Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009157.t1 | XP_003628696.1 | 93.8 | 144 | 9 | 0 | 1 | 144 | 1 | 144 | 1.60E-67 | 265.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009157.t1 | F4JZT3 | 56.8 | 118 | 49 | 1 | 10 | 127 | 16 | 131 | 3.0e-27 | 122.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009157.t1 | G7LG58 | 93.8 | 144 | 9 | 0 | 1 | 144 | 1 | 144 | 1.1e-67 | 265.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene009157.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009157.t1 | MTR_8g063580 | 93.750 | 144 | 9 | 0 | 1 | 144 | 1 | 144 | 9.59e-98 | 277 |
| MS.gene009157.t1 | MTR_7g099500 | 68.182 | 132 | 36 | 3 | 1 | 132 | 1 | 126 | 2.68e-54 | 167 |
| MS.gene009157.t1 | MTR_7g098910 | 58.400 | 125 | 49 | 1 | 5 | 129 | 15 | 136 | 2.14e-42 | 137 |
| MS.gene009157.t1 | MTR_6g008860 | 34.259 | 108 | 69 | 1 | 12 | 119 | 33 | 138 | 2.62e-14 | 66.2 |
| MS.gene009157.t1 | MTR_4g071650 | 34.259 | 108 | 69 | 1 | 12 | 119 | 33 | 138 | 2.62e-14 | 66.2 |
| MS.gene009157.t1 | MTR_0022s0450 | 54.098 | 61 | 28 | 0 | 18 | 78 | 40 | 100 | 2.94e-14 | 64.7 |
| MS.gene009157.t1 | MTR_8g075340 | 31.915 | 141 | 73 | 4 | 12 | 131 | 1029 | 1167 | 1.52e-13 | 67.0 |
| MS.gene009157.t1 | MTR_8g106150 | 31.159 | 138 | 67 | 4 | 15 | 132 | 864 | 993 | 4.42e-13 | 65.5 |
| MS.gene009157.t1 | MTR_8g106150 | 31.159 | 138 | 67 | 4 | 15 | 132 | 621 | 750 | 4.68e-13 | 65.5 |
| MS.gene009157.t1 | MTR_7g098860 | 45.122 | 82 | 42 | 2 | 31 | 111 | 8 | 87 | 4.17e-12 | 58.9 |
| MS.gene009157.t1 | MTR_1g087140 | 38.017 | 121 | 55 | 5 | 15 | 122 | 976 | 1089 | 1.27e-11 | 61.6 |
| MS.gene009157.t1 | MTR_5g097410 | 41.791 | 67 | 39 | 0 | 15 | 81 | 1077 | 1143 | 2.93e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009157.t1 | AT5G26594 | 56.780 | 118 | 49 | 1 | 10 | 127 | 16 | 131 | 9.93e-38 | 125 |
| MS.gene009157.t1 | AT3G04280 | 50.000 | 116 | 56 | 2 | 12 | 126 | 22 | 136 | 2.18e-31 | 109 |
| MS.gene009157.t1 | AT3G04280 | 50.000 | 116 | 56 | 2 | 12 | 126 | 22 | 136 | 2.18e-31 | 109 |
| MS.gene009157.t1 | AT3G04280 | 50.000 | 116 | 56 | 2 | 12 | 126 | 22 | 136 | 2.18e-31 | 109 |
| MS.gene009157.t1 | AT2G17820 | 27.329 | 161 | 79 | 3 | 15 | 139 | 1047 | 1205 | 1.55e-12 | 64.3 |
| MS.gene009157.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 937 | 1052 | 5.40e-12 | 62.4 |
| MS.gene009157.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 948 | 1063 | 5.56e-12 | 62.4 |
| MS.gene009157.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.60e-12 | 62.4 |
| MS.gene009157.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.60e-12 | 62.4 |
| MS.gene009157.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.60e-12 | 62.4 |
| MS.gene009157.t1 | AT2G01830 | 30.579 | 121 | 66 | 3 | 15 | 122 | 925 | 1040 | 5.60e-12 | 62.4 |
Find 27 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAATAGTGATAAAATTTA+TGG | 0.245050 | 8.1:+39984299 | MS.gene009157:CDS |
| TGGTGTATCCTCACACTCTA+TGG | 0.342570 | 8.1:+39984269 | MS.gene009157:CDS |
