AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010286

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010286.t1 MTR_5g061220 92.250 529 39 2 1 528 1 528 0.0 1005
MS.gene010286.t1 MTR_1g058210 53.920 523 163 8 8 526 1 449 3.49e-177 507
MS.gene010286.t1 MTR_2g024430 49.817 546 211 14 6 526 7 514 4.97e-158 460
MS.gene010286.t1 MTR_5g040880 49.360 547 209 11 14 528 6 516 2.96e-156 456
MS.gene010286.t1 MTR_5g082140 48.276 522 188 11 14 522 7 459 5.21e-150 437
MS.gene010286.t1 MTR_3g073420 45.811 561 243 16 13 527 13 558 2.61e-144 428
MS.gene010286.t1 MTR_5g060630 47.339 545 188 13 8 480 1 518 1.88e-141 418
MS.gene010286.t1 MTR_5g460740 47.339 545 188 13 8 480 1 518 1.88e-141 418
MS.gene010286.t1 MTR_2g006270 47.002 517 189 13 15 523 9 448 6.81e-133 394
MS.gene010286.t1 MTR_5g060780 53.283 396 137 11 8 392 1 359 1.65e-128 380
MS.gene010286.t1 MTR_5g061890 53.944 393 139 10 8 392 1 359 3.37e-128 380
MS.gene010286.t1 MTR_5g460920 53.283 396 137 11 8 392 1 359 3.14e-127 377
MS.gene010286.t1 MTR_2g006380 42.994 521 197 16 15 523 16 448 3.99e-115 348
MS.gene010286.t1 MTR_5g040740 42.395 526 163 12 15 527 8 406 1.49e-113 343
MS.gene010286.t1 MTR_1g064430 41.286 482 222 12 14 452 35 498 1.27e-101 321
MS.gene010286.t1 MTR_1g064430 41.286 482 222 12 14 452 22 485 1.28e-101 321
MS.gene010286.t1 MTR_2g094570 40.491 489 227 15 19 464 11 478 5.57e-100 316
MS.gene010286.t1 MTR_2g094570 40.491 489 227 15 19 464 11 478 1.10e-99 316
MS.gene010286.t1 MTR_2g094570 40.491 489 227 15 19 464 67 534 4.65e-99 316
MS.gene010286.t1 MTR_4g058930 41.447 456 212 13 19 435 12 451 8.03e-97 308
MS.gene010286.t1 MTR_1g094960 39.915 471 216 14 7 432 7 455 1.16e-96 306
MS.gene010286.t1 MTR_4g058930 41.447 456 212 13 19 435 12 451 2.18e-96 307
MS.gene010286.t1 MTR_7g101280 38.655 476 218 13 14 438 26 478 2.00e-95 303
MS.gene010286.t1 MTR_0041s0110 38.655 476 218 13 14 438 26 478 2.00e-95 303
MS.gene010286.t1 MTR_5g062970 61.111 198 69 3 16 208 35 229 4.77e-79 248
MS.gene010286.t1 MTR_5g062960 62.814 199 46 4 264 456 1 177 2.57e-73 231
MS.gene010286.t1 MTR_5g060770 41.237 388 131 12 68 438 7 314 2.33e-70 229
MS.gene010286.t1 MTR_7g101275 40.303 330 154 9 29 346 2 300 1.85e-69 228
MS.gene010286.t1 MTR_0041s0120 39.701 335 171 10 29 351 2 317 2.53e-67 223
MS.gene010286.t1 MTR_2g014770 33.773 379 195 9 10 367 44 387 2.96e-64 220
MS.gene010286.t1 MTR_2g014770 33.773 379 195 9 10 367 44 387 5.78e-64 221
MS.gene010286.t1 MTR_2g005240 35.092 379 208 7 19 386 23 374 3.00e-61 213
MS.gene010286.t1 MTR_8g100050 34.456 386 202 10 19 386 59 411 5.33e-61 214
MS.gene010286.t1 MTR_8g100050 34.456 386 202 10 19 386 59 411 1.65e-60 214
MS.gene010286.t1 MTR_4g088210 34.140 372 189 9 19 367 38 376 3.93e-60 211
MS.gene010286.t1 MTR_4g088210 34.140 372 189 9 19 367 38 376 5.36e-60 211
MS.gene010286.t1 MTR_2g018690 35.149 404 205 12 6 385 6 376 1.01e-59 212
MS.gene010286.t1 MTR_4g124900 34.316 373 195 6 14 369 18 357 1.23e-59 213
MS.gene010286.t1 MTR_4g124900 34.316 373 195 6 14 369 18 357 1.28e-59 213
MS.gene010286.t1 MTR_4g021580 34.555 382 207 10 19 386 19 371 6.98e-59 206
MS.gene010286.t1 MTR_4g021580 34.555 382 207 10 19 386 19 371 8.51e-59 206
MS.gene010286.t1 MTR_2g043250 33.244 373 199 6 14 369 18 357 8.52e-59 211
MS.gene010286.t1 MTR_2g093740 34.184 392 205 12 19 389 56 415 1.12e-58 207
MS.gene010286.t1 MTR_2g093740 34.184 392 205 12 19 389 56 415 2.98e-58 206
MS.gene010286.t1 MTR_2g093740 34.184 392 205 12 19 389 56 415 4.66e-58 207
MS.gene010286.t1 MTR_5g076270 33.590 390 206 10 16 385 21 377 5.90e-58 207
MS.gene010286.t1 MTR_3g064050 32.821 390 209 9 16 385 23 379 6.99e-58 207
MS.gene010286.t1 MTR_8g079492 34.250 400 213 11 6 385 6 375 4.60e-57 205
MS.gene010286.t1 MTR_8g079492 34.250 400 213 11 6 385 6 375 4.91e-57 205
MS.gene010286.t1 MTR_1g024025 34.146 369 198 8 14 367 38 376 1.58e-56 204
MS.gene010286.t1 MTR_7g062540 32.225 391 223 10 19 396 14 375 5.15e-56 199
MS.gene010286.t1 MTR_8g101360 34.146 369 201 9 16 370 28 368 1.98e-55 201
MS.gene010286.t1 MTR_2g093740 32.593 405 207 12 19 389 56 428 3.92e-55 197
MS.gene010286.t1 MTR_2g093740 33.086 405 205 13 19 389 56 428 8.79e-55 197
MS.gene010286.t1 MTR_2g093740 33.086 405 205 13 19 389 56 428 1.02e-54 197
MS.gene010286.t1 MTR_8g027440 31.481 432 229 12 19 439 29 404 1.81e-54 195
MS.gene010286.t1 MTR_4g060460 34.694 392 203 11 19 386 54 416 4.64e-53 192
MS.gene010286.t1 MTR_4g060460 34.694 392 203 11 19 386 54 416 6.08e-53 192
MS.gene010286.t1 MTR_5g460910 34.085 399 143 15 58 438 13 309 1.55e-44 160
MS.gene010286.t1 MTR_8g079492 33.856 319 171 8 79 385 3 293 3.26e-43 164
MS.gene010286.t1 MTR_2g093740 33.444 302 155 8 102 389 16 285 6.48e-42 159
MS.gene010286.t1 MTR_2g093740 33.444 302 155 8 102 389 16 285 1.16e-41 159
MS.gene010286.t1 MTR_2g093740 32.063 315 155 9 102 389 16 298 1.72e-38 149
MS.gene010286.t1 MTR_2g093740 32.063 315 155 9 102 389 16 298 2.63e-38 149
MS.gene010286.t1 MTR_8g446900 28.431 306 180 9 70 367 14 288 6.11e-32 127
