Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009420.t1 | XP_013459206.1 | 79.6 | 269 | 49 | 1 | 1 | 263 | 1 | 269 | 6.30E-99 | 370.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009420.t1 | Q9M202 | 26.0 | 269 | 149 | 10 | 8 | 254 | 6 | 246 | 1.4e-09 | 65.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009420.t1 | A0A072V3Z9 | 79.6 | 269 | 49 | 1 | 1 | 263 | 1 | 269 | 4.5e-99 | 370.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene009420.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009420.t1 | MTR_3g408380 | 91.078 | 269 | 18 | 1 | 1 | 263 | 1 | 269 | 0.0 | 505 |
| MS.gene009420.t1 | MTR_3g008310 | 84.000 | 275 | 32 | 1 | 1 | 263 | 1 | 275 | 1.02e-169 | 469 |
| MS.gene009420.t1 | MTR_3g408420 | 64.493 | 276 | 84 | 3 | 1 | 263 | 1 | 275 | 7.92e-114 | 328 |
| MS.gene009420.t1 | MTR_0160s0030 | 26.797 | 306 | 164 | 10 | 8 | 255 | 6 | 309 | 8.79e-14 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009420.t1 | AT3G60580 | 26.766 | 269 | 147 | 10 | 8 | 254 | 6 | 246 | 1.59e-13 | 69.3 |
| MS.gene009420.t1 | AT1G02030 | 26.482 | 253 | 152 | 8 | 8 | 254 | 7 | 231 | 1.58e-12 | 66.2 |
Find 43 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTGGTGGGTTACTTCT+TGG | 0.249003 | 3.4:-3643570 | None:intergenic |
| CAGGTACTTGGTTGTTGATT+GGG | 0.275325 | 3.4:-3643479 | None:intergenic |
| TCAGGTACTTGGTTGTTGAT+TGG | 0.290946 | 3.4:-3643480 | None:intergenic |
| CTTCTTGGATTAACAATTGA+TGG | 0.303146 | 3.4:-3643555 | None:intergenic |
| GCCAACTCAGCTGAGTATTC+AGG | 0.304238 | 3.4:-3643498 | None:intergenic |
| TGCTTTGACATCTTTGAATC+TGG | 0.309104 | 3.4:+3644074 | MS.gene009420:CDS |
| TTCAAAGATTGAGGATTGTT+TGG | 0.332480 | 3.4:-3643585 | None:intergenic |
| CTTTGAATCTGGTCACGTAT+TGG | 0.390852 | 3.4:+3644085 | MS.gene009420:CDS |
| ACAATCAGCGTATAGCGATT+TGG | 0.398813 | 3.4:+3643785 | MS.gene009420:CDS |
| CAGCTGAGTATTCAGGTACT+TGG | 0.400249 | 3.4:-3643491 | None:intergenic |
| GAGTGTGTCTCATTTGTAAC+AGG | 0.418825 | 3.4:+3643382 | MS.gene009420:CDS |
| AGGTACTTGGTTGTTGATTG+GGG | 0.434003 | 3.4:-3643478 | None:intergenic |
| CAGAATCATAATAATTGTAT+AGG | 0.437191 | 3.4:-3644127 | None:intergenic |
| GTGTTATTCTTAAGAATGAA+TGG | 0.439493 | 3.4:+3643832 | MS.gene009420:CDS |
| AATTTCACTTTCAAAGATTG+AGG | 0.444853 | 3.4:-3643594 | None:intergenic |
| AAATGTACCCCGTCAAACTT+TGG | 0.446972 | 3.4:+3643618 | MS.gene009420:CDS |
| GATAGAGTGAGTTGGATGTT+TGG | 0.448812 | 3.4:-3643520 | None:intergenic |
