AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009534

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009534.t1 MTR_5g076960 92.214 411 21 1 1 400 10 420 0.0 723
MS.gene009534.t1 MTR_5g076960 90.282 319 20 1 93 400 1 319 0.0 526
MS.gene009534.t1 MTR_3g064500 63.838 271 90 3 2 271 26 289 5.18e-103 309
MS.gene009534.t1 MTR_6g477860 36.889 450 196 15 15 398 38 465 8.71e-54 185
MS.gene009534.t1 MTR_1g067000 55.769 104 38 1 15 118 28 123 2.30e-34 129
MS.gene009534.t1 MTR_8g028655 38.863 211 94 6 23 217 53 244 1.28e-33 128
MS.gene009534.t1 MTR_7g118330 67.619 105 32 1 14 116 6 110 9.39e-33 130
MS.gene009534.t1 MTR_0123s0070 57.732 97 33 2 19 115 35 123 9.80e-31 119
MS.gene009534.t1 MTR_0123s0070 57.732 97 33 2 19 115 35 123 1.71e-30 119
MS.gene009534.t1 MTR_4g061370 61.053 95 33 1 165 255 332 426 3.35e-25 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009534.t1 AT5G17300 46.199 342 124 12 12 337 33 330 1.65e-55 187
MS.gene009534.t1 AT5G37260 58.537 164 59 3 14 177 16 170 2.27e-44 155
MS.gene009534.t1 AT3G10113 44.444 225 101 6 19 232 50 261 3.50e-37 138
MS.gene009534.t1 AT1G18330 41.909 241 109 7 19 232 35 271 5.54e-37 137
MS.gene009534.t1 AT1G18330 41.909 241 109 7 19 232 61 297 7.21e-37 138
MS.gene009534.t1 AT2G46830 61.798 89 34 0 11 99 1 89 1.70e-32 129
MS.gene009534.t1 AT3G09600 58.586 99 33 2 23 121 32 122 4.19e-32 123
MS.gene009534.t1 AT3G09600 58.586 99 33 2 23 121 32 122 4.19e-32 123
MS.gene009534.t1 AT3G09600 58.586 99 33 2 23 121 32 122 4.54e-32 123
MS.gene009534.t1 AT3G09600 58.586 99 33 2 23 121 32 122 4.54e-32 123
MS.gene009534.t1 AT3G09600 61.290 93 28 2 23 115 32 116 4.69e-32 122
MS.gene009534.t1 AT1G01060 64.486 107 36 1 11 115 1 107 1.22e-31 127
MS.gene009534.t1 AT1G01060 64.486 107 36 1 11 115 1 107 1.24e-31 127
MS.gene009534.t1 AT1G01060 64.486 107 36 1 11 115 1 107 1.24e-31 127
MS.gene009534.t1 AT1G01060 64.486 107 36 1 11 115 1 107 1.24e-31 127
MS.gene009534.t1 AT1G01060 64.486 107 36 1 11 115 1 107 1.24e-31 127
MS.gene009534.t1 AT1G01060 64.486 107 36 1 11 115 1 107 1.24e-31 127
MS.gene009534.t1 AT5G52660 56.122 98 35 1 19 116 57 146 1.16e-30 120
MS.gene009534.t1 AT5G52660 56.122 98 35 1 19 116 57 146 1.20e-30 120
MS.gene009534.t1 AT5G02840 51.304 115 47 3 23 136 37 143 3.79e-30 117
MS.gene009534.t1 AT1G01520 42.208 154 71 3 19 172 46 181 4.58e-30 117
MS.gene009534.t1 AT1G01520 42.208 154 71 3 19 172 46 181 4.58e-30 117
MS.gene009534.t1 AT5G02840 58.511 94 31 2 23 116 37 122 4.62e-30 117
MS.gene009534.t1 AT5G02840 58.511 94 31 2 23 116 37 122 4.62e-30 117
MS.gene009534.t1 AT4G01280 56.863 102 36 2 15 116 40 133 4.63e-30 118
MS.gene009534.t1 AT4G01280 56.863 102 36 2 15 116 40 133 4.87e-30 118