MS.gene010286.t1 MTR_5g046120 39.130 115 57 4 57 163 12 121 1.33e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010286.t1 AT1G77850 39.199 574 285 13 1 527 1 557 4.39e-123 373
MS.gene010286.t1 AT1G77850 39.199 574 285 13 1 527 1 557 6.00e-123 373
MS.gene010286.t1 AT4G30080 39.885 524 261 17 19 504 19 526 1.07e-95 305
MS.gene010286.t1 AT2G28350 39.655 464 207 13 19 432 10 450 6.33e-89 287
MS.gene010286.t1 AT2G28350 40.000 465 204 14 19 432 10 450 9.82e-89 287
MS.gene010286.t1 AT2G46530 36.565 361 194 8 18 370 20 353 2.58e-65 223
MS.gene010286.t1 AT2G46530 36.592 358 192 8 18 367 41 371 5.56e-65 223
MS.gene010286.t1 AT3G61830 34.574 376 201 9 15 382 21 359 4.29e-62 214
MS.gene010286.t1 AT2G33860 34.865 370 185 8 19 367 54 388 5.90e-62 214
MS.gene010286.t1 AT5G37020 33.168 404 218 10 16 400 19 389 9.61e-61 214
MS.gene010286.t1 AT5G37020 33.168 404 218 10 16 400 19 389 1.90e-60 213
MS.gene010286.t1 AT1G30330 35.369 393 199 11 14 385 18 376 4.56e-60 214
MS.gene010286.t1 AT1G30330 35.369 393 199 11 14 385 20 378 4.95e-60 214
MS.gene010286.t1 AT1G30330 35.369 393 199 11 14 385 20 378 4.95e-60 214
MS.gene010286.t1 AT5G62000 32.832 399 223 9 3 386 45 413 3.55e-58 207
MS.gene010286.t1 AT5G62000 32.832 399 223 9 3 386 45 413 3.55e-58 207
MS.gene010286.t1 AT5G62000 32.832 399 223 9 3 386 45 413 3.55e-58 207
MS.gene010286.t1 AT5G62000 32.832 399 223 9 3 386 45 413 3.55e-58 207
MS.gene010286.t1 AT5G62000 32.832 399 223 9 3 386 45 413 3.58e-58 207
MS.gene010286.t1 AT5G60450 34.860 393 199 14 13 383 35 392 3.96e-58 206
MS.gene010286.t1 AT4G23980 30.247 486 283 14 19 484 12 461 4.10e-58 204
MS.gene010286.t1 AT5G60450 34.872 390 197 14 16 383 62 416 8.79e-58 206
MS.gene010286.t1 AT5G20730 33.867 375 194 7 14 369 19 358 1.75e-57 207
MS.gene010286.t1 AT1G59750 32.877 365 206 6 19 372 22 358 2.25e-57 202
MS.gene010286.t1 AT1G59750 32.877 365 206 6 19 372 22 358 2.25e-57 202
MS.gene010286.t1 AT1G59750 32.877 365 206 6 19 372 22 358 2.35e-57 202
MS.gene010286.t1 AT1G59750 32.877 365 206 6 19 372 22 358 2.38e-57 202
MS.gene010286.t1 AT4G23980 33.506 385 213 9 19 390 12 366 4.72e-57 201
MS.gene010286.t1 AT1G19220 32.992 391 207 8 16 386 20 375 1.26e-56 204
MS.gene010286.t1 AT5G20730 33.777 376 194 8 14 369 19 359 8.03e-56 202
MS.gene010286.t1 AT5G20730 33.777 376 194 8 14 369 19 359 8.25e-56 202
MS.gene010286.t1 AT1G19850 34.511 368 195 9 16 367 40 377 4.87e-55 199
MS.gene010286.t1 AT1G19850 34.511 368 195 9 16 367 51 388 6.59e-55 199
MS.gene010286.t1 AT1G34310 32.383 386 217 9 16 390 24 376 8.39e-54 191
MS.gene010286.t1 AT1G34390 31.701 388 217 10 19 398 27 374 1.34e-53 191
MS.gene010286.t1 AT1G35520 32.376 383 215 10 19 390 27 376 1.11e-52 188
MS.gene010286.t1 AT1G34410 30.730 397 232 10 19 406 27 389 1.64e-52 188
MS.gene010286.t1 AT1G35540 31.759 381 220 9 19 390 27 376 9.54e-52 186
MS.gene010286.t1 AT1G35240 30.591 389 229 9 19 399 27 382 1.11e-49 180
MS.gene010286.t1 AT3G61830 33.934 333 179 8 54 382 13 308 6.31e-48 175
MS.gene010286.t1 AT1G34170 31.335 367 195 11 19 367 25 352 2.59e-46 169
MS.gene010286.t1 AT2G46530 36.434 258 138 4 114 370 34 266 4.43e-45 166
MS.gene010286.t1 AT2G33860 34.615 260 131 5 117 367 2 231 5.85e-44 162
MS.gene010286.t1 AT1G34170 26.679 521 271 18 19 508 25 465 2.28e-41 156
MS.gene010286.t1 AT5G37020 33.106 293 158 7 117 400 31 294 6.88e-41 157
MS.gene010286.t1 AT1G34170 29.700 367 175 11 19 367 25 326 5.98e-40 151
MS.gene010286.t1 AT1G43950 38.049 205 111 6 19 216 27 222 1.77e-36 135

Find 148 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGTTCAATTAAGTTATT+TGG 0.154755 5.4:-47756333 MS.gene010286:CDS
GGCACATGTCTGCCAGATTT+TGG 0.193688 5.4:+47759169 None:intergenic
TTCAAACTACTAAGGTTATT+TGG 0.193694 5.4:+47756411 None:intergenic
TCTCTTTATCATAGGTTAAC+TGG 0.215844 5.4:-47756788 MS.gene010286:intron
GTTTGGTTCAAAGATCTCTT+AGG 0.241864 5.4:+47756588 None:intergenic
CTTAGTAGTTTGAACATATT+CGG 0.242799 5.4:-47756402 MS.gene010286:CDS
CCCTAATTGGCGCATGCTTC+AGG 0.273598 5.4:-47758205 MS.gene010286:intron
CATGATCATTTATCTCTATC+AGG 0.276134 5.4:-47756519 MS.gene010286:CDS
ACAGTGATTGTTTGAGGGTT+AGG 0.284120 5.4:+47759065 None:intergenic
GAAAGTGATGTCCATGATTC+TGG 0.299846 5.4:-47756291 MS.gene010286:CDS
TATATTACCCTAGGGCTAAT+TGG 0.305719 5.4:-47758386 MS.gene010286:CDS
AATCAAAGGCAGTGAATAAT+AGG 0.317388 5.4:-47758548 MS.gene010286:CDS
GTCAATGCATCAAATTTCAT+TGG 0.331745 5.4:+47756239 None:intergenic
CTGATGTTCATGACGGAGTT+TGG 0.334825 5.4:-47758743 MS.gene010286:CDS
TACCGGACTGAGTAGCAATT+TGG 0.335469 5.4:+47758959 None:intergenic
CAAACAATCACTGTTGTTGA+TGG 0.337185 5.4:-47759056 MS.gene010286:CDS
AACCGCAATTCAACAATGTT+TGG 0.340223 5.4:+47756571 None:intergenic
CAGTGATTGTTTGAGGGTTA+GGG 0.341129 5.4:+47759066 None:intergenic
ATGAAGATTGGTTGGGCATC+TGG 0.343084 5.4:-47758324 MS.gene010286:CDS
ACCTGAAGCATGCGCCAATT+AGG 0.343626 5.4:+47758204 None:intergenic
AGTTCTTGTCCTTCAGTTAC+CGG 0.352672 5.4:+47758942 None:intergenic
CTGGCTCCCTGCATGCCTGC+TGG 0.359242 5.4:+47756543 None:intergenic
TTAGTAGTTTGAACATATTC+GGG 0.360385 5.4:-47756401 MS.gene010286:CDS
