AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010228

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010228.t1 MTR_5g061220 47.843 255 59 3 48 232 99 349 1.31e-65 212
MS.gene010228.t1 MTR_5g060630 48.927 233 72 4 47 232 120 352 1.91e-59 196
MS.gene010228.t1 MTR_5g460740 48.927 233 72 4 47 232 120 352 1.91e-59 196
MS.gene010228.t1 MTR_1g058210 49.362 235 67 4 45 232 91 320 1.04e-58 192
MS.gene010228.t1 MTR_5g061890 48.696 230 71 6 47 232 91 317 2.06e-56 185
MS.gene010228.t1 MTR_5g460920 47.391 230 74 6 47 232 91 317 1.72e-54 180
MS.gene010228.t1 MTR_5g060780 47.391 230 74 6 47 232 91 317 2.22e-54 180
MS.gene010228.t1 MTR_5g062960 84.444 90 14 0 143 232 1 90 1.97e-48 159
MS.gene010228.t1 MTR_5g062970 62.810 121 10 1 43 128 114 234 3.05e-43 147
MS.gene010228.t1 MTR_5g040740 41.228 228 84 8 48 231 94 315 2.55e-38 138
MS.gene010228.t1 MTR_5g040880 39.367 221 87 7 53 232 99 313 5.02e-37 136
MS.gene010228.t1 MTR_2g024430 40.870 230 88 8 45 232 100 323 3.48e-36 134
MS.gene010228.t1 MTR_5g060770 42.424 198 60 6 57 229 50 218 4.77e-33 123
MS.gene010228.t1 MTR_2g006270 40.351 228 79 9 49 229 98 315 5.56e-32 122
MS.gene010228.t1 MTR_5g082140 38.528 231 92 9 46 232 94 318 5.19e-31 119
MS.gene010228.t1 MTR_3g073420 33.333 267 87 6 52 232 101 362 1.18e-30 119
MS.gene010228.t1 MTR_2g094570 32.103 271 86 7 53 229 144 410 2.03e-29 116
MS.gene010228.t1 MTR_2g094570 32.103 271 86 7 53 229 88 354 2.05e-29 116
MS.gene010228.t1 MTR_2g094570 32.103 271 86 7 53 229 88 354 2.48e-29 116
MS.gene010228.t1 MTR_2g006380 46.154 156 67 8 84 232 175 320 7.22e-29 113
MS.gene010228.t1 MTR_7g101275 32.258 217 93 4 66 232 91 303 2.02e-28 111
MS.gene010228.t1 MTR_1g094960 32.000 250 81 5 65 229 116 361 1.20e-27 111
MS.gene010228.t1 MTR_0041s0120 29.565 230 94 6 66 232 91 315 1.17e-21 93.2
MS.gene010228.t1 MTR_1g064430 29.323 266 93 6 58 232 120 381 9.10e-20 88.6
MS.gene010228.t1 MTR_5g460910 35.000 180 77 6 57 229 67 213 8.35e-19 84.3
MS.gene010228.t1 MTR_2g014770 27.647 170 102 4 79 232 205 369 1.98e-17 81.6
MS.gene010228.t1 MTR_2g014770 27.647 170 102 4 79 232 205 369 2.00e-17 81.6
MS.gene010228.t1 MTR_4g058930 45.545 101 46 4 134 229 265 361 3.18e-15 75.1
MS.gene010228.t1 MTR_4g058930 38.835 103 28 1 65 132 107 209 8.64e-13 67.8
MS.gene010228.t1 MTR_4g058930 45.545 101 46 4 134 229 265 361 3.41e-15 75.1
MS.gene010228.t1 MTR_4g058930 38.835 103 28 1 65 132 107 209 9.29e-13 67.8
MS.gene010228.t1 MTR_8g100050 27.059 170 103 5 79 232 209 373 3.98e-14 72.0
MS.gene010228.t1 MTR_8g100050 27.059 170 103 5 79 232 209 373 4.06e-14 72.0
