATGTAG+AGG 0.676355 8.2:+36584794 None:intergenic
TCATCATAGAACCACTACCA+AGG 0.687049 8.2:+36584981 None:intergenic
TTATCACATTCATATCAACC+AGG 0.710399 8.2:-36582720 MS.gene009065:CDS
GCTACCAAGTGAAACCACAA+AGG 0.714013 8.2:-36584026 MS.gene009065:CDS
CTGGGAAGAATGCCAAACAG+GGG 0.714387 8.2:-36583946 MS.gene009065:CDS
GCTGATGGAGATGAATCAAG+TGG 0.739157 8.2:-36584662 MS.gene009065:CDS
GTACATGTTAGGGCTCGCCG+TGG 0.768171 8.2:-36583890 MS.gene009065:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTTTATTTTCTGTT+AGG - chr8.2:36583784-36583803 MS.gene009065:intron 10.0%
!! CAAAATATTCAATTTCATAA+AGG + chr8.2:36585051-36585070 None:intergenic 15.0%
!! TAAAATAAAATAAACTCACA+TGG + chr8.2:36583775-36583794 None:intergenic 15.0%
!!! ATTTCTAGAAACATATATAA+GGG + chr8.2:36584838-36584857 None:intergenic 15.0%
!! AAAATTCATCATAGTGAATT+GGG - chr8.2:36583611-36583630 MS.gene009065:intron 20.0%
!! ATTATTTAGATTTCAACTGT+AGG - chr8.2:36584096-36584115 MS.gene009065:intron 20.0%
!! GATGAGAAAAAAAAATTGTT+TGG - chr8.2:36584060-36584079 MS.gene009065:CDS 20.0%
!! GTCATTAATCAAATGAAAAA+AGG - chr8.2:36583537-36583556 MS.gene009065:CDS 20.0%
!! TAAATCCTCATATGAATATA+GGG + chr8.2:36584222-36584241 None:intergenic 20.0%
!!! ATAAGATAAACTGTTTTGTT+AGG - chr8.2:36584796-36584815 MS.gene009065:CDS 20.0%
!!! GATTTCTAGAAACATATATA+AGG + chr8.2:36584839-36584858 None:intergenic 20.0%
!!! TATTTTATTTTCTGTTAGGA+TGG - chr8.2:36583788-36583807 MS.gene009065:intron 20.0%
!!! TTTTCATTTGATTAATGACT+TGG + chr8.2:36583536-36583555 None:intergenic 20.0%
! AAAAATATGGGAAAATCAGT+TGG + chr8.2:36584401-36584420 None:intergenic 25.0%
! AATTCAATGGTAGATTCATA+TGG - chr8.2:36582719-36582738 MS.gene009065:CDS 25.0%
! ATATCCAAAATAGATACACT+AGG - chr8.2:36583650-36583669 MS.gene009065:intron 25.0%
! CAAAATTCATCATAGTGAAT+TGG - chr8.2:36583610-36583629 MS.gene009065:intron 25.0%
! CTAAATCCTCATATGAATAT+AGG + chr8.2:36584223-36584242 None:intergenic 25.0%
! GAAGCAGAATACAAAAATAT+GGG + chr8.2:36584413-36584432 None:intergenic 25.0%
! TATCCAAAATAGATACACTA+GGG - chr8.2:36583651-36583670 MS.gene009065:intron 25.0%
! TATGAATCTACCATTGAATT+AGG + chr8.2:36582719-36582738 None:intergenic 25.0%
! TGAAGCAGAATACAAAAATA+TGG + chr8.2:36584414-36584433 None:intergenic 25.0%
! TTGGATGATTTAATAATCAG+TGG + chr8.2:36584382-36584401 None:intergenic 25.0%
!! ATCATTGTTATTCTGTTTGT+AGG - chr8.2:36584264-36584283 MS.gene009065:intron 25.0%
!! TTTTAGTATATTTGATACCG+TGG - chr8.2:36583337-36583356 MS.gene009065:intron 25.0%
!!! AAATTTTGAGGTAGATAGAA+AGG - chr8.2:36582595-36582614 MS.gene009065:CDS 25.0%
!!! ATGTGGATTTTAATATGGAA+CGG - chr8.2:36584942-36584961 MS.gene009065:CDS 25.0%
