AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010294

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010294.t1 MTR_5g060950 96.089 358 14 0 16 373 1 358 0.0 719
MS.gene010294.t1 MTR_3g073560 65.517 348 114 3 16 361 1 344 2.32e-155 450
MS.gene010294.t1 MTR_3g087940 49.587 363 172 3 4 361 2 358 4.18e-117 344
MS.gene010294.t1 MTR_8g091650 47.126 348 177 4 14 361 13 353 4.94e-99 298
MS.gene010294.t1 MTR_8g091650 43.307 381 179 4 14 361 13 389 2.62e-94 287
MS.gene010294.t1 MTR_8g091650 52.347 277 131 1 85 361 5 280 5.90e-93 280
MS.gene010294.t1 MTR_8g040120 51.568 287 128 2 82 361 181 463 1.75e-89 277
MS.gene010294.t1 MTR_7g092750 46.667 300 153 2 69 361 60 359 3.28e-89 273
MS.gene010294.t1 MTR_7g089800 50.871 287 130 3 82 361 138 420 7.99e-89 274
MS.gene010294.t1 MTR_2g086420 51.429 280 129 2 82 361 194 466 8.14e-89 276
MS.gene010294.t1 MTR_2g086420 51.429 280 129 2 82 361 193 465 9.20e-89 276
MS.gene010294.t1 MTR_7g089800 50.871 287 130 3 82 361 167 449 1.90e-88 274
MS.gene010294.t1 MTR_7g092750 46.667 300 153 2 69 361 161 460 8.43e-88 273
MS.gene010294.t1 MTR_4g053250 45.981 311 155 5 52 361 160 458 3.50e-83 261
MS.gene010294.t1 MTR_5g054900 44.631 298 154 2 71 361 57 350 4.50e-79 247
MS.gene010294.t1 MTR_5g054900 44.631 298 154 2 71 361 138 431 3.65e-78 247
MS.gene010294.t1 MTR_5g054900 43.960 298 152 3 71 361 57 346 3.29e-75 237
MS.gene010294.t1 MTR_7g092750 42.667 300 139 3 69 361 161 434 7.51e-75 238
MS.gene010294.t1 MTR_5g054900 43.960 298 152 3 71 361 168 457 3.99e-74 237
MS.gene010294.t1 MTR_1g111310 46.367 289 149 3 77 360 416 703 2.31e-73 241
MS.gene010294.t1 MTR_1g111310 44.912 285 147 2 77 358 49 326 3.19e-66 223
MS.gene010294.t1 MTR_0126s0080 39.528 339 179 6 4 317 122 459 6.65e-65 214
MS.gene010294.t1 MTR_3g044820 43.195 169 89 3 77 238 40 208 6.06e-41 144
MS.gene010294.t1 MTR_1g090370 31.000 200 115 5 163 357 47 228 5.08e-15 73.9
MS.gene010294.t1 MTR_1g111320 64.000 50 18 0 82 131 146 195 1.60e-12 66.6
MS.gene010294.t1 MTR_7g068860 25.698 179 117 3 188 354 45 219 2.50e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010294.t1 AT1G77920 52.520 377 149 6 1 361 1 363 5.60e-122 357
MS.gene010294.t1 AT1G22070 50.269 372 160 4 14 365 17 383 6.37e-117 345
MS.gene010294.t1 AT5G10030 50.139 361 174 3 4 361 2 359 5.21e-115 339
MS.gene010294.t1 AT5G10030 50.139 361 174 3 4 361 2 359 5.21e-115 339
MS.gene010294.t1 AT5G10030 50.139 361 174 3 4 361 2 359 5.21e-115 339
MS.gene010294.t1 AT5G10030 50.139 361 174 3 4 361 2 359 5.21e-115 339
MS.gene010294.t1 AT5G10030 50.139 361 174 3 4 361 2 359 5.21e-115 339
MS.gene010294.t1 AT5G65210 48.649 370 183 4 4 369 2 368 2.15e-114 338
MS.gene010294.t1 AT5G65210 48.649 370 183 4 4 369 2 368 2.15e-114 338
MS.gene010294.t1 AT5G65210 48.649 370 183 4 4 369 2 368 2.15e-114 338
MS.gene010294.t1 AT5G65210 48.649 370 183 4 4 369 2 368 2.15e-114 338
MS.gene010294.t1 AT5G65210 48.649 370 183 4 4 369 2 368 2.15e-114 338
