AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010311

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010311.t1 MTR_5g060770 72.727 209 17 2 4 212 50 218 1.23e-93 293
MS.gene010311.t1 MTR_5g060780 70.892 213 58 3 4 212 102 314 1.94e-90 286
MS.gene010311.t1 MTR_5g060780 70.968 93 21 2 539 631 312 398 2.67e-29 120
MS.gene010311.t1 MTR_5g460920 70.892 213 58 3 4 212 102 314 8.98e-90 284
MS.gene010311.t1 MTR_5g460920 70.968 93 21 2 539 631 312 398 3.10e-29 120
MS.gene010311.t1 MTR_5g061890 69.484 213 61 3 4 212 102 314 6.98e-88 280
MS.gene010311.t1 MTR_5g061890 72.826 92 19 2 539 630 312 397 6.12e-31 125
MS.gene010311.t1 MTR_5g060630 58.636 220 79 5 4 212 131 349 4.97e-73 244
MS.gene010311.t1 MTR_5g460740 58.636 220 79 5 4 212 131 349 4.97e-73 244
MS.gene010311.t1 MTR_5g061220 53.878 245 71 8 4 212 108 346 8.35e-69 233
MS.gene010311.t1 MTR_5g460910 59.809 209 22 3 4 212 67 213 9.86e-68 224
MS.gene010311.t1 MTR_1g058210 52.814 231 92 8 3 221 101 326 2.03e-57 201
MS.gene010311.t1 MTR_2g024430 47.945 219 86 9 8 212 116 320 2.14e-48 177
MS.gene010311.t1 MTR_5g040880 45.249 221 100 8 4 214 103 312 2.52e-47 174
MS.gene010311.t1 MTR_5g040740 47.768 224 87 10 7 214 106 315 3.78e-47 171
MS.gene010311.t1 MTR_2g006270 49.083 218 90 10 6 212 108 315 4.45e-47 172
MS.gene010311.t1 MTR_2g006380 43.961 207 94 9 13 212 126 317 3.98e-40 153
MS.gene010311.t1 MTR_5g082140 43.379 219 99 7 8 212 108 315 1.30e-39 151
MS.gene010311.t1 MTR_3g073420 36.641 262 104 10 4 212 107 359 8.65e-33 134
MS.gene010311.t1 MTR_5g062960 63.333 90 31 2 126 214 1 89 1.53e-29 116
MS.gene010311.t1 MTR_1g064430 31.783 258 113 10 12 212 127 378 3.70e-24 107
MS.gene010311.t1 MTR_1g064430 31.783 258 113 10 12 212 140 391 4.47e-24 107
MS.gene010311.t1 MTR_4g058930 32.171 258 113 9 13 212 108 361 1.08e-23 106
MS.gene010311.t1 MTR_4g058930 32.171 258 113 9 13 212 108 361 1.10e-23 106
MS.gene010311.t1 MTR_1g094960 32.353 238 101 8 12 198 116 344 4.79e-23 104
MS.gene010311.t1 MTR_2g094570 30.952 252 118 9 13 212 107 354 5.14e-23 104
MS.gene010311.t1 MTR_2g094570 30.952 252 118 9 13 212 107 354 5.27e-23 104
MS.gene010311.t1 MTR_2g094570 30.952 252 118 9 13 212 163 410 6.83e-23 104
MS.gene010311.t1 MTR_7g101275 31.628 215 127 8 13 212 91 300 2.26e-22 100
MS.gene010311.t1 MTR_7g101280 28.244 262 120 10 13 212 130 385 1.02e-18 90.9
MS.gene010311.t1 MTR_0041s0110 28.244 262 120 10 13 212 130 385 1.02e-18 90.9
MS.gene010311.t1 MTR_5g062970 45.794 107 48 3 4 100 128 234 5.94e-18 84.0
MS.gene010311.t1 MTR_1g111260 49.533 107 43 3 323 428 81 177 2.78e-17 80.9
MS.gene010311.t1 MTR_2g018690 32.287 223 108 11 14 209 127 333 9.45e-17 84.7
MS.gene010311.t1 MTR_0041s0120 29.646 226 129 9 13 212 91 312 1.81e-16 82.4
MS.gene010311.t1 MTR_2g014770 28.108 185 120 5 14 186 158 341 3.03e-16 82.8
MS.gene010311.t1 MTR_2g014770 28.108 185 120 5 14 186 158 341 3.55e-16 82.4
MS.gene010311.t1 MTR_4g124900 30.000 180 115 4 14 182 126 305 5.65e-16 82.4
MS.gene010311.t1 MTR_4g124900 30.000 180 115 4 14 182 126 305 6.00e-16 82.4
MS.gene010311.t1 MTR_4g088210 27.027 185 122 5 14 186 148 331 9.20e-16 81.3
MS.gene010311.t1 MTR_8g079492 31.222 221 113 10 14 209 126 332 9.54e-16 81.6
MS.gene010311.t1 MTR_4g088210 27.027 185 122 5 14 186 148 331 9.73e-16 81.3
MS.gene010311.t1 MTR_8g079492 31.222 221 113 10 14 209 44 250 9.92e-16 81.6
MS.gene010311.t1 MTR_8g079492 31.222 221 113 10 14 209 126 332 1.01e-15 81.6
MS.gene010311.t1 MTR_2g043250 29.592 196 123 6 14 198 126 317 1.36e-15 81.3
MS.gene010311.t1 MTR_4g060460 31.217 189 111 8 14 187 166 350 2.40e-15 80.1
MS.gene010311.t1 MTR_4g060460 31.217 189 111 8 14 187 166 350 2.52e-15 80.1
MS.gene010311.t1 MTR_3g064050 30.918 207 107 8 14 198 130 322 2.62e-15 80.1
MS.gene010311.t1 MTR_2g005240 29.680 219 130 7 13 214 124 335 3.37e-15 79.7
MS.gene010311.t1 MTR_5g076270 30.918 207 107 8 14 198 128 320 3.56e-15 79.7
MS.gene010311.t1 MTR_2g093740 29.870 231 135 9 4 211 150 376 3.83e-15 79.3
MS.gene010311.t1 MTR_2g093740 29.741 232 134 9 4 211 20 246 4.23e-15 79.3
MS.gene010311.t1 MTR_2g093740 29.870 231 135 9 4 211 20 246 4.74e-15 79.0
MS.gene010311.t1 MTR_8g100050 27.397 219 135 7 13 214 161 372 5.56e-15 79.0
MS.gene010311.t1 MTR_2g093740 29.741 232 134 9 4 211 150 376 5.79e-15 79.0
MS.gene010311.t1 MTR_2g093740 29.870 231 135 9 4 211 150 376 6.20e-15 79.0
MS.gene010311.t1 MTR_8g100050 27.397 219 135 7 13 214 161 372 7.37e-15 78.6
MS.gene010311.t1 MTR_7g062540 28.512 242 145 9 13 233 115 349 3.09e-14 76.6
MS.gene010311.t1 MTR_4g021580 28.333 240 150 8 13 234 120 355 3.97e-14 76.3
MS.gene010311.t1 MTR_4g021580 28.333 240 150 8 13 234 120 355 5.78e-14 75.5
MS.gene010311.t1 MTR_1g024025 30.890 191 108 9 14 186 146 330 4.24e-13 73.2
MS.gene010311.t1 MTR_8g027440 27.984 243 144 10 13 233 130 363 2.64e-12 70.5
MS.gene010311.t1 MTR_4g091820 40.000 145 64 6 338 474 822 951 3.04e-12 70.5
MS.gene010311.t1 MTR_2g093740 28.279 244 135 10 4 211 150 389 3.65e-12 70.1
MS.gene010311.t1 MTR_2g093740 28.163 245 134 10 4 211 20 259 3.98e-12 69.7
MS.gene010311.t1 MTR_2g093740 28.163 245 134 10 4 211 20 259 4.55e-12 69.7
MS.gene010311.t1 MTR_2g093740 28.163 245 134 10 4 211 150 389 4.66e-12 69.7
MS.gene010311.t1 MTR_8g101360 35.849 106 58 3 14 109 135 240 5.05e-12 69.7
MS.gene010311.t1 MTR_2g093740 28.279 244 135 10 4 211 150 389 5.33e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010311.t1 AT1G77850 31.513 238 118 9 3 199 108 341 1.65e-27 118
