AlfalfaGEDB | MS.gene009668

M. sativa cultivar XinJiangDaYe / MS.gene009668

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009668.t1	XP_003616031.1	92	162	13	0	1	162	1	162	5.80E-79	303.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009668.t1	Q9FJK3	38.6	153	94	0	1	153	1	153	1.5e-27	124.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009668.t1	G7K0T4	92.0	162	13	0	1	162	1	162	4.2e-79	303.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene009668.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009668.t1	MTR_5g075380	91.975	162	13	0	1	162	1	162	5.13e-113	317
MS.gene009668.t1	MTR_3g466980	41.250	160	94	0	1	160	1	160	1.02e-44	146
MS.gene009668.t1	MTR_3g467080	43.506	154	86	1	1	153	1	154	2.07e-41	136
MS.gene009668.t1	MTR_4g032620	42.208	154	88	1	1	153	1	154	1.04e-39	134
MS.gene009668.t1	MTR_4g031910	46.667	150	79	1	1	149	1	150	8.84e-39	132
MS.gene009668.t1	MTR_3g065100	42.138	159	92	0	1	159	1	159	1.62e-38	131
MS.gene009668.t1	MTR_3g466830	42.857	154	88	0	4	157	3	156	3.62e-37	125
MS.gene009668.t1	MTR_3g466890	42.949	156	89	0	5	160	4	159	2.51e-36	123
MS.gene009668.t1	MTR_4g063790	38.125	160	92	2	1	156	1	157	5.66e-35	120
MS.gene009668.t1	MTR_2g016210	34.810	158	102	1	1	157	1	158	8.45e-35	122
MS.gene009668.t1	MTR_1g077320	40.385	156	92	1	1	156	1	155	7.16e-34	117
MS.gene009668.t1	MTR_8g036130	40.132	152	88	2	1	149	1	152	2.03e-33	118
MS.gene009668.t1	MTR_1g077300	40.385	156	90	2	1	156	1	153	5.55e-33	114
MS.gene009668.t1	MTR_1g077390	37.179	156	97	1	1	156	1	155	5.55e-33	114
MS.gene009668.t1	MTR_3g031100	36.364	154	97	1	1	153	1	154	1.16e-32	116
MS.gene009668.t1	MTR_5g047580	35.256	156	100	1	1	156	1	155	2.11e-31	110
MS.gene009668.t1	MTR_7g011950	39.103	156	94	1	1	156	1	155	3.46e-31	110
MS.gene009668.t1	MTR_4g032260	43.506	154	86	1	1	153	1	154	1.02e-30	109
MS.gene009668.t1	MTR_4g032290	44.667	150	82	1	1	149	1	150	1.16e-30	111
MS.gene009668.t1	MTR_4g028720	37.821	156	96	1	1	156	1	155	4.56e-30	107
MS.gene009668.t1	MTR_3g465410	39.394	132	73	2	29	156	1	129	5.37e-30	107
MS.gene009668.t1	MTR_1g084950	37.821	156	96	1	1	156	1	155	2.95e-29	105
MS.gene009668.t1	MTR_2g035610	44.628	121	67	0	1	121	1	121	1.02e-28	102
MS.gene009668.t1	MTR_1g090697	33.758	157	103	1	1	157	1	156	1.51e-28	103
MS.gene009668.t1	MTR_1g090783	33.117	154	102	1	4	157	3	155	5.58e-28	102
MS.gene009668.t1	MTR_3g031240	33.117	154	102	1	1	153	1	154	6.66e-28	102
MS.gene009668.t1	MTR_1g090710	34.810	158	101	2	1	157	1	157	1.22e-26	98.6