MS.gene010294.t1 AT5G65210 48.649 370 183 4 4 369 2 368 2.15e-114 338
MS.gene010294.t1 AT5G65210 53.512 299 135 2 72 369 3 298 9.09e-105 310
MS.gene010294.t1 AT1G08320 47.095 327 154 6 46 361 8 326 2.74e-91 278
MS.gene010294.t1 AT1G08320 47.095 327 154 6 46 361 8 326 2.74e-91 278
MS.gene010294.t1 AT1G08320 47.095 327 154 6 46 361 132 450 1.14e-89 278
MS.gene010294.t1 AT1G08320 47.095 327 154 6 46 361 132 450 1.14e-89 278
MS.gene010294.t1 AT1G08320 47.095 327 154 6 46 361 132 450 1.14e-89 278
MS.gene010294.t1 AT5G06839 47.541 305 145 2 72 361 108 412 8.10e-87 269
MS.gene010294.t1 AT5G06839 47.541 305 145 2 72 361 151 455 1.56e-86 270
MS.gene010294.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.14e-86 264
MS.gene010294.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.14e-86 264
MS.gene010294.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.14e-86 264
MS.gene010294.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.14e-86 264
MS.gene010294.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.14e-86 264
MS.gene010294.t1 AT5G06839 46.732 306 147 2 72 361 108 413 1.03e-85 266
MS.gene010294.t1 AT3G12250 48.601 286 137 2 82 361 19 300 2.50e-84 258
MS.gene010294.t1 AT3G12250 48.601 286 137 2 82 361 46 327 4.21e-84 259
MS.gene010294.t1 AT3G12250 48.601 286 137 2 82 361 46 327 4.21e-84 259
MS.gene010294.t1 AT3G12250 48.601 286 137 2 82 361 46 327 4.21e-84 259
MS.gene010294.t1 AT3G12250 48.601 286 137 2 82 361 46 327 4.21e-84 259
MS.gene010294.t1 AT3G12250 48.601 286 137 2 82 361 40 321 5.05e-84 258
MS.gene010294.t1 AT3G12250 48.601 286 137 2 82 361 71 352 2.11e-83 258
MS.gene010294.t1 AT5G06960 48.252 286 138 2 82 361 26 307 4.57e-83 256
MS.gene010294.t1 AT5G06960 48.252 286 138 2 82 361 46 327 4.96e-83 256
MS.gene010294.t1 AT5G06960 48.252 286 138 2 82 361 46 327 4.96e-83 256
MS.gene010294.t1 AT1G68640 44.631 298 153 3 72 361 156 449 2.34e-72 233
MS.gene010294.t1 AT1G58330 29.381 194 114 6 178 354 36 223 1.64e-12 67.0

Find 65 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTGGATGTCGGTTATA+TGG 0.184139 5.4:-47574312 MS.gene010294:CDS
TCTTTGTTTGAAATTGAATA+TGG 0.229069 5.4:-47574182 MS.gene010294:CDS
CAAGCTGATCCCTTGGATTT+CGG 0.267505 5.4:-47573164 MS.gene010294:CDS
TTTATACAGAAACAGTTTAT+TGG 0.277561 5.4:-47574327 MS.gene010294:CDS
TGCTCAAGGCTTGTTGGCTA+TGG 0.316587 5.4:-47572832 MS.gene010294:CDS
ATCCTCCTATCCAAAGAAAA+AGG 0.354680 5.4:+47573961 None:intergenic
TTATGTTCATCTTCAACATT+GGG 0.356119 5.4:+47575234 None:intergenic
TCATTCAAATTCTCAAGTTT+CGG 0.363992 5.4:+47573293 None:intergenic
GCTGAAGATGCACTGTCAAT+AGG 0.369583 5.4:-47573224 MS.gene010294:CDS
CTTATGTTCATCTTCAACAT+TGG 0.379233 5.4:+47575233 None:intergenic
GCTCAAGGCTTGTTGGCTAT+GGG 0.379556 5.4:-47572831 MS.gene010294:CDS
CTAACCTGAGTTTATTGTTC+CGG 0.383626 5.4:+47574283 None:intergenic
CTCAACTAATCGCGCCGAAA+AGG 0.387136 5.4:-47575343 MS.gene010294:CDS