MS.gene010228.t1 MTR_4g088210 25.146 171 111 3 84 239 200 368 5.42e-14 71.6
MS.gene010228.t1 MTR_4g088210 25.146 171 111 3 84 239 200 368 5.55e-14 71.6
MS.gene010228.t1 MTR_4g021580 25.108 231 118 6 52 232 106 331 7.23e-14 71.2
MS.gene010228.t1 MTR_4g021580 25.108 231 118 6 52 232 106 331 1.01e-13 70.9
MS.gene010228.t1 MTR_4g060460 30.337 178 106 5 79 241 213 387 1.01e-13 70.9
MS.gene010228.t1 MTR_4g060460 30.337 178 106 5 79 241 213 387 1.04e-13 70.9
MS.gene010228.t1 MTR_2g018690 33.113 151 82 6 79 215 174 319 1.40e-13 70.5
MS.gene010228.t1 MTR_1g064430 43.000 100 50 3 136 232 299 394 1.52e-13 70.1
MS.gene010228.t1 MTR_5g076270 32.500 160 86 7 84 226 180 334 1.58e-13 70.1
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 85 217 2.26e-13 69.7
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 215 347 2.27e-13 69.7
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 98 230 2.32e-13 69.7
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 98 230 2.35e-13 69.7
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 228 360 2.58e-13 69.7
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 215 347 2.62e-13 69.7
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 228 360 2.70e-13 69.3
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 215 347 2.79e-13 69.3
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 85 217 2.82e-13 69.3
MS.gene010228.t1 MTR_2g093740 32.090 134 76 3 79 198 228 360 2.86e-13 69.3
MS.gene010228.t1 MTR_4g124900 29.012 162 98 4 82 229 176 334 9.36e-13 68.2
MS.gene010228.t1 MTR_8g079492 32.515 163 88 8 84 229 96 253 1.32e-12 67.4
MS.gene010228.t1 MTR_8g079492 32.515 163 88 8 84 229 178 335 1.35e-12 67.4
MS.gene010228.t1 MTR_8g079492 32.515 163 88 8 84 229 178 335 1.35e-12 67.4
MS.gene010228.t1 MTR_7g062540 28.049 164 98 6 84 232 168 326 1.93e-12 67.0
MS.gene010228.t1 MTR_2g005240 28.235 170 101 4 79 232 172 336 1.96e-12 67.0
MS.gene010228.t1 MTR_2g043250 28.070 171 102 6 82 236 176 341 3.69e-12 66.2
MS.gene010228.t1 MTR_3g064050 29.814 161 95 5 84 229 182 339 3.94e-12 66.2
MS.gene010228.t1 MTR_4g124900 29.375 160 96 4 84 229 178 334 6.55e-12 65.5
MS.gene010228.t1 MTR_1g024025 29.091 165 101 4 82 232 196 358 2.63e-11 63.5
MS.gene010228.t1 MTR_8g101360 33.000 100 54 1 84 170 187 286 6.39e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010228.t1 AT1G77850 30.830 253 92 4 59 232 111 359 1.95e-31 122
MS.gene010228.t1 AT1G77850 30.830 253 92 4 59 232 111 359 2.52e-31 121
MS.gene010228.t1 AT4G30080 30.827 266 91 6 56 232 104 365 3.13e-27 110
MS.gene010228.t1 AT2G28350 29.965 287 95 8 30 229 93 360 1.63e-24 102
