AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009635

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009635.t1 MTR_6g477950 80.247 81 16 0 234 314 64 144 5.93e-41 145
MS.gene009635.t1 MTR_5g071410 34.363 259 134 5 72 314 30 268 3.83e-29 112
MS.gene009635.t1 MTR_7g117585 42.000 100 58 0 213 312 44 143 2.03e-19 83.6
MS.gene009635.t1 MTR_8g069780 39.048 105 63 1 213 316 53 157 3.31e-19 83.2
MS.gene009635.t1 MTR_3g117130 45.122 82 45 0 235 316 199 280 1.99e-17 81.6
MS.gene009635.t1 MTR_2g437520 44.706 85 44 1 235 316 121 205 1.23e-15 74.7
MS.gene009635.t1 MTR_0131s0030 36.134 119 67 3 190 308 43 152 2.56e-14 70.1
MS.gene009635.t1 MTR_2g012700 29.412 102 70 1 213 314 20 119 4.95e-13 65.5
MS.gene009635.t1 MTR_6g018740 36.364 88 52 1 205 292 26 109 7.30e-12 62.8
MS.gene009635.t1 MTR_2g062330 30.392 102 68 2 213 314 40 138 3.29e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACAAAACATGGATTTGTT+AGG 0.260455 5.3:-60810760 MS.gene009635:CDS
GCAGAGTGTCTTGATTTCTT+AGG 0.270366 5.3:+60810786 None:intergenic
TGGACCAAGATAATAGAATT+TGG 0.305685 5.3:+60810625 None:intergenic
TGCTATGGCTCGGTCTATTA+CGG 0.344893 5.3:-60810354 MS.gene009635:CDS
ATCGCCAAATTCTATTATCT+TGG 0.357847 5.3:-60810629 MS.gene009635:CDS
CGAGCCATAGCAGCAGTAAC+AGG 0.380607 5.3:+60810365 None:intergenic
ATAATAGAATTTGGCGATAG+TGG 0.392649 5.3:+60810634 None:intergenic
AACAAAACATGGATTTGTTA+GGG 0.409075 5.3:-60810759 MS.gene009635:CDS
AGAAGCTAACAACGTGGAAT+TGG 0.411287 5.3:-60810558 MS.gene009635:CDS
GAACAAAGATGAGAAATCAT+TGG 0.429239 5.3:-60809747 MS.gene009635:CDS
GCTTCTTGTATGTTAGCATC+AGG 0.438291 5.3:+60810575 None:intergenic
AGAAGTGATTGACATTAGTT+CGG 0.445433 5.3:-60810471 MS.gene009635:CDS
TGACAAGCTTATATTTGAGT+TGG 0.448568 5.3:-60809678 MS.gene009635:CDS
AACAAAGATGAGAAATCATT+GGG 0.450109 5.3:-60809746 MS.gene009635:CDS
GCTATGGCTCGGTCTATTAC+GGG 0.456777 5.3:-60810353 MS.gene009635:CDS
CAGTGTCATCCATGGCTAAA+CGG 0.456837 5.3:+60810711 None:intergenic
TCCTACAGTTCTCTTTGTGA+AGG 0.461806 5.3:-60809648 MS.gene009635:CDS
AAGAGAGTACAATATGAATC+CGG 0.467615 5.3:+60812287 None:intergenic
GGAAGTTATTCAGATGGAAA+AGG 0.475791 5.3:-60810501 MS.gene009635:CDS
TGGAACTTGATAGAGAATAT+AGG 0.481727 5.3:-60809793 MS.gene009635:CDS
ACCAACGTCATCCACTTCCT+TGG 0.483119 5.3:+60809821 None:intergenic
GGTTCCTGTTACTGCTGCTA+TGG 0.489082 5.3:-60810369 MS.gene009635:CDS
ATCAAGTTCTCAAACAGAAA+AGG 0.489843 5.3:-60812479 MS.gene009635:intron
CTGTTACTGCTGCTATGGCT+CGG 0.498010 5.3:-60810364 MS.gene009635:CDS
GATGAAGAGGTACACATGAA+TGG 0.510552 5.3:-60810449 MS.gene009635:CDS
GTTAGGGATAATGAAGGTAG+AGG 0.514379 5.3:-60810743 MS.gene009635:CDS
TAATAGAATTTGGCGATAGT+GGG 0.516090 5.3:+60810635 None:intergenic
CTAACGAAGAACATGTTCAA+TGG 0.521649 5.3:+60810605 None:intergenic
ACACAATCCACAAGGTGTGC+AGG 0.526546 5.3:-60810522 MS.gene009635:CDS
GAATGGAGATGAAGAACAGC+AGG 0.527022 5.3:-60810432 MS.gene009635:CDS