| AACAATCTAGCAGGAGAGAT+TGG | 0.456742 | 3.4:+3643996 | MS.gene009420:CDS |
| GGATTGGTAGTTTGGCATAG+TGG | 0.460724 | 3.4:-3643446 | None:intergenic |
| TCTTATCCAACAAACCCAAC+TGG | 0.466226 | 3.4:+3643660 | MS.gene009420:CDS |
| TATTCCTACCAAAGTTTGAC+GGG | 0.471579 | 3.4:-3643626 | None:intergenic |
| AAGATTGAGGATTGTTTGGT+GGG | 0.486813 | 3.4:-3643581 | None:intergenic |
| TCAGCCATATGAAACTGTCT+TGG | 0.489788 | 3.4:-3643702 | None:intergenic |
| CTATTCCTACCAAAGTTTGA+CGG | 0.495952 | 3.4:-3643627 | None:intergenic |
| GATGTAGAGATAGAGTGAGT+TGG | 0.497788 | 3.4:-3643528 | None:intergenic |
| TGAATCTGGTCACGTATTGG+AGG | 0.509112 | 3.4:+3644088 | MS.gene009420:CDS |
| AAAGATTGAGGATTGTTTGG+TGG | 0.514150 | 3.4:-3643582 | None:intergenic |
| GATTGGTAGTTTGGCATAGT+GGG | 0.520357 | 3.4:-3643445 | None:intergenic |
| CAAACCAAGACAGTTTCATA+TGG | 0.521057 | 3.4:+3643698 | MS.gene009420:CDS |
| GCAGGAGAGATTGGTAGAAG+TGG | 0.524466 | 3.4:+3644005 | MS.gene009420:CDS |
| AGGAAAACTAACAATCTAGC+AGG | 0.531732 | 3.4:+3643987 | MS.gene009420:CDS |
| ACCTGAATACTCAGCTGAGT+TGG | 0.539000 | 3.4:+3643497 | MS.gene009420:CDS |
| CGTATTGGAGGAACACATGA+AGG | 0.564625 | 3.4:+3644100 | MS.gene009420:CDS |
| GGTACTTGGTTGTTGATTGG+GGG | 0.573211 | 3.4:-3643477 | None:intergenic |
| GAATGAATGGAAACAAATTG+AGG | 0.585752 | 3.4:+3643845 | MS.gene009420:CDS |
| TCCAATGGAAAAGCTTTGGG+AGG | 0.590411 | 3.4:+3643411 | MS.gene009420:CDS |
| TCGGACATGAAGATATCCAT+AGG | 0.592226 | 3.4:+3643967 | MS.gene009420:CDS |
| ATAGAAGCTGCTGAAACACT+AGG | 0.609115 | 3.4:+3643810 | MS.gene009420:CDS |
| GTACCCCGTCAAACTTTGGT+AGG | 0.631191 | 3.4:+3643622 | MS.gene009420:CDS |
| ACAAATATCACACTCAAACA+CGG | 0.640237 | 3.4:-3643913 | None:intergenic |
| TCTCCAACTATGGAGAACAA+TGG | 0.649885 | 3.4:+3643357 | None:intergenic |
| AGAGAATATAGAATTTAACA+TGG | 0.705093 | 3.4:+3643734 | MS.gene009420:CDS |
| ATTCCTACCAAAGTTTGACG+GGG | 0.741422 | 3.4:-3643625 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAGAATATAGAATTTAACA+TGG | + | chr3.4:3643734-3643753 | MS.gene009420:CDS | 20.0% |
| !! | CAGAATCATAATAATTGTAT+AGG | - | chr3.4:3644130-3644149 | None:intergenic | 20.0% |
| ! | AAGAAAAAAGCAAGTGAAAA+TGG | + | chr3.4:3643885-3643904 | MS.gene009420:CDS | 25.0% |
| ! | AATTTCACTTTCAAAGATTG+AGG | - | chr3.4:3643597-3643616 | None:intergenic | 25.0% |
| !! | GTGTTATTCTTAAGAATGAA+TGG | + | chr3.4:3643832-3643851 | MS.gene009420:CDS | 25.0% |
