AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010312

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010312.t1 MTR_5g061890 90.842 404 29 2 1 401 1 399 0.0 746
MS.gene010312.t1 MTR_5g060780 89.851 404 33 2 1 401 1 399 0.0 736
MS.gene010312.t1 MTR_5g460920 89.851 404 33 2 1 401 1 399 0.0 735
MS.gene010312.t1 MTR_5g060630 54.916 417 135 8 1 367 1 414 1.60e-133 393
MS.gene010312.t1 MTR_5g460740 54.916 417 135 8 1 367 1 414 1.60e-133 393
MS.gene010312.t1 MTR_5g061220 54.177 395 140 9 1 364 8 392 1.13e-129 383
MS.gene010312.t1 MTR_1g058210 53.806 381 140 10 1 364 1 362 4.60e-120 356
MS.gene010312.t1 MTR_2g024430 49.127 401 162 18 1 382 9 386 4.82e-104 317
MS.gene010312.t1 MTR_5g040880 49.558 339 146 9 8 335 7 331 2.85e-95 295
MS.gene010312.t1 MTR_5g040740 51.506 332 126 11 1 315 1 314 1.19e-89 277
MS.gene010312.t1 MTR_2g006270 45.052 384 180 14 6 374 7 374 7.27e-88 274
MS.gene010312.t1 MTR_5g082140 45.954 346 158 10 6 336 6 337 4.31e-87 272
MS.gene010312.t1 MTR_5g060770 53.094 307 88 7 61 365 7 259 1.49e-84 262
MS.gene010312.t1 MTR_2g006380 42.520 381 187 14 6 374 14 374 8.01e-84 263
MS.gene010312.t1 MTR_5g460910 42.092 392 116 12 52 401 14 336 7.40e-67 216
MS.gene010312.t1 MTR_5g062970 57.143 196 68 7 12 195 38 229 2.07e-62 201
MS.gene010312.t1 MTR_3g073420 34.921 378 184 10 9 336 16 381 6.46e-61 207
MS.gene010312.t1 MTR_1g064430 36.253 411 191 14 9 360 24 422 1.47e-57 200
MS.gene010312.t1 MTR_1g064430 36.253 411 191 14 9 360 37 435 2.14e-57 199
MS.gene010312.t1 MTR_1g094960 35.539 408 191 17 9 361 16 406 2.45e-57 198
MS.gene010312.t1 MTR_2g094570 34.828 379 186 12 9 336 8 376 6.05e-55 192
MS.gene010312.t1 MTR_2g094570 34.828 379 186 12 9 336 8 376 7.93e-55 192
MS.gene010312.t1 MTR_2g094570 34.828 379 186 12 9 336 64 432 1.77e-54 192
MS.gene010312.t1 MTR_4g058930 35.641 390 183 13 9 340 9 388 4.07e-53 187
MS.gene010312.t1 MTR_4g058930 35.641 390 183 13 9 340 9 388 4.78e-53 187
MS.gene010312.t1 MTR_7g101280 30.521 403 193 13 9 341 28 413 4.60e-48 173
MS.gene010312.t1 MTR_0041s0110 30.521 403 193 13 9 341 28 413 4.60e-48 173
MS.gene010312.t1 MTR_7g101275 32.381 315 175 11 22 314 2 300 5.56e-40 147
MS.gene010312.t1 MTR_5g062960 63.393 112 40 1 227 338 1 111 1.43e-39 140
MS.gene010312.t1 MTR_2g014770 29.639 388 229 15 4 364 43 413 7.58e-39 147
MS.gene010312.t1 MTR_2g014770 29.639 388 229 15 4 364 43 413 1.05e-38 147
MS.gene010312.t1 MTR_2g005240 28.238 386 232 10 12 371 23 389 1.51e-36 141
MS.gene010312.t1 MTR_4g021580 28.412 359 237 8 12 358 19 369 1.80e-36 140
MS.gene010312.t1 MTR_4g021580 28.412 359 237 8 12 358 19 369 2.28e-36 140
MS.gene010312.t1 MTR_0041s0120 31.173 324 179 11 22 314 2 312 7.27e-36 136
MS.gene010312.t1 MTR_7g062540 28.453 362 236 10 12 359 14 366 1.76e-35 138
MS.gene010312.t1 MTR_8g100050 28.448 348 210 9 12 335 59 391 4.20e-35 137
MS.gene010312.t1 MTR_8g100050 28.448 348 210 9 12 335 59 391 9.57e-35 136
MS.gene010312.t1 MTR_2g018690 29.651 344 219 10 9 335 20 357 2.44e-34 135
MS.gene010312.t1 MTR_4g088210 28.756 386 228 15 12 369 38 404 6.62e-34 134
MS.gene010312.t1 MTR_4g088210 28.756 386 228 15 12 369 38 404 6.97e-34 134
MS.gene010312.t1 MTR_8g027440 28.750 400 259 12 12 398 29 415 1.23e-33 133
MS.gene010312.t1 MTR_4g124900 28.406 345 220 10 9 335 20 355 1.07e-31 127
MS.gene010312.t1 MTR_4g124900 28.406 345 220 10 9 335 20 355 1.31e-31 127
MS.gene010312.t1 MTR_5g076270 29.326 341 218 10 12 335 24 358 2.93e-31 126
MS.gene010312.t1 MTR_2g043250 29.070 344 219 11 9 335 20 355 3.65e-31 126
MS.gene010312.t1 MTR_8g079492 28.249 354 210 12 9 335 20 356 4.51e-31 125
