Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009917.t1 | TDH72716.1 | 33.1 | 139 | 76 | 4 | 4 | 131 | 2 | 134 | 8.40E-08 | 67 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009917.t1 | Q9FKI4 | 42.3 | 97 | 50 | 3 | 35 | 131 | 44 | 134 | 6.5e-09 | 62.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009917.t1 | A0A484ECY5 | 33.1 | 139 | 76 | 4 | 4 | 131 | 2 | 134 | 6.0e-08 | 67.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene009917.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009917.t1 | MTR_3g034200 | 35.252 | 139 | 73 | 4 | 4 | 131 | 2 | 134 | 3.78e-13 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009917.t1 | AT5G13780 | 36.296 | 135 | 69 | 4 | 4 | 127 | 2 | 130 | 1.72e-12 | 62.4 |
Find 31 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCGTAATCCAAGGCCTTT+TGG | 0.266555 | 5.3:+55487584 | None:intergenic |
| AAACCTGTTCCAAATCCTTA+TGG | 0.271438 | 5.3:+55487915 | None:intergenic |
| CTTATCTTGCCTTTGAGTAC+TGG | 0.344775 | 5.3:-55488121 | MS.gene009917:CDS |
| GACTGCCGCCCATAAGGATT+TGG | 0.362443 | 5.3:-55487924 | MS.gene009917:CDS |
| CGCCCATAAGGATTTGGAAC+AGG | 0.390119 | 5.3:-55487918 | MS.gene009917:intron |
| GAAGGAGGAGGAGGTTAAGA+AGG | 0.410903 | 5.3:-55488026 | MS.gene009917:CDS |
| AACCTGTTCCAAATCCTTAT+GGG | 0.426781 | 5.3:+55487916 | None:intergenic |
| TTTATAAGCCGATATCTTCT+CGG | 0.436779 | 5.3:-55487525 | MS.gene009917:CDS |
| GCTTGTGACTGCCGCCCATA+AGG | 0.446228 | 5.3:-55487930 | MS.gene009917:CDS |
| TTATAAGCCGATATCTTCTC+GGG | 0.458239 | 5.3:-55487524 | MS.gene009917:CDS |
| TTGGTCTCCCGAGAAGATAT+CGG | 0.486784 | 5.3:+55487517 | None:intergenic |
| CTCTATTAAGAAGGAGGAGG+AGG | 0.487768 | 5.3:-55488035 | MS.gene009917:CDS |
| GGAGGAGGAGGTTAAGAAGG+AGG | 0.522170 | 5.3:-55488023 | MS.gene009917:CDS |
| AAGCTGGAATGTTGTACCTC+GGG | 0.522913 | 5.3:+55487617 | None:intergenic |
| CTCGCAACCTATCAGAGACT+TGG | 0.530721 | 5.3:-55487965 | MS.gene009917:CDS |
| TTATGTCTGCTCTATTAAGA+AGG | 0.559468 | 5.3:-55488044 | MS.gene009917:CDS |
| AGCGATACCAAGTCTCTGAT+AGG | 0.565478 | 5.3:+55487958 | None:intergenic |
| TTCATAAATATCGTAATCCA+AGG | 0.569142 | 5.3:+55487576 | None:intergenic |
| GCAAGATAAGATCATATTCG+CGG | 0.579984 | 5.3:+55488134 | None:intergenic |
| TTTCACGTCGCGGTCAGGAA+CGG | 0.583681 | 5.3:-55488088 | MS.gene009917:CDS |
| CTGCTCTATTAAGAAGGAGG+AGG | 0.589794 | 5.3:-55488038 | MS.gene009917:CDS |
| CACGTCGCGGTCAGGAACGG+CGG | 0.594718 | 5.3:-55488085 | MS.gene009917:CDS |