GGAGAATTTAAGTCAAGCTT+AGG 0.361917 5.4:+47758792 None:intergenic
AACACAAAAGGAGTTGGTTC+TGG 0.362758 5.4:-47756351 MS.gene010286:CDS
AATTTCATTGGATCGCTTAG+AGG 0.373370 5.4:+47756251 None:intergenic
CCTGAAGCATGCGCCAATTA+GGG 0.379672 5.4:+47758205 None:intergenic
TAAACTTTAGAACGTACCTT+TGG 0.384455 5.4:+47759128 None:intergenic
GAGTCCAACCAGTAGTAAAA+AGG 0.388502 5.4:+47758682 None:intergenic
TCTTTGAATGATAATAGTTA+TGG 0.390167 5.4:-47756486 MS.gene010286:CDS
GGAGTTGGCGGAAAAGAATA+TGG 0.402969 5.4:-47758421 MS.gene010286:CDS
CTCTTTATCATAGGTTAACT+GGG 0.403608 5.4:-47756787 MS.gene010286:intron
GGGACAGACATGGTGGCCGG+TGG 0.403787 5.4:+47759212 None:intergenic
TAGATGCACTGCAGGATTGC+GGG 0.405787 5.4:+47756704 None:intergenic
CAGCAGGCATGCAGGGAGCC+AGG 0.415904 5.4:-47756542 MS.gene010286:CDS
ATGTGTCCAGTGTCCCTAAT+TGG 0.419261 5.4:-47758218 MS.gene010286:CDS
ACTATTATCATTCAAAGAGT+TGG 0.428670 5.4:+47756491 None:intergenic
GGAAATAAGTTCAACTTGCC+AGG 0.428842 5.4:+47756725 None:intergenic
TGCGTCAATAACCGCCTTCA+CGG 0.429525 5.4:+47758446 None:intergenic
GCCGCGTTAAATCCAAAATC+TGG 0.431371 5.4:-47759181 MS.gene010286:CDS
TTTGGTTCAAAGATCTCTTA+GGG 0.434535 5.4:+47756589 None:intergenic
TTGAAGTTATATATTACCCT+AGG 0.447607 5.4:-47758395 MS.gene010286:CDS
CGCCAAATTGCTACTCAGTC+CGG 0.457882 5.4:-47758961 MS.gene010286:CDS
ACAAAATCTGATTCTAATAA+CGG 0.462788 5.4:-47758858 MS.gene010286:CDS
CTTAGAGGAATCTTCTCCCT+TGG 0.463215 5.4:+47756266 None:intergenic
GAACTCTATCATAGGAATGA+AGG 0.464627 5.4:+47756611 None:intergenic
CTCTTAGGGAACTCTATCAT+AGG 0.472447 5.4:+47756603 None:intergenic
ATTGTTTGAGGGTTAGGGGA+AGG 0.473070 5.4:+47759071 None:intergenic
TCAACAACAGTGATTGTTTG+AGG 0.475098 5.4:+47759059 None:intergenic
TTGTTTGAGGGTTAGGGGAA+GGG 0.475352 5.4:+47759072 None:intergenic
GTGGATGATGCTATGAAGAT+TGG 0.483610 5.4:-47758336 MS.gene010286:CDS
CAACAACAGTGATTGTTTGA+GGG 0.485696 5.4:+47759060 None:intergenic
CGAATATGTTCAAACTACTA+AGG 0.486402 5.4:+47756403 None:intergenic
TCTTCTTTATCAACAACCTC+AGG 0.486494 5.4:+47758918 None:intergenic
ACTCACAACAATAACACAAA+AGG 0.489392 5.4:-47756363 MS.gene010286:CDS
TTACTTCCCACAAGGTCACT+TGG 0.494429 5.4:-47759105 MS.gene010286:CDS
TGGAACGTTCACCGATGGAC+CGG 0.495600 5.4:+47759148 None:intergenic
TTGGATTTAACGCGGCGTGC+CGG 0.498738 5.4:+47759188 None:intergenic
ATCAAAACCGCATCAATCCC+TGG 0.500897 5.4:-47756743 MS.gene010286:CDS
GGCCACCATGTCTGTCCCTC+CGG 0.501925 5.4:-47759207 MS.gene010286:CDS
GGTTATTGACGCAGTGGAGT+TGG 0.506246 5.4:-47758436 MS.gene010286:CDS
TTGAGGGTTAGGGGAAGGGC+AGG 0.507377 5.4:+47759076 None:intergenic
TAAAGAAGAAGACGGTGATA+AGG 0.507483 5.4:-47758904 MS.gene010286:CDS
CTCCCTTGGAACCAGAATCA+TGG 0.507619 5.4:+47756280 None:intergenic
GGGGATGAGGGTAAAGAGAA+TGG 0.508401 5.4:-47758522 MS.gene010286:CDS
GATTTAACGCGGCGTGCCGG+AGG 0.509373 5.4:+47759191 None:intergenic
ACCTTTGGAACGTTCACCGA+TGG 0.510835 5.4:+47759143 None:intergenic
TTCCGCCATGTCTTCCCCAC+CGG 0.514610 5.4:-47759228 None:intergenic
AAAATCTGGCAGACATGTGC+CGG 0.515291 5.4:-47759167 MS.gene010286:CDS
TGAGGGTAAAGAGAATGGTT+TGG 0.516087 5.4:-47758517 MS.gene010286:CDS
GGACAGACATGGTGGCCGGT+GGG 0.516458 5.4:+47759213 None:intergenic
GCAGACATGTGCCGGTCCAT+CGG 0.518088 5.4:-47759159 MS.gene010286:CDS
GGTGGCCGGTGGGGAAGACA+TGG 0.519543 5.4:+47759223 None:intergenic
ACTGCCGGAGATATAGTTGT+TGG 0.521728 5.4:-47758603 MS.gene010286:CDS
TCTGATTCTAATAACGGCGG+TGG 0.523119 5.4:-47758852 MS.gene010286:CDS
GAAGATTCATCAATCTTCAA+AGG 0.524456 5.4:+47758285 None:intergenic
GTAGAATCCTGAACTAGTCT+AGG 0.524565 5.4:+47756660 None:intergenic
GAGAGTTCCTGAGACGGAAA+AGG 0.524822 5.4:+47758771 None:intergenic
GAGAATTTAAGTCAAGCTTA+GGG 0.524869 5.4:+47758793 None:intergenic
GACAGACATGGTGGCCGGTG+GGG 0.526151 5.4:+47759214 None:intergenic
GACCTCATCGGTGTGAGGAT+CGG 0.532501 5.4:+47758986 None:intergenic
AACAATAACACAAAAGGAGT+TGG 0.535208 5.4:-47756357 MS.gene010286:CDS
GGAGGGACAGACATGGTGGC+CGG 0.536529 5.4:+47759209 None:intergenic
AACCAAACATTGTTGAATTG+CGG 0.536631 5.4:-47756573 MS.gene010286:CDS
GGTTTGGAGGTCAAAGGAGA+GGG 0.536796 5.4:-47758501 MS.gene010286:CDS
AACTCTATCATAGGAATGAA+GGG 0.541293 5.4:+47756612 None:intergenic
AGAAATGGTAGATGCACTGC+AGG 0.541883 5.4:+47756696 None:intergenic
GATTCCAACAACTATATCTC+CGG 0.544415 5.4:+47758599 None:intergenic
CATGTTCCAAGTGACCTTGT+GGG 0.545385 5.4:+47759099 None:intergenic
TACCTTGTTTAGTGATAACA+AGG 0.546334 5.4:-47756640 MS.gene010286:CDS
TATTGACGCAGTGGAGTTGG+CGG 0.549772 5.4:-47758433 MS.gene010286:CDS
TGGTTTGGAGGTCAAAGGAG+AGG 0.550776 5.4:-47758502 MS.gene010286:CDS
TAGAGATAAATGATCATGCC+TGG 0.553837 5.4:+47756524 None:intergenic
GTCCATGATTCTGGTTCCAA+GGG 0.555126 5.4:-47756282 MS.gene010286:CDS
TGTCCATGATTCTGGTTCCA+AGG 0.556510 5.4:-47756283 MS.gene010286:CDS
TCAACGGCCGTGATGATACA+AGG 0.557986 5.4:+47759017 None:intergenic
ATGATGCTATGAAGATTGGT+TGG 0.562627 5.4:-47758332 MS.gene010286:CDS
GAGAATGGTTTGGAGGTCAA+AGG 0.563275 5.4:-47758507 MS.gene010286:CDS
TGAAGTTATATATTACCCTA+GGG 0.565837 5.4:-47758394 MS.gene010286:CDS
