AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010309

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010309.t1 MTR_5g061890 90.099 404 32 2 1 401 1 399 0.0 736
MS.gene010309.t1 MTR_5g060780 89.109 404 36 2 1 401 1 399 0.0 729
MS.gene010309.t1 MTR_5g460920 89.109 404 36 2 1 401 1 399 0.0 727
MS.gene010309.t1 MTR_5g060630 54.676 417 136 8 1 367 1 414 6.61e-131 386
MS.gene010309.t1 MTR_5g460740 54.676 417 136 8 1 367 1 414 6.61e-131 386
MS.gene010309.t1 MTR_5g061220 53.418 395 143 9 1 364 8 392 1.28e-126 375
MS.gene010309.t1 MTR_1g058210 53.281 381 142 10 1 364 1 362 1.84e-117 350
MS.gene010309.t1 MTR_2g024430 48.995 398 167 16 1 382 9 386 7.41e-104 317
MS.gene010309.t1 MTR_5g040880 49.408 338 148 9 8 335 7 331 9.60e-95 293
MS.gene010309.t1 MTR_5g040740 51.205 332 127 11 1 315 1 314 1.62e-88 274
MS.gene010309.t1 MTR_2g006270 44.416 385 181 14 6 374 7 374 1.00e-87 273
MS.gene010309.t1 MTR_5g082140 45.376 346 160 10 6 336 6 337 2.96e-84 265
MS.gene010309.t1 MTR_2g006380 42.520 381 187 14 6 374 14 374 3.15e-84 264
MS.gene010309.t1 MTR_5g060770 52.117 307 91 7 61 365 7 259 6.98e-80 250
MS.gene010309.t1 MTR_5g062970 57.143 196 68 7 12 195 38 229 1.75e-63 204
MS.gene010309.t1 MTR_3g073420 35.714 378 181 11 9 336 16 381 2.61e-62 211
MS.gene010309.t1 MTR_5g460910 40.816 392 121 12 52 401 14 336 5.56e-61 200
MS.gene010309.t1 MTR_1g064430 36.253 411 191 14 9 360 37 435 3.43e-57 199
MS.gene010309.t1 MTR_1g064430 36.253 411 191 14 9 360 24 422 3.69e-57 199
MS.gene010309.t1 MTR_1g094960 35.294 408 192 16 9 361 16 406 4.23e-57 197
MS.gene010309.t1 MTR_2g094570 34.565 379 187 12 9 336 8 376 3.78e-53 187
MS.gene010309.t1 MTR_2g094570 34.565 379 187 12 9 336 8 376 4.12e-53 188
MS.gene010309.t1 MTR_4g058930 35.641 390 183 13 9 340 9 388 7.56e-53 187
MS.gene010309.t1 MTR_2g094570 34.565 379 187 12 9 336 64 432 9.85e-53 187
MS.gene010309.t1 MTR_4g058930 35.641 390 183 13 9 340 9 388 1.30e-52 186
MS.gene010309.t1 MTR_7g101280 30.769 403 192 14 9 341 28 413 5.09e-48 173
MS.gene010309.t1 MTR_0041s0110 30.769 403 192 14 9 341 28 413 5.09e-48 173
MS.gene010309.t1 MTR_5g062960 63.393 112 40 1 227 338 1 111 1.53e-39 140
MS.gene010309.t1 MTR_2g014770 29.457 387 231 15 4 364 43 413 4.96e-39 148
MS.gene010309.t1 MTR_2g014770 29.457 387 231 15 4 364 43 413 8.47e-39 147
MS.gene010309.t1 MTR_7g101275 32.698 315 174 12 22 314 2 300 8.80e-39 144
MS.gene010309.t1 MTR_2g005240 28.497 386 231 10 12 371 23 389 2.54e-37 143
MS.gene010309.t1 MTR_4g021580 28.412 359 237 8 12 358 19 369 1.91e-36 140
MS.gene010309.t1 MTR_4g021580 28.255 361 239 8 10 358 17 369 2.56e-36 140
MS.gene010309.t1 MTR_8g100050 29.290 338 220 8 12 335 59 391 3.26e-36 140
MS.gene010309.t1 MTR_7g062540 28.453 362 236 10 12 359 14 366 7.08e-36 139
MS.gene010309.t1 MTR_8g100050 29.290 338 220 8 12 335 59 391 8.82e-36 139
MS.gene010309.t1 MTR_0041s0120 31.481 324 178 12 22 314 2 312 1.20e-34 133
MS.gene010309.t1 MTR_4g088210 28.756 386 228 15 12 369 38 404 2.73e-34 135
MS.gene010309.t1 MTR_4g088210 28.756 386 228 15 12 369 38 404 2.77e-34 135
MS.gene010309.t1 MTR_2g018690 29.651 344 219 10 9 335 20 357 3.21e-34 135
MS.gene010309.t1 MTR_8g027440 28.750 400 259 12 12 398 29 415 2.14e-33 132
MS.gene010309.t1 MTR_4g124900 28.571 350 213 10 9 335 20 355 5.20e-32 129
MS.gene010309.t1 MTR_4g124900 28.571 350 213 10 9 335 20 355 5.29e-32 129
MS.gene010309.t1 MTR_5g076270 29.326 341 218 10 12 335 24 358 9.35e-32 127
MS.gene010309.t1 MTR_2g043250 28.940 349 213 12 9 335 20 355 1.33e-31 127
MS.gene010309.t1 MTR_1g024025 29.912 341 215 11 12 335 43 376 1.54e-31 127
MS.gene010309.t1 MTR_8g079492 28.249 354 210 12 9 335 20 356 6.13e-31 125
