Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009114.t1 | KEH19993.1 | 95.2 | 63 | 3 | 0 | 78 | 140 | 10 | 72 | 3.20E-27 | 131.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009114.t1 | P93831 | 56.5 | 62 | 27 | 0 | 77 | 138 | 839 | 900 | 5.5e-12 | 72.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009114.t1 | A0A072TT25 | 95.2 | 63 | 3 | 0 | 78 | 140 | 10 | 72 | 2.3e-27 | 131.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene009114.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009114.t1 | MTR_8g064320 | 95.238 | 63 | 3 | 0 | 78 | 140 | 10 | 72 | 2.10e-40 | 132 |
| MS.gene009114.t1 | MTR_8g064250 | 89.062 | 64 | 7 | 0 | 77 | 140 | 7 | 70 | 2.02e-38 | 127 |
| MS.gene009114.t1 | MTR_7g109560 | 84.615 | 65 | 10 | 0 | 77 | 141 | 699 | 763 | 1.24e-33 | 125 |
| MS.gene009114.t1 | MTR_8g064310 | 83.607 | 61 | 10 | 0 | 77 | 137 | 7 | 67 | 3.78e-33 | 112 |
| MS.gene009114.t1 | MTR_8g064240 | 85.000 | 60 | 9 | 0 | 78 | 137 | 26 | 85 | 4.75e-33 | 113 |
| MS.gene009114.t1 | MTR_1g086980 | 59.091 | 66 | 24 | 2 | 77 | 142 | 769 | 831 | 2.14e-17 | 79.0 |
| MS.gene009114.t1 | MTR_1g086980 | 45.283 | 106 | 48 | 5 | 38 | 142 | 715 | 811 | 2.61e-17 | 78.6 |
| MS.gene009114.t1 | MTR_1g086980 | 59.091 | 66 | 24 | 2 | 77 | 142 | 759 | 821 | 2.66e-17 | 78.6 |
| MS.gene009114.t1 | MTR_1g086980 | 59.091 | 66 | 24 | 2 | 77 | 142 | 718 | 780 | 2.67e-17 | 78.6 |
| MS.gene009114.t1 | MTR_1g086980 | 59.091 | 66 | 24 | 2 | 77 | 142 | 697 | 759 | 2.84e-17 | 78.6 |
| MS.gene009114.t1 | MTR_1g086980 | 59.091 | 66 | 24 | 2 | 77 | 142 | 569 | 631 | 3.50e-17 | 78.2 |
| MS.gene009114.t1 | MTR_5g016870 | 54.839 | 62 | 27 | 1 | 77 | 138 | 801 | 861 | 1.01e-14 | 71.2 |
| MS.gene009114.t1 | MTR_7g055660 | 53.226 | 62 | 27 | 2 | 78 | 138 | 670 | 730 | 2.83e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009114.t1 | AT2G23380 | 56.452 | 62 | 27 | 0 | 77 | 138 | 839 | 900 | 3.07e-16 | 75.9 |
| MS.gene009114.t1 | AT2G23380 | 56.452 | 62 | 27 | 0 | 77 | 138 | 612 | 673 | 3.42e-16 | 75.5 |
| MS.gene009114.t1 | AT4G02020 | 53.030 | 66 | 28 | 2 | 77 | 142 | 794 | 856 | 1.02e-13 | 68.6 |
Find 39 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCTCACTTGAGGAAATT+TGG | 0.131738 | 8.1:-39280495 | MS.gene009114:CDS |
| CAAGTGAGAGCAATGTTTCT+TGG | 0.232152 | 8.1:+39280506 | None:intergenic |
| CAGAATTCAGCTCAGCAATT+TGG | 0.247609 | 8.1:+39281720 | None:intergenic |
| GAAGCTTGAGGAAGAATTAA+TGG | 0.278658 | 8.1:-39281753 | MS.gene009114:CDS |
| TTGTAGGTTGTGCTTGTTAA+TGG | 0.284481 | 8.1:-39280690 | MS.gene009114:intron |
| TTGTTGTACTGCAGTGTCTT+TGG | 0.325940 | 8.1:+39281694 | None:intergenic |
| TGTAGGTTGTGCTTGTTAAT+GGG | 0.345826 | 8.1:-39280689 | MS.gene009114:intron |