| ACAAATATCACACTCAAACA+CGG | - | chr3.4:3643916-3643935 | None:intergenic | 30.0% | |
| CTTCTTGGATTAACAATTGA+TGG | - | chr3.4:3643558-3643577 | None:intergenic | 30.0% | |
| GAATGAATGGAAACAAATTG+AGG | + | chr3.4:3643845-3643864 | MS.gene009420:CDS | 30.0% | |
| TTCAAAGATTGAGGATTGTT+TGG | - | chr3.4:3643588-3643607 | None:intergenic | 30.0% | |
| ! | ATTTTCCAATGGAAAAGCTT+TGG | + | chr3.4:3643407-3643426 | MS.gene009420:CDS | 30.0% |
| ! | CAATCTAGCAAAGATCTTTT+CGG | + | chr3.4:3643948-3643967 | MS.gene009420:CDS | 30.0% |
| ! | TTTTCCAATGGAAAAGCTTT+GGG | + | chr3.4:3643408-3643427 | MS.gene009420:CDS | 30.0% |
| !! | GTTTTGATCTTTTTCCAGTT+GGG | - | chr3.4:3643677-3643696 | None:intergenic | 30.0% |
| !!! | CTAGATTGTTAGTTTTCCTA+TGG | - | chr3.4:3643986-3644005 | None:intergenic | 30.0% |
| AAAGATTGAGGATTGTTTGG+TGG | - | chr3.4:3643585-3643604 | None:intergenic | 35.0% | |
| AAGATTGAGGATTGTTTGGT+GGG | - | chr3.4:3643584-3643603 | None:intergenic | 35.0% | |
| AGGAAAACTAACAATCTAGC+AGG | + | chr3.4:3643987-3644006 | MS.gene009420:CDS | 35.0% | |
| CAAACCAAGACAGTTTCATA+TGG | + | chr3.4:3643698-3643717 | MS.gene009420:CDS | 35.0% | |
| CTATTCCTACCAAAGTTTGA+CGG | - | chr3.4:3643630-3643649 | None:intergenic | 35.0% | |
| TATTCCTACCAAAGTTTGAC+GGG | - | chr3.4:3643629-3643648 | None:intergenic | 35.0% | |
| TGCTTTGACATCTTTGAATC+TGG | + | chr3.4:3644074-3644093 | MS.gene009420:CDS | 35.0% | |
| !! | CGTTTTGATCTTTTTCCAGT+TGG | - | chr3.4:3643678-3643697 | None:intergenic | 35.0% |
| !! | TGTAACAGGTCATTTTCCAA+TGG | + | chr3.4:3643396-3643415 | MS.gene009420:CDS | 35.0% |
| !!! | TTTTGGATGGATTGGTAGTT+TGG | - | chr3.4:3643457-3643476 | None:intergenic | 35.0% |
| AAATGTACCCCGTCAAACTT+TGG | + | chr3.4:3643618-3643637 | MS.gene009420:CDS | 40.0% | |
| AACAATCTAGCAGGAGAGAT+TGG | + | chr3.4:3643996-3644015 | MS.gene009420:CDS | 40.0% | |
| ACAATCAGCGTATAGCGATT+TGG | + | chr3.4:3643785-3643804 | MS.gene009420:CDS | 40.0% | |
| ATAGAAGCTGCTGAAACACT+AGG | + | chr3.4:3643810-3643829 | MS.gene009420:CDS | 40.0% | |
| ATTCCTACCAAAGTTTGACG+GGG | - | chr3.4:3643628-3643647 | None:intergenic | 40.0% | |
| CTTTGAATCTGGTCACGTAT+TGG | + | chr3.4:3644085-3644104 | MS.gene009420:CDS | 40.0% | |
| GAGTGTGTCTCATTTGTAAC+AGG | + | chr3.4:3643382-3643401 | MS.gene009420:CDS | 40.0% | |
| GATGTAGAGATAGAGTGAGT+TGG | - | chr3.4:3643531-3643550 | None:intergenic | 40.0% | |
| TCAGCCATATGAAACTGTCT+TGG | - | chr3.4:3643705-3643724 | None:intergenic | 40.0% | |
