AlfalfaGEDB | MS.gene009315

M. sativa cultivar XinJiangDaYe / MS.gene009315

KEGG: K20791
GO: GO:0003674 GO:0003824 GO:0008080 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747
PFAM: PF00583.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009315.t1	GAU38899.1	39.9	183	40	5	4	116	2	184	1.60E-14	89

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009315.t1	Q9FKI4	35.7	154	45	4	4	103	2	155	9.2e-11	67.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009315.t1	A0A2Z6P5E3	39.9	183	40	5	4	116	2	184	1.2e-14	89.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene009315.t1	TR	GNAT

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009315.t1	MTR_3g034200	39.011	182	41	5	4	115	2	183	7.94e-25	93.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009315.t1	AT5G13780	35.714	154	45	4	4	103	2	155	2.13e-17	73.9

Find 31 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTGTTCCATGTCGTTT+TGG	0.275526	3.1:-1426995	None:intergenic
AAGGGGAAGCAGATGCATTC+TGG	0.323040	3.1:+1427766	MS.gene009315:CDS
CACGTCGTTGAAGATATTAA+TGG	0.376158	3.1:+1426849	MS.gene009315:CDS
TCTAAGATGTACAAACTCTT+TGG	0.380946	3.1:+1426786	MS.gene009315:CDS
AGGGGAAGCAGATGCATTCT+GGG	0.418741	3.1:+1427767	MS.gene009315:CDS
CTCCCAAAACGACATGGAAC+AGG	0.433249	3.1:+1426992	MS.gene009315:CDS
TGCATTCTGGGGAGACTAAA+GGG	0.439115	3.1:+1427779	MS.gene009315:CDS
TCTCTGATGGGTTGGGAGAA+CGG	0.508987	3.1:-1426940	None:intergenic
ATGCATTCTGGGGAGACTAA+AGG	0.509032	3.1:+1427778	MS.gene009315:CDS
AGCTTGTTGCCCTCTTCTCA+CGG	0.516174	3.1:+1426814	MS.gene009315:CDS
TACCACGTCTCTGATGGGTT+GGG	0.526888	3.1:-1426947	None:intergenic
TAGCGATACCACGTCTCTGA+TGG	0.539259	3.1:-1426953	None:intergenic
ATACCACGTCTCTGATGGGT+TGG	0.540598	3.1:-1426948	None:intergenic
GAGTTGAGGCCGTGAGAAGA+GGG	0.546949	3.1:-1426823	None:intergenic
ATGGGTTGGGAGAACGGCGA+GGG	0.570608	3.1:-1426934	None:intergenic
GAAATGAGAAAGCAACTCAA+GGG	0.572712	3.1:+1427748	MS.gene009315:CDS
TGAGTTGAGGCCGTGAGAAG+AGG	0.574852	3.1:-1426824	None:intergenic
TGAAATGAGAAAGCAACTCA+AGG	0.580702	3.1:+1427747	MS.gene009315:CDS
AGCGATACCACGTCTCTGAT+GGG	0.588160	3.1:-1426952	None:intergenic
GATTGCCTCCCAAAACGACA+TGG	0.596635	3.1:+1426986	MS.gene009315:CDS
ATTCAAGAGGAAATTAGTGA+AGG	0.600654	3.1:+1426900	MS.gene009315:CDS
GATGGGTTGGGAGAACGGCG+AGG	0.612867	3.1:-1426935	None:intergenic
CGTCCACTGTGAAATTCAAG+AGG	0.617845	3.1:+1426887	MS.gene009315:CDS
TTGAATTTCACAGTGGACGT+AGG	0.625067	3.1:-1426883	None:intergenic
