AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010306

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010306.t1 MTR_5g060780 66.935 372 102 8 1 357 9 374 2.06e-161 465
MS.gene010306.t1 MTR_5g460920 66.667 372 103 8 1 357 9 374 2.17e-160 462
MS.gene010306.t1 MTR_5g061890 67.493 363 98 7 1 348 9 366 2.22e-160 462
MS.gene010306.t1 MTR_5g060630 53.301 409 125 10 1 345 9 415 5.57e-126 379
MS.gene010306.t1 MTR_5g460740 53.301 409 125 10 1 345 9 415 5.57e-126 379
MS.gene010306.t1 MTR_5g061220 49.195 435 170 10 1 388 16 446 2.82e-125 377
MS.gene010306.t1 MTR_1g058210 53.333 360 143 9 1 341 9 362 1.36e-116 353
MS.gene010306.t1 MTR_2g024430 49.459 370 158 11 1 351 17 376 8.76e-102 317
MS.gene010306.t1 MTR_5g040880 51.205 332 138 9 1 316 8 331 7.07e-95 299
MS.gene010306.t1 MTR_5g082140 40.876 411 205 13 1 385 9 407 1.29e-85 273
MS.gene010306.t1 MTR_2g006270 45.225 356 169 9 4 341 13 360 3.20e-84 269
MS.gene010306.t1 MTR_2g006380 43.553 349 168 10 4 333 20 358 2.03e-80 259
MS.gene010306.t1 MTR_5g040740 49.684 316 131 9 1 297 9 315 3.40e-80 257
MS.gene010306.t1 MTR_5g060770 50.485 309 94 8 53 354 7 263 7.18e-74 239
MS.gene010306.t1 MTR_3g073420 35.586 503 239 17 1 433 16 503 5.83e-65 222
MS.gene010306.t1 MTR_5g062970 54.327 208 73 7 3 193 37 239 6.24e-64 209
MS.gene010306.t1 MTR_1g064430 33.333 453 211 14 1 373 24 465 4.43e-58 205
MS.gene010306.t1 MTR_1g064430 33.333 453 211 14 1 373 37 478 4.96e-58 206
MS.gene010306.t1 MTR_5g460910 42.945 326 98 10 40 354 10 258 7.28e-55 188
MS.gene010306.t1 MTR_2g094570 34.375 384 180 12 1 322 8 381 7.60e-54 194
MS.gene010306.t1 MTR_2g094570 34.375 384 180 12 1 322 8 381 1.20e-53 193
MS.gene010306.t1 MTR_2g094570 34.375 384 180 12 1 322 64 437 1.54e-53 194
MS.gene010306.t1 MTR_1g094960 33.738 412 188 15 1 347 16 407 5.05e-51 185
MS.gene010306.t1 MTR_4g058930 34.805 385 173 12 1 317 9 383 2.49e-49 181
MS.gene010306.t1 MTR_4g058930 34.805 385 173 12 1 317 9 383 3.04e-49 181
MS.gene010306.t1 MTR_7g101280 29.565 460 222 14 1 373 28 472 2.93e-47 175
MS.gene010306.t1 MTR_0041s0110 29.565 460 222 14 1 373 28 472 2.93e-47 175
MS.gene010306.t1 MTR_2g005240 32.518 409 206 15 4 377 23 396 1.20e-42 162
MS.gene010306.t1 MTR_2g018690 33.795 361 185 16 1 320 20 367 7.88e-39 152
MS.gene010306.t1 MTR_8g100050 31.548 336 204 9 4 316 59 391 3.07e-38 150
MS.gene010306.t1 MTR_7g101275 31.875 320 171 10 14 301 2 306 3.56e-38 145
MS.gene010306.t1 MTR_8g100050 31.548 336 204 9 4 316 59 391 4.25e-38 150
MS.gene010306.t1 MTR_8g079492 33.058 363 184 16 1 320 20 366 8.43e-38 149
MS.gene010306.t1 MTR_8g079492 33.058 363 184 16 1 320 20 366 9.67e-38 149
MS.gene010306.t1 MTR_1g024025 32.123 358 202 12 1 324 40 390 2.03e-37 148
MS.gene010306.t1 MTR_5g076270 32.782 363 186 16 1 320 21 368 2.34e-36 144
MS.gene010306.t1 MTR_2g043250 31.977 344 198 12 1 316 20 355 2.41e-36 145
MS.gene010306.t1 MTR_4g021580 30.458 371 213 15 2 343 17 371 3.50e-36 143
MS.gene010306.t1 MTR_4g021580 30.458 371 213 15 2 343 17 371 5.26e-36 142
MS.gene010306.t1 MTR_8g027440 31.897 348 212 12 4 331 29 371 2.36e-35 141
MS.gene010306.t1 MTR_7g062540 28.726 369 211 13 4 336 14 366 3.48e-35 140
MS.gene010306.t1 MTR_3g064050 31.768 362 191 15 1 320 23 370 4.61e-35 140
MS.gene010306.t1 MTR_2g014770 30.337 356 202 14 4 325 53 396 4.65e-35 139
MS.gene010306.t1 MTR_2g014770 30.337 356 202 14 4 325 53 396 6.27e-35 139
MS.gene010306.t1 MTR_2g093740 32.153 367 207 13 4 335 56 415 6.76e-35 139
MS.gene010306.t1 MTR_2g093740 32.153 367 207 13 4 335 56 415 8.10e-35 139
MS.gene010306.t1 MTR_2g093740 32.153 367 207 13 4 335 56 415 1.01e-34 139
MS.gene010306.t1 MTR_4g088210 31.105 389 216 15 4 354 38 412 1.29e-34 139
MS.gene010306.t1 MTR_4g088210 31.105 389 216 15 4 354 38 412 1.73e-34 138
MS.gene010306.t1 MTR_4g124900 31.594 345 198 14 1 316 20 355 4.19e-34 138
MS.gene010306.t1 MTR_4g124900 31.594 345 198 14 1 316 20 355 4.29e-34 138
MS.gene010306.t1 MTR_5g062960 43.716 183 86 5 211 390 1 169 4.69e-34 128
MS.gene010306.t1 MTR_2g093740 31.053 380 207 13 4 335 56 428 5.13e-33 134
MS.gene010306.t1 MTR_2g093740 31.053 380 207 13 4 335 56 428 5.63e-33 134
MS.gene010306.t1 MTR_2g093740 31.053 380 207 13 4 335 56 428 7.09e-33 134
MS.gene010306.t1 MTR_0041s0120 30.631 333 170 12 14 301 2 318 1.87e-31 126
MS.gene010306.t1 MTR_8g101360 30.028 353 195 10 1 316 28 365 1.17e-29 124