GTTTACTGCAGCTGAATCAA+AGG 0.566092 5.4:-47758562 MS.gene010286:CDS
TGGCGACAAGTCGTTAGATG+TGG 0.568778 5.4:+47756431 None:intergenic
GCATGTTCCAAGTGACCTTG+TGG 0.569473 5.4:+47759098 None:intergenic
GGGTAAAGAGAATGGTTTGG+AGG 0.569581 5.4:-47758514 MS.gene010286:CDS
AAATTGCACCAATTAGCCCT+AGG 0.571867 5.4:+47758378 None:intergenic
CAGTAAACTTCATGTTTCTG+CGG 0.572135 5.4:+47758577 None:intergenic
ATCATGGACATCACTTTCAA+CGG 0.572291 5.4:+47756296 None:intergenic
GTTGTTGATAAAGAAGAAGA+CGG 0.573045 5.4:-47758912 MS.gene010286:CDS
TGTAGTAGATGCAAATGTTG+TGG 0.577875 5.4:-47758355 MS.gene010286:CDS
CTACTCAGTCCGGTAACTGA+AGG 0.578098 5.4:-47758951 MS.gene010286:CDS
TGATGCTATGAAGATTGGTT+GGG 0.580467 5.4:-47758331 MS.gene010286:CDS
AAAGGCAGTGAATAATAGGA+AGG 0.580552 5.4:-47758544 MS.gene010286:CDS
GAAATAAGTTCAACTTGCCA+GGG 0.590783 5.4:+47756726 None:intergenic
GTAGATGCACTGCAGGATTG+CGG 0.593419 5.4:+47756703 None:intergenic
GTGATGATACAAGGAAAAGA+AGG 0.595656 5.4:+47759026 None:intergenic
AGTGATTGTTTGAGGGTTAG+GGG 0.595931 5.4:+47759067 None:intergenic
ATTTAACGCGGCGTGCCGGA+GGG 0.596031 5.4:+47759192 None:intergenic
GGCGACAAGTCGTTAGATGT+GGG 0.596585 5.4:+47756432 None:intergenic
CATGCGCCAATTAGGGACAC+TGG 0.600814 5.4:+47758212 None:intergenic
AGGCAGTGAATAATAGGAAG+GGG 0.602083 5.4:-47758542 MS.gene010286:CDS
TCCATCGGTGAACGTTCCAA+AGG 0.605082 5.4:-47759144 MS.gene010286:CDS
CAACTTGCCAGGGATTGATG+CGG 0.611590 5.4:+47756736 None:intergenic
AAGTTTGCTCATGTCTATCG+AGG 0.613801 5.4:-47758720 MS.gene010286:CDS
AAGGCAGTGAATAATAGGAA+GGG 0.619996 5.4:-47758543 MS.gene010286:CDS
ACCCTTGTTATCACTAAACA+AGG 0.625546 5.4:+47756638 None:intergenic
CAATTTGGCGAAGACCTCAT+CGG 0.632488 5.4:+47758974 None:intergenic
ATCAAGTCAGCACAAAGGCG+AGG 0.638663 5.4:+47758819 None:intergenic
AATTGCACCAATTAGCCCTA+GGG 0.639117 5.4:+47758379 None:intergenic
GCGTGCCGGAGGGACAGACA+TGG 0.644657 5.4:+47759202 None:intergenic
AAGAGGAATACTAACCGTGA+AGG 0.646055 5.4:-47758460 MS.gene010286:CDS
TCCGTCATGAACATCAGTAA+CGG 0.649838 5.4:+47758749 None:intergenic
GCGAAGACCTCATCGGTGTG+AGG 0.652471 5.4:+47758981 None:intergenic
AGTTTGCTCATGTCTATCGA+GGG 0.654001 5.4:-47758719 MS.gene010286:CDS
TCCGTTACTGATGTTCATGA+CGG 0.654864 5.4:-47758750 MS.gene010286:CDS
CATCGGTGTGAGGATCGGCT+AGG 0.655915 5.4:+47758991 None:intergenic
AGGAATACTAACCGTGAAGG+CGG 0.658064 5.4:-47758457 MS.gene010286:CDS
AGCCGATCCTCACACCGATG+AGG 0.659256 5.4:-47758988 MS.gene010286:CDS
AAAGTTTATTACTTCCCACA+AGG 0.663461 5.4:-47759113 MS.gene010286:CDS
GAAGGCGGTTATTGACGCAG+TGG 0.665181 5.4:-47758442 MS.gene010286:CDS
ACATTAGAGATTGTTCCTTG+AGG 0.666803 5.4:+47758237 None:intergenic
GGCAGTGAATAATAGGAAGG+GGG 0.673747 5.4:-47758541 MS.gene010286:CDS
TGATACAAGGAAAAGAAGGA+CGG 0.677808 5.4:+47759030 None:intergenic
TGCCGGAGGGACAGACATGG+TGG 0.679386 5.4:+47759205 None:intergenic
TGAAGGACAAGAACTTCCTG+AGG 0.686819 5.4:-47758934 MS.gene010286:CDS
GTAACGGAGAGTTCCTGAGA+CGG 0.687969 5.4:+47758765 None:intergenic
AGGATCGGCTAGGAGATCAA+CGG 0.692255 5.4:+47759001 None:intergenic
GGAAAATCAAGTCAGCACAA+AGG 0.697888 5.4:+47758814 None:intergenic
AAATCTGATTCTAATAACGG+CGG 0.706742 5.4:-47758855 MS.gene010286:CDS
ACCTTGTTTAGTGATAACAA+GGG 0.724822 5.4:-47756639 MS.gene010286:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAGTATACAAATATTA+GGG - chr5.4:47758026-47758045 MS.gene010286:intron 10.0%
!! TAATATTACAAAAAATTAGA+AGG - chr5.4:47757551-47757570 MS.gene010286:intron 10.0%
!!! TATTTTATTTTTTGAATCAA+GGG - chr5.4:47757317-47757336 MS.gene010286:intron 10.0%
!!! TGCATTTTATTATAAAAATA+TGG + chr5.4:47758527-47758546 None:intergenic 10.0%
!!! TTATTTTATTTTTTGAATCA+AGG - chr5.4:47757316-47757335 MS.gene010286:intron 10.0%
!! ATAGTTTAGTATACTTAATT+AGG + chr5.4:47757529-47757548 None:intergenic 15.0%
!! CAATTTAGTATACAAATATT+AGG - chr5.4:47758025-47758044 MS.gene010286:intron 15.0%
!! TATATATAACTTATCCTATA+GGG + chr5.4:47758100-47758119 None:intergenic 15.0%
!! TTATATATAACTTATCCTAT+AGG + chr5.4:47758101-47758120 None:intergenic 15.0%
!!! ATATTTTAAATGACTGATAT+TGG - chr5.4:47758642-47758661 MS.gene010286:CDS 15.0%
!! ACAAAATCTGATTCTAATAA+CGG - chr5.4:47756611-47756630 MS.gene010286:CDS 20.0%
!! CTATGATAAAGAGATAAAAA+TGG + chr5.4:47758675-47758694 None:intergenic 20.0%
!! TCTTTGAATGATAATAGTTA+TGG - chr5.4:47758983-47759002 MS.gene010286:CDS 20.0%
!! TTGTATACTAAATTGATCAA+GGG + chr5.4:47758020-47758039 None:intergenic 20.0%
!! TTTGTATACTAAATTGATCA+AGG + chr5.4:47758021-47758040 None:intergenic 20.0%
!!! CATTTTTATCTCTTTATCAT+AGG - chr5.4:47758673-47758692 MS.gene010286:CDS 20.0%
!!! TGGAAAAGTATTAGTTATTT+AGG - chr5.4:47758498-47758517 MS.gene010286:CDS 20.0%
! AAAACATGACTCTTTAAACT+TGG + chr5.4:47757995-47758014 None:intergenic 25.0%
! AAATTAACGACTAACTTAGT+TGG + chr5.4:47758142-47758161 None:intergenic 25.0%
! ACTATTATCATTCAAAGAGT+TGG + chr5.4:47758981-47759000 None:intergenic 25.0%
! AGTATACAAATATTAGGGTT+AGG - chr5.4:47758031-47758050 MS.gene010286:intron 25.0%
! ATAAATAATGAAGAAGCTTC+AGG - chr5.4:47758468-47758487 MS.gene010286:CDS 25.0%
