AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010313

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010313.t1 MTR_5g061890 61.168 394 121 10 1 379 1 377 4.18e-149 427
MS.gene010313.t1 MTR_5g060780 60.406 394 124 10 1 379 1 377 2.73e-147 422
MS.gene010313.t1 MTR_5g460920 60.152 394 125 10 1 379 1 377 2.79e-145 417
MS.gene010313.t1 MTR_5g060630 52.983 419 139 9 1 365 1 415 2.28e-132 389
MS.gene010313.t1 MTR_5g460740 52.983 419 139 9 1 365 1 415 2.28e-132 389
MS.gene010313.t1 MTR_5g061220 55.721 402 120 12 1 361 8 392 3.41e-132 389
MS.gene010313.t1 MTR_1g058210 56.499 377 128 10 4 361 3 362 7.54e-128 375
MS.gene010313.t1 MTR_2g024430 52.000 375 143 16 4 361 11 365 1.08e-107 326
MS.gene010313.t1 MTR_5g040880 51.282 351 143 11 3 338 1 338 2.03e-102 312
MS.gene010313.t1 MTR_5g082140 48.588 354 150 11 4 338 3 343 8.28e-100 304
MS.gene010313.t1 MTR_2g006270 47.000 400 177 14 1 374 1 391 8.10e-98 298
MS.gene010313.t1 MTR_5g040740 50.617 324 133 11 4 312 3 314 1.39e-89 276
MS.gene010313.t1 MTR_2g006380 44.416 394 177 16 7 374 14 391 7.52e-89 275
MS.gene010313.t1 MTR_3g073420 40.731 383 168 10 3 333 6 381 9.03e-74 240
MS.gene010313.t1 MTR_1g064430 36.516 419 189 13 8 364 22 425 1.68e-69 231
MS.gene010313.t1 MTR_1g064430 36.754 419 188 14 8 364 35 438 1.93e-69 231
MS.gene010313.t1 MTR_1g094960 37.722 395 161 14 8 337 14 388 9.28e-67 222
MS.gene010313.t1 MTR_5g062970 55.288 208 81 7 13 214 38 239 9.63e-67 211
MS.gene010313.t1 MTR_4g058930 38.083 386 169 12 13 337 12 388 9.50e-66 221
MS.gene010313.t1 MTR_4g058930 38.083 386 169 12 13 337 12 388 1.92e-65 220
MS.gene010313.t1 MTR_2g094570 37.467 379 173 13 13 336 11 380 8.82e-65 218
MS.gene010313.t1 MTR_2g094570 37.467 379 173 13 13 336 11 380 8.85e-65 219
MS.gene010313.t1 MTR_2g094570 37.467 379 173 13 13 336 67 436 2.15e-64 219
MS.gene010313.t1 MTR_7g101280 34.264 394 190 10 8 338 26 413 2.47e-63 214
MS.gene010313.t1 MTR_0041s0110 34.264 394 190 10 8 338 26 413 2.47e-63 214
MS.gene010313.t1 MTR_5g060770 44.089 313 102 9 65 368 9 257 9.30e-63 205
MS.gene010313.t1 MTR_7g101275 33.875 369 205 13 23 366 2 356 1.67e-53 182
MS.gene010313.t1 MTR_2g014770 30.184 381 225 10 3 352 44 414 3.93e-48 172
MS.gene010313.t1 MTR_2g014770 30.709 381 223 11 3 352 44 414 8.13e-48 172
MS.gene010313.t1 MTR_4g021580 33.791 364 207 12 13 355 19 369 2.34e-47 170
MS.gene010313.t1 MTR_0041s0120 31.746 378 213 12 23 366 2 368 3.26e-47 166
MS.gene010313.t1 MTR_4g021580 33.791 364 207 12 13 355 19 369 3.52e-47 170
MS.gene010313.t1 MTR_2g005240 33.903 351 200 11 13 340 23 364 2.74e-46 168
MS.gene010313.t1 MTR_4g088210 30.105 382 224 11 13 366 38 404 7.42e-44 161
MS.gene010313.t1 MTR_2g093740 30.000 400 227 14 13 376 56 438 8.06e-44 161
MS.gene010313.t1 MTR_4g088210 30.105 382 224 11 13 366 38 404 8.27e-44 161
MS.gene010313.t1 MTR_2g093740 30.000 400 227 14 13 376 56 438 1.44e-43 161
