Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009415.t1 | XP_003598191.1 | 87.6 | 275 | 34 | 0 | 1 | 275 | 1 | 275 | 4.90E-94 | 354.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009415.t1 | G7IVM3 | 87.6 | 275 | 34 | 0 | 1 | 275 | 1 | 275 | 3.5e-94 | 354.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene009415.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009415.t1 | MTR_3g008310 | 85.091 | 275 | 41 | 0 | 1 | 275 | 1 | 275 | 1.33e-174 | 483 |
| MS.gene009415.t1 | MTR_3g408380 | 80.071 | 281 | 38 | 3 | 1 | 275 | 1 | 269 | 1.02e-161 | 450 |
| MS.gene009415.t1 | MTR_3g408420 | 64.875 | 279 | 90 | 3 | 1 | 275 | 1 | 275 | 2.87e-116 | 335 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009415.t1 | AT3G60580 | 24.373 | 279 | 153 | 9 | 8 | 266 | 6 | 246 | 8.02e-12 | 64.7 |
Find 41 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATACTTGGTTGGTGATT+GGG | 0.262501 | 3.4:-3562292 | None:intergenic |
| AAAATTAGTGTATAGTGATT+TGG | 0.310655 | 3.4:+3562634 | MS.gene009415:CDS |
| TTGTGTATAGAGTTATTTCT+TGG | 0.316350 | 3.4:-3562383 | None:intergenic |
| TGCTTTGAAATCTTTGAATC+TGG | 0.324623 | 3.4:+3562923 | MS.gene009415:CDS |
| CTTTGAATCTGGTGAGGTAT+TGG | 0.325399 | 3.4:+3562934 | MS.gene009415:CDS |
| TTTCTTGGATTAACAATTGA+TGG | 0.326711 | 3.4:-3562368 | None:intergenic |
| AACAATTTAGCAAGAGAGTT+TGG | 0.341571 | 3.4:+3562845 | MS.gene009415:CDS |
| TCAGATACTTGGTTGGTGAT+TGG | 0.358861 | 3.4:-3562293 | None:intergenic |
| GCAAGAGAGTTTGGTAGAAG+TGG | 0.402120 | 3.4:+3562854 | MS.gene009415:CDS |
| CAACCGAGTATTCAGATACT+TGG | 0.408432 | 3.4:-3562304 | None:intergenic |
| TGTCATGAAGTGTTTCAATC+TGG | 0.412291 | 3.4:+3562782 | MS.gene009415:CDS |
| GATAGAGTGAGTTGGATGTT+TGG | 0.448812 | 3.4:-3562333 | None:intergenic |
| TATTCCTACCAAAGTTTGAC+AGG | 0.460181 | 3.4:-3562439 | None:intergenic |
| CACAATCTTCAATCTTTGAA+AGG | 0.463545 | 3.4:+3562401 | MS.gene009415:CDS |
| AGATACTTGGTTGGTGATTG+GGG | 0.466978 | 3.4:-3562291 | None:intergenic |
| AACACGGAATTTAATGTGGC+TGG | 0.469241 | 3.4:+3562587 | MS.gene009415:CDS |
| AATTGTGCCCTGTCAAACTT+TGG | 0.474964 | 3.4:+3562431 | MS.gene009415:CDS |
| ACAATCTTCAATCTTTGAAA+GGG | 0.478955 | 3.4:+3562402 | MS.gene009415:CDS |
| TCTTATCCAACAAACCCAAC+TGG | 0.484776 | 3.4:+3562473 | MS.gene009415:CDS |
| ATATAATGTTGTAAATGAAA+AGG | 0.485017 | 3.4:+3562883 | MS.gene009415:CDS |
| GGATTCGTAATTTGGCATAG+TGG | 0.490151 | 3.4:-3562259 | None:intergenic |
| CAAACCAAGACAATTTCATA+TGG | 0.492782 | 3.4:+3562511 | MS.gene009415:CDS |
| GATGTAGAGATAGAGTGAGT+TGG | 0.497788 | 3.4:-3562341 | None:intergenic |
| TTAGCCATATGAAATTGTCT+TGG | 0.500224 | 3.4:-3562515 | None:intergenic |
| GTGTTATGCGTAAGAAAGAA+TGG | 0.501519 | 3.4:+3562681 | MS.gene009415:CDS |
| TGAATCTGGTGAGGTATTGG+AGG | 0.506890 | 3.4:+3562937 | MS.gene009415:CDS |
| CAACCAAGTATCTGAATACT+CGG | 0.520019 | 3.4:+3562301 | MS.gene009415:CDS |
| GATTCGTAATTTGGCATAGT+GGG | 0.532810 | 3.4:-3562258 | None:intergenic |
| TCCTCCCAAAGCTTTCCCAT+TGG | 0.533471 | 3.4:-3562225 | None:intergenic |
