Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009791.t1 | XP_003615824.1 | 95.4 | 217 | 9 | 1 | 1 | 216 | 1 | 217 | 6.90E-104 | 386.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009791.t1 | Q9LU68 | 53.8 | 91 | 40 | 2 | 125 | 215 | 337 | 425 | 2.3e-15 | 84.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009791.t1 | B7FH76 | 95.4 | 217 | 9 | 1 | 1 | 216 | 1 | 217 | 5.0e-104 | 386.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene009791.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009791.t1 | MTR_5g072780 | 96.774 | 217 | 6 | 1 | 1 | 216 | 1 | 217 | 2.62e-153 | 424 |
| MS.gene009791.t1 | MTR_7g032240 | 39.850 | 133 | 41 | 3 | 122 | 215 | 219 | 351 | 2.57e-20 | 87.8 |
| MS.gene009791.t1 | MTR_1g110870 | 44.144 | 111 | 42 | 3 | 123 | 215 | 316 | 424 | 1.44e-19 | 86.3 |
| MS.gene009791.t1 | MTR_4g127420 | 53.488 | 86 | 36 | 2 | 128 | 209 | 293 | 378 | 3.03e-19 | 85.1 |
| MS.gene009791.t1 | MTR_8g104190 | 41.727 | 139 | 53 | 4 | 93 | 215 | 274 | 400 | 3.22e-18 | 82.4 |
| MS.gene009791.t1 | MTR_1g013450 | 36.441 | 118 | 62 | 3 | 105 | 215 | 186 | 297 | 1.99e-13 | 68.2 |
| MS.gene009791.t1 | MTR_3g105710 | 67.442 | 43 | 14 | 0 | 173 | 215 | 232 | 274 | 1.92e-11 | 62.4 |
| MS.gene009791.t1 | MTR_4g128930 | 67.442 | 43 | 14 | 0 | 173 | 215 | 298 | 340 | 4.10e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009791.t1 | AT1G07050 | 67.000 | 100 | 26 | 3 | 118 | 215 | 99 | 193 | 3.16e-37 | 129 |
| MS.gene009791.t1 | AT1G07050 | 64.894 | 94 | 26 | 3 | 118 | 209 | 99 | 187 | 2.94e-32 | 116 |
| MS.gene009791.t1 | AT5G14370 | 53.211 | 109 | 36 | 3 | 122 | 215 | 228 | 336 | 2.78e-28 | 109 |
| MS.gene009791.t1 | AT4G25990 | 53.922 | 102 | 39 | 3 | 118 | 215 | 286 | 383 | 1.26e-23 | 97.4 |
| MS.gene009791.t1 | AT5G57180 | 53.846 | 91 | 40 | 2 | 125 | 215 | 337 | 425 | 6.33e-23 | 95.9 |
| MS.gene009791.t1 | AT1G73870 | 42.553 | 141 | 57 | 5 | 93 | 215 | 253 | 387 | 4.97e-21 | 90.1 |
| MS.gene009791.t1 | AT4G25990 | 47.009 | 117 | 39 | 4 | 118 | 215 | 286 | 398 | 7.21e-20 | 87.0 |
| MS.gene009791.t1 | AT4G25990 | 52.083 | 96 | 38 | 3 | 118 | 209 | 286 | 377 | 1.69e-19 | 85.9 |
| MS.gene009791.t1 | AT1G25440 | 39.869 | 153 | 73 | 5 | 82 | 215 | 251 | 403 | 1.93e-19 | 85.9 |
| MS.gene009791.t1 | AT1G49130 | 52.747 | 91 | 34 | 3 | 127 | 215 | 237 | 320 | 2.12e-19 | 85.1 |
| MS.gene009791.t1 | AT1G49130 | 52.747 | 91 | 34 | 3 | 127 | 215 | 230 | 313 | 2.22e-19 | 85.1 |
| MS.gene009791.t1 | AT5G57180 | 51.765 | 85 | 39 | 2 | 125 | 209 | 337 | 419 | 9.63e-19 | 84.0 |
