AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009272


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009272.t1 MTR_3g005480 86.192 239 23 1 22 260 36 264 3.14e-154 430
MS.gene009272.t1 MTR_3g005490 79.253 241 39 2 22 262 36 265 8.45e-138 389
MS.gene009272.t1 MTR_5g070440 70.954 241 68 1 22 262 36 274 2.94e-124 355
MS.gene009272.t1 MTR_3g005420 66.805 241 78 1 22 262 304 542 1.09e-110 330
MS.gene009272.t1 MTR_3g005420 55.983 234 77 4 22 253 55 264 2.07e-73 233
MS.gene009272.t1 MTR_3g005460 63.968 247 83 2 22 262 277 523 3.47e-105 315
MS.gene009272.t1 MTR_3g005460 56.769 229 74 3 22 250 40 243 3.18e-78 245
MS.gene009272.t1 MTR_3g005420 55.602 241 70 2 22 262 285 488 9.11e-83 256
MS.gene009272.t1 MTR_3g005420 55.983 234 77 4 22 253 36 245 3.74e-74 234
MS.gene009272.t1 MTR_3g005440 63.731 193 63 1 22 214 257 442 8.51e-77 239
MS.gene009272.t1 MTR_3g005440 49.580 238 67 3 18 253 31 217 1.47e-62 202
MS.gene009272.t1 MTR_3g005495 53.086 243 81 4 18 259 24 234 2.28e-76 239
MS.gene009272.t1 MTR_3g005495 52.766 235 90 6 22 254 258 473 8.05e-71 225
MS.gene009272.t1 MTR_1g015350 40.984 183 34 4 50 232 58 166 6.48e-31 113
MS.gene009272.t1 MTR_7g050710 30.526 285 149 10 22 259 28 310 1.88e-27 108
MS.gene009272.t1 MTR_3g061310 30.175 285 165 9 2 255 4 285 1.44e-26 105
MS.gene009272.t1 MTR_3g098790 29.749 279 149 11 23 259 29 302 2.51e-25 102
MS.gene009272.t1 MTR_0152s0100 42.982 114 62 1 147 260 2 112 2.89e-25 97.1
MS.gene009272.t1 MTR_0152s0100 42.982 114 62 1 147 260 2 112 2.89e-25 97.1
MS.gene009272.t1 MTR_3g061370 30.038 263 152 6 22 255 22 281 6.95e-25 100
MS.gene009272.t1 MTR_3g061320 32.210 267 144 10 19 255 20 279 1.05e-24 99.8
MS.gene009272.t1 MTR_3g061340 30.566 265 146 9 19 255 20 274 1.15e-24 99.8
MS.gene009272.t1 MTR_3g061350 30.147 272 148 9 19 255 20 284 3.28e-24 98.6
MS.gene009272.t1 MTR_1g034240 48.810 84 43 0 22 105 22 105 8.41e-24 99.8
MS.gene009272.t1 MTR_1g034240 37.879 132 69 4 133 257 268 393 1.31e-16 79.3
MS.gene009272.t1 MTR_1g108780 27.148 291 150 8 20 257 10 291 9.86e-24 99.8
MS.gene009272.t1 MTR_7g050560 26.007 273 158 5 23 259 29 293 2.30e-23 97.1
MS.gene009272.t1 MTR_8g103940 25.658 304 158 9 8 257 43 332 6.49e-23 97.8
MS.gene009272.t1 MTR_7g050590 24.828 290 157 7 23 259 2 283 1.20e-21 92.0
MS.gene009272.t1 MTR_7g050580 27.612 268 160 8 23 259 29 293 6.86e-21 90.1
MS.gene009272.t1 MTR_5g044670 24.101 278 167 6 23 259 32 306 9.32e-21 90.1
MS.gene009272.t1 MTR_1g035460 26.689 296 147 8 23 257 2 288 1.39e-20 90.5
MS.gene009272.t1 MTR_4g068320 38.835 103 62 1 4 105 2 104 5.79e-20 89.0