| CGCTGAAATCAGTCACGTCG+TGG | 0.599192 | 5.3:-55487990 | MS.gene009917:CDS |
| TGTTCATCTCCAGTACTCAA+AGG | 0.610835 | 5.3:+55488112 | None:intergenic |
| AAAGCTGGAATGTTGTACCT+CGG | 0.618480 | 5.3:+55487616 | None:intergenic |
| ACATCTCAGCGCTCGTCCCG+AGG | 0.619758 | 5.3:-55487633 | MS.gene009917:CDS |
| GGCGGCCGTCGCAATATCGT+CGG | 0.625495 | 5.3:-55488067 | MS.gene009917:CDS |
| ACGAGCGCTGAGATGTACTC+AGG | 0.636447 | 5.3:+55487640 | None:intergenic |
| TGTCTGCTCTATTAAGAAGG+AGG | 0.648646 | 5.3:-55488041 | MS.gene009917:CDS |
| CTGTTCCAAATCCTTATGGG+CGG | 0.673914 | 5.3:+55487919 | None:intergenic |
| CATAACCGACGATATTGCGA+CGG | 0.695901 | 5.3:+55488062 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACATATAGAATTACAGAACT+AGG | + | chr5.3:55487897-55487916 | None:intergenic | 25.0% |
| ! | CATATAGAATTACAGAACTA+GGG | + | chr5.3:55487896-55487915 | None:intergenic | 25.0% |
| ! | GTTCTGTAATTCTATATGTT+AGG | - | chr5.3:55487898-55487917 | MS.gene009917:intron | 25.0% |
| ! | TTCATAAATATCGTAATCCA+AGG | + | chr5.3:55488127-55488146 | None:intergenic | 25.0% |
| TTATGTCTGCTCTATTAAGA+AGG | - | chr5.3:55487656-55487675 | MS.gene009917:intron | 30.0% | |
| TTTATAAGCCGATATCTTCT+CGG | - | chr5.3:55488175-55488194 | MS.gene009917:CDS | 30.0% | |
| ! | AGCTTTCATTTTGTTCCAAA+AGG | - | chr5.3:55488101-55488120 | MS.gene009917:CDS | 30.0% |
| !!! | TTTTGGAACAAAATGAAAGC+TGG | + | chr5.3:55488102-55488121 | None:intergenic | 30.0% |
| AAACCTGTTCCAAATCCTTA+TGG | + | chr5.3:55487788-55487807 | None:intergenic | 35.0% | |
| AACCTGTTCCAAATCCTTAT+GGG | + | chr5.3:55487787-55487806 | None:intergenic | 35.0% | |
| GCAAGATAAGATCATATTCG+CGG | + | chr5.3:55487569-55487588 | None:intergenic | 35.0% | |
| TTATAAGCCGATATCTTCTC+GGG | - | chr5.3:55488176-55488195 | MS.gene009917:CDS | 35.0% | |
| AAAGCTGGAATGTTGTACCT+CGG | + | chr5.3:55488087-55488106 | None:intergenic | 40.0% | |
| CTTATCTTGCCTTTGAGTAC+TGG | - | chr5.3:55487579-55487598 | MS.gene009917:CDS | 40.0% | |
| TGTCTGCTCTATTAAGAAGG+AGG | - | chr5.3:55487659-55487678 | MS.gene009917:intron | 40.0% | |
| TGTTCATCTCCAGTACTCAA+AGG | + | chr5.3:55487591-55487610 | None:intergenic | 40.0% | |
| ! | AGATGAACATTTTCACGTCG+CGG | - | chr5.3:55487602-55487621 | MS.gene009917:CDS | 40.0% |
| ! | CATTTTGTTCCAAAAGGCCT+TGG | - | chr5.3:55488107-55488126 | MS.gene009917:CDS | 40.0% |
| ! | TATCGTAATCCAAGGCCTTT+TGG | + | chr5.3:55488119-55488138 | None:intergenic | 40.0% |
