AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010021

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010021.t1 MTR_5g067680 93.629 361 18 3 1 360 1 357 0.0 678
MS.gene010021.t1 MTR_5g073620 54.783 115 50 2 164 276 629 743 3.86e-32 128
MS.gene010021.t1 MTR_3g090860 53.763 93 41 2 174 264 100 192 5.66e-24 101
MS.gene010021.t1 MTR_8g099350 59.459 74 30 0 201 274 128 201 1.06e-23 100
MS.gene010021.t1 MTR_7g117200 40.299 134 71 2 198 330 111 236 7.07e-23 98.2
MS.gene010021.t1 MTR_8g027860 59.091 66 27 0 205 270 136 201 3.91e-20 90.5
MS.gene010021.t1 MTR_7g073430 41.667 108 57 2 162 264 75 181 4.67e-18 83.6
MS.gene010021.t1 MTR_7g073380 37.963 108 62 1 162 264 75 182 1.80e-17 82.0
MS.gene010021.t1 MTR_8g005750 52.239 67 32 0 200 266 98 164 4.09e-17 80.9
MS.gene010021.t1 MTR_3g093830 36.975 119 60 3 161 271 82 193 5.16e-17 81.3
MS.gene010021.t1 MTR_2g016770 42.169 83 46 1 180 262 10 90 1.19e-16 78.2
MS.gene010021.t1 MTR_0002s1250 47.692 65 34 0 200 264 106 170 2.90e-16 78.2
MS.gene010021.t1 MTR_2g016770 43.210 81 44 1 182 262 4 82 3.46e-16 77.0
MS.gene010021.t1 MTR_5g043880 42.857 105 53 4 170 273 90 188 4.06e-16 78.2
MS.gene010021.t1 MTR_2g075610 50.000 64 32 0 205 268 29 92 4.56e-16 75.9
MS.gene010021.t1 MTR_2g075750 49.231 65 33 0 205 269 28 92 1.10e-15 74.7
MS.gene010021.t1 MTR_7g079010 54.688 64 24 2 205 266 141 201 1.18e-15 75.5
MS.gene010021.t1 MTR_2g075680 53.968 63 29 0 205 267 148 210 1.24e-15 77.0
MS.gene010021.t1 MTR_7g028415 56.897 58 22 1 205 262 93 147 1.66e-15 73.9
MS.gene010021.t1 MTR_7g028710 56.897 58 22 1 205 262 93 147 1.66e-15 73.9
MS.gene010021.t1 MTR_1g009613 43.182 88 44 3 178 262 233 317 1.66e-15 77.0
MS.gene010021.t1 MTR_8g092140 52.542 59 25 1 205 263 160 215 1.85e-15 75.5
MS.gene010021.t1 MTR_2g032030 39.796 98 53 2 169 262 227 322 2.47e-15 77.0
MS.gene010021.t1 MTR_4g127040 47.059 85 41 3 179 262 219 300 2.71e-15 76.3
MS.gene010021.t1 MTR_2g032030 39.394 99 54 2 168 262 226 322 2.90e-15 77.0
MS.gene010021.t1 MTR_3g009470 52.459 61 25 2 202 262 277 333 3.42e-15 77.0
MS.gene010021.t1 MTR_3g009470 51.515 66 26 3 197 262 274 333 4.34e-15 77.0
MS.gene010021.t1 MTR_4g094908 45.882 85 42 3 178 262 241 321 6.51e-15 75.1
MS.gene010021.t1 MTR_5g067700 45.588 68 37 0 206 273 107 174 8.82e-15 73.6
MS.gene010021.t1 MTR_7g100510 49.275 69 29 3 195 262 179 242 9.07e-15 75.5
MS.gene010021.t1 MTR_1g070455 45.122 82 40 3 194 273 281 359 1.60e-14 74.3
MS.gene010021.t1 MTR_2g075700 43.902 82 45 1 187 268 127 207 1.66e-14 73.9
MS.gene010021.t1 MTR_3g108960 54.237 59 25 1 204 262 313 369 1.66e-14 74.7
MS.gene010021.t1 MTR_8g087000 47.692 65 32 1 198 262 70 132 1.94e-14 72.8
MS.gene010021.t1 MTR_3g095080 53.448 58 24 1 205 262 168 222 1.95e-14 72.4
MS.gene010021.t1 MTR_8g087000 47.692 65 32 1 198 262 69 131 2.28e-14 72.4
MS.gene010021.t1 MTR_5g091390 54.098 61 25 1 205 265 157 214 2.61e-14 73.2
MS.gene010021.t1 MTR_3g095080 53.448 58 24 1 205 262 144 198 2.65e-14 71.6
MS.gene010021.t1 MTR_7g011820 48.387 62 28 2 201 262 224 281 3.01e-14 74.3
MS.gene010021.t1 MTR_7g011820 48.276 58 27 1 205 262 410 464 9.93e-12 66.6
MS.gene010021.t1 MTR_0117s0040 54.237 59 25 1 204 262 73 129 3.25e-14 72.0
MS.gene010021.t1 MTR_8g096730 50.847 59 26 1 205 263 70 125 3.33e-14 69.7
MS.gene010021.t1 MTR_7g062220 52.542 59 25 1 205 263 124 179 3.65e-14 72.8
MS.gene010021.t1 MTR_4g094588 55.172 58 23 1 205 262 178 232 4.90e-14 72.8
MS.gene010021.t1 MTR_5g016610 42.353 85 45 3 179 262 173 254 6.16e-14 71.6
MS.gene010021.t1 MTR_2g083870 40.566 106 51 4 174 274 47 145 6.41e-14 71.2
MS.gene010021.t1 MTR_5g042920 47.059 68 32 2 195 262 141 204 8.86e-14 72.4
MS.gene010021.t1 MTR_5g042920 46.552 58 28 1 205 262 320 374 6.31e-11 63.9
MS.gene010021.t1 MTR_8g007270 53.448 58 24 1 205 262 154 208 1.10e-13 70.1
MS.gene010021.t1 MTR_2g075690 49.206 63 32 0 205 267 141 203 1.60e-13 70.9
MS.gene010021.t1 MTR_7g080020 50.000 58 25 2 205 262 280 333 7.14e-13 70.1
MS.gene010021.t1 MTR_2g024350 49.153 59 28 1 204 262 7 63 8.18e-13 67.8
MS.gene010021.t1 MTR_7g109800 40.404 99 49 3 205 296 145 240 8.21e-13 68.6
MS.gene010021.t1 MTR_5g094430 48.276 58 27 1 205 262 364 418 1.06e-12 69.3
MS.gene010021.t1 MTR_3g056100 48.276 58 27 1 205 262 402 456 1.34e-12 69.3
MS.gene010021.t1 MTR_3g031220 46.377 69 31 3 195 262 188 251 1.34e-12 68.6
MS.gene010021.t1 MTR_5g094430 48.276 58 27 1 205 262 230 284 1.35e-12 68.9
MS.gene010021.t1 MTR_3g056100 48.276 58 27 1 205 262 415 469 1.36e-12 69.3
MS.gene010021.t1 MTR_5g094430 48.276 58 27 1 205 262 252 306 1.42e-12 68.9
MS.gene010021.t1 MTR_3g056100 48.276 58 27 1 205 262 258 312 1.92e-12 68.6
MS.gene010021.t1 MTR_3g056100 48.276 58 27 1 205 262 271 325 1.93e-12 68.6
MS.gene010021.t1 MTR_3g031220 36.697 109 53 4 195 302 188 281 2.05e-12 68.6
MS.gene010021.t1 MTR_3g031220 46.552 58 28 1 205 262 365 419 6.84e-11 63.9
MS.gene010021.t1 MTR_1g086790 51.724 58 25 1 205 262 158 212 2.07e-12 67.8
MS.gene010021.t1 MTR_8g069935 42.391 92 48 3 171 262 300 386 2.59e-12 68.2