TCTTTGTGAAGGTTGTGCCT+AGG 0.533902 5.3:-60809637 MS.gene009635:CDS
GATGAGAAATCATTGGGAAG+AGG 0.533916 5.3:-60809740 MS.gene009635:CDS
ATAACTTCCTGCACACCTTG+TGG 0.538431 5.3:+60810515 None:intergenic
TAAAGATCGCCGCCTAAAAG+AGG 0.538617 5.3:-60809702 MS.gene009635:CDS
GATTGTTATCCGTTTAGCCA+TGG 0.539405 5.3:-60810720 MS.gene009635:CDS
TGTGGATTGTGTAAAGCCTC+TGG 0.541269 5.3:+60810533 None:intergenic
TGCACTATTAAACTTATAAG+TGG 0.544607 5.3:+60812331 None:intergenic
AATCATTGGGAAGAGGATGG+TGG 0.546172 5.3:-60809733 MS.gene009635:CDS
GTGGATGACGTTGGTTGACA+TGG 0.546513 5.3:-60809813 MS.gene009635:CDS
ACCTCTCGAGAAACACGGTT+CGG 0.547753 5.3:+60809854 None:intergenic
TCCGAACCGTGTTTCTCGAG+AGG 0.547828 5.3:-60809855 MS.gene009635:CDS
GTCTATTACGGGACAACCAA+TGG 0.553763 5.3:-60810342 MS.gene009635:intron
TTAGGGATAATGAAGGTAGA+GGG 0.556265 5.3:-60810742 MS.gene009635:CDS
GGATTTGTTAGGGATAATGA+AGG 0.556809 5.3:-60810749 MS.gene009635:CDS
TGACATTAGTTCGGATGAAG+AGG 0.557403 5.3:-60810462 MS.gene009635:CDS
AGAAATCATTGGGAAGAGGA+TGG 0.573360 5.3:-60809736 MS.gene009635:CDS
GGAAACTCCAGTGTCATCCA+TGG 0.592097 5.3:+60810703 None:intergenic
CGTTTAGCCATGGATGACAC+TGG 0.595812 5.3:-60810710 MS.gene009635:CDS
GGATGAAGTTGTGCAAGAAG+AGG 0.599498 5.3:-60810411 MS.gene009635:CDS
ACTCTGCAATTAACAAAACA+TGG 0.609024 5.3:-60810770 MS.gene009635:CDS
TGTGCAGGAAGTTATTCAGA+TGG 0.612533 5.3:-60810507 MS.gene009635:CDS
TTCACAAAGAGAACTGTAGG+AGG 0.613714 5.3:+60809650 None:intergenic
AAAGATCGCCGCCTAAAAGA+GGG 0.615542 5.3:-60809701 MS.gene009635:CDS
TTACAACTATCAGATGAAGA+AGG 0.615627 5.3:-60812625 None:intergenic
CAACGTGGAATTGGTGCCAG+AGG 0.615652 5.3:-60810549 MS.gene009635:CDS
ACCTTCACAAAGAGAACTGT+AGG 0.621781 5.3:+60809647 None:intergenic
AATTAAGGCACCGTTCCTCA+TGG 0.623938 5.3:+60810682 None:intergenic
TCAAAACCTCTCGAGAAACA+CGG 0.634989 5.3:+60809849 None:intergenic
CCTCAACCAACATTCTACCT+AGG 0.635514 5.3:+60809620 None:intergenic
AATAGAATTTGGCGATAGTG+GGG 0.637122 5.3:+60810636 None:intergenic
GCAAGCTAAACATCGCTGAA+CGG 0.653140 5.3:-60809769 MS.gene009635:CDS
ACTGGAGTTTCCATGAGGAA+CGG 0.668736 5.3:-60810692 MS.gene009635:CDS
TAACAGGAACCTCAAAACGA+TGG 0.671726 5.3:+60810381 None:intergenic
TCACAAAGAGAACTGTAGGA+GGG 0.690868 5.3:+60809651 None:intergenic
ACCAAGGAAGTGGATGACGT+TGG 0.708377 5.3:-60809822 MS.gene009635:CDS
CATACAAGAAGCTAACAACG+TGG 0.731918 5.3:-60810564 MS.gene009635:CDS
GAGGCTTTACACAATCCACA+AGG 0.741516 5.3:-60810530 MS.gene009635:CDS
ATGACACTGGAGTTTCCATG+AGG 0.771808 5.3:-60810697 MS.gene009635:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATACTTAACTATAAA+AGG - chr5.3:60810837-60810856 MS.gene009635:intron 10.0%
!!! AAAAATTAAAACACTTTTTA+AGG + chr5.3:60810930-60810949 None:intergenic 10.0%
!!! TTATTTTTTATTCTTATTTG+TGG - chr5.3:60809793-60809812 MS.gene009635:CDS 10.0%
!! TAATTCAAAGTATATCTAAT+TGG - chr5.3:60812219-60812238 MS.gene009635:intron 15.0%
!! TACATTTGATTAAAATTGTA+TGG - chr5.3:60812325-60812344 MS.gene009635:CDS 15.0%