MS.gene009272.t1 MTR_4g068320 38.060 134 70 4 133 259 264 391 2.97e-17 80.9
MS.gene009272.t1 MTR_1g034210 49.333 75 38 0 22 96 22 96 7.73e-19 84.7
MS.gene009272.t1 MTR_1g034210 49.333 75 38 0 22 96 22 96 1.52e-18 84.3
MS.gene009272.t1 MTR_1g034210 35.484 124 67 4 141 257 242 359 1.83e-12 66.6
MS.gene009272.t1 MTR_3g061330 29.825 171 91 3 22 165 25 193 1.00e-17 80.5
MS.gene009272.t1 MTR_3g061290 41.509 106 59 2 19 122 20 124 1.03e-17 79.3
MS.gene009272.t1 MTR_7g050720 34.737 95 62 0 23 117 26 120 5.52e-15 73.9
MS.gene009272.t1 MTR_3g061220 37.736 106 50 3 2 105 4 95 2.38e-14 68.2
MS.gene009272.t1 MTR_7g050640 34.375 96 63 0 12 107 18 113 8.30e-14 66.6
MS.gene009272.t1 MTR_6g061070 23.288 292 161 8 23 255 34 321 1.76e-12 67.0
MS.gene009272.t1 MTR_4g119990 25.091 275 145 11 22 242 30 297 5.78e-12 65.5
MS.gene009272.t1 MTR_1g035510 30.612 98 65 2 23 120 2 96 4.05e-11 61.6
MS.gene009272.t1 MTR_1g035520 33.654 104 66 2 2 105 67 167 9.85e-11 61.6
MS.gene009272.t1 MTR_1g021290 38.202 89 54 1 12 100 33 120 9.85e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009272.t1 AT3G18990 44.898 98 51 1 8 105 10 104 2.54e-21 91.7
MS.gene009272.t1 AT3G18990 37.037 135 73 4 133 261 213 341 5.64e-16 77.0
MS.gene009272.t1 AT3G18990 44.898 98 51 1 8 105 10 104 5.11e-21 91.3
MS.gene009272.t1 AT3G18990 36.765 136 74 4 133 262 213 342 1.32e-15 75.9
MS.gene009272.t1 AT1G49475 40.625 96 56 1 20 115 47 141 1.75e-18 81.3
MS.gene009272.t1 AT3G18960 40.000 90 54 0 21 110 7 96 5.50e-18 79.3
MS.gene009272.t1 AT3G18960 38.542 96 56 1 12 107 38 130 7.31e-18 79.7
MS.gene009272.t1 AT4G01580 37.374 99 59 1 12 110 38 133 8.88e-18 79.3
MS.gene009272.t1 AT3G18960 38.542 96 56 1 12 107 38 130 1.05e-17 79.7
MS.gene009272.t1 AT1G49480 37.037 135 73 4 133 261 98 226 1.92e-16 76.6
MS.gene009272.t1 AT1G49480 37.037 135 73 4 133 261 98 226 1.92e-16 76.6
MS.gene009272.t1 AT1G49480 37.037 135 73 4 133 261 98 226 1.92e-16 76.6

Find 39 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTTCCCATTAGAATTT+TGG 0.142530 3.2:+37279 None:intergenic
ACTGCTCTCCTTGAGAATTT+TGG 0.150373 3.2:+37119 None:intergenic
AAGTGAGAGTCATGAGAAAA+AGG 0.253305 3.2:-37383 MS.gene009272:CDS
TTTGCAGAATACTATTCTAT+AGG 0.313465 3.2:-37571 MS.gene009272:CDS
CATTGCAGATGATGATGATT+TGG 0.324669 3.2:+37069 None:intergenic
TTGCAGAATACTATTCTATA+GGG 0.341018 3.2:-37570 MS.gene009272:CDS
TGGAGCTTTATAGGAACATT+TGG 0.361401 3.2:+37139 None:intergenic
TTCCTGTGTTTCAAATATGA+AGG 0.394296 3.2:-37538 MS.gene009272:CDS
TTCAAAACTCTAAGTACAAA+TGG 0.425055 3.2:-37457 MS.gene009272:CDS