MS.gene009668.t1	MTR_2g035580	34.641	153	100	0	1	153	1	153	5.43e-26	97.1
MS.gene009668.t1	MTR_3g466900	37.405	131	82	0	32	162	2	132	2.14e-25	94.7
MS.gene009668.t1	MTR_3g466930	37.984	129	80	0	32	160	2	130	4.71e-25	94.0
MS.gene009668.t1	MTR_3g093900	31.613	155	106	0	4	158	7	161	5.67e-23	94.7
MS.gene009668.t1	MTR_4g028800	54.795	73	31	1	1	73	1	71	1.09e-22	85.9
MS.gene009668.t1	MTR_7g106510	33.121	157	103	2	4	159	7	162	1.76e-22	93.2
MS.gene009668.t1	MTR_4g019670	31.410	156	107	0	4	159	7	162	1.42e-21	90.5
MS.gene009668.t1	MTR_5g047560	30.128	156	92	2	1	156	1	139	1.87e-18	77.0
MS.gene009668.t1	MTR_1g114730	32.773	119	72	4	1	112	1	118	1.41e-14	70.1
MS.gene009668.t1	MTR_6g018920	32.231	121	74	4	1	114	1	120	6.29e-14	68.2
MS.gene009668.t1	MTR_7g055790	33.962	106	52	1	4	109	62	149	1.14e-11	59.7
MS.gene009668.t1	MTR_6g005450	31.858	113	70	3	1	107	1	112	1.68e-11	61.2
MS.gene009668.t1	MTR_7g055800	34.177	79	37	1	31	109	18	81	3.42e-11	57.0
MS.gene009668.t1	MTR_6g005440	27.711	166	105	5	1	160	1	157	7.05e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009668.t1	AT5G48670	39.610	154	91	2	1	153	1	153	8.44e-31	113
MS.gene009668.t1	AT3G05860	31.544	149	102	0	1	149	1	149	6.49e-27	100
MS.gene009668.t1	AT1G65300	35.971	139	89	0	4	142	3	141	8.17e-27	102
MS.gene009668.t1	AT1G65330	42.857	105	60	0	5	109	4	108	5.39e-26	100
MS.gene009668.t1	AT3G05860	31.544	149	102	0	1	149	1	149	5.78e-26	99.4
MS.gene009668.t1	AT3G05860	31.544	149	102	0	1	149	1	149	7.66e-26	99.4
MS.gene009668.t1	AT5G26650	44.340	106	59	0	4	109	2	107	3.76e-25	99.4
MS.gene009668.t1	AT5G26630	32.704	159	107	0	1	159	1	159	4.40e-25	96.3
MS.gene009668.t1	AT5G27960	43.396	106	60	0	4	109	2	107	1.21e-24	97.4
MS.gene009668.t1	AT1G31630	31.410	156	106	1	4	159	3	157	1.69e-24	97.4
MS.gene009668.t1	AT1G31640	38.462	117	67	2	5	121	4	115	3.35e-23	95.1
MS.gene009668.t1	AT5G27810	38.372	86	53	0	29	114	1	86	5.79e-20	80.5
MS.gene009668.t1	AT2G28700	34.862	109	71	0	1	109	1	109	9.49e-19	81.6
MS.gene009668.t1	AT2G40210	41.584	101	59	0	1	101	1	101	2.43e-18	80.9
MS.gene009668.t1	AT5G26580	38.532	109	53	1	1	109	1	95	1.55e-17	78.6
MS.gene009668.t1	AT5G06500	29.487	156	103	2	1	153	1	152	2.49e-16	73.9
MS.gene009668.t1	AT1G22590	30.435	115	80	0	1	115	1	115	8.46e-16	70.9
MS.gene009668.t1	AT2G15660	33.028	109	72	1	38	146	31	138	4.50e-12	62.4
MS.gene009668.t1	AT5G55690	41.463	82	41	2	1	75	1	82	1.17e-11	61.6
MS.gene009668.t1	AT5G55690	41.463	82	41	2	1	75	1	82	1.17e-11	61.6
MS.gene009668.t1	AT5G58890	37.273	110	62	2	1	104	1	109	1.92e-11	61.2

