AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009962

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009962.t1 MTR_5g068860 95.538 493 19 1 1 490 19 511 0.0 931
MS.gene009962.t1 MTR_1g115415 53.250 400 185 1 82 481 71 468 6.15e-155 451
MS.gene009962.t1 MTR_8g105560 43.128 422 184 4 82 502 49 415 1.60e-112 345
MS.gene009962.t1 MTR_4g070060 27.606 355 249 4 115 463 235 587 1.42e-34 137
MS.gene009962.t1 MTR_4g084630 23.548 310 199 4 155 462 46 319 2.95e-31 124
MS.gene009962.t1 MTR_4g084630 23.182 220 160 3 120 330 43 262 6.48e-17 82.4
MS.gene009962.t1 MTR_2g034600 28.425 292 199 7 185 473 210 494 1.12e-29 122
MS.gene009962.t1 MTR_2g034600 21.986 282 171 7 118 354 211 488 3.23e-13 72.4
MS.gene009962.t1 MTR_2g034600 28.425 292 199 7 185 473 275 559 1.90e-29 122
MS.gene009962.t1 MTR_2g034600 21.986 282 171 7 118 354 276 553 3.00e-13 72.4
MS.gene009962.t1 MTR_2g034600 28.425 292 199 7 185 473 271 555 2.19e-29 122
MS.gene009962.t1 MTR_2g034600 21.986 282 171 7 118 354 272 549 3.55e-13 72.4
MS.gene009962.t1 MTR_5g094610 25.982 331 196 9 150 471 191 481 5.86e-25 108
MS.gene009962.t1 MTR_5g094610 24.153 236 166 5 113 340 221 451 4.75e-12 68.6
MS.gene009962.t1 MTR_6g003960 24.924 329 241 6 135 461 263 587 5.02e-23 103
MS.gene009962.t1 MTR_2g049780 25.379 264 156 7 202 463 82 306 4.47e-17 82.4
MS.gene009962.t1 MTR_5g041630 25.461 271 158 7 190 458 61 289 5.97e-14 73.2
MS.gene009962.t1 MTR_2g067230 23.022 278 167 6 190 466 73 304 1.08e-13 72.8
MS.gene009962.t1 MTR_7g081270 23.774 265 182 7 206 461 26 279 8.10e-12 66.2
MS.gene009962.t1 MTR_3g092710 22.604 407 266 14 98 461 91 491 3.36e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009962.t1 AT4G02990 72.473 465 119 2 34 489 55 519 0.0 675
MS.gene009962.t1 AT4G02990 72.473 465 119 2 34 489 55 519 0.0 675
MS.gene009962.t1 AT2G44020 49.876 403 200 1 82 484 76 476 2.89e-145 427
MS.gene009962.t1 AT2G21710 26.854 391 276 7 114 499 241 626 8.54e-40 153
MS.gene009962.t1 AT2G21710 24.460 278 194 6 81 343 274 550 9.81e-18 87.0
MS.gene009962.t1 AT4G38160 24.638 276 205 3 198 470 54 329 2.08e-32 127
MS.gene009962.t1 AT4G38160 20.705 227 167 5 116 330 39 264 2.40e-14 74.7
MS.gene009962.t1 AT4G38160 24.638 276 205 3 198 470 54 329 2.08e-32 127
MS.gene009962.t1 AT4G38160 20.705 227 167 5 116 330 39 264 2.40e-14 74.7
MS.gene009962.t1 AT4G38160 24.638 276 205 3 198 470 54 329 2.77e-32 127
MS.gene009962.t1 AT4G38160 20.705 227 167 5 116 330 39 264 1.91e-14 75.5
MS.gene009962.t1 AT4G38160 24.638 276 205 3 198 470 54 329 6.28e-32 126
MS.gene009962.t1 AT4G38160 20.705 227 167 5 116 330 39 264 4.07e-14 74.3
MS.gene009962.t1 AT4G14605 29.434 265 177 7 201 462 211 468 3.99e-27 114
MS.gene009962.t1 AT4G14605 29.434 265 177 7 201 462 211 468 3.99e-27 114
MS.gene009962.t1 AT2G21710 25.182 274 203 2 153 425 284 556 3.50e-26 112
MS.gene009962.t1 AT2G21710 24.460 278 194 6 81 343 274 550 1.41e-17 86.3
MS.gene009962.t1 AT2G21710 25.368 272 201 2 153 423 284 554 4.21e-26 112
MS.gene009962.t1 AT2G21710 24.460 278 194 6 81 343 274 550 1.05e-17 86.7
MS.gene009962.t1 AT1G78930 23.404 329 247 5 135 461 249 574 7.66e-25 108
MS.gene009962.t1 AT5G55580 23.529 306 223 7 143 443 38 337 4.98e-20 92.4
MS.gene009962.t1 AT5G55580 23.932 234 167 5 111 337 106 335 1.48e-12 69.7