! ATCAATCAATCAATCATATG+AGG + chr5.4:47758408-47758427 None:intergenic 25.0%
! ATGATTACCAAAATACAGTT+AGG - chr5.4:47757680-47757699 MS.gene010286:intron 25.0%
! CCAAAATCAATCCATAATTA+TGG - chr5.4:47758305-47758324 MS.gene010286:CDS 25.0%
! CTTAGTAGTTTGAACATATT+CGG - chr5.4:47759067-47759086 MS.gene010286:CDS 25.0%
! GTAAAATATAGATTCTCCTT+AGG + chr5.4:47758562-47758581 None:intergenic 25.0%
! TATTATTGTCGAAGAAGATT+AGG - chr5.4:47757371-47757390 MS.gene010286:intron 25.0%
! TGAAGTTATATATTACCCTA+GGG - chr5.4:47757075-47757094 MS.gene010286:intron 25.0%
! TTAGTAGTTTGAACATATTC+GGG - chr5.4:47759068-47759087 MS.gene010286:CDS 25.0%
! TTCAAACTACTAAGGTTATT+TGG + chr5.4:47759061-47759080 None:intergenic 25.0%
! TTGAAGTTATATATTACCCT+AGG - chr5.4:47757074-47757093 MS.gene010286:intron 25.0%
!! GATTTTTCCTAACTGTATTT+TGG + chr5.4:47757690-47757709 None:intergenic 25.0%
!! TCTGGTTCAATTAAGTTATT+TGG - chr5.4:47759136-47759155 MS.gene010286:CDS 25.0%
!! TTGTGATTTTTCCTGTATTT+TGG + chr5.4:47757789-47757808 None:intergenic 25.0%
!!! CCATAATTATGGATTGATTT+TGG + chr5.4:47758308-47758327 None:intergenic 25.0%
!!! GTCGTTAATTTACTGTTTTT+TGG - chr5.4:47758151-47758170 MS.gene010286:intron 25.0%
AAAGTTTATTACTTCCCACA+AGG - chr5.4:47756356-47756375 MS.gene010286:CDS 30.0%
AAATATTAGGGTTAGGTGAT+AGG - chr5.4:47758038-47758057 MS.gene010286:intron 30.0%
AAATCTGATTCTAATAACGG+CGG - chr5.4:47756614-47756633 MS.gene010286:CDS 30.0%
AACAATAACACAAAAGGAGT+TGG - chr5.4:47759112-47759131 MS.gene010286:CDS 30.0%
AACCAAACATTGTTGAATTG+CGG - chr5.4:47758896-47758915 MS.gene010286:CDS 30.0%
AACTCTATCATAGGAATGAA+GGG + chr5.4:47758860-47758879 None:intergenic 30.0%
AATCAAAGGCAGTGAATAAT+AGG - chr5.4:47756921-47756940 MS.gene010286:intron 30.0%
ACTCACAACAATAACACAAA+AGG - chr5.4:47759106-47759125 MS.gene010286:CDS 30.0%
AGACATGATTACCAAAATAC+AGG - chr5.4:47757676-47757695 MS.gene010286:intron 30.0%
CATGATCATTTATCTCTATC+AGG - chr5.4:47758950-47758969 MS.gene010286:CDS 30.0%
CGAATATGTTCAAACTACTA+AGG + chr5.4:47759069-47759088 None:intergenic 30.0%
CTCTTTATCATAGGTTAACT+GGG - chr5.4:47758682-47758701 MS.gene010286:CDS 30.0%
GAAGATTCATCAATCTTCAA+AGG + chr5.4:47757187-47757206 None:intergenic 30.0%
GAGAATTTAAGTCAAGCTTA+GGG + chr5.4:47756679-47756698 None:intergenic 30.0%
GAGTGAAGATATAGATAACT+TGG - chr5.4:47757921-47757940 MS.gene010286:intron 30.0%
TCTCTTTATCATAGGTTAAC+TGG - chr5.4:47758681-47758700 MS.gene010286:CDS 30.0%
TTGCAGTTGATCCATAATTA+TGG + chr5.4:47758319-47758338 None:intergenic 30.0%
! AAAAAAAGGTTTTCGGAAGT+GGG + chr5.4:47757607-47757626 None:intergenic 30.0%
! AAAAGACACCTTTTTACTAC+TGG - chr5.4:47756779-47756798 MS.gene010286:intron 30.0%
! AAAAGAGTGACAAGTGTTTT+CGG - chr5.4:47758441-47758460 MS.gene010286:CDS 30.0%
! ACCTTGTTTAGTGATAACAA+GGG - chr5.4:47758830-47758849 MS.gene010286:CDS 30.0%
! GAGCTGTAAAAAAAGGTTTT+CGG + chr5.4:47757614-47757633 None:intergenic 30.0%
! GTTGAATAATGTTTTCAGCA+AGG + chr5.4:47757649-47757668 None:intergenic 30.0%
! GTTGTTGATAAAGAAGAAGA+CGG - chr5.4:47756557-47756576 MS.gene010286:CDS 30.0%
! TAAAAAAAGGTTTTCGGAAG+TGG + chr5.4:47757608-47757627 None:intergenic 30.0%
! TACCTTGTTTAGTGATAACA+AGG - chr5.4:47758829-47758848 MS.gene010286:CDS 30.0%
! TTCTTTTCCTTGTATCATCA+CGG - chr5.4:47756445-47756464 MS.gene010286:CDS 30.0%
! TTTGGTTCAAAGATCTCTTA+GGG + chr5.4:47758883-47758902 None:intergenic 30.0%
! TTTTGTAAGAGTCTTTACGA+AGG + chr5.4:47756597-47756616 None:intergenic 30.0%
!! TAAACTTTAGAACGTACCTT+TGG + chr5.4:47756344-47756363 None:intergenic 30.0%
!!! AGTAGTAAAAAGGTGTCTTT+TGG + chr5.4:47756780-47756799 None:intergenic 30.0%
!!! AGTTTTTTCGTGTTCACAAA+CGG + chr5.4:47756812-47756831 None:intergenic 30.0%
!!! GTAGTAAAAAGGTGTCTTTT+GGG + chr5.4:47756779-47756798 None:intergenic 30.0%
AAAGGCAGTGAATAATAGGA+AGG - chr5.4:47756925-47756944 MS.gene010286:intron 35.0%
AACCGCAATTCAACAATGTT+TGG + chr5.4:47758901-47758920 None:intergenic 35.0%
AAGAAGCTTCAGGTATCTTA+TGG - chr5.4:47758478-47758497 MS.gene010286:CDS 35.0%
AAGGCAGTGAATAATAGGAA+GGG - chr5.4:47756926-47756945 MS.gene010286:intron 35.0%
AATCATATGAGGAGCAAGAA+GGG + chr5.4:47758397-47758416 None:intergenic 35.0%
AATTTCATTGGATCGCTTAG+AGG + chr5.4:47759221-47759240 None:intergenic 35.0%
ACATTAGAGATTGTTCCTTG+AGG + chr5.4:47757235-47757254 None:intergenic 35.0%
ATCATGGACATCACTTTCAA+CGG + chr5.4:47759176-47759195 None:intergenic 35.0%
ATGATGCTATGAAGATTGGT+TGG - chr5.4:47757137-47757156 MS.gene010286:intron 35.0%
CAAACAATCACTGTTGTTGA+TGG - chr5.4:47756413-47756432 MS.gene010286:CDS 35.0%
CAACAACAGTGATTGTTTGA+GGG + chr5.4:47756412-47756431 None:intergenic 35.0%
CAATACTATGAATCGATGCA+AGG + chr5.4:47757500-47757519 None:intergenic 35.0%
GAAATAAGTTCAACTTGCCA+GGG + chr5.4:47758746-47758765 None:intergenic 35.0%
GAACTCTATCATAGGAATGA+AGG + chr5.4:47758861-47758880 None:intergenic 35.0%
GATTCCAACAACTATATCTC+CGG + chr5.4:47756873-47756892 None:intergenic 35.0%
GGAGAATTTAAGTCAAGCTT+AGG + chr5.4:47756680-47756699 None:intergenic 35.0%
GGTATATATGTTTCAGCAAG+AGG - chr5.4:47757896-47757915 MS.gene010286:intron 35.0%