| CAAAGGAACGTATTGAAGTT+GGG | 0.371751 | 8.1:-39280644 | MS.gene009114:CDS |
| GCAAAGGAACGTATTGAAGT+TGG | 0.419225 | 8.1:-39280645 | MS.gene009114:CDS |
| CCTGTCTGTGGAACACCCTT+TGG | 0.422322 | 8.1:-39280453 | MS.gene009114:CDS |
| CCTGTCTGTGGAACACCCTT+TGG | 0.422322 | 8.1:+39281580 | None:intergenic |
| CTTCATAAACTTATAGATGA+TGG | 0.430748 | 8.1:-39280570 | MS.gene009114:CDS |
| TGTTTGATCATCATCTTCCT+TGG | 0.431875 | 8.1:+39280545 | None:intergenic |
| CCTTTGGACAGAGTGATGTT+CGG | 0.489650 | 8.1:-39280437 | MS.gene009114:CDS |
| TTATGTCATGCAGGAGTTCA+CGG | 0.512323 | 8.1:-39281778 | MS.gene009114:CDS |
| CCGAGTCATCACTCATGTAA+TGG | 0.524250 | 8.1:+39281636 | None:intergenic |
| CTCATCATATTATGTCATGC+AGG | 0.524762 | 8.1:-39281787 | None:intergenic |
| TTCCACAGACAGGACATTTG+CGG | 0.530952 | 8.1:+39280463 | None:intergenic |
| TTCCACAGACAGGACATTTG+CGG | 0.530952 | 8.1:-39281570 | MS.gene009114:intron |
| CGAACATCACTCTGTCCAAA+GGG | 0.531423 | 8.1:-39281595 | MS.gene009114:CDS |
| CCAAAGGGTGTTCCACAGAC+AGG | 0.536944 | 8.1:+39280453 | None:intergenic |
| CCAAAGGGTGTTCCACAGAC+AGG | 0.536944 | 8.1:-39281580 | MS.gene009114:CDS |
| CGAACATCACTCTGTCCAAA+GGG | 0.542541 | 8.1:+39280438 | None:intergenic |
| AGAAACATTGCTCTCACTTG+AGG | 0.546978 | 8.1:-39280503 | MS.gene009114:CDS |
| GTAGGTTGTGCTTGTTAATG+GGG | 0.554178 | 8.1:-39280688 | MS.gene009114:intron |
| CCATTACATGAGTGATGACT+CGG | 0.557579 | 8.1:-39281636 | MS.gene009114:CDS |
| CCGAACATCACTCTGTCCAA+AGG | 0.571995 | 8.1:+39280437 | None:intergenic |
| ACGAACATCACTCTGTCCAA+AGG | 0.573084 | 8.1:-39281596 | MS.gene009114:CDS |
| ACTTATAGATGATGGTTCCA+AGG | 0.573120 | 8.1:-39280562 | MS.gene009114:CDS |
| TCTATAAGTTTATGAAGCCA+TGG | 0.576047 | 8.1:+39280576 | None:intergenic |
| AAAGGAACGTATTGAAGTTG+GGG | 0.584434 | 8.1:-39280643 | MS.gene009114:CDS |
| CACCGCAAATGTCCTGTCTG+TGG | 0.604351 | 8.1:+39281568 | None:intergenic |
| CACCGCAAATGTCCTGTCTG+TGG | 0.606304 | 8.1:-39280465 | MS.gene009114:CDS |
| GTTAATGGGGATCATCGAGT+AGG | 0.622578 | 8.1:-39280675 | MS.gene009114:CDS |
| TCGAGTAGGAATATTTGCAA+AGG | 0.624767 | 8.1:-39280661 | MS.gene009114:CDS |
| GAGTTCACGGAAGAAGCTTG+AGG | 0.633563 | 8.1:-39281765 | MS.gene009114:CDS |
| GGAACGTATTGAAGTTGGGG+AGG | 0.649243 | 8.1:-39280640 | MS.gene009114:CDS |
| ATGAAAATAGTCGACCACCA+TGG | 0.704208 | 8.1:-39280593 | MS.gene009114:CDS |
| ATAAGTTTATGAAGCCATGG+TGG | 0.735249 | 8.1:+39280579 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAACATAATATATGTTTTTA+GGG | - | chr8.1:39281091-39281110 | MS.gene009114:intron | 10.0% |
| !!! | TAACATAATATATGTTTTTA+GGG | + | chr8.1:39281110-39281091 | None:intergenic | 10.0% |