MS.gene010021.t1 MTR_5g074400 48.276 58 26 2 205 262 243 296 2.77e-12 68.2
MS.gene010021.t1 MTR_5g074400 48.276 58 27 1 205 262 420 474 8.53e-12 66.6
MS.gene010021.t1 MTR_4g082580 35.106 94 50 2 199 292 139 221 3.90e-12 67.4
MS.gene010021.t1 MTR_4g082580 48.276 58 27 1 205 262 278 332 1.97e-11 65.1
MS.gene010021.t1 MTR_2g088000 53.448 58 25 1 206 263 350 405 4.02e-12 67.8
MS.gene010021.t1 MTR_7g071120 44.928 69 34 2 194 262 155 219 6.31e-12 67.0
MS.gene010021.t1 MTR_3g053330 50.877 57 26 1 206 262 252 306 7.01e-12 66.6
MS.gene010021.t1 MTR_4g132430 46.575 73 27 2 206 278 262 322 7.12e-12 67.0
MS.gene010021.t1 MTR_4g132430 46.575 73 27 2 206 278 128 188 7.20e-12 66.2
MS.gene010021.t1 MTR_4g013260 44.828 58 29 1 205 262 184 238 7.33e-12 66.6
MS.gene010021.t1 MTR_2g033820 44.737 76 33 2 205 280 203 269 7.70e-12 66.2
MS.gene010021.t1 MTR_1g013760 49.123 57 27 1 206 262 141 195 8.60e-12 65.5
MS.gene010021.t1 MTR_2g023930 52.632 57 25 1 206 262 306 360 9.37e-12 66.6
MS.gene010021.t1 MTR_7g110720 52.632 57 25 1 206 262 217 271 1.14e-11 66.2
MS.gene010021.t1 MTR_7g110720 52.632 57 25 1 206 262 190 244 1.22e-11 65.9
MS.gene010021.t1 MTR_2g024380 47.458 59 29 1 204 262 250 306 1.29e-11 65.9
MS.gene010021.t1 MTR_8g098945 52.632 57 25 1 206 262 130 184 1.38e-11 64.7
MS.gene010021.t1 MTR_8g067650 50.000 58 27 1 205 262 339 394 1.89e-11 65.5
MS.gene010021.t1 MTR_3g106060 44.615 65 34 1 198 262 131 193 1.95e-11 64.3
MS.gene010021.t1 MTR_8g067635 50.000 58 27 1 205 262 349 404 2.12e-11 65.5
MS.gene010021.t1 MTR_8g028565 52.632 57 25 1 206 262 156 210 2.28e-11 64.7
MS.gene010021.t1 MTR_4g130900 47.368 57 27 1 206 262 360 413 2.95e-11 65.1
MS.gene010021.t1 MTR_1g099600 49.123 57 27 1 206 262 186 240 4.22e-11 63.5
MS.gene010021.t1 MTR_5g097490 47.368 57 27 1 206 262 253 306 5.34e-11 63.9
MS.gene010021.t1 MTR_8g032510 43.333 60 34 0 205 264 119 178 5.41e-11 63.2
MS.gene010021.t1 MTR_7g079040 41.379 87 42 3 176 262 132 209 5.86e-11 63.5
MS.gene010021.t1 MTR_4g107970 52.632 57 25 1 206 262 179 233 8.05e-11 63.5
MS.gene010021.t1 MTR_7g109600 47.458 59 29 1 204 262 52 108 9.28e-11 62.0
MS.gene010021.t1 MTR_4g107970 52.632 57 25 1 206 262 256 310 9.79e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010021.t1 AT2G40740 34.659 352 148 11 1 346 11 286 1.48e-51 173
MS.gene010021.t1 AT2G40740 34.659 352 148 11 1 346 20 295 3.05e-51 173
MS.gene010021.t1 AT4G11070 52.174 92 42 2 181 271 85 175 2.77e-22 95.5
MS.gene010021.t1 AT4G11070 52.174 92 42 2 181 271 117 207 4.31e-22 95.5
MS.gene010021.t1 AT4G23810 59.420 69 28 0 201 269 154 222 6.40e-22 95.5
MS.gene010021.t1 AT5G24110 45.918 98 52 1 178 275 87 183 2.27e-21 93.6
MS.gene010021.t1 AT2G40740 42.188 128 42 5 221 346 145 242 4.30e-21 91.7
MS.gene010021.t1 AT2G46400 36.429 140 80 4 149 288 56 186 6.79e-20 89.0
MS.gene010021.t1 AT3G56400 52.174 69 33 0 205 273 120 188 3.37e-19 87.0
MS.gene010021.t1 AT1G66550 53.968 63 29 0 202 264 100 162 2.72e-18 83.6
MS.gene010021.t1 AT1G66550 53.968 63 29 0 202 264 105 167 3.46e-18 83.6
MS.gene010021.t1 AT1G66560 43.956 91 44 1 174 264 79 162 6.92e-18 82.4
MS.gene010021.t1 AT1G64000 56.897 58 22 1 205 262 114 168 6.78e-17 78.6
MS.gene010021.t1 AT2G40750 53.333 60 28 0 205 264 152 211 2.86e-16 79.3
MS.gene010021.t1 AT5G41570 56.897 58 22 1 205 262 98 152 2.97e-16 76.3
MS.gene010021.t1 AT4G31550 44.048 84 44 2 179 262 220 300 3.88e-16 79.0
MS.gene010021.t1 AT5G01900 54.237 59 27 0 206 264 111 169 4.28e-16 77.8
MS.gene010021.t1 AT4G31550 48.235 85 40 3 178 262 221 301 4.96e-16 78.6
MS.gene010021.t1 AT2G30590 32.639 144 89 5 123 262 229 368 8.79e-16 78.2
MS.gene010021.t1 AT1G29280 44.086 93 41 4 176 262 42 129 9.94e-16 76.6
MS.gene010021.t1 AT2G24570 46.667 90 44 3 178 267 218 303 1.09e-15 77.4
MS.gene010021.t1 AT2G46130 55.172 58 23 1 205 262 30 84 1.42e-15 72.4
MS.gene010021.t1 AT2G23320 41.176 85 46 3 179 262 214 295 1.73e-15 76.6
MS.gene010021.t1 AT1G69310 49.254 67 30 2 205 271 147 209 2.72e-15 75.9
MS.gene010021.t1 AT1G69310 49.254 67 30 2 205 271 147 209 2.72e-15 75.9
MS.gene010021.t1 AT1G69310 49.254 67 30 2 205 271 147 209 2.72e-15 75.9
MS.gene010021.t1 AT4G26440 48.052 77 27 3 203 279 176 239 3.84e-15 77.0
MS.gene010021.t1 AT5G45260 31.343 134 88 1 133 262 1132 1265 6.65e-15 76.6
MS.gene010021.t1 AT4G18170 27.925 265 158 9 76 338 59 292 8.56e-15 74.7
MS.gene010021.t1 AT5G28650 45.882 85 42 3 179 262 236 317 8.88e-15 74.7
MS.gene010021.t1 AT4G39410 42.857 84 38 2 205 281 223 303 1.20e-14 74.3
MS.gene010021.t1 AT1G66600 36.134 119 55 3 146 264 65 162 1.49e-14 72.8
MS.gene010021.t1 AT2G34830 43.023 86 43 2 181 262 196 279 1.55e-14 74.7
MS.gene010021.t1 AT2G34830 43.023 86 43 2 181 262 187 270 1.56e-14 74.7
MS.gene010021.t1 AT5G22570 49.153 59 30 0 206 264 111 169 1.90e-14 73.6
MS.gene010021.t1 AT3G04670 41.237 97 53 3 179 274 236 329 3.30e-14 73.2
MS.gene010021.t1 AT2G37260 43.925 107 53 3 173 279 321 420 3.35e-14 73.9
MS.gene010021.t1 AT2G37260 54.386 57 22 2 206 262 166 218 1.85e-13 71.6
MS.gene010021.t1 AT3G58710 43.478 92 43 4 176 262 39 126 3.80e-14 72.4