MS.gene010309.t1 MTR_8g079492 28.249 354 210 12 9 335 20 356 6.19e-31 125
MS.gene010309.t1 MTR_3g064050 29.032 341 219 10 12 335 26 360 1.30e-30 124
MS.gene010309.t1 MTR_2g093740 29.219 397 235 16 12 376 56 438 4.38e-29 120
MS.gene010309.t1 MTR_2g093740 29.219 397 235 16 12 376 56 438 4.69e-29 119
MS.gene010309.t1 MTR_2g093740 29.219 397 235 16 12 376 56 438 6.26e-29 119
MS.gene010309.t1 MTR_8g101360 28.818 347 218 11 9 335 28 365 1.46e-27 115
MS.gene010309.t1 MTR_4g060460 27.518 407 230 15 12 377 54 436 2.06e-27 115
MS.gene010309.t1 MTR_2g093740 28.780 410 233 17 12 376 56 451 4.10e-27 114
MS.gene010309.t1 MTR_2g093740 28.780 410 233 17 12 376 56 451 5.29e-27 113
MS.gene010309.t1 MTR_2g093740 28.780 410 233 17 12 376 56 451 5.46e-27 114
MS.gene010309.t1 MTR_4g060460 27.182 401 227 15 12 371 54 430 3.98e-26 111
MS.gene010309.t1 MTR_8g079492 26.829 287 176 9 70 335 1 274 2.59e-19 90.5
MS.gene010309.t1 MTR_8g446900 26.846 298 174 12 61 335 12 288 1.64e-18 86.7
MS.gene010309.t1 MTR_2g093740 28.470 281 175 11 112 376 38 308 5.66e-15 77.0
MS.gene010309.t1 MTR_2g093740 28.470 281 175 11 112 376 38 308 6.02e-15 77.0
MS.gene010309.t1 MTR_5g046120 35.897 117 69 3 50 160 12 128 1.07e-13 68.2
MS.gene010309.t1 MTR_2g093740 27.891 294 173 12 112 376 38 321 4.29e-13 71.2
MS.gene010309.t1 MTR_2g093740 27.891 294 173 12 112 376 38 321 4.77e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010309.t1 AT1G77850 35.027 374 188 13 8 336 15 378 6.89e-62 209
MS.gene010309.t1 AT1G77850 35.027 374 188 13 8 336 15 378 9.00e-62 209
MS.gene010309.t1 AT4G30080 35.550 391 191 14 9 347 16 397 1.39e-52 186
MS.gene010309.t1 AT2G28350 33.249 394 188 14 9 340 7 387 1.00e-49 178
MS.gene010309.t1 AT2G28350 33.249 394 188 14 9 340 7 387 1.06e-49 178
MS.gene010309.t1 AT2G46530 30.055 366 230 8 12 361 42 397 1.25e-42 158
MS.gene010309.t1 AT2G46530 30.055 366 230 8 12 361 21 376 1.33e-42 157
MS.gene010309.t1 AT3G61830 30.571 350 211 11 12 342 25 361 6.57e-42 155
MS.gene010309.t1 AT1G59750 31.948 385 228 14 12 377 22 391 1.23e-39 150
MS.gene010309.t1 AT1G59750 31.948 385 228 14 12 377 22 391 1.23e-39 150
MS.gene010309.t1 AT1G59750 32.115 383 226 14 12 375 22 389 1.92e-39 149
MS.gene010309.t1 AT1G34390 30.576 399 233 14 12 385 27 406 2.41e-39 149
MS.gene010309.t1 AT1G35520 31.463 410 219 17 12 386 27 409 3.49e-39 148
MS.gene010309.t1 AT1G59750 31.967 366 218 13 12 358 22 375 6.12e-39 148
MS.gene010309.t1 AT1G34410 30.467 407 225 17 12 385 27 408 2.61e-37 143
MS.gene010309.t1 AT1G34310 30.729 384 213 15 9 364 24 382 2.65e-36 140
MS.gene010309.t1 AT1G35540 30.882 408 222 19 12 385 27 408 3.47e-36 140
MS.gene010309.t1 AT5G62000 29.107 347 209 10 12 335 61 393 1.43e-35 139
MS.gene010309.t1 AT5G62000 29.107 347 209 10 12 335 61 393 1.47e-35 139
MS.gene010309.t1 AT5G62000 29.107 347 209 10 12 335 61 393 1.47e-35 139
MS.gene010309.t1 AT5G62000 29.107 347 209 10 12 335 61 393 1.47e-35 139
MS.gene010309.t1 AT5G62000 29.107 347 209 10 12 335 61 393 1.47e-35 139
MS.gene010309.t1 AT1G35240 30.579 363 196 15 12 343 27 364 2.05e-34 134
MS.gene010309.t1 AT4G23980 28.612 353 215 11 12 340 12 351 4.41e-34 134
MS.gene010309.t1 AT4G23980 28.612 353 215 11 12 340 12 351 4.46e-34 134
MS.gene010309.t1 AT5G37020 30.409 342 213 12 12 335 22 356 4.75e-34 134
MS.gene010309.t1 AT5G37020 30.409 342 213 12 12 335 22 356 5.52e-34 134
MS.gene010309.t1 AT1G30330 29.412 340 219 9 12 335 25 359 4.25e-33 132
MS.gene010309.t1 AT1G30330 29.412 340 219 9 12 335 25 359 4.25e-33 132
MS.gene010309.t1 AT1G30330 29.412 340 219 9 12 335 23 357 4.49e-33 132
MS.gene010309.t1 AT1G34170 29.805 359 213 13 12 349 25 365 1.42e-32 128