| TGACCTAATCTTGATGGATA+AGG | 0.347722 | 8.1:+39983992 | MS.gene009157:CDS |
| ACGTCATTTGATGAATTCTT+CGG | 0.351042 | 8.1:-39983890 | None:intergenic |
| CACAACAAGTGCTGTTAACT+TGG | 0.355344 | 8.1:-39983850 | None:intergenic |
| CGTCATTTGATGAATTCTTC+GGG | 0.366651 | 8.1:-39983889 | None:intergenic |
| AACAAAGCAACTAAGGTTGA+TGG | 0.449452 | 8.1:+39984227 | MS.gene009157:CDS |
| GTGATAAAATTTATGGAAGC+AGG | 0.456780 | 8.1:+39984306 | MS.gene009157:CDS |
| AATTCCATTCTTCATGCCGT+TGG | 0.460947 | 8.1:+39984372 | MS.gene009157:CDS |
| TATCCTTATCCATCAAGATT+AGG | 0.462938 | 8.1:-39983995 | None:intergenic |
| TCTTGAGAAACCATTGGATA+AGG | 0.467024 | 8.1:+39984341 | MS.gene009157:CDS |
| GGGATATCTAGCACTATTGT+TGG | 0.469032 | 8.1:+39984249 | MS.gene009157:CDS |
| TATAGGCAACAAAGCAACTA+AGG | 0.474253 | 8.1:+39984220 | MS.gene009157:intron |
| TCATTTCATCTTGTGAGGAA+TGG | 0.476235 | 8.1:-39984418 | None:intergenic |
| CAAATGACGTTGAGTAACTT+AGG | 0.479404 | 8.1:+39983903 | MS.gene009157:CDS |
| ACCTCAGCTCCATTCATCTC+AGG | 0.491878 | 8.1:-39984020 | None:intergenic |
| AAGGATATGCCTGAGATGAA+TGG | 0.499108 | 8.1:+39984011 | MS.gene009157:CDS |
| ACAAAGCAACTAAGGTTGAT+GGG | 0.523464 | 8.1:+39984228 | MS.gene009157:CDS |
| CGAATATCTTGAGAAACCAT+TGG | 0.533361 | 8.1:+39984335 | MS.gene009157:CDS |
| TTTGTTCATTTCATCTTGTG+AGG | 0.533712 | 8.1:-39984423 | None:intergenic |
| CTCCTCTTCCATAGAGTGTG+AGG | 0.555361 | 8.1:-39984277 | None:intergenic |
| ATCCTCACACTCTATGGAAG+AGG | 0.570487 | 8.1:+39984275 | MS.gene009157:CDS |
| CAAGTTAACAGCACTTGTTG+TGG | 0.584291 | 8.1:+39983851 | MS.gene009157:CDS |
| CTCACACTCTATGGAAGAGG+AGG | 0.604784 | 8.1:+39984278 | MS.gene009157:CDS |
| CAAAGCAACTAAGGTTGATG+GGG | 0.617733 | 8.1:+39984229 | MS.gene009157:CDS |
| GCAGTGGAAATTCATCAGCA+TGG | 0.649906 | 8.1:+39983960 | MS.gene009157:CDS |
| GCCTGAGATGAATGGAGCTG+AGG | 0.680215 | 8.1:+39984019 | MS.gene009157:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTATGTGTTTCTTATAT+AGG | + | chr8.1:39984203-39984222 | MS.gene009157:intron | 15.0% |
| !!! | TTTTTCTTTTATTTTTCCAA+CGG | - | chr8.1:39984391-39984410 | None:intergenic | 15.0% |
| !! | GGAAATAGTGATAAAATTTA+TGG | + | chr8.1:39984299-39984318 | MS.gene009157:CDS | 20.0% |
| !! | AATTTTTGACCTAATCTTGA+TGG | + | chr8.1:39983986-39984005 | MS.gene009157:CDS | 25.0% |
| AACAACACTTGTGTGAAAAA+CGG | + | chr8.1:39983930-39983949 | MS.gene009157:CDS | 30.0% | |
| ACGTCATTTGATGAATTCTT+CGG | - | chr8.1:39983893-39983912 | None:intergenic | 30.0% | |
| GAGTTCATTCTTGCTTATTT+AGG | + | chr8.1:39984101-39984120 | MS.gene009157:intron | 30.0% | |
| GTGATAAAATTTATGGAAGC+AGG | + | chr8.1:39984306-39984325 | MS.gene009157:CDS | 30.0% | |
| TATCCTTATCCATCAAGATT+AGG | - | chr8.1:39983998-39984017 | None:intergenic | 30.0% | |
| !!! | TTGGATGTAAAGTTTTGAGA+TGG | - | chr8.1:39983834-39983853 | None:intergenic | 30.0% |
| AACAAAGCAACTAAGGTTGA+TGG | + | chr8.1:39984227-39984246 | MS.gene009157:CDS | 35.0% | |
| CAAATGACGTTGAGTAACTT+AGG | + | chr8.1:39983903-39983922 | MS.gene009157:CDS | 35.0% | |