!!! ATTGTAAGCTTTAAAATTGC+TGG + chr8.2:36585007-36585026 None:intergenic 25.0%
AAAATAAACTCACATGGACA+AGG + chr8.2:36583769-36583788 None:intergenic 30.0%
AAATAAACTCACATGGACAA+GGG + chr8.2:36583768-36583787 None:intergenic 30.0%
AAGGTCTAAATCCAAAGAAA+AGG - chr8.2:36583278-36583297 MS.gene009065:intron 30.0%
AATTGTAACAAAGATGACAC+TGG + chr8.2:36584755-36584774 None:intergenic 30.0%
AGTGTCATCTTTGTTACAAT+TGG - chr8.2:36584754-36584773 MS.gene009065:CDS 30.0%
ATATAAGGGACAACTAACAA+AGG + chr8.2:36584824-36584843 None:intergenic 30.0%
ATGTCAATAAGAAACTCTAC+TGG + chr8.2:36584657-36584676 None:intergenic 30.0%
ATTGATAAAAACTGCCAAGA+GGG + chr8.2:36584899-36584918 None:intergenic 30.0%
CAAATTTCTCAGATATGCTT+AGG - chr8.2:36585199-36585218 MS.gene009065:CDS 30.0%
CCAAAGAAAAGGAAAAGAAA+TGG - chr8.2:36583289-36583308 MS.gene009065:intron 30.0%
GGATTTAGACCTTTAATAGA+AGG + chr8.2:36583271-36583290 None:intergenic 30.0%
GTGAATTGGGATTAAAAATG+AGG - chr8.2:36583624-36583643 MS.gene009065:intron 30.0%
TCCATATTAAAATCCACATG+TGG + chr8.2:36584941-36584960 None:intergenic 30.0%
TCCATGGAAAATTCTATGAT+GGG - chr8.2:36582680-36582699 MS.gene009065:CDS 30.0%
TTATCACATTCATATCAACC+AGG - chr8.2:36585147-36585166 MS.gene009065:CDS 30.0%
TTCATATGGTCCTAATTTCT+TGG - chr8.2:36582733-36582752 MS.gene009065:CDS 30.0%
TTCCATGGAAAATTCTATGA+TGG - chr8.2:36582679-36582698 MS.gene009065:CDS 30.0%
TTCTTCATGTCCTAATTCAA+TGG - chr8.2:36582706-36582725 MS.gene009065:CDS 30.0%
TTGAGGTTTAAATCGTGAAT+CGG - chr8.2:36583368-36583387 MS.gene009065:intron 30.0%
! AAACCTCAAATTGATAACCA+CGG + chr8.2:36583357-36583376 None:intergenic 30.0%
! AATCCCTAGTGTATCTATTT+TGG + chr8.2:36583657-36583676 None:intergenic 30.0%
! ACTAGAGCTACACTAATTTT+AGG - chr8.2:36583566-36583585 MS.gene009065:CDS 30.0%
! CCATTTCTTTTCCTTTTCTT+TGG + chr8.2:36583292-36583311 None:intergenic 30.0%
! GAGTGAAAGTGAAAATTTTG+AGG - chr8.2:36582583-36582602 MS.gene009065:CDS 30.0%
! GGTTGATATGAATGTGATAA+TGG + chr8.2:36585147-36585166 None:intergenic 30.0%
! GTACATTTAAAACTTGAGAC+TGG - chr8.2:36584184-36584203 MS.gene009065:intron 30.0%
!! TCCACATGTGGATTTTAATA+TGG - chr8.2:36584937-36584956 MS.gene009065:CDS 30.0%
!! TGATATGAATGTGATAATGG+AGG + chr8.2:36585144-36585163 None:intergenic 30.0%
AACCTACAGATGAATTTGCA+AGG + chr8.2:36582655-36582674 None:intergenic 35.0%
AACCTTGCAAATTCATCTGT+AGG - chr8.2:36582650-36582669 MS.gene009065:CDS 35.0%
AACTGTGAGAGCAGAAAAAA+GGG - chr8.2:36583866-36583885 MS.gene009065:CDS 35.0%
AATAAACTCACATGGACAAG+GGG + chr8.2:36583767-36583786 None:intergenic 35.0%
AATCACTTCTTCGATCAATC+TGG + chr8.2:36583129-36583148 None:intergenic 35.0%