TCAAGCTGCTCAAGGCTTGT+TGG 0.395796 5.4:-47572838 MS.gene010294:CDS
ACAGGCTTATGTTCAACAAC+TGG 0.406023 5.4:-47574512 MS.gene010294:intron
CTTTCAGATGGCTGCTGCCA+TGG 0.412683 5.4:-47573132 MS.gene010294:CDS
TATCGAGAAAACAAGAAAAC+AGG 0.417815 5.4:-47574452 MS.gene010294:intron
CTTTGCAGGCAGATCACCTT+AGG 0.420677 5.4:-47572902 MS.gene010294:intron
TTATTGGATGTCGGTTATAT+GGG 0.423086 5.4:-47574311 MS.gene010294:CDS
CCTCCCACATAGTCACTTGA+AGG 0.428980 5.4:+47575300 None:intergenic
AAGGTTCTCTTGATTCTTGA+GGG 0.435161 5.4:+47575085 None:intergenic
CCCTTGGATTTCGGAAACTA+CGG 0.436857 5.4:-47573155 MS.gene010294:CDS
CAAGAATCAAGAGAACCTTC+TGG 0.437311 5.4:-47575081 MS.gene010294:CDS
TACTGCTTCTCCTTTGTCCA+TGG 0.443791 5.4:+47573115 None:intergenic
CTGAAGATGCACTGTCAATA+GGG 0.444666 5.4:-47573223 MS.gene010294:CDS
GACAAAGGAGAAGCAGTAGA+AGG 0.456711 5.4:-47573110 MS.gene010294:CDS
AGTGTTGAGTAGGGTAGAAA+TGG 0.460652 5.4:+47572751 None:intergenic
CAACATTCAAGCTGATCCCT+TGG 0.462368 5.4:-47573171 MS.gene010294:CDS
TCTTAATTTGAGTGTTGAGT+AGG 0.465347 5.4:+47572741 None:intergenic
GTCGGTTATATGGGAACGTC+CGG 0.469360 5.4:-47574302 MS.gene010294:CDS
GAAGGTTCTCTTGATTCTTG+AGG 0.474792 5.4:+47575084 None:intergenic
TGTTGTTGATCATACTCTGC+TGG 0.478454 5.4:+47573189 None:intergenic
TCTATTTAATCTCTGGCGTA+TGG 0.481044 5.4:-47574001 MS.gene010294:CDS
ATGTCACGCATCTTATCAAT+TGG 0.485700 5.4:-47572861 MS.gene010294:CDS
CTCAAGGCTTGTTGGCTATG+GGG 0.490238 5.4:-47572830 MS.gene010294:CDS
CTTAATTTGAGTGTTGAGTA+GGG 0.496496 5.4:+47572742 None:intergenic
CATAAGTAGCATGGAGAACA+AGG 0.509417 5.4:-47575216 MS.gene010294:intron
CACATAGTCACTTGAAGGAA+TGG 0.522645 5.4:+47575305 None:intergenic
CATTCCTTCAAGTGACTATG+TGG 0.523116 5.4:-47575304 MS.gene010294:CDS
TCAAGGCTTGTTGGCTATGG+GGG 0.523504 5.4:-47572829 MS.gene010294:CDS
AGATGCACTGTCAATAGGGT+TGG 0.527361 5.4:-47573219 MS.gene010294:CDS
ATAACAGAGTTTGTTGCCTA+AGG 0.529419 5.4:+47572886 None:intergenic
TTGAAATTGAATATGGACGT+TGG 0.536052 5.4:-47574175 MS.gene010294:CDS
TCAATTGGTCAAGCTGCTCA+AGG 0.536456 5.4:-47572846 MS.gene010294:CDS
TTCGCACTCTTAGTTCATTG+TGG 0.540430 5.4:-47572791 MS.gene010294:CDS
TATTCAAGAACCTGTTCCTA+AGG 0.542554 5.4:-47575043 MS.gene010294:intron
TAGATTGAAGCTCATGCAGT+TGG 0.542577 5.4:-47574482 MS.gene010294:CDS
CGGAAACTACGGCTTTCAGA+TGG 0.543160 5.4:-47573144 MS.gene010294:CDS
AATGAAAGCAGAAGCTGCAA+AGG 0.554661 5.4:-47574033 MS.gene010294:CDS
AGAAACAGTTTATTGGATGT+CGG 0.555658 5.4:-47574320 MS.gene010294:CDS
GCCGTAGTTTCCGAAATCCA+AGG 0.586415 5.4:+47573154 None:intergenic
GAAGATGAACATAAGTAGCA+TGG 0.592197 5.4:-47575225 MS.gene010294:CDS
TGTACATTTAGAATTTGTGA+TGG 0.598170 5.4:+47573933 None:intergenic
GCGCGGCTGCTCGAAAATGT+CGG 0.599361 5.4:-47574616 MS.gene010294:CDS
ATGGCTGCTGCCATGGACAA+AGG 0.602885 5.4:-47573125 MS.gene010294:CDS
GTCTCTTGATCATCTCCAGA+AGG 0.603267 5.4:+47575066 None:intergenic
CTCTGGCGTATGGAAATCAC+CGG 0.616184 5.4:-47573991 MS.gene010294:CDS