MS.gene010021.t1 AT2G37260 43.925 107 53 3 173 279 239 338 3.95e-14 73.2
MS.gene010021.t1 AT2G37260 54.386 57 22 2 206 262 84 136 1.91e-13 71.2
MS.gene010021.t1 AT2G37260 43.925 107 53 3 173 279 241 340 4.00e-14 73.2
MS.gene010021.t1 AT2G37260 54.386 57 22 2 206 262 86 138 1.88e-13 71.2
MS.gene010021.t1 AT2G44745 53.448 58 24 1 205 262 145 199 4.23e-14 71.2
MS.gene010021.t1 AT1G29860 55.172 58 23 1 205 262 136 190 5.25e-14 72.0
MS.gene010021.t1 AT1G80590 50.847 59 29 0 206 264 86 144 6.76e-14 70.9
MS.gene010021.t1 AT1G30650 43.023 86 43 2 181 262 189 272 6.86e-14 72.8
MS.gene010021.t1 AT2G03340 48.529 68 29 3 196 262 241 303 7.84e-14 72.8
MS.gene010021.t1 AT2G03340 40.964 83 46 1 205 287 415 494 2.57e-11 65.1
MS.gene010021.t1 AT3G01080 48.387 62 28 2 201 262 201 258 8.39e-14 72.0
MS.gene010021.t1 AT3G58710 40.426 94 48 3 174 262 35 125 1.05e-13 70.9
MS.gene010021.t1 AT3G01080 48.387 62 28 2 201 262 201 258 2.69e-13 71.2
MS.gene010021.t1 AT5G46350 51.724 58 25 1 205 262 183 237 2.95e-13 70.5
MS.gene010021.t1 AT5G13080 53.448 58 24 1 205 262 67 121 3.38e-13 67.0
MS.gene010021.t1 AT3G01080 48.387 62 28 2 201 262 163 220 3.97e-13 70.5
MS.gene010021.t1 AT2G38470 43.182 88 40 4 181 262 154 237 4.07e-13 70.9
MS.gene010021.t1 AT1G13960 45.833 72 33 3 192 262 216 282 4.27e-13 70.9
MS.gene010021.t1 AT1G13960 46.552 58 28 1 205 262 409 463 8.73e-11 63.5
MS.gene010021.t1 AT1G13960 45.833 72 33 3 192 262 63 129 7.88e-13 69.3
MS.gene010021.t1 AT4G24240 41.758 91 49 3 173 262 249 336 9.33e-13 68.9
MS.gene010021.t1 AT5G52830 48.780 82 34 3 181 262 147 220 1.01e-12 68.9
MS.gene010021.t1 AT5G26170 46.552 58 28 1 205 262 113 167 1.15e-12 66.2
MS.gene010021.t1 AT4G31800 43.284 67 36 1 196 262 102 166 1.80e-12 67.0
MS.gene010021.t1 AT4G26640 48.276 58 27 1 205 262 341 395 1.90e-12 68.6
MS.gene010021.t1 AT5G56270 45.455 77 29 3 203 279 271 334 1.92e-12 68.9
MS.gene010021.t1 AT5G49520 50.000 58 26 1 205 262 221 275 1.98e-12 68.2
MS.gene010021.t1 AT4G04450 42.045 88 38 3 206 293 199 273 1.99e-12 68.6
MS.gene010021.t1 AT4G26640 48.276 58 27 1 205 262 381 435 2.14e-12 68.6
MS.gene010021.t1 AT4G23550 52.632 57 25 1 206 262 141 195 2.15e-12 67.4
MS.gene010021.t1 AT4G26640 48.276 58 27 1 205 262 205 259 2.18e-12 68.2
MS.gene010021.t1 AT4G26640 48.276 58 27 1 205 262 205 259 2.18e-12 68.2
MS.gene010021.t1 AT4G26640 48.276 58 27 1 205 262 205 259 2.18e-12 68.2
MS.gene010021.t1 AT4G26640 48.276 58 27 1 205 262 205 259 2.18e-12 68.2
MS.gene010021.t1 AT4G26640 48.276 58 27 1 205 262 309 363 2.26e-12 68.6
MS.gene010021.t1 AT4G31800 43.284 67 36 1 196 262 167 231 2.39e-12 67.4
MS.gene010021.t1 AT4G04450 42.045 88 38 3 206 293 293 367 2.93e-12 68.2
MS.gene010021.t1 AT4G30935 42.857 77 40 2 206 282 332 404 3.31e-12 67.8
MS.gene010021.t1 AT2G47260 38.462 91 53 1 180 270 149 236 7.54e-12 66.2
MS.gene010021.t1 AT4G01250 38.554 83 44 2 180 262 108 183 1.10e-11 65.5
MS.gene010021.t1 AT1G62300 52.632 57 25 1 206 262 313 367 1.76e-11 65.9
MS.gene010021.t1 AT4G01720 52.632 57 25 1 206 262 240 294 2.48e-11 65.1
MS.gene010021.t1 AT2G30250 52.632 57 24 1 206 262 329 382 3.04e-11 64.7
MS.gene010021.t1 AT5G43290 49.153 59 27 1 205 263 114 169 3.39e-11 63.5
MS.gene010021.t1 AT5G45050 42.353 85 42 2 178 262 1158 1235 4.02e-11 65.1
MS.gene010021.t1 AT4G22070 52.632 57 25 1 206 262 298 352 4.13e-11 64.7
MS.gene010021.t1 AT4G22070 52.632 57 25 1 206 262 298 352 4.13e-11 64.7
MS.gene010021.t1 AT4G22070 52.632 57 25 1 206 262 298 352 4.13e-11 64.7
MS.gene010021.t1 AT4G22070 52.632 57 25 1 206 262 298 352 4.13e-11 64.7
MS.gene010021.t1 AT5G45050 42.353 85 42 2 178 262 1130 1207 4.15e-11 65.1
MS.gene010021.t1 AT1G55600 44.776 67 34 1 196 262 298 361 4.22e-11 64.7
MS.gene010021.t1 AT5G45050 42.353 85 42 2 178 262 1019 1096 4.46e-11 65.1
MS.gene010021.t1 AT2G04880 47.368 57 27 1 206 262 284 337 4.73e-11 64.3
MS.gene010021.t1 AT2G04880 51.724 58 24 2 205 262 111 164 9.21e-11 63.5
MS.gene010021.t1 AT2G04880 47.368 57 27 1 206 262 308 361 4.81e-11 64.3
MS.gene010021.t1 AT2G04880 51.724 58 24 2 205 262 111 164 8.24e-11 63.5
MS.gene010021.t1 AT2G25000 37.500 80 48 1 183 262 81 158 5.56e-11 62.4
MS.gene010021.t1 AT2G25000 37.500 80 48 1 183 262 81 158 5.56e-11 62.4
MS.gene010021.t1 AT2G25000 37.500 80 48 1 183 262 103 180 9.07e-11 62.0

Find 95 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATGAGAATAGCATTTC+AGG 0.221599 5.3:-53971037 None:intergenic
AAGTCTTTGCACTTGCTTCT+TGG 0.236235 5.3:-53973730 None:intergenic
TATGTTACTTCAACTATGTT+AGG 0.240903 5.3:-53973762 None:intergenic
CATCATCATTACTCTTTCTT+TGG 0.259697 5.3:-53971089 None:intergenic
GATAGATCAAAAGGGTTAGA+AGG 0.266264 5.3:+53971418 MS.gene010021:CDS
GGCTTTACTTGGAGAAAATA+TGG 0.298786 5.3:+53973026 MS.gene010021:CDS
TGAAATGGGTGGGAGGGATA+TGG 0.320333 5.3:+53971387 MS.gene010021:CDS
TGATTATCCGGTGGCCGATA+TGG 0.327197 5.3:+53973971 MS.gene010021:CDS
AACTTTGAGGATTGGTGAAA+TGG 0.332908 5.3:+53971372 MS.gene010021:CDS
AAAACTCAACATGCTCAAAT+TGG 0.341836 5.3:+53971199 MS.gene010021:CDS