MS.gene010312.t1 MTR_8g079492 28.249 354 210 12 9 335 20 356 5.13e-31 125
MS.gene010312.t1 MTR_1g024025 29.912 341 215 11 12 335 43 376 9.09e-31 125
MS.gene010312.t1 MTR_3g064050 29.032 341 219 10 12 335 26 360 3.87e-30 123
MS.gene010312.t1 MTR_2g093740 29.219 397 235 16 12 376 56 438 8.62e-29 119
MS.gene010312.t1 MTR_2g093740 29.219 397 235 16 12 376 56 438 9.68e-29 119
MS.gene010312.t1 MTR_2g093740 29.219 397 235 16 12 376 56 438 1.17e-28 118
MS.gene010312.t1 MTR_4g060460 28.535 396 240 15 12 377 54 436 1.74e-27 115
MS.gene010312.t1 MTR_8g101360 28.409 352 213 11 9 335 28 365 7.74e-27 113
MS.gene010312.t1 MTR_4g060460 28.205 390 237 15 12 371 54 430 3.40e-26 111
MS.gene010312.t1 MTR_2g093740 28.293 410 235 17 12 376 56 451 1.03e-25 109
MS.gene010312.t1 MTR_2g093740 28.293 410 235 17 12 376 56 451 1.26e-25 109
MS.gene010312.t1 MTR_2g093740 28.293 410 235 17 12 376 56 451 1.66e-25 109
MS.gene010312.t1 MTR_8g079492 27.178 287 175 9 70 335 1 274 2.28e-20 94.0
MS.gene010312.t1 MTR_8g446900 27.181 298 173 12 61 335 12 288 3.53e-19 89.0
MS.gene010312.t1 MTR_2g093740 28.826 281 174 11 112 376 38 308 7.56e-16 79.7
MS.gene010312.t1 MTR_2g093740 28.826 281 174 11 112 376 38 308 7.76e-16 79.7
MS.gene010312.t1 MTR_5g046120 36.752 117 68 3 50 160 12 128 1.01e-14 70.9
MS.gene010312.t1 MTR_2g093740 27.551 294 174 12 112 376 38 321 5.50e-13 70.9
MS.gene010312.t1 MTR_2g093740 27.551 294 174 12 112 376 38 321 6.51e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010312.t1 AT1G77850 35.027 374 188 13 8 336 15 378 1.34e-61 209
MS.gene010312.t1 AT1G77850 35.027 374 188 13 8 336 15 378 1.51e-61 209
MS.gene010312.t1 AT4G30080 35.550 391 191 14 9 347 16 397 1.46e-52 186
MS.gene010312.t1 AT2G28350 33.756 394 186 14 9 340 7 387 3.93e-52 185
MS.gene010312.t1 AT2G28350 33.756 394 186 14 9 340 7 387 5.08e-52 185
MS.gene010312.t1 AT2G46530 30.055 366 230 8 12 361 21 376 1.98e-42 157
MS.gene010312.t1 AT2G46530 30.055 366 230 8 12 361 42 397 2.16e-42 157
MS.gene010312.t1 AT3G61830 30.145 345 219 8 12 342 25 361 2.60e-41 154
MS.gene010312.t1 AT1G34390 30.576 399 233 14 12 385 27 406 6.75e-39 147
MS.gene010312.t1 AT1G35520 31.463 410 219 17 12 386 27 409 1.17e-38 146
MS.gene010312.t1 AT1G59750 31.688 385 229 14 12 377 22 391 2.47e-38 146
MS.gene010312.t1 AT1G59750 31.688 385 229 14 12 377 22 391 2.47e-38 146
MS.gene010312.t1 AT1G59750 31.854 383 227 14 12 375 22 389 3.59e-38 146
MS.gene010312.t1 AT1G59750 31.694 366 219 13 12 358 22 375 1.51e-37 144
MS.gene010312.t1 AT1G34410 30.467 407 225 17 12 385 27 408 7.75e-37 141
MS.gene010312.t1 AT1G35540 31.311 412 215 21 12 385 27 408 7.29e-36 139
MS.gene010312.t1 AT1G34310 30.729 384 213 15 9 364 24 382 1.29e-35 138
MS.gene010312.t1 AT5G62000 28.818 347 210 10 12 335 61 393 5.87e-35 137
MS.gene010312.t1 AT5G62000 28.818 347 210 10 12 335 61 393 6.08e-35 137
MS.gene010312.t1 AT5G62000 28.818 347 210 10 12 335 61 393 6.08e-35 137
MS.gene010312.t1 AT5G62000 28.818 347 210 10 12 335 61 393 6.08e-35 137
MS.gene010312.t1 AT5G62000 28.818 347 210 10 12 335 61 393 6.08e-35 137
MS.gene010312.t1 AT4G23980 28.530 347 223 9 12 340 12 351 3.61e-34 134
MS.gene010312.t1 AT4G23980 28.530 347 223 9 12 340 12 351 3.67e-34 134
MS.gene010312.t1 AT1G35240 30.579 363 196 15 12 343 27 364 6.77e-34 133
MS.gene010312.t1 AT5G37020 30.409 342 213 12 12 335 22 356 3.19e-33 132
MS.gene010312.t1 AT1G30330 29.412 340 219 9 12 335 25 359 3.62e-33 132
MS.gene010312.t1 AT1G30330 29.412 340 219 9 12 335 25 359 3.62e-33 132
MS.gene010312.t1 AT1G30330 29.412 340 219 9 12 335 23 357 3.90e-33 132
MS.gene010312.t1 AT5G37020 30.409 342 213 12 12 335 22 356 4.20e-33 132