MS.gene010309.t1 AT2G33860 28.771 358 218 12 12 347 54 396 3.05e-32 128
MS.gene010309.t1 AT1G19220 28.571 350 213 11 9 335 20 355 4.12e-31 126
MS.gene010309.t1 AT1G19850 30.398 352 212 11 7 336 49 389 2.68e-30 123
MS.gene010309.t1 AT1G19850 30.398 352 212 11 7 336 38 378 2.90e-30 123
MS.gene010309.t1 AT5G20730 29.429 350 211 12 9 335 21 357 3.94e-30 123
MS.gene010309.t1 AT5G20730 29.429 350 211 12 9 335 21 357 4.15e-30 123
MS.gene010309.t1 AT5G20730 28.977 352 209 11 9 335 21 356 1.06e-29 122
MS.gene010309.t1 AT3G61830 30.435 299 181 9 59 342 24 310 1.75e-29 120
MS.gene010309.t1 AT1G43950 32.353 204 118 6 12 203 27 222 1.01e-26 107
MS.gene010309.t1 AT1G34170 27.809 356 198 12 12 349 25 339 2.72e-26 110
MS.gene010309.t1 AT1G34170 27.809 356 198 12 12 349 25 339 3.72e-26 110
MS.gene010309.t1 AT5G60450 28.490 351 214 13 12 335 65 405 4.23e-26 111
MS.gene010309.t1 AT5G60450 28.490 351 214 13 12 335 41 381 4.42e-26 110
MS.gene010309.t1 AT2G46530 29.119 261 168 6 111 361 36 289 3.08e-23 102
MS.gene010309.t1 AT2G33860 29.032 248 154 9 112 347 2 239 1.44e-18 87.8
MS.gene010309.t1 AT5G37020 28.839 267 170 10 83 335 1 261 3.58e-17 84.0

Find 105 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGCATATCTGCCATATTT+TGG 0.143600 5.4:-47386361 None:intergenic
ACCAAGTGAGATATCTTTAT+TGG 0.193551 5.4:+47387973 MS.gene010309:CDS
AACTGATGAGATTAAGGTTT+TGG 0.252605 5.4:+47386989 MS.gene010309:CDS
TGTATCCAACATCGTCTTCT+TGG 0.259399 5.4:-47386629 None:intergenic
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5.4:-47386402 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5.4:+47387237 MS.gene010309:CDS
GATGACAATTTCTCAGCTTA+AGG 0.289847 5.4:+47387190 MS.gene010309:CDS
GAATTTAACTGATGAGATTA+AGG 0.298587 5.4:+47386983 MS.gene010309:CDS
CGACCCTGCCAAGGTTTGTT+GGG 0.299743 5.4:-47387857 None:intergenic
TCACTTGGTGCTTGGCTCAT+TGG 0.299947 5.4:-47387959 None:intergenic
TGCCAAGGATTGACTAGGTT+TGG 0.313165 5.4:-47387739 None:intergenic
ACCTCAAGCATGCGCCAATT+AGG 0.318991 5.4:-47387251 None:intergenic
TTGGCATATCTGTGCAATAT+TGG 0.331366 5.4:+47387992 MS.gene010309:CDS
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5.4:+47387119 MS.gene010309:CDS
ATTGGTATGCAGTCACAATT+AGG 0.352707 5.4:+47388010 MS.gene010309:CDS
CTACTTCCCACGAGGTCATC+TGG 0.355762 5.4:+47386425 MS.gene010309:CDS
TCGACCCTGCCAAGGTTTGT+TGG 0.360193 5.4:-47387858 None:intergenic
CCTCAAGCATGCGCCAATTA+GGG 0.367711 5.4:-47387250 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.375178 5.4:-47386387 None:intergenic
GATCTTTCCCAATCTTGCTT+TGG 0.377536 5.4:+47386731 MS.gene010309:CDS
AGATTTCCAACCAATCTTCA+TGG 0.383662 5.4:-47387117 None:intergenic
ATGACAATTTCTCAGCTTAA+GGG 0.392754 5.4:+47387191 MS.gene010309:CDS
AGACTCGTCCAAAGCAAGAT+TGG 0.400052 5.4:-47386739 None:intergenic
CTCGTGTGGAACCCAAAATA+TGG 0.402726 5.4:+47386349 MS.gene010309:CDS
CGCTTCATCTGTAGTGAACT+TGG 0.424216 5.4:-47386946 None:intergenic
AATTCCGACAGATATCTTTC+CGG 0.425831 5.4:-47386913 None:intergenic
CTTGGTCAGTTACGGGAGTC+AGG 0.426329 5.4:-47386581 None:intergenic
CGACCTTAAAAGCCGAGAAT+GGG 0.441124 5.4:+47386779 MS.gene010309:CDS
TGGAATCTTGACGTCGGGGC+CGG 0.443805 5.4:-47386382 None:intergenic
GTGTATTATCCGACAGCTAT+TGG 0.461034 5.4:+47387053 MS.gene010309:CDS
GACGGGAACTACAGGAGGAG+GGG 0.468379 5.4:-47386607 None:intergenic
TACGGGAGTCAGGAGCAGTT+TGG 0.469490 5.4:-47386571 None:intergenic
GTTGACGATGCCATGAAGAT+TGG 0.477758 5.4:+47387107 MS.gene010309:CDS
GACTCGTCCAAAGCAAGATT+GGG 0.479990 5.4:-47386738 None:intergenic
GATGAAAACAACAGAATCGT+TGG 0.490573 5.4:-47386880 None:intergenic
GAAGATTCATATTTCTTCAA+AGG 0.492904 5.4:-47387158 None:intergenic
ATGGTTCTTGTGGCAGCAGT+AGG 0.493323 5.4:-47386465 None:intergenic