GTCAGCCCTCCTCTAAAATT+CGG 0.439993 3.2:-37771 MS.gene009272:CDS
GGGATTTACTGTATTTCAAA+GGG 0.445823 3.2:-37035 MS.gene009272:CDS
CTGTTCCTGATGGTCGTGAT+TGG 0.459514 3.2:-37651 MS.gene009272:CDS
CAGTTCATCCGTTATAGTAT+AGG 0.459617 3.2:+37747 None:intergenic
ACTCTCACTTGTTTCAACCT+TGG 0.466400 3.2:+37396 None:intergenic
CATCTGCAATGATAATATCA+AGG 0.473557 3.2:-37057 MS.gene009272:CDS
GGGGATTTACTGTATTTCAA+AGG 0.481017 3.2:-37036 MS.gene009272:CDS
ATTTCCCAATCACGACCATC+AGG 0.484720 3.2:+37646 None:intergenic
AGTGTCATAGTCGTAGAAAA+AGG 0.486595 3.2:-37420 MS.gene009272:CDS
TGATGGTCGTGATTGGGAAA+TGG 0.490310 3.2:-37644 MS.gene009272:CDS
TGTTCCTGATGGTCGTGATT+GGG 0.496959 3.2:-37650 MS.gene009272:CDS
AATTCTAATGGGAAATTATG+TGG 0.499901 3.2:-37275 MS.gene009272:intron
CCCCTTCATATTTGAAACAC+AGG 0.508323 3.2:+37536 None:intergenic
AATTCGGTCCTATACTATAA+CGG 0.516718 3.2:-37755 MS.gene009272:CDS
GAATACTATTCTATAGGGTA+TGG 0.523564 3.2:-37565 MS.gene009272:CDS
TCCTGTGTTTCAAATATGAA+GGG 0.529278 3.2:-37537 MS.gene009272:CDS
AGGGGCAACAATCTGTCTCA+TGG 0.538258 3.2:-37016 MS.gene009272:CDS
AATGTGCAGTATGTTAATGC+TGG 0.551027 3.2:-37184 MS.gene009272:intron
ATCTGCAATGATAATATCAA+GGG 0.551519 3.2:-37056 MS.gene009272:CDS
ATAAAGCTCCAAAATTCTCA+AGG 0.564100 3.2:-37127 MS.gene009272:CDS
CCTGTGTTTCAAATATGAAG+GGG 0.591078 3.2:-37536 MS.gene009272:CDS
TGAGCTGATCAATAAGAAGC+CGG 0.592920 3.2:-36978 MS.gene009272:CDS
AAATTCTCAAGGAGAGCAGT+GGG 0.598086 3.2:-37116 MS.gene009272:CDS
AAAATTCTCAAGGAGAGCAG+TGG 0.599450 3.2:-37117 MS.gene009272:CDS
ACATGCTTCTGAAAGAGCAA+AGG 0.603971 3.2:-37344 MS.gene009272:CDS
TTCATATTTGAAACACAGGA+AGG 0.623348 3.2:+37540 None:intergenic
GCTACAATTACTGTTCCTGA+TGG 0.638622 3.2:-37661 MS.gene009272:CDS
GGATTTACTGTATTTCAAAG+GGG 0.674995 3.2:-37034 MS.gene009272:CDS
GAAATGGAACTGAAGAAATG+CGG 0.682734 3.2:-37628 MS.gene009272:CDS
TCTGCAATGATAATATCAAG+GGG 0.693916 3.2:-37055 MS.gene009272:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATGAGTTTATAAAAAGATT+TGG - chr3.2:37003-37022 MS.gene009272:CDS 20.0%
!!! AATCTTTTTATAAACTCATC+TGG + chr3.2:37003-37022 None:intergenic 20.0%
!!! ACATTTACTTTTGTAACAAA+TGG - chr3.2:37106-37125 MS.gene009272:CDS 20.0%
!!! AGAATTTTGGAACAAAAATA+AGG + chr3.2:37417-37436 None:intergenic 20.0%
!!! ATAATTTCCCATTAGAATTT+TGG + chr3.2:37430-37449 None:intergenic 20.0%
!!! GAATTTTGGAACAAAAATAA+GGG + chr3.2:37416-37435 None:intergenic 20.0%
!!! GTTTTTTGGATTAAATTCTT+TGG + chr3.2:37394-37413 None:intergenic 20.0%
!!! TTTTGTTCCAAAATTCTAAT+GGG - chr3.2:37420-37439 MS.gene009272:CDS 20.0%
