AlfalfaGEDB | MS.gene009751

M. sativa cultivar XinJiangDaYe / MS.gene009751

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009751.t1	AES98839.2	88.2	85	10	0	1	85	1	85	9.40E-36	159.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009751.t1	Q9LFQ3	46.2	65	33	2	10	74	51	113	1.3e-06	53.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009751.t1	G7JZR3	88.2	85	10	0	1	85	1	85	6.7e-36	159.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene009751.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene009751.t1	MTR_5g073430	87.209	86	11	0	1	86	1	86	3.37e-52	159
MS.gene009751.t1	MTR_7g083950	63.291	79	29	0	1	79	2	80	1.86e-28	105
MS.gene009751.t1	MTR_3g006150	49.153	59	30	0	16	74	94	152	3.35e-11	56.2
MS.gene009751.t1	MTR_8g062700	47.059	68	32	3	12	79	43	106	4.94e-11	57.4
MS.gene009751.t1	MTR_8g062700	47.059	68	32	3	12	79	43	106	5.03e-11	57.4
MS.gene009751.t1	MTR_1g105960	47.059	68	32	3	12	79	2	65	9.44e-11	53.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCATCAGGGTTTATTATT+CGG	0.287310	5.3:+58292110	MS.gene009751:CDS
TGCACTGGAGTATCTTCTTA+AGG	0.379025	5.3:+58291959	MS.gene009751:CDS
TGGGATGCTTCAAAGGCATC+AGG	0.403391	5.3:+58292097	MS.gene009751:CDS
GATACTCCAGTGCATGTTCA+TGG	0.422585	5.3:-58291950	None:intergenic
GCATCCCACTAACTCTTGAT+TGG	0.437559	5.3:-58292082	None:intergenic
GCACTGGAGTATCTTCTTAA+GGG	0.471379	5.3:+58291960	MS.gene009751:CDS
AAAACTCACAAGAGATTACA+AGG	0.479131	5.3:+58292034	MS.gene009751:CDS
ACCTTCCTCATCATGCCAGC+TGG	0.479240	5.3:+58292140	MS.gene009751:CDS
CTCACAAGAGATTACAAGGC+TGG	0.498781	5.3:+58292038	MS.gene009751:CDS
GCTGGAAGAATTGATATAAG+AGG	0.503169	5.3:+58292056	MS.gene009751:CDS
GCTGGTTTGAACTCTTTCCA+TGG	0.521060	5.3:+58292158	MS.gene009751:CDS
GAGTTAGTGGGATGCTTCAA+AGG	0.526784	5.3:+58292090	MS.gene009751:CDS
GGCATCCAATCAAGAGTTAG+TGG	0.541734	5.3:+58292077	MS.gene009751:CDS
TTATTGCCATGAACATGCAC+TGG	0.547671	5.3:+58291944	MS.gene009751:CDS
TGGAAAGAGTTCAAACCAGC+TGG	0.553560	5.3:-58292155	None:intergenic
TGTTCATGGCAATAATTTCG+AGG	0.557832	5.3:-58291936	None:intergenic
GGGATGCTTCAAAGGCATCA+GGG	0.590682	5.3:+58292098	MS.gene009751:CDS
TCAAACCAGCTGGCATGATG+AGG	0.594872	5.3:-58292145	None:intergenic
ATTATGTGAATTACGTACCA+TGG	0.602512	5.3:-58292175	None:intergenic
GTTCATGGCAATAATTTCGA+GGG	0.608298	5.3:-58291935	None:intergenic
ACCAGCTGGCATGATGAGGA+AGG	0.612834	5.3:-58292141	None:intergenic
AGGGAGTGAAACTTGCATCT+CGG	0.621205	5.3:+58291979	MS.gene009751:CDS