| MS.gene009791.t1 | AT1G68520 | 41.525 | 118 | 51 | 3 | 116 | 215 | 282 | 399 | 1.02e-18 | 84.0 |
| MS.gene009791.t1 | AT2G24790 | 61.111 | 54 | 21 | 0 | 162 | 215 | 218 | 271 | 3.62e-13 | 67.4 |
| MS.gene009791.t1 | AT5G24930 | 53.226 | 62 | 29 | 0 | 154 | 215 | 276 | 337 | 5.23e-12 | 64.3 |
| MS.gene009791.t1 | AT5G57660 | 69.767 | 43 | 13 | 0 | 173 | 215 | 285 | 327 | 1.23e-11 | 63.5 |
Find 34 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCATGTTCTTCTTTCTT+TGG | 0.183818 | 5.3:-57375677 | None:intergenic |
| GGTCTGACCGTGGCTCTCTT+TGG | 0.244079 | 5.3:+57376056 | MS.gene009791:CDS |
| GCTCTCTCTTCTTCCAATAT+TGG | 0.250193 | 5.3:-57376858 | None:intergenic |
| TTTGAATTATCAAGAGGTTT+TGG | 0.259887 | 5.3:+57376027 | MS.gene009791:CDS |
| AAAAGTGTGACCTTGATTCT+TGG | 0.281388 | 5.3:-57376984 | None:intergenic |
| GAAGAGAAGCAAGTGTTCTT+AGG | 0.336563 | 5.3:+57376883 | MS.gene009791:CDS |
| GTCTGACCGTGGCTCTCTTT+GGG | 0.337400 | 5.3:+57376057 | MS.gene009791:CDS |
| GATGGGAGAAGTGCCAATAT+TGG | 0.355037 | 5.3:+57376845 | MS.gene009791:intron |
| AGTGTAAGATTTGTTGAAGT+TGG | 0.374346 | 5.3:-57375726 | None:intergenic |
| GGTATTTGAGTGGTAGCTTC+TGG | 0.391867 | 5.3:-57375701 | None:intergenic |
| ATAGTAGTAATGAGCTTCAT+TGG | 0.401933 | 5.3:+57375849 | MS.gene009791:CDS |
| CTCATCTTTCCCTTCATCAA+AGG | 0.415253 | 5.3:-57375897 | None:intergenic |
| TCATCTTTCCCTTCATCAAA+GGG | 0.428053 | 5.3:-57375896 | None:intergenic |
| AAGAGAGAAAATTACAATGA+TGG | 0.429279 | 5.3:+57375959 | MS.gene009791:CDS |
| CATAAGCAAATGCAATTATG+AGG | 0.471927 | 5.3:+57375922 | MS.gene009791:CDS |
| TGAGCTTCATTGGGATATCA+TGG | 0.472007 | 5.3:+57375859 | MS.gene009791:CDS |
| GAGGGATTTCCCTTTGATGA+AGG | 0.484035 | 5.3:+57375887 | MS.gene009791:CDS |
| ACTAACATAGGAGCCACTAT+TGG | 0.497136 | 5.3:-57376110 | None:intergenic |
| TAGTAGTAATGAGCTTCATT+GGG | 0.508412 | 5.3:+57375850 | MS.gene009791:CDS |
| AGGGATTTCCCTTTGATGAA+GGG | 0.520507 | 5.3:+57375888 | MS.gene009791:CDS |
| TCATTGGGATATCATGGAAT+GGG | 0.534580 | 5.3:+57375865 | MS.gene009791:CDS |
| TTCATTGGGATATCATGGAA+TGG | 0.539426 | 5.3:+57375864 | MS.gene009791:CDS |
| TTTGAATTTGAATTATCAAG+AGG | 0.541289 | 5.3:+57376021 | MS.gene009791:CDS |
| AACATAGGAGCCACTATTGG+AGG | 0.541652 | 5.3:-57376107 | None:intergenic |
| CGCTTTGTGAAGAGACATTG+AGG | 0.551371 | 5.3:+57377113 | MS.gene009791:CDS |
| TAAGATTTGTTGAAGTTGGA+TGG | 0.551571 | 5.3:-57375722 | None:intergenic |
| TGATAAAAGACCAAGAATCA+AGG | 0.578251 | 5.3:+57376974 | MS.gene009791:CDS |
