AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009514


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009514.t1 MTR_4g086660 89.474 57 5 1 1 57 214 269 8.62e-29 108
MS.gene009514.t1 MTR_8g079790 82.456 57 9 1 1 57 205 260 5.70e-26 100
MS.gene009514.t1 MTR_3g092230 75.439 57 13 1 1 57 215 270 1.26e-24 96.7
MS.gene009514.t1 MTR_5g017830 77.193 57 12 1 1 57 211 266 1.55e-24 96.3
MS.gene009514.t1 MTR_7g118020 75.439 57 13 1 1 57 221 276 2.57e-24 95.5
MS.gene009514.t1 MTR_7g089480 75.439 57 13 1 1 57 218 273 8.36e-24 94.4
MS.gene009514.t1 MTR_5g022030 56.897 58 23 2 1 57 173 229 1.69e-14 67.8
MS.gene009514.t1 MTR_5g022030 56.897 58 23 2 1 57 173 229 1.70e-14 67.8
MS.gene009514.t1 MTR_5g092815 57.407 54 22 1 4 57 167 219 2.31e-14 67.4
MS.gene009514.t1 MTR_5g092810 55.556 54 23 1 4 57 168 220 4.08e-13 63.9
MS.gene009514.t1 MTR_1g026075 54.902 51 22 1 8 58 154 203 4.89e-13 62.4
MS.gene009514.t1 MTR_7g068710 60.000 50 19 1 8 57 136 184 1.05e-12 62.8
MS.gene009514.t1 MTR_1g026190 41.935 93 40 3 8 90 158 246 1.62e-12 62.0
MS.gene009514.t1 MTR_3g051770 53.704 54 24 1 4 57 160 212 1.71e-12 62.0
MS.gene009514.t1 MTR_7g091890 60.000 50 19 1 8 57 138 186 1.84e-12 62.0
MS.gene009514.t1 MTR_7g091890 60.000 50 19 1 8 57 138 186 1.90e-12 62.0
MS.gene009514.t1 MTR_1g052530 56.000 50 21 1 8 57 132 180 1.48e-11 59.3
MS.gene009514.t1 MTR_1g052530 56.000 50 21 1 8 57 132 180 1.52e-11 59.3
MS.gene009514.t1 MTR_4g107490 58.000 50 20 1 8 57 153 201 2.35e-11 58.9
MS.gene009514.t1 MTR_7g106710 52.632 57 25 2 1 57 209 263 6.06e-11 57.4
MS.gene009514.t1 MTR_7g106710 52.632 57 25 2 1 57 209 263 6.17e-11 57.4
MS.gene009514.t1 MTR_7g106710 52.632 57 25 2 1 57 209 263 6.24e-11 57.4
MS.gene009514.t1 MTR_7g106710 52.632 57 25 2 1 57 209 263 6.27e-11 57.4
MS.gene009514.t1 MTR_5g009830 52.727 55 25 1 8 62 147 200 9.58e-11 57.0
MS.gene009514.t1 MTR_5g009830 52.727 55 25 1 8 62 147 200 9.77e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009514.t1 AT3G50530 82.456 57 9 1 1 57 225 280 1.57e-26 102
MS.gene009514.t1 AT3G50530 82.456 57 9 1 1 57 225 280 1.61e-26 102
MS.gene009514.t1 AT3G19100 80.702 57 10 1 1 57 221 276 1.28e-25 99.4
MS.gene009514.t1 AT2G46700 77.193 57 12 1 1 57 39 94 6.62e-25 96.7
MS.gene009514.t1 AT2G46700 77.193 57 12 1 1 57 39 94 6.62e-25 96.7
MS.gene009514.t1 AT2G46700 77.193 57 12 1 1 57 220 275 1.22e-24 96.7
MS.gene009514.t1 AT2G41140 78.947 57 11 1 1 57 200 255 1.25e-24 96.7
MS.gene009514.t1 AT1G49580 84.000 50 8 0 8 57 233 282 2.25e-24 95.9