MS.gene010306.t1 MTR_4g060460 30.376 372 213 12 4 343 54 411 5.52e-29 122
MS.gene010306.t1 MTR_4g060460 30.376 372 213 12 4 343 54 411 6.00e-29 122
MS.gene010306.t1 MTR_8g079492 31.419 296 154 13 62 320 1 284 3.59e-24 107
MS.gene010306.t1 MTR_2g093740 30.970 268 158 9 93 335 20 285 2.76e-20 95.1
MS.gene010306.t1 MTR_2g093740 30.970 268 158 9 93 335 20 285 2.88e-20 95.1
MS.gene010306.t1 MTR_2g093740 29.537 281 158 9 93 335 20 298 9.86e-19 90.1
MS.gene010306.t1 MTR_2g093740 29.537 281 158 9 93 335 20 298 1.16e-18 90.1
MS.gene010306.t1 MTR_8g446900 27.397 292 169 12 53 316 12 288 1.39e-18 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010306.t1 AT1G77850 34.163 442 211 15 1 373 16 446 1.97e-66 226
MS.gene010306.t1 AT1G77850 34.163 442 211 15 1 373 16 446 2.15e-66 226
MS.gene010306.t1 AT4G30080 32.613 463 220 15 1 386 16 463 6.94e-53 191
MS.gene010306.t1 AT3G61830 34.447 389 206 17 3 362 24 392 3.83e-46 171
MS.gene010306.t1 AT2G28350 33.165 395 167 15 1 317 7 382 6.51e-46 172
MS.gene010306.t1 AT2G28350 33.165 395 167 15 1 317 7 382 7.74e-46 171
MS.gene010306.t1 AT2G46530 33.784 370 197 16 3 343 20 370 7.90e-44 164
MS.gene010306.t1 AT2G46530 33.784 370 197 16 3 343 41 391 1.18e-43 164
MS.gene010306.t1 AT5G37020 32.507 363 187 17 1 320 19 366 1.50e-39 154
MS.gene010306.t1 AT1G59750 32.915 398 222 15 4 377 22 398 2.07e-39 152
MS.gene010306.t1 AT5G37020 32.507 363 187 17 1 320 19 366 2.15e-39 154
MS.gene010306.t1 AT1G59750 33.531 337 195 11 4 316 22 353 2.47e-39 152
MS.gene010306.t1 AT1G59750 33.531 337 195 11 4 316 22 353 2.47e-39 152
MS.gene010306.t1 AT1G59750 33.531 337 195 11 4 316 22 353 2.47e-39 152
MS.gene010306.t1 AT1G30330 32.500 360 191 14 1 320 20 367 2.99e-38 150
MS.gene010306.t1 AT1G30330 32.500 360 191 14 1 320 22 369 3.02e-38 150
MS.gene010306.t1 AT1G30330 32.500 360 191 14 1 320 22 369 3.02e-38 150
MS.gene010306.t1 AT1G19850 31.768 362 197 13 1 323 40 390 3.06e-36 144
MS.gene010306.t1 AT1G19850 31.768 362 197 13 1 323 51 401 3.50e-36 144
MS.gene010306.t1 AT5G62000 30.612 343 198 10 4 316 61 393 3.78e-36 144
MS.gene010306.t1 AT5G62000 30.612 343 198 10 4 316 61 393 3.78e-36 144
MS.gene010306.t1 AT5G62000 30.612 343 198 10 4 316 61 393 3.78e-36 144
MS.gene010306.t1 AT5G62000 30.612 343 198 10 4 316 61 393 3.78e-36 144
MS.gene010306.t1 AT2G33860 32.796 372 196 17 4 338 54 408 4.41e-36 142
MS.gene010306.t1 AT5G62000 30.612 343 198 10 4 316 61 393 4.94e-36 144
MS.gene010306.t1 AT4G23980 29.365 378 214 13 4 349 12 368 7.16e-35 139
MS.gene010306.t1 AT4G23980 29.365 378 214 13 4 349 12 368 7.46e-35 139
MS.gene010306.t1 AT1G34390 31.657 338 198 11 1 316 24 350 2.62e-34 137
MS.gene010306.t1 AT1G35520 31.765 340 197 12 1 316 24 352 4.12e-34 136
MS.gene010306.t1 AT5G20730 31.594 345 199 13 1 316 21 357 1.23e-33 137
MS.gene010306.t1 AT5G20730 31.594 345 199 13 1 316 21 357 1.50e-33 136
MS.gene010306.t1 AT1G19220 31.609 348 194 13 1 316 20 355 1.79e-33 136
MS.gene010306.t1 AT1G34410 30.882 340 200 12 1 316 24 352 1.90e-33 135
MS.gene010306.t1 AT5G20730 31.686 344 199 13 1 316 21 356 2.38e-33 136
MS.gene010306.t1 AT1G35240 32.059 340 196 13 1 316 24 352 2.73e-33 134
MS.gene010306.t1 AT1G35540 30.890 382 222 14 1 355 24 390 2.88e-33 134
MS.gene010306.t1 AT1G34310 32.544 338 191 13 4 316 27 352 2.91e-33 134
MS.gene010306.t1 AT5G60450 31.707 369 206 14 3 335 40 398 5.90e-33 134
MS.gene010306.t1 AT5G60450 31.707 369 206 14 3 335 64 422 7.38e-33 134
MS.gene010306.t1 AT3G61830 33.238 349 188 15 39 362 13 341 3.14e-32 130
MS.gene010306.t1 AT1G34170 28.851 409 232 17 1 369 22 411 1.95e-29 122
MS.gene010306.t1 AT1G34170 28.070 399 222 15 1 369 22 385 2.22e-24 106
MS.gene010306.t1 AT1G34170 28.070 399 222 15 1 369 22 385 4.19e-24 106
MS.gene010306.t1 AT2G46530 32.700 263 140 12 103 343 36 283 4.30e-23 103
MS.gene010306.t1 AT2G33860 32.061 262 139 12 104 338 2 251 7.28e-22 99.0
MS.gene010306.t1 AT1G43950 33.990 203 118 7 1 191 24 222 7.73e-22 94.7
MS.gene010306.t1 AT5G37020 31.071 280 143 14 77 320 6 271 7.78e-22 100

Find 149 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCATTATCATAGGTTAAA+TGG 0.119220 5.4:-47408952 MS.gene010306:intron
TGTTTCCATCGTCGTCTTCT+TGG 0.207983 5.4:+47409988 None:intergenic
ATAGTTAAATTTGAAGATTC+AGG 0.238302 5.4:+47409471 None:intergenic
GGTGGTTTCTGCGGGGTATT+AGG 0.250113 5.4:+47408923 None:intergenic