TCACCGCAGGTGGCCACCCT+TGG 0.347403 5.3:-53973890 None:intergenic
CTTTGCACTTGCTTCTTGGC+TGG 0.354716 5.3:-53973726 None:intergenic
TTCAGGGTGGTTGGCCATGT+TGG 0.368022 5.3:-53971020 None:intergenic
CCTTTATCAGAAGTTACTAT+AGG 0.370143 5.3:+53973676 MS.gene010021:intron
TAGTAGCAGTGGCAATAGTA+TGG 0.375087 5.3:+53974016 MS.gene010021:CDS
CTCCAAGTAAAGCCATCTTC+TGG 0.382520 5.3:-53973017 None:intergenic
ATTAGAACACTCACATCAAA+AGG 0.383819 5.3:-53971286 None:intergenic
ACGTTCAGTTGTTAAGGTCT+AGG 0.384626 5.3:+53971347 MS.gene010021:CDS
ATTTGAGCAAAGGAATTAGA+AGG 0.385061 5.3:-53971151 None:intergenic
GAAGGTTTAGATAGATCAAA+AGG 0.386309 5.3:+53971409 MS.gene010021:CDS
TTCAATGCATGCACATTCAA+TGG 0.387023 5.3:+53971240 MS.gene010021:CDS
TAGCAGCAGGAAGAATGATT+TGG 0.389860 5.3:+53972941 MS.gene010021:intron
CACCAGAAGATGGCTTTACT+TGG 0.405190 5.3:+53973015 MS.gene010021:CDS
TCAGGGTGGTTGGCCATGTT+GGG 0.411278 5.3:-53971019 None:intergenic
AAGATTCGGCATTGATTATC+CGG 0.412157 5.3:+53973959 MS.gene010021:CDS
CTGCGGTGAACCACGACCTC+AGG 0.426028 5.3:+53973904 MS.gene010021:CDS
GTGTTTCCAATCTGAGGTGC+TGG 0.434455 5.3:-53972984 None:intergenic
GGAAGAAATGGCAGTTTCAA+TGG 0.437250 5.3:-53973868 None:intergenic
ATGTTACTTCAACTATGTTA+GGG 0.440557 5.3:-53973761 None:intergenic
CATGCTCAAATTGGTGGAAG+TGG 0.441412 5.3:+53971208 MS.gene010021:CDS
CACCGCAGGTGGCCACCCTT+GGG 0.445492 5.3:-53973889 None:intergenic
CTAATGTTCACAAGTAGGTT+TGG 0.447789 5.3:-53973843 None:intergenic
GAACATTACATCGGCCATAT+CGG 0.452060 5.3:-53973985 None:intergenic
ACAAGTAGGTTTGGTGGTGG+TGG 0.452659 5.3:-53973834 None:intergenic
ACTTTGAGGATTGGTGAAAT+GGG 0.454574 5.3:+53971373 MS.gene010021:CDS
ATATGATGATGGTGCTGTTA+AGG 0.457432 5.3:-53973811 None:intergenic
TGGCCACCCTTGGGAAGAAA+TGG 0.460403 5.3:-53973880 None:intergenic
GAAATTCATGACTTCTTCCA+TGG 0.461625 5.3:-53970951 None:intergenic
GGTGGTGGTGGATATGATGA+TGG 0.483740 5.3:-53973822 None:intergenic
TAAGGTCTAGGGAAACTTTG+AGG 0.489231 5.3:+53971359 MS.gene010021:CDS
CTGAGGTCGTGGTTCACCGC+AGG 0.489237 5.3:-53973903 None:intergenic
CGTTCAGTTGTTAAGGTCTA+GGG 0.489911 5.3:+53971348 MS.gene010021:CDS
GGACCCTCTACTTCAAGATT+CGG 0.492644 5.3:+53973945 MS.gene010021:CDS
CGTCTCCGCCCCTGAGGTCG+TGG 0.495381 5.3:-53973914 None:intergenic
CTCAGGGGCGGAGACGGTAC+TGG 0.503754 5.3:+53973921 MS.gene010021:CDS
TCTAGGGAAACTTTGAGGAT+TGG 0.504126 5.3:+53971364 MS.gene010021:CDS
ATGCATCCAGCACCTCAGAT+TGG 0.509798 5.3:+53972978 MS.gene010021:CDS
GCGGTGACGTTCAGTTGTTA+AGG 0.512945 5.3:+53971341 MS.gene010021:CDS
CACGACCTCAGGGGCGGAGA+CGG 0.513045 5.3:+53973915 MS.gene010021:CDS
TTCCCAAGGGTGGCCACCTG+CGG 0.515927 5.3:+53973887 MS.gene010021:CDS
TGGATGAGAATAGCATTTCA+GGG 0.524108 5.3:-53971036 None:intergenic
ATAGTTGAAGTAACATATAG+AGG 0.529154 5.3:+53973768 MS.gene010021:CDS
ATCTCAGTGTTTCCAATCTG+AGG 0.531039 5.3:-53972990 None:intergenic
ACTCAACATGCTCAAATTGG+TGG 0.531204 5.3:+53971202 MS.gene010021:CDS
ACTGAGATGCCACCAGAAGA+TGG 0.534882 5.3:+53973005 MS.gene010021:CDS
GAATAGCATTTCAGGGTGGT+TGG 0.535922 5.3:-53971029 None:intergenic
ATGATAAGTGGCGGTGGTGA+TGG 0.538282 5.3:+53971319 MS.gene010021:CDS
AAGGTTTAGATAGATCAAAA+GGG 0.540645 5.3:+53971410 MS.gene010021:CDS
ATTGAGAATAAGATGATAAG+TGG 0.540967 5.3:+53971307 MS.gene010021:CDS
GAAACTGCCATTTCTTCCCA+AGG 0.545394 5.3:+53973873 MS.gene010021:CDS
TGGTACTAATGTTCACAAGT+AGG 0.547624 5.3:-53973848 None:intergenic
TGCGGTGAACCACGACCTCA+GGG 0.552440 5.3:+53973905 MS.gene010021:CDS
AGGTCGTGGTTCACCGCAGG+TGG 0.554696 5.3:-53973900 None:intergenic
TACTTGGCCGCAAGCACCCA+AGG 0.555851 5.3:+53973060 MS.gene010021:CDS
ATGTTCACAAGTAGGTTTGG+TGG 0.557977 5.3:-53973840 None:intergenic
TAATTTACCTTGGGTGCTTG+CGG 0.559902 5.3:-53973067 None:intergenic
TTGAGGATTGGTGAAATGGG+TGG 0.562645 5.3:+53971376 MS.gene010021:CDS
AGGGGCGGAGACGGTACTGG+TGG 0.562847 5.3:+53973924 MS.gene010021:CDS
TGAGGATTGGTGAAATGGGT+GGG 0.564470 5.3:+53971377 MS.gene010021:CDS
GGCCGATGTAATGTTCAACT+CGG 0.582893 5.3:+53973992 MS.gene010021:CDS
AAACTGCCATTTCTTCCCAA+GGG 0.586934 5.3:+53973874 MS.gene010021:CDS
GGATTGGTGAAATGGGTGGG+AGG 0.593788 5.3:+53971380 MS.gene010021:CDS
ATGCCGAATCTTGAAGTAGA+GGG 0.595101 5.3:-53973948 None:intergenic
TTAGAACACTCACATCAAAA+GGG 0.595617 5.3:-53971285 None:intergenic
CTGCCATTTCTTCCCAAGGG+TGG 0.604947 5.3:+53973877 MS.gene010021:CDS
TTCAACTCGGGTAGTAGCAG+TGG 0.607187 5.3:+53974005 MS.gene010021:CDS
GATTGGTGAAATGGGTGGGA+GGG 0.607995 5.3:+53971381 MS.gene010021:CDS
ATGAGACAGAATTTGAGCAA+AGG 0.616039 5.3:-53971161 None:intergenic
AGATAAAGGTGAACCTGATG+AGG 0.619424 5.3:+53974061 MS.gene010021:CDS
TTCACAAGTAGGTTTGGTGG+TGG 0.620288 5.3:-53973837 None:intergenic
GCCGATGTAATGTTCAACTC+GGG 0.623899 5.3:+53973993 MS.gene010021:CDS
AATGCCGAATCTTGAAGTAG+AGG 0.627530 5.3:-53973949 None:intergenic
CAAGTAAAGCCATCTTCTGG+TGG 0.629125 5.3:-53973014 None:intergenic
GAGAATAAGATGATAAGTGG+CGG 0.650628 5.3:+53971310 MS.gene010021:CDS
AATAAGATGATAAGTGGCGG+TGG 0.651861 5.3:+53971313 MS.gene010021:CDS
ACATCGGCCATATCGGCCAC+CGG 0.656162 5.3:-53973978 None:intergenic