MS.gene009514.t1 AT3G56760 75.439 57 13 1 1 57 201 256 2.96e-23 92.8
MS.gene009514.t1 AT5G24430 72.222 54 15 0 4 57 222 275 1.65e-20 84.7
MS.gene009514.t1 AT3G49370 70.370 54 16 0 4 57 221 274 4.53e-20 83.6
MS.gene009514.t1 AT5G66210 58.621 58 22 2 1 57 136 192 3.08e-15 69.7
MS.gene009514.t1 AT5G66210 58.621 58 22 2 1 57 136 192 3.08e-15 69.7
MS.gene009514.t1 AT5G66210 58.621 58 22 2 1 57 136 192 3.16e-15 69.7
MS.gene009514.t1 AT5G66210 58.621 58 22 2 1 57 136 192 3.18e-15 69.7
MS.gene009514.t1 AT5G66210 58.621 58 22 2 1 57 136 192 3.21e-15 69.7
MS.gene009514.t1 AT5G66210 58.621 58 22 2 1 57 136 192 3.21e-15 69.7
MS.gene009514.t1 AT2G17890 58.621 58 22 2 1 57 182 238 8.33e-15 68.6
MS.gene009514.t1 AT4G36070 55.172 58 24 2 1 57 145 201 3.43e-14 67.0
MS.gene009514.t1 AT4G36070 55.172 58 24 2 1 57 145 201 3.44e-14 67.0
MS.gene009514.t1 AT5G12480 64.000 50 17 1 8 57 139 187 5.21e-14 66.2
MS.gene009514.t1 AT5G19450 64.000 50 17 1 8 57 137 185 9.62e-14 65.5
MS.gene009514.t1 AT5G19450 64.000 50 17 1 8 57 137 185 9.62e-14 65.5
MS.gene009514.t1 AT1G76040 58.000 50 20 1 8 57 192 240 6.15e-13 63.2
MS.gene009514.t1 AT1G76040 58.000 50 20 1 8 57 192 240 6.21e-13 63.2
MS.gene009514.t1 AT1G50700 60.000 50 19 1 8 57 153 201 1.05e-12 62.8
MS.gene009514.t1 AT1G50700 60.000 50 19 1 8 57 153 201 1.06e-12 62.8
MS.gene009514.t1 AT1G50700 60.000 50 19 1 8 57 153 201 1.07e-12 62.8
MS.gene009514.t1 AT3G20410 58.000 50 20 1 8 57 171 219 1.14e-12 62.4
MS.gene009514.t1 AT3G20410 58.000 50 20 1 8 57 171 219 1.14e-12 62.4
MS.gene009514.t1 AT2G31500 56.140 57 23 2 1 57 140 194 1.36e-12 62.4
MS.gene009514.t1 AT3G57530 60.000 50 19 1 8 57 143 191 1.72e-12 62.0
MS.gene009514.t1 AT3G57530 60.000 50 19 1 8 57 143 191 1.77e-12 62.0
MS.gene009514.t1 AT2G41860 58.000 50 20 1 8 57 134 182 2.21e-12 61.6
MS.gene009514.t1 AT2G41860 58.000 50 20 1 8 57 134 182 2.21e-12 61.6
MS.gene009514.t1 AT3G51850 58.000 50 20 1 8 57 134 182 3.19e-12 61.2
MS.gene009514.t1 AT3G51850 58.000 50 20 1 8 57 134 182 3.28e-12 61.2
MS.gene009514.t1 AT3G51850 58.000 50 20 1 8 57 134 182 3.30e-12 61.2
MS.gene009514.t1 AT4G04720 56.000 50 21 1 8 57 160 208 5.26e-12 60.8
MS.gene009514.t1 AT4G04720 56.000 50 21 1 8 57 160 208 5.26e-12 60.8
MS.gene009514.t1 AT1G74740 58.000 50 20 1 8 57 139 187 9.46e-12 60.1
MS.gene009514.t1 AT1G61950 54.000 50 22 1 8 57 178 226 1.20e-11 59.7
MS.gene009514.t1 AT4G04740 54.000 50 22 1 8 57 149 197 2.13e-11 58.9