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5.4:+47410215 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5.4:-47409413 MS.gene010306:CDS
ATGGAGATTGTGACACCATT+AGG 0.292749 5.4:+47410152 None:intergenic
TCTTGAGACTTCTGCAACTC+TGG 0.293011 5.4:+47409879 None:intergenic
CAGTTCTACAGGTGCGGACT+TGG 0.294646 5.4:-47408435 MS.gene010306:CDS
TCACTTGGTGCTTGGCTCAT+TGG 0.308376 5.4:+47408205 None:intergenic
ACCTTAAGCATGCGCCAATT+AGG 0.312806 5.4:+47409399 None:intergenic
CTTGGTCTGTTACGGGAGTT+AGG 0.314595 5.4:+47410036 None:intergenic
CTACTTCCCACAAGGTCATT+TGG 0.318755 5.4:-47410192 MS.gene010306:CDS
CGCAGAGTCGACTGATACTT+TGG 0.321532 5.4:-47408480 MS.gene010306:CDS
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5.4:-47409522 MS.gene010306:CDS
CAGTTCTAAAGGTGCGGACT+TGG 0.342634 5.4:-47408309 MS.gene010306:CDS
TCTTCAAATTTAACTATAAG+AGG 0.347983 5.4:-47409465 MS.gene010306:CDS
CCAAGCTTTAGTCTTGTATC+AGG 0.348766 5.4:+47408527 None:intergenic
TGACCAGGGGTGGTTTCTGC+GGG 0.350158 5.4:+47408915 None:intergenic
AGCGCATTTCTGCCATAGTT+TGG 0.354882 5.4:+47410256 None:intergenic
CAGTTCTAAAGGTGCGGACT+TGG 0.355336 5.4:-47408378 MS.gene010306:CDS
GATGAAAACAACGGAATCTT+CGG 0.357948 5.4:+47409749 None:intergenic
TTCTCACCTCTTTCAGCTGC+AGG 0.358959 5.4:+47409675 None:intergenic
CCCTAATTGGCGCATGCTTA+AGG 0.360706 5.4:-47409400 MS.gene010306:intron
CCAACCTTTAGCCTTGTATC+AGG 0.361804 5.4:+47408596 None:intergenic
GAGGGCAAAGCTTCGTTTGA+TGG 0.363032 5.4:-47409496 MS.gene010306:CDS
GTCTCTGTCACTGACCTTCC+AGG 0.363192 5.4:-47409855 MS.gene010306:CDS
AGTGGTACTTTGCTTGATAC+AGG 0.364586 5.4:-47408677 MS.gene010306:CDS
CCACAGGAGGAGGAGGTTCT+TGG 0.364923 5.4:+47410018 None:intergenic
GAAGAACTCTGAGAAAGTTT+AGG 0.368472 5.4:+47408824 None:intergenic
CCTTAAGCATGCGCCAATTA+GGG 0.368474 5.4:+47409400 None:intergenic
ATTAACTGAGAGTGCGGTCT+TGG 0.368633 5.4:-47409652 MS.gene010306:CDS
TGGAGATTGTGACACCATTA+GGG 0.370133 5.4:+47410153 None:intergenic
AGGAGGTTCTTGGTCTGTTA+CGG 0.370719 5.4:+47410028 None:intergenic
AGATTTCCAACCAATCTTCA+TGG 0.383662 5.4:+47409524 None:intergenic
AGTGATACTTTGGCTGATAC+AGG 0.384060 5.4:-47408344 MS.gene010306:CDS
ATGAAAACAACGGAATCTTC+GGG 0.384466 5.4:+47409750 None:intergenic
CTCATTATCATAGGTTAAAT+GGG 0.384717 5.4:-47408951 MS.gene010306:intron
GGCGCAGAGTCGAGTGATAC+AGG 0.389569 5.4:-47408413 MS.gene010306:CDS
ACACAAACTGATACTAAATC+TGG 0.395790 5.4:-47409939 MS.gene010306:CDS
GGAGGTTCTTGGTCTGTTAC+GGG 0.397553 5.4:+47410029 None:intergenic
CATGCATGTTCTTCCCCTAA+TGG 0.397590 5.4:-47410167 MS.gene010306:CDS
CAGTTCTAAAGGCGCTAACT+TGG 0.406148 5.4:-47408504 MS.gene010306:CDS
CTAAAGCTTGGCAGTTCTAC+AGG 0.408162 5.4:-47408446 MS.gene010306:CDS
GGTCTTGGGTGCAGTGGATT+TGG 0.411620 5.4:-47409637 MS.gene010306:CDS
TTTATCCCACAGATGGGAAT+TGG 0.411809 5.4:-47409584 MS.gene010306:CDS
TTGACCAGGGGTGGTTTCTG+CGG 0.423294 5.4:+47408914 None:intergenic
CTCCTATGGACTCCAAACTA+TGG 0.424679 5.4:-47410268 None:intergenic
GTGCTGAGTTGTTGCCTCAT+AGG 0.445159 5.4:-47408229 MS.gene010306:CDS
TACGGGAGTTAGGAGCAGTT+TGG 0.451525 5.4:+47410046 None:intergenic
CTAAAGCTTGGCAGTTCTAA+AGG 0.452082 5.4:-47408515 MS.gene010306:CDS
AACCACCCCTGGTCAATCCC+TGG 0.452665 5.4:-47408907 MS.gene010306:CDS
ATCTTTGGAATCTTGACGTC+GGG 0.453371 5.4:+47410230 None:intergenic
CTAAAGCTTGGCAGTTCTAA+AGG 0.454684 5.4:-47408251 MS.gene010306:CDS
CTAAAGCTTGGCAGTTCTAA+AGG 0.457123 5.4:-47408320 MS.gene010306:CDS
CTAAAGCTTGGCAGTTCTAA+AGG 0.457123 5.4:-47408389 MS.gene010306:CDS
CATCTGGCATGCAAGGAGTC+AGG 0.465776 5.4:-47408730 MS.gene010306:CDS
GGTAATGGTGTCAGTGTACT+TGG 0.473148 5.4:+47408773 None:intergenic
TTAACTGAGAGTGCGGTCTT+GGG 0.478339 5.4:-47409651 MS.gene010306:CDS
AACTTCCCTGCAGCTGAAAG+AGG 0.482726 5.4:-47409681 MS.gene010306:CDS
ATAAGCACTGGAGGTGGTAA+TGG 0.485821 5.4:+47408758 None:intergenic
CGCAGAGTCGTGTGATACTT+TGG 0.488987 5.4:-47408285 MS.gene010306:CDS
GTATAGGTGTAGACTTTGCC+TGG 0.494583 5.4:+47409837 None:intergenic
GTAGATGATGCCATGAAGAT+TGG 0.497274 5.4:-47409534 MS.gene010306:CDS
CATGGCATCATCTACAACCT+TGG 0.499201 5.4:+47409542 None:intergenic
AGTTCTAAAGGTGCGGACTT+GGG 0.500873 5.4:-47408308 MS.gene010306:CDS
AGTTCTACAGGTGCGGACTT+GGG 0.504327 5.4:-47408434 MS.gene010306:CDS
GTTGTACTTTGCCTGATACA+AGG 0.504571 5.4:-47408607 MS.gene010306:CDS
CGGGTAAACTTCAACCTCCC+AGG 0.506933 5.4:+47408889 None:intergenic
AAGATACCTCACTTGGTGCT+TGG 0.507669 5.4:+47408197 None:intergenic
CTTGATACAGGGCTAAAGCT+TGG 0.508063 5.4:-47408665 MS.gene010306:CDS