GTGAACCACGACCTCAGGGG+CGG 0.657610 5.3:+53973909 MS.gene010021:CDS
ATAAGTGGCGGTGGTGATGG+CGG 0.661391 5.3:+53971322 MS.gene010021:CDS
TGAACTAGAACTACCCAACA+TGG 0.663075 5.3:+53971006 MS.gene010021:CDS
ATTCGGCATTGATTATCCGG+TGG 0.666772 5.3:+53973962 MS.gene010021:CDS
ATGGGTGGGAGGGATATGGA+AGG 0.678067 5.3:+53971391 MS.gene010021:CDS
CAGTACCGTCTCCGCCCCTG+AGG 0.681574 5.3:-53973920 None:intergenic
ACCCGAGTTGAACATTACAT+CGG 0.690614 5.3:-53973994 None:intergenic
GCGGTGAACCACGACCTCAG+GGG 0.741552 5.3:+53973906 MS.gene010021:CDS
ATGAGAATAGCATTTCAGGG+TGG 0.763743 5.3:-53971033 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTGAATTTTTTTTTTT+TGG - chr5.3:53972753-53972772 None:intergenic 10.0%
!!! AAAATCAATTTTTTGTTTTA+TGG - chr5.3:53972475-53972494 None:intergenic 10.0%
!!! GTTTTTATTATATAATTTGT+TGG + chr5.3:53973633-53973652 MS.gene010021:intron 10.0%
!!! TTTTACATAAAATTATAAGT+AGG + chr5.3:53972842-53972861 MS.gene010021:intron 10.0%
!! AAATATTTAACAAGTAATCA+AGG - chr5.3:53973426-53973445 None:intergenic 15.0%
!! AATTATTGTTAATTTACCTT+GGG - chr5.3:53973079-53973098 None:intergenic 15.0%
!! ACATTATAAAACTTTAAATG+AGG + chr5.3:53972042-53972061 MS.gene010021:intron 15.0%
!! AGAATTGTACTTTATAATAT+AGG + chr5.3:53973103-53973122 MS.gene010021:intron 15.0%
!! GAATTGTACTTTATAATATA+GGG + chr5.3:53973104-53973123 MS.gene010021:intron 15.0%
!! TAATTATTGTTAATTTACCT+TGG - chr5.3:53973080-53973099 None:intergenic 15.0%
!! TCAAAGTTTCATATAATAAA+TGG + chr5.3:53971563-53971582 MS.gene010021:intron 15.0%
!!! TCACTAGTTAATTATTTTAA+TGG + chr5.3:53972649-53972668 MS.gene010021:intron 15.0%
!!! TGTTTATTTTTTAAACATCT+TGG + chr5.3:53973611-53973630 MS.gene010021:intron 15.0%
!!! TTATATACATACCATTTTTT+TGG - chr5.3:53971481-53971500 None:intergenic 15.0%
!! AAATTTATATATGCAAGATC+CGG + chr5.3:53972700-53972719 MS.gene010021:intron 20.0%
!! AAGAAATATTCTTAGAAGAA+AGG - chr5.3:53973212-53973231 None:intergenic 20.0%
!! AATAGTTTGACACAATTATT+AGG + chr5.3:53972779-53972798 MS.gene010021:intron 20.0%
!! CAATTATTAGGAAGTTAATA+TGG + chr5.3:53972791-53972810 MS.gene010021:intron 20.0%
!! TTTCTTTCAATTATTGTTGT+TGG - chr5.3:53972201-53972220 None:intergenic 20.0%
!!! AGTGAATTTTTTTTTTTTGG+TGG - chr5.3:53972750-53972769 None:intergenic 20.0%
!!! ATTTCTAAGGTATTGAATTT+AGG + chr5.3:53971672-53971691 MS.gene010021:intron 20.0%
!!! TTTCTTCTAAGAATATTTCT+TGG + chr5.3:53973211-53973230 MS.gene010021:intron 20.0%
!!! TTTTATCTAACTGAAGATAA+AGG + chr5.3:53974047-53974066 MS.gene010021:CDS 20.0%
!!! TTTTTTTGTGTTTGCTATTT+TGG + chr5.3:53972509-53972528 MS.gene010021:intron 20.0%
! AACTTTAAATGAGGTTAACT+TGG + chr5.3:53972051-53972070 MS.gene010021:intron 25.0%
! AAGGTTTAGATAGATCAAAA+GGG + chr5.3:53971410-53971429 MS.gene010021:CDS 25.0%
! AATTTATATATGCAAGATCC+GGG + chr5.3:53972701-53972720 MS.gene010021:intron 25.0%
! ATAATGTGAAAATCCTAAGT+AGG - chr5.3:53972029-53972048 None:intergenic 25.0%
! ATACATCAAAGTTGAAAGTT+AGG - chr5.3:53971914-53971933 None:intergenic 25.0%
! ATAGTTGAAGTAACATATAG+AGG + chr5.3:53973768-53973787 MS.gene010021:CDS 25.0%
! ATGTTATTGAATCAAAGTCT+TGG + chr5.3:53971609-53971628 MS.gene010021:intron 25.0%
! ATTCAATAACATAGATTGTG+AGG - chr5.3:53971601-53971620 None:intergenic 25.0%
! ATTGAGAATAAGATGATAAG+TGG + chr5.3:53971307-53971326 MS.gene010021:CDS 25.0%
! GTATAATTCAGTTGTTAGAT+TGG + chr5.3:53972813-53972832 MS.gene010021:intron 25.0%
! TAATGTGAAAATCCTAAGTA+GGG - chr5.3:53972028-53972047 None:intergenic 25.0%
! TGAAACTTTCAAACTCTTTA+TGG - chr5.3:53973139-53973158 None:intergenic 25.0%
! TTGCAATTCAACTGAAATTA+AGG - chr5.3:53971842-53971861 None:intergenic 25.0%
!! ATGTAAGATAGAATTTTCCA+TGG + chr5.3:53972321-53972340 MS.gene010021:intron 25.0%
!! ATGTTACTTCAACTATGTTA+GGG - chr5.3:53973764-53973783 None:intergenic 25.0%
!! ATTCTTAGAAGAAAGGTATA+AGG - chr5.3:53973205-53973224 None:intergenic 25.0%
!! CTGGACACAAATATAATTTT+TGG - chr5.3:53973710-53973729 None:intergenic 25.0%
!! TAGGGTGAAAATTTTCATTA+TGG - chr5.3:53972010-53972029 None:intergenic 25.0%
!! TATGTTACTTCAACTATGTT+AGG - chr5.3:53973765-53973784 None:intergenic 25.0%
!!! CAACACAAAGATTATTTCTA+AGG + chr5.3:53971659-53971678 MS.gene010021:intron 25.0%
!!! CATATTTGGGTTTTAGTTTA+TGG + chr5.3:53971752-53971771 MS.gene010021:intron 25.0%
!!! GAATTTTTTTTTTTTGGTGG+TGG - chr5.3:53972747-53972766 None:intergenic 25.0%
AAAACTCAACATGCTCAAAT+TGG + chr5.3:53971199-53971218 MS.gene010021:CDS 30.0%
AACACACCAATAATAACGTA+CGG - chr5.3:53972274-53972293 None:intergenic 30.0%
ACCCAAATATGTAAATTACC+CGG - chr5.3:53971743-53971762 None:intergenic 30.0%
ACCGGGTAATTTACATATTT+GGG + chr5.3:53971739-53971758 MS.gene010021:intron 30.0%
ATGTGTGTATGATTGAATCT+AGG + chr5.3:53972081-53972100 MS.gene010021:intron 30.0%
ATTAGAACACTCACATCAAA+AGG - chr5.3:53971289-53971308 None:intergenic 30.0%
ATTTATATATGCAAGATCCG+GGG + chr5.3:53972702-53972721 MS.gene010021:intron 30.0%
ATTTGAGCAAAGGAATTAGA+AGG - chr5.3:53971154-53971173 None:intergenic 30.0%
CATCATCATTACTCTTTCTT+TGG - chr5.3:53971092-53971111 None:intergenic 30.0%