AGAGTTTGTACATCTTAGAG+AGG	0.637261	3.1:-1426782	None:intergenic
CTCCCAACCCATCAGAGACG+TGG	0.645269	3.1:+1426945	MS.gene009315:CDS
AAATGAGAAAGCAACTCAAG+GGG	0.646235	3.1:+1427749	MS.gene009315:CDS
TCAACGACGTGAATGAGTTG+AGG	0.659923	3.1:-1426837	None:intergenic
GGGGAAGCAGATGCATTCTG+GGG	0.660086	3.1:+1427768	MS.gene009315:CDS
ACATCTTAGAGAGGTCGCAA+AGG	0.660670	3.1:-1426773	None:intergenic
TTTCCTCTTGAATTTCACAG+TGG	0.694893	3.1:-1426890	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATATATATGAAAAAAAATC+TGG	-	chr3.1:1427414-1427433	None:intergenic	10.0%
!!	ATATATATGAAAAAAAATCT+GGG	-	chr3.1:1427413-1427432	None:intergenic	10.0%
!!	TTCATATATATTATATAAGT+TGG	+	chr3.1:1427422-1427441	MS.gene009315:intron	10.0%
!!	TATATATGAAAAAAAATCTG+GGG	-	chr3.1:1427412-1427431	None:intergenic	15.0%
!	AATTAAACCATATAGTGCAA+TGG	-	chr3.1:1427624-1427643	None:intergenic	25.0%
!	TTGTATTTAACACAGATGTT+TGG	+	chr3.1:1427218-1427237	MS.gene009315:intron	25.0%
!!	AATTTGTTCAAAGAGACATT+GGG	+	chr3.1:1427290-1427309	MS.gene009315:intron	25.0%
!!!	TAATTTGTTCAAAGAGACAT+TGG	+	chr3.1:1427289-1427308	MS.gene009315:intron	25.0%
	AAAAAATCTGGGGAAAAATC+TGG	-	chr3.1:1427402-1427421	None:intergenic	30.0%
	ATTAATTCAAATTGCACCGA+GGG	+	chr3.1:1427068-1427087	MS.gene009315:intron	30.0%
	ATTCAAGAGGAAATTAGTGA+AGG	+	chr3.1:1426900-1426919	MS.gene009315:CDS	30.0%
	TATTAATTCAAATTGCACCG+AGG	+	chr3.1:1427067-1427086	MS.gene009315:intron	30.0%
	TGAACAAATTAAAAGCTGCA+TGG	-	chr3.1:1427280-1427299	None:intergenic	30.0%
	TTGTGTGTCTTCTTGTAATA+GGG	+	chr3.1:1427164-1427183	MS.gene009315:intron	30.0%
	TTTGTGTGTCTTCTTGTAAT+AGG	+	chr3.1:1427163-1427182	MS.gene009315:intron	30.0%
!	TCTAAGATGTACAAACTCTT+TGG	+	chr3.1:1426786-1426805	MS.gene009315:CDS	30.0%
!!	AAGTTTTATGCTGATGATCA+TGG	+	chr3.1:1427338-1427357	MS.gene009315:intron	30.0%
!!	AATATAAAAAAGGGTGTTGC+AGG	+	chr3.1:1427537-1427556	MS.gene009315:intron	30.0%
!!	AGTTTTATGCTGATGATCAT+GGG	+	chr3.1:1427339-1427358	MS.gene009315:intron	30.0%
!!	ATATAAAAAAGGGTGTTGCA+GGG	+	chr3.1:1427538-1427557	MS.gene009315:intron	30.0%
	AAATGAGAAAGCAACTCAAG+GGG	+	chr3.1:1427749-1427768	MS.gene009315:CDS	35.0%
	ACATTGGGATACAAGATTCA+TGG	+	chr3.1:1427305-1427324	MS.gene009315:intron	35.0%
	ACCATATAGTGCAATGGTAT+TGG	-	chr3.1:1427618-1427637	None:intergenic	35.0%
	AGAGTTTGTACATCTTAGAG+AGG	-	chr3.1:1426785-1426804	None:intergenic	35.0%
	CACGTCGTTGAAGATATTAA+TGG	+	chr3.1:1426849-1426868	MS.gene009315:CDS	35.0%
	GAAATGAGAAAGCAACTCAA+GGG	+	chr3.1:1427748-1427767	MS.gene009315:CDS	35.0%