Find 41 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAACTTCTCATTCAACTTT+TGG	0.190821	5.3:+60188717	None:intergenic
ATGATCTTGAACCACAAATT+TGG	0.211992	5.3:-60188847	MS.gene009668:CDS
TGCTCTCCTTGCTGTGTGAT+TGG	0.345198	5.3:+60188964	None:intergenic
TCTCATGGTTGAACATCTTT+CGG	0.376961	5.3:+60188759	None:intergenic
CTTCACTTTCTTTCCTGCCA+TGG	0.385906	5.3:+60189000	None:intergenic
AGAAGAAAATGAAGGAGAAT+AGG	0.402400	5.3:-60188694	MS.gene009668:CDS
ATTTGTGGTTCAAGATCATC+AGG	0.402521	5.3:+60188851	None:intergenic
TGATATGAAGATGGTGCAAT+TGG	0.408742	5.3:-60188552	MS.gene009668:CDS
GGAGAGCAACGTACAAGAAA+AGG	0.412101	5.3:-60188949	MS.gene009668:CDS
TGAATCGAACCAGCACACTA+TGG	0.431382	5.3:+60188807	None:intergenic
TGAACGGATGGCCAAATTTG+TGG	0.436771	5.3:+60188836	None:intergenic
GGAGAAGGATGAGAGGGCTT+AGG	0.440373	5.3:-60188531	None:intergenic
TCTGAAGTGCTTAAATCCTT+AGG	0.453968	5.3:+60188620	None:intergenic
CAAATTTGGCCATCCGTTCA+AGG	0.457472	5.3:-60188833	MS.gene009668:CDS
CAACCATGAGAGTTACTTGA+AGG	0.466117	5.3:-60188747	MS.gene009668:CDS
TTTCTCAATACCACCATGGC+AGG	0.467282	5.3:-60189013	None:intergenic
GAGAAGGATGAGAGGGCTTA+GGG	0.469907	5.3:-60188530	None:intergenic
TTGCATCACTATCTTGAGGC+AGG	0.484072	5.3:-60188650	MS.gene009668:CDS
ATGTGATTCATGAGAAAATG+AGG	0.503498	5.3:-60188580	MS.gene009668:CDS
GAAGTTGAAGAAGAAAATGA+AGG	0.507311	5.3:-60188702	MS.gene009668:CDS
GAGAGCAACGTACAAGAAAA+GGG	0.528840	5.3:-60188948	MS.gene009668:CDS
TTTCCTTCAAGTAACTCTCA+TGG	0.530880	5.3:+60188744	None:intergenic
TGAGGCAGGGAATGTTCCTA+AGG	0.542520	5.3:-60188636	MS.gene009668:CDS
ACATGCTTGAACTCCACATA+GGG	0.545908	5.3:+60188889	None:intergenic
TGCAATTGGAGAAGGATGAG+AGG	0.552324	5.3:-60188538	MS.gene009668:CDS
ATGAGAGTTACTTGAAGGAA+AGG	0.555751	5.3:-60188742	MS.gene009668:CDS
CTATGGACTCCTTGAACGGA+TGG	0.558436	5.3:+60188824	None:intergenic
AATGAAATCACAACCCTATG+TGG	0.562718	5.3:-60188902	MS.gene009668:CDS
CACACTATGGACTCCTTGAA+CGG	0.564303	5.3:+60188820	None:intergenic
TCAAGGAGTCCATAGTGTGC+TGG	0.565483	5.3:-60188816	MS.gene009668:CDS
GAGGGAAATTGATATGAAGA+TGG	0.575041	5.3:-60188561	MS.gene009668:CDS
TGAAGGAGAATAGGATGATG+TGG	0.588946	5.3:-60188685	MS.gene009668:CDS
TGCATCACTATCTTGAGGCA+GGG	0.606928	5.3:-60188649	MS.gene009668:CDS
GAAGATGGTGCAATTGGAGA+AGG	0.611243	5.3:-60188546	MS.gene009668:CDS
GCAATTGGAGAAGGATGAGA+GGG	0.619379	5.3:-60188537	MS.gene009668:CDS
CACATGCTTGAACTCCACAT+AGG	0.630810	5.3:+60188888	None:intergenic
TCAATTGCATCACTATCTTG+AGG	0.632807	5.3:-60188654	MS.gene009668:CDS
TGTGATTCATGAGAAAATGA+GGG	0.634781	5.3:-60188579	MS.gene009668:CDS
TCATAACCAATCACACAGCA+AGG	0.650603	5.3:-60188970	MS.gene009668:CDS
CTATGTGGAGTTCAAGCATG+TGG	0.662987	5.3:-60188887	MS.gene009668:CDS
GGTTCGATTCATGCATACTG+CGG	0.703185	5.3:-60188795	MS.gene009668:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGAAGAAAATGAAGGAGAAT+AGG	-	chr5.3:60188836-60188855	MS.gene009668:CDS	30.0%
	ATGATCTTGAACCACAAATT+TGG	-	chr5.3:60188683-60188702	MS.gene009668:CDS	30.0%