GTGATGATACAAGGAAAAGA+AGG + chr5.4:47756446-47756465 None:intergenic 35.0%
GTTGGTTGAGCTGTAAAAAA+AGG + chr5.4:47757621-47757640 None:intergenic 35.0%
TAAAGAAGAAGACGGTGATA+AGG - chr5.4:47756565-47756584 MS.gene010286:CDS 35.0%
TAGAGATAAATGATCATGCC+TGG + chr5.4:47758948-47758967 None:intergenic 35.0%
TATATTACCCTAGGGCTAAT+TGG - chr5.4:47757083-47757102 MS.gene010286:intron 35.0%
TCAACAACAGTGATTGTTTG+AGG + chr5.4:47756413-47756432 None:intergenic 35.0%
TCTTCTTTATCAACAACCTC+AGG + chr5.4:47756554-47756573 None:intergenic 35.0%
TGAGAAGAATTCGATGCATA+CGG - chr5.4:47757465-47757484 MS.gene010286:intron 35.0%
TGATACAAGGAAAAGAAGGA+CGG + chr5.4:47756442-47756461 None:intergenic 35.0%
TGCATTTGCTCTTATTCCTA+AGG - chr5.4:47758543-47758562 MS.gene010286:CDS 35.0%
TGTAGTAGATGCAAATGTTG+TGG - chr5.4:47757114-47757133 MS.gene010286:intron 35.0%
! AAAAAAGCCTAGACTAGTTC+AGG - chr5.4:47758802-47758821 MS.gene010286:CDS 35.0%
! AAAGAGTGACAAGTGTTTTC+GGG - chr5.4:47758442-47758461 MS.gene010286:CDS 35.0%
! ACCCTTGTTATCACTAAACA+AGG + chr5.4:47758834-47758853 None:intergenic 35.0%
! CAGTAAACTTCATGTTTCTG+CGG + chr5.4:47756895-47756914 None:intergenic 35.0%
! GTTTGGTTCAAAGATCTCTT+AGG + chr5.4:47758884-47758903 None:intergenic 35.0%
! GTTTTCATGAAAAACACTGC+CGG - chr5.4:47756851-47756870 MS.gene010286:intron 35.0%
! TGATGCTATGAAGATTGGTT+GGG - chr5.4:47757138-47757157 MS.gene010286:intron 35.0%
!!! ATGACATTTTTTCAGCCTCA+AGG - chr5.4:47757217-47757236 MS.gene010286:intron 35.0%
!!! GGCTTTTTTGTTGAGAGAAA+TGG + chr5.4:47758791-47758810 None:intergenic 35.0%
AAATTGCACCAATTAGCCCT+AGG + chr5.4:47757094-47757113 None:intergenic 40.0%
AACAATGATCAAGGCTCTCA+GGG + chr5.4:47757969-47757988 None:intergenic 40.0%
AACACGAAAAAACTCGTTGC+TGG - chr5.4:47756818-47756837 MS.gene010286:intron 40.0%
AAGAGGAATACTAACCGTGA+AGG - chr5.4:47757009-47757028 MS.gene010286:intron 40.0%
AATAATAGGAAGGGGGATGA+GGG - chr5.4:47756935-47756954 MS.gene010286:intron 40.0%
AATTGCACCAATTAGCCCTA+GGG + chr5.4:47757093-47757112 None:intergenic 40.0%
ACAGTGATTGTTTGAGGGTT+AGG + chr5.4:47756407-47756426 None:intergenic 40.0%
ACTTGGAGCAACAATGATCA+AGG + chr5.4:47757978-47757997 None:intergenic 40.0%
AGGCAGTGAATAATAGGAAG+GGG - chr5.4:47756927-47756946 MS.gene010286:intron 40.0%
AGTGATTGTTTGAGGGTTAG+GGG + chr5.4:47756405-47756424 None:intergenic 40.0%
AGTTCTTGTCCTTCAGTTAC+CGG + chr5.4:47756530-47756549 None:intergenic 40.0%
ATTCGCATTTCGCGTTGTTA+CGG - chr5.4:47757425-47757444 MS.gene010286:intron 40.0%
CAATCATATGAGGAGCAAGA+AGG + chr5.4:47758398-47758417 None:intergenic 40.0%
CAGTGATTGTTTGAGGGTTA+GGG + chr5.4:47756406-47756425 None:intergenic 40.0%
CTCTTAGGGAACTCTATCAT+AGG + chr5.4:47758869-47758888 None:intergenic 40.0%
GAAAGTGATGTCCATGATTC+TGG - chr5.4:47759178-47759197 MS.gene010286:CDS 40.0%
GAGTCCAACCAGTAGTAAAA+AGG + chr5.4:47756790-47756809 None:intergenic 40.0%
GGAAAATCAAGTCAGCACAA+AGG + chr5.4:47756658-47756677 None:intergenic 40.0%
GGAAATAAGTTCAACTTGCC+AGG + chr5.4:47758747-47758766 None:intergenic 40.0%
GTGGATGATGCTATGAAGAT+TGG - chr5.4:47757133-47757152 MS.gene010286:intron 40.0%
GTTTACTGCAGCTGAATCAA+AGG - chr5.4:47756907-47756926 MS.gene010286:intron 40.0%
TCCGTCATGAACATCAGTAA+CGG + chr5.4:47756723-47756742 None:intergenic 40.0%
TCCGTTACTGATGTTCATGA+CGG - chr5.4:47756719-47756738 MS.gene010286:CDS 40.0%
TGAGGGTAAAGAGAATGGTT+TGG - chr5.4:47756952-47756971 MS.gene010286:intron 40.0%
! AAATTCTCCTTTTCCGTCTC+AGG - chr5.4:47756691-47756710 MS.gene010286:CDS 40.0%
! AACACAAAAGGAGTTGGTTC+TGG - chr5.4:47759118-47759137 MS.gene010286:CDS 40.0%
! AAGTTTGCTCATGTCTATCG+AGG - chr5.4:47756749-47756768 MS.gene010286:CDS 40.0%
! AGTTTGCTCATGTCTATCGA+GGG - chr5.4:47756750-47756769 MS.gene010286:CDS 40.0%
! GACACCTTTTTACTACTGGT+TGG - chr5.4:47756783-47756802 MS.gene010286:intron 40.0%
! GCCAGATTTTGGATTTAACG+CGG + chr5.4:47756292-47756311 None:intergenic 40.0%
! GTAGAATCCTGAACTAGTCT+AGG + chr5.4:47758812-47758831 None:intergenic 40.0%
! GTTTTCAGCAAGGATTGTGT+TGG + chr5.4:47757639-47757658 None:intergenic 40.0%
!! AATTGCGGTTATTTTCCAGC+AGG - chr5.4:47758911-47758930 MS.gene010286:CDS 40.0%
!! ATAGCTTTGTTGTGCCCTAT+AGG - chr5.4:47758083-47758102 MS.gene010286:intron 40.0%
!!! CTTCAGGTGCTCTTCTTTTT+TGG - chr5.4:47757280-47757299 MS.gene010286:intron 40.0%
AAAATCTGGCAGACATGTGC+CGG - chr5.4:47756302-47756321 MS.gene010286:CDS 45.0%
ACTGCCGGAGATATAGTTGT+TGG - chr5.4:47756866-47756885 MS.gene010286:intron 45.0%
AGAAATGGTAGATGCACTGC+AGG + chr5.4:47758776-47758795 None:intergenic 45.0%
AGGAATACTAACCGTGAAGG+CGG - chr5.4:47757012-47757031 MS.gene010286:intron 45.0%
ATCAAAACCGCATCAATCCC+TGG - chr5.4:47758726-47758745 MS.gene010286:CDS 45.0%
ATGTGTCCAGTGTCCCTAAT+TGG - chr5.4:47757251-47757270 MS.gene010286:intron 45.0%
ATTGTTTGAGGGTTAGGGGA+AGG + chr5.4:47756401-47756420 None:intergenic 45.0%
CAACAATGATCAAGGCTCTC+AGG + chr5.4:47757970-47757989 None:intergenic 45.0%
CAATTTGGCGAAGACCTCAT+CGG + chr5.4:47756498-47756517 None:intergenic 45.0%
CATGTTCCAAGTGACCTTGT+GGG + chr5.4:47756373-47756392 None:intergenic 45.0%