TTCATCTACTTGTCGAGAGT+AGG 0.630773 5.4:-47574082 MS.gene010294:CDS
CCGTAGTTTCCGAAATCCAA+GGG 0.636532 5.4:+47573155 None:intergenic
CCTTCAAGTGACTATGTGGG+AGG 0.650193 5.4:-47575300 MS.gene010294:CDS
ATTCCTTCAAGTGACTATGT+GGG 0.664208 5.4:-47575303 MS.gene010294:CDS
ACGTCCGGAACAATAAACTC+AGG 0.666698 5.4:-47574287 MS.gene010294:intron
CTTGCTGAGATGATAAACGC+AGG 0.683767 5.4:+47573246 None:intergenic
TATGTTCATCTTCAACATTG+GGG 0.698417 5.4:+47575235 None:intergenic
ACGTCAAGCACAGAATCGCG+CGG 0.719929 5.4:-47574633 MS.gene010294:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAATATAAAATCATAATT+TGG - chr5.4:47575037-47575056 MS.gene010294:intron 10.0%
!! AATAAGATAATAGTAATTAG+TGG + chr5.4:47574202-47574221 None:intergenic 15.0%
!! AGTAGAAAAAATTGAAATAA+CGG - chr5.4:47573106-47573125 MS.gene010294:CDS 15.0%
!! ATAAGATAATAGTAATTAGT+GGG + chr5.4:47574201-47574220 None:intergenic 15.0%
!! GTTGTAAAAATTGAATTTAT+AGG - chr5.4:47573527-47573546 MS.gene010294:intron 15.0%
!! TAAAAATTGAATTTATAGGA+AGG - chr5.4:47573531-47573550 MS.gene010294:intron 15.0%
!!! AATTAATTTTTTTTGACAGA+AGG + chr5.4:47573375-47573394 None:intergenic 15.0%
!! TATAGTCTATATTCGTTAAT+AGG - chr5.4:47574223-47574242 MS.gene010294:intron 20.0%
!! TTTATACAGAAACAGTTTAT+TGG - chr5.4:47573781-47573800 MS.gene010294:intron 20.0%
!! TTTATATAAAATAGAGGAAG+AGG + chr5.4:47573711-47573730 None:intergenic 20.0%
!!! ATAGTTTTAACATAGTATCA+TGG - chr5.4:47573335-47573354 MS.gene010294:intron 20.0%
!!! GAATTTCTATTTTGTTTCTA+TGG - chr5.4:47572930-47572949 MS.gene010294:intron 20.0%
!!! TCTTTGTTTGAAATTGAATA+TGG - chr5.4:47573926-47573945 MS.gene010294:intron 20.0%
! ATCTGTAAGAAAAAGTCAAA+TGG + chr5.4:47574788-47574807 None:intergenic 25.0%
! GTTTCTCAATATCTATGTAT+AGG - chr5.4:47573445-47573464 MS.gene010294:intron 25.0%
! TCATTCAAATTCTCAAGTTT+CGG + chr5.4:47574818-47574837 None:intergenic 25.0%
! TCGTTAATAGGTCAAAATAA+AGG - chr5.4:47574235-47574254 MS.gene010294:intron 25.0%
! TTAAAGAGAATTGTTTAGCT+TGG - chr5.4:47573393-47573412 MS.gene010294:intron 25.0%
! TTATGTTCATCTTCAACATT+GGG + chr5.4:47572877-47572896 None:intergenic 25.0%
! TTTGAAAAAGATGTGAGTAT+TGG + chr5.4:47574057-47574076 None:intergenic 25.0%
!! CAAAACATAGAACAAGTTTT+TGG + chr5.4:47574666-47574685 None:intergenic 25.0%
!! GATGTTTTCTATTTAATCTC+TGG - chr5.4:47574100-47574119 MS.gene010294:CDS 25.0%
!! TGATGTAATTTTATCACCTT+AGG + chr5.4:47573084-47573103 None:intergenic 25.0%
!! TGTACATTTAGAATTTGTGA+TGG + chr5.4:47574178-47574197 None:intergenic 25.0%
!!! AAGGATGACGATTTTTTATA+TGG + chr5.4:47573687-47573706 None:intergenic 25.0%
AAAGACGAGAAAAATAACAC+TGG - chr5.4:47573137-47573156 MS.gene010294:CDS 30.0%
AATTTAAACTTCGTCTCTTG+CGG - chr5.4:47574374-47574393 MS.gene010294:intron 30.0%
ACGAATTTACAACTGTTTAG+AGG - chr5.4:47574350-47574369 MS.gene010294:intron 30.0%
AGAAACAGTTTATTGGATGT+CGG - chr5.4:47573788-47573807 MS.gene010294:intron 30.0%