MS.gene009534.t1 AT5G02840 59.140 93 30 2 23 115 37 121 5.68e-30 117
MS.gene009534.t1 AT1G01520 42.208 154 71 3 19 172 46 181 5.88e-30 117
MS.gene009534.t1 AT1G01520 42.208 154 71 3 19 172 46 181 7.51e-30 117
MS.gene009534.t1 AT5G02840 51.304 115 47 3 23 136 37 143 8.06e-30 117
MS.gene009534.t1 AT5G17300 40.678 295 115 13 59 337 5 255 1.80e-23 100
MS.gene009534.t1 AT3G09600 50.000 64 24 1 52 115 5 60 4.81e-15 74.7
MS.gene009534.t1 AT3G09600 50.000 64 24 1 52 115 5 60 4.81e-15 74.7
MS.gene009534.t1 AT3G09600 50.000 64 24 1 52 115 5 60 5.40e-15 74.3
MS.gene009534.t1 AT3G09600 50.000 64 24 1 52 115 5 60 5.40e-15 74.3

Find 78 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGATGCACAACCTTTAA+TGG 0.191323 5.3:-62544511 MS.gene009534:intron
TCGCTTGTGTCGTTAGATTT+TGG 0.203212 5.3:-62544744 MS.gene009534:CDS
GATTTAAGAAATACCATTAA+AGG 0.226502 5.3:+62544498 None:intergenic
GTAATTCACATTATCACTTT+TGG 0.280046 5.3:+62543847 None:intergenic
TTCCTCAGATGATGATGATT+TGG 0.286247 5.3:+62544575 None:intergenic
TGGTTGCGACACATTCTTTA+GGG 0.316667 5.3:+62544200 None:intergenic
AAGGGTTTGTACCATATAAA+AGG 0.320529 5.3:-62543772 MS.gene009534:CDS
CTTGAAGCTTTAAAGTTATA+CGG 0.328100 5.3:-62545303 MS.gene009534:CDS
CTCCTTGTGCAGTGGCTTGA+AGG 0.345306 5.3:+62544009 None:intergenic
GGATGAATCGGCTTCCGTTT+TGG 0.347869 5.3:+62544837 None:intergenic
TTGGTTGCGACACATTCTTT+AGG 0.358230 5.3:+62544199 None:intergenic
ACAGTGAGACTTAAAACATT+TGG 0.363877 5.3:-62543797 MS.gene009534:CDS
CCTATTGGGAGGGAACTATA+AGG 0.389437 5.3:+62543676 None:intergenic
TGATGTTGAAGAAGACTTGC+AGG 0.391857 5.3:+62544105 None:intergenic
GTCTTGCAACTGTTGTTCCT+TGG 0.392635 5.3:-62544054 MS.gene009534:CDS
ATTGGTATTGATAGCACTAA+TGG 0.402949 5.3:+62544617 None:intergenic
AGACTTAAAACATTTGGAAA+AGG 0.403830 5.3:-62543791 MS.gene009534:CDS
TGAGACCGGGGACAAACTTC+CGG 0.418000 5.3:+62544641 None:intergenic
GTGGACTATTTCAGACAATT+CGG 0.423011 5.3:-62544032 MS.gene009534:CDS
CTCAAGAAGTCGAAAGCTAT+TGG 0.423039 5.3:+62544599 None:intergenic
CGTATAACTTTAAAGCTTCA+AGG 0.424014 5.3:+62545304 None:intergenic
GATAATGTGAATTACTTTGT+TGG 0.443037 5.3:-62543839 MS.gene009534:CDS
TTCTTCTGCTACTACTTCGT+TGG 0.453914 5.3:+62544180 None:intergenic
TTGCAACTGTTGTTCCTTGG+TGG 0.457649 5.3:-62544051 MS.gene009534:CDS
TTGTGCATCATGAGTTGAAC+CGG 0.461880 5.3:+62544521 None:intergenic
CCCTCGGGCTCCTTGTGCAG+TGG 0.465390 5.3:+62544001 None:intergenic
GCAGTTGCTTCAGAAATCCT+TGG 0.467989 5.3:-62544687 MS.gene009534:CDS
GTTGCAAGACTAGCAGGAAT+AGG 0.468495 5.3:+62544067 None:intergenic
ACTGCAGATAATACCGACTT+TGG 0.472597 5.3:+62544705 None:intergenic
GACTTAAAACATTTGGAAAA+GGG 0.481341 5.3:-62543790 MS.gene009534:CDS
TTACGTGGGTAGGGATGAAT+CGG 0.484669 5.3:+62544825 None:intergenic