GGAGATGTTGACAGTGGAAG+AGG 0.510953 5.4:+47408859 None:intergenic
TCTTCTTGGCCGTGAACCAC+AGG 0.514079 5.4:+47410002 None:intergenic
TTTGCCTGATACAAGGCTAA+AGG 0.515810 5.4:-47408600 MS.gene010306:CDS
CCTCCTCCTCCTGTGGTTCA+CGG 0.519022 5.4:-47410011 MS.gene010306:CDS
GAAGGTGCTGACTTGTTCGC+AGG 0.520378 5.4:-47408704 MS.gene010306:CDS
AGTTCTAAAGGTGCGGACTT+GGG 0.527825 5.4:-47408377 MS.gene010306:CDS
GCTGATACAGTGCTAAAGCT+TGG 0.530738 5.4:-47408263 MS.gene010306:CDS
GCTGATACAGGGCTAAAGCT+TGG 0.532624 5.4:-47408332 MS.gene010306:CDS
AGGTGTAGACTTTGCCTGGA+AGG 0.536557 5.4:+47409841 None:intergenic
GCTGATACAGAGCTAAAGCT+TGG 0.537434 5.4:-47408458 MS.gene010306:CDS
TGGCCGTGAACCACAGGAGG+AGG 0.540091 5.4:+47410008 None:intergenic
CCATGCATCGTCTCTACTGT+TGG 0.543464 5.4:-47410104 MS.gene010306:CDS
AAATATATGTCGGAATTCGC+AGG 0.543716 5.4:-47409710 MS.gene010306:CDS
GAGACGATGCATGGAATGGA+TGG 0.546365 5.4:+47410113 None:intergenic
AGTTCTAAAGGCGCTAACTT+GGG 0.546463 5.4:-47408503 MS.gene010306:CDS
TTGGTTGGAAATCTGGAATG+AGG 0.547760 5.4:-47409515 MS.gene010306:CDS
TTTCTCAGAGTTCTTCTCCC+CGG 0.550822 5.4:-47408817 MS.gene010306:CDS
CTTGGCTCATTGGACCTATG+AGG 0.550878 5.4:+47408215 None:intergenic
TGCAGAGTCGAGTGATACTT+TGG 0.553312 5.4:-47408354 MS.gene010306:CDS
ATGATGCCATGAAGATTGGT+TGG 0.558711 5.4:-47409530 MS.gene010306:CDS
CAACCTCCCAGGGATTGACC+AGG 0.559879 5.4:+47408900 None:intergenic
AAACAGAGCAGACTAACTAA+AGG 0.559879 5.4:-47409807 MS.gene010306:CDS
CCGTGAACCACAGGAGGAGG+AGG 0.561880 5.4:+47410011 None:intergenic
CCTGATACAAGACTAAAGCT+TGG 0.563888 5.4:-47408527 MS.gene010306:CDS
GAGTGCGGTCTTGGGTGCAG+TGG 0.564030 5.4:-47409643 MS.gene010306:CDS
TGGACGGTAACAATCAAGAA+TGG 0.566778 5.4:+47410133 None:intergenic
ATGCAAGGAGTCAGGCAAGA+AGG 0.567091 5.4:-47408722 MS.gene010306:CDS
AAACTATGGCAGAAATGCGC+TGG 0.567239 5.4:-47410254 MS.gene010306:CDS
AGTGATACAGGGCTAAAGCT+TGG 0.568208 5.4:-47408401 MS.gene010306:CDS
ATTACACCAATTCCCATCTG+TGG 0.570163 5.4:+47409578 None:intergenic
TCCCAGGGATTGACCAGGGG+TGG 0.570982 5.4:+47408905 None:intergenic
AGTAGAGACGATGCATGGAA+TGG 0.572932 5.4:+47410109 None:intergenic
ACCACCCCTGGTCAATCCCT+GGG 0.574328 5.4:-47408906 MS.gene010306:CDS
GACTTGTTCGCAGGGTCGAG+TGG 0.575371 5.4:-47408695 MS.gene010306:CDS
TCTCACCTCTTTCAGCTGCA+GGG 0.578496 5.4:+47409676 None:intergenic
CTGACTCCTTGCATGCCAGA+TGG 0.584361 5.4:+47408731 None:intergenic
CATGTTCCAAATGACCTTGT+GGG 0.588081 5.4:+47410186 None:intergenic
TGGTTGGAAATCTGGAATGA+GGG 0.589287 5.4:-47409514 MS.gene010306:CDS
GTGGTACTTTGCTTGATACA+GGG 0.591580 5.4:-47408676 MS.gene010306:CDS
CCAAGAACCTCCTCCTCCTG+TGG 0.595254 5.4:-47410018 MS.gene010306:CDS
CCTGATACAAGGCTAAAGGT+TGG 0.595695 5.4:-47408596 MS.gene010306:CDS
TTACACCAATTCCCATCTGT+GGG 0.595749 5.4:+47409579 None:intergenic
GTGATACTTTGGCTGATACA+GGG 0.598282 5.4:-47408343 MS.gene010306:CDS
TTGTGTGAGAGTCTTGACAT+AGG 0.600208 5.4:+47409956 None:intergenic
TGCCATAGTTTGGAGTCCAT+AGG 0.605278 5.4:+47410266 None:intergenic
ATCAAGTCTGTGCATTGACG+AGG 0.606145 5.4:+47409906 None:intergenic
GCCAATGAAGATACCTCACT+TGG 0.608165 5.4:+47408190 None:intergenic
GCAAAGACCTCGTCGGTGTG+AGG 0.608714 5.4:+47410068 None:intergenic
GCGCAGAGTCGAGTGATACA+GGG 0.620334 5.4:-47408412 MS.gene010306:CDS
CGATGCATGGAATGGATGGA+CGG 0.620709 5.4:+47410117 None:intergenic
CAGTGGAAGAGGAAGTTGAC+GGG 0.623769 5.4:+47408870 None:intergenic
GGGTAAACTTCAACCTCCCA+GGG 0.625766 5.4:+47408890 None:intergenic
TGTTGTGGAGATGTTGACAG+TGG 0.633588 5.4:+47408853 None:intergenic
GCATGTTCCAAATGACCTTG+TGG 0.633704 5.4:+47410185 None:intergenic
AGAGCAGACTAACTAAAGGA+TGG 0.634232 5.4:-47409803 MS.gene010306:CDS
TACCCCGCAGAAACCACCCC+TGG 0.634765 5.4:-47408918 MS.gene010306:CDS
ACCTCCCAGGGATTGACCAG+GGG 0.638700 5.4:+47408902 None:intergenic
AACCTCCCAGGGATTGACCA+GGG 0.639624 5.4:+47408901 None:intergenic
AATCTTTGGAATCTTGACGT+CGG 0.641188 5.4:+47410229 None:intergenic
GACCAGGGGTGGTTTCTGCG+GGG 0.641988 5.4:+47408916 None:intergenic
AAGGTGCTGACTTGTTCGCA+GGG 0.642119 5.4:-47408703 MS.gene010306:CDS
CCAACAGTAGAGACGATGCA+TGG 0.649094 5.4:+47410104 None:intergenic
TCTTGGCCGTGAACCACAGG+AGG 0.650102 5.4:+47410005 None:intergenic
ACAGTGGAAGAGGAAGTTGA+CGG 0.650244 5.4:+47408869 None:intergenic
GCTTGGCAGTTCTAAAGGTG+CGG 0.654753 5.4:-47408315 MS.gene010306:CDS
GCTTGGCAGTTCTAAAGGTG+CGG 0.654753 5.4:-47408384 MS.gene010306:CDS