MS.gene010312.t1 AT1G34170 30.000 360 211 14 12 349 25 365 2.39e-32 128
MS.gene010312.t1 AT2G33860 29.609 358 215 12 12 347 54 396 1.31e-31 127
MS.gene010312.t1 AT1G19850 30.259 347 219 10 7 336 38 378 2.89e-31 126
MS.gene010312.t1 AT1G19850 30.259 347 219 10 7 336 49 389 3.61e-31 126
MS.gene010312.t1 AT1G19220 28.161 348 217 10 9 335 20 355 2.68e-30 124
MS.gene010312.t1 AT3G61830 30.272 294 188 6 59 342 24 310 5.16e-30 122
MS.gene010312.t1 AT5G20730 28.736 348 216 11 9 335 21 357 1.79e-29 121
MS.gene010312.t1 AT5G20730 28.736 348 216 11 9 335 21 357 1.80e-29 121
MS.gene010312.t1 AT5G20730 28.242 347 218 10 9 335 21 356 5.52e-29 120
MS.gene010312.t1 AT5G60450 28.775 351 213 13 12 335 65 405 4.06e-27 114
MS.gene010312.t1 AT5G60450 28.775 351 213 13 12 335 41 381 4.53e-27 114
MS.gene010312.t1 AT1G43950 32.353 204 118 6 12 203 27 222 3.19e-26 105
MS.gene010312.t1 AT1G34170 28.011 357 196 13 12 349 25 339 4.79e-26 109
MS.gene010312.t1 AT1G34170 28.011 357 196 13 12 349 25 339 8.95e-26 109
MS.gene010312.t1 AT2G46530 29.502 261 167 6 111 361 36 289 6.14e-24 103
MS.gene010312.t1 AT2G33860 29.435 248 153 9 112 347 2 239 1.83e-19 90.5
MS.gene010312.t1 AT5G37020 29.958 237 147 9 112 335 31 261 1.49e-17 85.1

Find 102 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGCATATCTGCCATATTT+TGG 0.143600 5.4:-47348039 None:intergenic
ACCAAGTGAGATATCTTTAT+TGG 0.193551 5.4:+47349653 MS.gene010312:CDS
AACTGATGAGATTAAGGTTT+TGG 0.252605 5.4:+47348667 MS.gene010312:CDS
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5.4:-47348080 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5.4:+47348915 MS.gene010312:CDS
TGTCTCCAACATCGTCTTCT+TGG 0.287447 5.4:-47348307 None:intergenic
GATGACAATTTCTCAGCTTA+AGG 0.289847 5.4:+47348868 MS.gene010312:CDS
GAATTTAACTGATGAGATTA+AGG 0.298587 5.4:+47348661 MS.gene010312:CDS
CGACCCTGCCAAGGTTTGTT+GGG 0.299743 5.4:-47349537 None:intergenic
TCACTTGGTGCTTGGCTCAT+TGG 0.299947 5.4:-47349639 None:intergenic
TGCCAAGGATTGACTAGGTT+TGG 0.313165 5.4:-47349419 None:intergenic
GATCTTTCCCAAGCTTGATT+TGG 0.314401 5.4:+47348409 MS.gene010312:CDS
ACCTCAAGCATGCGCCAATT+AGG 0.318991 5.4:-47348929 None:intergenic
TTGGCATATCTGTGCAATAT+TGG 0.331366 5.4:+47349672 MS.gene010312:CDS
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5.4:+47348797 MS.gene010312:CDS
CTACTTCGCACGAGGTCATC+TGG 0.355762 5.4:+47348103 MS.gene010312:CDS
TCGACCCTGCCAAGGTTTGT+TGG 0.360193 5.4:-47349538 None:intergenic
GAACCATTCTTGATCGTTAC+CGG 0.364840 5.4:+47348159 MS.gene010312:CDS
CCTCAAGCATGCGCCAATTA+GGG 0.367711 5.4:-47348928 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.375178 5.4:-47348065 None:intergenic
AGATTTCCAACCAATCTTCA+TGG 0.383662 5.4:-47348795 None:intergenic
TCCAACATCGTCTTCTTGGC+CGG 0.392440 5.4:-47348303 None:intergenic
ATGACAATTTCTCAGCTTAA+GGG 0.392754 5.4:+47348869 MS.gene010312:CDS
CTCGTGTGGAACCCAAAATA+TGG 0.402726 5.4:+47348027 MS.gene010312:CDS
GCCGGGAACTACAGGAGGAG+GGG 0.417463 5.4:-47348285 None:intergenic
TACGGGAGTCAGAAGCAGTT+TGG 0.422184 5.4:-47348249 None:intergenic
CGCTTCATCTGTAGTGAACT+TGG 0.424216 5.4:-47348624 None:intergenic
AATTCCGACAGATATCTTTC+CGG 0.425831 5.4:-47348591 None:intergenic
TGGCCGGGAACTACAGGAGG+AGG 0.428083 5.4:-47348287 None:intergenic
CGACCTTAAAAGCCGAGAAT+GGG 0.441124 5.4:+47348457 MS.gene010312:CDS
TGGAATCTTGACGTCGGGGC+CGG 0.443805 5.4:-47348060 None:intergenic
GACTCGTCCAAATCAAGCTT+GGG 0.456946 5.4:-47348416 None:intergenic
GTGTATTATCCGACAGCTAT+TGG 0.461034 5.4:+47348731 MS.gene010312:CDS
TCTTCTTGGCCGGGAACTAC+AGG 0.462528 5.4:-47348293 None:intergenic
CCGGGAACTACAGGAGGAGG+GGG 0.475602 5.4:-47348284 None:intergenic