TTATCATAGGTTAACTGGGA+TGG 0.493859 5.4:+47387697 MS.gene010309:intron
CCGACCTTAAAAGCCGAGAA+TGG 0.494002 5.4:+47386778 MS.gene010309:CDS
GACCCTGCCAAGGTTTGTTG+GGG 0.496074 5.4:-47387856 None:intergenic
GGCGGAGTTGACGGTGGAGG+AGG 0.496620 5.4:-47387797 None:intergenic
TGAGGCGGAGTTGACGGTGG+AGG 0.507469 5.4:-47387800 None:intergenic
ATCCAAACCTAGTCAATCCT+TGG 0.508187 5.4:+47387737 MS.gene010309:CDS
CAACCTGCCAAGGATTGACT+AGG 0.513736 5.4:-47387744 None:intergenic
AAGATATCTCACTTGGTGCT+TGG 0.515083 5.4:-47387967 None:intergenic
GAGACGATGCATGGAATGGA+TGG 0.515792 5.4:-47386504 None:intergenic
TCATCCTCTGCCACCTCGTG+TGG 0.517268 5.4:+47386335 MS.gene010309:CDS
TCCAACATCGTCTTCTTGGA+CGG 0.518377 5.4:-47386625 None:intergenic
AATCTCAGTAGATTACATAC+TGG 0.519388 5.4:+47386822 MS.gene010309:CDS
TCTTCTTGGACGGGAACTAC+AGG 0.522794 5.4:-47386615 None:intergenic
CCCTAATTGGCGCATGCTTG+AGG 0.522896 5.4:+47387250 MS.gene010309:CDS
TGGACGGGAACTACAGGAGG+AGG 0.523890 5.4:-47386609 None:intergenic
GGTTCCACACGAGGTGGCAG+AGG 0.527487 5.4:-47386339 None:intergenic
GGAAGAGACGATGCATGGAA+TGG 0.530637 5.4:-47386508 None:intergenic
TGGTTCTTGTGGCAGCAGTA+GGG 0.533159 5.4:-47386464 None:intergenic
TGACTAGGTTTGGATTCTGC+GGG 0.536979 5.4:-47387729 None:intergenic
GATGAACTCTCAGCAAGTCT+AGG 0.537540 5.4:-47387832 None:intergenic
GAGGAGGAAGTCGAAGGTGC+AGG 0.538875 5.4:-47387781 None:intergenic
AGATATCTGTCGGAATTCGC+AGG 0.543135 5.4:+47386919 MS.gene010309:CDS
ACTACAGATGAAGCGGATGA+AGG 0.543135 5.4:+47386954 MS.gene010309:CDS
CCCTGCCAAGGTTTGTTGGG+GGG 0.543970 5.4:-47387854 None:intergenic
ACGGGAACTACAGGAGGAGG+GGG 0.544584 5.4:-47386606 None:intergenic
TTTGGATGCAGTGGAATTGG+CGG 0.544683 5.4:+47387007 MS.gene010309:CDS
TTGTAAACTTCAACCTGCCA+AGG 0.544687 5.4:-47387754 None:intergenic
AAACCTAGTCAATCCTTGGC+AGG 0.546100 5.4:+47387741 MS.gene010309:CDS
ACGATGCCATGAAGATTGGT+TGG 0.558168 5.4:+47387111 MS.gene010309:CDS
GCCAATAAAGATATCTCACT+TGG 0.558384 5.4:-47387974 None:intergenic
ACCCTGCCAAGGTTTGTTGG+GGG 0.559662 5.4:-47387855 None:intergenic
TCAGTAGATTACATACTGGC+TGG 0.560711 5.4:+47386826 MS.gene010309:CDS
CCAACATCGTCTTCTTGGAC+GGG 0.562296 5.4:-47386624 None:intergenic
GGTTCTTGTGGCAGCAGTAG+GGG 0.568653 5.4:-47386463 None:intergenic
ATTATCCGACAGCTATTGGT+TGG 0.574808 5.4:+47387057 MS.gene010309:CDS
TTGACTAGGTTTGGATTCTG+CGG 0.581555 5.4:-47387730 None:intergenic
TGGACGGTAACGATCAAGAA+TGG 0.583957 5.4:-47386484 None:intergenic
CAAGTTCACTACAGATGAAG+CGG 0.584809 5.4:+47386947 MS.gene010309:CDS
AAAATATGGCAGATATGCGC+CGG 0.589425 5.4:+47386363 MS.gene010309:CDS
TGACAATTTCTCAGCTTAAG+GGG 0.599563 5.4:+47387192 MS.gene010309:CDS
TAATCTACTGAGATTTGCAT+AGG 0.604127 5.4:-47386814 None:intergenic
TCAACGGAAGAGACGATGCA+TGG 0.604313 5.4:-47386513 None:intergenic
CCCGTCCAAGAAGACGATGT+TGG 0.606646 5.4:+47386624 MS.gene010309:CDS
GCAAAGACCTCGTCGGTGTG+AGG 0.608714 5.4:-47386549 None:intergenic
CAAACCTTGGCAGGGTCGAG+TGG 0.615213 5.4:+47387862 MS.gene010309:CDS
GACCTTAAAAGCCGAGAATG+GGG 0.616896 5.4:+47386780 MS.gene010309:CDS
GGACGGGAACTACAGGAGGA+GGG 0.619395 5.4:-47386608 None:intergenic
CGATGCATGGAATGGATGGA+CGG 0.620709 5.4:-47386500 None:intergenic
ACAGATGAAGCGGATGAAGG+AGG 0.630115 5.4:+47386957 MS.gene010309:CDS
AATCTTTGGAATCTTGACGT+CGG 0.630358 5.4:-47386388 None:intergenic
ACGATCAAGAATGGTTCTTG+TGG 0.631537 5.4:-47386475 None:intergenic
TCTTGAGGCGGAGTTGACGG+TGG 0.632846 5.4:-47387803 None:intergenic
TCACGCCAACCAATAGCTGT+CGG 0.635734 5.4:-47387062 None:intergenic
TTGTGTGAGAGTCTTGACAT+AGG 0.637006 5.4:-47386661 None:intergenic
AGTACCACTCGACCCTGCCA+AGG 0.642496 5.4:-47387866 None:intergenic
TCTGCCGGAAAGATATCTGT+CGG 0.642648 5.4:+47386909 MS.gene010309:CDS