MS.gene009514.t1 AT4G04740 54.000 50 22 1 8 57 149 197 2.13e-11 58.9
MS.gene009514.t1 AT4G04740 54.000 50 22 1 8 57 149 197 2.13e-11 58.9
MS.gene009514.t1 AT4G04740 54.000 50 22 1 8 57 149 197 2.13e-11 58.9
MS.gene009514.t1 AT4G04740 54.000 50 22 1 8 57 149 197 2.13e-11 58.9
MS.gene009514.t1 AT4G04740 54.000 50 22 1 8 57 149 197 2.13e-11 58.9
MS.gene009514.t1 AT4G04740 54.000 50 22 1 8 57 149 197 2.14e-11 58.9
MS.gene009514.t1 AT5G23580 58.000 50 20 1 8 57 102 150 2.21e-11 58.9
MS.gene009514.t1 AT4G21940 54.000 50 22 1 8 57 182 230 2.53e-11 58.5
MS.gene009514.t1 AT4G21940 54.000 50 22 1 8 57 182 230 2.56e-11 58.5
MS.gene009514.t1 AT4G21940 54.000 50 22 1 8 57 182 230 2.56e-11 58.5

Find 16 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAACAACAGACTGAAACT+TGG 0.474519 5.3:+62792271 None:intergenic
GACGTTTCAGGTTATGTGAA+GGG 0.515873 5.3:-62792748 MS.gene009514:intron
CACGATCCATGCACTTCTAT+CGG 0.521104 5.3:+62792229 None:intergenic
TATTTGTACCATGACAGCCT+TGG 0.549299 5.3:+62792469 None:intergenic
CAAGTTTCAGTCTGTTGTTG+AGG 0.553759 5.3:-62792270 MS.gene009514:CDS
AGATGATGCCAAGGCTGTCA+TGG 0.554908 5.3:-62792477 MS.gene009514:CDS
CGACGTTTCAGGTTATGTGA+AGG 0.570325 5.3:-62792749 MS.gene009514:intron
CTGAAACTTGGTGTAAGCAT+AGG 0.576927 5.3:+62792283 None:intergenic
ACGTTTCAGGTTATGTGAAG+GGG 0.578083 5.3:-62792747 MS.gene009514:intron
ATACTTAGAAGATGATGCCA+AGG 0.601527 5.3:-62792486 MS.gene009514:CDS
CGTTTCAGGTTATGTGAAGG+GGG 0.632420 5.3:-62792746 MS.gene009514:intron
GTTGAGGATAGCAGCAGATG+CGG 0.641502 5.3:-62792254 MS.gene009514:CDS
GCGGTACCGATAGAAGTGCA+TGG 0.643356 5.3:-62792235 MS.gene009514:CDS
TATTAGACATGATACTGTCA+AGG 0.648573 5.3:-62792718 MS.gene009514:intron
GCACAGAGATCTTAAGCCTG+AGG 0.658568 5.3:-62792408 MS.gene009514:intron
AAATGTATGTAGTGACACCA+AGG 0.670047 5.3:-62792870 MS.gene009514:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCATATTTAAATATCTTA+GGG - chr5.3:62792741-62792760 MS.gene009514:CDS 10.0%
!! TAATCATATTTAAATATCTT+AGG - chr5.3:62792740-62792759 MS.gene009514:CDS 10.0%
! TTAAACCTAATAAATCACCT+TGG + chr5.3:62792219-62792238 None:intergenic 25.0%
ATGATTTATTGCTTCTTCAG+AGG - chr5.3:62792554-62792573 MS.gene009514:intron 30.0%
TATTAGACATGATACTGTCA+AGG - chr5.3:62792351-62792370 MS.gene009514:intron 30.0%
TCTTTCATTTGTCTTTCAGA+TGG - chr5.3:62792692-62792711 MS.gene009514:intron 30.0%
TGAAGAAGCAATAAATCATG+TGG + chr5.3:62792553-62792572 None:intergenic 30.0%
ATACTTAGAAGATGATGCCA+AGG - chr5.3:62792583-62792602 MS.gene009514:intron 35.0%