!! TATCTTCTTTATAATTCATT+CGG - chr5.3:60811952-60811971 MS.gene009635:intron 15.0%
!!! AAGTTATTTTACTTTTATGT+TGG - chr5.3:60812359-60812378 MS.gene009635:CDS 15.0%
!!! AATTAATTTTAGTTCAAATG+TGG - chr5.3:60812179-60812198 MS.gene009635:intron 15.0%
!!! AGGTTTTATGTTTAAATATA+TGG - chr5.3:60809720-60809739 MS.gene009635:CDS 15.0%
!!! GTTAAATTTCATTTTTTGTT+TGG - chr5.3:60811320-60811339 MS.gene009635:intron 15.0%
!!! TCATTTCTGATTTTTTTTTT+TGG - chr5.3:60810674-60810693 MS.gene009635:CDS 15.0%
!!! TTTTGATAATTAATTGTAGT+AGG + chr5.3:60810107-60810126 None:intergenic 15.0%
!! AAATGTCTACAATTAATTCA+AGG + chr5.3:60811141-60811160 None:intergenic 20.0%
!! AACAAAATTATGAGTGAAAA+AGG + chr5.3:60810583-60810602 None:intergenic 20.0%
!! AATATTATACTCAAAGAAGT+TGG - chr5.3:60810290-60810309 MS.gene009635:intron 20.0%
!! ACAAAATTATGAGTGAAAAA+GGG + chr5.3:60810582-60810601 None:intergenic 20.0%
!! ATATTTATTTGACCAAGTTA+TGG + chr5.3:60810255-60810274 None:intergenic 20.0%
!! GTATAAAACATAAATAACCA+AGG + chr5.3:60812290-60812309 None:intergenic 20.0%
!! TGAATTATAAAGAAGATAGA+AGG + chr5.3:60811950-60811969 None:intergenic 20.0%
!! TTGAATTAATTGTAGACATT+TGG - chr5.3:60811140-60811159 MS.gene009635:intron 20.0%
!!! ATATTGTACTTTTAGTAAAC+CGG - chr5.3:60809940-60809959 MS.gene009635:intron 20.0%
!!! TTTTGATATTATACCATTGT+TGG - chr5.3:60810946-60810965 MS.gene009635:intron 20.0%
!!! TTTTTTGTATATCTTACCAT+TGG + chr5.3:60811923-60811942 None:intergenic 20.0%
! AACAAAGATGAGAAATCATT+GGG - chr5.3:60812500-60812519 MS.gene009635:CDS 25.0%
! AAGATCTATATTCCATAACT+TGG - chr5.3:60810240-60810259 MS.gene009635:intron 25.0%
! AATTAATTGTAGACATTTGG+AGG - chr5.3:60811143-60811162 MS.gene009635:intron 25.0%
! AGTATACATTGTTATACATG+AGG - chr5.3:60811297-60811316 MS.gene009635:intron 25.0%
! ATTAATTGTAGACATTTGGA+GGG - chr5.3:60811144-60811163 MS.gene009635:intron 25.0%
! CCTAAAAGCAACAAAATAAA+TGG + chr5.3:60811412-60811431 None:intergenic 25.0%
! CGATTGAAAAAGGTAAATAA+TGG + chr5.3:60809862-60809881 None:intergenic 25.0%
! CTTAACTATAAAAGGAATAG+AGG - chr5.3:60810845-60810864 MS.gene009635:intron 25.0%
! GTATACATTGTTATACATGA+GGG - chr5.3:60811298-60811317 MS.gene009635:intron 25.0%
! GTATATAGCAAATCAATGTT+TGG - chr5.3:60810329-60810348 MS.gene009635:intron 25.0%
! TGCACTATTAAACTTATAAG+TGG + chr5.3:60809918-60809937 None:intergenic 25.0%
!! ACATTTTTGCTTGGTTTATT+GGG + chr5.3:60810383-60810402 None:intergenic 25.0%
!! ATATACCCTTTTTCCAAAAT+CGG + chr5.3:60810782-60810801 None:intergenic 25.0%
!! ATTGGATTTTCGAAATTGAA+GGG + chr5.3:60810612-60810631 None:intergenic 25.0%
!! CTTTTCAATATTAAACTCTC+TGG + chr5.3:60809894-60809913 None:intergenic 25.0%
!! TAACAAAACATGGATTTGTT+AGG - chr5.3:60811486-60811505 MS.gene009635:intron 25.0%
!! TGGTTTGTAATCTTAGATTA+AGG - chr5.3:60809813-60809832 MS.gene009635:CDS 25.0%
!!! AACAAAACATGGATTTGTTA+GGG - chr5.3:60811487-60811506 MS.gene009635:intron 25.0%