MS.gene010228.t1 AT2G28350 29.965 287 95 8 30 229 93 360 1.78e-24 102
MS.gene010228.t1 AT5G37020 30.000 160 90 7 84 226 178 332 5.77e-15 74.3
MS.gene010228.t1 AT5G37020 30.000 160 90 7 84 226 83 237 6.23e-15 74.3
MS.gene010228.t1 AT2G33860 28.986 138 83 3 79 202 206 342 9.52e-15 73.6
MS.gene010228.t1 AT2G33860 28.986 138 83 3 79 202 49 185 1.11e-14 73.2
MS.gene010228.t1 AT5G37020 29.448 163 93 7 84 229 178 335 1.72e-14 73.2
MS.gene010228.t1 AT2G46530 27.273 220 103 7 63 232 33 245 1.88e-14 72.8
MS.gene010228.t1 AT2G46530 27.273 220 103 7 63 232 120 332 2.05e-14 72.8
MS.gene010228.t1 AT2G46530 27.273 220 103 7 63 232 141 353 2.07e-14 72.8
MS.gene010228.t1 AT3G61830 27.650 217 100 7 66 232 127 336 5.00e-14 71.6
MS.gene010228.t1 AT3G61830 27.650 217 100 7 66 232 76 285 5.20e-14 71.6
MS.gene010228.t1 AT1G59750 24.569 232 119 5 52 232 109 335 6.73e-14 71.2
MS.gene010228.t1 AT1G59750 24.569 232 119 5 52 232 109 335 6.73e-14 71.2
MS.gene010228.t1 AT1G59750 24.569 232 119 5 52 232 109 335 7.04e-14 71.2
MS.gene010228.t1 AT1G59750 24.569 232 119 5 52 232 109 335 7.24e-14 71.2
MS.gene010228.t1 AT1G30330 32.877 146 79 6 84 215 181 321 1.60e-13 70.1
MS.gene010228.t1 AT1G30330 32.877 146 79 6 84 215 181 321 1.60e-13 70.1
MS.gene010228.t1 AT1G30330 32.877 146 79 6 84 215 179 319 1.76e-13 70.1
MS.gene010228.t1 AT5G62000 25.316 237 117 8 47 232 148 375 1.88e-13 70.1
MS.gene010228.t1 AT5G62000 25.316 237 117 8 47 232 148 375 2.25e-13 69.7
MS.gene010228.t1 AT5G62000 25.316 237 117 8 47 232 148 375 2.25e-13 69.7
MS.gene010228.t1 AT5G62000 25.316 237 117 8 47 232 148 375 2.25e-13 69.7
MS.gene010228.t1 AT5G62000 25.316 237 117 8 47 232 148 375 2.25e-13 69.7
MS.gene010228.t1 AT5G20730 27.976 168 98 4 82 232 177 338 8.27e-12 65.1
MS.gene010228.t1 AT5G60450 27.219 169 103 6 79 232 200 363 1.58e-11 64.3
MS.gene010228.t1 AT5G60450 27.219 169 103 6 79 232 224 387 1.59e-11 64.3
MS.gene010228.t1 AT4G23980 27.068 133 82 3 79 198 163 293 2.25e-11 63.9
MS.gene010228.t1 AT4G23980 27.068 133 82 3 79 198 163 293 2.41e-11 63.5
MS.gene010228.t1 AT1G19220 27.607 163 99 4 82 229 176 334 2.52e-11 63.9

Find 60 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTAATTGGCGCATGCTTC+AGG 0.281500 5.1:-48414464 MS.gene010228:intron
CTGGAGGAGCTTCTTGTTCA+TGG 0.301419 5.1:+48415083 None:intergenic
CCTCACTCCCGTAACGGATA+TGG 0.302385 5.1:-48415112 MS.gene010228:CDS
CTCCTTCTAATGTCCCTAAT+TGG 0.322348 5.1:-48414477 MS.gene010228:CDS
ACCTGAAGCATGCGCCAATT+AGG 0.352249 5.1:+48414463 None:intergenic
GGTAACAGAGAAGGCGGTTA+TGG 0.361245 5.1:-48414722 MS.gene010228:CDS