MS.gene010313.t1 MTR_2g093740 30.000 400 227 14 13 376 56 438 1.51e-43 161
MS.gene010313.t1 MTR_2g018690 31.933 357 211 11 1 332 8 357 2.05e-43 161
MS.gene010313.t1 MTR_8g100050 32.659 346 204 11 13 337 59 396 2.06e-43 160
MS.gene010313.t1 MTR_8g100050 32.659 346 204 11 13 337 59 396 5.10e-43 160
MS.gene010313.t1 MTR_4g124900 31.339 351 202 11 8 332 18 355 1.56e-42 159
MS.gene010313.t1 MTR_4g124900 31.339 351 202 11 8 332 18 355 1.62e-42 159
MS.gene010313.t1 MTR_2g093740 29.056 413 227 14 13 376 56 451 5.61e-42 156
MS.gene010313.t1 MTR_8g079492 29.805 359 215 9 1 332 8 356 6.02e-42 157
MS.gene010313.t1 MTR_8g079492 29.805 359 215 9 1 332 8 356 6.49e-42 157
MS.gene010313.t1 MTR_2g093740 29.056 413 227 14 13 376 56 451 6.74e-42 156
MS.gene010313.t1 MTR_2g093740 29.056 413 227 14 13 376 56 451 1.06e-41 156
MS.gene010313.t1 MTR_7g062540 30.939 362 223 11 13 356 14 366 2.02e-41 154
MS.gene010313.t1 MTR_4g060460 29.695 394 234 10 13 374 54 436 2.46e-41 155
MS.gene010313.t1 MTR_5g460910 35.583 326 115 8 52 368 13 252 4.39e-41 147
MS.gene010313.t1 MTR_1g024025 30.925 346 213 10 8 333 38 377 4.79e-41 154
MS.gene010313.t1 MTR_2g043250 29.915 351 207 11 8 332 18 355 6.45e-41 154
MS.gene010313.t1 MTR_4g060460 29.815 379 225 9 13 359 54 423 1.48e-39 150
MS.gene010313.t1 MTR_3g064050 29.201 363 196 10 8 332 21 360 1.84e-39 150
MS.gene010313.t1 MTR_5g076270 28.933 356 210 10 6 332 17 358 1.34e-38 147
MS.gene010313.t1 MTR_8g027440 32.133 361 215 12 13 355 29 377 1.53e-38 146
MS.gene010313.t1 MTR_8g101360 29.944 354 201 10 10 332 28 365 1.92e-37 144
MS.gene010313.t1 MTR_5g062960 54.955 111 40 1 235 335 1 111 1.21e-30 116
MS.gene010313.t1 MTR_8g079492 29.682 283 168 7 72 332 1 274 1.64e-30 123
MS.gene010313.t1 MTR_2g093740 29.433 282 166 9 117 376 38 308 1.57e-26 111
MS.gene010313.t1 MTR_2g093740 29.433 282 166 9 117 376 38 308 1.70e-26 111
MS.gene010313.t1 MTR_2g093740 28.136 295 166 9 117 376 38 321 7.29e-25 106
MS.gene010313.t1 MTR_2g093740 28.136 295 166 9 117 376 38 321 8.35e-25 106
MS.gene010313.t1 MTR_8g446900 25.000 288 191 8 61 332 10 288 2.29e-21 94.7
MS.gene010313.t1 MTR_5g046120 46.465 99 49 4 66 161 26 123 7.95e-16 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010313.t1 AT1G77850 39.175 388 153 12 8 334 14 379 2.64e-76 247
MS.gene010313.t1 AT1G77850 39.175 388 153 12 8 334 14 379 3.30e-76 247
MS.gene010313.t1 AT4G30080 39.950 398 154 15 13 344 19 397 1.20e-70 234
MS.gene010313.t1 AT2G28350 37.280 397 172 15 6 337 3 387 7.47e-64 216
MS.gene010313.t1 AT2G28350 37.280 397 172 15 6 337 3 387 8.83e-64 216
MS.gene010313.t1 AT1G34390 34.561 353 189 12 13 340 27 362 2.32e-51 181
MS.gene010313.t1 AT2G33860 31.746 378 220 11 13 365 54 418 7.44e-49 174
MS.gene010313.t1 AT4G23980 33.333 336 203 9 13 332 12 342 2.74e-48 173
MS.gene010313.t1 AT4G23980 33.333 336 203 9 13 332 12 342 3.05e-48 173