ACTAGCACAATCCAACATTT+GGG + chr8.2:36583242-36583261 None:intergenic 35.0%
AGCAATGAAGTGAGAAAAGA+TGG - chr8.2:36582809-36582828 MS.gene009065:intron 35.0%
AGTGGAGAACCTTCTATTAA+AGG - chr8.2:36583259-36583278 MS.gene009065:intron 35.0%
ATACCGTGGTTATCAATTTG+AGG - chr8.2:36583351-36583370 MS.gene009065:intron 35.0%
CAATTTCATAAAGGTCAGTC+AGG + chr8.2:36585042-36585061 None:intergenic 35.0%
CACTACCCTATATTCATATG+AGG - chr8.2:36584214-36584233 MS.gene009065:intron 35.0%
CATTGATAAAAACTGCCAAG+AGG + chr8.2:36584900-36584919 None:intergenic 35.0%
CTCATAAACCTCCAATCTAA+AGG + chr8.2:36582626-36582645 None:intergenic 35.0%
CTGTAATGTCTCAAGATGAA+GGG - chr8.2:36583152-36583171 MS.gene009065:intron 35.0%
CTTACTAACTCCGAAAACTT+GGG - chr8.2:36582761-36582780 MS.gene009065:CDS 35.0%
GAAAACTGAGACAAACTTTG+AGG + chr8.2:36582950-36582969 None:intergenic 35.0%
GAAAAGGAAAAGAAATGGAC+AGG - chr8.2:36583294-36583313 MS.gene009065:intron 35.0%
GACTAGAACCATACATCTTT+AGG + chr8.2:36584974-36584993 None:intergenic 35.0%
GATGATAGAACAGTTGGAAT+GGG - chr8.2:36582857-36582876 MS.gene009065:intron 35.0%
GCAATGAAGTGAGAAAAGAT+GGG - chr8.2:36582810-36582829 MS.gene009065:intron 35.0%
GTAAGAAGGTCCAAGAAATT+AGG + chr8.2:36582746-36582765 None:intergenic 35.0%
TAGCTGTTATTCGACAACAT+AGG - chr8.2:36584606-36584625 MS.gene009065:CDS 35.0%
TCAGATTCTTCAACATCAAC+CGG - chr8.2:36585071-36585090 MS.gene009065:CDS 35.0%
TCATTCAACTCTACCTAACA+TGG - chr8.2:36585176-36585195 MS.gene009065:CDS 35.0%
TCTGAGAAATTTGCCATGTT+AGG + chr8.2:36585192-36585211 None:intergenic 35.0%
TCTTACTAACTCCGAAAACT+TGG - chr8.2:36582760-36582779 MS.gene009065:CDS 35.0%
TGATGATAGAACAGTTGGAA+TGG - chr8.2:36582856-36582875 MS.gene009065:intron 35.0%
TGCAAATTCATCTGTAGGTT+TGG - chr8.2:36582655-36582674 MS.gene009065:CDS 35.0%
TTAGATTGGAGGTTTATGAG+TGG - chr8.2:36582626-36582645 MS.gene009065:CDS 35.0%
TTAGATTTCAACTGTAGGTG+AGG - chr8.2:36584101-36584120 MS.gene009065:intron 35.0%
TTATTCTGTTTGTAGGTCAC+TGG - chr8.2:36584271-36584290 MS.gene009065:intron 35.0%
TTTCTTCAAGATCTTGTACC+TGG - chr8.2:36584151-36584170 MS.gene009065:intron 35.0%
! ACAAAAACCACCCAAGTTTT+CGG + chr8.2:36582774-36582793 None:intergenic 35.0%
! ACCCATCATAGAATTTTCCA+TGG + chr8.2:36582684-36582703 None:intergenic 35.0%
! ACGATCTATTTTCTGAATCG+TGG - chr8.2:36583391-36583410 MS.gene009065:intron 35.0%
! AGTTTTCGGAGTTAGTAAGA+AGG + chr8.2:36582760-36582779 None:intergenic 35.0%
! GGTGGTTTTTGTGATGTTAA+TGG - chr8.2:36582782-36582801 MS.gene009065:CDS 35.0%
! TAGTGGTTCTATGATGAAGA+GGG - chr8.2:36582892-36582911 MS.gene009065:intron 35.0%
! TTCAACAACAAGTTGAGGTA+TGG - chr8.2:36584338-36584357 MS.gene009065:intron 35.0%