ATTTATTGCTTCTTCAGAGG+AGG - chr5.3:62792557-62792576 MS.gene009514:intron 35.0%
TAAATCATGTGGCTAGTAAG+TGG + chr5.3:62792542-62792561 None:intergenic 35.0%
TGACACCAAGGTGATTTATT+AGG - chr5.3:62792211-62792230 MS.gene009514:CDS 35.0%
TTTATTGCTTCTTCAGAGGA+GGG - chr5.3:62792558-62792577 MS.gene009514:intron 35.0%
! GTTGCATTTTGTCATCTTCA+GGG - chr5.3:62792632-62792651 MS.gene009514:intron 35.0%
! TGTTGCATTTTGTCATCTTC+AGG - chr5.3:62792631-62792650 MS.gene009514:intron 35.0%
ATGAAAGATATGCTCACCTC+AGG + chr5.3:62792680-62792699 None:intergenic 40.0%
CAAGTTTCAGTCTGTTGTTG+AGG - chr5.3:62792799-62792818 MS.gene009514:intron 40.0%
CTCAACAACAGACTGAAACT+TGG + chr5.3:62792801-62792820 None:intergenic 40.0%
CTGAATACTATCGACGTTTC+AGG - chr5.3:62792309-62792328 MS.gene009514:CDS 40.0%
TATTTGTACCATGACAGCCT+TGG + chr5.3:62792603-62792622 None:intergenic 40.0%
! ATTTTGTCATCTTCAGGGTG+TGG - chr5.3:62792637-62792656 MS.gene009514:intron 40.0%
!! ACGTTTCAGGTTATGTGAAG+GGG - chr5.3:62792322-62792341 MS.gene009514:intron 40.0%
!! CTGAAACTTGGTGTAAGCAT+AGG + chr5.3:62792789-62792808 None:intergenic 40.0%
!! GACGTTTCAGGTTATGTGAA+GGG - chr5.3:62792321-62792340 MS.gene009514:intron 40.0%
CACGATCCATGCACTTCTAT+CGG + chr5.3:62792843-62792862 None:intergenic 45.0%
GCATGAAATAGAGCCGCTAT+CGG - chr5.3:62792394-62792413 MS.gene009514:intron 45.0%
TTGAAATAACGCCGCTAACG+CGG + chr5.3:62792488-62792507 None:intergenic 45.0%
!! CGACGTTTCAGGTTATGTGA+AGG - chr5.3:62792320-62792339 MS.gene009514:intron 45.0%
!! CGTTTCAGGTTATGTGAAGG+GGG - chr5.3:62792323-62792342 MS.gene009514:intron 45.0%
AGATGATGCCAAGGCTGTCA+TGG - chr5.3:62792592-62792611 MS.gene009514:intron 50.0%
CATGTGGCTAGTAAGTGGCA+AGG + chr5.3:62792537-62792556 None:intergenic 50.0%
GCACAGAGATCTTAAGCCTG+AGG - chr5.3:62792661-62792680 MS.gene009514:intron 50.0%
GTTGAGGATAGCAGCAGATG+CGG - chr5.3:62792815-62792834 MS.gene009514:intron 50.0%
!! GCGGTACCGATAGAAGTGCA+TGG - chr5.3:62792834-62792853 MS.gene009514:intron 55.0%
!! TGCTCACTTCTGGGGTGAGA+CGG + chr5.3:62792452-62792471 None:intergenic 55.0%
ATGGCCGCTGCTCACTTCTG+GGG + chr5.3:62792460-62792479 None:intergenic 60.0%
CTCACCCCAGAAGTGAGCAG+CGG - chr5.3:62792453-62792472 MS.gene009514:CDS 60.0%
GATGGCCGCTGCTCACTTCT+GGG + chr5.3:62792461-62792480 None:intergenic 60.0%
GGCCATCATAGCCGCGTTAG+CGG - chr5.3:62792474-62792493 MS.gene009514:CDS 60.0%
TGATGGCCGCTGCTCACTTC+TGG + chr5.3:62792462-62792481 None:intergenic 60.0%