MS.gene010313.t1 AT2G46530 33.333 348 186 12 13 332 21 350 3.15e-48 172
MS.gene010313.t1 AT1G34310 35.072 345 186 12 10 332 24 352 3.54e-48 172
MS.gene010313.t1 AT2G46530 33.333 348 186 12 13 332 42 371 4.39e-48 172
MS.gene010313.t1 AT1G35520 34.503 342 186 12 13 332 27 352 2.39e-46 167
MS.gene010313.t1 AT1G35240 33.616 354 195 13 13 342 27 364 9.31e-45 163
MS.gene010313.t1 AT3G61830 31.458 391 228 13 13 376 25 402 2.63e-44 162
MS.gene010313.t1 AT5G62000 32.174 345 207 10 13 337 61 398 4.23e-44 163
MS.gene010313.t1 AT5G62000 32.174 345 207 10 13 337 61 398 4.92e-44 163
MS.gene010313.t1 AT5G62000 32.174 345 207 10 13 337 61 398 4.92e-44 163
MS.gene010313.t1 AT5G62000 32.174 345 207 10 13 337 61 398 4.92e-44 163
MS.gene010313.t1 AT5G62000 32.174 345 207 10 13 337 61 398 4.92e-44 163
MS.gene010313.t1 AT1G35540 32.749 342 192 11 13 332 27 352 1.40e-43 160
MS.gene010313.t1 AT1G59750 32.153 339 204 10 13 332 22 353 2.28e-43 160
MS.gene010313.t1 AT1G59750 32.153 339 204 10 13 332 22 353 2.29e-43 160
MS.gene010313.t1 AT1G59750 32.153 339 204 10 13 332 22 353 2.59e-43 160
MS.gene010313.t1 AT1G59750 32.153 339 204 10 13 332 22 353 2.59e-43 160
MS.gene010313.t1 AT1G34410 32.880 368 204 14 13 358 27 373 4.49e-43 158
MS.gene010313.t1 AT5G37020 29.213 356 205 11 8 332 17 356 6.24e-42 157
MS.gene010313.t1 AT5G37020 29.213 356 205 11 8 332 17 356 9.27e-42 156
MS.gene010313.t1 AT1G30330 31.742 356 207 12 5 332 10 357 1.42e-41 156
MS.gene010313.t1 AT1G30330 31.609 348 207 11 8 332 20 359 2.56e-41 155
MS.gene010313.t1 AT1G30330 31.609 348 207 11 8 332 20 359 2.56e-41 155
MS.gene010313.t1 AT5G20730 31.143 350 204 11 8 332 19 356 6.42e-41 154
MS.gene010313.t1 AT1G19220 29.310 348 209 10 10 332 20 355 8.07e-40 151
MS.gene010313.t1 AT5G20730 31.161 353 201 13 8 332 19 357 9.07e-40 151
MS.gene010313.t1 AT5G20730 31.161 353 201 13 8 332 19 357 1.02e-39 150
MS.gene010313.t1 AT5G60450 29.474 380 215 14 10 355 38 398 3.94e-39 149
MS.gene010313.t1 AT5G60450 29.474 380 215 14 10 355 62 422 5.59e-39 148
MS.gene010313.t1 AT1G19850 30.925 346 212 11 9 333 50 389 1.26e-38 147
MS.gene010313.t1 AT1G19850 30.925 346 212 11 9 333 39 378 1.72e-38 147
MS.gene010313.t1 AT1G34170 30.028 353 216 12 13 346 25 365 4.87e-36 137
MS.gene010313.t1 AT3G61830 32.281 285 169 9 64 332 27 303 1.47e-34 134
MS.gene010313.t1 AT2G46530 34.800 250 132 8 100 332 28 263 1.11e-33 131
MS.gene010313.t1 AT2G33860 31.461 267 158 8 117 365 2 261 2.74e-32 126
MS.gene010313.t1 AT1G34170 28.448 348 202 11 13 346 25 339 1.61e-31 124
MS.gene010313.t1 AT1G34170 28.448 348 202 11 13 346 25 339 3.41e-31 124
MS.gene010313.t1 AT1G43950 36.150 213 106 8 13 212 27 222 1.66e-29 114
MS.gene010313.t1 AT5G37020 28.309 272 160 9 85 332 1 261 2.04e-26 111

Find 114 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATTGGATTGTTAAAATT+TGG 0.112967 5.4:-47312657 None:intergenic