GGTTATGGATGCGGTGGAAT+TGG 0.365128 5.1:-48414707 MS.gene010228:CDS
CCTGAAGCATGCGCCAATTA+GGG 0.377642 5.1:+48414464 None:intergenic
TCGTCTTCGGCAGGAACCTC+TGG 0.379847 5.1:+48415064 None:intergenic
ACACAATCTGATTGCAATAG+TGG 0.402033 5.1:-48415004 MS.gene010228:CDS
ATGAAGATTAGTTGGACATC+TGG 0.423644 5.1:-48414595 MS.gene010228:CDS
CGCCAATTAGGGACATTAGA+AGG 0.425529 5.1:+48414475 None:intergenic
AGATGTCCAACTAATCTTCA+TGG 0.435291 5.1:+48414597 None:intergenic
TACGGGAGTGAGGAGAAGTT+TGG 0.442803 5.1:+48415122 None:intergenic
GGCCATGGCCATATCCGTTA+CGG 0.443013 5.1:+48415104 None:intergenic
TTACAGTTCAAGTTTATAGT+TGG 0.445991 5.1:-48414360 MS.gene010228:intron
GCCATGGCCATATCCGTTAC+GGG 0.450102 5.1:+48415105 None:intergenic
TGTATTATCCGAGAGCGAAT+TGG 0.460538 5.1:-48414657 MS.gene010228:CDS
ATGATGCCATGAAGATTAGT+TGG 0.464606 5.1:-48414603 MS.gene010228:CDS
TCAGCTGGGAAGATATTCAT+TGG 0.466423 5.1:-48414766 MS.gene010228:CDS
GAAGATTCTTCTTTCTTCAA+AGG 0.471685 5.1:+48414556 None:intergenic
AAGTCCGTCCCCTACTGAAA+AGG 0.479589 5.1:+48414845 None:intergenic
ATTGTAGCCATCCTCGTCTT+CGG 0.480424 5.1:+48415051 None:intergenic
CCTACTGAAAAGGTGTCTCT+TGG 0.492652 5.1:+48414855 None:intergenic
TCAAAGAAATCATCGAATTG+TGG 0.506724 5.1:+48414324 None:intergenic
TAGCCATCCTCGTCTTCGGC+AGG 0.513990 5.1:+48415055 None:intergenic
AATGTAGCTCCCCGGTACTA+CGG 0.515806 5.1:+48415312 None:intergenic
GATGAGATTGTATCAGCCTC+GGG 0.518035 5.1:-48414524 MS.gene010228:CDS
TGTGCATAAATCCGTAGTAC+CGG 0.519479 5.1:-48415323 MS.gene010228:CDS
ATTGGAATATGCCGGAAAAG+TGG 0.534162 5.1:-48414748 MS.gene010228:CDS
AGAGAAGGCGGTTATGGATG+CGG 0.535241 5.1:-48414716 MS.gene010228:CDS
GAGCTTCTTGTTCATGGCCA+TGG 0.538260 5.1:+48415089 None:intergenic
GTGCATAAATCCGTAGTACC+GGG 0.539498 5.1:-48415322 MS.gene010228:CDS
AGATGAGATTGTATCAGCCT+CGG 0.541379 5.1:-48414525 MS.gene010228:CDS
AGATATTCATTGGAATATGC+CGG 0.543727 5.1:-48414756 MS.gene010228:CDS
AAATCAAACCAATTCGCTCT+CGG 0.544399 5.1:+48414649 None:intergenic
TATGGATGCGGTGGAATTGG+CGG 0.548189 5.1:-48414704 MS.gene010228:CDS
TTGGAATATGCCGGAAAAGT+GGG 0.549503 5.1:-48414747 MS.gene010228:CDS
AACAACAAGAAACTCGTCGC+TGG 0.566110 5.1:-48414814 MS.gene010228:CDS
TCTGATTGCAATAGTGGCTG+TGG 0.575315 5.1:-48414998 MS.gene010228:CDS
CATGTTCTAAGTGACCTTGT+GGG 0.576431 5.1:+48415253 None:intergenic
TCCCGTAACGGATATGGCCA+TGG 0.578270 5.1:-48415106 MS.gene010228:CDS
TGCTTCGGAATGTAGCTCCC+CGG 0.594410 5.1:+48415304 None:intergenic
ACTTCTCCTCACTCCCGTAA+CGG 0.594736 5.1:-48415118 MS.gene010228:CDS
TCTTCGGCAGGAACCTCTGG+AGG 0.611963 5.1:+48415067 None:intergenic