MS.gene010311.t1 AT1G77850 31.513 238 118 9 3 199 108 341 1.65e-27 117
MS.gene010311.t1 AT4G30080 33.865 251 108 11 13 212 119 362 2.38e-25 111
MS.gene010311.t1 AT2G28350 30.556 252 120 10 12 212 113 360 6.10e-21 97.8
MS.gene010311.t1 AT2G28350 30.556 252 120 10 12 212 113 360 7.35e-21 97.4
MS.gene010311.t1 AT2G33860 31.053 190 110 7 14 187 2 186 1.24e-17 86.3
MS.gene010311.t1 AT2G33860 31.053 190 110 7 14 187 159 343 2.45e-17 86.3
MS.gene010311.t1 AT1G30330 31.884 207 105 8 14 198 127 319 2.54e-16 83.6
MS.gene010311.t1 AT1G30330 31.884 207 105 8 14 198 129 321 2.68e-16 83.6
MS.gene010311.t1 AT1G30330 31.884 207 105 8 14 198 129 321 2.68e-16 83.6
MS.gene010311.t1 AT2G46530 29.796 245 142 10 13 236 36 271 4.57e-16 82.0
MS.gene010311.t1 AT5G37020 30.317 221 115 10 14 209 126 332 5.16e-16 82.4
MS.gene010311.t1 AT5G37020 30.317 221 115 10 14 209 126 332 6.24e-16 82.0
MS.gene010311.t1 AT2G46530 29.796 245 142 10 13 236 123 358 6.46e-16 81.6
MS.gene010311.t1 AT2G46530 29.796 245 142 10 13 236 144 379 6.49e-16 81.6
MS.gene010311.t1 AT5G37020 30.317 221 115 10 14 209 31 237 7.69e-16 81.6
MS.gene010311.t1 AT1G34390 28.112 249 138 9 13 236 125 357 7.26e-15 78.6
MS.gene010311.t1 AT1G59750 29.545 220 129 9 13 214 123 334 1.09e-14 78.2
MS.gene010311.t1 AT1G59750 29.545 220 129 9 13 214 123 334 1.09e-14 78.2
MS.gene010311.t1 AT1G59750 29.545 220 129 9 13 214 123 334 1.18e-14 77.8
MS.gene010311.t1 AT1G59750 29.545 220 129 9 13 214 123 334 1.19e-14 77.8
MS.gene010311.t1 AT5G20730 31.111 180 112 5 14 181 127 306 1.50e-14 78.2
MS.gene010311.t1 AT5G20730 31.111 180 112 5 14 181 127 306 1.58e-14 77.8
MS.gene010311.t1 AT5G20730 31.667 180 110 6 14 181 127 305 2.08e-14 77.4
MS.gene010311.t1 AT1G34410 28.862 246 142 11 13 236 125 359 2.37e-14 76.6
MS.gene010311.t1 AT3G61830 27.572 243 146 10 13 234 76 309 2.40e-14 76.6
MS.gene010311.t1 AT3G61830 27.572 243 146 10 13 234 127 360 3.02e-14 76.6
MS.gene010311.t1 AT1G35520 28.862 246 142 11 13 236 125 359 3.97e-14 76.3
MS.gene010311.t1 AT5G62000 27.660 188 119 5 13 186 163 347 6.35e-14 75.9
MS.gene010311.t1 AT5G62000 27.660 188 119 5 13 186 163 347 6.35e-14 75.9
MS.gene010311.t1 AT5G62000 27.660 188 119 5 13 186 163 347 6.35e-14 75.9
MS.gene010311.t1 AT5G62000 27.660 188 119 5 13 186 163 347 6.35e-14 75.9
MS.gene010311.t1 AT1G35240 29.268 246 141 11 13 236 125 359 6.86e-14 75.5
MS.gene010311.t1 AT5G62000 27.660 188 119 5 13 186 163 347 6.96e-14 75.9
MS.gene010311.t1 AT1G34310 29.680 219 127 9 13 214 125 333 1.18e-13 74.7
MS.gene010311.t1 AT1G19220 28.333 180 116 6 14 181 126 304 5.02e-13 73.2
MS.gene010311.t1 AT5G60450 28.837 215 128 10 14 211 153 359 6.95e-13 72.4
MS.gene010311.t1 AT5G60450 28.837 215 128 10 14 211 177 383 7.78e-13 72.4
MS.gene010311.t1 AT1G35540 27.064 218 134 8 13 214 125 333 1.39e-12 71.2
MS.gene010311.t1 AT4G23980 26.613 248 154 9 13 242 115 352 2.54e-12 70.5
MS.gene010311.t1 AT4G23980 26.613 248 154 9 13 242 115 352 2.57e-12 70.5
MS.gene010311.t1 AT1G19850 37.624 101 53 3 14 104 147 247 3.07e-12 70.5
MS.gene010311.t1 AT1G19850 37.624 101 53 3 14 104 158 258 3.11e-12 70.5

Find 135 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACCATCTTGAAGAAATTT+TGG 0.168199 5.4:-47355749 MS.gene010311:CDS
TGAGGATTCTGATTCTAATT+TGG 0.189632 5.4:-47355712 MS.gene010311:CDS
CCACTTGGTGCTTGGTTCAT+TGG 0.201903 5.4:+47354841 None:intergenic
CATGAAAACAACAGAATCTT+TGG 0.216238 5.4:+47357291 None:intergenic
TCGCTTTATCATAGGTTAAC+TGG 0.238481 5.4:-47355117 MS.gene010311:intron
CATTGAATGTGGAATTTGTT+GGG 0.253252 5.4:+47354952 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.254844 5.4:-47356940 MS.gene010311:CDS
TTCTGATTCTAATTTGGATT+TGG 0.272826 5.4:-47355706 MS.gene010311:CDS
ATGAAGATTGGTTGGGAATC+TGG 0.311617 5.4:-47357055 MS.gene010311:CDS
TAAATTGCGAACCAGGATTA+AGG 0.323232 5.4:+47355353 None:intergenic
CTTTCACCCGCTTCAGCTGC+AGG 0.324014 5.4:+47357217 None:intergenic
ACCTCAAGAATGCGCCAATT+AGG 0.328543 5.4:+47356926 None:intergenic
GACTTCTCCAAGTCAAGCTT+TGG 0.342525 5.4:+47357427 None:intergenic
TTGGCATATCTGTGCAAAAT+TGG 0.343048 5.4:-47354808 MS.gene010311:CDS
CTCTCATAACATAGTTGTTC+AGG 0.345225 5.4:-47356249 MS.gene010311:CDS
GGTGTTGCATTTACCATTGC+TGG 0.348644 5.4:+47356131 None:intergenic
AGTTACCACGGGCTATCTTT+TGG 0.356248 5.4:+47356432 None:intergenic
CCTCAAGAATGCGCCAATTA+GGG 0.362876 5.4:+47356927 None:intergenic
TGTAATATGTCAAAAGAGTT+TGG 0.379094 5.4:+47356110 None:intergenic
GGTCTGCACCAGTCACAATT+AGG 0.381344 5.4:-47354787 MS.gene010311:CDS
CCATTGAATGTGGAATTTGT+TGG 0.382992 5.4:+47354951 None:intergenic
CATAAATACCCGCTCTAAGA+AGG 0.387184 5.4:-47355586 MS.gene010311:CDS
CTTGAAATAAGAATGTTTAA+CGG 0.403523 5.4:-47358138 None:intergenic
AATCTCACATATGGAGGCCT+TGG 0.406321 5.4:-47356075 MS.gene010311:CDS
TGTTGTGCAGTCACCAAACT+TGG 0.406376 5.4:+47355956 None:intergenic
TGTTGCTAAGGGATAGCCTT+AGG 0.408520 5.4:-47355410 MS.gene010311:CDS
ACGGATGCTAGCTCCAAGTT+TGG 0.411673 5.4:-47355969 MS.gene010311:CDS
AGATTCCCAACCAATCTTCA+TGG 0.412679 5.4:+47357057 None:intergenic
CGCTTTATCATAGGTTAACT+GGG 0.412912 5.4:-47355116 MS.gene010311:intron
GGTCTCAACACTGTGCTAAC+AGG 0.414237 5.4:+47355807 None:intergenic
ATTGTTCCCTGCTAAGTTGA+TGG 0.414664 5.4:+47355677 None:intergenic
AGTTGCTTCTGTTACAGAAA+AGG 0.424617 5.4:-47356285 MS.gene010311:CDS