| ATAGGAGCCACTATTGGAGG+AGG | 0.606351 | 5.3:-57376104 | None:intergenic |
| TTGGGATATCATGGAATGGG+AGG | 0.609318 | 5.3:+57375868 | MS.gene009791:CDS |
| GAAGTTGGATGGTATTTGAG+TGG | 0.615620 | 5.3:-57375711 | None:intergenic |
| TGGGATATCATGGAATGGGA+GGG | 0.659236 | 5.3:+57375869 | MS.gene009791:CDS |
| CATTAGCCCAAAGAGAGCCA+CGG | 0.677020 | 5.3:-57376063 | None:intergenic |
| AGTGGATGATGAAAAGAGTG+TGG | 0.691548 | 5.3:+57375991 | MS.gene009791:CDS |
| TTGGATGCATGGTCTGACCG+TGG | 0.694015 | 5.3:+57376046 | MS.gene009791:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAATATAGTTGTTATA+TGG | - | chr5.3:57376264-57376283 | None:intergenic | 10.0% |
| !!! | AATTTATTTTTTGTTTAGAT+GGG | + | chr5.3:57376828-57376847 | MS.gene009791:intron | 10.0% |
| !!! | TAATTTATTTTTTGTTTAGA+TGG | + | chr5.3:57376827-57376846 | MS.gene009791:intron | 10.0% |
| !!! | TAATTTTAAATAGTAACAAA+AGG | - | chr5.3:57376301-57376320 | None:intergenic | 10.0% |
| !!! | GTTTCTTTTTTTTTTTCATT+GGG | + | chr5.3:57377028-57377047 | MS.gene009791:intron | 15.0% |
| !!! | TATCAATTCTTATTCTTTTA+AGG | + | chr5.3:57376213-57376232 | MS.gene009791:intron | 15.0% |
| !!! | TGTTTCTTTTTTTTTTTCAT+TGG | + | chr5.3:57377027-57377046 | MS.gene009791:intron | 15.0% |
| !! | TTATATATGAAAGTGAAGAT+TGG | - | chr5.3:57376145-57376164 | None:intergenic | 20.0% |
| !! | TTTGAATTTGAATTATCAAG+AGG | + | chr5.3:57376021-57376040 | MS.gene009791:CDS | 20.0% |
| !!! | AAATTACAATGATGGTTTTT+TGG | + | chr5.3:57375967-57375986 | MS.gene009791:CDS | 20.0% |
| !!! | ATAACTAAATTTTTGGTGAT+AGG | + | chr5.3:57376576-57376595 | MS.gene009791:intron | 20.0% |
| !!! | GTAACATATTTAGTTCATTT+TGG | + | chr5.3:57376672-57376691 | MS.gene009791:intron | 20.0% |
| !!! | TTGTGATATTTTTTGATGTA+GGG | + | chr5.3:57377089-57377108 | MS.gene009791:intron | 20.0% |
| !!! | TTTGTCGATAACTAAATTTT+TGG | + | chr5.3:57376569-57376588 | MS.gene009791:intron | 20.0% |
| !!! | TTTGTGATATTTTTTGATGT+AGG | + | chr5.3:57377088-57377107 | MS.gene009791:intron | 20.0% |
| !!! | TTTTTGGATAAGTTGTTATA+TGG | - | chr5.3:57376239-57376258 | None:intergenic | 20.0% |
| ! | AAGAGAGAAAATTACAATGA+TGG | + | chr5.3:57375959-57375978 | MS.gene009791:CDS | 25.0% |
| ! | TATTACTTAATTGACACCAA+CGG | - | chr5.3:57376439-57376458 | None:intergenic | 25.0% |
| ! | TGGATAATGAATACTAACAT+AGG | - | chr5.3:57376125-57376144 | None:intergenic | 25.0% |
| !! | TAGTTGTTATATGGTCTTTT+TGG | - | chr5.3:57376255-57376274 | None:intergenic | 25.0% |