TAAAGTTATACGGCCGAGCC+TGG 0.486225 5.3:-62545293 MS.gene009534:CDS
GAATGAAGGGAGAACCTTCA+AGG 0.489688 5.3:+62545493 None:intergenic
AAGGAAGGATCTTCTTGTGT+TGG 0.490240 5.3:-62543929 MS.gene009534:CDS
TGGAAATGACCATGCCTTGA+AGG 0.504748 5.3:-62545507 MS.gene009534:intron
CGGGGACAAACTTCCGGCAG+GGG 0.506435 5.3:+62544647 None:intergenic
ACAACAGTTGCAAGACTAGC+AGG 0.513968 5.3:+62544061 None:intergenic
CGAAGATAAAGAAATTCAAA+AGG 0.522634 5.3:-62543948 MS.gene009534:CDS
GATAAAGAAATTCAAAAGGA+AGG 0.531765 5.3:-62543944 MS.gene009534:CDS
GCGATTTCAACTACTTTACG+TGG 0.532217 5.3:+62544810 None:intergenic
ACCGGGGACAAACTTCCGGC+AGG 0.532872 5.3:+62544645 None:intergenic
TCAACTACTTTACGTGGGTA+GGG 0.535844 5.3:+62544816 None:intergenic
CTGTTTCACTTAGTGTTGTG+TGG 0.537415 5.3:+62543816 None:intergenic
GCCACTGCACAAGGAGCCCG+AGG 0.547161 5.3:-62544002 MS.gene009534:CDS
CCCTGCCGGAAGTTTGTCCC+CGG 0.547980 5.3:-62544646 MS.gene009534:CDS
CCTCAGTGGCTGTTCCAAGT+TGG 0.552497 5.3:+62544773 None:intergenic
AAGACTAGCAGGAATAGGTT+CGG 0.557239 5.3:+62544072 None:intergenic
TCTGATTCAGATACCCCTGC+CGG 0.559724 5.3:-62544660 MS.gene009534:CDS
TTCAACTACTTTACGTGGGT+AGG 0.562152 5.3:+62544815 None:intergenic
ATAGCACTAATGGATGAGAC+CGG 0.562200 5.3:+62544627 None:intergenic
TCACAAAACAGAGAGAAAAG+TGG 0.563691 5.3:-62545350 MS.gene009534:CDS
GGCCTTCAAGCCACTGCACA+AGG 0.565013 5.3:-62544011 MS.gene009534:CDS
CCGGGGACAAACTTCCGGCA+GGG 0.568889 5.3:+62544646 None:intergenic
CAAATAGACGAACTGAATGC+CGG 0.578213 5.3:-62544540 MS.gene009534:CDS
CAGATCAATCGAGACACTGA+TGG 0.591725 5.3:-62544906 MS.gene009534:CDS
TTCCACCTCCAAGACCAAAA+CGG 0.592397 5.3:-62544851 MS.gene009534:CDS
AAAGAATGAAATCTCCAACT+TGG 0.596383 5.3:-62544787 MS.gene009534:CDS
CAAGTCTTCTTCAACATCAA+CGG 0.596500 5.3:-62544101 MS.gene009534:CDS
TAGCACTAATGGATGAGACC+GGG 0.598406 5.3:+62544628 None:intergenic
CGACAGTAACAGATGAGAGG+AGG 0.603195 5.3:-62543721 MS.gene009534:CDS
AAGGGAGAACCTTCAAGGCA+TGG 0.608656 5.3:+62545498 None:intergenic
GACAGTAACAGATGAGAGGA+GGG 0.622093 5.3:-62543720 MS.gene009534:CDS
AGATCAATCGAGACACTGAT+GGG 0.631943 5.3:-62544905 MS.gene009534:CDS
GGAGGGAACATCAACGCATG+AGG 0.635432 5.3:-62543703 MS.gene009534:CDS
AGAAAACAACTCTCCAAAGT+CGG 0.638359 5.3:-62544718 MS.gene009534:CDS
GTCCAAATCATCATCATCTG+AGG 0.641348 5.3:-62544577 MS.gene009534:CDS
CGATTTCAACTACTTTACGT+GGG 0.649225 5.3:+62544811 None:intergenic
TATCTGAATCAGAGAAACCA+AGG 0.651306 5.3:+62544670 None:intergenic
TGTACCATATAAAAGGTGCA+TGG 0.659379 5.3:-62543765 MS.gene009534:CDS
GTTCGACAGTAACAGATGAG+AGG 0.674306 5.3:-62543724 MS.gene009534:CDS
CCACTGCACAAGGAGCCCGA+GGG 0.677376 5.3:-62544001 MS.gene009534:CDS
TGGGAATGACACAACAATGG+TGG 0.695952 5.3:-62544886 MS.gene009534:CDS