AGAGGTTCCTGCCGAAGACG+AGG 0.616143 5.1:-48415062 MS.gene010228:CDS
CCATATCCGTTACGGGAGTG+AGG 0.628397 5.1:+48415112 None:intergenic
AAGTGGGAAGGTAACAGAGA+AGG 0.628618 5.1:-48414731 MS.gene010228:CDS
GTTCCTGCCGAAGACGAGGA+TGG 0.629556 5.1:-48415058 MS.gene010228:CDS
GAAGGCGGTTATGGATGCGG+TGG 0.634390 5.1:-48414713 MS.gene010228:CDS
TTGTGTGAGAATCTTGACAA+AGG 0.644198 5.1:+48415021 None:intergenic
TGCATAAATCCGTAGTACCG+GGG 0.668775 5.1:-48415321 MS.gene010228:CDS
TGGGAAGGTAACAGAGAAGG+CGG 0.684452 5.1:-48414728 MS.gene010228:CDS
TATATTTACTACTTCCCACA+AGG 0.684952 5.1:-48415267 MS.gene010228:intron
ATGAGATTGTATCAGCCTCG+GGG 0.686243 5.1:-48414523 MS.gene010228:CDS
TGAACAAGAAGCTCCTCCAG+AGG 0.697806 5.1:-48415080 MS.gene010228:CDS
TGAGATTGTATCAGCCTCGG+GGG 0.706802 5.1:-48414522 MS.gene010228:CDS
GGTGTCTCTTGGATTTACCG+CGG 0.707333 5.1:+48414866 None:intergenic
AATATGCCGGAAAAGTGGGA+AGG 0.715565 5.1:-48414743 MS.gene010228:CDS
CAGTACGCGCATATCTACCG+CGG 0.748699 5.1:-48414883 MS.gene010228:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTAGTAAATATAGATTTTG+TGG + chr5.1:48414368-48414387 None:intergenic 20.0%
!!! GTAGTAAATATAGATTTTGT+GGG + chr5.1:48414367-48414386 None:intergenic 20.0%
!!! TAGTAAATATAGATTTTGTG+GGG + chr5.1:48414366-48414385 None:intergenic 20.0%
!! TTACAGTTCAAGTTTATAGT+TGG - chr5.1:48415282-48415301 MS.gene010228:CDS 25.0%
AGATATTCATTGGAATATGC+CGG - chr5.1:48414886-48414905 MS.gene010228:intron 30.0%
GAAGATTCTTCTTTCTTCAA+AGG + chr5.1:48415089-48415108 None:intergenic 30.0%
TATATTTACTACTTCCCACA+AGG - chr5.1:48414375-48414394 MS.gene010228:intron 30.0%
TCAAAGAAATCATCGAATTG+TGG + chr5.1:48415321-48415340 None:intergenic 30.0%
! TTTTCGTTAAAAACTCAGCT+GGG - chr5.1:48414862-48414881 MS.gene010228:CDS 30.0%
AAATCAAACCAATTCGCTCT+CGG + chr5.1:48414996-48415015 None:intergenic 35.0%
ACACAATCTGATTGCAATAG+TGG - chr5.1:48414638-48414657 MS.gene010228:CDS 35.0%
AGATGTCCAACTAATCTTCA+TGG + chr5.1:48415048-48415067 None:intergenic 35.0%
ATGATGCCATGAAGATTAGT+TGG - chr5.1:48415039-48415058 MS.gene010228:CDS 35.0%
TTGTGTGAGAATCTTGACAA+AGG + chr5.1:48414624-48414643 None:intergenic 35.0%
! ATGAAGATTAGTTGGACATC+TGG - chr5.1:48415047-48415066 MS.gene010228:CDS 35.0%
! GTACATACGCTTTCTTCTTT+TGG - chr5.1:48415200-48415219 MS.gene010228:intron 35.0%
! GTTTTCGTTAAAAACTCAGC+TGG - chr5.1:48414861-48414880 MS.gene010228:CDS 35.0%
AAGGAGGATAACGATGAAGA+AGG + chr5.1:48414437-48414456 None:intergenic 40.0%
AGATGAGATTGTATCAGCCT+CGG - chr5.1:48415117-48415136 MS.gene010228:CDS 40.0%
AGGAGGATAACGATGAAGAA+GGG + chr5.1:48414436-48414455 None:intergenic 40.0%