MS.gene009962.t1 AT5G55580 24.036 337 242 9 143 471 144 474 1.40e-19 92.0
MS.gene009962.t1 AT5G55580 23.932 234 167 5 111 337 212 441 3.18e-12 69.3
MS.gene009962.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene009962.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene009962.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene009962.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene009962.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene009962.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene009962.t1 AT1G61970 25.843 356 245 9 127 473 61 406 4.07e-17 84.0
MS.gene009962.t1 AT2G34620 26.006 323 193 7 145 463 12 292 5.56e-17 82.0
MS.gene009962.t1 AT5G55580 23.711 194 141 4 151 340 185 375 6.56e-14 73.9
MS.gene009962.t1 AT1G62120 22.378 286 186 6 189 441 91 373 4.61e-13 71.6
MS.gene009962.t1 AT1G61980 22.930 314 201 9 189 464 87 397 1.64e-12 69.7
MS.gene009962.t1 AT1G61980 22.930 314 201 9 189 464 87 397 1.64e-12 69.7
MS.gene009962.t1 AT1G61980 22.930 314 201 9 189 464 87 397 1.64e-12 69.7
MS.gene009962.t1 AT3G18870 27.517 149 104 2 314 461 123 268 5.16e-12 67.0
MS.gene009962.t1 AT2G03050 24.510 204 149 5 265 465 71 272 3.10e-11 64.7
MS.gene009962.t1 AT2G03050 24.510 204 149 5 265 465 72 273 3.28e-11 64.7
MS.gene009962.t1 AT5G54180 24.051 395 232 11 94 466 145 493 5.90e-11 65.1

Find 126 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTAGATTCCCGGAGATTT+TGG 0.122651 5.3:-54919563 MS.gene009962:CDS
GCTTGATTACCTTGGTAAAT+TGG 0.181028 5.3:-54919833 MS.gene009962:CDS
GAGACACAACCTTATATTCT+TGG 0.218625 5.3:-54919454 MS.gene009962:CDS
AATTGCCATGTAATTGTATT+AGG 0.259846 5.3:-54920236 MS.gene009962:CDS
TTCCCAGCATTCTTCACTTA+TGG 0.279890 5.3:-54919028 MS.gene009962:CDS
GGAAAGGCCTTTGGTAGATT+TGG 0.285905 5.3:-54919056 MS.gene009962:CDS
TTATATTCTTGGATTCGATT+TGG 0.301999 5.3:-54919443 MS.gene009962:CDS
ACATCTGTTGCTTATTTGAT+TGG 0.305827 5.3:-54919622 MS.gene009962:CDS
GCTCAATTCTGTGCTTGATT+TGG 0.309060 5.3:-54919284 MS.gene009962:CDS
AGCATTCTTCACTTATGGTT+TGG 0.313050 5.3:-54919023 MS.gene009962:CDS
AGTTTGAATCCCAACACTTC+AGG 0.346326 5.3:+54919661 None:intergenic
GCCGGTGGTCAAGTATCTTC+AGG 0.347359 5.3:-54919731 MS.gene009962:CDS
TTCATAACATCAACCGTAAC+AGG 0.355753 5.3:+54919958 None:intergenic
GAAATGCCAATTCTAACATT+TGG 0.356044 5.3:-54920294 MS.gene009962:CDS
GAATGACCAAATGTTAGAAT+TGG 0.365180 5.3:+54920288 None:intergenic
GGACTATGACACTATTGATA+TGG 0.372055 5.3:-54918897 MS.gene009962:CDS
GATTTGGATGTTGAGGATTT+CGG 0.372881 5.3:-54919268 MS.gene009962:CDS
AAGATTCAATGTTCTGTTGC+TGG 0.373247 5.3:-54920195 MS.gene009962:CDS
ACCTTGGTAAATTGGGAGTT+AGG 0.378790 5.3:-54919825 MS.gene009962:CDS
CTTGATTACCTTGGTAAATT+GGG 0.382917 5.3:-54919832 MS.gene009962:CDS
AAATCGAATCCAAGAATATA+AGG 0.400009 5.3:+54919445 None:intergenic
ACCCGCATCCCCAAAATCTC+CGG 0.400611 5.3:+54919553 None:intergenic
TAACAACTATCCTCTTGTAC+TGG 0.404481 5.3:-54919887 MS.gene009962:CDS
TGGCGAAATTCAAAGGAAAC+GGG 0.417191 5.3:-54920115 MS.gene009962:CDS
AGGCATCTTAACTAGATTCC+CGG 0.419846 5.3:-54919572 MS.gene009962:CDS
TGATATGGAAGAGATGGAAA+TGG 0.426007 5.3:-54918882 MS.gene009962:CDS
TATGAGTTCTTGCGAGGAAT+CGG 0.426180 5.3:-54920015 MS.gene009962:CDS
ATGTAGTGTCAAGAAGAATA+TGG 0.433633 5.3:-54919863 MS.gene009962:CDS
GACCGGTGCTGCTGATTCGC+GGG 0.440005 5.3:-54920154 MS.gene009962:CDS