! TTTGAGGTAGATAGAAAGGA+GGG - chr8.2:36582599-36582618 MS.gene009065:CDS 35.0%
!! TCTCAGTTTTCAACTGATTC+TGG - chr8.2:36582959-36582978 MS.gene009065:CDS 35.0%
!! TGCAATGGATTAACCTGATT+TGG + chr8.2:36585273-36585292 None:intergenic 35.0%
!!! TTTTGAGGTAGATAGAAAGG+AGG - chr8.2:36582598-36582617 MS.gene009065:CDS 35.0%
AAATGTACCTTGTTGCATCC+AGG + chr8.2:36584172-36584191 None:intergenic 40.0%
AACATCTTACTCTCTCTGCA+AGG + chr8.2:36584024-36584043 None:intergenic 40.0%
AGAGTTGAATGAATCAGTCC+TGG + chr8.2:36585168-36585187 None:intergenic 40.0%
AGGGTTTAAAGAGCCAAATC+AGG - chr8.2:36585257-36585276 MS.gene009065:CDS 40.0%
ATAAACTCACATGGACAAGG+GGG + chr8.2:36583766-36583785 None:intergenic 40.0%
ATAGAACAGTTGGAATGGGT+TGG - chr8.2:36582861-36582880 MS.gene009065:intron 40.0%
ATGAACCTCAATAGAGTCGT+AGG - chr8.2:36583509-36583528 MS.gene009065:intron 40.0%
CAAATGTTGGATTGTGCTAG+TGG - chr8.2:36583241-36583260 MS.gene009065:intron 40.0%
CAACTGTGAGAGCAGAAAAA+AGG - chr8.2:36583865-36583884 MS.gene009065:CDS 40.0%
CACTAGCACAATCCAACATT+TGG + chr8.2:36583243-36583262 None:intergenic 40.0%
CAGATTCTTCAACATCAACC+GGG - chr8.2:36585072-36585091 MS.gene009065:CDS 40.0%
CCTGTAATGTCTCAAGATGA+AGG - chr8.2:36583151-36583170 MS.gene009065:intron 40.0%
CCTTCATCTTGAGACATTAC+AGG + chr8.2:36583154-36583173 None:intergenic 40.0%
CTGGATTTATTGATGCTGCA+AGG - chr8.2:36582978-36582997 MS.gene009065:intron 40.0%
CTTCTTCGATCAATCTGGAT+TGG + chr8.2:36583124-36583143 None:intergenic 40.0%
GGGGGAAACAGTATAAATCA+TGG + chr8.2:36583748-36583767 None:intergenic 40.0%
GGTTGTGATGATAGAACAGT+TGG - chr8.2:36582851-36582870 MS.gene009065:intron 40.0%
GTCTCTTCAACAACAAGTTG+AGG - chr8.2:36584333-36584352 MS.gene009065:intron 40.0%
GTTTCTCAGAGAAATGCCAA+TGG - chr8.2:36585113-36585132 MS.gene009065:CDS 40.0%
TATAGCACCAATAGCGCTAA+TGG + chr8.2:36584588-36584607 None:intergenic 40.0%
TCATCATAGAACCACTACCA+AGG + chr8.2:36582889-36582908 None:intergenic 40.0%
TGCAACAGTAAATCCACATG+TGG - chr8.2:36584925-36584944 MS.gene009065:CDS 40.0%
TGGATATGATGAACTCGTGT+GGG - chr8.2:36583029-36583048 MS.gene009065:intron 40.0%
TTGTCCTTTGTGGTTTCACT+TGG + chr8.2:36583848-36583867 None:intergenic 40.0%
TTGTTTCGCGATACACTTAG+AGG + chr8.2:36584539-36584558 None:intergenic 40.0%
! AGGGATGGTGTTTTACCAAA+TGG - chr8.2:36582911-36582930 MS.gene009065:CDS 40.0%
! ATCTGTAGGTTTGGTTTCCA+TGG - chr8.2:36582664-36582683 MS.gene009065:CDS 40.0%
! GAAGTGAGAAAAGATGGGTT+TGG - chr8.2:36582815-36582834 MS.gene009065:intron 40.0%
! GATGAATCAAGTGGTGATGA+TGG - chr8.2:36583214-36583233 MS.gene009065:intron 40.0%
! GGATGGTGTTTTACCAAATG+GGG - chr8.2:36582913-36582932 MS.gene009065:CDS 40.0%