GTTGACGATGCCATGAAGAT+TGG 0.477758 5.4:+47348785 MS.gene010312:CDS
GATGAAAACAACAGAATCGT+TGG 0.490573 5.4:-47348558 None:intergenic
AGACTCGTCCAAATCAAGCT+TGG 0.490804 5.4:-47348417 None:intergenic
GAAGATTCATATTTCTTCAA+AGG 0.492904 5.4:-47348836 None:intergenic
ATGGTTCTTGTGGCAGCAGT+AGG 0.493323 5.4:-47348143 None:intergenic
TTATCATAGGTTAACTGGGA+TGG 0.493859 5.4:+47349377 MS.gene010312:intron
CCGACCTTAAAAGCCGAGAA+TGG 0.494002 5.4:+47348456 MS.gene010312:CDS
GACCCTGCCAAGGTTTGTTG+GGG 0.496074 5.4:-47349536 None:intergenic
GGCGGAGTTGACGGTGGAGG+AGG 0.496620 5.4:-47349477 None:intergenic
TGAGGCGGAGTTGACGGTGG+AGG 0.507469 5.4:-47349480 None:intergenic
ATCCAAACCTAGTCAATCCT+TGG 0.508187 5.4:+47349417 MS.gene010312:CDS
CAACCTGCCAAGGATTGACT+AGG 0.513736 5.4:-47349424 None:intergenic
AAGATATCTCACTTGGTGCT+TGG 0.515083 5.4:-47349647 None:intergenic
TCATCCTCTGCCACCTCGTG+TGG 0.517268 5.4:+47348013 MS.gene010312:CDS
CCAACATCGTCTTCTTGGCC+GGG 0.517848 5.4:-47348302 None:intergenic
AATCTCAGTAGATTACATAC+TGG 0.519388 5.4:+47348500 MS.gene010312:CDS
CCCTAATTGGCGCATGCTTG+AGG 0.522896 5.4:+47348928 MS.gene010312:CDS
GGTTCCACACGAGGTGGCAG+AGG 0.527487 5.4:-47348017 None:intergenic
GAGACGATGCATGGAATGGA+TGG 0.527501 5.4:-47348182 None:intergenic
GGAAGAGACGATGCATGGAA+TGG 0.530637 5.4:-47348186 None:intergenic
TGGTTCTTGTGGCAGCAGTA+GGG 0.533159 5.4:-47348142 None:intergenic
TGACTAGGTTTGGATTCTGC+GGG 0.536979 5.4:-47349409 None:intergenic
GATGAACTCTCAGCAAGTCT+AGG 0.537540 5.4:-47349512 None:intergenic
GAGGAGGAAGTCGAAGGTGC+AGG 0.538875 5.4:-47349461 None:intergenic
AGATATCTGTCGGAATTCGC+AGG 0.543135 5.4:+47348597 MS.gene010312:CDS
ACTACAGATGAAGCGGATGA+AGG 0.543135 5.4:+47348632 MS.gene010312:CDS
CCCTGCCAAGGTTTGTTGGG+GGG 0.543970 5.4:-47349534 None:intergenic
TTTGGATGCAGTGGAATTGG+CGG 0.544683 5.4:+47348685 MS.gene010312:CDS
TTGTAAACTTCAACCTGCCA+AGG 0.544687 5.4:-47349434 None:intergenic
AAACCTAGTCAATCCTTGGC+AGG 0.546100 5.4:+47349421 MS.gene010312:CDS
GCAAAGACCTCGTCGGTGTG+TGG 0.555611 5.4:-47348227 None:intergenic
ACGATGCCATGAAGATTGGT+TGG 0.558168 5.4:+47348789 MS.gene010312:CDS
GCCAATAAAGATATCTCACT+TGG 0.558384 5.4:-47349654 None:intergenic
ACCCTGCCAAGGTTTGTTGG+GGG 0.559662 5.4:-47349535 None:intergenic
TCAGTAGATTACATACTGGC+TGG 0.560711 5.4:+47348504 MS.gene010312:CDS
CGATGCATGGAATGGATGGC+CGG 0.561229 5.4:-47348178 None:intergenic
GGTTCTTGTGGCAGCAGTAG+GGG 0.568653 5.4:-47348141 None:intergenic
ATTATCCGACAGCTATTGGT+TGG 0.574808 5.4:+47348735 MS.gene010312:CDS
GGCCGGGAACTACAGGAGGA+GGG 0.579671 5.4:-47348286 None:intergenic
TTGACTAGGTTTGGATTCTG+CGG 0.581555 5.4:-47349410 None:intergenic
TGGCCGGTAACGATCAAGAA+TGG 0.583189 5.4:-47348162 None:intergenic
CAAGTTCACTACAGATGAAG+CGG 0.584809 5.4:+47348625 MS.gene010312:CDS
AAAATATGGCAGATATGCGC+CGG 0.589425 5.4:+47348041 MS.gene010312:CDS
CCCGGCCAAGAAGACGATGT+TGG 0.598087 5.4:+47348302 MS.gene010312:CDS
TGACAATTTCTCAGCTTAAG+GGG 0.599563 5.4:+47348870 MS.gene010312:CDS
TAATCTACTGAGATTTGCAT+AGG 0.604127 5.4:-47348492 None:intergenic
TCAACGGAAGAGACGATGCA+TGG 0.604313 5.4:-47348191 None:intergenic
CAAACCTTGGCAGGGTCGAG+TGG 0.615213 5.4:+47349542 MS.gene010312:CDS
GACCTTAAAAGCCGAGAATG+GGG 0.616896 5.4:+47348458 MS.gene010312:CDS
ACAGATGAAGCGGATGAAGG+AGG 0.630115 5.4:+47348635 MS.gene010312:CDS
AATCTTTGGAATCTTGACGT+CGG 0.630358 5.4:-47348066 None:intergenic
ACGATCAAGAATGGTTCTTG+TGG 0.631537 5.4:-47348153 None:intergenic
TCTTGAGGCGGAGTTGACGG+TGG 0.632846 5.4:-47349483 None:intergenic
TCACGCCAACCAATAGCTGT+CGG 0.635734 5.4:-47348740 None:intergenic
AGTACCACTCGACCCTGCCA+AGG 0.642496 5.4:-47349546 None:intergenic