TATCTGCAAGCTTCGGCTCT+AGG 0.443828 5.3:+54919317 None:intergenic
GTAATTGTATTAGGAAGATA+AGG 0.444282 5.3:-54920227 MS.gene009962:CDS
GTGGAGTATCTGGAAAGCTT+GGG 0.446638 5.3:-54919508 MS.gene009962:CDS
GTTGCTTATTTGATTGGAAT+CGG 0.449333 5.3:-54919616 MS.gene009962:CDS
GCTCAGTATCCTGACATCAT+TGG 0.449340 5.3:-54919346 MS.gene009962:CDS
TCAAGACCATCAAGTTCATC+AGG 0.450633 5.3:+54919985 None:intergenic
TGTGGAGTATCTGGAAAGCT+TGG 0.452920 5.3:-54919509 MS.gene009962:CDS
CTGGTAGGACGTATAGTTAT+AGG 0.454647 5.3:-54920176 MS.gene009962:CDS
ATTGTTCCTGATGAACTTGA+TGG 0.456166 5.3:-54919991 MS.gene009962:CDS
AATTTCGCCAGAGTCCCGTT+TGG 0.461902 5.3:+54920128 None:intergenic
CTGGCGAAATTCAAAGGAAA+CGG 0.464517 5.3:-54920116 MS.gene009962:CDS
CCGGTGGTCAAGTATCTTCA+GGG 0.466032 5.3:-54919730 MS.gene009962:CDS
CGGGGATCCAAACGGGACTC+TGG 0.468895 5.3:-54920135 MS.gene009962:CDS
TGATTCGCGGGGATCCAAAC+GGG 0.468998 5.3:-54920142 MS.gene009962:CDS
GAGAGGTATCCTGAAGTGTT+GGG 0.470423 5.3:-54919670 MS.gene009962:CDS
CACTATTGATATGGAAGAGA+TGG 0.470517 5.3:-54918888 MS.gene009962:CDS
GCCGAACATTAAACTCTTCG+AGG 0.470544 5.3:+54919392 None:intergenic
CTTTCCAGATACTCCACAAA+AGG 0.473709 5.3:+54919514 None:intergenic
CTTCTCTTATCTGCAAGCTT+CGG 0.475890 5.3:+54919310 None:intergenic
CATAACATGGTTGCTAAGAA+AGG 0.477153 5.3:-54918980 MS.gene009962:CDS
GCTTCATTCTAGTGTGGTTG+TGG 0.477857 5.3:-54919761 MS.gene009962:CDS
AAACCATAAGTGAAGAATGC+TGG 0.479920 5.3:+54919025 None:intergenic
GAAAGGATTGAAGTGTTCTC+TGG 0.480186 5.3:-54918963 MS.gene009962:CDS
GGACCGGTGCTGCTGATTCG+CGG 0.485919 5.3:-54920155 MS.gene009962:CDS
CCCTCGAAGAGTTTAATGTT+CGG 0.492782 5.3:-54919393 MS.gene009962:CDS
CTGATTCGCGGGGATCCAAA+CGG 0.493660 5.3:-54920143 MS.gene009962:CDS
CAAATGAGGAAGATGATTGT+TGG 0.494500 5.3:-54919148 MS.gene009962:CDS
GTGTTGGGATTCAAACTTGA+GGG 0.498220 5.3:-54919655 MS.gene009962:CDS
AAGTGAGATGGAAAGGCCTT+TGG 0.499414 5.3:-54919065 MS.gene009962:CDS
ATCTGAAATTAAGAAAGATA+AGG 0.501088 5.3:-54920058 MS.gene009962:CDS
AATGAATTCATGTCAAATGA+TGG 0.505787 5.3:+54918857 None:intergenic
GTTCCTGTGCTTGATTACCT+TGG 0.506625 5.3:-54919841 MS.gene009962:CDS
TCAGGGGATGGATATCAAAC+CGG 0.510690 5.3:-54919713 MS.gene009962:CDS
AAGGCCTTTCCATCTCACTT+TGG 0.510909 5.3:+54919068 None:intergenic
TGAGAGGTATCCTGAAGTGT+TGG 0.512556 5.3:-54919671 MS.gene009962:CDS
CCACGTTAACGCAGTTCTTG+CGG 0.514225 5.3:-54919798 MS.gene009962:CDS
TGTTACGGTTGATGTTATGA+AGG 0.514841 5.3:-54919956 MS.gene009962:CDS
TGGTCTTGAACTTCCTGTTA+CGG 0.514972 5.3:-54919971 MS.gene009962:CDS
GAATCTGATTCATCGCTCCT+TGG 0.517997 5.3:+54918827 None:intergenic
TTAGCAACCATGTTATGCCT+AGG 0.519862 5.3:+54918986 None:intergenic
AGTCTAGCAATAGCTAGCCT+TGG 0.521140 5.3:+54919481 None:intergenic
TGTTTAAAGCAAGTAGTTGA+GGG 0.521469 5.3:+54919122 None:intergenic
TGTGCTTGATTTGGATGTTG+AGG 0.522040 5.3:-54919275 MS.gene009962:CDS
AGGTCGGTTCCAATGATGTC+AGG 0.523721 5.3:+54919337 None:intergenic
AACCATAAGTGAAGAATGCT+GGG 0.531139 5.3:+54919026 None:intergenic
AATTTCAGATAAACTCGGAC+GGG 0.532219 5.3:+54920071 None:intergenic
CCCGCATCCCCAAAATCTCC+GGG 0.534445 5.3:+54919554 None:intergenic
ATCTTCCTAATACAATTACA+TGG 0.538869 5.3:+54920231 None:intergenic
TCCCCGCGAATCAGCAGCAC+CGG 0.539690 5.3:+54920152 None:intergenic
TGCAAGCTTCGGCTCTAGGT+CGG 0.547317 5.3:+54919321 None:intergenic