! GGGATGGTGTTTTACCAAAT+GGG - chr8.2:36582912-36582931 MS.gene009065:CDS 40.0%
! GTAGTGGTTCTATGATGAAG+AGG - chr8.2:36582891-36582910 MS.gene009065:intron 40.0%
! TAGAACAGTTGGAATGGGTT+GGG - chr8.2:36582862-36582881 MS.gene009065:intron 40.0%
! TGACACATTTTTCTCCCTCT+TGG - chr8.2:36584882-36584901 MS.gene009065:CDS 40.0%
!! GATAATGGAGGAAAAGCCAT+TGG + chr8.2:36585132-36585151 None:intergenic 40.0%
!! TGATGATGGTTCCCAAATGT+TGG - chr8.2:36583228-36583247 MS.gene009065:intron 40.0%
!!! AAGATGGGTTTGGTTTTGCA+AGG - chr8.2:36582825-36582844 MS.gene009065:intron 40.0%
!!! AGATGGGTTTGGTTTTGCAA+GGG - chr8.2:36582826-36582845 MS.gene009065:intron 40.0%
!!! AGGATGTCATGTTTTAGTGC+CGG - chr8.2:36582998-36583017 MS.gene009065:intron 40.0%
AAGGGAAACAAGCTCTTGGA+GGG - chr8.2:36583170-36583189 MS.gene009065:intron 45.0%
ACATGTACGTATCCCTCGTT+AGG + chr8.2:36583965-36583984 None:intergenic 45.0%
ACGAGGGATACGTACATGTT+AGG - chr8.2:36583966-36583985 MS.gene009065:CDS 45.0%
ACTAACTCCGAAAACTTGGG+TGG - chr8.2:36582764-36582783 MS.gene009065:CDS 45.0%
AGAGGTAGAGCCATAGATTG+AGG + chr8.2:36583058-36583077 None:intergenic 45.0%
CATCATATCCACGAAACTCC+CGG + chr8.2:36583020-36583039 None:intergenic 45.0%
CGAGGGATACGTACATGTTA+GGG - chr8.2:36583967-36583986 MS.gene009065:CDS 45.0%
CTCTACCTCTACATGTGTCT+CGG - chr8.2:36583068-36583087 MS.gene009065:intron 45.0%
CTTCAACAACCAAGGCTTCT+GGG - chr8.2:36583903-36583922 MS.gene009065:CDS 45.0%
GAAAGGAGGGTCCTTTAGAT+TGG - chr8.2:36582612-36582631 MS.gene009065:CDS 45.0%
GATGAAGGGAAACAAGCTCT+TGG - chr8.2:36583166-36583185 MS.gene009065:intron 45.0%
GCAAACAGCTTCAACAACCA+AGG - chr8.2:36583895-36583914 MS.gene009065:CDS 45.0%
GCTACCAAGTGAAACCACAA+AGG - chr8.2:36583841-36583860 MS.gene009065:intron 45.0%
GCTGATGGAGATGAATCAAG+TGG - chr8.2:36583205-36583224 MS.gene009065:intron 45.0%
GGAAATATCTCAGCCCCATT+TGG + chr8.2:36582929-36582948 None:intergenic 45.0%
GGCTGCTTCCTAAAGATGTA+TGG - chr8.2:36584963-36584982 MS.gene009065:CDS 45.0%
GGTTCTATGATGAAGAGGGA+TGG - chr8.2:36582896-36582915 MS.gene009065:intron 45.0%
GTGGATATGATGAACTCGTG+TGG - chr8.2:36583028-36583047 MS.gene009065:intron 45.0%
GTGTGGGATTCCTCAATCTA+TGG - chr8.2:36583045-36583064 MS.gene009065:intron 45.0%
TAATCCATTGCAAGCAGCCA+AGG - chr8.2:36585281-36585300 MS.gene009065:CDS 45.0%
TCAACCACAGTTGGAACCTT+TGG - chr8.2:36585230-36585249 MS.gene009065:CDS 45.0%
TCTGGGAAGAATGCCAAACA+GGG - chr8.2:36583920-36583939 MS.gene009065:CDS 45.0%
TCTTGTACCTGGATGCAACA+AGG - chr8.2:36584162-36584181 MS.gene009065:intron 45.0%
TCTTTAAACCCTCCAGCCAA+AGG + chr8.2:36585249-36585268 None:intergenic 45.0%