CTTAGAGGAATCTTCTCCCT+TGG + chr5.4:47759206-47759225 None:intergenic 45.0%
GAAGAATTCGATGCATACGG+AGG - chr5.4:47757468-47757487 MS.gene010286:intron 45.0%
GAATAATAGGAAGGGGGATG+AGG - chr5.4:47756934-47756953 MS.gene010286:intron 45.0%
GCCGCGTTAAATCCAAAATC+TGG - chr5.4:47756288-47756307 MS.gene010286:CDS 45.0%
GGAGTTGGCGGAAAAGAATA+TGG - chr5.4:47757048-47757067 MS.gene010286:intron 45.0%
GGCAGTGAATAATAGGAAGG+GGG - chr5.4:47756928-47756947 MS.gene010286:intron 45.0%
TACCGGACTGAGTAGCAATT+TGG + chr5.4:47756513-47756532 None:intergenic 45.0%
TCTGATTCTAATAACGGCGG+TGG - chr5.4:47756617-47756636 MS.gene010286:CDS 45.0%
TGAAGGACAAGAACTTCCTG+AGG - chr5.4:47756535-47756554 MS.gene010286:CDS 45.0%
TTACTTCCCACAAGGTCACT+TGG - chr5.4:47756364-47756383 MS.gene010286:CDS 45.0%
TTCGCATTTCGCGTTGTTAC+GGG - chr5.4:47757426-47757445 MS.gene010286:intron 45.0%
TTGTTTGAGGGTTAGGGGAA+GGG + chr5.4:47756400-47756419 None:intergenic 45.0%
! AAAGGAGAGGGTTTTTCTCG+AGG - chr5.4:47756980-47756999 MS.gene010286:intron 45.0%
! ATGAAGATTGGTTGGGCATC+TGG - chr5.4:47757145-47757164 MS.gene010286:intron 45.0%
! CTGATGTTCATGACGGAGTT+TGG - chr5.4:47756726-47756745 MS.gene010286:CDS 45.0%
! GAGAATGGTTTGGAGGTCAA+AGG - chr5.4:47756962-47756981 MS.gene010286:intron 45.0%
! GGGTAAAGAGAATGGTTTGG+AGG - chr5.4:47756955-47756974 MS.gene010286:intron 45.0%
! TATTTTCCAGCAGGCATGCA+GGG - chr5.4:47758920-47758939 MS.gene010286:CDS 45.0%
! TTATTTTCCAGCAGGCATGC+AGG - chr5.4:47758919-47758938 MS.gene010286:CDS 45.0%
!! GTCCATGATTCTGGTTCCAA+GGG - chr5.4:47759187-47759206 MS.gene010286:CDS 45.0%
!! TGTCCATGATTCTGGTTCCA+AGG - chr5.4:47759186-47759205 MS.gene010286:CDS 45.0%
ACCTGAAGCATGCGCCAATT+AGG + chr5.4:47757268-47757287 None:intergenic 50.0%
AGGATCGGCTAGGAGATCAA+CGG + chr5.4:47756471-47756490 None:intergenic 50.0%
ATCAAGTCAGCACAAAGGCG+AGG + chr5.4:47756653-47756672 None:intergenic 50.0%
CAACTTGCCAGGGATTGATG+CGG + chr5.4:47758736-47758755 None:intergenic 50.0%
CACAATAGCAGCAGCAACAG+TGG - chr5.4:47757875-47757894 MS.gene010286:intron 50.0%
CACTGTTGCTGCTGCTATTG+TGG + chr5.4:47757877-47757896 None:intergenic 50.0%
CCTGAAGCATGCGCCAATTA+GGG + chr5.4:47757267-47757286 None:intergenic 50.0%
CGCCAAATTGCTACTCAGTC+CGG - chr5.4:47756508-47756527 MS.gene010286:CDS 50.0%
CTACTCAGTCCGGTAACTGA+AGG - chr5.4:47756518-47756537 MS.gene010286:CDS 50.0%
CTCCCTTGGAACCAGAATCA+TGG + chr5.4:47759192-47759211 None:intergenic 50.0%
GAGAGTTCCTGAGACGGAAA+AGG + chr5.4:47756701-47756720 None:intergenic 50.0%
GCATGTTCCAAGTGACCTTG+TGG + chr5.4:47756374-47756393 None:intergenic 50.0%
GGCGACAAGTCGTTAGATGT+GGG + chr5.4:47759040-47759059 None:intergenic 50.0%
GGGGATGAGGGTAAAGAGAA+TGG - chr5.4:47756947-47756966 MS.gene010286:intron 50.0%
GGTTATTGACGCAGTGGAGT+TGG - chr5.4:47757033-47757052 MS.gene010286:intron 50.0%
GGTTTGGAGGTCAAAGGAGA+GGG - chr5.4:47756968-47756987 MS.gene010286:intron 50.0%
GTAGATGCACTGCAGGATTG+CGG + chr5.4:47758769-47758788 None:intergenic 50.0%
TAGATGCACTGCAGGATTGC+GGG + chr5.4:47758768-47758787 None:intergenic 50.0%
TATTGACGCAGTGGAGTTGG+CGG - chr5.4:47757036-47757055 MS.gene010286:intron 50.0%
TCAACGGCCGTGATGATACA+AGG + chr5.4:47756455-47756474 None:intergenic 50.0%
TCCATCGGTGAACGTTCCAA+AGG - chr5.4:47756325-47756344 MS.gene010286:CDS 50.0%
TGCGTCAATAACCGCCTTCA+CGG + chr5.4:47757026-47757045 None:intergenic 50.0%
TGGCGACAAGTCGTTAGATG+TGG + chr5.4:47759041-47759060 None:intergenic 50.0%
TTCGCGTTGTTACGGGTTTG+CGG - chr5.4:47757433-47757452 MS.gene010286:intron 50.0%
! GGCACATGTCTGCCAGATTT+TGG + chr5.4:47756303-47756322 None:intergenic 50.0%
! GGGTTAGGTGATAGGAGTGT+AGG - chr5.4:47758046-47758065 MS.gene010286:intron 50.0%
! GTAACGGAGAGTTCCTGAGA+CGG + chr5.4:47756707-47756726 None:intergenic 50.0%
! TGGTTTGGAGGTCAAAGGAG+AGG - chr5.4:47756967-47756986 MS.gene010286:intron 50.0%
!! ACCTTTGGAACGTTCACCGA+TGG + chr5.4:47756329-47756348 None:intergenic 50.0%
CATGCGCCAATTAGGGACAC+TGG + chr5.4:47757260-47757279 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTC+AGG - chr5.4:47757264-47757283 MS.gene010286:intron 55.0%
GAAGGCGGTTATTGACGCAG+TGG - chr5.4:47757027-47757046 MS.gene010286:intron 55.0%
GACCTCATCGGTGTGAGGAT+CGG + chr5.4:47756486-47756505 None:intergenic 55.0%
TGGAACGTTCACCGATGGAC+CGG + chr5.4:47756324-47756343 None:intergenic 55.0%
TTGGATTTAACGCGGCGTGC+CGG + chr5.4:47756284-47756303 None:intergenic 55.0%
! GAGAGGGTTTTTCTCGAGGC+GGG - chr5.4:47756984-47757003 MS.gene010286:intron 55.0%
! GGAGAGGGTTTTTCTCGAGG+CGG - chr5.4:47756983-47757002 MS.gene010286:intron 55.0%
! TGAGGAGCAAGAAGGGTGCA+TGG + chr5.4:47758390-47758409 None:intergenic 55.0%
! TTTTCTCGAGGCGGGAGAAG+AGG - chr5.4:47756992-47757011 MS.gene010286:intron 55.0%
AGCCGATCCTCACACCGATG+AGG - chr5.4:47756481-47756500 MS.gene010286:CDS 60.0%
ATTTAACGCGGCGTGCCGGA+GGG + chr5.4:47756280-47756299 None:intergenic 60.0%
CATCGGTGTGAGGATCGGCT+AGG + chr5.4:47756481-47756500 None:intergenic 60.0%
GCGAAGACCTCATCGGTGTG+AGG + chr5.4:47756491-47756510 None:intergenic 60.0%