TCTGCCGGAAAGATATCTGT+CGG 0.642648 5.4:+47348587 MS.gene010312:CDS
TTGCGTGAGAGTCTTGACAT+AGG 0.647637 5.4:-47348339 None:intergenic
TGGATCAACAAAGAGATCAA+CGG 0.649094 5.4:-47348207 None:intergenic
ACCTTAAAAGCCGAGAATGG+GGG 0.652954 5.4:+47348459 MS.gene010312:CDS
TCTTGGCCGGGAACTACAGG+AGG 0.657068 5.4:-47348290 None:intergenic
AGGTGGCAGAGGATGAGACA+TGG 0.663852 5.4:-47348006 None:intergenic
TCTTTGGAATCTTGACGTCG+GGG 0.665271 5.4:-47348064 None:intergenic
CAGTTTGGCAAAGACCTCGT+CGG 0.665831 5.4:-47348234 None:intergenic
AAAGTTTACTACTTCGCACG+AGG 0.667496 5.4:+47348095 MS.gene010312:CDS
ATCAAGTTAGAGCATTCGAT+AGG 0.670484 5.4:-47348389 None:intergenic
TGTTGATCCACACACCGACG+AGG 0.698520 5.4:+47348220 MS.gene010312:CDS
ACGGTGGAGGAGGAAGTCGA+AGG 0.704506 5.4:-47349467 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTATCTTTTTATCAT+AGG + chr5.4:47349364-47349383 MS.gene010312:intron 15.0%
!! GAAATATTATTAATTCCCAT+TGG + chr5.4:47349108-47349127 MS.gene010312:intron 20.0%
!! TCAATCAAATAATCTTATCA+TGG - chr5.4:47348997-47349016 None:intergenic 20.0%
! AAAAGTTTCTACTTAGCATT+AGG - chr5.4:47349275-47349294 None:intergenic 25.0%
! GAAGATTCATATTTCTTCAA+AGG - chr5.4:47348839-47348858 None:intergenic 25.0%
! GAATTTAACTGATGAGATTA+AGG + chr5.4:47348661-47348680 MS.gene010312:CDS 25.0%
! GTACATATGCTTTCTTAATT+TGG + chr5.4:47348950-47348969 MS.gene010312:intron 25.0%
!! CTTTTTATCATAGGTTAACT+GGG + chr5.4:47349373-47349392 MS.gene010312:intron 25.0%
!! TCTTTTTATCATAGGTTAAC+TGG + chr5.4:47349372-47349391 MS.gene010312:intron 25.0%
!!! CTATTTTTAGTGAATATCAC+TGG - chr5.4:47349207-47349226 None:intergenic 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG - chr5.4:47348083-47348102 None:intergenic 25.0%
AATCTCAGTAGATTACATAC+TGG + chr5.4:47348500-47348519 MS.gene010312:CDS 30.0%
ACCAAGTGAGATATCTTTAT+TGG + chr5.4:47349653-47349672 MS.gene010312:CDS 30.0%
ATGACAATTTCTCAGCTTAA+GGG + chr5.4:47348869-47348888 MS.gene010312:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG + chr5.4:47348915-47348934 MS.gene010312:CDS 30.0%
TAATCTACTGAGATTTGCAT+AGG - chr5.4:47348495-47348514 None:intergenic 30.0%
! AACTGATGAGATTAAGGTTT+TGG + chr5.4:47348667-47348686 MS.gene010312:CDS 30.0%
!! AGGAGTATTTACAATGAGAA+AGG - chr5.4:47349255-47349274 None:intergenic 30.0%
AATTCCGACAGATATCTTTC+CGG - chr5.4:47348594-47348613 None:intergenic 35.0%
AGATTTCCAACCAATCTTCA+TGG - chr5.4:47348798-47348817 None:intergenic 35.0%
CGTTGCTTCATAACCATAAT+AGG + chr5.4:47349615-47349634 MS.gene010312:intron 35.0%
GATGAAAACAACAGAATCGT+TGG - chr5.4:47348561-47348580 None:intergenic 35.0%
GATGACAATTTCTCAGCTTA+AGG + chr5.4:47348868-47348887 MS.gene010312:CDS 35.0%
GCCAATAAAGATATCTCACT+TGG - chr5.4:47349657-47349676 None:intergenic 35.0%
TACAATGAGAAAGGCTTAGA+AGG - chr5.4:47349246-47349265 None:intergenic 35.0%
TGACAATTTCTCAGCTTAAG+GGG + chr5.4:47348870-47348889 MS.gene010312:CDS 35.0%
TGGATCAACAAAGAGATCAA+CGG - chr5.4:47348210-47348229 None:intergenic 35.0%
TTATCATAGGTTAACTGGGA+TGG + chr5.4:47349377-47349396 MS.gene010312:intron 35.0%
TTGGCATATCTGTGCAATAT+TGG + chr5.4:47349672-47349691 MS.gene010312:CDS 35.0%
! AAGTCTAGGAGTTTTTCTTG+AGG - chr5.4:47349501-47349520 None:intergenic 35.0%
! ATCAAGTTAGAGCATTCGAT+AGG - chr5.4:47348392-47348411 None:intergenic 35.0%
! ATGAAGATTGGTTGGAAATC+TGG + chr5.4:47348797-47348816 MS.gene010312:CDS 35.0%
! GTTTTCATCAAAAACTCTGC+CGG + chr5.4:47348572-47348591 MS.gene010312:CDS 35.0%
!! AATCTTTGGAATCTTGACGT+CGG - chr5.4:47348069-47348088 None:intergenic 35.0%
AAAATATGGCAGATATGCGC+CGG + chr5.4:47348041-47348060 MS.gene010312:CDS 40.0%
AAAGTTTACTACTTCGCACG+AGG + chr5.4:47348095-47348114 MS.gene010312:CDS 40.0%