ATTAACGACTGAGATGATGC+AGG + chr5.1:48414466-48414485 None:intergenic 40.0%
ATTGGAATATGCCGGAAAAG+TGG - chr5.1:48414894-48414913 MS.gene010228:intron 40.0%
CTCCTTCTAATGTCCCTAAT+TGG - chr5.1:48415165-48415184 MS.gene010228:intron 40.0%
GAATCGTCCAAGTTAAGCTT+CGG + chr5.1:48414690-48414709 None:intergenic 40.0%
GCAAATACTACATCTGTGTG+AGG + chr5.1:48414501-48414520 None:intergenic 40.0%
TCAGCTGGGAAGATATTCAT+TGG - chr5.1:48414876-48414895 MS.gene010228:CDS 40.0%
TGTATTATCCGAGAGCGAAT+TGG - chr5.1:48414985-48415004 MS.gene010228:intron 40.0%
TGTGCATAAATCCGTAGTAC+CGG - chr5.1:48414319-48414338 MS.gene010228:CDS 40.0%
TTGGAATATGCCGGAAAAGT+GGG - chr5.1:48414895-48414914 MS.gene010228:intron 40.0%
! AAGAGACACCTTTTCAGTAG+GGG - chr5.1:48414789-48414808 MS.gene010228:CDS 40.0%
! ACATGTTCTAAGTGACCTTG+TGG + chr5.1:48414393-48414412 None:intergenic 40.0%
! AGATTTTGTGGGGAATGCTT+CGG + chr5.1:48414356-48414375 None:intergenic 40.0%
! CAAGAGACACCTTTTCAGTA+GGG - chr5.1:48414788-48414807 MS.gene010228:CDS 40.0%
! CATGTTCTAAGTGACCTTGT+GGG + chr5.1:48414392-48414411 None:intergenic 40.0%
AACAACAAGAAACTCGTCGC+TGG - chr5.1:48414828-48414847 MS.gene010228:CDS 45.0%
AAGGGTGATGATTGTGTGAG+CGG + chr5.1:48414418-48414437 None:intergenic 45.0%
AAGTGGGAAGGTAACAGAGA+AGG - chr5.1:48414911-48414930 MS.gene010228:intron 45.0%
AATATGCCGGAAAAGTGGGA+AGG - chr5.1:48414899-48414918 MS.gene010228:intron 45.0%
ATGAGATTGTATCAGCCTCG+GGG - chr5.1:48415119-48415138 MS.gene010228:CDS 45.0%
ATGATGCAGGAAATGGAAGG+AGG + chr5.1:48414453-48414472 None:intergenic 45.0%
ATTGTAGCCATCCTCGTCTT+CGG + chr5.1:48414594-48414613 None:intergenic 45.0%
CATCTGTGTGAGGATCAACA+AGG + chr5.1:48414491-48414510 None:intergenic 45.0%
CGCCAATTAGGGACATTAGA+AGG + chr5.1:48415170-48415189 None:intergenic 45.0%
GACTGAGATGATGCAGGAAA+TGG + chr5.1:48414460-48414479 None:intergenic 45.0%
GAGATGATGCAGGAAATGGA+AGG + chr5.1:48414456-48414475 None:intergenic 45.0%
GATGAGATTGTATCAGCCTC+GGG - chr5.1:48415118-48415137 MS.gene010228:CDS 45.0%
GGAATTGGCGGTGAAAAACA+TGG - chr5.1:48414950-48414969 MS.gene010228:intron 45.0%
GTGCATAAATCCGTAGTACC+GGG - chr5.1:48414320-48414339 MS.gene010228:CDS 45.0%
TCAGCCTTGATTGTCAGTGA+CGG + chr5.1:48414733-48414752 None:intergenic 45.0%
TCCAAGTTAAGCTTCGGCAA+GGG + chr5.1:48414684-48414703 None:intergenic 45.0%
TCTGATTGCAATAGTGGCTG+TGG - chr5.1:48414644-48414663 MS.gene010228:CDS 45.0%
TGCATAAATCCGTAGTACCG+GGG - chr5.1:48414321-48414340 MS.gene010228:CDS 45.0%
! CCAAGAGACACCTTTTCAGT+AGG - chr5.1:48414787-48414806 MS.gene010228:CDS 45.0%
!! CCTACTGAAAAGGTGTCTCT+TGG + chr5.1:48414790-48414809 None:intergenic 45.0%