AATGAGCCAAGCACCAAGTG+AGG 0.655123 5.4:-47408203 None:intergenic
TGTTGATCCTCACACCGACG+AGG 0.655379 5.4:-47410075 MS.gene010306:CDS
GGATTTGGCTAACAAGAACA+TGG 0.659311 5.4:-47409622 MS.gene010306:CDS
GCTTGGCAGTTCTACAGGTG+CGG 0.659476 5.4:-47408441 MS.gene010306:CDS
GACTTGTTTGCAGAGTCGAG+TGG 0.663016 5.4:-47408557 MS.gene010306:CDS
CAGTTTGGCAAAGACCTCGT+CGG 0.663811 5.4:+47410061 None:intergenic
AAAGTTTACTACTTCCCACA+AGG 0.678948 5.4:-47410200 MS.gene010306:CDS
TGAGAAATTAACTGAGAGTG+CGG 0.683957 5.4:-47409658 MS.gene010306:CDS
GGAGATTGTGACACCATTAG+GGG 0.696103 5.4:+47410154 None:intergenic
TGAAAACAACGGAATCTTCG+GGG 0.698750 5.4:+47409751 None:intergenic
ACCCCTGGTCAATCCCTGGG+AGG 0.702615 5.4:-47408903 MS.gene010306:CDS
CACGGCCAAGAAGACGACGA+TGG 0.706758 5.4:-47409993 MS.gene010306:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATCTCATTATCAT+AGG - chr5.4:47409508-47409527 MS.gene010306:CDS 15.0%
!!! TAAATCAATCTTGTTTATTA+TGG + chr5.4:47409156-47409175 None:intergenic 15.0%
!!! TATGATAATGAGATAAAAAA+TGG + chr5.4:47409509-47409528 None:intergenic 15.0%
!! ATAGTTAAATTTGAAGATTC+AGG + chr5.4:47409000-47409019 None:intergenic 20.0%
!! TCTTCAAATTTAACTATAAG+AGG - chr5.4:47409003-47409022 MS.gene010306:intron 20.0%
!!! ATAAAATTTTGAGACTTAGT+AGG - chr5.4:47409217-47409236 MS.gene010306:intron 20.0%
! ACGTAATCTACTTACATTAA+CGG + chr5.4:47409352-47409371 None:intergenic 25.0%
! CTCATTATCATAGGTTAAAT+GGG - chr5.4:47409517-47409536 MS.gene010306:CDS 25.0%
! TCTCATTATCATAGGTTAAA+TGG - chr5.4:47409516-47409535 MS.gene010306:CDS 25.0%
!! AATTCCGACATATATTTTTC+CGG + chr5.4:47408755-47408774 None:intergenic 25.0%
!! AGAGTTTTTGATGAAAACAA+CGG + chr5.4:47408731-47408750 None:intergenic 25.0%
!! TTGAAGAATTTTGAATTTGC+CGG + chr5.4:47409673-47409692 None:intergenic 25.0%
!!! GCTTTCTTATTTTGTTCTAA+TGG - chr5.4:47409098-47409117 MS.gene010306:intron 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG + chr5.4:47408256-47408275 None:intergenic 25.0%
ACACAAACTGATACTAAATC+TGG - chr5.4:47408529-47408548 MS.gene010306:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG - chr5.4:47409055-47409074 MS.gene010306:intron 30.0%
CCAAAATAGAGATTCTACTT+AGG + chr5.4:47409392-47409411 None:intergenic 30.0%
GGAAATAATAATGTAGCAGA+TGG + chr5.4:47409308-47409327 None:intergenic 30.0%
! CCTAAGTAGAATCTCTATTT+TGG - chr5.4:47409389-47409408 MS.gene010306:intron 30.0%
! GAATTGGTGTAATTTTGTAG+TGG - chr5.4:47408900-47408919 MS.gene010306:CDS 30.0%
! TAACTATAAGAGGTTTTCAG+CGG - chr5.4:47409013-47409032 MS.gene010306:intron 30.0%
! TGAAGAATTTTGAATTTGCC+GGG + chr5.4:47409672-47409691 None:intergenic 30.0%
!! AAGTTTAGGACTTTTTGTTG+TGG + chr5.4:47409633-47409652 None:intergenic 30.0%
AAACAGAGCAGACTAACTAA+AGG - chr5.4:47408661-47408680 MS.gene010306:CDS 35.0%
AAAGTTTACTACTTCCCACA+AGG - chr5.4:47408268-47408287 MS.gene010306:CDS 35.0%
AAATATATGTCGGAATTCGC+AGG - chr5.4:47408758-47408777 MS.gene010306:CDS 35.0%
AGATTTCCAACCAATCTTCA+TGG + chr5.4:47408947-47408966 None:intergenic 35.0%
ATGAAAACAACGGAATCTTC+GGG + chr5.4:47408721-47408740 None:intergenic 35.0%
ATTAACGGTCATGAGATATC+AGG + chr5.4:47409337-47409356 None:intergenic 35.0%
GATGAAAACAACGGAATCTT+CGG + chr5.4:47408722-47408741 None:intergenic 35.0%
TCATGAGATATCAGGAACAA+TGG + chr5.4:47409329-47409348 None:intergenic 35.0%
TCTGCCGGAAAAATATATGT+CGG - chr5.4:47408748-47408767 MS.gene010306:CDS 35.0%
TGAGAAATTAACTGAGAGTG+CGG - chr5.4:47408810-47408829 MS.gene010306:CDS 35.0%
! AACTATAAGAGGTTTTCAGC+GGG - chr5.4:47409014-47409033 MS.gene010306:intron 35.0%
! ATGAAGATTGGTTGGAAATC+TGG - chr5.4:47408946-47408965 MS.gene010306:intron 35.0%
! GAAGAACTCTGAGAAAGTTT+AGG + chr5.4:47409647-47409666 None:intergenic 35.0%
! GAAGAATTTTGAATTTGCCG+GGG + chr5.4:47409671-47409690 None:intergenic 35.0%
! GTTTTCATCAAAAACTCTGC+CGG - chr5.4:47408733-47408752 MS.gene010306:CDS 35.0%
! TTGTGTTTTATCCCACAGAT+GGG - chr5.4:47408878-47408897 MS.gene010306:CDS 35.0%
!! AATCTTTGGAATCTTGACGT+CGG + chr5.4:47408242-47408261 None:intergenic 35.0%
!!! TAATTTTGTAGTGGATGCCA+AGG - chr5.4:47408909-47408928 MS.gene010306:CDS 35.0%
AGAGCAGACTAACTAAAGGA+TGG - chr5.4:47408665-47408684 MS.gene010306:CDS 40.0%
AGTGATACTTTGGCTGATAC+AGG - chr5.4:47410124-47410143 MS.gene010306:CDS 40.0%
AGTGGTACTTTGCTTGATAC+AGG - chr5.4:47409791-47409810 MS.gene010306:CDS 40.0%
ATGATGCCATGAAGATTGGT+TGG - chr5.4:47408938-47408957 MS.gene010306:CDS 40.0%