CCAACTTGGAACAGCCACTG+AGG 0.697656 5.3:-62544773 MS.gene009534:CDS
AGCACTAATGGATGAGACCG+GGG 0.712885 5.3:+62544629 None:intergenic
TTGTCTGAAATAGTCCACCA+AGG 0.714861 5.3:+62544037 None:intergenic
TGATGGGAATGACACAACAA+TGG 0.720056 5.3:-62544889 MS.gene009534:CDS
AAAAGTTTGAGAGTTCGACA+AGG 0.741043 5.3:+62544154 None:intergenic
ACAAGCGAATTAGACCTCAG+TGG 0.788179 5.3:+62544759 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATAAATCAGAAAATA+TGG - chr5.3:62544062-62544081 MS.gene009534:CDS 10.0%
!!! AAATTTATGTATACTTTTAT+AGG - chr5.3:62543854-62543873 MS.gene009534:CDS 10.0%
!!! ATAAATTTTCACTTAATATA+TGG + chr5.3:62544216-62544235 None:intergenic 10.0%
!!! ATTTTATATGCTTTGATTTT+TGG - chr5.3:62543797-62543816 MS.gene009534:CDS 15.0%
!! GATTTAAGAAATACCATTAA+AGG + chr5.3:62544750-62544769 None:intergenic 20.0%
!! TGCAATTAACAAAATGAAAT+AGG + chr5.3:62544777-62544796 None:intergenic 20.0%
!! TTAACAAAATGAAATAGGTT+AGG + chr5.3:62544772-62544791 None:intergenic 20.0%
!! TTCACTTAATATATGGTTTA+TGG + chr5.3:62544209-62544228 None:intergenic 20.0%
! AAGTGAAAATTTATGACGAA+TGG - chr5.3:62544222-62544241 MS.gene009534:intron 25.0%
! AGACTTAAAACATTTGGAAA+AGG - chr5.3:62545454-62545473 MS.gene009534:intron 25.0%
! AGATCAAACATAAGAACAAA+AGG + chr5.3:62544998-62545017 None:intergenic 25.0%
! CACAAATAGATAAGAATGAA+GGG + chr5.3:62543768-62543787 None:intergenic 25.0%
! CGAAGATAAAGAAATTCAAA+AGG - chr5.3:62545297-62545316 MS.gene009534:CDS 25.0%
! GACTTAAAACATTTGGAAAA+GGG - chr5.3:62545455-62545474 MS.gene009534:intron 25.0%
! GATAAAGAAATTCAAAAGGA+AGG - chr5.3:62545301-62545320 MS.gene009534:CDS 25.0%
! GATAATGTGAATTACTTTGT+TGG - chr5.3:62545406-62545425 MS.gene009534:intron 25.0%
! TCACAAATAGATAAGAATGA+AGG + chr5.3:62543769-62543788 None:intergenic 25.0%
! TCTTAATATTGTCTAGAAAC+TGG - chr5.3:62545015-62545034 MS.gene009534:intron 25.0%
!! GAAAATATGGTCTAAGTTTT+TGG - chr5.3:62544075-62544094 MS.gene009534:CDS 25.0%
!! GTAATTCACATTATCACTTT+TGG + chr5.3:62545401-62545420 None:intergenic 25.0%
!! TATGTATACTTTTATAGGCA+AGG - chr5.3:62543859-62543878 MS.gene009534:CDS 25.0%
!! TCATTTTGTTAATTGCAACA+AGG - chr5.3:62544780-62544799 MS.gene009534:CDS 25.0%
!!! AGTTTTGAATTTTCAGATCT+TGG - chr5.3:62543996-62544015 MS.gene009534:CDS 25.0%
!!! CTTGAAGCTTTAAAGTTATA+CGG - chr5.3:62543942-62543961 MS.gene009534:CDS 25.0%
AAAGAATGAAATCTCCAACT+TGG - chr5.3:62544458-62544477 MS.gene009534:intron 30.0%
AAAGATTCTTCTTGCAAAAG+AGG - chr5.3:62544032-62544051 MS.gene009534:CDS 30.0%
AAGGGTTTGTACCATATAAA+AGG - chr5.3:62545473-62545492 MS.gene009534:intron 30.0%
ACAGTGAGACTTAAAACATT+TGG - chr5.3:62545448-62545467 MS.gene009534:intron 30.0%
! CTACTTGATCAATTTTCATG+TGG - chr5.3:62543718-62543737 MS.gene009534:CDS 30.0%