!!! ATTTCATTTTTTGTTTGGTG+CGG - chr5.3:60811325-60811344 MS.gene009635:intron 25.0%
!!! CCATTTATTTTGTTGCTTTT+AGG - chr5.3:60811409-60811428 MS.gene009635:intron 25.0%
!!! TATATCCGATTTTGGAAAAA+GGG - chr5.3:60810774-60810793 MS.gene009635:CDS 25.0%
!!! TTTTTGGAATTTCGCTTAAT+AGG - chr5.3:60810690-60810709 MS.gene009635:CDS 25.0%
AAATGAATGTGCAAGCATAT+AGG + chr5.3:60810660-60810679 None:intergenic 30.0%
ACTCTGCAATTAACAAAACA+TGG - chr5.3:60811476-60811495 MS.gene009635:intron 30.0%
AGAAGTGATTGACATTAGTT+CGG - chr5.3:60811775-60811794 MS.gene009635:intron 30.0%
ATAAACCAAGCAAAAATGTG+TGG - chr5.3:60810384-60810403 MS.gene009635:CDS 30.0%
ATAATAGAATTTGGCGATAG+TGG + chr5.3:60811615-60811634 None:intergenic 30.0%
ATCAAGTTCTCAAACAGAAA+AGG - chr5.3:60809767-60809786 MS.gene009635:CDS 30.0%
ATCGCCAAATTCTATTATCT+TGG - chr5.3:60811617-60811636 MS.gene009635:intron 30.0%
CAGGAAAATATAACCAACAA+TGG + chr5.3:60810962-60810981 None:intergenic 30.0%
GAACAAAGATGAGAAATCAT+TGG - chr5.3:60812499-60812518 MS.gene009635:CDS 30.0%
GCAAGTGTTTCATTAGATTT+GGG - chr5.3:60811250-60811269 MS.gene009635:intron 30.0%
GCCTTAATTGCTAAAAAGTT+TGG - chr5.3:60811578-60811597 MS.gene009635:intron 30.0%
GGATCATGAACAAACATAAA+GGG + chr5.3:60812110-60812129 None:intergenic 30.0%
TAATAGAATTTGGCGATAGT+GGG + chr5.3:60811614-60811633 None:intergenic 30.0%
TATGTTTGTTCATGATCCAT+AGG - chr5.3:60812112-60812131 MS.gene009635:intron 30.0%
TGACAAGCTTATATTTGAGT+TGG - chr5.3:60812568-60812587 MS.gene009635:intron 30.0%
TGCAAGTGTTTCATTAGATT+TGG - chr5.3:60811249-60811268 MS.gene009635:intron 30.0%
TGGAACTTGATAGAGAATAT+AGG - chr5.3:60812453-60812472 MS.gene009635:intron 30.0%
TGGACCAAGATAATAGAATT+TGG + chr5.3:60811624-60811643 None:intergenic 30.0%
TGGATCATGAACAAACATAA+AGG + chr5.3:60812111-60812130 None:intergenic 30.0%
TTAAAAAATGCACCTACCTA+TGG + chr5.3:60812131-60812150 None:intergenic 30.0%
! AAGAGAGTACAATATGAATC+CGG + chr5.3:60809962-60809981 None:intergenic 30.0%
! ATGAGTGAAAAAGGGTATAA+AGG + chr5.3:60810574-60810593 None:intergenic 30.0%
! CACATTTTTGCTTGGTTTAT+TGG + chr5.3:60810384-60810403 None:intergenic 30.0%
! CATTGGATTTTCGAAATTGA+AGG + chr5.3:60810613-60810632 None:intergenic 30.0%
! TCATTCAGTTTAAAGCTTAC+AGG + chr5.3:60810981-60811000 None:intergenic 30.0%
! TTGGATTTTCGAAATTGAAG+GGG + chr5.3:60810611-60810630 None:intergenic 30.0%
! TTTTGCAGTATATCCGATTT+TGG - chr5.3:60810766-60810785 MS.gene009635:CDS 30.0%
!! CAAAGAAGTTGGTATTTAAG+TGG - chr5.3:60810301-60810320 MS.gene009635:intron 30.0%
!! GCCAAACTTTTTAGCAATTA+AGG + chr5.3:60811582-60811601 None:intergenic 30.0%
!! GTATATCCGATTTTGGAAAA+AGG - chr5.3:60810773-60810792 MS.gene009635:CDS 30.0%
!!! AACATGTTTTGTTTTTGTGC+AGG - chr5.3:60809700-60809719 MS.gene009635:CDS 30.0%
!!! GTAGGTGCATTTTTTAAGTT+TGG - chr5.3:60812134-60812153 MS.gene009635:intron 30.0%
!!! TATTTACCTTTTTCAATCGC+AGG - chr5.3:60809863-60809882 MS.gene009635:CDS 30.0%