AGGATCAACAAAGAGATCAA+CGG 0.651533 5.4:-47386529 None:intergenic
ACCTTAAAAGCCGAGAATGG+GGG 0.652954 5.4:+47386781 MS.gene010309:CDS
TGTTGATCCTCACACCGACG+AGG 0.655379 5.4:+47386542 MS.gene010309:CDS
CAGTTTGGCAAAGACCTCGT+CGG 0.663811 5.4:-47386556 None:intergenic
AGGTGGCAGAGGATGAGACA+TGG 0.663852 5.4:-47386328 None:intergenic
TCTTTGGAATCTTGACGTCG+GGG 0.665271 5.4:-47386386 None:intergenic
ATCGAGTTAGAGCATTCGAT+AGG 0.670484 5.4:-47386711 None:intergenic
TCTTGGACGGGAACTACAGG+AGG 0.671507 5.4:-47386612 None:intergenic
CATGTTCCAGATGACCTCGT+GGG 0.672658 5.4:-47386431 None:intergenic
GCATGTTCCAGATGACCTCG+TGG 0.672744 5.4:-47386432 None:intergenic
ACGGTGGAGGAGGAAGTCGA+AGG 0.704506 5.4:-47387787 None:intergenic
AAAGTTTACTACTTCCCACG+AGG 0.761653 5.4:+47386417 MS.gene010309:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATTAAATAATCTTATCA+TGG - chr5.4:47387330-47387349 None:intergenic 15.0%
!!! GTTTTTTATCTTTTTATCAT+AGG + chr5.4:47387684-47387703 MS.gene010309:intron 15.0%
! AAAAGTTTCTACTTAGCATT+AGG - chr5.4:47387595-47387614 None:intergenic 25.0%
! GAAGATTCATATTTCTTCAA+AGG - chr5.4:47387161-47387180 None:intergenic 25.0%
! GAATTTAACTGATGAGATTA+AGG + chr5.4:47386983-47387002 MS.gene010309:CDS 25.0%
! GTACATATGCTTTCTTAATT+TGG + chr5.4:47387272-47387291 MS.gene010309:intron 25.0%
!! CTTTTTATCATAGGTTAACT+GGG + chr5.4:47387693-47387712 MS.gene010309:intron 25.0%
!! TCTTTTTATCATAGGTTAAC+TGG + chr5.4:47387692-47387711 MS.gene010309:intron 25.0%
!!! CTATTTTTAGTGAATATCAC+TGG - chr5.4:47387527-47387546 None:intergenic 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG - chr5.4:47386405-47386424 None:intergenic 25.0%
AATCTCAGTAGATTACATAC+TGG + chr5.4:47386822-47386841 MS.gene010309:CDS 30.0%
ACCAAGTGAGATATCTTTAT+TGG + chr5.4:47387973-47387992 MS.gene010309:CDS 30.0%
ATGACAATTTCTCAGCTTAA+GGG + chr5.4:47387191-47387210 MS.gene010309:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG + chr5.4:47387237-47387256 MS.gene010309:CDS 30.0%
TAATCTACTGAGATTTGCAT+AGG - chr5.4:47386817-47386836 None:intergenic 30.0%
! AACTGATGAGATTAAGGTTT+TGG + chr5.4:47386989-47387008 MS.gene010309:CDS 30.0%
!! AGGAGTATTTACAATGAGAA+AGG - chr5.4:47387575-47387594 None:intergenic 30.0%
AATTCCGACAGATATCTTTC+CGG - chr5.4:47386916-47386935 None:intergenic 35.0%
AGATTTCCAACCAATCTTCA+TGG - chr5.4:47387120-47387139 None:intergenic 35.0%
AGGATCAACAAAGAGATCAA+CGG - chr5.4:47386532-47386551 None:intergenic 35.0%
ATTGGTATGCAGTCACAATT+AGG + chr5.4:47388010-47388029 MS.gene010309:CDS 35.0%
CGTTGCTTCATAACCATAAT+AGG + chr5.4:47387935-47387954 MS.gene010309:intron 35.0%
CTATCAAGTGCATGAACAAT+GGG - chr5.4:47387446-47387465 None:intergenic 35.0%
GATGAAAACAACAGAATCGT+TGG - chr5.4:47386883-47386902 None:intergenic 35.0%
GATGACAATTTCTCAGCTTA+AGG + chr5.4:47387190-47387209 MS.gene010309:CDS 35.0%
GCCAATAAAGATATCTCACT+TGG - chr5.4:47387977-47387996 None:intergenic 35.0%
TACAATGAGAAAGGCTTAGA+AGG - chr5.4:47387566-47387585 None:intergenic 35.0%
TCTATCAAGTGCATGAACAA+TGG - chr5.4:47387447-47387466 None:intergenic 35.0%
TGACAATTTCTCAGCTTAAG+GGG + chr5.4:47387192-47387211 MS.gene010309:CDS 35.0%
TTATCATAGGTTAACTGGGA+TGG + chr5.4:47387697-47387716 MS.gene010309:intron 35.0%
TTGGCATATCTGTGCAATAT+TGG + chr5.4:47387992-47388011 MS.gene010309:CDS 35.0%
! AAGTCTAGGAGTTTTTCTTG+AGG - chr5.4:47387821-47387840 None:intergenic 35.0%
! ATGAAGATTGGTTGGAAATC+TGG + chr5.4:47387119-47387138 MS.gene010309:CDS 35.0%
! GTTTTCATCAAAAACTCTGC+CGG + chr5.4:47386894-47386913 MS.gene010309:CDS 35.0%
!! AATCTTTGGAATCTTGACGT+CGG - chr5.4:47386391-47386410 None:intergenic 35.0%
AAAATATGGCAGATATGCGC+CGG + chr5.4:47386363-47386382 MS.gene010309:CDS 40.0%