	TCCAATACCATTGCACTATA+TGG	+	chr3.1:1427614-1427633	MS.gene009315:intron	35.0%
	TGAAATGAGAAAGCAACTCA+AGG	+	chr3.1:1427747-1427766	MS.gene009315:CDS	35.0%
	TTTCCTCTTGAATTTCACAG+TGG	-	chr3.1:1426893-1426912	None:intergenic	35.0%
!	ATATAGTGCAATGGTATTGG+AGG	-	chr3.1:1427615-1427634	None:intergenic	35.0%
!	TATTATATAAGTTGGCCGAG+TGG	+	chr3.1:1427430-1427449	MS.gene009315:intron	35.0%
!!	ATACAAGATTCATGGATTGG+AGG	+	chr3.1:1427313-1427332	MS.gene009315:intron	35.0%
!!!	TTGGAGGAAGCATTTTGTAT+TGG	-	chr3.1:1427599-1427618	None:intergenic	35.0%
	ATCTAGCATGAAACATGGTG+TGG	+	chr3.1:1427365-1427384	MS.gene009315:intron	40.0%
	ATCTGGGGAAAAATCTGGTA+TGG	-	chr3.1:1427397-1427416	None:intergenic	40.0%
	CGGATCTAGACATGTCATAT+TGG	-	chr3.1:1427699-1427718	None:intergenic	40.0%
	CTAGACATGTCATATTGGTG+TGG	-	chr3.1:1427694-1427713	None:intergenic	40.0%
	GAAACTGAGTTTGAATCCCT+CGG	-	chr3.1:1427087-1427106	None:intergenic	40.0%
	GGGATACAAGATTCATGGAT+TGG	+	chr3.1:1427310-1427329	MS.gene009315:intron	40.0%
	TTGAATTTCACAGTGGACGT+AGG	-	chr3.1:1426886-1426905	None:intergenic	40.0%
	TTTATGCTGATGATCATGGG+CGG	+	chr3.1:1427342-1427361	MS.gene009315:intron	40.0%
!	CTCAGTTTCTGAGAGTTACA+AGG	+	chr3.1:1427098-1427117	MS.gene009315:intron	40.0%
!!	AAACCTGTTCCATGTCGTTT+TGG	-	chr3.1:1426998-1427017	None:intergenic	40.0%
!!!	AACCTGTTCCATGTCGTTTT+GGG	-	chr3.1:1426997-1427016	None:intergenic	40.0%
!!!	GAGGAAGCATTTTGTATTGG+AGG	-	chr3.1:1427596-1427615	None:intergenic	40.0%
!!!	GCATTTTACCATGGTGTTAC+AGG	-	chr3.1:1427571-1427590	None:intergenic	40.0%
!!!	TTGGAGGAAGCATTTTACCA+TGG	-	chr3.1:1427580-1427599	None:intergenic	40.0%
	ACATCTTAGAGAGGTCGCAA+AGG	-	chr3.1:1426776-1426795	None:intergenic	45.0%
	CACACAACACACATACCACT+CGG	-	chr3.1:1427448-1427467	None:intergenic	45.0%
	CCAGTGTTCAACTCTTGTTG+AGG	+	chr3.1:1427482-1427501	MS.gene009315:intron	45.0%
	CCTCAACAAGAGTTGAACAC+TGG	-	chr3.1:1427485-1427504	None:intergenic	45.0%
	CGTCCACTGTGAAATTCAAG+AGG	+	chr3.1:1426887-1426906	MS.gene009315:CDS	45.0%
	GAATCGAACCTGTAACACCA+TGG	+	chr3.1:1427560-1427579	MS.gene009315:intron	45.0%
	TCAACGACGTGAATGAGTTG+AGG	-	chr3.1:1426840-1426859	None:intergenic	45.0%
!	ATGCATTCTGGGGAGACTAA+AGG	+	chr3.1:1427778-1427797	MS.gene009315:CDS	45.0%
!	TGCATTCTGGGGAGACTAAA+GGG	+	chr3.1:1427779-1427798	MS.gene009315:CDS	45.0%
!!	ATAAGTTATTAATATAAAAA+AGG	+	chr3.1:1427527-1427546	MS.gene009315:intron	5.0%
!!	TAAGTTATTAATATAAAAAA+GGG	+	chr3.1:1427528-1427547	MS.gene009315:intron	5.0%