| ATCTGAATACTCGGTTGAGT+TGG | 0.542063 | 3.4:+3562310 | MS.gene009415:CDS |
| CGAGTATTCAGATACTTGGT+TGG | 0.556849 | 3.4:-3562300 | None:intergenic |
| GAAAGAATGGAAACAAATTG+AGG | 0.561440 | 3.4:+3562694 | MS.gene009415:CDS |
| GTGCCCTGTCAAACTTTGGT+AGG | 0.575115 | 3.4:+3562435 | MS.gene009415:CDS |
| GAGTGTGTCTCATTTGTCAC+AGG | 0.578392 | 3.4:+3562195 | MS.gene009415:CDS |
| TGAAATCTTTGAATCTGGTG+AGG | 0.581368 | 3.4:+3562928 | MS.gene009415:CDS |
| GGTATTGGAGGAACACATGA+AGG | 0.590503 | 3.4:+3562949 | MS.gene009415:CDS |
| TCCAATGGGAAAGCTTTGGG+AGG | 0.618334 | 3.4:+3562224 | MS.gene009415:CDS |
| TCTCCAACTATGGAGAACAA+TGG | 0.649885 | 3.4:+3562170 | None:intergenic |
| AGAGAACACGGAATTTAATG+TGG | 0.656156 | 3.4:+3562583 | MS.gene009415:CDS |
| TGAAGAAAGAGAAGAGAACA+CGG | 0.658044 | 3.4:+3562571 | MS.gene009415:CDS |
| ATTCCTACCAAAGTTTGACA+GGG | 0.694573 | 3.4:-3562438 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAATGTTGTAAATGAAA+AGG | + | chr3.4:3562883-3562902 | MS.gene009415:CDS | 15.0% |
| !!! | CTAAATTGTTAGTTTTATTA+TGG | - | chr3.4:3562835-3562854 | None:intergenic | 15.0% |
| !! | AAAATTAGTGTATAGTGATT+TGG | + | chr3.4:3562634-3562653 | MS.gene009415:CDS | 20.0% |
| ! | AAGAAAAAAGCAAGTGAAAA+TGG | + | chr3.4:3562734-3562753 | MS.gene009415:CDS | 25.0% |
| ! | TTTCTTGGATTAACAATTGA+TGG | - | chr3.4:3562371-3562390 | None:intergenic | 25.0% |
| !! | ACAATCTTCAATCTTTGAAA+GGG | + | chr3.4:3562402-3562421 | MS.gene009415:CDS | 25.0% |
| !!! | TTGTGTATAGAGTTATTTCT+TGG | - | chr3.4:3562386-3562405 | None:intergenic | 25.0% |
| AACAATTTAGCAAGAGAGTT+TGG | + | chr3.4:3562845-3562864 | MS.gene009415:CDS | 30.0% | |
| CAAACCAAGACAATTTCATA+TGG | + | chr3.4:3562511-3562530 | MS.gene009415:CDS | 30.0% | |
| CACAATCTTCAATCTTTGAA+AGG | + | chr3.4:3562401-3562420 | MS.gene009415:CDS | 30.0% | |
| GAAAGAATGGAAACAAATTG+AGG | + | chr3.4:3562694-3562713 | MS.gene009415:CDS | 30.0% | |
| TTAGCCATATGAAATTGTCT+TGG | - | chr3.4:3562518-3562537 | None:intergenic | 30.0% | |
| ! | CAATCTGGTAAAGATCTTTT+CGG | + | chr3.4:3562797-3562816 | MS.gene009415:CDS | 30.0% |
| !! | GTTTTGATCTTTTTCCAGTT+GGG | - | chr3.4:3562490-3562509 | None:intergenic | 30.0% |
| !! | TGCTTTGAAATCTTTGAATC+TGG | + | chr3.4:3562923-3562942 | MS.gene009415:CDS | 30.0% |
| AGAGAACACGGAATTTAATG+TGG | + | chr3.4:3562583-3562602 | MS.gene009415:CDS | 35.0% | |
| ATTCCTACCAAAGTTTGACA+GGG | - | chr3.4:3562441-3562460 | None:intergenic | 35.0% | |
| CAACCAAGTATCTGAATACT+CGG | + | chr3.4:3562301-3562320 | MS.gene009415:CDS | 35.0% | |
| GATTCGTAATTTGGCATAGT+GGG | - | chr3.4:3562261-3562280 | None:intergenic | 35.0% | |
| GTGTTATGCGTAAGAAAGAA+TGG | + | chr3.4:3562681-3562700 | MS.gene009415:CDS | 35.0% | |
| TATTCCTACCAAAGTTTGAC+AGG | - | chr3.4:3562442-3562461 | None:intergenic | 35.0% | |
| TGAAATCTTTGAATCTGGTG+AGG | + | chr3.4:3562928-3562947 | MS.gene009415:CDS | 35.0% | |
| TGAAGAAAGAGAAGAGAACA+CGG | + | chr3.4:3562571-3562590 | MS.gene009415:CDS | 35.0% | |
| ! | ATTTTCCAATGGGAAAGCTT+TGG | + | chr3.4:3562220-3562239 | MS.gene009415:CDS | 35.0% |