| CGAATATCTTGAGAAACCAT+TGG | + | chr8.1:39984335-39984354 | MS.gene009157:CDS | 35.0% | |
| CGTCATTTGATGAATTCTTC+GGG | - | chr8.1:39983892-39983911 | None:intergenic | 35.0% | |
| TATAGGCAACAAAGCAACTA+AGG | + | chr8.1:39984220-39984239 | MS.gene009157:intron | 35.0% | |
| TCATTTCATCTTGTGAGGAA+TGG | - | chr8.1:39984421-39984440 | None:intergenic | 35.0% | |
| TCTTGAGAAACCATTGGATA+AGG | + | chr8.1:39984341-39984360 | MS.gene009157:CDS | 35.0% | |
| ! | ACAAAGCAACTAAGGTTGAT+GGG | + | chr8.1:39984228-39984247 | MS.gene009157:CDS | 35.0% |
| ! | TGACCTAATCTTGATGGATA+AGG | + | chr8.1:39983992-39984011 | MS.gene009157:CDS | 35.0% |
| ! | TTGAGTTTTGCCTTATCCAA+TGG | - | chr8.1:39984354-39984373 | None:intergenic | 35.0% |
| AAGGATATGCCTGAGATGAA+TGG | + | chr8.1:39984011-39984030 | MS.gene009157:CDS | 40.0% | |
| AATTCCATTCTTCATGCCGT+TGG | + | chr8.1:39984372-39984391 | MS.gene009157:CDS | 40.0% | |
| CATTCTTGCTTATTTAGGCG+TGG | + | chr8.1:39984106-39984125 | MS.gene009157:intron | 40.0% | |
| GAAAAACGGAAAAGAAGCAG+TGG | + | chr8.1:39983944-39983963 | MS.gene009157:CDS | 40.0% | |
| GGGATATCTAGCACTATTGT+TGG | + | chr8.1:39984249-39984268 | MS.gene009157:CDS | 40.0% | |
| ! | CAAGTTAACAGCACTTGTTG+TGG | + | chr8.1:39983851-39983870 | MS.gene009157:CDS | 40.0% |
| ! | CACAACAAGTGCTGTTAACT+TGG | - | chr8.1:39983853-39983872 | None:intergenic | 40.0% |
| ! | TTTTCCAACGGCATGAAGAA+TGG | - | chr8.1:39984379-39984398 | None:intergenic | 40.0% |
| !! | CAAAGCAACTAAGGTTGATG+GGG | + | chr8.1:39984229-39984248 | MS.gene009157:CDS | 40.0% |
| ATCCTCACACTCTATGGAAG+AGG | + | chr8.1:39984275-39984294 | MS.gene009157:CDS | 45.0% | |
| GCAGTGGAAATTCATCAGCA+TGG | + | chr8.1:39983960-39983979 | MS.gene009157:CDS | 45.0% | |
| TGGTGTATCCTCACACTCTA+TGG | + | chr8.1:39984269-39984288 | MS.gene009157:CDS | 45.0% | |
| !! | ATATATAAATGTAAAATTTA+GGG | - | chr8.1:39984065-39984084 | None:intergenic | 5.0% |
| !! | TATATATAAATGTAAAATTT+AGG | - | chr8.1:39984066-39984085 | None:intergenic | 5.0% |
| ACCTCAGCTCCATTCATCTC+AGG | - | chr8.1:39984023-39984042 | None:intergenic | 50.0% | |
| CTCACACTCTATGGAAGAGG+AGG | + | chr8.1:39984278-39984297 | MS.gene009157:CDS | 50.0% | |
| CTCCTCTTCCATAGAGTGTG+AGG | - | chr8.1:39984280-39984299 | None:intergenic | 50.0% | |
| GCCTGAGATGAATGGAGCTG+AGG | + | chr8.1:39984019-39984038 | MS.gene009157:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 39983822 | 39984440 | 39983822 | ID=MS.gene009157 |
| chr8.1 | mRNA | 39983822 | 39984440 | 39983822 | ID=MS.gene009157.t1;Parent=MS.gene009157 |
| chr8.1 | exon | 39983822 | 39984040 | 39983822 | ID=MS.gene009157.t1.exon1;Parent=MS.gene009157.t1 |
| chr8.1 | CDS | 39983822 | 39984040 | 39983822 | ID=cds.MS.gene009157.t1;Parent=MS.gene009157.t1 |
| chr8.1 | exon | 39984225 | 39984440 | 39984225 | ID=MS.gene009157.t1.exon2;Parent=MS.gene009157.t1 |
| chr8.1 | CDS | 39984225 | 39984440 | 39984225 | ID=cds.MS.gene009157.t1;Parent=MS.gene009157.t1 |
| Gene Sequence |
| Protein sequence |