CGATTACTTCCAAAGTGAGA+TGG 0.549323 5.3:-54919077 MS.gene009962:CDS
GGGACTCTGGCGAAATTCAA+AGG 0.550251 5.3:-54920122 MS.gene009962:CDS
TTCTTAATTTCAGATAAACT+CGG 0.551846 5.3:+54920066 None:intergenic
TATTTGATTGGAATCGGTGT+TGG 0.555605 5.3:-54919610 MS.gene009962:CDS
ATGTTTAAAGCAAGTAGTTG+AGG 0.555715 5.3:+54919121 None:intergenic
CCCTGAAGATACTTGACCAC+CGG 0.559238 5.3:+54919730 None:intergenic
AACAACTATCCTCTTGTACT+GGG 0.560648 5.3:-54919886 MS.gene009962:CDS
CCGCAAGAACTGCGTTAACG+TGG 0.564848 5.3:+54919798 None:intergenic
AGGATACTGAGCAATTATAG+AGG 0.570014 5.3:+54919357 None:intergenic
ACACTAGAATGAAGCACTTG+TGG 0.573884 5.3:+54919769 None:intergenic
AAATCAACGTGCTTCAGCAT+AGG 0.578326 5.3:+54919199 None:intergenic
ACACTACATCCCAGTACAAG+AGG 0.579075 5.3:+54919877 None:intergenic
GGTCAAGTATCTTCAGGGGA+TGG 0.581823 5.3:-54919725 MS.gene009962:CDS
TGTGGTTGTGGATCTTGTGC+CGG 0.581899 5.3:-54919749 MS.gene009962:CDS
ACTTCCAAAGTGAGATGGAA+AGG 0.582725 5.3:-54919072 MS.gene009962:CDS
TGAATTCATTGATGCGGCCA+AGG 0.585206 5.3:-54918844 MS.gene009962:CDS
TTGACATGAATTCATTGATG+CGG 0.585388 5.3:-54918850 MS.gene009962:CDS
ATATTCCTCGTGTTCTTGAG+AGG 0.588108 5.3:-54919687 MS.gene009962:CDS
TGAGAAATTTGAGCAACGAA+TGG 0.590339 5.3:-54918918 MS.gene009962:CDS
AGAACACGAGGAATATCATC+CGG 0.593566 5.3:+54919694 None:intergenic
GATTGAAGTGTTCTCTGGCA+TGG 0.595640 5.3:-54918958 MS.gene009962:CDS
ATAAGCAACAGATGTACTCA+TGG 0.595928 5.3:+54919630 None:intergenic
TCCTAACTCCCAATTTACCA+AGG 0.597942 5.3:+54919824 None:intergenic
CCGAACATTAAACTCTTCGA+GGG 0.604717 5.3:+54919393 None:intergenic
AGGACGTATAGTTATAGGAC+CGG 0.612646 5.3:-54920171 MS.gene009962:CDS
TTACCAAGGTAATCAAGCAC+AGG 0.615617 5.3:+54919838 None:intergenic
TTCAATGTTCTGTTGCTGGT+AGG 0.617163 5.3:-54920191 MS.gene009962:CDS
TGGAAAGCTTGGGTATTCCA+AGG 0.618103 5.3:-54919498 MS.gene009962:CDS
AACTCAACCAAATCTACCAA+AGG 0.619874 5.3:+54919049 None:intergenic
TAATTTCAGATAAACTCGGA+CGG 0.623058 5.3:+54920070 None:intergenic
ACAAGTGCTTCATTCTAGTG+TGG 0.623981 5.3:-54919767 MS.gene009962:CDS
ATTGTTTATGAGTTCTTGCG+AGG 0.628501 5.3:-54920021 MS.gene009962:CDS
TGGAGTCAACTATAAAACCT+AGG 0.628708 5.3:-54919003 MS.gene009962:CDS
ACCGGTGCTGCTGATTCGCG+GGG 0.640590 5.3:-54920153 MS.gene009962:CDS
TATAAAACCTAGGCATAACA+TGG 0.645601 5.3:-54918993 MS.gene009962:CDS
AATCATTGAGAAGATGCCGC+AGG 0.648015 5.3:-54919242 MS.gene009962:CDS
GGTGTTGGAAGAAGAGAACT+CGG 0.656801 5.3:-54919595 MS.gene009962:CDS
CGGTGGTCAAGTATCTTCAG+GGG 0.659657 5.3:-54919729 MS.gene009962:CDS
AGTGTTGGGATTCAAACTTG+AGG 0.668806 5.3:-54919656 MS.gene009962:CDS
GGTTGTGGATCTTGTGCCGG+TGG 0.671700 5.3:-54919746 MS.gene009962:CDS
TCAGCATAGGTGTAGTACTG+AGG 0.702066 5.3:+54919212 None:intergenic
ATAAACAATTTCACGCAACG+TGG 0.704806 5.3:+54920035 None:intergenic
GGCGAAATTCAAAGGAAACG+GGG 0.719631 5.3:-54920114 MS.gene009962:CDS
GAAATTCAAAGGAAACGGGG+AGG 0.721546 5.3:-54920111 MS.gene009962:CDS
GTACTGAGGCTAACTACCTG+CGG 0.724109 5.3:+54919226 None:intergenic
GGATACCTCTCAAGAACACG+AGG 0.733931 5.3:+54919682 None:intergenic
GTTGGAAGAAGAGAACTCGG+AGG 0.795387 5.3:-54919592 MS.gene009962:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTTAATTTCAGATAAACT+CGG + chr5.3:54919000-54919019 None:intergenic 20.0%
!!! ATCTGAAATTAAGAAAGATA+AGG - chr5.3:54919005-54919024 MS.gene009962:CDS 20.0%