ATGGAGATTGTGACACCATT+AGG + chr5.4:47408319-47408338 None:intergenic 40.0%
ATTACACCAATTCCCATCTG+TGG + chr5.4:47408893-47408912 None:intergenic 40.0%
CATGTTCCAAATGACCTTGT+GGG + chr5.4:47408285-47408304 None:intergenic 40.0%
CCAGATGGAAAAATAAGCAC+TGG + chr5.4:47409725-47409744 None:intergenic 40.0%
CCTGATACAAGACTAAAGCT+TGG - chr5.4:47409941-47409960 MS.gene010306:CDS 40.0%
GGATTTGGCTAACAAGAACA+TGG - chr5.4:47408846-47408865 MS.gene010306:CDS 40.0%
GTAGATGATGCCATGAAGAT+TGG - chr5.4:47408934-47408953 MS.gene010306:CDS 40.0%
GTGATACTTTGGCTGATACA+GGG - chr5.4:47410125-47410144 MS.gene010306:CDS 40.0%
GTGGTACTTTGCTTGATACA+GGG - chr5.4:47409792-47409811 MS.gene010306:CDS 40.0%
GTTGTACTTTGCCTGATACA+AGG - chr5.4:47409861-47409880 MS.gene010306:CDS 40.0%
TGAAAACAACGGAATCTTCG+GGG + chr5.4:47408720-47408739 None:intergenic 40.0%
TGGACGGTAACAATCAAGAA+TGG + chr5.4:47408338-47408357 None:intergenic 40.0%
TGGAGATTGTGACACCATTA+GGG + chr5.4:47408318-47408337 None:intergenic 40.0%
TTACACCAATTCCCATCTGT+GGG + chr5.4:47408892-47408911 None:intergenic 40.0%
TTGTGTGAGAGTCTTGACAT+AGG + chr5.4:47408515-47408534 None:intergenic 40.0%
TTTATCCCACAGATGGGAAT+TGG - chr5.4:47408884-47408903 MS.gene010306:CDS 40.0%
TTTGCCTGATACAAGGCTAA+AGG - chr5.4:47409868-47409887 MS.gene010306:CDS 40.0%
! ATTTTTCCATCTGGCATGCA+AGG - chr5.4:47409731-47409750 MS.gene010306:CDS 40.0%
! CTAAAGCTTGGCAGTTCTAA+AGG - chr5.4:47409815-47409834 MS.gene010306:CDS 40.0%
! GTTGTGTTTTATCCCACAGA+TGG - chr5.4:47408877-47408896 MS.gene010306:CDS 40.0%
! TGGTTGGAAATCTGGAATGA+GGG - chr5.4:47408954-47408973 MS.gene010306:intron 40.0%
! TTGGTTGGAAATCTGGAATG+AGG - chr5.4:47408953-47408972 MS.gene010306:intron 40.0%
!! AGTTCTAAAGGCGCTAACTT+GGG - chr5.4:47409965-47409984 MS.gene010306:CDS 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG + chr5.4:47408241-47408260 None:intergenic 40.0%
!! CCAAGCTTTAGTCTTGTATC+AGG + chr5.4:47409944-47409963 None:intergenic 40.0%
!! CCAGTGCTTATTTTTCCATC+TGG - chr5.4:47409722-47409741 MS.gene010306:CDS 40.0%
!! GATGGAAAAATAAGCACTGG+AGG + chr5.4:47409722-47409741 None:intergenic 40.0%
!! GCTCTGTTTTGAGTAGGTAT+AGG + chr5.4:47408650-47408669 None:intergenic 40.0%
!! TAGTCTGCTCTGTTTTGAGT+AGG + chr5.4:47408656-47408675 None:intergenic 40.0%
AAACTATGGCAGAAATGCGC+TGG - chr5.4:47408214-47408233 MS.gene010306:CDS 45.0%
ACAGTGGAAGAGGAAGTTGA+CGG + chr5.4:47409602-47409621 None:intergenic 45.0%
ACCTTAAGCATGCGCCAATT+AGG + chr5.4:47409072-47409091 None:intergenic 45.0%
AGCGCATTTCTGCCATAGTT+TGG + chr5.4:47408215-47408234 None:intergenic 45.0%
AGGAGGTTCTTGGTCTGTTA+CGG + chr5.4:47408443-47408462 None:intergenic 45.0%
AGTAGAGACGATGCATGGAA+TGG + chr5.4:47408362-47408381 None:intergenic 45.0%
AGTGATACAGGGCTAAAGCT+TGG - chr5.4:47410067-47410086 MS.gene010306:CDS 45.0%
CATGCATGTTCTTCCCCTAA+TGG - chr5.4:47408301-47408320 MS.gene010306:CDS 45.0%
CATGGCATCATCTACAACCT+TGG + chr5.4:47408929-47408948 None:intergenic 45.0%
CCTGATACAAGGCTAAAGGT+TGG - chr5.4:47409872-47409891 MS.gene010306:CDS 45.0%
CCTTAAGCATGCGCCAATTA+GGG + chr5.4:47409071-47409090 None:intergenic 45.0%
CTAAAGCTTGGCAGTTCTAC+AGG - chr5.4:47410022-47410041 MS.gene010306:CDS 45.0%
CTACTTCCCACAAGGTCATT+TGG - chr5.4:47408276-47408295 MS.gene010306:CDS 45.0%
CTTGATACAGGGCTAAAGCT+TGG - chr5.4:47409803-47409822 MS.gene010306:CDS 45.0%
GCATGTTCCAAATGACCTTG+TGG + chr5.4:47408286-47408305 None:intergenic 45.0%
GCTGATACAGAGCTAAAGCT+TGG - chr5.4:47410010-47410029 MS.gene010306:CDS 45.0%
GCTGATACAGTGCTAAAGCT+TGG - chr5.4:47410205-47410224 MS.gene010306:CDS 45.0%
GGAGATTGTGACACCATTAG+GGG + chr5.4:47408317-47408336 None:intergenic 45.0%
GTATAGGTGTAGACTTTGCC+TGG + chr5.4:47408634-47408653 None:intergenic 45.0%
TCTTGAGACTTCTGCAACTC+TGG + chr5.4:47408592-47408611 None:intergenic 45.0%
TGCAGAGTCGAGTGATACTT+TGG - chr5.4:47410114-47410133 MS.gene010306:CDS 45.0%
TGTTGTGGAGATGTTGACAG+TGG + chr5.4:47409618-47409637 None:intergenic 45.0%
TGTTTCCATCGTCGTCTTCT+TGG + chr5.4:47408483-47408502 None:intergenic 45.0%
TTTCTCAGAGTTCTTCTCCC+CGG - chr5.4:47409651-47409670 MS.gene010306:CDS 45.0%
! ATAAGAGGTTTTCAGCGGGA+GGG - chr5.4:47409018-47409037 MS.gene010306:intron 45.0%
! ATCAAGTCTGTGCATTGACG+AGG + chr5.4:47408565-47408584 None:intergenic 45.0%
! ATTAACTGAGAGTGCGGTCT+TGG - chr5.4:47408816-47408835 MS.gene010306:CDS 45.0%
! GGTAATGGTGTCAGTGTACT+TGG + chr5.4:47409698-47409717 None:intergenic 45.0%