!! CTGTTACCTTCCCACTTTTC+CGG + chr5.1:48414908-48414927 None:intergenic 45.0%
AAGTCCGTCCCCTACTGAAA+AGG + chr5.1:48414800-48414819 None:intergenic 50.0%
AATGTAGCTCCCCGGTACTA+CGG + chr5.1:48414333-48414352 None:intergenic 50.0%
ACCTGAAGCATGCGCCAATT+AGG + chr5.1:48415182-48415201 None:intergenic 50.0%
ACTTCTCCTCACTCCCGTAA+CGG - chr5.1:48414524-48414543 MS.gene010228:CDS 50.0%
AGAGAAGGCGGTTATGGATG+CGG - chr5.1:48414926-48414945 MS.gene010228:intron 50.0%
CCTGAAGCATGCGCCAATTA+GGG + chr5.1:48415181-48415200 None:intergenic 50.0%
CTCTCCGTCACTGACAATCA+AGG - chr5.1:48414726-48414745 MS.gene010228:CDS 50.0%
CTGACAATCAAGGCTGAGTC+TGG - chr5.1:48414736-48414755 MS.gene010228:CDS 50.0%
CTGGAGGAGCTTCTTGTTCA+TGG + chr5.1:48414562-48414581 None:intergenic 50.0%
GAGCTTCTTGTTCATGGCCA+TGG + chr5.1:48414556-48414575 None:intergenic 50.0%
GGTAACAGAGAAGGCGGTTA+TGG - chr5.1:48414920-48414939 MS.gene010228:intron 50.0%
GGTGTCTCTTGGATTTACCG+CGG + chr5.1:48414779-48414798 None:intergenic 50.0%
GGTTATGGATGCGGTGGAAT+TGG - chr5.1:48414935-48414954 MS.gene010228:intron 50.0%
GTCCAAGTTAAGCTTCGGCA+AGG + chr5.1:48414685-48414704 None:intergenic 50.0%
TACGGGAGTGAGGAGAAGTT+TGG + chr5.1:48414523-48414542 None:intergenic 50.0%
TATGGATGCGGTGGAATTGG+CGG - chr5.1:48414938-48414957 MS.gene010228:intron 50.0%
TCCCTTGCCGAAGCTTAACT+TGG - chr5.1:48414680-48414699 MS.gene010228:CDS 50.0%
TGAACAAGAAGCTCCTCCAG+AGG - chr5.1:48414562-48414581 MS.gene010228:CDS 50.0%
TGAGATTGTATCAGCCTCGG+GGG - chr5.1:48415120-48415139 MS.gene010228:CDS 50.0%
TGGGAAGGTAACAGAGAAGG+CGG - chr5.1:48414914-48414933 MS.gene010228:intron 50.0%
! GACACCTTTTCAGTAGGGGA+CGG - chr5.1:48414793-48414812 MS.gene010228:CDS 50.0%
CAGTACGCGCATATCTACCG+CGG - chr5.1:48414759-48414778 MS.gene010228:CDS 55.0%
CCATATCCGTTACGGGAGTG+AGG + chr5.1:48414533-48414552 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTC+AGG - chr5.1:48415178-48415197 MS.gene010228:intron 55.0%
GCCATGGCCATATCCGTTAC+GGG + chr5.1:48414540-48414559 None:intergenic 55.0%
GGCCATGGCCATATCCGTTA+CGG + chr5.1:48414541-48414560 None:intergenic 55.0%
TGCTTCGGAATGTAGCTCCC+CGG + chr5.1:48414341-48414360 None:intergenic 55.0%
! ACATCTGAGATTGTCCCCCG+AGG + chr5.1:48415137-48415156 None:intergenic 55.0%
!! CCTCACTCCCGTAACGGATA+TGG - chr5.1:48414530-48414549 MS.gene010228:CDS 55.0%
!! GGAGAGTGTCTGAGATCGCA+TGG + chr5.1:48414712-48414731 None:intergenic 55.0%
!! TCCCGTAACGGATATGGCCA+TGG - chr5.1:48414536-48414555 MS.gene010228:CDS 55.0%
AGAGGTTCCTGCCGAAGACG+AGG - chr5.1:48414580-48414599 MS.gene010228:CDS 60.0%