AAAGTTTACTACTTCCCACG+AGG + chr5.4:47386417-47386436 MS.gene010309:CDS 40.0%
ACGATCAAGAATGGTTCTTG+TGG - chr5.4:47386478-47386497 None:intergenic 40.0%
ATCCAAACCTAGTCAATCCT+TGG + chr5.4:47387737-47387756 MS.gene010309:CDS 40.0%
ATTATCCGACAGCTATTGGT+TGG + chr5.4:47387057-47387076 MS.gene010309:CDS 40.0%
CAAGTTCACTACAGATGAAG+CGG + chr5.4:47386947-47386966 MS.gene010309:CDS 40.0%
GATCTTTCCCAATCTTGCTT+TGG + chr5.4:47386731-47386750 MS.gene010309:CDS 40.0%
GTGTATTATCCGACAGCTAT+TGG + chr5.4:47387053-47387072 MS.gene010309:CDS 40.0%
TATATGTGTACCCCCATTCT+CGG - chr5.4:47386794-47386813 None:intergenic 40.0%
TCAGTAGATTACATACTGGC+TGG + chr5.4:47386826-47386845 MS.gene010309:CDS 40.0%
TGTATCCAACATCGTCTTCT+TGG - chr5.4:47386632-47386651 None:intergenic 40.0%
TTGACTAGGTTTGGATTCTG+CGG - chr5.4:47387733-47387752 None:intergenic 40.0%
TTGGCTCATTGGACCTATTA+TGG - chr5.4:47387951-47387970 None:intergenic 40.0%
TTGTAAACTTCAACCTGCCA+AGG - chr5.4:47387757-47387776 None:intergenic 40.0%
TTGTGTGAGAGTCTTGACAT+AGG - chr5.4:47386664-47386683 None:intergenic 40.0%
! ATCGAGTTAGAGCATTCGAT+AGG - chr5.4:47386714-47386733 None:intergenic 40.0%
! GCGCATATCTGCCATATTTT+GGG - chr5.4:47386363-47386382 None:intergenic 40.0%
! TCTAGGAGTTTTTCTTGAGG+CGG - chr5.4:47387818-47387837 None:intergenic 40.0%
!! AAGATATCTCACTTGGTGCT+TGG - chr5.4:47387970-47387989 None:intergenic 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG - chr5.4:47386390-47386409 None:intergenic 40.0%
!!! GATTAAGGTTTTGGATGCAG+TGG + chr5.4:47386998-47387017 MS.gene010309:CDS 40.0%
AAACCTAGTCAATCCTTGGC+AGG + chr5.4:47387741-47387760 MS.gene010309:CDS 45.0%
ACCTTAAAAGCCGAGAATGG+GGG + chr5.4:47386781-47386800 MS.gene010309:CDS 45.0%
ACGATGCCATGAAGATTGGT+TGG + chr5.4:47387111-47387130 MS.gene010309:CDS 45.0%
ACTACAGATGAAGCGGATGA+AGG + chr5.4:47386954-47386973 MS.gene010309:CDS 45.0%
AGACTCGTCCAAAGCAAGAT+TGG - chr5.4:47386742-47386761 None:intergenic 45.0%
AGATATCTGTCGGAATTCGC+AGG + chr5.4:47386919-47386938 MS.gene010309:CDS 45.0%
CCAAACCTTGGCAGTCTTAA+CGG + chr5.4:47387904-47387923 MS.gene010309:intron 45.0%
CGACCTTAAAAGCCGAGAAT+GGG + chr5.4:47386779-47386798 MS.gene010309:CDS 45.0%
CGCTTCATCTGTAGTGAACT+TGG - chr5.4:47386949-47386968 None:intergenic 45.0%
CTCGTGTGGAACCCAAAATA+TGG + chr5.4:47386349-47386368 MS.gene010309:CDS 45.0%
GACCTTAAAAGCCGAGAATG+GGG + chr5.4:47386780-47386799 MS.gene010309:CDS 45.0%
GACTCGTCCAAAGCAAGATT+GGG - chr5.4:47386741-47386760 None:intergenic 45.0%
GATGAACTCTCAGCAAGTCT+AGG - chr5.4:47387835-47387854 None:intergenic 45.0%
GTTGACGATGCCATGAAGAT+TGG + chr5.4:47387107-47387126 MS.gene010309:CDS 45.0%
TCCAACATCGTCTTCTTGGA+CGG - chr5.4:47386628-47386647 None:intergenic 45.0%
TCTGCCGGAAAGATATCTGT+CGG + chr5.4:47386909-47386928 MS.gene010309:CDS 45.0%
TGACTAGGTTTGGATTCTGC+GGG - chr5.4:47387732-47387751 None:intergenic 45.0%
TGCCAAGGATTGACTAGGTT+TGG - chr5.4:47387742-47387761 None:intergenic 45.0%
TGGACGGTAACGATCAAGAA+TGG - chr5.4:47386487-47386506 None:intergenic 45.0%
TTTGGATGCAGTGGAATTGG+CGG + chr5.4:47387007-47387026 MS.gene010309:CDS 45.0%
! GGCGCATATCTGCCATATTT+TGG - chr5.4:47386364-47386383 None:intergenic 45.0%
! TTTTCTTGAGGCGGAGTTGA+CGG - chr5.4:47387809-47387828 None:intergenic 45.0%
!! TCTTTGGAATCTTGACGTCG+GGG - chr5.4:47386389-47386408 None:intergenic 45.0%
!!! CATATTTTGGGTTCCACACG+AGG - chr5.4:47386351-47386370 None:intergenic 45.0%
!!! CCATTCTCGGCTTTTAAGGT+CGG - chr5.4:47386781-47386800 None:intergenic 45.0%
!!! GGTTTTGGATGCAGTGGAAT+TGG + chr5.4:47387004-47387023 MS.gene010309:CDS 45.0%
ACAGATGAAGCGGATGAAGG+AGG + chr5.4:47386957-47386976 MS.gene010309:CDS 50.0%