ATAAAATAGAGGAAGAGGTA+AGG + chr5.4:47573706-47573725 None:intergenic 30.0%
CTTATGTTCATCTTCAACAT+TGG + chr5.4:47572878-47572897 None:intergenic 30.0%
GTGTATTAGAATTCAAACGT+GGG - chr5.4:47575115-47575134 MS.gene010294:intron 30.0%
TATCCATATTTATCCTTGCA+GGG - chr5.4:47573758-47573777 MS.gene010294:intron 30.0%
TATCGAGAAAACAAGAAAAC+AGG - chr5.4:47573656-47573675 MS.gene010294:intron 30.0%
TATGTTCATCTTCAACATTG+GGG + chr5.4:47572876-47572895 None:intergenic 30.0%
TGTGTATTAGAATTCAAACG+TGG - chr5.4:47575114-47575133 MS.gene010294:intron 30.0%
TTATCCATATTTATCCTTGC+AGG - chr5.4:47573757-47573776 MS.gene010294:intron 30.0%
TTGAAATTGAATATGGACGT+TGG - chr5.4:47573933-47573952 MS.gene010294:intron 30.0%
TTTGTATGTTTGTGATTCAC+AGG - chr5.4:47573578-47573597 MS.gene010294:intron 30.0%
! AATTTTATCACCTTAGGAAC+AGG + chr5.4:47573078-47573097 None:intergenic 30.0%
! TCATACATGTTCATCCTTTT+CGG + chr5.4:47572782-47572801 None:intergenic 30.0%
! TTATTGGATGTCGGTTATAT+GGG - chr5.4:47573797-47573816 MS.gene010294:intron 30.0%
! TTTATTGGATGTCGGTTATA+TGG - chr5.4:47573796-47573815 MS.gene010294:intron 30.0%
!! AGTAGAAGGTTTTGTAATTC+AGG - chr5.4:47575012-47575031 MS.gene010294:intron 30.0%
AAACCCTGCAAGGATAAATA+TGG + chr5.4:47573764-47573783 None:intergenic 35.0%
AAGGTTCTCTTGATTCTTGA+GGG + chr5.4:47573026-47573045 None:intergenic 35.0%
ATCCTCCTATCCAAAGAAAA+AGG + chr5.4:47574150-47574169 None:intergenic 35.0%
ATGTCACGCATCTTATCAAT+TGG - chr5.4:47575247-47575266 MS.gene010294:CDS 35.0%
ATTCCTTCAAGTGACTATGT+GGG - chr5.4:47572805-47572824 MS.gene010294:CDS 35.0%
CTAACCTGAGTTTATTGTTC+CGG + chr5.4:47573828-47573847 None:intergenic 35.0%
GAAGATGAACATAAGTAGCA+TGG - chr5.4:47572883-47572902 MS.gene010294:CDS 35.0%
TAGCTTGGAAGAGTTCATAA+TGG - chr5.4:47573408-47573427 MS.gene010294:intron 35.0%
TATTAGAATTCAAACGTGGG+TGG - chr5.4:47575118-47575137 MS.gene010294:intron 35.0%
TATTCAAGAACCTGTTCCTA+AGG - chr5.4:47573065-47573084 MS.gene010294:intron 35.0%
TCAGCTCAGTGGTAAAATAA+TGG + chr5.4:47574408-47574427 None:intergenic 35.0%
TCTATTTAATCTCTGGCGTA+TGG - chr5.4:47574107-47574126 MS.gene010294:CDS 35.0%
! GCATTTTTCTTCTTCTTTGC+AGG - chr5.4:47575192-47575211 MS.gene010294:intron 35.0%
! TGTCATTTCATGTAAACCTC+AGG - chr5.4:47574703-47574722 MS.gene010294:intron 35.0%
!! ATAACAGAGTTTGTTGCCTA+AGG + chr5.4:47575225-47575244 None:intergenic 35.0%
!! TGACTTTTTCTTACAGATCG+TGG - chr5.4:47574790-47574809 MS.gene010294:intron 35.0%
AATGAAAGCAGAAGCTGCAA+AGG - chr5.4:47574075-47574094 MS.gene010294:CDS 40.0%
ACAGGCTTATGTTCAACAAC+TGG - chr5.4:47573596-47573615 MS.gene010294:intron 40.0%
ATCACACATAACATGTGTCG+TGG - chr5.4:47575082-47575101 MS.gene010294:CDS 40.0%
CAAGAATCAAGAGAACCTTC+TGG - chr5.4:47573027-47573046 MS.gene010294:intron 40.0%
CACATAGTCACTTGAAGGAA+TGG + chr5.4:47572806-47572825 None:intergenic 40.0%
CATAAGTAGCATGGAGAACA+AGG - chr5.4:47572892-47572911 MS.gene010294:CDS 40.0%
CATTCCTTCAAGTGACTATG+TGG - chr5.4:47572804-47572823 MS.gene010294:CDS 40.0%