TGGGGTGAGACGGTGCATGT+GGG + chr5.3:62792442-62792461 None:intergenic 60.0%
CATGTGGGGGAGGTCACCCA+CGG + chr5.3:62792427-62792446 None:intergenic 65.0%
CGCCGCTAACGCGGCTATGA+TGG + chr5.3:62792479-62792498 None:intergenic 65.0%
CTGGGGTGAGACGGTGCATG+TGG + chr5.3:62792443-62792462 None:intergenic 65.0%
GGGGTGAGACGGTGCATGTG+GGG + chr5.3:62792441-62792460 None:intergenic 65.0%
GGGTGAGACGGTGCATGTGG+GGG + chr5.3:62792440-62792459 None:intergenic 65.0%
TGAGACGGTGCATGTGGGGG+AGG + chr5.3:62792437-62792456 None:intergenic 65.0%
CGCTATCGGCGCAGTGCCGT+GGG - chr5.3:62792408-62792427 MS.gene009514:intron 70.0%
! CCACGGCACTGCGCCGATAG+CGG + chr5.3:62792410-62792429 None:intergenic 70.0%
CCGCTATCGGCGCAGTGCCG+TGG - chr5.3:62792407-62792426 MS.gene009514:intron 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 62792201 62792890 62792201 ID=MS.gene009514
chr5.3 mRNA 62792201 62792890 62792201 ID=MS.gene009514.t1;Parent=MS.gene009514
chr5.3 exon 62792871 62792890 62792871 ID=MS.gene009514.t1.exon1;Parent=MS.gene009514.t1
chr5.3 CDS 62792871 62792890 62792871 ID=cds.MS.gene009514.t1;Parent=MS.gene009514.t1
chr5.3 exon 62792719 62792760 62792719 ID=MS.gene009514.t1.exon2;Parent=MS.gene009514.t1
chr5.3 CDS 62792719 62792760 62792719 ID=cds.MS.gene009514.t1;Parent=MS.gene009514.t1
chr5.3 exon 62792409 62792517 62792409 ID=MS.gene009514.t1.exon3;Parent=MS.gene009514.t1
chr5.3 CDS 62792409 62792517 62792409 ID=cds.MS.gene009514.t1;Parent=MS.gene009514.t1
chr5.3 exon 62792201 62792329 62792201 ID=MS.gene009514.t1.exon4;Parent=MS.gene009514.t1
chr5.3 CDS 62792201 62792329 62792201 ID=cds.MS.gene009514.t1;Parent=MS.gene009514.t1
Gene Sequence

>MS.gene009514

ATGTATGTAGTGACACCAAGGTGATTTATTAGGTTTAACATTTATTTGTTGATTTGAATGCTTGCTGCTTGTGAGAATTTTTTGTTTATATAGCATATTCATAATTAACTGAATACTATCGACGTTTCAGGTTATGTGAAGGGGGAGAGCTATTAGACATGATACTGTCAAGGTAATTATTATGTTGTCAATAGCATGAAATAGAGCCGCTATCGGCGCAGTGCCGTGGGTGACCTCCCCCACATGCACCGTCTCACCCCAGAAGTGAGCAGCGGCCATCATAGCCGCGTTAGCGGCGTTATTTCAATAATAAAATTTGACTTAGTAATTGCACCTTGCCACTTACTAGCCACATGATTTATTGCTTCTTCAGAGGAGGGAAATACTTAGAAGATGATGCCAAGGCTGTCATGGTACAAATACTAAATGTTGTTGCATTTTGTCATCTTCAGGGTGTGGTGCACAGAGATCTTAAGCCTGAGGTGAGCATATCTTTCATTTGTCTTTCAGATGGTTAATGTCTTAGAGTCTGCTATAGTTAATCATATTTAAATATCTTAGGGTCATATTTCTCTGTTCTTGATGCCTATGCTTACACCAAGTTTCAGTCTGTTGTTGAGGATAGCAGCAGATGCGGTACCGATAGAAGTGCATGGATCGTGTTTGTGCAGTCAGATATATCTATTGTAA

Protein sequence

>MS.gene009514.t1

MYVVTPRLCEGGELLDMILSRGGKYLEDDAKAVMVQILNVVAFCHLQGVVHRDLKPEGHISLFLMPMLTPSFSLLLRIAADAVPIEVHGSCLCSQIYLL