	AAGGGGAAGCAGATGCATTC+TGG	+	chr3.1:1427766-1427785	MS.gene009315:CDS	50.0%
	AGCGATACCACGTCTCTGAT+GGG	-	chr3.1:1426955-1426974	None:intergenic	50.0%
	AGGGGAAGCAGATGCATTCT+GGG	+	chr3.1:1427767-1427786	MS.gene009315:CDS	50.0%
	CTCCCAAAACGACATGGAAC+AGG	+	chr3.1:1426992-1427011	MS.gene009315:CDS	50.0%
	GATTGCCTCCCAAAACGACA+TGG	+	chr3.1:1426986-1427005	MS.gene009315:CDS	50.0%
	GGCGGATCTAGCATGAAACA+TGG	+	chr3.1:1427360-1427379	MS.gene009315:intron	50.0%
	TAGCGATACCACGTCTCTGA+TGG	-	chr3.1:1426956-1426975	None:intergenic	50.0%
!	ATACCACGTCTCTGATGGGT+TGG	-	chr3.1:1426951-1426970	None:intergenic	50.0%
!!	AGCTTGTTGCCCTCTTCTCA+CGG	+	chr3.1:1426814-1426833	MS.gene009315:CDS	50.0%
!!	TACCACGTCTCTGATGGGTT+GGG	-	chr3.1:1426950-1426969	None:intergenic	50.0%
!!	TCTCTGATGGGTTGGGAGAA+CGG	-	chr3.1:1426943-1426962	None:intergenic	50.0%
!!!	CTGTTCCATGTCGTTTTGGG+AGG	-	chr3.1:1426994-1427013	None:intergenic	50.0%
	ATCCTCCCCATCATCAGCAG+GGG	-	chr3.1:1427722-1427741	None:intergenic	55.0%
	CATCCTCCCCATCATCAGCA+GGG	-	chr3.1:1427723-1427742	None:intergenic	55.0%
	GAGTTGAGGCCGTGAGAAGA+GGG	-	chr3.1:1426826-1426845	None:intergenic	55.0%
	GGGGAAGCAGATGCATTCTG+GGG	+	chr3.1:1427768-1427787	MS.gene009315:CDS	55.0%
!	TGAGTTGAGGCCGTGAGAAG+AGG	-	chr3.1:1426827-1426846	None:intergenic	55.0%
	AGATCCGCCCCTGCTGATGA+TGG	+	chr3.1:1427712-1427731	MS.gene009315:intron	60.0%
	CTCCCAACCCATCAGAGACG+TGG	+	chr3.1:1426945-1426964	MS.gene009315:CDS	60.0%
	GATCCGCCCCTGCTGATGAT+GGG	+	chr3.1:1427713-1427732	MS.gene009315:intron	60.0%
	GCATCCTCCCCATCATCAGC+AGG	-	chr3.1:1427724-1427743	None:intergenic	60.0%
!	ATCCGCCCCTGCTGATGATG+GGG	+	chr3.1:1427714-1427733	MS.gene009315:intron	60.0%
!!	ATGGGTTGGGAGAACGGCGA+GGG	-	chr3.1:1426937-1426956	None:intergenic	60.0%
	CTCCCCATCATCAGCAGGGG+CGG	-	chr3.1:1427719-1427738	None:intergenic	65.0%
!!	GATGGGTTGGGAGAACGGCG+AGG	-	chr3.1:1426938-1426957	None:intergenic	65.0%
!!	CGCCCCTGCTGATGATGGGG+AGG	+	chr3.1:1427717-1427736	MS.gene009315:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	1426744	1427819	1426744	ID=MS.gene009315
chr3.1	mRNA	1426744	1427819	1426744	ID=MS.gene009315.t1;Parent=MS.gene009315
chr3.1	exon	1426744	1427013	1426744	ID=MS.gene009315.t1.exon1;Parent=MS.gene009315.t1
chr3.1	CDS	1426744	1427013	1426744	ID=cds.MS.gene009315.t1;Parent=MS.gene009315.t1
chr3.1	exon	1427739	1427819	1427739	ID=MS.gene009315.t1.exon2;Parent=MS.gene009315.t1
chr3.1	CDS	1427739	1427819	1427739	ID=cds.MS.gene009315.t1;Parent=MS.gene009315.t1