TGCTATGCCATTAGCGCTAT+TGG - chr8.2:36584578-36584597 MS.gene009065:CDS 45.0%
TTACTCTCTCTGCAAGGCTA+TGG + chr8.2:36584018-36584037 None:intergenic 45.0%
TTGGACCTACGACTCTATTG+AGG + chr8.2:36583517-36583536 None:intergenic 45.0%
! ACTTGGTAGCTCAAGAGTTC+CGG + chr8.2:36583831-36583850 None:intergenic 45.0%
! GGCTATTTGTTGCTTGACCA+CGG + chr8.2:36583997-36584016 None:intergenic 45.0%
! GGGTCTTGTAATGAAGCTGA+TGG - chr8.2:36583190-36583209 MS.gene009065:intron 45.0%
! GGTGATAGTGATAAAGCCAC+CGG - chr8.2:36583809-36583828 MS.gene009065:intron 45.0%
! TTCTGGGAAGAATGCCAAAC+AGG - chr8.2:36583919-36583938 MS.gene009065:CDS 45.0%
!! GTTGAGCATCTTGGTAGTGA+TGG - chr8.2:36583094-36583113 MS.gene009065:intron 45.0%
!!! GGATGTCATGTTTTAGTGCC+GGG - chr8.2:36582999-36583018 MS.gene009065:intron 45.0%
!!! TTAAAAAATTCATATTTTAT+TGG + chr8.2:36583452-36583471 None:intergenic 5.0%
ACAGACCGAGACACATGTAG+AGG + chr8.2:36583076-36583095 None:intergenic 50.0%
AGAAACCCCAGAGCAGATGA+TGG + chr8.2:36585099-36585118 None:intergenic 50.0%
AGAAGCTTGAGACCCCTGTT+TGG + chr8.2:36583936-36583955 None:intergenic 50.0%
AGGAGGGTCCTTTAGATTGG+AGG - chr8.2:36582615-36582634 MS.gene009065:CDS 50.0%
AGTTGGAACCTTTGGCTGGA+GGG - chr8.2:36585238-36585257 MS.gene009065:CDS 50.0%
CTGGGAAGAATGCCAAACAG+GGG - chr8.2:36583921-36583940 MS.gene009065:CDS 50.0%
CTGTTTGTAGGTCACTGGCA+AGG - chr8.2:36584276-36584295 MS.gene009065:intron 50.0%
GAAGGGAAACAAGCTCTTGG+AGG - chr8.2:36583169-36583188 MS.gene009065:intron 50.0%
GCAGACTGTTCAACCACAGT+TGG - chr8.2:36585221-36585240 MS.gene009065:CDS 50.0%
GCTCTCACAGTTGTCCTTTG+TGG + chr8.2:36583858-36583877 None:intergenic 50.0%
GCTTCAACAACCAAGGCTTC+TGG - chr8.2:36583902-36583921 MS.gene009065:CDS 50.0%
TCTCGGTCTGTTGAGCATCT+TGG - chr8.2:36583085-36583104 MS.gene009065:intron 50.0%
TGTACGTATCCCTCGTTAGG+CGG + chr8.2:36583962-36583981 None:intergenic 50.0%
!! TTTTAGTGCCGGGAGTTTCG+TGG - chr8.2:36583009-36583028 MS.gene009065:intron 50.0%
!!! GGGTTTGGTTTTGCAAGGGT+TGG - chr8.2:36582830-36582849 MS.gene009065:intron 50.0%
AGCTTCTGATCCGCCTAACG+AGG - chr8.2:36583949-36583968 MS.gene009065:CDS 55.0%
CAGTCCTTGGCTGCTTGCAA+TGG + chr8.2:36585288-36585307 None:intergenic 55.0%
CAGTTGGAACCTTTGGCTGG+AGG - chr8.2:36585237-36585256 MS.gene009065:CDS 55.0%
CCACAGTTGGAACCTTTGGC+TGG - chr8.2:36585234-36585253 MS.gene009065:CDS 55.0%
CCAGCCAAAGGTTCCAACTG+TGG + chr8.2:36585237-36585256 None:intergenic 55.0%
GCTGTAGCGCTGTGGAATTG+CGG + chr8.2:36584485-36584504 None:intergenic 55.0%
GCTTCTGATCCGCCTAACGA+GGG - chr8.2:36583950-36583969 MS.gene009065:CDS 55.0%
GGCATTCTTCCCAGAAGCCT+TGG + chr8.2:36583915-36583934 None:intergenic 55.0%