ACGATCAAGAATGGTTCTTG+TGG - chr5.4:47348156-47348175 None:intergenic 40.0%
ATCCAAACCTAGTCAATCCT+TGG + chr5.4:47349417-47349436 MS.gene010312:CDS 40.0%
ATTATCCGACAGCTATTGGT+TGG + chr5.4:47348735-47348754 MS.gene010312:CDS 40.0%
CAAGTTCACTACAGATGAAG+CGG + chr5.4:47348625-47348644 MS.gene010312:CDS 40.0%
GAACCATTCTTGATCGTTAC+CGG + chr5.4:47348159-47348178 MS.gene010312:CDS 40.0%
GATCTTTCCCAAGCTTGATT+TGG + chr5.4:47348409-47348428 MS.gene010312:CDS 40.0%
GTGTATTATCCGACAGCTAT+TGG + chr5.4:47348731-47348750 MS.gene010312:CDS 40.0%
TATATGTGTACCCCCATTCT+CGG - chr5.4:47348472-47348491 None:intergenic 40.0%
TCAGTAGATTACATACTGGC+TGG + chr5.4:47348504-47348523 MS.gene010312:CDS 40.0%
TTGACTAGGTTTGGATTCTG+CGG - chr5.4:47349413-47349432 None:intergenic 40.0%
TTGGCTCATTGGACCTATTA+TGG - chr5.4:47349631-47349650 None:intergenic 40.0%
TTGTAAACTTCAACCTGCCA+AGG - chr5.4:47349437-47349456 None:intergenic 40.0%
! GCGCATATCTGCCATATTTT+GGG - chr5.4:47348041-47348060 None:intergenic 40.0%
! TCTAGGAGTTTTTCTTGAGG+CGG - chr5.4:47349498-47349517 None:intergenic 40.0%
!! AAGATATCTCACTTGGTGCT+TGG - chr5.4:47349650-47349669 None:intergenic 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG - chr5.4:47348068-47348087 None:intergenic 40.0%
!!! GATTAAGGTTTTGGATGCAG+TGG + chr5.4:47348676-47348695 MS.gene010312:CDS 40.0%
AAACCTAGTCAATCCTTGGC+AGG + chr5.4:47349421-47349440 MS.gene010312:CDS 45.0%
ACCTTAAAAGCCGAGAATGG+GGG + chr5.4:47348459-47348478 MS.gene010312:CDS 45.0%
ACGATGCCATGAAGATTGGT+TGG + chr5.4:47348789-47348808 MS.gene010312:CDS 45.0%
ACTACAGATGAAGCGGATGA+AGG + chr5.4:47348632-47348651 MS.gene010312:CDS 45.0%
AGACTCGTCCAAATCAAGCT+TGG - chr5.4:47348420-47348439 None:intergenic 45.0%
AGATATCTGTCGGAATTCGC+AGG + chr5.4:47348597-47348616 MS.gene010312:CDS 45.0%
CCAAACCTTGGCAGTCTTAA+CGG + chr5.4:47349584-47349603 MS.gene010312:intron 45.0%
CGACCTTAAAAGCCGAGAAT+GGG + chr5.4:47348457-47348476 MS.gene010312:CDS 45.0%
CGCTTCATCTGTAGTGAACT+TGG - chr5.4:47348627-47348646 None:intergenic 45.0%
CTATCGAGTGCAAGACCAAT+GGG - chr5.4:47349126-47349145 None:intergenic 45.0%
CTCGTGTGGAACCCAAAATA+TGG + chr5.4:47348027-47348046 MS.gene010312:CDS 45.0%
GACCTTAAAAGCCGAGAATG+GGG + chr5.4:47348458-47348477 MS.gene010312:CDS 45.0%
GACTCGTCCAAATCAAGCTT+GGG - chr5.4:47348419-47348438 None:intergenic 45.0%
GATGAACTCTCAGCAAGTCT+AGG - chr5.4:47349515-47349534 None:intergenic 45.0%
GTTGACGATGCCATGAAGAT+TGG + chr5.4:47348785-47348804 MS.gene010312:CDS 45.0%
TCTATCGAGTGCAAGACCAA+TGG - chr5.4:47349127-47349146 None:intergenic 45.0%
TCTGCCGGAAAGATATCTGT+CGG + chr5.4:47348587-47348606 MS.gene010312:CDS 45.0%
TGACTAGGTTTGGATTCTGC+GGG - chr5.4:47349412-47349431 None:intergenic 45.0%
TGCCAAGGATTGACTAGGTT+TGG - chr5.4:47349422-47349441 None:intergenic 45.0%
TGTCTCCAACATCGTCTTCT+TGG - chr5.4:47348310-47348329 None:intergenic 45.0%
TTGCGTGAGAGTCTTGACAT+AGG - chr5.4:47348342-47348361 None:intergenic 45.0%
TTTGGATGCAGTGGAATTGG+CGG + chr5.4:47348685-47348704 MS.gene010312:CDS 45.0%
! GGCGCATATCTGCCATATTT+TGG - chr5.4:47348042-47348061 None:intergenic 45.0%
! TTTTCTTGAGGCGGAGTTGA+CGG - chr5.4:47349489-47349508 None:intergenic 45.0%
!! TCTTTGGAATCTTGACGTCG+GGG - chr5.4:47348067-47348086 None:intergenic 45.0%
!!! CATATTTTGGGTTCCACACG+AGG - chr5.4:47348029-47348048 None:intergenic 45.0%
!!! CCATTCTCGGCTTTTAAGGT+CGG - chr5.4:47348459-47348478 None:intergenic 45.0%
!!! GGTTTTGGATGCAGTGGAAT+TGG + chr5.4:47348682-47348701 MS.gene010312:CDS 45.0%
ACAGATGAAGCGGATGAAGG+AGG + chr5.4:47348635-47348654 MS.gene010312:CDS 50.0%
ACCTCAAGCATGCGCCAATT+AGG - chr5.4:47348932-47348951 None:intergenic 50.0%