ACCTCAAGCATGCGCCAATT+AGG - chr5.4:47387254-47387273 None:intergenic 50.0%
AGGGGGTTCTTGGTCAGTTA+CGG - chr5.4:47386592-47386611 None:intergenic 50.0%
ATGGTTCTTGTGGCAGCAGT+AGG - chr5.4:47386468-47386487 None:intergenic 50.0%
CAACCTGCCAAGGATTGACT+AGG - chr5.4:47387747-47387766 None:intergenic 50.0%
CAGTTTGGCAAAGACCTCGT+CGG - chr5.4:47386559-47386578 None:intergenic 50.0%
CATGTTCCAGATGACCTCGT+GGG - chr5.4:47386434-47386453 None:intergenic 50.0%
CCAACATCGTCTTCTTGGAC+GGG - chr5.4:47386627-47386646 None:intergenic 50.0%
CCAAGGTTTGGCTATGTGTC+AGG - chr5.4:47387895-47387914 None:intergenic 50.0%
CCGACCTTAAAAGCCGAGAA+TGG + chr5.4:47386778-47386797 MS.gene010309:CDS 50.0%
CCGTTAAGACTGCCAAGGTT+TGG - chr5.4:47387907-47387926 None:intergenic 50.0%
CCTCAAGCATGCGCCAATTA+GGG - chr5.4:47387253-47387272 None:intergenic 50.0%
CCTGACACATAGCCAAACCT+TGG + chr5.4:47387892-47387911 MS.gene010309:intron 50.0%
CGATGCATGGAATGGATGGA+CGG - chr5.4:47386503-47386522 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG - chr5.4:47386507-47386526 None:intergenic 50.0%
GGAAGAGACGATGCATGGAA+TGG - chr5.4:47386511-47386530 None:intergenic 50.0%
TCAACGGAAGAGACGATGCA+TGG - chr5.4:47386516-47386535 None:intergenic 50.0%
TCACGCCAACCAATAGCTGT+CGG - chr5.4:47387065-47387084 None:intergenic 50.0%
TCTTCTTGGACGGGAACTAC+AGG - chr5.4:47386618-47386637 None:intergenic 50.0%
TGGTTCTTGTGGCAGCAGTA+GGG - chr5.4:47386467-47386486 None:intergenic 50.0%
TTCATCCCCCCAACAAACCT+TGG + chr5.4:47387849-47387868 MS.gene010309:CDS 50.0%
! ACCCCCATTCTCGGCTTTTA+AGG - chr5.4:47386785-47386804 None:intergenic 50.0%
! TGGTTGGCGTGATTTTGTCG+TGG + chr5.4:47387073-47387092 MS.gene010309:CDS 50.0%
!! TCACTTGGTGCTTGGCTCAT+TGG - chr5.4:47387962-47387981 None:intergenic 50.0%
!!! ATTTTGGGTTCCACACGAGG+TGG - chr5.4:47386348-47386367 None:intergenic 50.0%
CAGCACCGTTAAGACTGCCA+AGG - chr5.4:47387912-47387931 None:intergenic 55.0%
CCCGTCCAAGAAGACGATGT+TGG + chr5.4:47386624-47386643 MS.gene010309:CDS 55.0%
CCCTAATTGGCGCATGCTTG+AGG + chr5.4:47387250-47387269 MS.gene010309:CDS 55.0%
CTACTTCCCACGAGGTCATC+TGG + chr5.4:47386425-47386444 MS.gene010309:CDS 55.0%
CTTGGTCAGTTACGGGAGTC+AGG - chr5.4:47386584-47386603 None:intergenic 55.0%
GCATGTTCCAGATGACCTCG+TGG - chr5.4:47386435-47386454 None:intergenic 55.0%
GGGGGTTCTTGGTCAGTTAC+GGG - chr5.4:47386591-47386610 None:intergenic 55.0%
GGTTCTTGTGGCAGCAGTAG+GGG - chr5.4:47386466-47386485 None:intergenic 55.0%
TACGGGAGTCAGGAGCAGTT+TGG - chr5.4:47386574-47386593 None:intergenic 55.0%
TCGACCCTGCCAAGGTTTGT+TGG - chr5.4:47387861-47387880 None:intergenic 55.0%
TCTTGGACGGGAACTACAGG+AGG - chr5.4:47386615-47386634 None:intergenic 55.0%
TGTTGATCCTCACACCGACG+AGG + chr5.4:47386542-47386561 MS.gene010309:CDS 55.0%
! CGACCCTGCCAAGGTTTGTT+GGG - chr5.4:47387860-47387879 None:intergenic 55.0%
!! ACCCTGCCAAGGTTTGTTGG+GGG - chr5.4:47387858-47387877 None:intergenic 55.0%
!! GACCCTGCCAAGGTTTGTTG+GGG - chr5.4:47387859-47387878 None:intergenic 55.0%
ACGGGAACTACAGGAGGAGG+GGG - chr5.4:47386609-47386628 None:intergenic 60.0%
ACGGTGGAGGAGGAAGTCGA+AGG - chr5.4:47387790-47387809 None:intergenic 60.0%
AGTACCACTCGACCCTGCCA+AGG - chr5.4:47387869-47387888 None:intergenic 60.0%
CAAACCTTGGCAGGGTCGAG+TGG + chr5.4:47387862-47387881 MS.gene010309:CDS 60.0%
CCCCCCAACAAACCTTGGCA+GGG + chr5.4:47387854-47387873 MS.gene010309:CDS 60.0%
CTACAGGAGGAGGGGGTTCT+TGG - chr5.4:47386602-47386621 None:intergenic 60.0%
GACGGGAACTACAGGAGGAG+GGG - chr5.4:47386610-47386629 None:intergenic 60.0%
GCAAAGACCTCGTCGGTGTG+AGG - chr5.4:47386552-47386571 None:intergenic 60.0%
GGACGGGAACTACAGGAGGA+GGG - chr5.4:47386611-47386630 None:intergenic 60.0%