! AAATCGAATCCAAGAATATA+AGG + chr5.3:54919621-54919640 None:intergenic 25.0%
! AATGAATTCATGTCAAATGA+TGG + chr5.3:54920209-54920228 None:intergenic 25.0%
! AATTGCCATGTAATTGTATT+AGG - chr5.3:54918827-54918846 MS.gene009962:CDS 25.0%
! ATCTTCCTAATACAATTACA+TGG + chr5.3:54918835-54918854 None:intergenic 25.0%
! GTAATTGTATTAGGAAGATA+AGG - chr5.3:54918836-54918855 MS.gene009962:CDS 25.0%
! TTATATTCTTGGATTCGATT+TGG - chr5.3:54919620-54919639 MS.gene009962:CDS 25.0%
!! TTGATTTTCTTAAGGATTGT+GGG - chr5.3:54919880-54919899 MS.gene009962:CDS 25.0%
ATGTAGTGTCAAGAAGAATA+TGG - chr5.3:54919200-54919219 MS.gene009962:CDS 30.0%
CTTGATTACCTTGGTAAATT+GGG - chr5.3:54919231-54919250 MS.gene009962:CDS 30.0%
GAATGACCAAATGTTAGAAT+TGG + chr5.3:54918778-54918797 None:intergenic 30.0%
GTTGCTTATTTGATTGGAAT+CGG - chr5.3:54919447-54919466 MS.gene009962:CDS 30.0%
TAATTTCAGATAAACTCGGA+CGG + chr5.3:54918996-54919015 None:intergenic 30.0%
TATAAAACCTAGGCATAACA+TGG - chr5.3:54920070-54920089 MS.gene009962:CDS 30.0%
TTGACATGAATTCATTGATG+CGG - chr5.3:54920213-54920232 MS.gene009962:CDS 30.0%
! ACATCTGTTGCTTATTTGAT+TGG - chr5.3:54919441-54919460 MS.gene009962:CDS 30.0%
! ATGTTTAAAGCAAGTAGTTG+AGG + chr5.3:54919945-54919964 None:intergenic 30.0%
! TGTTTAAAGCAAGTAGTTGA+GGG + chr5.3:54919944-54919963 None:intergenic 30.0%
! TTGATTTTCTTCACAGTTTG+GGG - chr5.3:54919136-54919155 MS.gene009962:CDS 30.0%
!! GTTGATTTTCTTAAGGATTG+TGG - chr5.3:54919879-54919898 MS.gene009962:CDS 30.0%
!! GTTGATTTTCTTCACAGTTT+GGG - chr5.3:54919135-54919154 MS.gene009962:CDS 30.0%
!!! TGTTGATTTTCTTCACAGTT+TGG - chr5.3:54919134-54919153 MS.gene009962:CDS 30.0%
AAACCATAAGTGAAGAATGC+TGG + chr5.3:54920041-54920060 None:intergenic 35.0%
AACAACTATCCTCTTGTACT+GGG - chr5.3:54919177-54919196 MS.gene009962:CDS 35.0%
AACCATAAGTGAAGAATGCT+GGG + chr5.3:54920040-54920059 None:intergenic 35.0%
AACTCAACCAAATCTACCAA+AGG + chr5.3:54920017-54920036 None:intergenic 35.0%
AAGATTCAATGTTCTGTTGC+TGG - chr5.3:54918868-54918887 MS.gene009962:CDS 35.0%
AATTTCAGATAAACTCGGAC+GGG + chr5.3:54918995-54919014 None:intergenic 35.0%
AGCATTCTTCACTTATGGTT+TGG - chr5.3:54920040-54920059 MS.gene009962:CDS 35.0%
AGGATACTGAGCAATTATAG+AGG + chr5.3:54919709-54919728 None:intergenic 35.0%
ATAAACAATTTCACGCAACG+TGG + chr5.3:54919031-54919050 None:intergenic 35.0%
ATAAGCAACAGATGTACTCA+TGG + chr5.3:54919436-54919455 None:intergenic 35.0%
ATTGTTCCTGATGAACTTGA+TGG - chr5.3:54919072-54919091 MS.gene009962:CDS 35.0%
ATTGTTTATGAGTTCTTGCG+AGG - chr5.3:54919042-54919061 MS.gene009962:CDS 35.0%
CAAATGAGGAAGATGATTGT+TGG - chr5.3:54919915-54919934 MS.gene009962:CDS 35.0%
CACTATTGATATGGAAGAGA+TGG - chr5.3:54920175-54920194 MS.gene009962:CDS 35.0%
CATAACATGGTTGCTAAGAA+AGG - chr5.3:54920083-54920102 MS.gene009962:CDS 35.0%
GAGACACAACCTTATATTCT+TGG - chr5.3:54919609-54919628 MS.gene009962:CDS 35.0%
GATTTGGATGTTGAGGATTT+CGG - chr5.3:54919795-54919814 MS.gene009962:CDS 35.0%
GCTTGATTACCTTGGTAAAT+TGG - chr5.3:54919230-54919249 MS.gene009962:CDS 35.0%
GGACTATGACACTATTGATA+TGG - chr5.3:54920166-54920185 MS.gene009962:CDS 35.0%
TAACAACTATCCTCTTGTAC+TGG - chr5.3:54919176-54919195 MS.gene009962:CDS 35.0%
TATTTGATTGGAATCGGTGT+TGG - chr5.3:54919453-54919472 MS.gene009962:CDS 35.0%