TTGAGGGTTAGGGGAAGGGC+AGG + chr5.4:47756396-47756415 None:intergenic 60.0%
! GCAGACATGTGCCGGTCCAT+CGG - chr5.4:47756310-47756329 MS.gene010286:CDS 60.0%
GACAGACATGGTGGCCGGTG+GGG + chr5.4:47756258-47756277 None:intergenic 65.0%
GATTTAACGCGGCGTGCCGG+AGG + chr5.4:47756281-47756300 None:intergenic 65.0%
GGACAGACATGGTGGCCGGT+GGG + chr5.4:47756259-47756278 None:intergenic 65.0%
GGAGGGACAGACATGGTGGC+CGG + chr5.4:47756263-47756282 None:intergenic 65.0%
GGCCACCATGTCTGTCCCTC+CGG - chr5.4:47756262-47756281 MS.gene010286:CDS 65.0%
TGCCGGAGGGACAGACATGG+TGG + chr5.4:47756267-47756286 None:intergenic 65.0%
CAGCAGGCATGCAGGGAGCC+AGG - chr5.4:47758927-47758946 MS.gene010286:CDS 70.0%
CTGGCTCCCTGCATGCCTGC+TGG + chr5.4:47758929-47758948 None:intergenic 70.0%
GCGTGCCGGAGGGACAGACA+TGG + chr5.4:47756270-47756289 None:intergenic 70.0%
GGGACAGACATGGTGGCCGG+TGG + chr5.4:47756260-47756279 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 47756248 47759243 47756248 ID=MS.gene010286
chr5.4 mRNA 47756248 47759243 47756248 ID=MS.gene010286.t1;Parent=MS.gene010286
chr5.4 exon 47758206 47759243 47758206 ID=MS.gene010286.t1.exon1;Parent=MS.gene010286.t1
chr5.4 CDS 47758206 47759243 47758206 ID=cds.MS.gene010286.t1;Parent=MS.gene010286.t1
chr5.4 exon 47756248 47756796 47756248 ID=MS.gene010286.t1.exon2;Parent=MS.gene010286.t1
chr5.4 CDS 47756248 47756796 47756248 ID=cds.MS.gene010286.t1;Parent=MS.gene010286.t1
Gene Sequence

>MS.gene010286

ATGTCTTCCCCACCGGCCACCATGTCTGTCCCTCCGGCACGCCGCGTTAAATCCAAAATCTGGCAGACATGTGCCGGTCCATCGGTGAACGTTCCAAAGGTACGTTCTAAAGTTTATTACTTCCCACAAGGTCACTTGGAACATGCCTGCCCTTCCCCTAACCCTCAAACAATCACTGTTGTTGATGGTTACCGTCCTTCTTTTCCTTGTATCATCACGGCCGTTGATCTCCTAGCCGATCCTCACACCGATGAGGTCTTCGCCAAATTGCTACTCAGTCCGGTAACTGAAGGACAAGAACTTCCTGAGGTTGTTGATAAAGAAGAAGACGGTGATAAGGTTGTTTCCTTCGTAAAGACTCTTACAAAATCTGATTCTAATAACGGCGGTGGATTCTCTGTACCTCGCCTTTGTGCTGACTTGATTTTCCCTAAGCTTGACTTAAATTCTCCTTTTCCGTCTCAGGAACTCTCCGTTACTGATGTTCATGACGGAGTTTGGAAGTTTGCTCATGTCTATCGAGGGAGACCCAAAAGACACCTTTTTACTACTGGTTGGACTCCGTTTGTGAACACGAAAAAACTCGTTGCTGGTGATTCTATTGTTTTCATGAAAAACACTGCCGGAGATATAGTTGTTGGAATCCGCAGAAACATGAAGTTTACTGCAGCTGAATCAAAGGCAGTGAATAATAGGAAGGGGGATGAGGGTAAAGAGAATGGTTTGGAGGTCAAAGGAGAGGGTTTTTCTCGAGGCGGGAGAAGAGGAATACTAACCGTGAAGGCGGTTATTGACGCAGTGGAGTTGGCGGAAAAGAATATGGCATTTGAAGTTATATATTACCCTAGGGCTAATTGGTGCAATTTTGTAGTAGATGCAAATGTTGTGGATGATGCTATGAAGATTGGTTGGGCATCTGGAATGAGAGTGAAGCTTCCTTTGAAGATTGATGAATCTTCAAATTCGAAGATGACATTTTTTCAGCCTCAAGGAACAATCTCTAATGTGTCCAGTGTCCCTAATTGGCGCATGCTTCAGGTGCTCTTCTTTTTTGGTCTTGTTGTAGTTTTATTTTATTTTTTGAATCAAGGGCTAATTATATTTGACTGCATAATGTTATAAATATTATTGTCGAAGAAGATTAGGCAATGATTCATTTAGACGTTATAGTCACACAATTCGCATTTCGCGTTGTTACGGGTTTGCGGTACACGACATGAGAAGAATTCGATGCATACGGAGGTATAAACCTTGCATCGATTCATAGTATTGTTACTACCTAATTAAGTATACTAAACTATCTTAATATTACAAAAAATTAGAAGGATTATATCGACAAGCTACAACAAATTAGCGCCCACTTCCGAAAACCTTTTTTTACAGCTCAACCAACACAATCCTTGCTGAAAACATTATTCAACGATTATGAGACATGATTACCAAAATACAGTTAGGAAAAATCACAACAGCTTCAATGCTCACAATCACAACAATAGCTAGCTGTGAAAACATTATTCAACGATTATGAGACATGATTACCAAAATACAGGAAAAATCACAACAGCTTCAATGCTCACAATAACAATAGCTAGCTGAATAATTATAGTAGTGTCATCTTTTTTCTCTCCACAATAGCAGCAGCAACAGTGGTATATATGTTTCAGCAAGAGGAAGAGTGAAGATATAGATAACTTGGAATGCAAACATAGAGTTACATTCCCTGAGAGCCTTGATCATTGTTGCTCCAAGTTTAAAGAGTCATGTTTTATCCCTTGATCAATTTAGTATACAAATATTAGGGTTAGGTGATAGGAGTGTAGGCTAGTATACTATCTATAGCTTTGTTGTGCCCTATAGGATAAGTTATATATAAAATTCTAATATTATTTGACCAACTAAGTTAGTCGTTAATTTACTGTTTTTTGGTGTTGTCATTATTTACATTCAACACTAATAAAAAGAAAATATTAGAACATCAATTTATATACAGCAGAGCTAATTTTAAGAGAATACTTTAGTTTGATTAGAAATACATTTGAAGTAATTTATAGTTGATACCAAAATCAATCCATAATTATGGATCAACTGCAACACAATTTGTTCTTGCACTTGTATCATTTGTGATCATTAGATGTGAAACCATGCACCCTTCTTGCTCCTCATATGATTGATTGATTGATGAAATGCGAGAATAAAAGAGTGACAAGTGTTTTCGGGCATATAAATAATGAAGAAGCTTCAGGTATCTTATGGAAAAGTATTAGTTATTTAGGTTACCATATTTTTATAATAAAATGCATTTGCTCTTATTCCTAAGGAGAATCTATATTTTACTCACATGTTAGTATTTGTTAAGAAACATTTGATGTGCATATACATACTATAACTTATCGTATATTTTAAATGACTGATATTGGATTCTTACCATTTTTATCTCTTTATCATAGGTTAACTGGGATGAACTTGAAATCTTGCAAAATCAAAACCGCATCAATCCCTGGCAAGTTGAACTTATTTCCCGCAATCCTGCAGTGCATCTACCATTTCTCTCAACAAAAAAGCCTAGACTAGTTCAGGATTCTACCTTGTTTAGTGATAACAAGGGTGATCCCTTCATTCCTATGATAGAGTTCCCTAAGAGATCTTTGAACCAAACATTGTTGAATTGCGGTTATTTTCCAGCAGGCATGCAGGGAGCCAGGCATGATCATTTATCTCTATCAGGTTTTTCCAACTCTTTGAATGATAATAGTTATGGTTTCTTTGTGAACAATCTAAACATTGACAGTCCCACATCTAACGACTTGTCGCCAAATAACCTTAGTAGTTTGAACATATTCGGGACAGATTTTGTTGAGACTCACAACAATAACACAAAAGGAGTTGGTTCTGGTTCAATTAAGTTATTTGGTAAGATCATTCAGACCGTTGAAAGTGATGTCCATGATTCTGGTTCCAAGGGAGAAGATTCCTCTAAGCGATCCAATGAAATTTGA
Protein sequence

>MS.gene010286.t1

MSSPPATMSVPPARRVKSKIWQTCAGPSVNVPKVRSKVYYFPQGHLEHACPSPNPQTITVVDGYRPSFPCIITAVDLLADPHTDEVFAKLLLSPVTEGQELPEVVDKEEDGDKVVSFVKTLTKSDSNNGGGFSVPRLCADLIFPKLDLNSPFPSQELSVTDVHDGVWKFAHVYRGRPKRHLFTTGWTPFVNTKKLVAGDSIVFMKNTAGDIVVGIRRNMKFTAAESKAVNNRKGDEGKENGLEVKGEGFSRGGRRGILTVKAVIDAVELAEKNMAFEVIYYPRANWCNFVVDANVVDDAMKIGWASGMRVKLPLKIDESSNSKMTFFQPQGTISNVSSVPNWRMLQVNWDELEILQNQNRINPWQVELISRNPAVHLPFLSTKKPRLVQDSTLFSDNKGDPFIPMIEFPKRSLNQTLLNCGYFPAGMQGARHDHLSLSGFSNSLNDNSYGFFVNNLNIDSPTSNDLSPNNLSSLNIFGTDFVETHNNNTKGVGSGSIKLFGKIIQTVESDVHDSGSKGEDSSKRSNEI