| !! | TTTGAATTATCAAGAGGTTT+TGG | + | chr5.3:57376027-57376046 | MS.gene009791:CDS | 25.0% |
| !!! | TATGTTGACTTTTGTTTAGA+TGG | + | chr5.3:57376457-57376476 | MS.gene009791:intron | 25.0% |
| ACTTCATGTTCTTCTTTCTT+TGG | - | chr5.3:57375680-57375699 | None:intergenic | 30.0% | |
| AGAAGAAGAAGAAGAACAAA+AGG | + | chr5.3:57375802-57375821 | MS.gene009791:CDS | 30.0% | |
| ATAGTAGTAATGAGCTTCAT+TGG | + | chr5.3:57375849-57375868 | MS.gene009791:CDS | 30.0% | |
| ATTACTTAATTGACACCAAC+GGG | - | chr5.3:57376438-57376457 | None:intergenic | 30.0% | |
| CACAATTGAATAGATCGAAT+AGG | - | chr5.3:57376542-57376561 | None:intergenic | 30.0% | |
| CATAAGCAAATGCAATTATG+AGG | + | chr5.3:57375922-57375941 | MS.gene009791:CDS | 30.0% | |
| CTATTCGATCTATTCAATTG+TGG | + | chr5.3:57376540-57376559 | MS.gene009791:intron | 30.0% | |
| TAGTAGTAATGAGCTTCATT+GGG | + | chr5.3:57375850-57375869 | MS.gene009791:CDS | 30.0% | |
| TGATAAAAGACCAAGAATCA+AGG | + | chr5.3:57376974-57376993 | MS.gene009791:CDS | 30.0% | |
| ! | CTACTATTACAAGTGTTTTG+TGG | - | chr5.3:57375836-57375855 | None:intergenic | 30.0% |
| !! | AGTGTAAGATTTGTTGAAGT+TGG | - | chr5.3:57375729-57375748 | None:intergenic | 30.0% |
| !! | TAAGATTTGTTGAAGTTGGA+TGG | - | chr5.3:57375725-57375744 | None:intergenic | 30.0% |
| GATAGGTAATCTTTGATGCT+TGG | + | chr5.3:57376593-57376612 | MS.gene009791:intron | 35.0% | |
| TCATCTTTCCCTTCATCAAA+GGG | - | chr5.3:57375899-57375918 | None:intergenic | 35.0% | |
| TCATTGGGATATCATGGAAT+GGG | + | chr5.3:57375865-57375884 | MS.gene009791:CDS | 35.0% | |
| TTCATTGGGATATCATGGAA+TGG | + | chr5.3:57375864-57375883 | MS.gene009791:CDS | 35.0% | |
| TTGATCGCTTTCTGGATAAT+AGG | + | chr5.3:57376494-57376513 | MS.gene009791:intron | 35.0% | |
| ! | AAAAGTGTGACCTTGATTCT+TGG | - | chr5.3:57376987-57377006 | None:intergenic | 35.0% |
| ! | GTCATAAAGATTTTACCCGT+TGG | + | chr5.3:57376420-57376439 | MS.gene009791:intron | 35.0% |
| !! | CAGCGTTTAATTTTCGAACT+TGG | - | chr5.3:57376956-57376975 | None:intergenic | 35.0% |
| !!! | CAATGATGGTTTTTTGGAAG+TGG | + | chr5.3:57375973-57375992 | MS.gene009791:CDS | 35.0% |
| AACTCTTGTTGATCGCTTTC+TGG | + | chr5.3:57376486-57376505 | MS.gene009791:intron | 40.0% | |
| ACTAACATAGGAGCCACTAT+TGG | - | chr5.3:57376113-57376132 | None:intergenic | 40.0% | |
| AGGGATTTCCCTTTGATGAA+GGG | + | chr5.3:57375888-57375907 | MS.gene009791:CDS | 40.0% | |
| AGTGGATGATGAAAAGAGTG+TGG | + | chr5.3:57375991-57376010 | MS.gene009791:CDS | 40.0% | |
| CTCATCTTTCCCTTCATCAA+AGG | - | chr5.3:57375900-57375919 | None:intergenic | 40.0% | |