!!! TTTTTGTTCCAAAATTCTAA+TGG - chr3.2:37419-37438 MS.gene009272:CDS 20.0%
! AATTCTAATGGGAAATTATG+TGG - chr3.2:37431-37450 MS.gene009272:CDS 25.0%
! ATCTGCAATGATAATATCAA+GGG - chr3.2:37650-37669 MS.gene009272:CDS 25.0%
! TTCAAAACTCTAAGTACAAA+TGG - chr3.2:37249-37268 MS.gene009272:intron 25.0%
! TTGCAGAATACTATTCTATA+GGG - chr3.2:37136-37155 MS.gene009272:CDS 25.0%
! TTTGCAGAATACTATTCTAT+AGG - chr3.2:37135-37154 MS.gene009272:CDS 25.0%
!!! GAACAAAAATAAGGGTTTTT+TGG + chr3.2:37408-37427 None:intergenic 25.0%
!!! TTTGTACTTAGAGTTTTGAA+TGG + chr3.2:37249-37268 None:intergenic 25.0%
AATTCGGTCCTATACTATAA+CGG - chr3.2:36951-36970 MS.gene009272:CDS 30.0%
ATAAAGCTCCAAAATTCTCA+AGG - chr3.2:37579-37598 MS.gene009272:CDS 30.0%
CATCTGCAATGATAATATCA+AGG - chr3.2:37649-37668 MS.gene009272:CDS 30.0%
GAATACTATTCTATAGGGTA+TGG - chr3.2:37141-37160 MS.gene009272:CDS 30.0%
GGATTTACTGTATTTCAAAG+GGG - chr3.2:37672-37691 MS.gene009272:CDS 30.0%
GGGATTTACTGTATTTCAAA+GGG - chr3.2:37671-37690 MS.gene009272:CDS 30.0%
TCCTGTGTTTCAAATATGAA+GGG - chr3.2:37169-37188 MS.gene009272:CDS 30.0%
TCTGCAATGATAATATCAAG+GGG - chr3.2:37651-37670 MS.gene009272:CDS 30.0%
TTCCTGTGTTTCAAATATGA+AGG - chr3.2:37168-37187 MS.gene009272:CDS 30.0%
! TTCATATTTGAAACACAGGA+AGG + chr3.2:37169-37188 None:intergenic 30.0%
!!! TGAGAATTTTGGAGCTTTAT+AGG + chr3.2:37579-37598 None:intergenic 30.0%
AAGTGAGAGTCATGAGAAAA+AGG - chr3.2:37323-37342 MS.gene009272:CDS 35.0%
AATGTGCAGTATGTTAATGC+TGG - chr3.2:37522-37541 MS.gene009272:CDS 35.0%
AGTGAGAGTCATGAGAAAAA+GGG - chr3.2:37324-37343 MS.gene009272:CDS 35.0%
AGTGTCATAGTCGTAGAAAA+AGG - chr3.2:37286-37305 MS.gene009272:CDS 35.0%
CAGTTCATCCGTTATAGTAT+AGG + chr3.2:36962-36981 None:intergenic 35.0%
CCTGTGTTTCAAATATGAAG+GGG - chr3.2:37170-37189 MS.gene009272:CDS 35.0%
GAAATGGAACTGAAGAAATG+CGG - chr3.2:37078-37097 MS.gene009272:CDS 35.0%
GGGGATTTACTGTATTTCAA+AGG - chr3.2:37670-37689 MS.gene009272:CDS 35.0%
TAATGGGAAATTATGTGGTG+AGG - chr3.2:37436-37455 MS.gene009272:CDS 35.0%
! AGTATAGGACCGAATTTTAG+AGG + chr3.2:36947-36966 None:intergenic 35.0%
! ATTGTGACTTTTAGCACATC+CGG + chr3.2:37750-37769 None:intergenic 35.0%
! CATTGCAGATGATGATGATT+TGG + chr3.2:37640-37659 None:intergenic 35.0%
! TGGAGCTTTATAGGAACATT+TGG + chr3.2:37570-37589 None:intergenic 35.0%
AAAATTCTCAAGGAGAGCAG+TGG - chr3.2:37589-37608 MS.gene009272:CDS 40.0%
AAATTCTCAAGGAGAGCAGT+GGG - chr3.2:37590-37609 MS.gene009272:CDS 40.0%
ACTCTCACTTGTTTCAACCT+TGG + chr3.2:37313-37332 None:intergenic 40.0%
GCTACAATTACTGTTCCTGA+TGG - chr3.2:37045-37064 MS.gene009272:CDS 40.0%
TAGTCGTAGAAAAAGGTCCA+AGG - chr3.2:37293-37312 MS.gene009272:CDS 40.0%