AGGGGGTTCTTGGTCAGTTA+CGG - chr5.4:47348270-47348289 None:intergenic 50.0%
ATGGTTCTTGTGGCAGCAGT+AGG - chr5.4:47348146-47348165 None:intergenic 50.0%
CAACCTGCCAAGGATTGACT+AGG - chr5.4:47349427-47349446 None:intergenic 50.0%
CAGTTTGGCAAAGACCTCGT+CGG - chr5.4:47348237-47348256 None:intergenic 50.0%
CCAAGGTTTGGCTATGTCTC+AGG - chr5.4:47349575-47349594 None:intergenic 50.0%
CCGACCTTAAAAGCCGAGAA+TGG + chr5.4:47348456-47348475 MS.gene010312:CDS 50.0%
CCGTTAAGACTGCCAAGGTT+TGG - chr5.4:47349587-47349606 None:intergenic 50.0%
CCTCAAGCATGCGCCAATTA+GGG - chr5.4:47348931-47348950 None:intergenic 50.0%
CCTGAGACATAGCCAAACCT+TGG + chr5.4:47349572-47349591 MS.gene010312:intron 50.0%
GAGACGATGCATGGAATGGA+TGG - chr5.4:47348185-47348204 None:intergenic 50.0%
GGAAGAGACGATGCATGGAA+TGG - chr5.4:47348189-47348208 None:intergenic 50.0%
TACGGGAGTCAGAAGCAGTT+TGG - chr5.4:47348252-47348271 None:intergenic 50.0%
TCAACGGAAGAGACGATGCA+TGG - chr5.4:47348194-47348213 None:intergenic 50.0%
TCACGCCAACCAATAGCTGT+CGG - chr5.4:47348743-47348762 None:intergenic 50.0%
TCCAACATCGTCTTCTTGGC+CGG - chr5.4:47348306-47348325 None:intergenic 50.0%
TGGCCGGTAACGATCAAGAA+TGG - chr5.4:47348165-47348184 None:intergenic 50.0%
TGGTTCTTGTGGCAGCAGTA+GGG - chr5.4:47348145-47348164 None:intergenic 50.0%
TTCATCCCCCCAACAAACCT+TGG + chr5.4:47349529-47349548 MS.gene010312:CDS 50.0%
! ACCCCCATTCTCGGCTTTTA+AGG - chr5.4:47348463-47348482 None:intergenic 50.0%
! TGGTTGGCGTGATTTTGTCG+TGG + chr5.4:47348751-47348770 MS.gene010312:CDS 50.0%
!! TCACTTGGTGCTTGGCTCAT+TGG - chr5.4:47349642-47349661 None:intergenic 50.0%
!!! ATTTTGGGTTCCACACGAGG+TGG - chr5.4:47348026-47348045 None:intergenic 50.0%
CAGCACCGTTAAGACTGCCA+AGG - chr5.4:47349592-47349611 None:intergenic 55.0%
CCAACATCGTCTTCTTGGCC+GGG - chr5.4:47348305-47348324 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTG+AGG + chr5.4:47348928-47348947 MS.gene010312:CDS 55.0%
CGATGCATGGAATGGATGGC+CGG - chr5.4:47348181-47348200 None:intergenic 55.0%
CTACTTCGCACGAGGTCATC+TGG + chr5.4:47348103-47348122 MS.gene010312:CDS 55.0%
GGGGGTTCTTGGTCAGTTAC+GGG - chr5.4:47348269-47348288 None:intergenic 55.0%
GGTTCTTGTGGCAGCAGTAG+GGG - chr5.4:47348144-47348163 None:intergenic 55.0%
TCGACCCTGCCAAGGTTTGT+TGG - chr5.4:47349541-47349560 None:intergenic 55.0%
TCTTCTTGGCCGGGAACTAC+AGG - chr5.4:47348296-47348315 None:intergenic 55.0%
TGTTGATCCACACACCGACG+AGG + chr5.4:47348220-47348239 MS.gene010312:CDS 55.0%
! CGACCCTGCCAAGGTTTGTT+GGG - chr5.4:47349540-47349559 None:intergenic 55.0%
!! ACCCTGCCAAGGTTTGTTGG+GGG - chr5.4:47349538-47349557 None:intergenic 55.0%
!! GACCCTGCCAAGGTTTGTTG+GGG - chr5.4:47349539-47349558 None:intergenic 55.0%
ACGGTGGAGGAGGAAGTCGA+AGG - chr5.4:47349470-47349489 None:intergenic 60.0%
AGTACCACTCGACCCTGCCA+AGG - chr5.4:47349549-47349568 None:intergenic 60.0%
CAAACCTTGGCAGGGTCGAG+TGG + chr5.4:47349542-47349561 MS.gene010312:CDS 60.0%
CCCCCCAACAAACCTTGGCA+GGG + chr5.4:47349534-47349553 MS.gene010312:CDS 60.0%
CCCGGCCAAGAAGACGATGT+TGG + chr5.4:47348302-47348321 MS.gene010312:CDS 60.0%
CTACAGGAGGAGGGGGTTCT+TGG - chr5.4:47348280-47348299 None:intergenic 60.0%
GCAAAGACCTCGTCGGTGTG+TGG - chr5.4:47348230-47348249 None:intergenic 60.0%
TCATCCTCTGCCACCTCGTG+TGG + chr5.4:47348013-47348032 MS.gene010312:CDS 60.0%
TCCCCCCAACAAACCTTGGC+AGG + chr5.4:47349533-47349552 MS.gene010312:CDS 60.0%
TCTTGAGGCGGAGTTGACGG+TGG - chr5.4:47349486-47349505 None:intergenic 60.0%
TCTTGGCCGGGAACTACAGG+AGG - chr5.4:47348293-47348312 None:intergenic 60.0%
TGGAATCTTGACGTCGGGGC+CGG - chr5.4:47348063-47348082 None:intergenic 60.0%
! GAGGAGGAAGTCGAAGGTGC+AGG - chr5.4:47349464-47349483 None:intergenic 60.0%