AACTTCCCTGCAGCTGAAGC+GGG 0.439382 5.4:-47357223 MS.gene010311:CDS
AACAAGATTGTTTCCATCGT+CGG 0.447748 5.4:+47357528 None:intergenic
ATTATCCAACAGCTAGGAAT+TGG 0.450128 5.4:-47357117 MS.gene010311:CDS
GACAACCCCATCAACTTAGC+AGG 0.451331 5.4:-47355684 MS.gene010311:CDS
ACCCCAAAATTTCTTCAAGA+TGG 0.452437 5.4:+47355746 None:intergenic
AAGCATAATGTTGGCAAGCA+TGG 0.453185 5.4:-47356362 MS.gene010311:CDS
CGCTTCAGCTGCAGGGAAGT+TGG 0.459331 5.4:+47357225 None:intergenic
TGTTGCTAAAGGATAGCCTT+AGG 0.460863 5.4:-47355449 MS.gene010311:intron
TTGTTCCCTGCTAAGTTGAT+GGG 0.471487 5.4:+47355678 None:intergenic
CCGGAGTTCCTAATTGTGAC+TGG 0.476223 5.4:+47354779 None:intergenic
GATCTTTCCAAAGCTTGACT+TGG 0.477680 5.4:-47357434 MS.gene010311:CDS
ACCAAGTGGGGTATCTTCAT+TGG 0.478099 5.4:-47354827 MS.gene010311:CDS
GCAGCTGAAGCGGGTGAAAG+AGG 0.481113 5.4:-47357214 MS.gene010311:CDS
CAACTTCCCTGCAGCTGAAG+CGG 0.481220 5.4:-47357224 MS.gene010311:CDS
CTGAAGCGGGTGAAAGAGGC+TGG 0.483732 5.4:-47357210 MS.gene010311:CDS
AAGATACCCCACTTGGTGCT+TGG 0.484011 5.4:+47354833 None:intergenic
TAACTCTACTATTCAACATA+AGG 0.487096 5.4:-47356156 MS.gene010311:CDS
CCAACAAATTCCACATTCAA+TGG 0.487436 5.4:-47354951 MS.gene010311:CDS
CTTAGCAACAAAAGAAATGA+AGG 0.488051 5.4:+47355423 None:intergenic
AAAGGTTCAGGCCTTAATCC+TGG 0.488285 5.4:-47355364 MS.gene010311:intron
GTAGTGATTGGTTGTGGCAT+GGG 0.488467 5.4:+47355861 None:intergenic
GACTCCACTGACTTAGCATC+TGG 0.491860 5.4:-47356029 MS.gene010311:CDS
CTCACCTCAACAAAAGCTCC+TGG 0.493507 5.4:-47354995 MS.gene010311:CDS
CAAATTCCACATTCAATGGC+AGG 0.495396 5.4:-47354947 MS.gene010311:CDS
GTAGATGATGCCATGAAGAT+TGG 0.497274 5.4:-47357067 MS.gene010311:CDS
CCCTAATTGGCGCATTCTTG+AGG 0.497342 5.4:-47356927 MS.gene010311:intron
CATAGGATCCAAGCTTGAAA+AGG 0.506603 5.4:+47355917 None:intergenic
TCACACCAATTCCTAGCTGT+TGG 0.507314 5.4:+47357112 None:intergenic
ATACAAACATAGTCAATCCT+TGG 0.507379 5.4:-47355072 MS.gene010311:CDS
TTATCATAGGTTAACTGGGA+TGG 0.511289 5.4:-47355112 MS.gene010311:intron
AAATATTAGTTGGAATTCGC+AGG 0.515553 5.4:-47357252 MS.gene010311:CDS
AAAGACATATTATCAGCCTA+AGG 0.521760 5.4:-47356987 MS.gene010311:CDS
AGCATAATGTTGGCAAGCAT+GGG 0.524629 5.4:-47356361 MS.gene010311:CDS
GCAGCGGATGGAGTAAGAAC+CGG 0.524983 5.4:+47355786 None:intergenic
GCTATCAACAAGCATAATGT+TGG 0.527690 5.4:-47356371 MS.gene010311:CDS
AGTCGAAGGTGCAGGTATGT+TGG 0.528919 5.4:+47355036 None:intergenic
AGATTTACCCTTCTTAGAGC+GGG 0.530837 5.4:+47355578 None:intergenic
CGGGTATTTATGCCTTCAGA+AGG 0.536410 5.4:+47355597 None:intergenic
CAGTCACAATTAGGAACTCC+GGG 0.538501 5.4:-47354778 MS.gene010311:CDS
CTCGACCCTGCCATTGAATG+TGG 0.538653 5.4:+47354941 None:intergenic
TTTGAATTCAGTGGAATTAG+CGG 0.539615 5.4:-47357167 MS.gene010311:CDS
ATAGTTGTTCAGGCAGATCA+AGG 0.539702 5.4:-47356239 MS.gene010311:CDS
TGGTGACTGCACAACAAACT+TGG 0.542194 5.4:-47355949 MS.gene010311:CDS
AGAGAAGTGTAAGTAGTGAT+TGG 0.543922 5.4:+47355849 None:intergenic
ACATATGGAGGCCTTGGCTG+AGG 0.544156 5.4:-47356069 MS.gene010311:CDS
AAACATAGTCAATCCTTGGC+AGG 0.544769 5.4:-47355068 MS.gene010311:CDS
GCCTTGGCTGAGGTGATGAA+TGG 0.546511 5.4:-47356059 MS.gene010311:CDS
CCAGTCACAATTAGGAACTC+CGG 0.548542 5.4:-47354779 MS.gene010311:CDS
AAGGACGAAGTCGAAGGTGC+AGG 0.551572 5.4:+47355028 None:intergenic
ATTGAATGTGGAATTTGTTG+GGG 0.553077 5.4:+47354953 None:intergenic
ATGATGCCATGAAGATTGGT+TGG 0.554453 5.4:-47357063 MS.gene010311:CDS
ACAGTAGAGATTGTCCCCTT+AGG 0.555913 5.4:+47356971 None:intergenic
AAGCTTGCTGCCCCTTCTGA+AGG 0.557940 5.4:-47355609 MS.gene010311:CDS
GATAAACTCTCAGCAAGTCC+AGG 0.559515 5.4:+47354977 None:intergenic
TGTTAGCACAGTGTTGAGAC+CGG 0.564165 5.4:-47355805 MS.gene010311:CDS
TTGGAAACTTCAACCTGCCA+AGG 0.564377 5.4:+47355055 None:intergenic
TTGGGGTGATTATCTTGATG+AGG 0.564990 5.4:-47355730 MS.gene010311:CDS
AGTAGTGATTGGTTGTGGCA+TGG 0.567366 5.4:+47355860 None:intergenic
ACTATAATACTATGAGACAT+AGG 0.569110 5.4:-47356184 MS.gene010311:CDS
TGATGCCATGAAGATTGGTT+GGG 0.569149 5.4:-47357062 MS.gene010311:CDS
TTTCACCCGCTTCAGCTGCA+GGG 0.570879 5.4:+47357218 None:intergenic
AAGACATATTATCAGCCTAA+GGG 0.574496 5.4:-47356986 MS.gene010311:CDS
GGTATTTATGCCTTCAGAAG+GGG 0.575733 5.4:+47355599 None:intergenic
AGATGCTACTAAGTTACCAC+GGG 0.578730 5.4:+47356421 None:intergenic
GGGTATTTATGCCTTCAGAA+GGG 0.579372 5.4:+47355598 None:intergenic
GGAATTAGCGGACAAGAACA+TGG 0.580535 5.4:-47357155 MS.gene010311:CDS
TGATTTCGTGATGGATGCTA+AGG 0.581643 5.4:-47357092 MS.gene010311:CDS
GTGTAAGTAGTGATTGGTTG+TGG 0.582371 5.4:+47355855 None:intergenic
ACCATTCATCACCTCAGCCA+AGG 0.584271 5.4:+47356058 None:intergenic
GCAAAATCATACTGCAGGAG+TGG 0.585696 5.4:+47355554 None:intergenic
TCAACATAAGGTACCAGCAA+TGG 0.586437 5.4:-47356144 MS.gene010311:CDS
ATAAATACCCGCTCTAAGAA+GGG 0.587360 5.4:-47355585 MS.gene010311:CDS
AAAGAAATGAAGGAAGCCTA+AGG 0.588624 5.4:+47355394 None:intergenic
AAAGAAATGAAGGAAGCCTA+AGG 0.588624 5.4:+47355433 None:intergenic
TGTTCCCTGCTAAGTTGATG+GGG 0.592957 5.4:+47355679 None:intergenic
TTGTGTATTATCCAACAGCT+AGG 0.593766 5.4:-47357123 MS.gene010311:CDS
TAACTTAGTAGCATCTAAAG+AGG 0.594459 5.4:-47356414 MS.gene010311:CDS
CATTCAATGGCAGGGTCGAG+TGG 0.596117 5.4:-47354938 MS.gene010311:intron
CTGGAATTTAACTGAGAGTG+CGG 0.599476 5.4:-47357191 MS.gene010311:CDS
TCAGTAGACTACGTACTGGC+TGG 0.603214 5.4:-47357345 MS.gene010311:CDS
TGAGGTGAGGTTGACGGTGA+AGG 0.605499 5.4:+47355009 None:intergenic