TGAGCTGATCAATAAGAAGC+CGG - chr3.2:37728-37747 MS.gene009272:CDS 40.0%
! ACATGCTTCTGAAAGAGCAA+AGG - chr3.2:37362-37381 MS.gene009272:CDS 40.0%
! ACTGCTCTCCTTGAGAATTT+TGG + chr3.2:37590-37609 None:intergenic 40.0%
! CCCCTTCATATTTGAAACAC+AGG + chr3.2:37173-37192 None:intergenic 40.0%
!! ATAGGACCGAATTTTAGAGG+AGG + chr3.2:36944-36963 None:intergenic 40.0%
!! TAGGACCGAATTTTAGAGGA+GGG + chr3.2:36943-36962 None:intergenic 40.0%
ATTTCCCAATCACGACCATC+AGG + chr3.2:37063-37082 None:intergenic 45.0%
TGATGGTCGTGATTGGGAAA+TGG - chr3.2:37062-37081 MS.gene009272:CDS 45.0%
TGTTCCTGATGGTCGTGATT+GGG - chr3.2:37056-37075 MS.gene009272:CDS 45.0%
!! GTCAGCCCTCCTCTAAAATT+CGG - chr3.2:36935-36954 MS.gene009272:CDS 45.0%
AGGGGCAACAATCTGTCTCA+TGG - chr3.2:37690-37709 MS.gene009272:CDS 50.0%
CTGTTCCTGATGGTCGTGAT+TGG - chr3.2:37055-37074 MS.gene009272:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 36931 37797 36931 ID=MS.gene009272
chr3.2 mRNA 36931 37797 36931 ID=MS.gene009272.t1;Parent=MS.gene009272
chr3.2 exon 37276 37797 37276 ID=MS.gene009272.t1.exon1;Parent=MS.gene009272.t1
chr3.2 CDS 37276 37797 37276 ID=cds.MS.gene009272.t1;Parent=MS.gene009272.t1
chr3.2 exon 36931 37197 36931 ID=MS.gene009272.t1.exon2;Parent=MS.gene009272.t1
chr3.2 CDS 36931 37197 36931 ID=cds.MS.gene009272.t1;Parent=MS.gene009272.t1
Gene Sequence

>MS.gene009272

ATGCGTCAGCCCTCCTCTAAAATTCGGTCCTATACTATAACGGATGAACTGTTATCACTGCAGAGAATTCCAGATGAGTTTATAAAAAGATTTGGAAATGAACTCAAGAATGTTGCTACAATTACTGTTCCTGATGGTCGTGATTGGGAAATGGAACTGAAGAAATGCGGCAACGACATTTACTTTTGTAACAAATGGCAACAGTTTGCAGAATACTATTCTATAGGGTATGGTTCCTTCCTGTGTTTCAAATATGAAGGGGATTCAAAGTTTAGTGTCGTTATATTTGATGTAACTTCTGTTGAGATTTGCTATCCATTCAAAACTCTAAGTACAAATGGAGAAACAAACACAAAGTGTCATAGTCGTAGAAAAAGGTCCAAGGTTGAAACAAGTGAGAGTCATGAGAAAAAGGGCAAAAGCATGTCTAAACATGCTTCTGAAAGAGCAAAGGAAGCTGCCAAAGAATTTAATCCAAAAAACCCTTATTTTTGTTCCAAAATTCTAATGGGAAATTATGTGGTGAGGATTTTACTTTAAAGCAAATGTTGAGTTTTTTATTTATGTTATCACTTTAATGATCTCTCATTTAATGTGCAGTATGTTAATGCTGGTTTTGCTTCAAAGCATCTGAATCCAAATGTTCCTATAAAGCTCCAAAATTCTCAAGGAGAGCAGTGGGAAGTTTCTTGTGCAATGCATGATGCCAAATCATCATCATCTGCAATGATAATATCAAGGGGATTTACTGTATTTCAAAGGGGCAACAATCTGTCTCATGGAGATTATTGTGTGTTTGAGCTGATCAATAAGAAGCCGGATGTGCTAAAAGTCACAATGTTTCGTGTGATTGACTATTGCGATTAA

Protein sequence

>MS.gene009272.t1

MRQPSSKIRSYTITDELLSLQRIPDEFIKRFGNELKNVATITVPDGRDWEMELKKCGNDIYFCNKWQQFAEYYSIGYGSFLCFKYEGDSKFSVVIFDVTSVEICYPFKTLSTNGETNTKCHSRRKRSKVETSESHEKKGKSMSKHASERAKEAAKEFNPKNPYFCSKILMGNYVYVNAGFASKHLNPNVPIKLQNSQGEQWEVSCAMHDAKSSSSAMIISRGFTVFQRGNNLSHGDYCVFELINKKPDVLKVTMFRVIDYCD