AATAGAATTTGGCGATAGTG+GGG + chr5.3:60811613-60811632 None:intergenic 35.0%
CTAACGAAGAACATGTTCAA+TGG + chr5.3:60811644-60811663 None:intergenic 35.0%
GAAGCGCATATAACAAAATG+TGG + chr5.3:60812035-60812054 None:intergenic 35.0%
GGAAGTTATTCAGATGGAAA+AGG - chr5.3:60811745-60811764 MS.gene009635:intron 35.0%
TAGGAAAAACTCGAAACTCA+CGG + chr5.3:60811444-60811463 None:intergenic 35.0%
TATAAAGGATGTCGTACACT+TGG + chr5.3:60810559-60810578 None:intergenic 35.0%
TTAGGGATAATGAAGGTAGA+GGG - chr5.3:60811504-60811523 MS.gene009635:intron 35.0%
TTTGTTCATGATCCATAGGT+AGG - chr5.3:60812116-60812135 MS.gene009635:intron 35.0%
! GTTGGTAGAAAGTATATGCA+AGG + chr5.3:60810438-60810457 None:intergenic 35.0%
!! GGATTTGTTAGGGATAATGA+AGG - chr5.3:60811497-60811516 MS.gene009635:intron 35.0%
!!! CAGGGATTTTTTCCTAACTA+TGG - chr5.3:60811167-60811186 MS.gene009635:intron 35.0%
!!! TTTTGAAACAACCAAGGAAG+TGG - chr5.3:60812414-60812433 MS.gene009635:intron 35.0%
ACCTTCACAAAGAGAACTGT+AGG + chr5.3:60812602-60812621 None:intergenic 40.0%
AGAAATCATTGGGAAGAGGA+TGG - chr5.3:60812510-60812529 MS.gene009635:CDS 40.0%
AGAAGCTAACAACGTGGAAT+TGG - chr5.3:60811688-60811707 MS.gene009635:intron 40.0%
AGGAAAAACTCGAAACTCAC+GGG + chr5.3:60811443-60811462 None:intergenic 40.0%
ATAGGACTTGCGATTTGCAA+GGG + chr5.3:60810642-60810661 None:intergenic 40.0%
CATACAAGAAGCTAACAACG+TGG - chr5.3:60811682-60811701 MS.gene009635:intron 40.0%
GATGAGAAATCATTGGGAAG+AGG - chr5.3:60812506-60812525 MS.gene009635:CDS 40.0%
GATGAGTGTGATTGTGTTTC+AGG + chr5.3:60810469-60810488 None:intergenic 40.0%
GATTGTTATCCGTTTAGCCA+TGG - chr5.3:60811526-60811545 MS.gene009635:intron 40.0%
GATTTCAATGGCGAAGGAAA+TGG + chr5.3:60810491-60810510 None:intergenic 40.0%
GCAATACCTGCGATTGAAAA+AGG + chr5.3:60809872-60809891 None:intergenic 40.0%
GCTTCTTGTATGTTAGCATC+AGG + chr5.3:60811674-60811693 None:intergenic 40.0%
GTTAGGGATAATGAAGGTAG+AGG - chr5.3:60811503-60811522 MS.gene009635:intron 40.0%
TAACAGGAACCTCAAAACGA+TGG + chr5.3:60811868-60811887 None:intergenic 40.0%
TAGTGTGTACATACCTCTTC+CGG + chr5.3:60811198-60811217 None:intergenic 40.0%
TATAGGACTTGCGATTTGCA+AGG + chr5.3:60810643-60810662 None:intergenic 40.0%
TCAAAACCTCTCGAGAAACA+CGG + chr5.3:60812400-60812419 None:intergenic 40.0%
TCACAAAGAGAACTGTAGGA+GGG + chr5.3:60812598-60812617 None:intergenic 40.0%
TCCTACAGTTCTCTTTGTGA+AGG - chr5.3:60812598-60812617 MS.gene009635:intron 40.0%
TGACATTAGTTCGGATGAAG+AGG - chr5.3:60811784-60811803 MS.gene009635:intron 40.0%
TGTGCAGGAAGTTATTCAGA+TGG - chr5.3:60811739-60811758 MS.gene009635:intron 40.0%
TTCACAAAGAGAACTGTAGG+AGG + chr5.3:60812599-60812618 None:intergenic 40.0%
TTTCCTAACTATGGTGAACC+CGG - chr5.3:60811176-60811195 MS.gene009635:intron 40.0%
! AACAGCCACACATTTTTGCT+TGG + chr5.3:60810392-60810411 None:intergenic 40.0%
! AAGGGTATATGCACGAATAG+AGG - chr5.3:60810792-60810811 MS.gene009635:CDS 40.0%
! GATGAAGAGGTACACATGAA+TGG - chr5.3:60811797-60811816 MS.gene009635:intron 40.0%