Gene Sequence

>MS.gene009315

ATGTCTCAAGTGTGCATAAGAGAAGCAAACCTTTGCGACCTCTCTAAGATGTACAAACTCTTTGGTCTAAAGCTTGTTGCCCTCTTCTCACGGCCTCAACTCATTCACGTCGTTGAAGATATTAATGGTCACATCGTCGCCTACGTCCACTGTGAAATTCAAGAGGAAATTAGTGAAGGAGCTATTCATTCCCTCGCCGTTCTCCCAACCCATCAGAGACGTGGTATCGCTACTAAGCTTGTGATTGCCTCCCAAAACGACATGGAACAGGTTTGTTTGTTTGAGTGTTCAATTACAAGTCTGCTATTGATATTGTGAGTTTTTATTAATTCAAATTGCACCGAGGGATTCAAACTCAGTTTCTGAGAGTTACAAGGCTTAAATGTCTTATTGATACTAACGATCGTGTCTGTTCGTTCTTTGTGTGTCTTCTTGTAATAGGGATATGTGTAAACTTTAATATTGTTACTGTGTTTGTATTTAACACAGATGTTTGGTTCTGAGTACGTCTCAGTGCATGTCAGAAAGAGCAACCATGCAGCTTTTAATTTGTTCAAAGAGACATTGGGATACAAGATTCATGGATTGGAGGCAAAGTTTTATGCTGATGATCATGGGCGGATCTAGCATGAAACATGGTGTGGCAGTTGCCATACCAGATTTTTCCCCAGATTTTTTTTCATATATATTATATAAGTTGGCCGAGTGGTATGTGTGTTGTGTGAATGATATGCATGTCCAGTGTTCAACTCTTGTTGAGGTGTGCTTTTGAACAAATTTTTTATAAGTTATTAATATAAAAAAGGGTGTTGCAGGGAATCGAACCTGTAACACCATGGTAAAATGCTTCCTCCAATACAAAATGCTTCCTCCAATACCATTGCACTATATGGTTTAATTCATCAATAACTTACTTTGTTAATAAATATAGTTGATTTGTATATTTGCCACACCAATATGACATGTCTAGATCCGCCCCTGCTGATGATGGGGAGGATGCTTATGAAATGAGAAAGCAACTCAAGGGGAAGCAGATGCATTCTGGGGAGACTAAAGGGAATGCAAAAGCCACATGA

Protein sequence

>MS.gene009315.t1

MSQVCIREANLCDLSKMYKLFGLKLVALFSRPQLIHVVEDINGHIVAYVHCEIQEEISEGAIHSLAVLPTHQRRGIATKLVIASQNDMEQDAYEMRKQLKGKQMHSGETKGNAKAT