TGAGAAATTTGAGCAACGAA+TGG - chr5.3:54920145-54920164 MS.gene009962:CDS 35.0%
TGATATGGAAGAGATGGAAA+TGG - chr5.3:54920181-54920200 MS.gene009962:CDS 35.0%
TGGAGTCAACTATAAAACCT+AGG - chr5.3:54920060-54920079 MS.gene009962:CDS 35.0%
TTCATAACATCAACCGTAAC+AGG + chr5.3:54919108-54919127 None:intergenic 35.0%
! TGTTACGGTTGATGTTATGA+AGG - chr5.3:54919107-54919126 MS.gene009962:CDS 35.0%
!!! GAAGCACGTTGATTTTCTTA+AGG - chr5.3:54919872-54919891 MS.gene009962:CDS 35.0%
AAATCAACGTGCTTCAGCAT+AGG + chr5.3:54919867-54919886 None:intergenic 40.0%
ACAAGTGCTTCATTCTAGTG+TGG - chr5.3:54919296-54919315 MS.gene009962:CDS 40.0%
ACCTTGGTAAATTGGGAGTT+AGG - chr5.3:54919238-54919257 MS.gene009962:CDS 40.0%
ACTTCCAAAGTGAGATGGAA+AGG - chr5.3:54919991-54920010 MS.gene009962:CDS 40.0%
AGAACACGAGGAATATCATC+CGG + chr5.3:54919372-54919391 None:intergenic 40.0%
AGGACGTATAGTTATAGGAC+CGG - chr5.3:54918892-54918911 MS.gene009962:CDS 40.0%
AGGCATCTTAACTAGATTCC+CGG - chr5.3:54919491-54919510 MS.gene009962:CDS 40.0%
AGTTTGAATCCCAACACTTC+AGG + chr5.3:54919405-54919424 None:intergenic 40.0%
ATATTCCTCGTGTTCTTGAG+AGG - chr5.3:54919376-54919395 MS.gene009962:CDS 40.0%
CCCTCGAAGAGTTTAATGTT+CGG - chr5.3:54919670-54919689 MS.gene009962:CDS 40.0%
CCGAACATTAAACTCTTCGA+GGG + chr5.3:54919673-54919692 None:intergenic 40.0%
CGATTACTTCCAAAGTGAGA+TGG - chr5.3:54919986-54920005 MS.gene009962:CDS 40.0%
CTGGCGAAATTCAAAGGAAA+CGG - chr5.3:54918947-54918966 MS.gene009962:CDS 40.0%
CTGGTAGGACGTATAGTTAT+AGG - chr5.3:54918887-54918906 MS.gene009962:CDS 40.0%
CTTCTCTTATCTGCAAGCTT+CGG + chr5.3:54919756-54919775 None:intergenic 40.0%
CTTTCCAGATACTCCACAAA+AGG + chr5.3:54919552-54919571 None:intergenic 40.0%
GCTCAATTCTGTGCTTGATT+TGG - chr5.3:54919779-54919798 MS.gene009962:CDS 40.0%
GTGTTGGGATTCAAACTTGA+GGG - chr5.3:54919408-54919427 MS.gene009962:CDS 40.0%
TATGAGTTCTTGCGAGGAAT+CGG - chr5.3:54919048-54919067 MS.gene009962:CDS 40.0%
TCAAGACCATCAAGTTCATC+AGG + chr5.3:54919081-54919100 None:intergenic 40.0%
TCCTAACTCCCAATTTACCA+AGG + chr5.3:54919242-54919261 None:intergenic 40.0%
TGGCGAAATTCAAAGGAAAC+GGG - chr5.3:54918948-54918967 MS.gene009962:CDS 40.0%
TTACCAAGGTAATCAAGCAC+AGG + chr5.3:54919228-54919247 None:intergenic 40.0%
TTAGCAACCATGTTATGCCT+AGG + chr5.3:54920080-54920099 None:intergenic 40.0%
TTCAATGTTCTGTTGCTGGT+AGG - chr5.3:54918872-54918891 MS.gene009962:CDS 40.0%
TTCCCAGCATTCTTCACTTA+TGG - chr5.3:54920035-54920054 MS.gene009962:CDS 40.0%
! AACTAGATTCCCGGAGATTT+TGG - chr5.3:54919500-54919519 MS.gene009962:CDS 40.0%
! ACACTAGAATGAAGCACTTG+TGG + chr5.3:54919297-54919316 None:intergenic 40.0%
! ACTAGATTCCCGGAGATTTT+GGG - chr5.3:54919501-54919520 MS.gene009962:CDS 40.0%
! AGTGTTGGGATTCAAACTTG+AGG - chr5.3:54919407-54919426 MS.gene009962:CDS 40.0%
! GAAAGGATTGAAGTGTTCTC+TGG - chr5.3:54920100-54920119 MS.gene009962:CDS 40.0%
! GGTTTTCTGTGGATCAAATG+AGG - chr5.3:54919901-54919920 MS.gene009962:CDS 40.0%
! TAAGGATTGTGGGTTTTCTG+TGG - chr5.3:54919890-54919909 MS.gene009962:CDS 40.0%
! TAGAGTCATCAAGCCTTTTG+TGG - chr5.3:54919536-54919555 MS.gene009962:CDS 40.0%
! TGGTCTTGAACTTCCTGTTA+CGG - chr5.3:54919092-54919111 MS.gene009962:CDS 40.0%
!! TGTGCTTGATTTGGATGTTG+AGG - chr5.3:54919788-54919807 MS.gene009962:CDS 40.0%
AAGGCCTTTCCATCTCACTT+TGG + chr5.3:54919998-54920017 None:intergenic 45.0%