! GCAGAGTGTCTTGATTTCTT+AGG + chr5.3:60811463-60811482 None:intergenic 40.0%
! GGAAAATCTCCATCGTTTTG+AGG - chr5.3:60811856-60811875 MS.gene009635:intron 40.0%
! GTGTTTCAGGTGAAAAACGT+TGG + chr5.3:60810456-60810475 None:intergenic 40.0%
! TAAGCTTGTCACCCTCTTTT+AGG + chr5.3:60812559-60812578 None:intergenic 40.0%
!!! GAGAGGTTTTGAAACAACCA+AGG - chr5.3:60812408-60812427 MS.gene009635:intron 40.0%
AAAGATCGCCGCCTAAAAGA+GGG - chr5.3:60812545-60812564 MS.gene009635:intron 45.0%
AATCATTGGGAAGAGGATGG+TGG - chr5.3:60812513-60812532 MS.gene009635:CDS 45.0%
ACTGGAGTTTCCATGAGGAA+CGG - chr5.3:60811554-60811573 MS.gene009635:intron 45.0%
AGAGGTCCATGCAAGATTAG+AGG - chr5.3:60810863-60810882 MS.gene009635:intron 45.0%
AGTGTGTACATACCTCTTCC+GGG + chr5.3:60811197-60811216 None:intergenic 45.0%
ATAACTTCCTGCACACCTTG+TGG + chr5.3:60811734-60811753 None:intergenic 45.0%
ATGACACTGGAGTTTCCATG+AGG - chr5.3:60811549-60811568 MS.gene009635:intron 45.0%
ATTGTAGACATTTGGAGGGC+AGG - chr5.3:60811148-60811167 MS.gene009635:intron 45.0%
ATTTGCAAGGGGACATGCAT+TGG + chr5.3:60810630-60810649 None:intergenic 45.0%
CAAGATGTCACATAGTGCCT+TGG - chr5.3:60812270-60812289 MS.gene009635:CDS 45.0%
CAGTGTCATCCATGGCTAAA+CGG + chr5.3:60811538-60811557 None:intergenic 45.0%
GAATGGAGATGAAGAACAGC+AGG - chr5.3:60811814-60811833 MS.gene009635:intron 45.0%
GAGGCTTTACACAATCCACA+AGG - chr5.3:60811716-60811735 MS.gene009635:intron 45.0%
GCAAGCTAAACATCGCTGAA+CGG - chr5.3:60812477-60812496 MS.gene009635:intron 45.0%
GCATGTCCTCTAATCTTGCA+TGG + chr5.3:60810872-60810891 None:intergenic 45.0%
GGATGAAGTTGTGCAAGAAG+AGG - chr5.3:60811835-60811854 MS.gene009635:intron 45.0%
GTCTATTACGGGACAACCAA+TGG - chr5.3:60811904-60811923 MS.gene009635:intron 45.0%
TAAAGATCGCCGCCTAAAAG+AGG - chr5.3:60812544-60812563 MS.gene009635:intron 45.0%
TAGGACTTGCGATTTGCAAG+GGG + chr5.3:60810641-60810660 None:intergenic 45.0%
TCTTTGTGAAGGTTGTGCCT+AGG - chr5.3:60812609-60812628 MS.gene009635:intron 45.0%
TGAAATCGCAAGCGTGCAAA+GGG - chr5.3:60810504-60810523 MS.gene009635:CDS 45.0%
TTGAAATCGCAAGCGTGCAA+AGG - chr5.3:60810503-60810522 MS.gene009635:CDS 45.0%
TTGCACGCTTGCGATTTCAA+TGG + chr5.3:60810503-60810522 None:intergenic 45.0%
TTGTAGACATTTGGAGGGCA+GGG - chr5.3:60811149-60811168 MS.gene009635:intron 45.0%
! TGTGGATTGTGTAAAGCCTC+TGG + chr5.3:60811716-60811735 None:intergenic 45.0%
!! AATTAAGGCACCGTTCCTCA+TGG + chr5.3:60811567-60811586 None:intergenic 45.0%
!! TGCTATGGCTCGGTCTATTA+CGG - chr5.3:60811892-60811911 MS.gene009635:intron 45.0%
!!! TTTTTGTTTGGTGCGGCGAT+AGG - chr5.3:60811332-60811351 MS.gene009635:intron 45.0%
AAATCGCAAGCGTGCAAAGG+GGG - chr5.3:60810506-60810525 MS.gene009635:CDS 50.0%
AACTATGGTGAACCCGGAAG+AGG - chr5.3:60811182-60811201 MS.gene009635:intron 50.0%
ACACAATCCACAAGGTGTGC+AGG - chr5.3:60811724-60811743 MS.gene009635:intron 50.0%
ACCAACGTCATCCACTTCCT+TGG + chr5.3:60812428-60812447 None:intergenic 50.0%