| AAGCTGGAATGTTGTACCTC+GGG | + | chr5.3:55488086-55488105 | None:intergenic | 45.0% | |
| AGCGATACCAAGTCTCTGAT+AGG | + | chr5.3:55487745-55487764 | None:intergenic | 45.0% | |
| CATAACCGACGATATTGCGA+CGG | + | chr5.3:55487641-55487660 | None:intergenic | 45.0% | |
| CTCTATTAAGAAGGAGGAGG+AGG | - | chr5.3:55487665-55487684 | MS.gene009917:intron | 45.0% | |
| CTGCTCTATTAAGAAGGAGG+AGG | - | chr5.3:55487662-55487681 | MS.gene009917:intron | 45.0% | |
| CTGTTCCAAATCCTTATGGG+CGG | + | chr5.3:55487784-55487803 | None:intergenic | 45.0% | |
| TTGGTCTCCCGAGAAGATAT+CGG | + | chr5.3:55488186-55488205 | None:intergenic | 45.0% | |
| CTCGCAACCTATCAGAGACT+TGG | - | chr5.3:55487735-55487754 | MS.gene009917:intron | 50.0% | |
| GAAGGAGGAGGAGGTTAAGA+AGG | - | chr5.3:55487674-55487693 | MS.gene009917:intron | 50.0% | |
| ! | AACATTTTCACGTCGCGGTC+AGG | - | chr5.3:55487607-55487626 | MS.gene009917:CDS | 50.0% |
| ! | CGCCCATAAGGATTTGGAAC+AGG | - | chr5.3:55487782-55487801 | MS.gene009917:intron | 50.0% |
| CGCTGAAATCAGTCACGTCG+TGG | - | chr5.3:55487710-55487729 | MS.gene009917:intron | 55.0% | |
| GACTGCCGCCCATAAGGATT+TGG | - | chr5.3:55487776-55487795 | MS.gene009917:intron | 55.0% | |
| GGAGGAGGAGGTTAAGAAGG+AGG | - | chr5.3:55487677-55487696 | MS.gene009917:intron | 55.0% | |
| TTTCACGTCGCGGTCAGGAA+CGG | - | chr5.3:55487612-55487631 | MS.gene009917:CDS | 55.0% | |
| !! | ACGAGCGCTGAGATGTACTC+AGG | + | chr5.3:55488063-55488082 | None:intergenic | 55.0% |
| GCTTGTGACTGCCGCCCATA+AGG | - | chr5.3:55487770-55487789 | MS.gene009917:intron | 60.0% | |
| ACATCTCAGCGCTCGTCCCG+AGG | - | chr5.3:55488067-55488086 | MS.gene009917:CDS | 65.0% | |
| GGCGGCCGTCGCAATATCGT+CGG | - | chr5.3:55487633-55487652 | MS.gene009917:CDS | 65.0% | |
| CACGTCGCGGTCAGGAACGG+CGG | - | chr5.3:55487615-55487634 | MS.gene009917:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 55487513 | 55488209 | 55487513 | ID=MS.gene009917 |
| chr5.3 | mRNA | 55487513 | 55488209 | 55487513 | ID=MS.gene009917.t1;Parent=MS.gene009917 |
| chr5.3 | exon | 55487919 | 55488209 | 55487919 | ID=MS.gene009917.t1.exon1;Parent=MS.gene009917.t1 |
| chr5.3 | CDS | 55487919 | 55488209 | 55487919 | ID=cds.MS.gene009917.t1;Parent=MS.gene009917.t1 |
| chr5.3 | exon | 55487513 | 55487671 | 55487513 | ID=MS.gene009917.t1.exon2;Parent=MS.gene009917.t1 |
| chr5.3 | CDS | 55487513 | 55487671 | 55487513 | ID=cds.MS.gene009917.t1;Parent=MS.gene009917.t1 |
| Gene Sequence |
| Protein sequence |