! CTCTGTTTTGTGATAGTATA+TGG + chr5.3:62543888-62543907 None:intergenic 30.0%
!! ATTGGTATTGATAGCACTAA+TGG + chr5.3:62544631-62544650 None:intergenic 30.0%
!! CGTATAACTTTAAAGCTTCA+AGG + chr5.3:62543944-62543963 None:intergenic 30.0%
!!! ATTTTCAAGTTTCAACCTCA+AGG + chr5.3:62544274-62544293 None:intergenic 30.0%
!!! TGCTCAGAAGTTTTTTTCTA+AGG - chr5.3:62544158-62544177 MS.gene009534:CDS 30.0%
AAAAGTTTGAGAGTTCGACA+AGG + chr5.3:62545094-62545113 None:intergenic 35.0%
AGAAAACAACTCTCCAAAGT+CGG - chr5.3:62544527-62544546 MS.gene009534:CDS 35.0%
ATGTTCTGTGGTGAAAATGA+AGG + chr5.3:62544103-62544122 None:intergenic 35.0%
CAAGTCTTCTTCAACATCAA+CGG - chr5.3:62545144-62545163 MS.gene009534:intron 35.0%
CATGACCAATATCGTAATCA+AGG + chr5.3:62544959-62544978 None:intergenic 35.0%
CGATTTCAACTACTTTACGT+GGG + chr5.3:62544437-62544456 None:intergenic 35.0%
TAAGGCCTTGATTACGATAT+TGG - chr5.3:62544951-62544970 MS.gene009534:intron 35.0%
TATCTGAATCAGAGAAACCA+AGG + chr5.3:62544578-62544597 None:intergenic 35.0%
TCACAAAACAGAGAGAAAAG+TGG - chr5.3:62543895-62543914 MS.gene009534:CDS 35.0%
TCATGATGCACAACCTTTAA+TGG - chr5.3:62544734-62544753 MS.gene009534:CDS 35.0%
TTTGCATTTAGCATCTCCAA+GGG + chr5.3:62544248-62544267 None:intergenic 35.0%
! ATTTTCACCACAGAACATGT+TGG - chr5.3:62544105-62544124 MS.gene009534:CDS 35.0%
! GTGGACTATTTCAGACAATT+CGG - chr5.3:62545213-62545232 MS.gene009534:intron 35.0%
! TGAATGTAGATGTTTTCCCT+CGG + chr5.3:62545263-62545282 None:intergenic 35.0%
! TGTACCATATAAAAGGTGCA+TGG - chr5.3:62545480-62545499 MS.gene009534:intron 35.0%
! TGTCGAACTCTCAAACTTTT+TGG - chr5.3:62545094-62545113 MS.gene009534:CDS 35.0%
! TTCCTCAGATGATGATGATT+TGG + chr5.3:62544673-62544692 None:intergenic 35.0%
AAGACTAGCAGGAATAGGTT+CGG + chr5.3:62545176-62545195 None:intergenic 40.0%
AAGGAAGGATCTTCTTGTGT+TGG - chr5.3:62545316-62545335 MS.gene009534:CDS 40.0%
AATTTATGACGAATGGCCCT+TGG - chr5.3:62544229-62544248 MS.gene009534:intron 40.0%
AGATCAATCGAGACACTGAT+GGG - chr5.3:62544340-62544359 MS.gene009534:intron 40.0%
CAAATAGACGAACTGAATGC+CGG - chr5.3:62544705-62544724 MS.gene009534:CDS 40.0%
CTCAAGAAGTCGAAAGCTAT+TGG + chr5.3:62544649-62544668 None:intergenic 40.0%
CTGTTTCACTTAGTGTTGTG+TGG + chr5.3:62545432-62545451 None:intergenic 40.0%
GCGATTTCAACTACTTTACG+TGG + chr5.3:62544438-62544457 None:intergenic 40.0%
GCTAAATGCAAACTTCCTTG+AGG - chr5.3:62544256-62544275 MS.gene009534:intron 40.0%
GTCCAAATCATCATCATCTG+AGG - chr5.3:62544668-62544687 MS.gene009534:CDS 40.0%
GTTTGCATTTAGCATCTCCA+AGG + chr5.3:62544249-62544268 None:intergenic 40.0%
TCAACTACTTTACGTGGGTA+GGG + chr5.3:62544432-62544451 None:intergenic 40.0%
TGATGGGAATGACACAACAA+TGG - chr5.3:62544356-62544375 MS.gene009534:intron 40.0%