| TCAGGTACTTGGTTGTTGAT+TGG | - | chr3.4:3643483-3643502 | None:intergenic | 40.0% | |
| TCGGACATGAAGATATCCAT+AGG | + | chr3.4:3643967-3643986 | MS.gene009420:CDS | 40.0% | |
| TCTTATCCAACAAACCCAAC+TGG | + | chr3.4:3643660-3643679 | MS.gene009420:CDS | 40.0% | |
| ! | AGGTACTTGGTTGTTGATTG+GGG | - | chr3.4:3643481-3643500 | None:intergenic | 40.0% |
| ! | CAGGTACTTGGTTGTTGATT+GGG | - | chr3.4:3643482-3643501 | None:intergenic | 40.0% |
| ! | CTTTTTCCAGTTGGGTTTGT+TGG | - | chr3.4:3643669-3643688 | None:intergenic | 40.0% |
| ! | GATAGAGTGAGTTGGATGTT+TGG | - | chr3.4:3643523-3643542 | None:intergenic | 40.0% |
| ! | GATTGGTAGTTTGGCATAGT+GGG | - | chr3.4:3643448-3643467 | None:intergenic | 40.0% |
| !! | TTGTTTGGTGGGTTACTTCT+TGG | - | chr3.4:3643573-3643592 | None:intergenic | 40.0% |
| ACCTGAATACTCAGCTGAGT+TGG | + | chr3.4:3643497-3643516 | MS.gene009420:CDS | 45.0% | |
| CAGCTGAGTATTCAGGTACT+TGG | - | chr3.4:3643494-3643513 | None:intergenic | 45.0% | |
| CGTATTGGAGGAACACATGA+AGG | + | chr3.4:3644100-3644119 | MS.gene009420:CDS | 45.0% | |
| TGAATCTGGTCACGTATTGG+AGG | + | chr3.4:3644088-3644107 | MS.gene009420:CDS | 45.0% | |
| ! | GGATTGGTAGTTTGGCATAG+TGG | - | chr3.4:3643449-3643468 | None:intergenic | 45.0% |
| ! | GGTACTTGGTTGTTGATTGG+GGG | - | chr3.4:3643480-3643499 | None:intergenic | 45.0% |
| ! | TCCTCCCAAAGCTTTTCCAT+TGG | - | chr3.4:3643415-3643434 | None:intergenic | 45.0% |
| !! | TCCAATGGAAAAGCTTTGGG+AGG | + | chr3.4:3643411-3643430 | MS.gene009420:CDS | 45.0% |
| !! | TGGTTGTTGATTGGGGGTTT+TGG | - | chr3.4:3643474-3643493 | None:intergenic | 45.0% |
| !!! | ATTGGGGGTTTTGGATGGAT+TGG | - | chr3.4:3643465-3643484 | None:intergenic | 45.0% |
| !!! | TGTTGATTGGGGGTTTTGGA+TGG | - | chr3.4:3643470-3643489 | None:intergenic | 45.0% |
| GCAGGAGAGATTGGTAGAAG+TGG | + | chr3.4:3644005-3644024 | MS.gene009420:CDS | 50.0% | |
| GCCAACTCAGCTGAGTATTC+AGG | - | chr3.4:3643501-3643520 | None:intergenic | 50.0% | |
| GTACCCCGTCAAACTTTGGT+AGG | + | chr3.4:3643622-3643641 | MS.gene009420:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 3643366 | 3644157 | 3643366 | ID=MS.gene009420 |
| chr3.4 | mRNA | 3643366 | 3644157 | 3643366 | ID=MS.gene009420.t1;Parent=MS.gene009420 |
| chr3.4 | exon | 3643366 | 3644157 | 3643366 | ID=MS.gene009420.t1.exon1;Parent=MS.gene009420.t1 |
| chr3.4 | CDS | 3643366 | 3644157 | 3643366 | ID=cds.MS.gene009420.t1;Parent=MS.gene009420.t1 |
| Gene Sequence |
| Protein sequence |