GCATCCAATCAAGAGTTAGT+GGG	0.632249	5.3:+58292078	MS.gene009751:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAACTCACAAGAGATTACA+AGG	+	chr5.3:58292034-58292053	MS.gene009751:CDS	30.0%
	AGGCATCAGGGTTTATTATT+CGG	+	chr5.3:58292110-58292129	MS.gene009751:CDS	35.0%
	GCTGGAAGAATTGATATAAG+AGG	+	chr5.3:58292056-58292075	MS.gene009751:CDS	35.0%
	GTTCATGGCAATAATTTCGA+GGG	-	chr5.3:58291938-58291957	None:intergenic	35.0%
	TGTTCATGGCAATAATTTCG+AGG	-	chr5.3:58291939-58291958	None:intergenic	35.0%
	GCATCCAATCAAGAGTTAGT+GGG	+	chr5.3:58292078-58292097	MS.gene009751:CDS	40.0%
	TTATTGCCATGAACATGCAC+TGG	+	chr5.3:58291944-58291963	MS.gene009751:CDS	40.0%
!	GCACTGGAGTATCTTCTTAA+GGG	+	chr5.3:58291960-58291979	MS.gene009751:CDS	40.0%
!	TGCACTGGAGTATCTTCTTA+AGG	+	chr5.3:58291959-58291978	MS.gene009751:CDS	40.0%
	AGGGAGTGAAACTTGCATCT+CGG	+	chr5.3:58291979-58291998	MS.gene009751:CDS	45.0%
	CTCACAAGAGATTACAAGGC+TGG	+	chr5.3:58292038-58292057	MS.gene009751:CDS	45.0%
	GAGTTAGTGGGATGCTTCAA+AGG	+	chr5.3:58292090-58292109	MS.gene009751:CDS	45.0%
	GATACTCCAGTGCATGTTCA+TGG	-	chr5.3:58291953-58291972	None:intergenic	45.0%
	GCATCCCACTAACTCTTGAT+TGG	-	chr5.3:58292085-58292104	None:intergenic	45.0%
	GGCATCCAATCAAGAGTTAG+TGG	+	chr5.3:58292077-58292096	MS.gene009751:CDS	45.0%
	TGGAAAGAGTTCAAACCAGC+TGG	-	chr5.3:58292158-58292177	None:intergenic	45.0%
!!	GCTGGTTTGAACTCTTTCCA+TGG	+	chr5.3:58292158-58292177	MS.gene009751:CDS	45.0%
	TCAAACCAGCTGGCATGATG+AGG	-	chr5.3:58292148-58292167	None:intergenic	50.0%
!!	GGGATGCTTCAAAGGCATCA+GGG	+	chr5.3:58292098-58292117	MS.gene009751:CDS	50.0%
!!	TGGGATGCTTCAAAGGCATC+AGG	+	chr5.3:58292097-58292116	MS.gene009751:CDS	50.0%
	ACCAGCTGGCATGATGAGGA+AGG	-	chr5.3:58292144-58292163	None:intergenic	55.0%
	ACCTTCCTCATCATGCCAGC+TGG	+	chr5.3:58292140-58292159	MS.gene009751:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	58291927	58292187	58291927	ID=MS.gene009751
chr5.3	mRNA	58291927	58292187	58291927	ID=MS.gene009751.t1;Parent=MS.gene009751
chr5.3	exon	58291927	58292187	58291927	ID=MS.gene009751.t1.exon1;Parent=MS.gene009751.t1
chr5.3	CDS	58291927	58292187	58291927	ID=cds.MS.gene009751.t1;Parent=MS.gene009751.t1

Gene Sequence	>MS.gene009751 ATGAACATCCCTCGAAATTATTGCCATGAACATGCACTGGAGTATCTTCTTAAGGGAGTGAAACTTGCATCTCGGTACGATAACAGCATAAAGTACGATGAGTTTTTAAAACTCACAAGAGATTACAAGGCTGGAAGAATTGATATAAGAGGCATCCAATCAAGAGTTAGTGGGATGCTTCAAAGGCATCAGGGTTTATTATTCGGATTCAACACCTTCCTCATCATGCCAGCTGGTTTGAACTCTTTCCATGGTACGTAA
Protein sequence	>MS.gene009751.t1 MNIPRNYCHEHALEYLLKGVKLASRYDNSIKYDEFLKLTRDYKAGRIDIRGIQSRVSGMLQRHQGLLFGFNTFLIMPAGLNSFHGT