ACCAAGGAAGTGGATGACGT+TGG - chr5.3:60812424-60812443 MS.gene009635:intron 50.0%
ACCTCTCGAGAAACACGGTT+CGG + chr5.3:60812395-60812414 None:intergenic 50.0%
CGTTTAGCCATGGATGACAC+TGG - chr5.3:60811536-60811555 MS.gene009635:intron 50.0%
CTGTTACTGCTGCTATGGCT+CGG - chr5.3:60811882-60811901 MS.gene009635:intron 50.0%
CTTCCGGGTTCACCATAGTT+AGG + chr5.3:60811182-60811201 None:intergenic 50.0%
GAAATCGCAAGCGTGCAAAG+GGG - chr5.3:60810505-60810524 MS.gene009635:CDS 50.0%
GCTTGCGATTTCAATGGCGA+AGG + chr5.3:60810497-60810516 None:intergenic 50.0%
GGAAACTCCAGTGTCATCCA+TGG + chr5.3:60811546-60811565 None:intergenic 50.0%
GGTTCCTGTTACTGCTGCTA+TGG - chr5.3:60811877-60811896 MS.gene009635:intron 50.0%
! GCTTGTCACCCTCTTTTAGG+CGG + chr5.3:60812556-60812575 None:intergenic 50.0%
! GTGGATGACGTTGGTTGACA+TGG - chr5.3:60812433-60812452 MS.gene009635:intron 50.0%
!! GCTATGGCTCGGTCTATTAC+GGG - chr5.3:60811893-60811912 MS.gene009635:intron 50.0%
CGAGCCATAGCAGCAGTAAC+AGG + chr5.3:60811884-60811903 None:intergenic 55.0%
TCCGAACCGTGTTTCTCGAG+AGG - chr5.3:60812391-60812410 MS.gene009635:intron 55.0%
!! CAACGTGGAATTGGTGCCAG+AGG - chr5.3:60811697-60811716 MS.gene009635:intron 55.0%
AGGGGGACCAACGACAACGA+TGG - chr5.3:60810523-60810542 MS.gene009635:CDS 60.0%
CTCGCTGCCATCGTTGTCGT+TGG + chr5.3:60810533-60810552 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 60809634 60812634 60809634 ID=MS.gene009635
chr5.3 mRNA 60809634 60812634 60809634 ID=MS.gene009635.t1;Parent=MS.gene009635
chr5.3 exon 60812626 60812634 60812626 ID=MS.gene009635.t1.exon1;Parent=MS.gene009635.t1
chr5.3 CDS 60812626 60812634 60812626 ID=cds.MS.gene009635.t1;Parent=MS.gene009635.t1
chr5.3 exon 60812480 60812546 60812480 ID=MS.gene009635.t1.exon2;Parent=MS.gene009635.t1
chr5.3 CDS 60812480 60812546 60812480 ID=cds.MS.gene009635.t1;Parent=MS.gene009635.t1
chr5.3 exon 60812253 60812383 60812253 ID=MS.gene009635.t1.exon3;Parent=MS.gene009635.t1
chr5.3 CDS 60812253 60812383 60812253 ID=cds.MS.gene009635.t1;Parent=MS.gene009635.t1
chr5.3 exon 60810343 60810837 60810343 ID=MS.gene009635.t1.exon4;Parent=MS.gene009635.t1
chr5.3 CDS 60810343 60810837 60810343 ID=cds.MS.gene009635.t1;Parent=MS.gene009635.t1
chr5.3 exon 60809634 60809882 60809634 ID=MS.gene009635.t1.exon5;Parent=MS.gene009635.t1
chr5.3 CDS 60809634 60809882 60809634 ID=cds.MS.gene009635.t1;Parent=MS.gene009635.t1
Gene Sequence

>MS.gene009635

ATGAAGAAGGTATGCTTATTATTAGTTATATACTTATAGATATTAAGTAGATTTCAGACTTTTTCTAACATGTTTTGTTTTTGTGCAGGTTTTATGTTTAAATATATGGATTTTCTTCAACAAAAGAAAAATGATCAAGTTCTCAAACAGAAAAGGTAATTATTTTTTATTCTTATTTGTGGTTTGTAATCTTAGATTAAGGCAATTTGTCTGAATTTCAACTAACCATTATTTACCTTTTTCAATCGCAGGTATTGCCAGAGAGTTTAATATTGAAAAGTCCACTTATAAGTTTAATAGTGCAGCATATTGTACTTTTAGTAAACCGGATTCATATTGTACTCTCTTTAAAAATGTAACTGACTCGAGTTGTAATATAGAAGTGAGTTTTGTAATGATCAATTATTATATTACAATATATTTATGTTATACATTATATTTTTAGTGAGTTTATTTATTTGACTTATATTCCTACTACAATTAATTATCAAAATAAATTATGATATCTTAGTTATTTATCAATTGTAGTTTACAAATAAATTGAAGTATAACACATATTACTTTAAATAAAAAAAGTTAACACATAAATGTGAATAATAATCTCAAAAGATCTATATTCCATAACTTGGTCAAATAAATATATATCAGAATGACTAAATATTATACTCAAAGAAGTTGGTATTTAAGTGGTATGTGTATATAGCAAATCAATGTTTGGACTTGAACAAACTTACATCTCAATTGAGCCCAATAAACCAAGCAAAAATGTGTGGCTGTTAAATACTCAAAATAAAATAATAACCTTGCATATACTTTCTACCAACGTTTTTCACCTGAAACACAATCACACTCATCCATTTCCTTCGCCATTGAAATCGCAAGCGTGCAAAGGGGGACCAACGACAACGATGGCAGCGAGAAACCAAGTGTACGACATCCTTTATACCCTTTTTCACTCATAATTTTGTTTTTTTCCCCTTCAATTTCGAAAATCCAATGCATGTCCCCTTGCAAATCGCAAGTCCTATATGCTTGCACATTCATTTCTGATTTTTTTTTTTGGAATTTCGCTTAATAGGCAAACTGTCTACTTCATTATTTAAGTGTTTATTTAAGTTTACATGTGCATAATTTTTGCAGTATATCCGATTTTGGAAAAAGGGTATATGCACGAATAGAGGTCTACTCGATTTCTTTCACTTTTATATATACTTAACTATAAAAGGAATAGAGGTCCATGCAAGATTAGAGGACATGCACGAATGATTTGTTGCAATCTCATCATTATTTGTACCTTAAAAAGTGTTTTAATTTTTGATATTATACCATTGTTGGTTATATTTTCCTGTAAGCTTTAAACTGAATGATTTGTTCTAATCACATCATGTTCTAATCACATCATGTTCTAAGCTTTAAACTAAATTTTTGATATTATACCATTGTTGGTTATATTTTCCTGTAAGCTTTAAACTGAATGATTTGTTCTAATCACATCGAACTATGTCCTTGAATTAATTGTAGACATTTGGAGGGCAGGGATTTTTTCCTAACTATGGTGAACCCGGAAGAGGTATGTACACACTATTTATATCGACTCATCATGTTTAATGTTTAGTGCAAGTGTTTCATTAGATTTGGGTTTACATAAACATAAAAACAATTTAGTATACATTGTTATACATGAGGGTTAAATTTCATTTTTTGTTTGGTGCGGCGATAGGTTTCTTCGCGTTTATTATTTATAAAATATGAAGTATATTAGAATTATTATTGAACCATTTATTTTGTTGCTTTTAGGATATTATACCCGTGAGTTTCGAGTTTTTCCTAAGAAATCAAGACACTCTGCAATTAACAAAACATGGATTTGTTAGGGATAATGAAGGTAGAGGGATTGTTATCCGTTTAGCCATGGATGACACTGGAGTTTCCATGAGGAACGGTGCCTTAATTGCTAAAAAGTTTGGCTTGCAAGTCCCCACTATCGCCAAATTCTATTATCTTGGTCCATTGAACATGTTCTTCGTTAGCTTCTTGCCTGATGCTAACATACAAGAAGCTAACAACGTGGAATTGGTGCCAGAGGCTTTACACAATCCACAAGGTGTGCAGGAAGTTATTCAGATGGAAAAGGTTGTTCAAGAAGTGATTGACATTAGTTCGGATGAAGAGGTACACATGAATGGAGATGAAGAACAGCAGGATGAAGTTGTGCAAGAAGAGGAAAATCTCCATCGTTTTGAGGTTCCTGTTACTGCTGCTATGGCTCGGTCTATTACGGGACAACCAATGGTAAGATATACAAAAAACAAACCTTCTATCTTCTTTATAATTCATTCGGAATATCAATTTATTTTATGAATAATATATATATATTGTACTACTCAAATGCAATATTCCACATTTTGTTATATGCGCTTCATATATATATAAATATTTAAATAAATTATCATGATTATCAATTTTCACATGTCCCTTTATGTTTGTTCATGATCCATAGGTAGGTGCATTTTTTAAGTTTGGTTTAATAACTATTGATATTGTAAATTAATTTTAGTTCAAATGTGGCTGTTAAAAACAAAACATAATTCAAAGTATATCTAATTGGCAAAATTTAGTAGTTTTAGAAGTTACTACAAGATGTCACATAGTGCCTTGGTTATTTATGTTTTATACATAAAATTAATGATGTACATTTGATTAAAATTGTATGGTGCAATTGACTAAGTTATTTTACTTTTATGTTGGTTAGCATTTTCCGAACCGTGTTTCTCGAGAGGTTTTGAAACAACCAAGGAAGTGGATGACGTTGGTTGACATGGAACTTGATAGAGAATATAGGTGCAAGCTAAACATCGCTGAACGGAACAAAGATGAGAAATCATTGGGAAGAGGATGGTGGAAGTTTGTTAAAGATCGCCGCCTAAAAGAGGGTGACAAGCTTATATTTGAGTTGGATCGCCCTCCTACAGTTCTCTTTGTGAAGGTTGTGCCTAGGTAG
Protein sequence

>MS.gene009635.t1

MKKVLCLNIWIFFNKRKMIKFSNRKGIAREFNIEKSTYKFNSAAYCTFSKPDSYCTLFKNVTDSSCNIEDIIPVSFEFFLRNQDTLQLTKHGFVRDNEGRGIVIRLAMDDTGVSMRNGALIAKKFGLQVPTIAKFYYLGPLNMFFVSFLPDANIQEANNVELVPEALHNPQGVQEVIQMEKVVQEVIDISSDEEVHMNGDEEQQDEVVQEEENLHRFEVPVTAAMARSITGQPMHFPNRVSREVLKQPRKWMTLVDMELDREYRCKLNIAERNKDEKSLGRGWWKFVKDRRLKEGDKLIFELDRPPTVLFVKVVPR