TGATGTTGAAGAAGACTTGC+AGG + chr5.3:62545143-62545162 None:intergenic 40.0%
TGCTAATGCAACTCCAATCA+TGG + chr5.3:62544880-62544899 None:intergenic 40.0%
TGGTTGCGACACATTCTTTA+GGG + chr5.3:62545048-62545067 None:intergenic 40.0%
TTCAACTACTTTACGTGGGT+AGG + chr5.3:62544433-62544452 None:intergenic 40.0%
TTCTTCTGCTACTACTTCGT+TGG + chr5.3:62545068-62545087 None:intergenic 40.0%
TTGGTTGCGACACATTCTTT+AGG + chr5.3:62545049-62545068 None:intergenic 40.0%
! AATACCTTCGATTTTTCGCC+AGG + chr5.3:62543973-62543992 None:intergenic 40.0%
! ATAGCACTAATGGATGAGAC+CGG + chr5.3:62544621-62544640 None:intergenic 40.0%
! GAATGTAGATGTTTTCCCTC+GGG + chr5.3:62545262-62545281 None:intergenic 40.0%
! TCTGCCATGCACCTTTTATA+TGG + chr5.3:62545487-62545506 None:intergenic 40.0%
! TTGTCTGAAATAGTCCACCA+AGG + chr5.3:62545211-62545230 None:intergenic 40.0%
! TTGTGCATCATGAGTTGAAC+CGG + chr5.3:62544727-62544746 None:intergenic 40.0%
!! ACTGCAGATAATACCGACTT+TGG + chr5.3:62544543-62544562 None:intergenic 40.0%
!! TCGCTTGTGTCGTTAGATTT+TGG - chr5.3:62544501-62544520 MS.gene009534:intron 40.0%
ACAACAGTTGCAAGACTAGC+AGG + chr5.3:62545187-62545206 None:intergenic 45.0%
ACAAGCGAATTAGACCTCAG+TGG + chr5.3:62544489-62544508 None:intergenic 45.0%
CAGATCAATCGAGACACTGA+TGG - chr5.3:62544339-62544358 MS.gene009534:intron 45.0%
CTTTGTGCCAACATGTTCTG+TGG + chr5.3:62544115-62544134 None:intergenic 45.0%
GAATGAAGGGAGAACCTTCA+AGG + chr5.3:62543755-62543774 None:intergenic 45.0%
GACAGTAACAGATGAGAGGA+GGG - chr5.3:62545525-62545544 MS.gene009534:CDS 45.0%
GCAGTTGCTTCAGAAATCCT+TGG - chr5.3:62544558-62544577 MS.gene009534:CDS 45.0%
GTCTTGCAACTGTTGTTCCT+TGG - chr5.3:62545191-62545210 MS.gene009534:intron 45.0%
GTTCGACAGTAACAGATGAG+AGG - chr5.3:62545521-62545540 MS.gene009534:CDS 45.0%
GTTGCAAGACTAGCAGGAAT+AGG + chr5.3:62545181-62545200 None:intergenic 45.0%
TGGAAATGACCATGCCTTGA+AGG - chr5.3:62543738-62543757 MS.gene009534:CDS 45.0%
TGGGAATGACACAACAATGG+TGG - chr5.3:62544359-62544378 MS.gene009534:intron 45.0%
TTACGTGGGTAGGGATGAAT+CGG + chr5.3:62544423-62544442 None:intergenic 45.0%
TTCCACCTCCAAGACCAAAA+CGG - chr5.3:62544394-62544413 MS.gene009534:intron 45.0%
TTGCAACTGTTGTTCCTTGG+TGG - chr5.3:62545194-62545213 MS.gene009534:intron 45.0%
! AAGGCCTTATGATGCAATCG+CGG + chr5.3:62544940-62544959 None:intergenic 45.0%
! TAGCACTAATGGATGAGACC+GGG + chr5.3:62544620-62544639 None:intergenic 45.0%
AAGGGAGAACCTTCAAGGCA+TGG + chr5.3:62543750-62543769 None:intergenic 50.0%
ACTCCAATCATGGTCGCGTT+AGG + chr5.3:62544870-62544889 None:intergenic 50.0%
CATCCTAACGCGACCATGAT+TGG - chr5.3:62544864-62544883 MS.gene009534:CDS 50.0%
CGACAGTAACAGATGAGAGG+AGG - chr5.3:62545524-62545543 MS.gene009534:CDS 50.0%