ATACAAAACAGAAGAAGCAA+AGG 0.607779 5.4:-47355635 MS.gene010311:CDS
TTGAATGTGGAATTTGTTGG+GGG 0.613056 5.4:+47354954 None:intergenic
ATCAAGTCAGCGCATTCATG+AGG 0.615421 5.4:+47357454 None:intergenic
AAACTCAGTAGACTACGTAC+TGG 0.617424 5.4:-47357349 MS.gene010311:CDS
TGAAGCTGCTATTCACACTA+CGG 0.617473 5.4:-47355988 MS.gene010311:CDS
GCCAATGAAGATACCCCACT+TGG 0.618712 5.4:+47354826 None:intergenic
CAATGAACCAAGCACCAAGT+GGG 0.619421 5.4:-47354840 MS.gene010311:CDS
AAATTCCACATTCAATGGCA+GGG 0.627736 5.4:-47354946 MS.gene010311:CDS
AGACATATTATCAGCCTAAG+GGG 0.632162 5.4:-47356985 MS.gene010311:CDS
TCCTGGCCAGAAGCCGACGA+TGG 0.632989 5.4:-47357541 MS.gene010311:intron
TAGATTTACCCTTCTTAGAG+CGG 0.637382 5.4:+47355577 None:intergenic
ATCTGAAAAGAATAGCATAG+TGG 0.637957 5.4:-47355892 MS.gene010311:CDS
TAGATGCTACTAAGTTACCA+CGG 0.640398 5.4:+47356420 None:intergenic
GAATTGGTGTGATTTCGTGA+TGG 0.652593 5.4:-47357101 MS.gene010311:CDS
ACAACCCCATCAACTTAGCA+GGG 0.660843 5.4:-47355683 MS.gene010311:CDS
CCAATGAACCAAGCACCAAG+TGG 0.666119 5.4:-47354841 MS.gene010311:CDS
TACACCAGATGCTAAGTCAG+TGG 0.671071 5.4:+47356025 None:intergenic
TGAATGTGGAATTTGTTGGG+GGG 0.674958 5.4:+47354955 None:intergenic
AATGAACCAAGCACCAAGTG+GGG 0.678816 5.4:-47354839 MS.gene010311:CDS
CAGTGCCAAAAGATAGCCCG+TGG 0.684488 5.4:-47356437 MS.gene010311:CDS
ACGGTGAAGGACGAAGTCGA+AGG 0.716732 5.4:+47355022 None:intergenic
TAGTCTACTGAGTTTCACGT+AGG 0.730433 5.4:+47357357 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTGACTGAAAAAAATA+AGG - chr5.4:47356555-47356574 MS.gene010311:intron 15.0%
!! TACAATTATTGCAAAATAAT+GGG + chr5.4:47356411-47356430 None:intergenic 15.0%
!! TCAAACAAATCATAAAATTA+AGG + chr5.4:47356054-47356073 None:intergenic 15.0%
!! TGAAATAGATATACAAAATT+TGG - chr5.4:47356128-47356147 MS.gene010311:CDS 15.0%
!! TTACAATTATTGCAAAATAA+TGG + chr5.4:47356412-47356431 None:intergenic 15.0%
!!! AGAATCATATAATATTTTTC+AGG - chr5.4:47354813-47354832 MS.gene010311:CDS 15.0%
!!! GTATATATTTTAACATAAGA+TGG + chr5.4:47354925-47354944 None:intergenic 15.0%
!! ATATAATAATCGGTATATTG+TGG + chr5.4:47355166-47355185 None:intergenic 20.0%
!! ATTTGTATTTAAACATCCAA+AGG + chr5.4:47357696-47357715 None:intergenic 20.0%
!! TGATAAAGCGATAAAAAAAA+TGG + chr5.4:47357765-47357784 None:intergenic 20.0%
!!! AATTCCAACTAATATTTTTC+CGG + chr5.4:47355636-47355655 None:intergenic 20.0%
!!! ATATTTTAACATAAGATGGA+AGG + chr5.4:47354921-47354940 None:intergenic 20.0%
!!! CAAAATTTTCGAATTACATT+TGG + chr5.4:47356189-47356208 None:intergenic 20.0%
!!! TATTTTAACATAAGATGGAA+GGG + chr5.4:47354920-47354939 None:intergenic 20.0%
!!! TTCATTTCTTTTGTGTAAAA+AGG - chr5.4:47357509-47357528 MS.gene010311:CDS 20.0%
!!! TTTGTATTTTGCAAGATTTT+AGG + chr5.4:47357806-47357825 None:intergenic 20.0%
!!! TTTTGTTTTTTTGTTGCTAA+AGG - chr5.4:47357431-47357450 MS.gene010311:CDS 20.0%
!!! TTTTTACACAAAAGAAATGA+AGG + chr5.4:47357510-47357529 None:intergenic 20.0%
!!! TTTTTTTATCGCTTTATCAT+AGG - chr5.4:47357766-47357785 MS.gene010311:intron 20.0%
! ACAAAAAAACAAAAACTGAG+AGG + chr5.4:47357425-47357444 None:intergenic 25.0%
! ACTATAATACTATGAGACAT+AGG - chr5.4:47356707-47356726 MS.gene010311:intron 25.0%
! CAATGCTTTATAACCATAAT+AGG - chr5.4:47358026-47358045 MS.gene010311:intron 25.0%
! CGTGGTTGAAATATAATAAT+CGG + chr5.4:47355176-47355195 None:intergenic 25.0%
! TAACTCTACTATTCAACATA+AGG - chr5.4:47356735-47356754 MS.gene010311:intron 25.0%
! TATCAATTGATAAGAACCTT+TGG - chr5.4:47357677-47357696 MS.gene010311:intron 25.0%
! TGCAATAATTGTAAGTCTAT+TGG - chr5.4:47356418-47356437 MS.gene010311:CDS 25.0%
! TGTAATATGTCAAAAGAGTT+TGG + chr5.4:47356784-47356803 None:intergenic 25.0%
! TTCTGATTCTAATTTGGATT+TGG - chr5.4:47357185-47357204 MS.gene010311:CDS 25.0%
!! CTATTCTTTTCAGATATCAT+AGG + chr5.4:47356994-47357013 None:intergenic 25.0%
!! TACCATCTTGAAGAAATTTT+GGG - chr5.4:47357143-47357162 MS.gene010311:CDS 25.0%
!! TTACCATCTTGAAGAAATTT+TGG - chr5.4:47357142-47357161 MS.gene010311:CDS 25.0%
!! TTCATTTCTTTTGTTGCTAA+GGG - chr5.4:47357470-47357489 MS.gene010311:CDS 25.0%
!! TTCGCAACTTTAAAAGAATT+TGG + chr5.4:47355236-47355255 None:intergenic 25.0%
!! TTCTTCTGTTTTGTATGTTT+AGG + chr5.4:47357252-47357271 None:intergenic 25.0%
!! TTTTGACATATTACATGATC+TGG - chr5.4:47356789-47356808 MS.gene010311:intron 25.0%
!!! ATTTTAACATAAGATGGAAG+GGG + chr5.4:47354919-47354938 None:intergenic 25.0%
!!! CTAATCTGTTTTGAATAAGA+GGG + chr5.4:47354955-47354974 None:intergenic 25.0%
!!! TAATCTGTTTTGAATAAGAG+GGG + chr5.4:47354954-47354973 None:intergenic 25.0%
!!! TCTAATCTGTTTTGAATAAG+AGG + chr5.4:47354956-47354975 None:intergenic 25.0%
!!! TTTTGGGAATTTAGATGATT+AGG - chr5.4:47357360-47357379 MS.gene010311:CDS 25.0%
AAAGACATATTATCAGCCTA+AGG - chr5.4:47355904-47355923 MS.gene010311:CDS 30.0%
AAATATTAGTTGGAATTCGC+AGG - chr5.4:47355639-47355658 MS.gene010311:CDS 30.0%
AAGAAAATAAATTGCGAACC+AGG + chr5.4:47357548-47357567 None:intergenic 30.0%
AAGACATATTATCAGCCTAA+GGG - chr5.4:47355905-47355924 MS.gene010311:CDS 30.0%
ATACAAAACAGAAGAAGCAA+AGG - chr5.4:47357256-47357275 MS.gene010311:CDS 30.0%
ATACAAACATAGTCAATCCT+TGG - chr5.4:47357819-47357838 MS.gene010311:intron 30.0%
ATCTGAAAAGAATAGCATAG+TGG - chr5.4:47356999-47357018 MS.gene010311:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG - chr5.4:47355951-47355970 MS.gene010311:CDS 30.0%
CAATATCAATCCCACAAATT+TGG + chr5.4:47354846-47354865 None:intergenic 30.0%