! TATAAGAGGTTTTCAGCGGG+AGG - chr5.4:47409017-47409036 MS.gene010306:intron 45.0%
! TTAACTGAGAGTGCGGTCTT+GGG - chr5.4:47408817-47408836 MS.gene010306:CDS 45.0%
!! AGTTCTAAAGGTGCGGACTT+GGG - chr5.4:47410091-47410110 MS.gene010306:CDS 45.0%
!! ATAAGCACTGGAGGTGGTAA+TGG + chr5.4:47409713-47409732 None:intergenic 45.0%
!! CAGTTCTAAAGGCGCTAACT+TGG - chr5.4:47409964-47409983 MS.gene010306:CDS 45.0%
!! CCAACCTTTAGCCTTGTATC+AGG + chr5.4:47409875-47409894 None:intergenic 45.0%
!! GGAAAAATAAGCACTGGAGG+TGG + chr5.4:47409719-47409738 None:intergenic 45.0%
AACTTCCCTGCAGCTGAAAG+AGG - chr5.4:47408787-47408806 MS.gene010306:CDS 50.0%
AGGTGTAGACTTTGCCTGGA+AGG + chr5.4:47408630-47408649 None:intergenic 50.0%
AGTTCTACAGGTGCGGACTT+GGG - chr5.4:47410034-47410053 MS.gene010306:CDS 50.0%
ATGCAAGGAGTCAGGCAAGA+AGG - chr5.4:47409746-47409765 MS.gene010306:CDS 50.0%
CAGTGGAAGAGGAAGTTGAC+GGG + chr5.4:47409601-47409620 None:intergenic 50.0%
CAGTTTGGCAAAGACCTCGT+CGG + chr5.4:47408410-47408429 None:intergenic 50.0%
CCAACAGTAGAGACGATGCA+TGG + chr5.4:47408367-47408386 None:intergenic 50.0%
CCATGCATCGTCTCTACTGT+TGG - chr5.4:47408364-47408383 MS.gene010306:CDS 50.0%
CCCTAATTGGCGCATGCTTA+AGG - chr5.4:47409068-47409087 MS.gene010306:intron 50.0%
CGATGCATGGAATGGATGGA+CGG + chr5.4:47408354-47408373 None:intergenic 50.0%
CGCAGAGTCGACTGATACTT+TGG - chr5.4:47409988-47410007 MS.gene010306:CDS 50.0%
CGCAGAGTCGTGTGATACTT+TGG - chr5.4:47410183-47410202 MS.gene010306:CDS 50.0%
CTTGGCTCATTGGACCTATG+AGG + chr5.4:47410256-47410275 None:intergenic 50.0%
CTTGGTCTGTTACGGGAGTT+AGG + chr5.4:47408435-47408454 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG + chr5.4:47408358-47408377 None:intergenic 50.0%
GAGGGCAAAGCTTCGTTTGA+TGG - chr5.4:47408972-47408991 MS.gene010306:intron 50.0%
GCTGATACAGGGCTAAAGCT+TGG - chr5.4:47410136-47410155 MS.gene010306:CDS 50.0%
GGAGATGTTGACAGTGGAAG+AGG + chr5.4:47409612-47409631 None:intergenic 50.0%
GGAGGTTCTTGGTCTGTTAC+GGG + chr5.4:47408442-47408461 None:intergenic 50.0%
GGGTAAACTTCAACCTCCCA+GGG + chr5.4:47409581-47409600 None:intergenic 50.0%
GTGCTGAGTTGTTGCCTCAT+AGG - chr5.4:47410239-47410258 MS.gene010306:CDS 50.0%
TACGGGAGTTAGGAGCAGTT+TGG + chr5.4:47408425-47408444 None:intergenic 50.0%
TCTCACCTCTTTCAGCTGCA+GGG + chr5.4:47408795-47408814 None:intergenic 50.0%
TTCTCACCTCTTTCAGCTGC+AGG + chr5.4:47408796-47408815 None:intergenic 50.0%
! GACTTGTTTGCAGAGTCGAG+TGG - chr5.4:47409911-47409930 MS.gene010306:CDS 50.0%
! TAAGAGGTTTTCAGCGGGAG+GGG - chr5.4:47409019-47409038 MS.gene010306:intron 50.0%
!! AAGGTGCTGACTTGTTCGCA+GGG - chr5.4:47409765-47409784 MS.gene010306:CDS 50.0%
!! CAGTTCTAAAGGTGCGGACT+TGG - chr5.4:47410090-47410109 MS.gene010306:CDS 50.0%
!! GCTTGGCAGTTCTAAAGGTG+CGG - chr5.4:47410084-47410103 MS.gene010306:CDS 50.0%
!! TCACTTGGTGCTTGGCTCAT+TGG + chr5.4:47410266-47410285 None:intergenic 50.0%
AACCTCCCAGGGATTGACCA+GGG + chr5.4:47409570-47409589 None:intergenic 55.0%
CAGTTCTACAGGTGCGGACT+TGG - chr5.4:47410033-47410052 MS.gene010306:CDS 55.0%
CATCTGGCATGCAAGGAGTC+AGG - chr5.4:47409738-47409757 MS.gene010306:CDS 55.0%
CGGGTAAACTTCAACCTCCC+AGG + chr5.4:47409582-47409601 None:intergenic 55.0%
CTGACTCCTTGCATGCCAGA+TGG + chr5.4:47409740-47409759 None:intergenic 55.0%
GCGCAGAGTCGAGTGATACA+GGG - chr5.4:47410056-47410075 MS.gene010306:CDS 55.0%
GCTTGGCAGTTCTACAGGTG+CGG - chr5.4:47410027-47410046 MS.gene010306:CDS 55.0%
GTCTCTGTCACTGACCTTCC+AGG - chr5.4:47408613-47408632 MS.gene010306:CDS 55.0%
TCTTCTTGGCCGTGAACCAC+AGG + chr5.4:47408469-47408488 None:intergenic 55.0%
TGTTGATCCTCACACCGACG+AGG - chr5.4:47408393-47408412 MS.gene010306:CDS 55.0%
TTGACCAGGGGTGGTTTCTG+CGG + chr5.4:47409557-47409576 None:intergenic 55.0%
! GGTCTTGGGTGCAGTGGATT+TGG - chr5.4:47408831-47408850 MS.gene010306:CDS 55.0%
! GGTGGTTTCTGCGGGGTATT+AGG + chr5.4:47409548-47409567 None:intergenic 55.0%
!! GAAGGTGCTGACTTGTTCGC+AGG - chr5.4:47409764-47409783 MS.gene010306:CDS 55.0%
AACCACCCCTGGTCAATCCC+TGG - chr5.4:47409561-47409580 MS.gene010306:CDS 60.0%
ACCACCCCTGGTCAATCCCT+GGG - chr5.4:47409562-47409581 MS.gene010306:CDS 60.0%
ACCTCCCAGGGATTGACCAG+GGG + chr5.4:47409569-47409588 None:intergenic 60.0%
CAACCTCCCAGGGATTGACC+AGG + chr5.4:47409571-47409590 None:intergenic 60.0%
CACGGCCAAGAAGACGACGA+TGG - chr5.4:47408475-47408494 MS.gene010306:CDS 60.0%
CCAAGAACCTCCTCCTCCTG+TGG - chr5.4:47408450-47408469 MS.gene010306:CDS 60.0%