TAAAGTTATACGGCCGAGCC+TGG - chr5.3:62543952-62543971 MS.gene009534:CDS 50.0%
TCAGTCCATGATTGCAGTCG+CGG + chr5.3:62544820-62544839 None:intergenic 50.0%
TCTGATTCAGATACCCCTGC+CGG - chr5.3:62544585-62544604 MS.gene009534:CDS 50.0%
TGTCCCGCGATTGCATCATA+AGG - chr5.3:62544933-62544952 MS.gene009534:intron 50.0%
! AGCACTAATGGATGAGACCG+GGG + chr5.3:62544619-62544638 None:intergenic 50.0%
! AGGCCTTATGATGCAATCGC+GGG + chr5.3:62544939-62544958 None:intergenic 50.0%
! CTTCGATTTTTCGCCAGGCT+CGG + chr5.3:62543968-62543987 None:intergenic 50.0%
!! ATCGGCTTCCGTTTTGGTCT+TGG + chr5.3:62544405-62544424 None:intergenic 50.0%
!! GGATGAATCGGCTTCCGTTT+TGG + chr5.3:62544411-62544430 None:intergenic 50.0%
!!! TTCCGTTTTGGTCTTGGAGG+TGG + chr5.3:62544399-62544418 None:intergenic 50.0%
ACTGTCCGCGACTGCAATCA+TGG - chr5.3:62544812-62544831 MS.gene009534:CDS 55.0%
CCAACTTGGAACAGCCACTG+AGG - chr5.3:62544472-62544491 MS.gene009534:intron 55.0%
CCTCAGTGGCTGTTCCAAGT+TGG + chr5.3:62544475-62544494 None:intergenic 55.0%
CGAGCCTGGCGAAAAATCGA+AGG - chr5.3:62543966-62543985 MS.gene009534:CDS 55.0%
CTCCTTGTGCAGTGGCTTGA+AGG + chr5.3:62545239-62545258 None:intergenic 55.0%
GGAGGGAACATCAACGCATG+AGG - chr5.3:62545542-62545561 MS.gene009534:CDS 55.0%
TGAGACCGGGGACAAACTTC+CGG + chr5.3:62544607-62544626 None:intergenic 55.0%
!!! GGCTTCCGTTTTGGTCTTGG+AGG + chr5.3:62544402-62544421 None:intergenic 55.0%
GGCCTTCAAGCCACTGCACA+AGG - chr5.3:62545234-62545253 MS.gene009534:intron 60.0%
ACCGGGGACAAACTTCCGGC+AGG + chr5.3:62544603-62544622 None:intergenic 65.0%
CCACTGCACAAGGAGCCCGA+GGG - chr5.3:62545244-62545263 MS.gene009534:intron 65.0%
CCCTGCCGGAAGTTTGTCCC+CGG - chr5.3:62544599-62544618 MS.gene009534:CDS 65.0%
CCGGGGACAAACTTCCGGCA+GGG + chr5.3:62544602-62544621 None:intergenic 65.0%
CGGGGACAAACTTCCGGCAG+GGG + chr5.3:62544601-62544620 None:intergenic 65.0%
CCCTCGGGCTCCTTGTGCAG+TGG + chr5.3:62545247-62545266 None:intergenic 70.0%
GCCACTGCACAAGGAGCCCG+AGG - chr5.3:62545243-62545262 MS.gene009534:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 62543691 62545576 62543691 ID=MS.gene009534
chr5.3 mRNA 62543691 62545576 62543691 ID=MS.gene009534.t1;Parent=MS.gene009534
chr5.3 exon 62545508 62545576 62545508 ID=MS.gene009534.t1.exon1;Parent=MS.gene009534.t1
chr5.3 CDS 62545508 62545576 62545508 ID=cds.MS.gene009534.t1;Parent=MS.gene009534.t1
chr5.3 exon 62545280 62545391 62545280 ID=MS.gene009534.t1.exon2;Parent=MS.gene009534.t1
chr5.3 CDS 62545280 62545391 62545280 ID=cds.MS.gene009534.t1;Parent=MS.gene009534.t1
chr5.3 exon 62545088 62545149 62545088 ID=MS.gene009534.t1.exon3;Parent=MS.gene009534.t1
chr5.3 CDS 62545088 62545149 62545088 ID=cds.MS.gene009534.t1;Parent=MS.gene009534.t1