CATGAAAACAACAGAATCTT+TGG + chr5.4:47355603-47355622 None:intergenic 30.0%
CTTAGCAACAAAAGAAATGA+AGG + chr5.4:47357471-47357490 None:intergenic 30.0%
GGAATTTAGATGATTAGGAA+GGG - chr5.4:47357365-47357384 MS.gene010311:CDS 30.0%
GTGTAATTCAAGCATCTTTA+AGG + chr5.4:47354895-47354914 None:intergenic 30.0%
TAAAAGAATTTGGTACGTGA+GGG + chr5.4:47355226-47355245 None:intergenic 30.0%
TAACTTAGTAGCATCTAAAG+AGG - chr5.4:47356477-47356496 MS.gene010311:intron 30.0%
TCTGGAAAAAATCTCACATA+TGG - chr5.4:47356807-47356826 MS.gene010311:intron 30.0%
TGAGGATTCTGATTCTAATT+TGG - chr5.4:47357179-47357198 MS.gene010311:CDS 30.0%
TTAAAAGAATTTGGTACGTG+AGG + chr5.4:47355227-47355246 None:intergenic 30.0%
TTTGAATTCAGTGGAATTAG+CGG - chr5.4:47355724-47355743 MS.gene010311:CDS 30.0%
! ACATTTGGAACTTATCCTAT+AGG + chr5.4:47356174-47356193 None:intergenic 30.0%
! ACCATCTTGAAGAAATTTTG+GGG - chr5.4:47357144-47357163 MS.gene010311:CDS 30.0%
! CATTGAATGTGGAATTTGTT+GGG + chr5.4:47357942-47357961 None:intergenic 30.0%
! CATTTGGAACTTATCCTATA+GGG + chr5.4:47356173-47356192 None:intergenic 30.0%
! CTTCATTTCTTTTGTTGCTA+AGG - chr5.4:47357469-47357488 MS.gene010311:CDS 30.0%
! GTTTGATAATTGTGTTGAAG+TGG - chr5.4:47356068-47356087 MS.gene010311:CDS 30.0%
! TCTTTTGTGTAAAAAGGTTC+AGG - chr5.4:47357515-47357534 MS.gene010311:CDS 30.0%
!! ATTGAATGTGGAATTTGTTG+GGG + chr5.4:47357941-47357960 None:intergenic 30.0%
!! TATTGTGAGAGTTTTGACAA+AGG + chr5.4:47355390-47355409 None:intergenic 30.0%
!! TGCAGTATGATTTTGCTTTT+GGG - chr5.4:47357344-47357363 MS.gene010311:CDS 30.0%
AAAAGAATTTGGTACGTGAG+GGG + chr5.4:47355225-47355244 None:intergenic 35.0%
AAAGAAATGAAGGAAGCCTA+AGG + chr5.4:47357461-47357480 None:intergenic 35.0%
AAATTCCACATTCAATGGCA+GGG - chr5.4:47357945-47357964 MS.gene010311:intron 35.0%
AACAAGATTGTTTCCATCGT+CGG + chr5.4:47355366-47355385 None:intergenic 35.0%
ACCCCAAAATTTCTTCAAGA+TGG + chr5.4:47357148-47357167 None:intergenic 35.0%
ACCTTAAAAACCAAGAATGG+GGG - chr5.4:47355507-47355526 MS.gene010311:intron 35.0%
AGACATATTATCAGCCTAAG+GGG - chr5.4:47355906-47355925 MS.gene010311:CDS 35.0%
AGTTGCTTCTGTTACAGAAA+AGG - chr5.4:47356606-47356625 MS.gene010311:intron 35.0%
ATTATCCAACAGCTAGGAAT+TGG - chr5.4:47355774-47355793 MS.gene010311:CDS 35.0%
CAAAAGCAAAATCATACTGC+AGG + chr5.4:47357345-47357364 None:intergenic 35.0%
CCAACAAATTCCACATTCAA+TGG - chr5.4:47357940-47357959 MS.gene010311:intron 35.0%
CCATTGAATGTGGAATTTGT+TGG + chr5.4:47357943-47357962 None:intergenic 35.0%
CGACCTTAAAAACCAAGAAT+GGG - chr5.4:47355505-47355524 MS.gene010311:intron 35.0%
CGCTTTATCATAGGTTAACT+GGG - chr5.4:47357775-47357794 MS.gene010311:intron 35.0%
CTCTCATAACATAGTTGTTC+AGG - chr5.4:47356642-47356661 MS.gene010311:intron 35.0%
GACCTTAAAAACCAAGAATG+GGG - chr5.4:47355506-47355525 MS.gene010311:intron 35.0%
GCTATCAACAAGCATAATGT+TGG - chr5.4:47356520-47356539 MS.gene010311:intron 35.0%
GGAAAAAATCTCACATATGG+AGG - chr5.4:47356810-47356829 MS.gene010311:intron 35.0%
GGGAATTTAGATGATTAGGA+AGG - chr5.4:47357364-47357383 MS.gene010311:CDS 35.0%
TAAATTGCGAACCAGGATTA+AGG + chr5.4:47357541-47357560 None:intergenic 35.0%
TAGATGCTACTAAGTTACCA+CGG + chr5.4:47356474-47356493 None:intergenic 35.0%
TAGATTTACCCTTCTTAGAG+CGG + chr5.4:47357317-47357336 None:intergenic 35.0%
TCAAGATCCTACTTAGCAAT+AGG + chr5.4:47357644-47357663 None:intergenic 35.0%
TCGCTTTATCATAGGTTAAC+TGG - chr5.4:47357774-47357793 MS.gene010311:intron 35.0%
TCTGCCGGAAAAATATTAGT+TGG - chr5.4:47355629-47355648 MS.gene010311:CDS 35.0%
TTATCATAGGTTAACTGGGA+TGG - chr5.4:47357779-47357798 MS.gene010311:intron 35.0%
TTGGCATATCTGTGCAAAAT+TGG - chr5.4:47358083-47358102 MS.gene010311:intron 35.0%
TTGGTTCATTGGACCTATTA+TGG + chr5.4:47358042-47358061 None:intergenic 35.0%
TTGTGTATTATCCAACAGCT+AGG - chr5.4:47355768-47355787 MS.gene010311:CDS 35.0%
! AGAGAAGTGTAAGTAGTGAT+TGG + chr5.4:47357045-47357064 None:intergenic 35.0%
! AGGCTTTATGCCAAATTTGT+GGG - chr5.4:47354833-47354852 MS.gene010311:CDS 35.0%
! ATTCAAGCATCTTTAAGGAG+AGG + chr5.4:47354890-47354909 None:intergenic 35.0%
! CTGCAGTATGATTTTGCTTT+TGG - chr5.4:47357343-47357362 MS.gene010311:CDS 35.0%
! GGAAATGATAATGTTGCAGA+TGG + chr5.4:47356234-47356253 None:intergenic 35.0%
! GTTTTCATGAAAAACTCTGC+CGG - chr5.4:47355614-47355633 MS.gene010311:CDS 35.0%
! TTCAAGCATCTTTAAGGAGA+GGG + chr5.4:47354889-47354908 None:intergenic 35.0%
!! ATAAATACCCGCTCTAAGAA+GGG - chr5.4:47357306-47357325 MS.gene010311:CDS 35.0%
!! GACTTAACTTGCTTCAATAG+TGG - chr5.4:47356366-47356385 MS.gene010311:CDS 35.0%
!! TTGAATGTGGAATTTGTTGG+GGG + chr5.4:47357940-47357959 None:intergenic 35.0%
!!! CCATTCTTGGTTTTTAAGGT+CGG + chr5.4:47355507-47355526 None:intergenic 35.0%
AAACATAGTCAATCCTTGGC+AGG - chr5.4:47357823-47357842 MS.gene010311:intron 40.0%
AAACTCAGTAGACTACGTAC+TGG - chr5.4:47355542-47355561 MS.gene010311:intron 40.0%
AAAGAATTTGGTACGTGAGG+GGG + chr5.4:47355224-47355243 None:intergenic 40.0%
AAGCACTCCTATTGCTAAGT+AGG - chr5.4:47357634-47357653 MS.gene010311:intron 40.0%
AAGCATAATGTTGGCAAGCA+TGG - chr5.4:47356529-47356548 MS.gene010311:intron 40.0%
AGATGCTACTAAGTTACCAC+GGG + chr5.4:47356473-47356492 None:intergenic 40.0%
AGATTCCCAACCAATCTTCA+TGG + chr5.4:47355837-47355856 None:intergenic 40.0%
AGATTTACCCTTCTTAGAGC+GGG + chr5.4:47357316-47357335 None:intergenic 40.0%
AGCATAATGTTGGCAAGCAT+GGG - chr5.4:47356530-47356549 MS.gene010311:intron 40.0%
ATAGTTGTTCAGGCAGATCA+AGG - chr5.4:47356652-47356671 MS.gene010311:intron 40.0%