CATGGTCTTTCTTAGTAATT+AGG + chr5.3:53972339-53972358 MS.gene010021:intron 30.0%
CCTATAGTAACTTCTGATAA+AGG - chr5.3:53973679-53973698 None:intergenic 30.0%
CCTTTATCAGAAGTTACTAT+AGG + chr5.3:53973676-53973695 MS.gene010021:intron 30.0%
CTAATTACTAAGAAAGACCA+TGG - chr5.3:53972341-53972360 None:intergenic 30.0%
GAAGGTTTAGATAGATCAAA+AGG + chr5.3:53971409-53971428 MS.gene010021:CDS 30.0%
TAGTTTACATGACCAAAACA+AGG + chr5.3:53972412-53972431 MS.gene010021:intron 30.0%
TATGGCCAAAAAGAAATACT+TGG + chr5.3:53973044-53973063 MS.gene010021:CDS 30.0%
TCTACAATTGTGACAAAAAC+AGG - chr5.3:53972160-53972179 None:intergenic 30.0%
TGTGAATGAGTCAATTCATA+GGG - chr5.3:53973272-53973291 None:intergenic 30.0%
TTAGAACACTCACATCAAAA+GGG - chr5.3:53971288-53971307 None:intergenic 30.0%
! ACAAAAGTGAAATGCTTTTG+AGG + chr5.3:53972225-53972244 MS.gene010021:intron 30.0%
! CTTTCAATTATTGTTGTTGG+CGG - chr5.3:53972198-53972217 None:intergenic 30.0%
! GGTATTGAAAGCTTTAGTTT+AGG + chr5.3:53972246-53972265 MS.gene010021:intron 30.0%
! TCTGTAAGATTTTCACTAGA+GGG + chr5.3:53971981-53972000 MS.gene010021:intron 30.0%
! TGAAAATTTTCACCCTACTT+AGG + chr5.3:53972013-53972032 MS.gene010021:intron 30.0%
! TTCTGTAAGATTTTCACTAG+AGG + chr5.3:53971980-53971999 MS.gene010021:intron 30.0%
!! AAACAAGGTCTTTGCTATTT+AGG + chr5.3:53972427-53972446 MS.gene010021:intron 30.0%
!! AAATAGCAAAGACCTTGTTT+TGG - chr5.3:53972427-53972446 None:intergenic 30.0%
!! AAGAAAGGTATAAGGTTTTC+TGG - chr5.3:53973197-53973216 None:intergenic 30.0%
!!! GGGTTTTAGTTTATGGTTAT+AGG + chr5.3:53971759-53971778 MS.gene010021:intron 30.0%
!!! TACCATTTTTTTGGTCTTGA+AGG - chr5.3:53971472-53971491 None:intergenic 30.0%
!!! TGCTTTTTCTACATCATCAA+TGG - chr5.3:53971059-53971078 None:intergenic 30.0%
AATTAGGCAACAAGTCATCA+TGG + chr5.3:53972355-53972374 MS.gene010021:intron 35.0%
ATATGTAAATTACCCGGTGT+TGG - chr5.3:53971737-53971756 None:intergenic 35.0%
ATGAGACAGAATTTGAGCAA+AGG - chr5.3:53971164-53971183 None:intergenic 35.0%
ATGGATGAGAATAGCATTTC+AGG - chr5.3:53971040-53971059 None:intergenic 35.0%
CACCGGGTAATTTACATATT+TGG + chr5.3:53971738-53971757 MS.gene010021:intron 35.0%
CACCTTCAAGACCAAAAAAA+TGG + chr5.3:53971467-53971486 MS.gene010021:CDS 35.0%
CTAATGTTCACAAGTAGGTT+TGG - chr5.3:53973846-53973865 None:intergenic 35.0%
GAGAATAAGATGATAAGTGG+CGG + chr5.3:53971310-53971329 MS.gene010021:CDS 35.0%
GATAGATCAAAAGGGTTAGA+AGG + chr5.3:53971418-53971437 MS.gene010021:CDS 35.0%
GGCTTTACTTGGAGAAAATA+TGG + chr5.3:53973026-53973045 MS.gene010021:CDS 35.0%
GTGTCTAAGAACTGTGTATT+TGG + chr5.3:53972535-53972554 MS.gene010021:intron 35.0%
GTGTGAATGAGTCAATTCAT+AGG - chr5.3:53973273-53973292 None:intergenic 35.0%
TAGGATCCGTACGTTATTAT+TGG + chr5.3:53972265-53972284 MS.gene010021:intron 35.0%
TCAATCATACACACATTGTG+AGG - chr5.3:53972077-53972096 None:intergenic 35.0%
TCACACACAAACACACATAT+AGG - chr5.3:53972883-53972902 None:intergenic 35.0%
TGGATGAGAATAGCATTTCA+GGG - chr5.3:53971039-53971058 None:intergenic 35.0%
TGGTACTAATGTTCACAAGT+AGG - chr5.3:53973851-53973870 None:intergenic 35.0%
TGGTGTGTTTGTAAACAGAA+AGG + chr5.3:53972285-53972304 MS.gene010021:intron 35.0%
TTATAGGTGCCTTGCTAATT+GGG + chr5.3:53971775-53971794 MS.gene010021:intron 35.0%
TTCAATGCATGCACATTCAA+TGG + chr5.3:53971240-53971259 MS.gene010021:CDS 35.0%
! AACTTTGAGGATTGGTGAAA+TGG + chr5.3:53971372-53971391 MS.gene010021:CDS 35.0%
! AAGGTATAAGGTTTTCTGGA+TGG - chr5.3:53973193-53973212 None:intergenic 35.0%
! ACTTTGAGGATTGGTGAAAT+GGG + chr5.3:53971373-53971392 MS.gene010021:CDS 35.0%
! ATGTGAAGGCACAACAAATT+AGG - chr5.3:53972914-53972933 None:intergenic 35.0%
! GGGTCACTAGTTTATGAAAA+TGG + chr5.3:53972117-53972136 MS.gene010021:intron 35.0%
! GGTATTGAATTTAGGTGTTG+AGG + chr5.3:53971680-53971699 MS.gene010021:intron 35.0%
! TTTCAGTGTTTTTCCAACAC+CGG + chr5.3:53971721-53971740 MS.gene010021:intron 35.0%
!! AAGATTCGGCATTGATTATC+CGG + chr5.3:53973959-53973978 MS.gene010021:CDS 35.0%
!! ATATGATGATGGTGCTGTTA+AGG - chr5.3:53973814-53973833 None:intergenic 35.0%
!!! CATTTTTTTGGTCTTGAAGG+TGG - chr5.3:53971469-53971488 None:intergenic 35.0%
!!! TTTTTTTTTTTGGTGGTGGC+CGG - chr5.3:53972743-53972762 None:intergenic 35.0%
AACTAGTGACCCAATTAGCA+AGG - chr5.3:53972109-53972128 None:intergenic 40.0%
AAGTCTTTGCACTTGCTTCT+TGG - chr5.3:53973733-53973752 None:intergenic 40.0%
AATAAGATGATAAGTGGCGG+TGG + chr5.3:53971313-53971332 MS.gene010021:CDS 40.0%
AATCTAGGTGCCTTGCTAAT+TGG + chr5.3:53972096-53972115 MS.gene010021:intron 40.0%
AATGCCGAATCTTGAAGTAG+AGG - chr5.3:53973952-53973971 None:intergenic 40.0%
ACGTTCAGTTGTTAAGGTCT+AGG + chr5.3:53971347-53971366 MS.gene010021:CDS 40.0%
ACTCAACATGCTCAAATTGG+TGG + chr5.3:53971202-53971221 MS.gene010021:CDS 40.0%
AGATAAAGGTGAACCTGATG+AGG + chr5.3:53974061-53974080 MS.gene010021:CDS 40.0%
ATCTAGGTGCCTTGCTAATT+GGG + chr5.3:53972097-53972116 MS.gene010021:intron 40.0%
ATCTAGTTGCCCAATTAGCA+AGG - chr5.3:53971787-53971806 None:intergenic 40.0%
ATCTCAGTGTTTCCAATCTG+AGG - chr5.3:53972993-53973012 None:intergenic 40.0%
ATGAGAATAGCATTTCAGGG+TGG - chr5.3:53971036-53971055 None:intergenic 40.0%