| !!! | TTAACATAATATATGTTTTT+AGG | - | chr8.1:39281090-39281109 | MS.gene009114:intron | 10.0% |
| !!! | TTAACATAATATATGTTTTT+AGG | + | chr8.1:39281109-39281090 | None:intergenic | 10.0% |
| !! | CTTCATAAACTTCTATATAA+TGG | + | chr8.1:39280738-39280757 | None:intergenic | 20.0% |
| !! | TAGATAGTAAAAGACTATAT+AGG | + | chr8.1:39281205-39281224 | None:intergenic | 20.0% |
| !!! | TATATGTTTTTAGGGATATA+AGG | - | chr8.1:39281099-39281118 | MS.gene009114:intron | 20.0% |
| !!! | TATATGTTTTTAGGGATATA+AGG | + | chr8.1:39281118-39281099 | None:intergenic | 20.0% |
| ! | AAAAGCTAATTGATATGCAA+AGG | - | chr8.1:39281380-39281399 | MS.gene009114:intron | 25.0% |
| ! | AGAAAAAAATCATAGTAAGC+TGG | - | chr8.1:39281066-39281085 | MS.gene009114:intron | 25.0% |
| ! | CTGAAGAAAAAATCATAGTA+AGG | + | chr8.1:39281304-39281323 | None:intergenic | 25.0% |
| ! | CTTCATAAACTTATAGATGA+TGG | - | chr8.1:39281628-39281647 | MS.gene009114:CDS | 25.0% |
| AAAAGCTAATTGCTATGCAA+AGG | + | chr8.1:39280986-39281005 | None:intergenic | 30.0% | |
| AAAGGAACATATTGAAGCAA+GGG | + | chr8.1:39280811-39280830 | None:intergenic | 30.0% | |
| AATAATGTGTCTCCTATTGT+AGG | + | chr8.1:39280874-39280893 | None:intergenic | 30.0% | |
| AATAATGTGTGTCCTATTGT+AGG | - | chr8.1:39281492-39281511 | MS.gene009114:intron | 30.0% | |
| ACTTCTATATAATGGTTCCA+AGG | + | chr8.1:39280730-39280749 | None:intergenic | 30.0% | |
| TATAGAAGTTTATGAAGCCA+TGG | - | chr8.1:39280741-39280760 | MS.gene009114:intron | 30.0% | |
| TCTATAAGTTTATGAAGCCA+TGG | + | chr8.1:39281625-39281644 | None:intergenic | 30.0% | |
| ! | GATGATCAAACATTTTCTCA+AGG | - | chr8.1:39281664-39281683 | MS.gene009114:CDS | 30.0% |
| AAAGGAACGTATTGAAGTTG+GGG | - | chr8.1:39281555-39281574 | MS.gene009114:intron | 35.0% | |
| ATAAGTTTATGAAGCCATGG+TGG | + | chr8.1:39281622-39281641 | None:intergenic | 35.0% | |
| CAAAGGAACATATTGAAGCA+AGG | + | chr8.1:39280812-39280831 | None:intergenic | 35.0% | |
| CAAAGGAACGTATTGAAGTT+GGG | - | chr8.1:39281554-39281573 | MS.gene009114:intron | 35.0% | |
| GAAGCTTGAGGAAGAATTAA+TGG | - | chr8.1:39280445-39280464 | MS.gene009114:CDS | 35.0% | |
| GATTACGGGTATGATGAAAA+TGG | + | chr8.1:39281611-39281592 | None:intergenic | 35.0% | |
| GGATGACTTAATGCACAAAA+AGG | + | chr8.1:39281250-39281269 | None:intergenic | 35.0% | |
| GGATGACTTAATGCATGAAA+AGG | - | chr8.1:39281120-39281139 | MS.gene009114:intron | 35.0% | |
| TAACAAGCACAACCTACAAT+AGG | - | chr8.1:39280859-39280878 | MS.gene009114:intron | 35.0% | |
| TAACAAGCACAACCTACAAT+AGG | + | chr8.1:39280878-39280859 | None:intergenic | 35.0% | |
| TCGAGTAGGAATATTTGCAA+AGG | - | chr8.1:39281537-39281556 | MS.gene009114:intron | 35.0% | |
| TGTTTGATCATCATCTTCCT+TGG | + | chr8.1:39281656-39281675 | None:intergenic | 35.0% | |