	ATGTGATTCATGAGAAAATG+AGG	-	chr5.3:60188950-60188969	MS.gene009668:CDS	30.0%
	GAAGTTGAAGAAGAAAATGA+AGG	-	chr5.3:60188828-60188847	MS.gene009668:CDS	30.0%
	TGTGATTCATGAGAAAATGA+GGG	-	chr5.3:60188951-60188970	MS.gene009668:CDS	30.0%
!	TCAACTTCTCATTCAACTTT+TGG	+	chr5.3:60188816-60188835	None:intergenic	30.0%
	AATGAAATCACAACCCTATG+TGG	-	chr5.3:60188628-60188647	MS.gene009668:CDS	35.0%
	ATGAGAGTTACTTGAAGGAA+AGG	-	chr5.3:60188788-60188807	MS.gene009668:CDS	35.0%
	ATTTGTGGTTCAAGATCATC+AGG	+	chr5.3:60188682-60188701	None:intergenic	35.0%
	GAGGGAAATTGATATGAAGA+TGG	-	chr5.3:60188969-60188988	MS.gene009668:CDS	35.0%
	TCAATTGCATCACTATCTTG+AGG	-	chr5.3:60188876-60188895	MS.gene009668:CDS	35.0%
	TCTCATGGTTGAACATCTTT+CGG	+	chr5.3:60188774-60188793	None:intergenic	35.0%
	TGATATGAAGATGGTGCAAT+TGG	-	chr5.3:60188978-60188997	MS.gene009668:CDS	35.0%
	TTTCCTTCAAGTAACTCTCA+TGG	+	chr5.3:60188789-60188808	None:intergenic	35.0%
!	TCTGAAGTGCTTAAATCCTT+AGG	+	chr5.3:60188913-60188932	None:intergenic	35.0%
	ACATGCTTGAACTCCACATA+GGG	+	chr5.3:60188644-60188663	None:intergenic	40.0%
	CAACCATGAGAGTTACTTGA+AGG	-	chr5.3:60188783-60188802	MS.gene009668:CDS	40.0%
	GAGAGCAACGTACAAGAAAA+GGG	-	chr5.3:60188582-60188601	MS.gene009668:CDS	40.0%
	TCATAACCAATCACACAGCA+AGG	-	chr5.3:60188560-60188579	MS.gene009668:CDS	40.0%
	TGAAGGAGAATAGGATGATG+TGG	-	chr5.3:60188845-60188864	MS.gene009668:CDS	40.0%
	CAAATTTGGCCATCCGTTCA+AGG	-	chr5.3:60188697-60188716	MS.gene009668:CDS	45.0%
	CACACTATGGACTCCTTGAA+CGG	+	chr5.3:60188713-60188732	None:intergenic	45.0%
	CACATGCTTGAACTCCACAT+AGG	+	chr5.3:60188645-60188664	None:intergenic	45.0%
	CTATGTGGAGTTCAAGCATG+TGG	-	chr5.3:60188643-60188662	MS.gene009668:CDS	45.0%
	GAAGATGGTGCAATTGGAGA+AGG	-	chr5.3:60188984-60189003	MS.gene009668:CDS	45.0%
	GCAATTGGAGAAGGATGAGA+GGG	-	chr5.3:60188993-60189012	MS.gene009668:CDS	45.0%
	GGAGAGCAACGTACAAGAAA+AGG	-	chr5.3:60188581-60188600	MS.gene009668:CDS	45.0%
	GGTTCGATTCATGCATACTG+CGG	-	chr5.3:60188735-60188754	MS.gene009668:CDS	45.0%
	TGAACGGATGGCCAAATTTG+TGG	+	chr5.3:60188697-60188716	None:intergenic	45.0%
	TGAATCGAACCAGCACACTA+TGG	+	chr5.3:60188726-60188745	None:intergenic	45.0%
	TGCAATTGGAGAAGGATGAG+AGG	-	chr5.3:60188992-60189011	MS.gene009668:CDS	45.0%
	TGCATCACTATCTTGAGGCA+GGG	-	chr5.3:60188881-60188900	MS.gene009668:CDS	45.0%
	TTGCATCACTATCTTGAGGC+AGG	-	chr5.3:60188880-60188899	MS.gene009668:CDS	45.0%
	CTATGGACTCCTTGAACGGA+TGG	+	chr5.3:60188709-60188728	None:intergenic	50.0%
	TCAAGGAGTCCATAGTGTGC+TGG	-	chr5.3:60188714-60188733	MS.gene009668:CDS	50.0%
	TGAGGCAGGGAATGTTCCTA+AGG	-	chr5.3:60188894-60188913	MS.gene009668:CDS	50.0%
!	TGCTCTCCTTGCTGTGTGAT+TGG	+	chr5.3:60188569-60188588	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	60188532	60189020	60188532	ID=MS.gene009668
chr5.3	mRNA	60188532	60189020	60188532	ID=MS.gene009668.t1;Parent=MS.gene009668
chr5.3	exon	60188532	60189020	60188532	ID=MS.gene009668.t1.exon1;Parent=MS.gene009668.t1
chr5.3	CDS	60188532	60189020	60188532	ID=cds.MS.gene009668.t1;Parent=MS.gene009668.t1