| GAAGAGAAGCAAGTGTTCTT+AGG | + | chr5.3:57376883-57376902 | MS.gene009791:CDS | 40.0% | |
| GAAGTTGGATGGTATTTGAG+TGG | - | chr5.3:57375714-57375733 | None:intergenic | 40.0% | |
| GCTCTCTCTTCTTCCAATAT+TGG | - | chr5.3:57376861-57376880 | None:intergenic | 40.0% | |
| TGAGCTTCATTGGGATATCA+TGG | + | chr5.3:57375859-57375878 | MS.gene009791:CDS | 40.0% | |
| ! | TCATTTTCCTCCTCCAATAG+TGG | + | chr5.3:57376097-57376116 | MS.gene009791:CDS | 40.0% |
| !!! | ATCAAGAGGTTTTGGATGCA+TGG | + | chr5.3:57376035-57376054 | MS.gene009791:CDS | 40.0% |
| AACATAGGAGCCACTATTGG+AGG | - | chr5.3:57376110-57376129 | None:intergenic | 45.0% | |
| GAGGGATTTCCCTTTGATGA+AGG | + | chr5.3:57375887-57375906 | MS.gene009791:CDS | 45.0% | |
| GGTATTTGAGTGGTAGCTTC+TGG | - | chr5.3:57375704-57375723 | None:intergenic | 45.0% | |
| TGGGATATCATGGAATGGGA+GGG | + | chr5.3:57375869-57375888 | MS.gene009791:CDS | 45.0% | |
| TTGGGATATCATGGAATGGG+AGG | + | chr5.3:57375868-57375887 | MS.gene009791:CDS | 45.0% | |
| !! | GATGGGAGAAGTGCCAATAT+TGG | + | chr5.3:57376845-57376864 | MS.gene009791:intron | 45.0% |
| !! | TAAACAAAAAATAAATTATA+AGG | - | chr5.3:57376825-57376844 | None:intergenic | 5.0% |
| ATAGGAGCCACTATTGGAGG+AGG | - | chr5.3:57376107-57376126 | None:intergenic | 50.0% | |
| CATTAGCCCAAAGAGAGCCA+CGG | - | chr5.3:57376066-57376085 | None:intergenic | 50.0% | |
| GTCTGACCGTGGCTCTCTTT+GGG | + | chr5.3:57376057-57376076 | MS.gene009791:CDS | 55.0% | |
| TTGGATGCATGGTCTGACCG+TGG | + | chr5.3:57376046-57376065 | MS.gene009791:CDS | 55.0% | |
| GGTCTGACCGTGGCTCTCTT+TGG | + | chr5.3:57376056-57376075 | MS.gene009791:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 57375650 | 57377133 | 57375650 | ID=MS.gene009791 |
| chr5.3 | mRNA | 57375650 | 57377133 | 57375650 | ID=MS.gene009791.t1;Parent=MS.gene009791 |
| chr5.3 | exon | 57375650 | 57376126 | 57375650 | ID=MS.gene009791.t1.exon1;Parent=MS.gene009791.t1 |
| chr5.3 | CDS | 57375650 | 57376126 | 57375650 | ID=cds.MS.gene009791.t1;Parent=MS.gene009791.t1 |
| chr5.3 | exon | 57376846 | 57376995 | 57376846 | ID=MS.gene009791.t1.exon2;Parent=MS.gene009791.t1 |
| chr5.3 | CDS | 57376846 | 57376995 | 57376846 | ID=cds.MS.gene009791.t1;Parent=MS.gene009791.t1 |
| chr5.3 | exon | 57377110 | 57377133 | 57377110 | ID=MS.gene009791.t1.exon3;Parent=MS.gene009791.t1 |
| chr5.3 | CDS | 57377110 | 57377133 | 57377110 | ID=cds.MS.gene009791.t1;Parent=MS.gene009791.t1 |
| Gene Sequence |
| Protein sequence |