ATCTATCAAGTGCAAGAACG+AGG + chr5.4:47356256-47356275 None:intergenic 40.0%
ATGATGCCATGAAGATTGGT+TGG - chr5.4:47355828-47355847 MS.gene010311:CDS 40.0%
ATGTTGCTAAGTAACCGATG+TGG - chr5.4:47354990-47355009 MS.gene010311:CDS 40.0%
CAAATTCCACATTCAATGGC+AGG - chr5.4:47357944-47357963 MS.gene010311:intron 40.0%
CATAGGATCCAAGCTTGAAA+AGG + chr5.4:47356977-47356996 None:intergenic 40.0%
CCGACCTTAAAAACCAAGAA+TGG - chr5.4:47355504-47355523 MS.gene010311:intron 40.0%
CTGGAATTTAACTGAGAGTG+CGG - chr5.4:47355700-47355719 MS.gene010311:CDS 40.0%
GAATTGGTGTGATTTCGTGA+TGG - chr5.4:47355790-47355809 MS.gene010311:CDS 40.0%
GATCTTTCCAAAGCTTGACT+TGG - chr5.4:47355457-47355476 MS.gene010311:intron 40.0%
GGGTATTTATGCCTTCAGAA+GGG + chr5.4:47357296-47357315 None:intergenic 40.0%
GGTATTTATGCCTTCAGAAG+GGG + chr5.4:47357295-47357314 None:intergenic 40.0%
GTAGATGATGCCATGAAGAT+TGG - chr5.4:47355824-47355843 MS.gene010311:CDS 40.0%
TAGTCTACTGAGTTTCACGT+AGG + chr5.4:47355537-47355556 None:intergenic 40.0%
TATACATAGTCGGTACACCT+GGG + chr5.4:47355201-47355220 None:intergenic 40.0%
TATATTTCAACCACGAACCC+AGG - chr5.4:47355181-47355200 MS.gene010311:intron 40.0%
TCTATCAAGTGCAAGAACGA+GGG + chr5.4:47356255-47356274 None:intergenic 40.0%
TGAAGCTGCTATTCACACTA+CGG - chr5.4:47356903-47356922 MS.gene010311:intron 40.0%
TGTTGCTAAAGGATAGCCTT+AGG - chr5.4:47357442-47357461 MS.gene010311:CDS 40.0%
TGTTGCTAAGTAACCGATGT+GGG - chr5.4:47354991-47355010 MS.gene010311:CDS 40.0%
TTGGGGTGATTATCTTGATG+AGG - chr5.4:47357161-47357180 MS.gene010311:CDS 40.0%
! ACATCGGTTACTTAGCAACA+TGG + chr5.4:47354991-47355010 None:intergenic 40.0%
! ACTCACTGATATGAGAGCAT+TGG + chr5.4:47355037-47355056 None:intergenic 40.0%
! ATGAAGATTGGTTGGGAATC+TGG - chr5.4:47355836-47355855 MS.gene010311:CDS 40.0%
! ATTGTTCCCTGCTAAGTTGA+TGG + chr5.4:47357217-47357236 None:intergenic 40.0%
! CAGGCTTTATGCCAAATTTG+TGG - chr5.4:47354832-47354851 MS.gene010311:CDS 40.0%
! GTGTAAGTAGTGATTGGTTG+TGG + chr5.4:47357039-47357058 None:intergenic 40.0%
! TCAACATAAGGTACCAGCAA+TGG - chr5.4:47356747-47356766 MS.gene010311:intron 40.0%
! TGATGCCATGAAGATTGGTT+GGG - chr5.4:47355829-47355848 MS.gene010311:CDS 40.0%
! TTTTGTTGAGGTGAGGTTGA+CGG + chr5.4:47357891-47357910 None:intergenic 40.0%
!! ACCCCCATTCTTGGTTTTTA+AGG + chr5.4:47355511-47355530 None:intergenic 40.0%
!! CATAAATACCCGCTCTAAGA+AGG - chr5.4:47357305-47357324 MS.gene010311:CDS 40.0%
!! CTCACTGATATGAGAGCATT+GGG + chr5.4:47355036-47355055 None:intergenic 40.0%
!! CTGTTTTGAATAAGAGGGGA+GGG + chr5.4:47354950-47354969 None:intergenic 40.0%
!! TAGCAATAGGAGTGCTTAGA+AGG + chr5.4:47357631-47357650 None:intergenic 40.0%
!! TCTGTTTTGAATAAGAGGGG+AGG + chr5.4:47354951-47354970 None:intergenic 40.0%
!! TGAATGTGGAATTTGTTGGG+GGG + chr5.4:47357939-47357958 None:intergenic 40.0%
!! TGATTTCGTGATGGATGCTA+AGG - chr5.4:47355799-47355818 MS.gene010311:CDS 40.0%
!! TGTTTTGAATAAGAGGGGAG+GGG + chr5.4:47354949-47354968 None:intergenic 40.0%
!! TTGTTCCCTGCTAAGTTGAT+GGG + chr5.4:47357216-47357235 None:intergenic 40.0%
AAAGGTTCAGGCCTTAATCC+TGG - chr5.4:47357527-47357546 MS.gene010311:CDS 45.0%
AACATGAGCTAGTCCCACAT+CGG + chr5.4:47355007-47355026 None:intergenic 45.0%
AATCTCACATATGGAGGCCT+TGG - chr5.4:47356816-47356835 MS.gene010311:intron 45.0%
AATGAACCAAGCACCAAGTG+GGG - chr5.4:47358052-47358071 MS.gene010311:intron 45.0%
ACAACCCCATCAACTTAGCA+GGG - chr5.4:47357208-47357227 MS.gene010311:CDS 45.0%
ACAGTAGAGATTGTCCCCTT+AGG + chr5.4:47355923-47355942 None:intergenic 45.0%
ACCTCAAGAATGCGCCAATT+AGG + chr5.4:47355968-47355987 None:intergenic 45.0%
AGCCTGCCATGATAAGCAAA+GGG + chr5.4:47357402-47357421 None:intergenic 45.0%
ATCAAGTCAGCGCATTCATG+AGG + chr5.4:47355440-47355459 None:intergenic 45.0%
CAATGAACCAAGCACCAAGT+GGG - chr5.4:47358051-47358070 MS.gene010311:intron 45.0%
CAGTCACAATTAGGAACTCC+GGG - chr5.4:47358113-47358132 MS.gene010311:intron 45.0%
CCAAACTTTGGCAGTCCTTA+CGG - chr5.4:47357995-47358014 MS.gene010311:intron 45.0%
CCAAAGTTTGGCTATGTGTC+AGG + chr5.4:47357986-47358005 None:intergenic 45.0%
CCAGTCACAATTAGGAACTC+CGG - chr5.4:47358112-47358131 MS.gene010311:intron 45.0%
CCTCAAGAATGCGCCAATTA+GGG + chr5.4:47355967-47355986 None:intergenic 45.0%
CCTGACACATAGCCAAACTT+TGG - chr5.4:47357983-47358002 MS.gene010311:intron 45.0%
CGGGTATTTATGCCTTCAGA+AGG + chr5.4:47357297-47357316 None:intergenic 45.0%
GACTTCTCCAAGTCAAGCTT+TGG + chr5.4:47355467-47355486 None:intergenic 45.0%
GATAAACTCTCAGCAAGTCC+AGG + chr5.4:47357917-47357936 None:intergenic 45.0%
GCAAAATCATACTGCAGGAG+TGG + chr5.4:47357340-47357359 None:intergenic 45.0%
GTATACATAGTCGGTACACC+TGG + chr5.4:47355202-47355221 None:intergenic 45.0%
TACACCAGATGCTAAGTCAG+TGG + chr5.4:47356869-47356888 None:intergenic 45.0%
TCACACCAATTCCTAGCTGT+TGG + chr5.4:47355782-47355801 None:intergenic 45.0%
TGACTATGCTCTCAGTGATG+AGG - chr5.4:47355261-47355280 MS.gene010311:intron 45.0%
TGGTGACTGCACAACAAACT+TGG - chr5.4:47356942-47356961 MS.gene010311:CDS 45.0%
TGTTGCTAAGGGATAGCCTT+AGG - chr5.4:47357481-47357500 MS.gene010311:CDS 45.0%
TGTTGTGCAGTCACCAAACT+TGG + chr5.4:47356938-47356957 None:intergenic 45.0%
TTGCAGCCCTTTGCTTATCA+TGG - chr5.4:47357393-47357412 MS.gene010311:CDS 45.0%
TTGGAAACTTCAACCTGCCA+AGG + chr5.4:47357839-47357858 None:intergenic 45.0%
! AACACTTTTCTGAGCTGCAG+CGG + chr5.4:47357124-47357143 None:intergenic 45.0%