| ! | ACTTATAGATGATGGTTCCA+AGG | - | chr8.1:39281636-39281655 | MS.gene009114:CDS | 35.0% |
| ! | AGGAGCTTTTCTTTGATTAC+GGG | - | chr8.1:39281578-39281597 | MS.gene009114:CDS | 35.0% |
| ! | AGGAGCTTTTCTTTGATTAC+GGG | + | chr8.1:39281597-39281578 | None:intergenic | 35.0% |
| ! | GAGGAGCTTTTCTTTGATTA+CGG | - | chr8.1:39281577-39281596 | MS.gene009114:CDS | 35.0% |
| ! | GAGGAGCTTTTCTTTGATTA+CGG | + | chr8.1:39281596-39281577 | None:intergenic | 35.0% |
| ! | GATGATCCAACATTTTCTCA+AGG | + | chr8.1:39280702-39280721 | None:intergenic | 35.0% |
| ! | TCCAACATTTTCTCAAGGAA+AGG | + | chr8.1:39280697-39280716 | None:intergenic | 35.0% |
| ! | TGTAGGTTGTGCTTGTTAAT+GGG | - | chr8.1:39281509-39281528 | MS.gene009114:intron | 35.0% |
| ! | TGTAGGTTGTGCTTGTTAAT+GGG | + | chr8.1:39281528-39281509 | None:intergenic | 35.0% |
| ! | TTGTAGGTTGTGCTTGTTAA+TGG | - | chr8.1:39281508-39281527 | MS.gene009114:intron | 35.0% |
| ! | TTGTAGGTTGTGCTTGTTAA+TGG | + | chr8.1:39281527-39281508 | None:intergenic | 35.0% |
| !! | TAGAGTAGGAGTATTTGCAA+AGG | + | chr8.1:39280829-39280848 | None:intergenic | 35.0% |
| AGAAACATTGCTCTCACTTG+AGG | - | chr8.1:39281695-39281714 | MS.gene009114:CDS | 40.0% | |
| ATGAAAATAGTCGACCACCA+TGG | - | chr8.1:39281605-39281624 | MS.gene009114:CDS | 40.0% | |
| ATGAAAATGGTCGATCACCA+TGG | + | chr8.1:39280761-39280780 | None:intergenic | 40.0% | |
| CAAGTGAGAGCAATGTTTCT+TGG | + | chr8.1:39281695-39281714 | None:intergenic | 40.0% | |
| CAGAATTCAGCTCAGCAATT+TGG | + | chr8.1:39280481-39280500 | None:intergenic | 40.0% | |
| CCATTACATGAGTGATGACT+CGG | - | chr8.1:39280562-39280581 | MS.gene009114:CDS | 40.0% | |
| GCAAAGGAACGTATTGAAGT+TGG | - | chr8.1:39281553-39281572 | MS.gene009114:intron | 40.0% | |
| GCCTTTCCTTGAGAAAATGT+TGG | - | chr8.1:39280693-39280712 | MS.gene009114:intron | 40.0% | |
| GTCTTGATGCTTATCGCAAA+GGG | - | chr8.1:39281328-39281347 | MS.gene009114:intron | 40.0% | |
| GTTAATGGGGATCATAGAGT+AGG | + | chr8.1:39280843-39280862 | None:intergenic | 40.0% | |
| TGCTCTCACTTGAGGAAATT+TGG | - | chr8.1:39281703-39281722 | MS.gene009114:CDS | 40.0% | |
| TGTCTTGATGCTTATCGCAA+AGG | - | chr8.1:39281327-39281346 | MS.gene009114:intron | 40.0% | |
| TGTTGGATCATCATCTTCCT+TGG | - | chr8.1:39280710-39280729 | MS.gene009114:intron | 40.0% | |
| TTCTTGATGCTTCTCGCAAA+GGG | + | chr8.1:39281038-39281057 | None:intergenic | 40.0% | |
| TTGTTGTACTGCAGTGTCTT+TGG | + | chr8.1:39280507-39280526 | None:intergenic | 40.0% | |
| TTTCTTGATGCTTCTCGCAA+AGG | + | chr8.1:39281039-39281058 | None:intergenic | 40.0% | |
| ! | GTAGGTTGTGCTTGTTAATG+GGG | - | chr8.1:39281510-39281529 | MS.gene009114:intron | 40.0% |