GAAGGCGGTTATGGATGCGG+TGG - chr5.1:48414929-48414948 MS.gene010228:intron 60.0%
GTTCCTGCCGAAGACGAGGA+TGG - chr5.1:48414584-48414603 MS.gene010228:CDS 60.0%
TAGCCATCCTCGTCTTCGGC+AGG + chr5.1:48414590-48414609 None:intergenic 60.0%
TCGTCTTCGGCAGGAACCTC+TGG + chr5.1:48414581-48414600 None:intergenic 60.0%
TCTTCGGCAGGAACCTCTGG+AGG + chr5.1:48414578-48414597 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 48414314 48415350 48414314 ID=MS.gene010228
chr5.1 mRNA 48414314 48415350 48414314 ID=MS.gene010228.t1;Parent=MS.gene010228
chr5.1 exon 48415268 48415350 48415268 ID=MS.gene010228.t1.exon1;Parent=MS.gene010228.t1
chr5.1 CDS 48415268 48415350 48415268 ID=cds.MS.gene010228.t1;Parent=MS.gene010228.t1
chr5.1 exon 48414991 48415156 48414991 ID=MS.gene010228.t1.exon2;Parent=MS.gene010228.t1
chr5.1 CDS 48414991 48415156 48414991 ID=cds.MS.gene010228.t1;Parent=MS.gene010228.t1
chr5.1 exon 48414465 48414902 48414465 ID=MS.gene010228.t1.exon3;Parent=MS.gene010228.t1
chr5.1 CDS 48414465 48414902 48414465 ID=cds.MS.gene010228.t1;Parent=MS.gene010228.t1
chr5.1 exon 48414314 48414376 48414314 ID=MS.gene010228.t1.exon4;Parent=MS.gene010228.t1
chr5.1 CDS 48414314 48414376 48414314 ID=cds.MS.gene010228.t1;Parent=MS.gene010228.t1
Gene Sequence

>MS.gene010228

ATGCATGTGCATAAATCCGTAGTACCGGGGAGCTACATTCCGAAGCATTCCCCACAAAATCTATATTTACTACTTCCCACAAGGTCACTTAGAACATGTCTCCGCTCACACAATCATCACCCTTCTTCATCGTTATCCTCCTTCCATTTCCTGCATCATCTCAGTCGTTAATCTCCTTGTTGATCCTCACACAGATGTAGTATTTGCCAAACTTCTCCTCACTCCCGTAACGGATATGGCCATGGCCATGAACAAGAAGCTCCTCCAGAGGTTCCTGCCGAAGACGAGGATGGCTACAATGTTGTTTCCTTTGTCAAGATTCTCACACAATCTGATTGCAATAGTGGCTGTGGATTCATTGTACTCTCCCTTGCCGAAGCTTAACTTGGACGATTCCATGCGATCTCAGACACTCTCCGTCACTGACAATCAAGGCTGAGTCTGGCAGTACGCGCATATCTACCGCGGTAAATCCAAGAGACACCTTTTCAGTAGGGGACGGACTTCATTTGTCAACAACAAGAAACTCGTCGCTGGTGATTCAGTTGTTTTCGTTAAAAACTCAGCTGGGAAGATATTCATTGGAATATGCCGGAAAAGTGGGAAGGTAACAGAGAAGGCGGTTATGGATGCGGTGGAATTGGCGGTGAAAAACATGGCATTTGAAGTTTTGTATTATCCGAGAGCGAATTGGTTTGATTTTGTAGTGCATGCAAATGTTGTTGATGATGCCATGAAGATTAGTTGGACATCTGGAATTCAAGTCAAGCTTCCTTTGAAGAAAGAAGAATCTTCAAATTCAAAGATGAGATTGTATCAGCCTCGGGGGACAATCTCAGATGTTTATAATGCTCCTTCTAATGTCCCTAATTGGCGCATGCTTCAGGTACATACGCTTTCTTCTTTTGGTCTTATTATATTTCAAGTGCATAATGTTATCTAGTACTACTTTTGTTTGATAATAAATATTACAGTTCAAGTTTATAGTTGGTACAAAGTTGAATCCACAATTCGATGATTTCTTTGATAAATATTAG
Protein sequence

>MS.gene010228.t1

MHVHKSVVPGSYIPKHSPQNLYLLLPTRCSICQTSPHSRNGYGHGHEQEAPPEVPAEDEDGYNVVSFVKILTQSDCNSGCGFIYAHIYRGKSKRHLFSRGRTSFVNNKKLVAGDSVVFVKNSAGKIFIGICRKSGKVTEKAVMDAVELAVKNMAFEVLYYPRANWFDFVVHANVVDDAMKISWTSGIQVKLPLKKEESSNSKMRLYQPRGTISDVYNAPSNVPNWRMLQFKFIVGTKLNPQFDDFFDKY