! AAGTCCAGGAGCTTTTGTTG+AGG + chr5.4:47357903-47357922 None:intergenic 45.0%
! ACCAAGTGGGGTATCTTCAT+TGG - chr5.4:47358064-47358083 MS.gene010311:intron 45.0%
! AGCATTGAGTCAGAACCGTA+AGG + chr5.4:47358013-47358032 None:intergenic 45.0%
! AGTAGTGATTGGTTGTGGCA+TGG + chr5.4:47357034-47357053 None:intergenic 45.0%
! GGAATTAGCGGACAAGAACA+TGG - chr5.4:47355736-47355755 MS.gene010311:CDS 45.0%
! GGTGTTGCATTTACCATTGC+TGG + chr5.4:47356763-47356782 None:intergenic 45.0%
! GTAGTGATTGGTTGTGGCAT+GGG + chr5.4:47357033-47357052 None:intergenic 45.0%
! TCACTGAGAGCATAGTCACA+CGG + chr5.4:47355259-47355278 None:intergenic 45.0%
! TGTTAGCACAGTGTTGAGAC+CGG - chr5.4:47357086-47357105 MS.gene010311:CDS 45.0%
!! AAGCTAGCTTTGTGCCCTAT+AGG - chr5.4:47356156-47356175 MS.gene010311:CDS 45.0%
!! AGAAGGTATTGGCCAGTTAC+CGG + chr5.4:47355310-47355329 None:intergenic 45.0%
!! AGTTACCACGGGCTATCTTT+TGG + chr5.4:47356462-47356481 None:intergenic 45.0%
!! TGATGCTGCCTTTTCAAGCT+TGG - chr5.4:47356966-47356985 MS.gene010311:CDS 45.0%
!! TGTTCCCTGCTAAGTTGATG+GGG + chr5.4:47357215-47357234 None:intergenic 45.0%
!!! GAGTGCGGTTTTGAATTCAG+TGG - chr5.4:47355715-47355734 MS.gene010311:CDS 45.0%
ACCATTCATCACCTCAGCCA+AGG + chr5.4:47356836-47356855 None:intergenic 50.0%
ACGGATGCTAGCTCCAAGTT+TGG - chr5.4:47356922-47356941 MS.gene010311:intron 50.0%
AGCCCTTTGCTTATCATGGC+AGG - chr5.4:47357397-47357416 MS.gene010311:CDS 50.0%
ATTGGCCAGTTACCGGAGTA+AGG + chr5.4:47355303-47355322 None:intergenic 50.0%
CCAATGAACCAAGCACCAAG+TGG - chr5.4:47358050-47358069 MS.gene010311:intron 50.0%
CCCTAATTGGCGCATTCTTG+AGG - chr5.4:47355964-47355983 MS.gene010311:CDS 50.0%
CCGGAGTTCCTAATTGTGAC+TGG + chr5.4:47358115-47358134 None:intergenic 50.0%
CCGTAAGGACTGCCAAAGTT+TGG + chr5.4:47357998-47358017 None:intergenic 50.0%
CTCACCTCAACAAAAGCTCC+TGG - chr5.4:47357896-47357915 MS.gene010311:intron 50.0%
GACAACCCCATCAACTTAGC+AGG - chr5.4:47357207-47357226 MS.gene010311:CDS 50.0%
GAGCCTGCCATGATAAGCAA+AGG + chr5.4:47357403-47357422 None:intergenic 50.0%
GCCAATGAAGATACCCCACT+TGG + chr5.4:47358068-47358087 None:intergenic 50.0%
GGTCTCAACACTGTGCTAAC+AGG + chr5.4:47357087-47357106 None:intergenic 50.0%
GGTCTGCACCAGTCACAATT+AGG - chr5.4:47358104-47358123 MS.gene010311:intron 50.0%
TACCGGAGTAAGGAGCAGTT+TGG + chr5.4:47355293-47355312 None:intergenic 50.0%
TGCCAAACTGCTCCTTACTC+CGG - chr5.4:47355288-47355307 MS.gene010311:intron 50.0%
! AGTCGAAGGTGCAGGTATGT+TGG + chr5.4:47357858-47357877 None:intergenic 50.0%
! CAGGAGCTTTTGTTGAGGTG+AGG + chr5.4:47357898-47357917 None:intergenic 50.0%
! CGTGAGGGGGTATACATAGT+CGG + chr5.4:47355211-47355230 None:intergenic 50.0%
! GGAGGAGAAGGAGAAGGTAT+TGG + chr5.4:47355321-47355340 None:intergenic 50.0%
! TCAGTAGACTACGTACTGGC+TGG - chr5.4:47355546-47355565 MS.gene010311:intron 50.0%
!! AAGATACCCCACTTGGTGCT+TGG + chr5.4:47358061-47358080 None:intergenic 50.0%
!! CCACTTGGTGCTTGGTTCAT+TGG + chr5.4:47358053-47358072 None:intergenic 50.0%
!! GACTCCACTGACTTAGCATC+TGG - chr5.4:47356862-47356881 MS.gene010311:intron 50.0%
!! GGGCTATCTTTTGGCACTGA+TGG + chr5.4:47356453-47356472 None:intergenic 50.0%
!! TTGTTTCCATCGTCGGCTTC+TGG + chr5.4:47355359-47355378 None:intergenic 50.0%
AACTTCCCTGCAGCTGAAGC+GGG - chr5.4:47355668-47355687 MS.gene010311:CDS 55.0%
ACAGGAGGAGGAGAAGGAGA+AGG + chr5.4:47355327-47355346 None:intergenic 55.0%
ACATATGGAGGCCTTGGCTG+AGG - chr5.4:47356822-47356841 MS.gene010311:intron 55.0%
ACGGTGAAGGACGAAGTCGA+AGG + chr5.4:47357872-47357891 None:intergenic 55.0%
CAACTTCCCTGCAGCTGAAG+CGG - chr5.4:47355667-47355686 MS.gene010311:CDS 55.0%
CAGTGCCAAAAGATAGCCCG+TGG - chr5.4:47356454-47356473 MS.gene010311:intron 55.0%
CATTCAATGGCAGGGTCGAG+TGG - chr5.4:47357953-47357972 MS.gene010311:intron 55.0%
CTCGACCCTGCCATTGAATG+TGG + chr5.4:47357953-47357972 None:intergenic 55.0%
CTGCTCCTTACTCCGGTAAC+TGG - chr5.4:47355295-47355314 MS.gene010311:intron 55.0%
GCAGCGGATGGAGTAAGAAC+CGG + chr5.4:47357108-47357127 None:intergenic 55.0%
GCCTTGGCTGAGGTGATGAA+TGG - chr5.4:47356832-47356851 MS.gene010311:intron 55.0%
GGAACAACAGGAGGAGGAGA+AGG + chr5.4:47355333-47355352 None:intergenic 55.0%
TCTCCTCCTCCTGTTGTTCC+TGG - chr5.4:47355333-47355352 MS.gene010311:intron 55.0%
TGAGGTGAGGTTGACGGTGA+AGG + chr5.4:47357885-47357904 None:intergenic 55.0%
TTCTGGCCAGGAACAACAGG+AGG + chr5.4:47355342-47355361 None:intergenic 55.0%
TTTCACCCGCTTCAGCTGCA+GGG + chr5.4:47355676-47355695 None:intergenic 55.0%
! AAGGACGAAGTCGAAGGTGC+AGG + chr5.4:47357866-47357885 None:intergenic 55.0%
! CTTTTCTGAGCTGCAGCGGA+TGG + chr5.4:47357120-47357139 None:intergenic 55.0%
!! AAGCTTGCTGCCCCTTCTGA+AGG - chr5.4:47357282-47357301 MS.gene010311:CDS 55.0%
AGTCGGTACACCTGGGTTCG+TGG + chr5.4:47355194-47355213 None:intergenic 60.0%
CGCTTCAGCTGCAGGGAAGT+TGG + chr5.4:47355669-47355688 None:intergenic 60.0%
CGTAGGTGCACCCCCATTCT+TGG + chr5.4:47355520-47355539 None:intergenic 60.0%
CTGAAGCGGGTGAAAGAGGC+TGG - chr5.4:47355681-47355700 MS.gene010311:CDS 60.0%
CTTTCACCCGCTTCAGCTGC+AGG + chr5.4:47355677-47355696 None:intergenic 60.0%
GCAGCTGAAGCGGGTGAAAG+AGG - chr5.4:47355677-47355696 MS.gene010311:CDS 60.0%
GGCTTCTGGCCAGGAACAAC+AGG + chr5.4:47355345-47355364 None:intergenic 60.0%
TGGCCAGGAACAACAGGAGG+AGG + chr5.4:47355339-47355358 None:intergenic 60.0%
TCCTGGCCAGAAGCCGACGA+TGG - chr5.4:47355350-47355369 MS.gene010311:intron 65.0%