CTGAAGATGCACTGTCAATA+GGG - chr5.4:47574885-47574904 MS.gene010294:intron 40.0%
GAAGGTTCTCTTGATTCTTG+AGG + chr5.4:47573027-47573046 None:intergenic 40.0%
GTTTCTGTATAAACCCTGCA+AGG + chr5.4:47573774-47573793 None:intergenic 40.0%
TAGATTGAAGCTCATGCAGT+TGG - chr5.4:47573626-47573645 MS.gene010294:intron 40.0%
TGTTGTTGATCATACTCTGC+TGG + chr5.4:47574922-47574941 None:intergenic 40.0%
TTCATCTACTTGTCGAGAGT+AGG - chr5.4:47574026-47574045 MS.gene010294:CDS 40.0%
TTCGCACTCTTAGTTCATTG+TGG - chr5.4:47575317-47575336 MS.gene010294:CDS 40.0%
! AAAATGTCGGCTGCGAAAAA+AGG - chr5.4:47573505-47573524 MS.gene010294:intron 40.0%
! AGGACATGTTTCTATAGCGT+CGG - chr5.4:47574723-47574742 MS.gene010294:intron 40.0%
! AGTGTTGAGTAGGGTAGAAA+TGG + chr5.4:47575360-47575379 None:intergenic 40.0%
! CTTATGATTTGTCAGCTCAG+TGG + chr5.4:47574419-47574438 None:intergenic 40.0%
! GAACGCCTTTTTCTTTGGAT+AGG - chr5.4:47574142-47574161 MS.gene010294:CDS 40.0%
ACGTCCGGAACAATAAACTC+AGG - chr5.4:47573821-47573840 MS.gene010294:intron 45.0%
AGATGCACTGTCAATAGGGT+TGG - chr5.4:47574889-47574908 MS.gene010294:intron 45.0%
CAACATTCAAGCTGATCCCT+TGG - chr5.4:47574937-47574956 MS.gene010294:intron 45.0%
CAAGCTGATCCCTTGGATTT+CGG - chr5.4:47574944-47574963 MS.gene010294:intron 45.0%
CCCTTGGATTTCGGAAACTA+CGG - chr5.4:47574953-47574972 MS.gene010294:intron 45.0%
CCGTAGTTTCCGAAATCCAA+GGG + chr5.4:47574956-47574975 None:intergenic 45.0%
CGCTATAGAAACATGTCCTG+AGG + chr5.4:47574722-47574741 None:intergenic 45.0%
CTTCGTCTCTTGCGGTTATA+TGG - chr5.4:47574382-47574401 MS.gene010294:intron 45.0%
CTTGCTGAGATGATAAACGC+AGG + chr5.4:47574865-47574884 None:intergenic 45.0%
GACAAAGGAGAAGCAGTAGA+AGG - chr5.4:47574998-47575017 MS.gene010294:intron 45.0%
GCTGAAGATGCACTGTCAAT+AGG - chr5.4:47574884-47574903 MS.gene010294:intron 45.0%
GTCTCTTGATCATCTCCAGA+AGG + chr5.4:47573045-47573064 None:intergenic 45.0%
TACTGCTTCTCCTTTGTCCA+TGG + chr5.4:47574996-47575015 None:intergenic 45.0%
TCAATTGGTCAAGCTGCTCA+AGG - chr5.4:47575262-47575281 MS.gene010294:CDS 45.0%
TCCTGTCATGTTTCGAAGCA+GGG - chr5.4:47573900-47573919 MS.gene010294:intron 45.0%
! CGCCTTTTTCTTTGGATAGG+AGG - chr5.4:47574145-47574164 MS.gene010294:CDS 45.0%
!! CAAAGAAAAAGGCGTTCGAC+CGG + chr5.4:47574139-47574158 None:intergenic 45.0%
!! AAATAATTTATATAAAATAG+AGG + chr5.4:47573717-47573736 None:intergenic 5.0%
!!! TTTTAAAAAATATAATTTTG+AGG + chr5.4:47573877-47573896 None:intergenic 5.0%
CCTCCCACATAGTCACTTGA+AGG + chr5.4:47572811-47572830 None:intergenic 50.0%
CCTTCAAGTGACTATGTGGG+AGG - chr5.4:47572808-47572827 MS.gene010294:CDS 50.0%
CGGAAACTACGGCTTTCAGA+TGG - chr5.4:47574964-47574983 MS.gene010294:intron 50.0%
CTCAACTAATCGCGCCGAAA+AGG - chr5.4:47572765-47572784 MS.gene010294:CDS 50.0%
CTCTGGCGTATGGAAATCAC+CGG - chr5.4:47574117-47574136 MS.gene010294:CDS 50.0%
CTTTGCAGGCAGATCACCTT+AGG - chr5.4:47575206-47575225 MS.gene010294:intron 50.0%
GCCGTAGTTTCCGAAATCCA+AGG + chr5.4:47574957-47574976 None:intergenic 50.0%