CCACAGGAGGAGGAGGTTCT+TGG + chr5.4:47408453-47408472 None:intergenic 60.0%
CCTCCTCCTCCTGTGGTTCA+CGG - chr5.4:47408457-47408476 MS.gene010306:CDS 60.0%
GCAAAGACCTCGTCGGTGTG+AGG + chr5.4:47408403-47408422 None:intergenic 60.0%
GGCGCAGAGTCGAGTGATAC+AGG - chr5.4:47410055-47410074 MS.gene010306:CDS 60.0%
TCTTGGCCGTGAACCACAGG+AGG + chr5.4:47408466-47408485 None:intergenic 60.0%
TGACCAGGGGTGGTTTCTGC+GGG + chr5.4:47409556-47409575 None:intergenic 60.0%
! GACTTGTTCGCAGGGTCGAG+TGG - chr5.4:47409773-47409792 MS.gene010306:CDS 60.0%
ACCCCTGGTCAATCCCTGGG+AGG - chr5.4:47409565-47409584 MS.gene010306:CDS 65.0%
CCGTGAACCACAGGAGGAGG+AGG + chr5.4:47408460-47408479 None:intergenic 65.0%
GACCAGGGGTGGTTTCTGCG+GGG + chr5.4:47409555-47409574 None:intergenic 65.0%
GAGTGCGGTCTTGGGTGCAG+TGG - chr5.4:47408825-47408844 MS.gene010306:CDS 65.0%
TACCCCGCAGAAACCACCCC+TGG - chr5.4:47409550-47409569 MS.gene010306:CDS 65.0%
TCCCAGGGATTGACCAGGGG+TGG + chr5.4:47409566-47409585 None:intergenic 65.0%
TGGCCGTGAACCACAGGAGG+AGG + chr5.4:47408463-47408482 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 47408205 47410285 47408205 ID=MS.gene010306
chr5.4 mRNA 47408205 47410285 47408205 ID=MS.gene010306.t1;Parent=MS.gene010306
chr5.4 exon 47409401 47410285 47409401 ID=MS.gene010306.t1.exon1;Parent=MS.gene010306.t1
chr5.4 CDS 47409401 47410285 47409401 ID=cds.MS.gene010306.t1;Parent=MS.gene010306.t1
chr5.4 exon 47408205 47408960 47408205 ID=MS.gene010306.t1.exon2;Parent=MS.gene010306.t1
chr5.4 CDS 47408205 47408960 47408205 ID=cds.MS.gene010306.t1;Parent=MS.gene010306.t1
Gene Sequence

>MS.gene010306

ATGGACTCCAAACTATGGCAGAAATGCGCTGGTCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACAAGGTCATTTGGAACATGCATGTTCTTCCCCTAATGGTGTCACAATCTCCATTCTTGATTGTTACCGTCCATCCATTCCATGCATCGTCTCTACTGTTGGTCTCTTTGTTGATCCTCACACCGACGAGGTCTTTGCCAAACTGCTCCTAACTCCCGTAACAGACCAAGAACCTCCTCCTCCTGTGGTTCACGGCCAAGAAGACGACGATGGAAACAATCTTGTTTCCTATGTCAAGACTCTCACACAAACTGATACTAAATCTGGATTCAATATGCCTCGTCAATGCACAGACTTGATCTTTCCAGAGTTGCAGAAGTCTCAAGAAGTCTCTGTCACTGACCTTCCAGGCAAAGTCTACACCTATACCTACTCAAAACAGAGCAGACTAACTAAAGGATGGTTTAATTTTGTAAGTGAGAAGAAACTCGTCCCCGAAGATTCCGTTGTTTTCATCAAAAACTCTGCCGGAAAAATATATGTCGGAATTCGCAGGAACACTAACTTCCCTGCAGCTGAAAGAGGTGAGAAATTAACTGAGAGTGCGGTCTTGGGTGCAGTGGATTTGGCTAACAAGAACATGGCGTTTGAAGTTGTGTTTTATCCCACAGATGGGAATTGGTGTAATTTTGTAGTGGATGCCAAGGTTGTAGATGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGGGCAAAGCTTCGTTTGATGGAACCTGAATCTTCAAATTTAACTATAAGAGGTTTTCAGCGGGAGGGGACAATCTCTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTAAGGTACTTATGCTTTCTTATTTTGTTCTAATGGTATTTCAATTGCATATTGTTATCAAGTTGAATCCATAATAAACAAGATTGATTTAGATATTAAAGCTATAATTAGATGATTCTTGATGAAATAGATATAAAATTTTGAGACTTAGTAGGTTGCTACTGTTTTGAATGTCAAATGTAATTCTAAAATTAAGAAACACAAAATCATTACAGAATATCCATCTGCTACATTATTATTTCCATTGTTCCTGATATCTCATGACCGTTAATGTAAGTAGATTACGTTTTAGAATCATATCACACCTAAGTAGAATCTCTATTTTGGCTCATGTTATTAATTGTTATGAAACTTATTAATTGATGTCTAAATACATATATATACTAAGTTATCAAATATTTCAAATTATTGATATTGATTTCCATTTTTTATCTCATTATCATAGGTTAAATGGGATGAACCTAATACCCCGCAGAAACCACCCCTGGTCAATCCCTGGGAGGTTGAAGTTTACCCGTCAACTTCCTCTTCCACTGTCAACATCTCCACAACAAAAAGTCCTAAACTTTCTCAGAGTTCTTCTCCCCGGCAAATTCAAAATTCTTCAATGCCAAGTACACTGACACCATTACCACCTCCAGTGCTTATTTTTCCATCTGGCATGCAAGGAGTCAGGCAAGAAGGTGCTGACTTGTTCGCAGGGTCGAGTGGTACTTTGCTTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGATGCTGAATTGTTCGCAGAGTTGAGTTGTACTTTGCCTGATACAAGGCTAAAGGTTGGCAGTTCTAAAGATGTTGACTTGTTTGCAGAGTCGAGTGGTACTTTGCCTGATACAAGACTAAAGCTTGGCAGTTCTAAAGGCGCTAACTTGGGCGCAGAGTCGACTGATACTTTGGCTGATACAGAGCTAAAGCTTGGCAGTTCTACAGGTGCGGACTTGGGCGCAGAGTCGAGTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGGTGCGGACTTGGGTGCAGAGTCGAGTGATACTTTGGCTGATACAGGGCTAAAGCTTGGCAGTTCTAAAGGTGCGGACTTGGGCGCAGAGTCGTGTGATACTTTGGCTGATACAGTGCTAAAGCTTGGCAGTTCTAAAGGTGCTGAGTTGTTGCCTCATAGGTCCAATGAGCCAAGCACCAAGTGA
Protein sequence

>MS.gene010306.t1

MDSKLWQKCAGPDVKIPKIHSKVYYFPQGHLEHACSSPNGVTISILDCYRPSIPCIVSTVGLFVDPHTDEVFAKLLLTPVTDQEPPPPVVHGQEDDDGNNLVSYVKTLTQTDTKSGFNMPRQCTDLIFPELQKSQEVSVTDLPGKVYTYTYSKQSRLTKGWFNFVSEKKLVPEDSVVFIKNSAGKIYVGIRRNTNFPAAERGEKLTESAVLGAVDLANKNMAFEVVFYPTDGNWCNFVVDAKVVDDAMKIGWKSGMRAKLRLMEPESSNLTIRGFQREGTISNDSSNVPNWRMLKVKWDEPNTPQKPPLVNPWEVEVYPSTSSSTVNISTTKSPKLSQSSSPRQIQNSSMPSTLTPLPPPVLIFPSGMQGVRQEGADLFAGSSGTLLDTGLKLGSSKDAELFAELSCTLPDTRLKVGSSKDVDLFAESSGTLPDTRLKLGSSKGANLGAESTDTLADTELKLGSSTGADLGAESSDTGLKLGSSKGADLGAESSDTLADTGLKLGSSKGADLGAESCDTLADTVLKLGSSKGAELLPHRSNEPSTK