ATGCCGAATCTTGAAGTAGA+GGG - chr5.3:53973951-53973970 None:intergenic 40.0%
ATGTTCACAAGTAGGTTTGG+TGG - chr5.3:53973843-53973862 None:intergenic 40.0%
CAAAGTTGAAAGTTAGGTCG+TGG - chr5.3:53971908-53971927 None:intergenic 40.0%
CGTTCAGTTGTTAAGGTCTA+GGG + chr5.3:53971348-53971367 MS.gene010021:CDS 40.0%
GAACATTACATCGGCCATAT+CGG - chr5.3:53973988-53974007 None:intergenic 40.0%
GGAAGAAATGGCAGTTTCAA+TGG - chr5.3:53973871-53973890 None:intergenic 40.0%
GTTATAGGTGCCTTGCTAAT+TGG + chr5.3:53971774-53971793 MS.gene010021:intron 40.0%
TAAGGTCTAGGGAAACTTTG+AGG + chr5.3:53971359-53971378 MS.gene010021:CDS 40.0%
TAATTTACCTTGGGTGCTTG+CGG - chr5.3:53973070-53973089 None:intergenic 40.0%
TAGCAGCAGGAAGAATGATT+TGG + chr5.3:53972941-53972960 MS.gene010021:intron 40.0%
TAGTAGCAGTGGCAATAGTA+TGG + chr5.3:53974016-53974035 MS.gene010021:CDS 40.0%
TGAACTAGAACTACCCAACA+TGG + chr5.3:53971006-53971025 MS.gene010021:CDS 40.0%
! AAACTGCCATTTCTTCCCAA+GGG + chr5.3:53973874-53973893 MS.gene010021:CDS 40.0%
! ACCCGAGTTGAACATTACAT+CGG - chr5.3:53973997-53974016 None:intergenic 40.0%
! TCTAGGGAAACTTTGAGGAT+TGG + chr5.3:53971364-53971383 MS.gene010021:CDS 40.0%
! TGCGGCCAAGTATTTCTTTT+TGG - chr5.3:53973052-53973071 None:intergenic 40.0%
! TTCAGTGTTTTTCCAACACC+GGG + chr5.3:53971722-53971741 MS.gene010021:intron 40.0%
!!! TTTTTTTTTTGGTGGTGGCC+GGG - chr5.3:53972742-53972761 None:intergenic 40.0%
CAAGTAAAGCCATCTTCTGG+TGG - chr5.3:53973017-53973036 None:intergenic 45.0%
CATGCTCAAATTGGTGGAAG+TGG + chr5.3:53971208-53971227 MS.gene010021:CDS 45.0%
CTCCAAGTAAAGCCATCTTC+TGG - chr5.3:53973020-53973039 None:intergenic 45.0%
GAATAGCATTTCAGGGTGGT+TGG - chr5.3:53971032-53971051 None:intergenic 45.0%
GCCGATGTAATGTTCAACTC+GGG + chr5.3:53973993-53974012 MS.gene010021:CDS 45.0%
GCTGTCTTGTGTGAATGATG+AGG - chr5.3:53972387-53972406 None:intergenic 45.0%
GGACCCTCTACTTCAAGATT+CGG + chr5.3:53973945-53973964 MS.gene010021:CDS 45.0%
GGCCGATGTAATGTTCAACT+CGG + chr5.3:53973992-53974011 MS.gene010021:CDS 45.0%
TGTTGTGCCTTCACATGCAT+TGG + chr5.3:53972918-53972937 MS.gene010021:intron 45.0%
! CACCAGAAGATGGCTTTACT+TGG + chr5.3:53973015-53973034 MS.gene010021:CDS 45.0%
! GAAACTGCCATTTCTTCCCA+AGG + chr5.3:53973873-53973892 MS.gene010021:CDS 45.0%
! TGAGGATTGGTGAAATGGGT+GGG + chr5.3:53971377-53971396 MS.gene010021:CDS 45.0%
! TTGAGGATTGGTGAAATGGG+TGG + chr5.3:53971376-53971395 MS.gene010021:CDS 45.0%
!! ATTCGGCATTGATTATCCGG+TGG + chr5.3:53973962-53973981 MS.gene010021:CDS 45.0%
!! TTCACAAGTAGGTTTGGTGG+TGG - chr5.3:53973840-53973859 None:intergenic 45.0%
!!! TTTTTTTTTGGTGGTGGCCG+GGG - chr5.3:53972741-53972760 None:intergenic 45.0%
ACTGAGATGCCACCAGAAGA+TGG + chr5.3:53973005-53973024 MS.gene010021:CDS 50.0%
ATGATAAGTGGCGGTGGTGA+TGG + chr5.3:53971319-53971338 MS.gene010021:CDS 50.0%
ATGCATCCAGCACCTCAGAT+TGG + chr5.3:53972978-53972997 MS.gene010021:CDS 50.0%
CTTTGCACTTGCTTCTTGGC+TGG - chr5.3:53973729-53973748 None:intergenic 50.0%
GATTGGTGAAATGGGTGGGA+GGG + chr5.3:53971381-53971400 MS.gene010021:CDS 50.0%
GCGGTGACGTTCAGTTGTTA+AGG + chr5.3:53971341-53971360 MS.gene010021:CDS 50.0%
GCTGCTACCAATGCATGTGA+AGG - chr5.3:53972928-53972947 None:intergenic 50.0%
TGAAATGGGTGGGAGGGATA+TGG + chr5.3:53971387-53971406 MS.gene010021:CDS 50.0%
TGATTATCCGGTGGCCGATA+TGG + chr5.3:53973971-53973990 MS.gene010021:CDS 50.0%
TTCAACTCGGGTAGTAGCAG+TGG + chr5.3:53974005-53974024 MS.gene010021:CDS 50.0%
! GTGTTTCCAATCTGAGGTGC+TGG - chr5.3:53972987-53973006 None:intergenic 50.0%
! TCACATGCATTGGTAGCAGC+AGG + chr5.3:53972928-53972947 MS.gene010021:intron 50.0%
!! ACAAGTAGGTTTGGTGGTGG+TGG - chr5.3:53973837-53973856 None:intergenic 50.0%
!! GGTGGTGGTGGATATGATGA+TGG - chr5.3:53973825-53973844 None:intergenic 50.0%
ATGGGTGGGAGGGATATGGA+AGG + chr5.3:53971391-53971410 MS.gene010021:CDS 55.0%
TGGCCACCCTTGGGAAGAAA+TGG - chr5.3:53973883-53973902 None:intergenic 55.0%
! CTGCCATTTCTTCCCAAGGG+TGG + chr5.3:53973877-53973896 MS.gene010021:CDS 55.0%
! GGATTGGTGAAATGGGTGGG+AGG + chr5.3:53971380-53971399 MS.gene010021:CDS 55.0%
! TCAGGGTGGTTGGCCATGTT+GGG - chr5.3:53971022-53971041 None:intergenic 55.0%
! TTCAGGGTGGTTGGCCATGT+TGG - chr5.3:53971023-53971042 None:intergenic 55.0%
!! ATAAGTGGCGGTGGTGATGG+CGG + chr5.3:53971322-53971341 MS.gene010021:CDS 55.0%
ACATCGGCCATATCGGCCAC+CGG - chr5.3:53973981-53974000 None:intergenic 60.0%
GGGGTTCAAACCTCGAACCC+CGG + chr5.3:53972721-53972740 MS.gene010021:intron 60.0%
GGGGTTCGAGGTTTGAACCC+CGG - chr5.3:53972722-53972741 None:intergenic 60.0%
TACTTGGCCGCAAGCACCCA+AGG + chr5.3:53973060-53973079 MS.gene010021:CDS 60.0%
TGCGGTGAACCACGACCTCA+GGG + chr5.3:53973905-53973924 MS.gene010021:CDS 60.0%
AGGTCGTGGTTCACCGCAGG+TGG - chr5.3:53973903-53973922 None:intergenic 65.0%
CTGAGGTCGTGGTTCACCGC+AGG - chr5.3:53973906-53973925 None:intergenic 65.0%
CTGCGGTGAACCACGACCTC+AGG + chr5.3:53973904-53973923 MS.gene010021:CDS 65.0%
GCGGTGAACCACGACCTCAG+GGG + chr5.3:53973906-53973925 MS.gene010021:CDS 65.0%
GTGAACCACGACCTCAGGGG+CGG + chr5.3:53973909-53973928 MS.gene010021:CDS 65.0%