TGGTAGCTCAAGAGTTCCGG+TGG + chr8.2:36583828-36583847 None:intergenic 55.0%
!! ATGGGTTGGGACCTTGGTAG+TGG - chr8.2:36582875-36582894 MS.gene009065:intron 55.0%
!! GTTGGAATGGGTTGGGACCT+TGG - chr8.2:36582869-36582888 MS.gene009065:intron 55.0%
ACCGGGTCCATCATCTGCTC+TGG - chr8.2:36585089-36585108 MS.gene009065:CDS 60.0%
AGCAGTGTGCTGTAGCGCTG+TGG + chr8.2:36584493-36584512 None:intergenic 60.0%
CACTGGCAAGGCAGTGATGC+TGG - chr8.2:36584288-36584307 MS.gene009065:intron 60.0%
CCGGGTCCATCATCTGCTCT+GGG - chr8.2:36585090-36585109 MS.gene009065:CDS 60.0%
CGGGTCCATCATCTGCTCTG+GGG - chr8.2:36585091-36585110 MS.gene009065:CDS 60.0%
GTACATGTTAGGGCTCGCCG+TGG - chr8.2:36583977-36583996 MS.gene009065:CDS 60.0%
!! CCCAGAGCAGATGATGGACC+CGG + chr8.2:36585093-36585112 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 36582581 36585308 36582581 ID=MS.gene009065
chr8.2 mRNA 36582581 36585308 36582581 ID=MS.gene009065.t1;Parent=MS.gene009065
chr8.2 exon 36584574 36585308 36584574 ID=MS.gene009065.t1.exon1;Parent=MS.gene009065.t1
chr8.2 CDS 36584574 36585308 36584574 ID=cds.MS.gene009065.t1;Parent=MS.gene009065.t1
chr8.2 exon 36583856 36584083 36583856 ID=MS.gene009065.t1.exon2;Parent=MS.gene009065.t1
chr8.2 CDS 36583856 36584083 36583856 ID=cds.MS.gene009065.t1;Parent=MS.gene009065.t1
chr8.2 exon 36583706 36583771 36583706 ID=MS.gene009065.t1.exon3;Parent=MS.gene009065.t1
chr8.2 CDS 36583706 36583771 36583706 ID=cds.MS.gene009065.t1;Parent=MS.gene009065.t1
chr8.2 exon 36583535 36583603 36583535 ID=MS.gene009065.t1.exon4;Parent=MS.gene009065.t1
chr8.2 CDS 36583535 36583603 36583535 ID=cds.MS.gene009065.t1;Parent=MS.gene009065.t1
chr8.2 exon 36582910 36582981 36582910 ID=MS.gene009065.t1.exon5;Parent=MS.gene009065.t1
chr8.2 CDS 36582910 36582981 36582910 ID=cds.MS.gene009065.t1;Parent=MS.gene009065.t1
chr8.2 exon 36582581 36582814 36582581 ID=MS.gene009065.t1.exon6;Parent=MS.gene009065.t1
chr8.2 CDS 36582581 36582814 36582581 ID=cds.MS.gene009065.t1;Parent=MS.gene009065.t1
Gene Sequence

>MS.gene009065

ATGAGTGAAAGTGAAAATTTTGAGGTAGATAGAAAGGAGGGTCCTTTAGATTGGAGGTTTATGAGTGGCAACCTTGCAAATTCATCTGTAGGTTTGGTTTCCATGGAAAATTCTATGATGGGTTCTTCTTCATGTCCTAATTCAATGGTAGATTCATATGGTCCTAATTTCTTGGACCTTCTTACTAACTCCGAAAACTTGGGTGGTTTTTGTGATGTTAATGGTCAGAGCAATGAAGTGAGAAAAGATGGGTTTGGTTTTGCAAGGGTTGGTTGTGATGATAGAACAGTTGGAATGGGTTGGGACCTTGGTAGTGGTTCTATGATGAAGAGGGATGGTGTTTTACCAAATGGGGCTGAGATATTTCCTCAAAGTTTGTCTCAGTTTTCAACTGATTCTGGATTTATTGATGCTGCAAGGATGTCATGTTTTAGTGCCGGGAGTTTCGTGGATATGATGAACTCGTGTGGGATTCCTCAATCTATGGCTCTACCTCTACATGTGTCTCGGTCTGTTGAGCATCTTGGTAGTGATGGAAGTCCAATCCAGATTGATCGAAGAAGTGATTGTCCTGTAATGTCTCAAGATGAAGGGAAACAAGCTCTTGGAGGGTCTTGTAATGAAGCTGATGGAGATGAATCAAGTGGTGATGATGGTTCCCAAATGTTGGATTGTGCTAGTGGAGAACCTTCTATTAAAGGTCTAAATCCAAAGAAAAGGAAAAGAAATGGACAGGTATATTTCTTTGAGTTGTATTTTTAGTATATTTGATACCGTGGTTATCAATTTGAGGTTTAAATCGTGAATCGGACGATCTATTTTCTGAATCGTGGATTGAATCTTTTATTATGAATCGTAAGATGAATTACCAATAAAATATGAATTTTTTAAGTAAAATCAAGCGACATAGCAAAATTATAAGCTGTAGATGAACCTCAATAGAGTCGTAGGTCCAAGTCATTAATCAAATGAAAAAAGGAAAGTGACTAGAGCTACACTAATTTTAGGTTGTGATTCATTAGAAACAATCAAAATTCATCATAGTGAATTGGGATTAAAAATGAGGTTCATATCCAAAATAGATACACTAGGGATTTGTATTGATCGAGTTTAGTTTTGTATCGAATCAAGATACAACTCACATGTATCGTAATATATTGATAACCATGATTTATACTGTTTCCCCCTTGTCCATGTGAGTTTATTTTATTTTATTTTCTGTTAGGATGGTGATAGTGATAAAGCCACCGGAACTCTTGAGCTACCAAGTGAAACCACAAAGGACAACTGTGAGAGCAGAAAAAAGGGAAAGCAGCAAACAGCTTCAACAACCAAGGCTTCTGGGAAGAATGCCAAACAGGGGTCTCAAGCTTCTGATCCGCCTAACGAGGGATACGTACATGTTAGGGCTCGCCGTGGTCAAGCAACAAATAGCCATAGCCTTGCAGAGAGAGTAAGATGTTCTAACTAAATTTATGTGATGAGAAAAAAAAATTGTTTGGATTTAGATTTCTAATTATTTAGATTTCAACTGTAGGTGAGGAGAGAAAAGATCAGTGAAAGAATGAAGTTTCTTCAAGATCTTGTACCTGGATGCAACAAGGTACATTTAAAACTTGAGACTGGAAGATTTCACTACCCTATATTCATATGAGGATTTAGAGTGTTGTTTTCTTTTGATATATCATTGTTATTCTGTTTGTAGGTCACTGGCAAGGCAGTGATGCTGGATGAAATCATTAATTACGTACAGTCTCTTCAACAACAAGTTGAGGTATGGATAAAAGCATATTTCTAACCACTGATTATTAAATCATCCAACTGATTTTCCCATATTTTTGTATTCTGCTTCAGTAAGTGTTGTCAATCATGAATCAGAGAAAATAACATTTGTTCAAATTCCGCAATTCCACAGCGCTACAGCACACTGCTATAGCTTCTATTTGATAACACTTCCTCTAAGTGTATCGCGAAACAATAGCAATTTGTTCAAATTCTGCTATGCCATTAGCGCTATTGGTGCTATAGCTGTTATTCGACAACATAGGCTTCAGTACGCTATCAGCATATGTTCCAGTAGAGTTTCTTATTGACATCGTTGACTGTTATAATGTTATTCAACGTGTTTGAAATTGCAATAATTTTAACTCTCACTGCTGATGATTTAAATGCCAGTGTCATCTTTGTTACAATTGGTAAATTATGACGAATAATTATAAGATAAACTGTTTTGTTAGGTGCCTTTGTTAGTTGTCCCTTATATATGTTTCTAGAAATCTATGCATCTTTTTCAGTTGAAGTTGACACATTTTTCTCCCTCTTGGCAGTTTTTATCAATGAAACTTGCAACAGTAAATCCACATGTGGATTTTAATATGGAACGGCTGCTTCCTAAAGATGTATGGTTCTAGTCAATTTGCATACCAGCAATTTTAAAGCTTACAATTAGCTATATATGCCTGACTGACCTTTATGAAATTGAATATTTTGTCAGATTCTTCAACATCAACCGGGTCCATCATCTGCTCTGGGGTTTCTCAGAGAAATGCCAATGGCTTTTCCTCCATTATCACATTCATATCAACCAGGACTGATTCATTCAACTCTACCTAACATGGCAAATTTCTCAGATATGCTTAGGCAGACTGTTCAACCACAGTTGGAACCTTTGGCTGGAGGGTTTAAAGAGCCAAATCAGGTTAATCCATTGCAAGCAGCCAAGGACTGA
Protein sequence

>MS.gene009065.t1

MSESENFEVDRKEGPLDWRFMSGNLANSSVGLVSMENSMMGSSSCPNSMVDSYGPNFLDLLTNSENLGGFCDVNGQSNEVRKDGFGFARVGCDDRTVGMGWDLGSGSMMKRDGVLPNGAEIFPQSLSQFSTDSGFIDAARMSCFSAGSFVDMMNSCGIPQSMALPLHVSRSVEHLGSDGSPIQIDRRSDCPVMSQDEGKQALGGSCNEADGDESSGDDGSQMLDCASGEPSIKGLNPKKRKRNGQDGDSDKATGTLELPSETTKDNCESRKKGKQQTASTTKASGKNAKQGSQASDPPNEGYVHVRARRGQATNSHSLAERVRREKISERMKFLQDLVPGCNKVTGKAVMLDEIINYVQSLQQQVEFLSMKLATVNPHVDFNMERLLPKDILQHQPGPSSALGFLREMPMAFPPLSHSYQPGLIHSTLPNMANFSDMLRQTVQPQLEPLAGGFKEPNQVNPLQAAKD