AAGTGAGATGGAAAGGCCTT+TGG - chr5.3:54919998-54920017 MS.gene009962:CDS 45.0%
AATCATTGAGAAGATGCCGC+AGG - chr5.3:54919821-54919840 MS.gene009962:CDS 45.0%
ACACTACATCCCAGTACAAG+AGG + chr5.3:54919189-54919208 None:intergenic 45.0%
GAAATTCAAAGGAAACGGGG+AGG - chr5.3:54918952-54918971 MS.gene009962:CDS 45.0%
GAATCTGATTCATCGCTCCT+TGG + chr5.3:54920239-54920258 None:intergenic 45.0%
GCCGAACATTAAACTCTTCG+AGG + chr5.3:54919674-54919693 None:intergenic 45.0%
GCTCAGTATCCTGACATCAT+TGG - chr5.3:54919717-54919736 MS.gene009962:CDS 45.0%
GCTTCATTCTAGTGTGGTTG+TGG - chr5.3:54919302-54919321 MS.gene009962:CDS 45.0%
GGAAAGGCCTTTGGTAGATT+TGG - chr5.3:54920007-54920026 MS.gene009962:CDS 45.0%
GGCGAAATTCAAAGGAAACG+GGG - chr5.3:54918949-54918968 MS.gene009962:CDS 45.0%
GTTCCTGTGCTTGATTACCT+TGG - chr5.3:54919222-54919241 MS.gene009962:CDS 45.0%
TCAGCATAGGTGTAGTACTG+AGG + chr5.3:54919854-54919873 None:intergenic 45.0%
TCAGGGGATGGATATCAAAC+CGG - chr5.3:54919350-54919369 MS.gene009962:CDS 45.0%
TGAATTCATTGATGCGGCCA+AGG - chr5.3:54920219-54920238 MS.gene009962:CDS 45.0%
TGGAAAGCTTGGGTATTCCA+AGG - chr5.3:54919565-54919584 MS.gene009962:CDS 45.0%
! AGTCTAGCAATAGCTAGCCT+TGG + chr5.3:54919585-54919604 None:intergenic 45.0%
! CTAGATTCCCGGAGATTTTG+GGG - chr5.3:54919502-54919521 MS.gene009962:CDS 45.0%
! GATTGAAGTGTTCTCTGGCA+TGG - chr5.3:54920105-54920124 MS.gene009962:CDS 45.0%
! GGTGTTGGAAGAAGAGAACT+CGG - chr5.3:54919468-54919487 MS.gene009962:CDS 45.0%
! GTGGAGTATCTGGAAAGCTT+GGG - chr5.3:54919555-54919574 MS.gene009962:CDS 45.0%
! TGTGGAGTATCTGGAAAGCT+TGG - chr5.3:54919554-54919573 MS.gene009962:CDS 45.0%
!! CAAGCCTTTTGTGGAGTATC+TGG - chr5.3:54919545-54919564 MS.gene009962:CDS 45.0%
!! GAGAGGTATCCTGAAGTGTT+GGG - chr5.3:54919393-54919412 MS.gene009962:CDS 45.0%
!! TGAGAGGTATCCTGAAGTGT+TGG - chr5.3:54919392-54919411 MS.gene009962:CDS 45.0%
AATTTCGCCAGAGTCCCGTT+TGG + chr5.3:54918938-54918957 None:intergenic 50.0%
CCACGTTAACGCAGTTCTTG+CGG - chr5.3:54919265-54919284 MS.gene009962:CDS 50.0%
CCCTGAAGATACTTGACCAC+CGG + chr5.3:54919336-54919355 None:intergenic 50.0%
CCGGTGGTCAAGTATCTTCA+GGG - chr5.3:54919333-54919352 MS.gene009962:CDS 50.0%
CGGTGGTCAAGTATCTTCAG+GGG - chr5.3:54919334-54919353 MS.gene009962:CDS 50.0%
GGATACCTCTCAAGAACACG+AGG + chr5.3:54919384-54919403 None:intergenic 50.0%
GGGACTCTGGCGAAATTCAA+AGG - chr5.3:54918941-54918960 MS.gene009962:CDS 50.0%
GGTCAAGTATCTTCAGGGGA+TGG - chr5.3:54919338-54919357 MS.gene009962:CDS 50.0%
GTACTGAGGCTAACTACCTG+CGG + chr5.3:54919840-54919859 None:intergenic 50.0%
GTTGGAAGAAGAGAACTCGG+AGG - chr5.3:54919471-54919490 MS.gene009962:CDS 50.0%
TATCTGCAAGCTTCGGCTCT+AGG + chr5.3:54919749-54919768 None:intergenic 50.0%
TGTGGTTGTGGATCTTGTGC+CGG - chr5.3:54919314-54919333 MS.gene009962:CDS 50.0%
! AGGTCGGTTCCAATGATGTC+AGG + chr5.3:54919729-54919748 None:intergenic 50.0%
ACCCGCATCCCCAAAATCTC+CGG + chr5.3:54919513-54919532 None:intergenic 55.0%
CCGCAAGAACTGCGTTAACG+TGG + chr5.3:54919268-54919287 None:intergenic 55.0%
GCCGGTGGTCAAGTATCTTC+AGG - chr5.3:54919332-54919351 MS.gene009962:CDS 55.0%
TGATTCGCGGGGATCCAAAC+GGG - chr5.3:54918921-54918940 MS.gene009962:CDS 55.0%
! CTGATTCGCGGGGATCCAAA+CGG - chr5.3:54918920-54918939 MS.gene009962:CDS 55.0%
!! GAGATTTTGGGGATGCGGGT+TGG - chr5.3:54919513-54919532 MS.gene009962:CDS 55.0%