TTCCCAAGGGTGGCCACCTG+CGG + chr5.3:53973887-53973906 MS.gene010021:CDS 65.0%
CACCGCAGGTGGCCACCCTT+GGG - chr5.3:53973892-53973911 None:intergenic 70.0%
CACGACCTCAGGGGCGGAGA+CGG + chr5.3:53973915-53973934 MS.gene010021:CDS 70.0%
CAGTACCGTCTCCGCCCCTG+AGG - chr5.3:53973923-53973942 None:intergenic 70.0%
CTCAGGGGCGGAGACGGTAC+TGG + chr5.3:53973921-53973940 MS.gene010021:CDS 70.0%
TCACCGCAGGTGGCCACCCT+TGG - chr5.3:53973893-53973912 None:intergenic 70.0%
! AGGGGCGGAGACGGTACTGG+TGG + chr5.3:53973924-53973943 MS.gene010021:CDS 70.0%
!! TTGGTGGTGGCCGGGGTTCG+AGG - chr5.3:53972734-53972753 None:intergenic 70.0%
CGTCTCCGCCCCTGAGGTCG+TGG - chr5.3:53973917-53973936 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 53970953 53974094 53970953 ID=MS.gene010021
chr5.3 mRNA 53970953 53974094 53970953 ID=MS.gene010021.t1;Parent=MS.gene010021
chr5.3 exon 53970953 53971488 53970953 ID=MS.gene010021.t1.exon1;Parent=MS.gene010021.t1
chr5.3 CDS 53970953 53971488 53970953 ID=cds.MS.gene010021.t1;Parent=MS.gene010021.t1
chr5.3 exon 53972944 53973081 53972944 ID=MS.gene010021.t1.exon2;Parent=MS.gene010021.t1
chr5.3 CDS 53972944 53973081 53972944 ID=cds.MS.gene010021.t1;Parent=MS.gene010021.t1
chr5.3 exon 53973686 53974094 53973686 ID=MS.gene010021.t1.exon3;Parent=MS.gene010021.t1
chr5.3 CDS 53973686 53974094 53973686 ID=cds.MS.gene010021.t1;Parent=MS.gene010021.t1
Gene Sequence

>MS.gene010021

ATGGAAGAAGTCATGAATTTCATCATGCGTGCTTTTGAATTAGCAAAAAATCTTGAACTAGAACTACCCAACATGGCCAACCACCCTGAAATGCTATTCTCATCCATTGATGATGTAGAAAAAGCATTTAATGCTGCCAAAGAAAGAGTAATGATGATGTTGTCATCACAACATGACACAACAACAACAACAACAAAACCTTCTAATTCCTTTGCTCAAATTCTGTCTCATGATGTTTTCATGCAAAAAACTCAACATGCTCAAATTGGTGGAAGTGGAAGTGCAATTTCAATGCATGCACATTCAATGGATCAATTGCTTCTAATGCAACACCCTTTTGATGTGAGTGTTCTAATTGAGAATAAGATGATAAGTGGCGGTGGTGATGGCGGTGACGTTCAGTTGTTAAGGTCTAGGGAAACTTTGAGGATTGGTGAAATGGGTGGGAGGGATATGGAAGGTTTAGATAGATCAAAAGGGTTAGAAGGAGATCAAATGCAAGCAATAGAAACACCACCTTCAAGACCAAAAAAATGGTATGTATATAATACATTTATGTTTATAAGTTCTTTATTATTAATTAGTTGATTAATTAAAGTGAATATGTATATCAAAGTTTCATATAATAAATGGAGCTTGACATAGCCTCACAATCTATGTTATTGAATCAAAGTCTTGGAATTGATTGAAATGAAACATCTTGAAACAACACAAAGATTATTTCTAAGGTATTGAATTTAGGTGTTGAGGTTGATTGAATTAATCTAGTTTCAGTGTTTTTCCAACACCGGGTAATTTACATATTTGGGTTTTAGTTTATGGTTATAGGTGCCTTGCTAATTGGGCAACTAGATTACAAAATTGCGTTAAGATGTGACATATGCGACCTTAATTTCAGTTGAATTGCAATCATAGATACATAAATAATCTTGATGTTGTGTCTATGTCGCAGCCACGACCTAACTTTCAACTTTGATGTATACATGTGATTGAAAGTACTAGCTAGCTTATGATGAGAACAAATCTTTTCTGTAAGATTTTCACTAGAGGGAGTCCATAATGAAAATTTTCACCCTACTTAGGATTTTCACATTATAAAACTTTAAATGAGGTTAACTTGGCCTCACAATGTGTGTATGATTGAATCTAGGTGCCTTGCTAATTGGGTCACTAGTTTATGAAAATGGTGAAGATAGAGCTTTGTCCTGTTTTTGTCACAATTGTAGATTGCTTACACTTCAGCCGCCAACAACAATAATTGAAAGAAAGAAAACAAAAGTGAAATGCTTTTGAGGTATTGAAAGCTTTAGTTTAGGATCCGTACGTTATTATTGGTGTGTTTGTAAACAGAAAGGAAACTGACACAAAATGTAAGATAGAATTTTCCATGGTCTTTCTTAGTAATTAGGCAACAAGTCATCATGGATATTTCCTCATCATTCACACAAGACAGCTGTGTTAGTTTACATGACCAAAACAAGGTCTTTGCTATTTAGGCACAAAGAGACATTTTCTCTAACCATAAAACAAAAAATTGATTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTGCTATTTTGGTGCGTGTCTAAGAACTGTGTATTTGGTCTTGTGATCATGTAAGACTAGCTTCACTTATAAATTATATCAAATTTATATACATTTCATGCACAAAAATAAACTTGTCGAAATTGTATCTCACTAGTTAATTATTTTAATGGATTATATGCTATTATATTATATATGCACAAATTTATATATGCAAGATCCGGGGTTCAAACCTCGAACCCCGGCCACCACCAAAAAAAAAAAATTCACTTTTCTACACAATAGTTTGACACAATTATTAGGAAGTTAATATGGTATAATTCAGTTGTTAGATTGGTCAATTTTTTACATAAAATTATAAGTAGGTTAATTGATCATTTTCCTATATGTGTGTTTGTGTGTGACACATTGACCTAATTTGTTGTGCCTTCACATGCATTGGTAGCAGCAGGAAGAATGATTTGGAGAAGAGAACAGAGATGCATCCAGCACCTCAGATTGGAAACACTGAGATGCCACCAGAAGATGGCTTTACTTGGAGAAAATATGGCCAAAAAGAAATACTTGGCCGCAAGCACCCAAGGTAAATTAACAATAATTACTTAGAATTGTACTTTATAATATAGGGTAATAATTTCCATAAAGAGTTTGAAAGTTTCAATTCTAAGACAAATTCGTCTTTCATTATGCACCATCCAGAAAACCTTATACCTTTCTTCTAAGAATATTTCTTGGTCATGTTGAAAATTCAGTAGAACAACAAGTGTGTGCCCTATGAATTGACTCATTCACACATCATAACAAGACAAGTTGACTATTTACATTCATGTATAAGAGACAACTAGTCGCTTTTTCATCACAACTACTCAACTAGTATTAAAAAAAGAAAATACTAAGATGAATAAATTCATCACGTTTATTTTGCCTTGATTACTTGTTAAATATTTCTTTTTCTTTTTTTTAATAATTGATTAGCATTAAAAAAAAAATAATCTAAAACAGCAGTTTTGAATGAGAAATTGTCTTGAGTAGCTTATCTAAAAAAAATATTTTCAATAATTGATTTTCTTCAAAAAAAAAAATTGATTTCATTATAGTAAGCAACACTTAATTGTTTATTTTTTAAACATCTTGGTTTTTATTATATAATTTGTTGGCTAATTTGAAATATTTATTTCCTTTATCAGAAGTTACTATAGGTGCACACACCAAAAATTATATTTGTGTCCAGCCAAGAAGCAAGTGCAAAGACTTGATGAAAACCCTAACATAGTTGAAGTAACATATAGAGGTGAACACATTTGTCACAAATCCTTAACAGCACCATCATCATATCCACCACCACCAAACCTACTTGTGAACATTAGTACCATTGAAACTGCCATTTCTTCCCAAGGGTGGCCACCTGCGGTGAACCACGACCTCAGGGGCGGAGACGGTACTGGTGGACCCTCTACTTCAAGATTCGGCATTGATTATCCGGTGGCCGATATGGCCGATGTAATGTTCAACTCGGGTAGTAGCAGTGGCAATAGTATGGAATCACTCTTTTATCTAACTGAAGATAAAGGTGAACCTGATGAGGAGAAAAACTGA
Protein sequence

>MS.gene010021.t1

MEEVMNFIMRAFELAKNLELELPNMANHPEMLFSSIDDVEKAFNAAKERVMMMLSSQHDTTTTTTKPSNSFAQILSHDVFMQKTQHAQIGGSGSAISMHAHSMDQLLLMQHPFDVSVLIENKMISGGGDGGDVQLLRSRETLRIGEMGGRDMEGLDRSKGLEGDQMQAIETPPSRPKKCSRKNDLEKRTEMHPAPQIGNTEMPPEDGFTWRKYGQKEILGRKHPRSYYRCTHQKLYLCPAKKQVQRLDENPNIVEVTYRGEHICHKSLTAPSSYPPPPNLLVNISTIETAISSQGWPPAVNHDLRGGDGTGGPSTSRFGIDYPVADMADVMFNSGSSSGNSMESLFYLTEDKGEPDEEKN