| ! | GTAGGTTGTGCTTGTTAATG+GGG | + | chr8.1:39281529-39281510 | None:intergenic | 40.0% |
| ACGAACATCACTCTGTCCAA+AGG | - | chr8.1:39280602-39280621 | MS.gene009114:CDS | 45.0% | |
| AGAAACATTGCTCTCGCTTG+AGG | + | chr8.1:39280671-39280690 | None:intergenic | 45.0% | |
| CAAGCGAGAGCAATGTTTCT+TGG | - | chr8.1:39280671-39280690 | MS.gene009114:CDS | 45.0% | |
| CCGAGTCATCACTCATGTAA+TGG | + | chr8.1:39280565-39280584 | None:intergenic | 45.0% | |
| CCTTTGGACAGAGTGATGTT+CGG | - | chr8.1:39281761-39281780 | MS.gene009114:CDS | 45.0% | |
| CGAACATCACTCTGTCCAAA+GGG | - | chr8.1:39280603-39280622 | MS.gene009114:CDS | 45.0% | |
| CGAACATCACTCTGTCCAAA+GGG | + | chr8.1:39280622-39280603 | None:intergenic | 45.0% | |
| GGAACATATTGAAGCAAGGG+AGG | + | chr8.1:39280808-39280827 | None:intergenic | 45.0% | |
| GTTAATGGGGATCATCGAGT+AGG | - | chr8.1:39281523-39281542 | MS.gene009114:intron | 45.0% | |
| TGCTATGCAAAGGAGAGTCT+AGG | + | chr8.1:39280976-39280995 | None:intergenic | 45.0% | |
| TGCTCTCGCTTGAGGAAATT+TGG | + | chr8.1:39280663-39280682 | None:intergenic | 45.0% | |
| TTCCACAGACAGGACATTTG+CGG | - | chr8.1:39280628-39280647 | MS.gene009114:CDS | 45.0% | |
| TTCCACAGACAGGACATTTG+CGG | + | chr8.1:39280647-39280628 | None:intergenic | 45.0% | |
| CCGAACATCACTCTGTCCAA+AGG | + | chr8.1:39281764-39281783 | None:intergenic | 50.0% | |
| GAGTTCACGGAAGAAGCTTG+AGG | - | chr8.1:39280433-39280452 | MS.gene009114:CDS | 50.0% | |
| GGAACGTATTGAAGTTGGGG+AGG | - | chr8.1:39281558-39281577 | MS.gene009114:intron | 50.0% | |
| CACCGCAAATGTCCTGTCTG+TGG | - | chr8.1:39281733-39281752 | MS.gene009114:CDS | 55.0% | |
| CACCGCAAATGTCCTGTCTG+TGG | + | chr8.1:39281752-39281733 | None:intergenic | 55.0% | |
| CCTGTCTGTGGAACACCCTT+TGG | - | chr8.1:39281745-39281764 | MS.gene009114:CDS | 55.0% | |
| CCTGTCTGTGGAACACCCTT+TGG | + | chr8.1:39281764-39281745 | None:intergenic | 55.0% | |
| !! | CCAAAGGGTGTTCCACAGAC+AGG | - | chr8.1:39280618-39280637 | MS.gene009114:CDS | 55.0% |
| !! | CCAAAGGGTGTTCCACAGAC+AGG | + | chr8.1:39280637-39280618 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 39280422 | 39281798 | 39280422 | ID=MS.gene009114 |
| chr8.1 | mRNA | 39280422 | 39281798 | 39280422 | ID=MS.gene009114.t1;Parent=MS.gene009114 |
| chr8.1 | exon | 39281571 | 39281798 | 39281571 | ID=MS.gene009114.t1.exon1;Parent=MS.gene009114.t1 |
| chr8.1 | CDS | 39281571 | 39281798 | 39281571 | ID=cds.MS.gene009114.t1;Parent=MS.gene009114.t1 |
| chr8.1 | exon | 39280422 | 39280706 | 39280422 | ID=MS.gene009114.t1.exon2;Parent=MS.gene009114.t1 |
| chr8.1 | CDS | 39280422 | 39280706 | 39280422 | ID=cds.MS.gene009114.t1;Parent=MS.gene009114.t1 |
| Gene Sequence |
| Protein sequence |