TCATCCTCTGCCACCTCGTG+TGG + chr5.4:47386335-47386354 MS.gene010309:CDS 60.0%
TCCCCCCAACAAACCTTGGC+AGG + chr5.4:47387853-47387872 MS.gene010309:CDS 60.0%
TCTTGAGGCGGAGTTGACGG+TGG - chr5.4:47387806-47387825 None:intergenic 60.0%
TGGAATCTTGACGTCGGGGC+CGG - chr5.4:47386385-47386404 None:intergenic 60.0%
TGGACGGGAACTACAGGAGG+AGG - chr5.4:47386612-47386631 None:intergenic 60.0%
! GAGGAGGAAGTCGAAGGTGC+AGG - chr5.4:47387784-47387803 None:intergenic 60.0%
!! CCCTGCCAAGGTTTGTTGGG+GGG - chr5.4:47387857-47387876 None:intergenic 60.0%
GGTTCCACACGAGGTGGCAG+AGG - chr5.4:47386342-47386361 None:intergenic 65.0%
TGAGGCGGAGTTGACGGTGG+AGG - chr5.4:47387803-47387822 None:intergenic 65.0%
GGCGGAGTTGACGGTGGAGG+AGG - chr5.4:47387800-47387819 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 47386330 47388042 47386330 ID=MS.gene010309
chr5.4 mRNA 47386330 47388042 47386330 ID=MS.gene010309.t1;Parent=MS.gene010309
chr5.4 exon 47386330 47387271 47386330 ID=MS.gene010309.t1.exon1;Parent=MS.gene010309.t1
chr5.4 CDS 47386330 47387271 47386330 ID=cds.MS.gene010309.t1;Parent=MS.gene010309.t1
chr5.4 exon 47387706 47387883 47387706 ID=MS.gene010309.t1.exon2;Parent=MS.gene010309.t1
chr5.4 CDS 47387706 47387883 47387706 ID=cds.MS.gene010309.t1;Parent=MS.gene010309.t1
chr5.4 exon 47387957 47388042 47387957 ID=MS.gene010309.t1.exon3;Parent=MS.gene010309.t1
chr5.4 CDS 47387957 47388042 47387957 ID=cds.MS.gene010309.t1;Parent=MS.gene010309.t1
Gene Sequence

>MS.gene010309

ATGTCTCATCCTCTGCCACCTCGTGTGGAACCCAAAATATGGCAGATATGCGCCGGCCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACGAGGTCATCTGGAACATGCATGTTCTTCCCCTACTGCTGCCACAAGAACCATTCTTGATCGTTACCGTCCATCCATTCCATGCATCGTCTCTTCCGTTGATCTCTTTGTTGATCCTCACACCGACGAGGTCTTTGCCAAACTGCTCCTGACTCCCGTAACTGACCAAGAACCCCCTCCTCCTGTAGTTCCCGTCCAAGAAGACGATGTTGGATACAATCTTGTTTCCTATGTCAAGACTCTCACACAATCTGATTGTGCAAGAGTATTATGCGTACCTATCGAATGCTCTAACTCGATCTTTCCCAATCTTGCTTTGGACGAGTCTCAGAGCATCACTGTCACCGACCTTAAAAGCCGAGAATGGGGGTACACATATACCTATGCAAATCTCAGTAGATTACATACTGGCTGGTTAAATTTTGTACGAGAGAAGAAACTCGTCGCCAACGATTCTGTTGTTTTCATCAAAAACTCTGCCGGAAAGATATCTGTCGGAATTCGCAGGAACACCAAGTTCACTACAGATGAAGCGGATGAAGGAGGCGAGAATTTAACTGATGAGATTAAGGTTTTGGATGCAGTGGAATTGGCGGAGAAGAACACAGCATTTGATGTTGTGTATTATCCGACAGCTATTGGTTGGCGTGATTTTGTCGTGGATGCTAAGACTGTTGACGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGAGTGAAACTTCCTTTGAAGAAATATGAATCTTCAAGTTCAAAGATGACAATTTCTCAGCTTAAGGGGACAATCTCTTTTGTCTTTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTGAGGTACATATGCTTTCTTAATTTGGTCTGATTATATTTCAATTGTTTATAGTTGAATCCATGATAAGATTATTTAATTGATATATATGAGTGTTGAAGTTAATACATGAAACTATAATTAGATGATACAGAAAAATGTAACAGAATCAGATTACACTTGAAATATTATTAATTCCCATTGTTCATGCACTTGATAGATATCTCATGACACTTAATGTTTACTTTATGTAATCAGATTGTTTTTATACGAAACTGCCAGTGATATTCACTAAAAATAGTTATGAAGTTTAAGAACCTTCTAAGCCTTTCTCATTGTAAATACTCCTAATGCTAAGTAGAAACTTTTGATGTCTAAATACATATATACTATATATATTTTATCAAATATTTCAAATTATTGATATTGATTCTTACTGTTTTTTATCTTTTTATCATAGGTTAACTGGGATGGACTTGATTTCCCGCAGAATCCAAACCTAGTCAATCCTTGGCAGGTTGAAGTTTACAACATACCTGCACCTTCGACTTCCTCCTCCACCGTCAACTCCGCCTCAAGAAAAACTCCTAGACTTGCTGAGAGTTCATCCCCCCAACAAACCTTGGCAGGGTCGAGTGGTACTTTGCCTGACACATAGCCAAACCTTGGCAGTCTTAACGGTGCTGACTCGTTGCTTCATAACCATAATAGGTCCAATGAGCCAAGCACCAAGTGAGATATCTTTATTGGCATATCTGTGCAATATTGGTATGCAGTCACAATTAGGAACTCAGTGA
Protein sequence

>MS.gene010309.t1

MSHPLPPRVEPKIWQICAGPDVKIPKIHSKVYYFPRGHLEHACSSPTAATRTILDRYRPSIPCIVSSVDLFVDPHTDEVFAKLLLTPVTDQEPPPPVVPVQEDDVGYNLVSYVKTLTQSDCARVLCVPIECSNSIFPNLALDESQSITVTDLKSREWGYTYTYANLSRLHTGWLNFVREKKLVANDSVVFIKNSAGKISVGIRRNTKFTTDEADEGGENLTDEIKVLDAVELAEKNTAFDVVYYPTAIGWRDFVVDAKTVDDAMKIGWKSGMRVKLPLKKYESSSSKMTISQLKGTISFVFNDSSNVPNWRMLEVNWDGLDFPQNPNLVNPWQVEVYNIPAPSTSSSTVNSASRKTPRLAESSSPQQTLAGSSGPMSQAPSEISLLAYLCNIGMQSQLGTQ