!! CCCTGCCAAGGTTTGTTGGG+GGG - chr5.4:47349537-47349556 None:intergenic 60.0%
CCCCCTCCTCCTGTAGTTCC+CGG + chr5.4:47348284-47348303 MS.gene010312:CDS 65.0%
CCGGGAACTACAGGAGGAGG+GGG - chr5.4:47348287-47348306 None:intergenic 65.0%
GCCGGGAACTACAGGAGGAG+GGG - chr5.4:47348288-47348307 None:intergenic 65.0%
GGCCGGGAACTACAGGAGGA+GGG - chr5.4:47348289-47348308 None:intergenic 65.0%
GGTTCCACACGAGGTGGCAG+AGG - chr5.4:47348020-47348039 None:intergenic 65.0%
TGAGGCGGAGTTGACGGTGG+AGG - chr5.4:47349483-47349502 None:intergenic 65.0%
TGGCCGGGAACTACAGGAGG+AGG - chr5.4:47348290-47348309 None:intergenic 65.0%
GGCGGAGTTGACGGTGGAGG+AGG - chr5.4:47349480-47349499 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 47348008 47349722 47348008 ID=MS.gene010312
chr5.4 mRNA 47348008 47349722 47348008 ID=MS.gene010312.t1;Parent=MS.gene010312
chr5.4 exon 47348008 47348949 47348008 ID=MS.gene010312.t1.exon1;Parent=MS.gene010312.t1
chr5.4 CDS 47348008 47348949 47348008 ID=cds.MS.gene010312.t1;Parent=MS.gene010312.t1
chr5.4 exon 47349386 47349563 47349386 ID=MS.gene010312.t1.exon2;Parent=MS.gene010312.t1
chr5.4 CDS 47349386 47349563 47349386 ID=cds.MS.gene010312.t1;Parent=MS.gene010312.t1
chr5.4 exon 47349637 47349722 47349637 ID=MS.gene010312.t1.exon3;Parent=MS.gene010312.t1
chr5.4 CDS 47349637 47349722 47349637 ID=cds.MS.gene010312.t1;Parent=MS.gene010312.t1
Gene Sequence

>MS.gene010312

ATGTCTCATCCTCTGCCACCTCGTGTGGAACCCAAAATATGGCAGATATGCGCCGGCCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCGCACGAGGTCATCTGGAACATGCATGTTCTTCCCCTACTGCTGCCACAAGAACCATTCTTGATCGTTACCGGCCATCCATTCCATGCATCGTCTCTTCCGTTGATCTCTTTGTTGATCCACACACCGACGAGGTCTTTGCCAAACTGCTTCTGACTCCCGTAACTGACCAAGAACCCCCTCCTCCTGTAGTTCCCGGCCAAGAAGACGATGTTGGAGACAATCTTGTTTCCTATGTCAAGACTCTCACGCAATCTGATTGTGCAAGAGTATTATGTGTACCTATCGAATGCTCTAACTTGATCTTTCCCAAGCTTGATTTGGACGAGTCTCAGAGCATCACTGTCACCGACCTTAAAAGCCGAGAATGGGGGTACACATATACCTATGCAAATCTCAGTAGATTACATACTGGCTGGTTAAATTTTGTACGAGAGAAGAAACTCGTCGCCAACGATTCTGTTGTTTTCATCAAAAACTCTGCCGGAAAGATATCTGTCGGAATTCGCAGGAACACCAAGTTCACTACAGATGAAGCGGATGAAGGAGGCGAGAATTTAACTGATGAGATTAAGGTTTTGGATGCAGTGGAATTGGCGGAGAAGAACACAGCATTTGATGTTGTGTATTATCCGACAGCTATTGGTTGGCGTGATTTTGTCGTGGATGCTAAGACTGTTGACGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGAGTGAAACTTCCTTTGAAGAAATATGAATCTTCAAGTTCAAAGATGACAATTTCTCAGCTTAAGGGGACAATCTCTTTTGTCTTTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTGAGGTACATATGCTTTCTTAATTTGGTCTGATTATATTTAGTTGAATCCATGATAAGATTATTTGATTGATATATATGAGTGTTGTTGAAGTTAATTCATGAAACTATAATTAGATGATACTTTATGATACAGAAAAATGTAACAGAATCAGATTACACTTGAAATATTATTAATTCCCATTGGTCTTGCACTCGATAGATATCTCATGACACTTAATGTTTACTTTATGTAATCAGATTGTTTTTATACGAAACTGCCAGTGATATTCACTAAAAATAGTTATGAAGTTTAAGAACCTTCTAAGCCTTTCTCATTGTAAATACTCCTAATGCTAAGTAGAAACTTTTGATGTCTAAATACATATATACTATATATATTTTATCAAATATTTCAAATTATTGATATTGATTCTTACTGTTTTTTATCTTTTTATCATAGGTTAACTGGGATGGACTTGATTTCCCGCAGAATCCAAACCTAGTCAATCCTTGGCAGGTTGAAGTTTACAACATACCTGCACCTTCGACTTCCTCCTCCACCGTCAACTCCGCCTCAAGAAAAACTCCTAGACTTGCTGAGAGTTCATCCCCCCAACAAACCTTGGCAGGGTCGAGTGGTACTTTGCCTGAGACATAGCCAAACCTTGGCAGTCTTAACGGTGCTGACTCGTTGCTTCATAACCATAATAGGTCCAATGAGCCAAGCACCAAGTGAGATATCTTTATTGGCATATCTGTGCAATATTGGTATGCAGTCACAATTAGAAACTCAGTGA
Protein sequence

>MS.gene010312.t1

MSHPLPPRVEPKIWQICAGPDVKIPKIHSKVYYFARGHLEHACSSPTAATRTILDRYRPSIPCIVSSVDLFVDPHTDEVFAKLLLTPVTDQEPPPPVVPGQEDDVGDNLVSYVKTLTQSDCARVLCVPIECSNLIFPKLDLDESQSITVTDLKSREWGYTYTYANLSRLHTGWLNFVREKKLVANDSVVFIKNSAGKISVGIRRNTKFTTDEADEGGENLTDEIKVLDAVELAEKNTAFDVVYYPTAIGWRDFVVDAKTVDDAMKIGWKSGMRVKLPLKKYESSSSKMTISQLKGTISFVFNDSSNVPNWRMLEVNWDGLDFPQNPNLVNPWQVEVYNIPAPSTSSSTVNSASRKTPRLAESSSPQQTLAGSSGPMSQAPSEISLLAYLCNIGMQSQLETQ