Gene Sequence

>MS.gene009668

ATGGCAGGAAAGAAAGTGAAGCTTGCATTCATAACCAATCACACAGCAAGGAGAGCAACGTACAAGAAAAGGGTACAAAGTTTGATGAAGAAGCTCAATGAAATCACAACCCTATGTGGAGTTCAAGCATGTGGTATAGTTTTCAAGCCTGATGATCTTGAACCACAAATTTGGCCATCCGTTCAAGGAGTCCATAGTGTGCTGGTTCGATTCATGCATACTGCGGACTTCGATCGAGACCGAAAGATGTTCAACCATGAGAGTTACTTGAAGGAAAGGATCCAAAAGTTGAATGAGAAGTTGAAGAAGAAAATGAAGGAGAATAGGATGATGTGGATGAGTGTTCAATTGCATCACTATCTTGAGGCAGGGAATGTTCCTAAGGATTTAAGCACTTCAGATATGAATGATCTCACATATGTGATTCATGAGAAAATGAGGGAAATTGATATGAAGATGGTGCAATTGGAGAAGGATGAGAGGGCTTAG

Protein sequence

>MS.gene009668.t1

MAGKKVKLAFITNHTARRATYKKRVQSLMKKLNEITTLCGVQACGIVFKPDDLEPQIWPSVQGVHSVLVRFMHTADFDRDRKMFNHESYLKERIQKLNEKLKKKMKENRMMWMSVQLHHYLEAGNVPKDLSTSDMNDLTYVIHEKMREIDMKMVQLEKDERA