| ! | TGTCATGAAGTGTTTCAATC+TGG | + | chr3.4:3562782-3562801 | MS.gene009415:CDS | 35.0% |
| ! | TTTTCCAATGGGAAAGCTTT+GGG | + | chr3.4:3562221-3562240 | MS.gene009415:CDS | 35.0% |
| !! | CGTTTTGATCTTTTTCCAGT+TGG | - | chr3.4:3562491-3562510 | None:intergenic | 35.0% |
| !! | TTTTGGAGGGATTCGTAATT+TGG | - | chr3.4:3562270-3562289 | None:intergenic | 35.0% |
| AACACGGAATTTAATGTGGC+TGG | + | chr3.4:3562587-3562606 | MS.gene009415:CDS | 40.0% | |
| AATTGTGCCCTGTCAAACTT+TGG | + | chr3.4:3562431-3562450 | MS.gene009415:CDS | 40.0% | |
| ATCTGAATACTCGGTTGAGT+TGG | + | chr3.4:3562310-3562329 | MS.gene009415:CDS | 40.0% | |
| CAACCGAGTATTCAGATACT+TGG | - | chr3.4:3562307-3562326 | None:intergenic | 40.0% | |
| CGAGTATTCAGATACTTGGT+TGG | - | chr3.4:3562303-3562322 | None:intergenic | 40.0% | |
| CTTTGAATCTGGTGAGGTAT+TGG | + | chr3.4:3562934-3562953 | MS.gene009415:CDS | 40.0% | |
| GATGTAGAGATAGAGTGAGT+TGG | - | chr3.4:3562344-3562363 | None:intergenic | 40.0% | |
| GGATTCGTAATTTGGCATAG+TGG | - | chr3.4:3562262-3562281 | None:intergenic | 40.0% | |
| TCTTATCCAACAAACCCAAC+TGG | + | chr3.4:3562473-3562492 | MS.gene009415:CDS | 40.0% | |
| ! | CTTTTTCCAGTTGGGTTTGT+TGG | - | chr3.4:3562482-3562501 | None:intergenic | 40.0% |
| ! | GATAGAGTGAGTTGGATGTT+TGG | - | chr3.4:3562336-3562355 | None:intergenic | 40.0% |
| !! | AGATACTTGGTTGGTGATTG+GGG | - | chr3.4:3562294-3562313 | None:intergenic | 40.0% |
| !! | CAGATACTTGGTTGGTGATT+GGG | - | chr3.4:3562295-3562314 | None:intergenic | 40.0% |
| !! | GTCACAGGTCATTTTCCAAT+GGG | + | chr3.4:3562210-3562229 | MS.gene009415:CDS | 40.0% |
| !! | TCAGATACTTGGTTGGTGAT+TGG | - | chr3.4:3562296-3562315 | None:intergenic | 40.0% |
| !! | TGTCACAGGTCATTTTCCAA+TGG | + | chr3.4:3562209-3562228 | MS.gene009415:CDS | 40.0% |
| GAGTGTGTCTCATTTGTCAC+AGG | + | chr3.4:3562195-3562214 | MS.gene009415:CDS | 45.0% | |
| GCAAGAGAGTTTGGTAGAAG+TGG | + | chr3.4:3562854-3562873 | MS.gene009415:CDS | 45.0% | |
| ! | GGTATTGGAGGAACACATGA+AGG | + | chr3.4:3562949-3562968 | MS.gene009415:CDS | 45.0% |
| !! | TGAATCTGGTGAGGTATTGG+AGG | + | chr3.4:3562937-3562956 | MS.gene009415:CDS | 45.0% |
| !! | TGGTGATTGGGGTTTTTGGA+GGG | - | chr3.4:3562283-3562302 | None:intergenic | 45.0% |
| !! | TTGGTGATTGGGGTTTTTGG+AGG | - | chr3.4:3562284-3562303 | None:intergenic | 45.0% |
| !!! | TGGTTGGTGATTGGGGTTTT+TGG | - | chr3.4:3562287-3562306 | None:intergenic | 45.0% |
| GTGCCCTGTCAAACTTTGGT+AGG | + | chr3.4:3562435-3562454 | MS.gene009415:CDS | 50.0% | |
| TCCTCCCAAAGCTTTCCCAT+TGG | - | chr3.4:3562228-3562247 | None:intergenic | 50.0% | |
| !! | TCCAATGGGAAAGCTTTGGG+AGG | + | chr3.4:3562224-3562243 | MS.gene009415:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 3562179 | 3563006 | 3562179 | ID=MS.gene009415 |
| chr3.4 | mRNA | 3562179 | 3563006 | 3562179 | ID=MS.gene009415.t1;Parent=MS.gene009415 |
| chr3.4 | exon | 3562179 | 3563006 | 3562179 | ID=MS.gene009415.t1.exon1;Parent=MS.gene009415.t1 |
| chr3.4 | CDS | 3562179 | 3563006 | 3562179 | ID=cds.MS.gene009415.t1;Parent=MS.gene009415.t1 |
| Gene Sequence |
| Protein sequence |