GTCCTGTCATGTTTCGAAGC+AGG - chr5.4:47573899-47573918 MS.gene010294:intron 50.0%
GTCGGTTATATGGGAACGTC+CGG - chr5.4:47573806-47573825 MS.gene010294:intron 50.0%
TCAAGCTGCTCAAGGCTTGT+TGG - chr5.4:47575270-47575289 MS.gene010294:CDS 50.0%
TCCCTGCTTCGAAACATGAC+AGG + chr5.4:47573904-47573923 None:intergenic 50.0%
! CGGTCGAACGCCTTTTTCTT+TGG - chr5.4:47574137-47574156 MS.gene010294:CDS 50.0%
!! AAGCGTGTGTTGACTAGACG+AGG - chr5.4:47574436-47574455 MS.gene010294:intron 50.0%
!! CTCAAGGCTTGTTGGCTATG+GGG - chr5.4:47575278-47575297 MS.gene010294:CDS 50.0%
!! GCTCAAGGCTTGTTGGCTAT+GGG - chr5.4:47575277-47575296 MS.gene010294:CDS 50.0%
!! TCAAGGCTTGTTGGCTATGG+GGG - chr5.4:47575279-47575298 MS.gene010294:CDS 50.0%
!! TGCTCAAGGCTTGTTGGCTA+TGG - chr5.4:47575276-47575295 MS.gene010294:CDS 50.0%
ACGTCAAGCACAGAATCGCG+CGG - chr5.4:47573475-47573494 MS.gene010294:intron 55.0%
ATGGCTGCTGCCATGGACAA+AGG - chr5.4:47574983-47575002 MS.gene010294:intron 55.0%
CTTTCAGATGGCTGCTGCCA+TGG - chr5.4:47574976-47574995 MS.gene010294:intron 55.0%
GCGCGGCTGCTCGAAAATGT+CGG - chr5.4:47573492-47573511 MS.gene010294:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 47572743 47575387 47572743 ID=MS.gene010294
chr5.4 mRNA 47572743 47575387 47572743 ID=MS.gene010294.t1;Parent=MS.gene010294
chr5.4 exon 47575217 47575387 47575217 ID=MS.gene010294.t1.exon1;Parent=MS.gene010294.t1
chr5.4 CDS 47575217 47575387 47575217 ID=cds.MS.gene010294.t1;Parent=MS.gene010294.t1
chr5.4 exon 47575044 47575118 47575044 ID=MS.gene010294.t1.exon2;Parent=MS.gene010294.t1
chr5.4 CDS 47575044 47575118 47575044 ID=cds.MS.gene010294.t1;Parent=MS.gene010294.t1
chr5.4 exon 47574604 47574663 47574604 ID=MS.gene010294.t1.exon3;Parent=MS.gene010294.t1
chr5.4 CDS 47574604 47574663 47574604 ID=cds.MS.gene010294.t1;Parent=MS.gene010294.t1
chr5.4 exon 47574453 47574530 47574453 ID=MS.gene010294.t1.exon4;Parent=MS.gene010294.t1
chr5.4 CDS 47574453 47574530 47574453 ID=cds.MS.gene010294.t1;Parent=MS.gene010294.t1
chr5.4 exon 47574288 47574351 47574288 ID=MS.gene010294.t1.exon5;Parent=MS.gene010294.t1
chr5.4 CDS 47574288 47574351 47574288 ID=cds.MS.gene010294.t1;Parent=MS.gene010294.t1
chr5.4 exon 47573938 47574209 47573938 ID=MS.gene010294.t1.exon6;Parent=MS.gene010294.t1
chr5.4 CDS 47573938 47574209 47573938 ID=cds.MS.gene010294.t1;Parent=MS.gene010294.t1
chr5.4 exon 47573097 47573324 47573097 ID=MS.gene010294.t1.exon7;Parent=MS.gene010294.t1
chr5.4 CDS 47573097 47573324 47573097 ID=cds.MS.gene010294.t1;Parent=MS.gene010294.t1
chr5.4 exon 47572743 47572916 47572743 ID=MS.gene010294.t1.exon8;Parent=MS.gene010294.t1
chr5.4 CDS 47572743 47572916 47572743 ID=cds.MS.gene010294.t1;Parent=MS.gene010294.t1
Gene Sequence

>MS.gene010294