!! TCCATCGTCGGCTTCTGGCC+AGG + chr5.4:47355354-47355373 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 47354765 47358148 47354765 ID=MS.gene010311
chr5.4 mRNA 47354765 47358148 47354765 ID=MS.gene010311.t1;Parent=MS.gene010311
chr5.4 exon 47358139 47358148 47358139 ID=MS.gene010311.t1.exon1;Parent=MS.gene010311.t1
chr5.4 CDS 47358139 47358148 47358139 ID=cds.MS.gene010311.t1;Parent=MS.gene010311.t1
chr5.4 exon 47356928 47357553 47356928 ID=MS.gene010311.t1.exon2;Parent=MS.gene010311.t1
chr5.4 CDS 47356928 47357553 47356928 ID=cds.MS.gene010311.t1;Parent=MS.gene010311.t1
chr5.4 exon 47355566 47356456 47355566 ID=MS.gene010311.t1.exon3;Parent=MS.gene010311.t1
chr5.4 CDS 47355566 47356456 47355566 ID=cds.MS.gene010311.t1;Parent=MS.gene010311.t1
chr5.4 exon 47355365 47355460 47355365 ID=MS.gene010311.t1.exon4;Parent=MS.gene010311.t1
chr5.4 CDS 47355365 47355460 47355365 ID=cds.MS.gene010311.t1;Parent=MS.gene010311.t1
chr5.4 exon 47354939 47355125 47354939 ID=MS.gene010311.t1.exon5;Parent=MS.gene010311.t1
chr5.4 CDS 47354939 47355125 47354939 ID=cds.MS.gene010311.t1;Parent=MS.gene010311.t1
chr5.4 exon 47354765 47354865 47354765 ID=MS.gene010311.t1.exon6;Parent=MS.gene010311.t1
chr5.4 CDS 47354765 47354865 47354765 ID=cds.MS.gene010311.t1;Parent=MS.gene010311.t1
Gene Sequence

>MS.gene010311

ATGTTTAACGGTTAGAAAGATGAAAAAATACAATTAGAAGTTATATGAAGAATCATATAATATTTTTCAGGCTTTATGCCAAATTTGTGGGATTGATATTGAGATATAGTAAATTACACTTCCCTCTCCTTAAAGATGCTTGAATTACACTCCCCTTCCATCTTATGTTAAAATATATACTCCCCTCCCCTCTTATTCAAAACAGATTAGAAATTTTTTCACTCCATGTTGCTAAGTAACCGATGTGGGACTAGCTCATGTTATATATCCCAATGCTCTCATATCAGTGAGTTTCAACTAGTATATCTCATCTTTTTGTAAAAAAAAATATTATTTTTATTGATAGTATTAATATTTGCTTTGTTTGTTTTATATTTTTACTTAATTAATTTTGTTATCCACAATATACCGATTATTATATTTCAACCACGAACCCAGGTGTACCGACTATGTATACCCCCTCACGTACCAAATTCTTTTAAAGTTGCGAACCGTGTGACTATGCTCTCAGTGATGAGGTTTTTGCCAAACTGCTCCTTACTCCGGTAACTGGCCAATACCTTCTCCTTCTCCTCCTCCTGTTGTTCCTGGCCAGAAGCCGACGATGGAAACAATCTTGTTTCCTTTGTCAAAACTCTCACAATAACTGATACTAAATCTGTATTCAAGATACCTCATGAATGCGCTGACTTGATCTTTCCAAAGCTTGACTTGGAGAAGTCTCAAAACATCACTGTCACCGACCTTAAAAACCAAGAATGGGGGTGCACCTACGTGAAACTCAGTAGACTACGTACTGGCTGGTCTCACTTTGTGAAAGAGAAGAAACTCGTCGCCAAAGATTCTGTTGTTTTCATGAAAAACTCTGCCGGAAAAATATTAGTTGGAATTCGCAGGAACACCAACTTCCCTGCAGCTGAAGCGGGTGAAAGAGGCTGGAATTTAACTGAGAGTGCGGTTTTGAATTCAGTGGAATTAGCGGACAAGAACATGGAGTTTGAAGTTGTGTATTATCCAACAGCTAGGAATTGGTGTGATTTCGTGATGGATGCTAAGGTTGTAGATGATGCCATGAAGATTGGTTGGGAATCTGGAATGAGAGTCAAACTTTCTTTGAAGAAAGATGAATCTTCAAATTCAAAGACATATTATCAGCCTAAGGGGACAATCTCTACTGTCTTTAATTATTCTTCTAATGTCCCTAATTGGCGCATTCTTGAGGTGCATATGCTTTCTTATTTTAATTGCATATTGTTATCAAGTTTATAGTTTTATTAGAGTTGAATCCTTAATTTTATGATTTGTTTGATAATTGTGTTGAAGTGGATACATGATTCATTTAGATGTTGAAACAATAATTAGATGAAATAGATATACAAAATTTGGAGAGTAAGCTAGCTTTGTGCCCTATAGGATAAGTTCCAAATGTAATTCGAAAATTTTGAGACAGAAAATCATTAGAGTCTCCATCTGCAACATTATCATTTCCCTCGTTCTTGCACTTGATAGATATCTCATGACACTTAATGTATACTTTAGATAATCAGATTACATTTTAGAATCGATTGTTTGTTATTATAGACTTAATCGATTATTTGTATGACTTAACTTGCTTCAATAGTGGCAATAGTGTATGAAAAGCACCCATTATTTTGCAATAATTGTAAGTCTATTGGACACTCTATCCATCAGTGCCAAAAGATAGCCCGTGGTAACTTAGTAGCATCTAAAGAGGTGAAAAAGAATTTGTCATCTGCTATCAACAAGCATAATGTTGGCAAGCATGGGTTAAATTTGACTGAAAAAAATAAGGTTGCAGCTAGCTTTCATACTCATCAAGAAGTTGCTTCTGTTACAGAAAAGGATTCTGATACTATCTCTCATAACATAGTTGTTCAGGCAGATCAAGGCATTGATTCTGCACAAATTGTTTCTGATGTAAACTATAATACTATGAGACATAGGACAGATAACTCTACTATTCAACATAAGGTACCAGCAATGGTAAATGCAACACCAAACTCTTTTGACATATTACATGATCTGGAAAAAATCTCACATATGGAGGCCTTGGCTGAGGTGATGAATGGTAATCTAGACTCCACTGACTTAGCATCTGGTGTAGCTAACAATGTGAATGAAGCTGCTATTCACACTACGGATGCTAGCTCCAAGTTTGGTGACTGCACAACAAACTTGGATGATGCTGCCTTTTCAAGCTTGGATCCTATGATATCTGAAAAGAATAGCATAGTGGTGCAGCATCCCATGCCACAACCAATCACTACTTACACTTCTCTGACAAGTGACAAGATCAAGCCTGTTAGCACAGTGTTGAGACCGGTTCTTACTCCATCCGCTGCAGCTCAGAAAAGTGTTACCATCTTGAAGAAATTTTGGGGTGATTATCTTGATGAGGATTCTGATTCTAATTTGGATTTGGACAACCCCATCAACTTAGCAGGGAACAATTCTATCTTAACTCCTAAACATACAAAACAGAAGAAGCAAAGGAATAAGCTTGCTGCCCCTTCTGAAGGCATAAATACCCGCTCTAAGAAGGGTAAATCTACCACTCCTGCAGTATGATTTTGCTTTTGGGAATTTAGATGATTAGGAAGGGTGTGTTTGCAGCCCTTTGCTTATCATGGCAGGCTCCTCTCAGTTTTTGTTTTTTTGTTGCTAAAGGATAGCCTTAGGCTTCCTTCATTTCTTTTGTTGCTAAGGGATAGCCTTAGGCTTCCTTCATTTCTTTTGTGTAAAAAGGTTCAGGCCTTAATCCTGGTTCGCAATTTATTTTCTTAGCTTTGATCAAAAAACTTGCTTCAATAGTGATATTCATTAAGAAAACGAAGTTAAAGAACCTTCTAAGCACTCCTATTGCTAAGTAGGATCTTGATTTTGACTCATGTTATCAATTGATAAGAACCTTTGGATGTTTAAATACAAATATATATTAAGCAATCAAATATTTTAAATAATTGATATTGATTCTTACCATTTTTTTTATCGCTTTATCATAGGTTAACTGGGATGGACCTAAAATCTTGCAAAATACAAACATAGTCAATCCTTGGCAGGTTGAAGTTTCCAACATACCTGCACCTTCGACTTCGTCCTTCACCGTCAACCTCACCTCAACAAAAGCTCCTGGACTTGCTGAGAGTTTATCCCCCCAACAAATTCCACATTCAATGGCAGGGTCGAGTGGTACTTTGCCTGACACATAGCCAAACTTTGGCAGTCCTTACGGTTCTGACTCAATGCTTTATAACCATAATAGGTCCAATGAACCAAGCACCAAGTGGGGTATCTTCATTGGCATATCTGTGCAAAATTGGTCTGCACCAGTCACAATTAGGAACTCCGGGAAAATTAGTTTAA
Protein sequence

>MS.gene010311.t1

MFNEADDGNNLVSFVKTLTITDTKSVFKIPHECADLIFPKLDLEKSQNITVTDLKNQEWGCTYVKLSRLRTGWSHFVKEKKLVAKDSVVFMKNSAGKILVGIRRNTNFPAAEAGERGWNLTESAVLNSVELADKNMEFEVVYYPTARNWCDFVMDAKVVDDAMKIGWESGMRVKLSLKKDESSNSKTYYQPKGTISTVFNYSSNVPNWRILECQKIARGNLVASKEVKKNLSSAINKHNVGKHGLNLTEKNKVAASFHTHQEVASVTEKDSDTISHNIVVQADQGIDSAQIVSDVNYNTMRHRTDNSTIQHKVPAMVNATPNSFDILHDLEKISHMEALAEVMNGNLDSTDLASGVANNVNEAAIHTTDASSKFGDCTTNLDDAAFSSLDPMISEKNSIVVQHPMPQPITTYTSLTSDKIKPVSTVLRPVLTPSAAAQKSVTILKKFWGDYLDEDSDSNLDLDNPINLAGNNSILTPKHTKQKKQRNKLAAPSEGINTRSKKGKSTTPADSLRLPSFLLLLRDSLRLPSFLLCKKVQALILVNWDGPKILQNTNIVNPWQVEVSNIPAPSTSSFTVNLTSTKAPGLAESLSPQQIPHSMAGSSGPMNQAPSGVSSLAYLCKIGLHQSQLGTPGKLV