!! TCCCGGAGATTTTGGGGATG+CGG - chr5.3:54919508-54919527 MS.gene009962:CDS 55.0%
!! TGCAAGCTTCGGCTCTAGGT+CGG + chr5.3:54919745-54919764 None:intergenic 55.0%
CCCGCATCCCCAAAATCTCC+GGG + chr5.3:54919512-54919531 None:intergenic 60.0%
GGTTGTGGATCTTGTGCCGG+TGG - chr5.3:54919317-54919336 MS.gene009962:CDS 60.0%
!! CCCGGAGATTTTGGGGATGC+GGG - chr5.3:54919509-54919528 MS.gene009962:CDS 60.0%
CGGGGATCCAAACGGGACTC+TGG - chr5.3:54918928-54918947 MS.gene009962:CDS 65.0%
TCCCCGCGAATCAGCAGCAC+CGG + chr5.3:54918914-54918933 None:intergenic 65.0%
!! ACCGGTGCTGCTGATTCGCG+GGG - chr5.3:54918910-54918929 MS.gene009962:CDS 65.0%
!! GACCGGTGCTGCTGATTCGC+GGG - chr5.3:54918909-54918928 MS.gene009962:CDS 65.0%
!! GGACCGGTGCTGCTGATTCG+CGG - chr5.3:54918908-54918927 MS.gene009962:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 54918772 54920313 54918772 ID=MS.gene009962
chr5.3 mRNA 54918772 54920313 54918772 ID=MS.gene009962.t1;Parent=MS.gene009962
chr5.3 exon 54918772 54920313 54918772 ID=MS.gene009962.t1.exon1;Parent=MS.gene009962.t1
chr5.3 CDS 54918772 54920313 54918772 ID=cds.MS.gene009962.t1;Parent=MS.gene009962.t1
Gene Sequence

>MS.gene009962

ATGCCAATTCTAACATTTGGTCATTCTAAGTTAACTGCTGCTACACTAGCTCTTCAATTGCCATGTAATTGTATTAGGAAGATAAGGTTTATTACAAAGATTCAATGTTCTGTTGCTGGTAGGACGTATAGTTATAGGACCGGTGCTGCTGATTCGCGGGGATCCAAACGGGACTCTGGCGAAATTCAAAGGAAACGGGGAGGTGCATCGTCGTTGTATGCCCGTCCGAGTTTATCTGAAATTAAGAAAGATAAGGCCACGTTGCGTGAAATTGTTTATGAGTTCTTGCGAGGAATCGGCATTGTTCCTGATGAACTTGATGGTCTTGAACTTCCTGTTACGGTTGATGTTATGAAGGAGCGTGTTGATTTTCTTCACAGTTTGGGGCTTACGATTGAAGACATTAACAACTATCCTCTTGTACTGGGATGTAGTGTCAAGAAGAATATGGTTCCTGTGCTTGATTACCTTGGTAAATTGGGAGTTAGGAAATCCACGTTAACGCAGTTCTTGCGGAGATATCCACAAGTGCTTCATTCTAGTGTGGTTGTGGATCTTGTGCCGGTGGTCAAGTATCTTCAGGGGATGGATATCAAACCGGATGATATTCCTCGTGTTCTTGAGAGGTATCCTGAAGTGTTGGGATTCAAACTTGAGGGAACCATGAGTACATCTGTTGCTTATTTGATTGGAATCGGTGTTGGAAGAAGAGAACTCGGAGGCATCTTAACTAGATTCCCGGAGATTTTGGGGATGCGGGTTGGTAGAGTCATCAAGCCTTTTGTGGAGTATCTGGAAAGCTTGGGTATTCCAAGGCTAGCTATTGCTAGACTGATAGAGACACAACCTTATATTCTTGGATTCGATTTGGACGAGAAAGTGAAGTCAAATGTCAAATCCCTCGAAGAGTTTAATGTTCGGCAGACATTGCTTGCCTCTATAATTGCTCAGTATCCTGACATCATTGGAACCGACCTAGAGCCGAAGCTTGCAGATAAGAGAAGTGTGCTCAATTCTGTGCTTGATTTGGATGTTGAGGATTTCGGCTTAATCATTGAGAAGATGCCGCAGGTAGTTAGCCTCAGTACTACACCTATGCTGAAGCACGTTGATTTTCTTAAGGATTGTGGGTTTTCTGTGGATCAAATGAGGAAGATGATTGTTGGATGCCCTCAACTACTTGCTTTAAACATTGATATCATGAAACTTAGCTTCGATTACTTCCAAAGTGAGATGGAAAGGCCTTTGGTAGATTTGGTTGAGTTCCCAGCATTCTTCACTTATGGTTTGGAGTCAACTATAAAACCTAGGCATAACATGGTTGCTAAGAAAGGATTGAAGTGTTCTCTGGCATGGATGCTTAATTGTTCTGATGAGAAATTTGAGCAACGAATGGACTATGACACTATTGATATGGAAGAGATGGAAATGGAACCATCATTTGACATGAATTCATTGATGCGGCCAAGGAGCGATGAATCAGATTCTGATTATGAAGACAGTGATTATGATGATGATGATGATGATGATGATGATGAGTAA
Protein sequence

>MS.gene009962.t1

MPILTFGHSKLTAATLALQLPCNCIRKIRFITKIQCSVAGRTYSYRTGAADSRGSKRDSGEIQRKRGGASSLYARPSLSEIKKDKATLREIVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLHSLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTLTQFLRRYPQVLHSSVVVDLVPVVKYLQGMDIKPDDIPRVLERYPEVLGFKLEGTMSTSVAYLIGIGVGRRELGGILTRFPEILGMRVGRVIKPFVEYLESLGIPRLAIARLIETQPYILGFDLDEKVKSNVKSLEEFNVRQTLLASIIAQYPDIIGTDLEPKLADKRSVLNSVLDLDVEDFGLIIEKMPQVVSLSTTPMLKHVDFLKDCGFSVDQMRKMIVGCPQLLALNIDIMKLSFDYFQSEMERPLVDLVEFPAFFTYGLESTIKPRHNMVAKKGLKCSLAWMLNCSDEKFEQRMDYDTIDMEEMEMEPSFDMNSLMRPRSDESDSDYEDSDYDDDDDDDDDE