ATGAACTCTTCTTCATCATCAACTCAACTAATCGCGCCGAAAAGGATGAACATGTATGAGCCATTCCTTCAAGTGACTATGTGGGAGGATAGCTTTAAAGTTGATCATAGTGCTTTGAATTCAATTGCTTCCCCAATGTTGAAGATGAACATAAGTAGCATGGAGAACAAGGTGAAATTGAACTCATGAATTTCTATTTTGTTTCTATGGTTATCTATGTTTTATGAGAAAACTAGTTGACAATAGTCTTTTGAATTTTATTTGTATAGTCTGAATGTATCCCTCAAGAATCAAGAGAACCTTCTGGAGATGATCAAGAGACTATTCAAGAACCTGTTCCTAAGGTGATAAAATTACATCACTAGTAGAAAAAATTGAAATAACGGCAGAAATTAAAGACGAGAAAAATAACACTGGTTACTAAATTTAGCGTTTCTAACACTTAGAGACGCTTCTTCAAAAAAAAAAAACACTTAGAGACGCATTTTGTCTTTTTTTGTCTCTAAATTTTTGTCATTATTTCAACTTTTTTAAGTAGTGATTTATATTTCTTTTATATATTTAGTCATGATAATTTTCATGTCTCATTTTAATAGTTTTAACATAGTATCATGGAGTTTGAGAAGTGACCTTCTGTCAAAAAAAATTAATTAAAGAGAATTGTTTAGCTTGGAAGAGTTCATAATGGATTTTGACATTATAGTTTCTCAATATCTATGTATAGGTACTACGACGTCAAGCACAGAATCGCGCGGCTGCTCGAAAATGTCGGCTGCGAAAAAAGGTTGTAAAAATTGAATTTATAGGAAGGAAGCTACTAGTGTTTATGATCTTATTTGTATGTTTGTGATTCACAGGCTTATGTTCAACAACTGGAAACGAGTAGATTGAAGCTCATGCAGTTGGAATTAGATATCGAGAAAACAAGAAAACAGGTGATACCATATAAAAAATCGTCATCCTTACCTCTTCCTCTATTTTATATAAATTATTTCATCTATCATTCATAGAGATTTATCCATATTTATCCTTGCAGGGTTTATACAGAAACAGTTTATTGGATGTCGGTTATATGGGAACGTCCGGAACAATAAACTCAGGTTAGTGATTGATAATTGTAGTTTTAAGTTTCCTCAAAATTATATTTTTTAAAATAGTCCTGTCATGTTTCGAAGCAGGGATATCTTTGTTTGAAATTGAATATGGACGTTGGATTGAAGAACAAGATCAACAAAACGAAGAACTAAGAAATGCATTGCAAACTAATGCATCTGATATACATCTTCATCTACTTGTCGAGAGTAGGTTGAACCAATACTCACATCTTTTTCAAATGAAAGCAGAAGCTGCAAAGGCTGATGTTTTCTATTTAATCTCTGGCGTATGGAAATCACCGGTCGAACGCCTTTTTCTTTGGATAGGAGGATCTCGTCCATCACAAATTCTAAATGTACACCCACTAATTACTATTATCTTATTTTATAGTCTATATTCGTTAATAGGTCAAAATAAAGGAGCTTTATATATAATCTTATTTGTTGAAATTTTGTTAATATATTCTTGATCAGTAGTTTATAGAAACTAGAATCTGCTTATAATTACTATTGACGAATTTACAACTGTTTAGAGGAAATTTAAACTTCGTCTCTTGCGGTTATATGGCCATTATTTTACCACTGAGCTGACAAATCATAAGCGTGTGTTGACTAGACGAGGAGTACTAATTATTTGTTGAAATTTTGTTAATATATTCTTGATCAGTAGTTTATAGAAACTAGAATCTGCTTATAATTACTATTGACGAATTTACAACTGTTTAGAGGAAATTTAAACTTCGTCTCTTGCGGTTATATGGCCATTATTTTACCACTGAGCTGACAAATCATAAGCGTGTGTTGACTAGACGAGGAGTACTAATTGCCAAAAACTTGTTCTATGTTTTGTAAATTGTTAAACATAATGTCATTTCATGTAAACCTCAGGACATGTTTCTATAGCGTCGGTTGATACATGTAGCTAATCTATCAATGATTTTGTACAAACCATTTGACTTTTTCTTACAGATCGTGGTACCGAAACTTGAGAATTTGAATGATCAACAAATTGCTAGCATTAACAACCTGCGTTTATCATCTCAGCAAGCTGAAGATGCACTGTCAATAGGGTTGGAGAAACTCCAGCAGAGTATGATCAACAACATTCAAGCTGATCCCTTGGATTTCGGAAACTACGGCTTTCAGATGGCTGCTGCCATGGACAAAGGAGAAGCAGTAGAAGGTTTTGTAATTCAGGTAATCAATATAAAATCATAATTTGGTCTTGTTTGTTGTTTGTTATCAATCACACATAACATGTGTCGTGGAAACATTAATGTGTATTAGAATTCAAACGTGGGTGGTTAGTCTTGCATCGATTTTTCTTTTTTCGCACTTTCTACAGCTAAATTGAAGCATTTTTCTTCTTCTTTGCAGGCAGATCACCTTAGGCAACAAACTCTGTTATACATGTCACGCATCTTATCAATTGGTCAAGCTGCTCAAGGCTTGTTGGCTATGGGGGAATACTTTCATCGTCTTCGCACTCTTAGTTCATTGTGGACTGCTCGTTCATGTCACCATTTCTACCCTACTCAACACTCAAATTAA
Protein sequence

>MS.gene010294.t1

MNSSSSSTQLIAPKRMNMYEPFLQVTMWEDSFKVDHSALNSIASPMLKMNISSMENKSECIPQESREPSGDDQETIQEPVPKVLRRQAQNRAAARKCRLRKKAYVQQLETSRLKLMQLELDIEKTRKQGLYRNSLLDVGYMGTSGTINSGISLFEIEYGRWIEEQDQQNEELRNALQTNASDIHLHLLVESRLNQYSHLFQMKAEAAKADVFYLISGVWKSPVERLFLWIGGSRPSQILNIVVPKLENLNDQQIASINNLRLSSQQAEDALSIGLEKLQQSMINNIQADPLDFGNYGFQMAAAMDKGEAVEGFVIQADHLRQQTLLYMSRILSIGQAAQGLLAMGEYFHRLRTLSSLWTARSCHHFYPTQHSN