chr5.3 exon 62544512 62544925 62544512 ID=MS.gene009534.t1.exon4;Parent=MS.gene009534.t1
chr5.3 CDS 62544512 62544925 62544512 ID=cds.MS.gene009534.t1;Parent=MS.gene009534.t1
chr5.3 exon 62543691 62544236 62543691 ID=MS.gene009534.t1.exon5;Parent=MS.gene009534.t1
chr5.3 CDS 62543691 62544236 62543691 ID=cds.MS.gene009534.t1;Parent=MS.gene009534.t1
Gene Sequence

>MS.gene009534

ATGCATTCTGTTGTACATGTACAAAAACTACTTGATCAATTTTCATGTGGAAATGACCATGCCTTGAAGGTTCTCCCTTCATTCTTATCTATTTGTGAAATATTTAATTTTATATGCTTTGATTTTTGGACATTTTTGTGTCAATGTTACATATGTTTTAATCAAATTTATGTATACTTTTATAGGCAAGGAAACCATATACTATCACAAAACAGAGAGAAAAGTGGACAGATGAAGAACATAAGAAGTTCCTTGAAGCTTTAAAGTTATACGGCCGAGCCTGGCGAAAAATCGAAGGTATTACTAGTTTTGAATTTTCAGATCTTGGACAATTATATCATAAAGATTCTTCTTGCAAAAGAGGTTAAATTAATTAATAAATCAGAAAATATGGTCTAAGTTTTTGGATCCTTCATTTTCACCACAGAACATGTTGGCACAAAGACTGCTGTGCAGATTCGAAGTCATGCTCAGAAGTTTTTTTCTAAGGTTTATTGATTTATTCTTCTAATGCACCATAAACCATATATTAAGTGAAAATTTATGACGAATGGCCCTTGGAGATGCTAAATGCAAACTTCCTTGAGGTTGAAACTTGAAAATTTTATCTTGATTTCACTATTAACTCTTTTATTTCGACTATTTTAACAGATCAATCGAGACACTGATGGGAATGACACAACAATGGTGGAGACAATTGAAATTCCACCTCCAAGACCAAAACGGAAGCCGATTCATCCCTACCCACGTAAAGTAGTTGAAATCGCAAAGAATGAAATCTCCAACTTGGAACAGCCACTGAGGTCTAATTCGCTTGTGTCGTTAGATTTTGGTAAAGAAAACAACTCTCCAAAGTCGGTATTATCTGCAGTTGCTTCAGAAATCCTTGGTTTCTCTGATTCAGATACCCCTGCCGGAAGTTTGTCCCCGGTCTCATCCATTAGTGCTATCAATACCAATAGCTTTCGACTTCTTGAGTCCAAATCATCATCATCTGAGGAAGATCTGTCTCAACAAATAGACGAACTGAATGCCGGTTCAACTCATGATGCACAACCTTTAATGGTATTTCTTAAATCCTAACCTATTTCATTTTGTTAATTGCAACAAGGTTTTAAATAACTGTCCGCGACTGCAATCATGGACTGAACATCATAGTTTTTGATGTCTCAGCATCCTAACGCGACCATGATTGGAGTTGCATTAGCATATTTGATTGCAACTTTTTGCAATATCTAAAATTGTCCCGCGATTGCATCATAAGGCCTTGATTACGATATTGGTCATGAAAATATTTTTAAGATCCTTTTGTTCTTATGTTTGATCTTAATATTGTCTAGAAACTGGAGCTTTTCCCTAAAGAATGTGTCGCAACCAACGAAGTAGTAGCAGAAGAATCACCTTGTCGAACTCTCAAACTTTTTGGAACAACTTTGTTAGTAAAAGATACCTGCAAGTCTTCTTCAACATCAACGGACGCATCCGAACCTATTCCTGCTAGTCTTGCAACTGTTGTTCCTTGGTGGACTATTTCAGACAATTCGGCCTTCAAGCCACTGCACAAGGAGCCCGAGGGAAAACATCTACATTCAAATCATGAAGAATGCGAAGATAAAGAAATTCAAAAGGAAGGATCTTCTTGTGTTGGTTCTAATAGTACTAGTTCAATGAATGATGAAGAAAGCAATGAAAGATTCGATGATCAAGCCAAAAGTGATAATGTGAATTACTTTGTTGGCCACACAACACTAAGTGAAACAGTGAGACTTAAAACATTTGGAAAAGGGTTTGTACCATATAAAAGGTGCATGGCAGAAAGAGAACGACAATGTTCGACAGTAACAGATGAGAGGAGGGAACATCAACGCATGAGGCTTTCCTTATAG
Protein sequence

>MS.gene009534.t1

MHSVVHVQKLLDQFSCGNDHALKARKPYTITKQREKWTDEEHKKFLEALKLYGRAWRKIEEHVGTKTAVQIRSHAQKFFSKINRDTDGNDTTMVETIEIPPPRPKRKPIHPYPRKVVEIAKNEISNLEQPLRSNSLVSLDFGKENNSPKSVLSAVASEILGFSDSDTPAGSLSPVSSISAINTNSFRLLESKSSSSEEDLSQQIDELNAGSTHDAQPLMKLELFPKECVATNEVVAEESPCRTLKLFGTTLLVKDTCKSSSTSTDASEPIPASLATVVPWWTISDNSAFKPLHKEPEGKHLHSNHEECEDKEIQKEGSSCVGSNSTSSMNDEESNERFDDQAKSDNVNYFVGHTTLSETVRLKTFGKGFVPYKRCMAERERQCSTVTDERREHQRMRLSL