ATGAAGATTGTTTGGAATTC+TGG 0.127863 5.4:+47313044 MS.gene010313:CDS
CTTCTTCGAATTCCCCTAAT+TGG 0.184271 5.4:+47313132 MS.gene010313:CDS
GTTAACCGAGTCGGCGGTTT+TGG 0.206731 5.4:+47312917 MS.gene010313:CDS
GCATGTTCCAAGTGACCTTT+TGG 0.275383 5.4:-47312339 None:intergenic
AGGAGGGTGAAGAGGGAGGC+TGG 0.281049 5.4:+47312883 MS.gene010313:CDS
GTGAAGAGGGAGGCTGGAAG+AGG 0.299344 5.4:+47312889 MS.gene010313:CDS
CCTGAAGAATGTTCCAATTA+GGG 0.299732 5.4:-47313145 None:intergenic
CCACAGGAGGAGCTGGTTCT+TGG 0.300062 5.4:-47312527 None:intergenic
ACCTGAAGAATGTTCCAATT+AGG 0.304192 5.4:-47313146 None:intergenic
GCGTTGTCGAAGGCAAAATT+TGG 0.307258 5.4:+47312256 MS.gene010313:CDS
TGCCAAGGATTGACTAGGTT+TGG 0.313165 5.4:-47313807 None:intergenic
TGTATTATCCAAGTCATAAT+TGG 0.331214 5.4:+47312982 MS.gene010313:CDS
CCCTAATTGGAACATTCTTC+AGG 0.342494 5.4:+47313145 MS.gene010313:CDS
TTTGGATTCTGCGGGATATT+AGG 0.363066 5.4:-47313789 None:intergenic
CAAATTCTACATTCAATTGC+AGG 0.366385 5.4:+47313930 MS.gene010313:CDS
TGGAATCTTGACGCCGGGGC+CGG 0.386761 5.4:-47312289 None:intergenic
ATGACGCTATGAAGATTGTT+TGG 0.387773 5.4:+47313036 MS.gene010313:CDS
GTAGAGAAACGGTGTCTATC+AGG 0.389463 5.4:-47312750 None:intergenic
CCGATGTTCAAGGCCAAGTC+TGG 0.398680 5.4:+47312700 MS.gene010313:CDS
TGAAGAGGGAGGCTGGAAGA+GGG 0.400454 5.4:+47312890 MS.gene010313:CDS
TAAACGACTGAGTTCAGCTT+TGG 0.405710 5.4:-47312309 None:intergenic
TGAAGATTGTTTGGAATTCT+GGG 0.411575 5.4:+47313045 MS.gene010313:CDS
AATTGAATGTAGAATTTGTT+GGG 0.424854 5.4:-47313925 None:intergenic
TAACAATCCAATGCCGTCTC+AGG 0.429929 5.4:+47312665 MS.gene010313:CDS
GGTGAGATTGACAGTGGAGG+AGG 0.429947 5.4:-47313865 None:intergenic
CGCTGAGATGATGCAGAGAT+AGG 0.432301 5.4:-47312418 None:intergenic
TGGCAGACATGCGCCGGCCC+CGG 0.432533 5.4:+47312276 MS.gene010313:CDS
AGCTTTGGAATCTTGACGCC+GGG 0.433725 5.4:-47312294 None:intergenic
TACCGGAGTGAGGATGAGTT+TGG 0.435570 5.4:-47312481 None:intergenic
AAGGAGACTGTCTGAGGTAG+TGG 0.438861 5.4:-47312372 None:intergenic
CCAAGGTTTGGCTACGCGTC+AGG 0.440106 5.4:-47313969 None:intergenic
CACCCTCCTTCTTTATGTGC+CGG 0.454754 5.4:-47312869 None:intergenic
CGTTTCTCTACTGGCTGGAC+TGG 0.455390 5.4:+47312762 MS.gene010313:CDS
CATAAAGAAGGAGGGTGAAG+AGG 0.457435 5.4:+47312875 MS.gene010313:CDS
GATAGACACCGTTTCTCTAC+TGG 0.461405 5.4:+47312753 MS.gene010313:CDS
CAATTGAATGTAGAATTTGT+TGG 0.464823 5.4:-47313926 None:intergenic
TTATCATAGGTTAACTGGGA+TGG 0.471984 5.4:+47313765 MS.gene010313:intron
GAGGAGCTGGTTCTTGGACT+TGG 0.482484 5.4:-47312521 None:intergenic
ACACCACCGTCAATTGTTAC+CGG 0.486756 5.4:-47312498 None:intergenic
CCTTCGACAACGCGTGGCTG+AGG 0.488355 5.4:-47312246 None:intergenic
TTTGAAGAATGATCAGTCCC+TGG 0.489333 5.4:+47313085 MS.gene010313:CDS
CAATTAGGGGAATTCGAAGA+AGG 0.495700 5.4:-47313131 None:intergenic
AGAATGAAGATCATGAAGAA+AGG 0.497234 5.4:-47312391 None:intergenic
CTCACTCCGGTAACAATTGA+CGG 0.499475 5.4:+47312492 MS.gene010313:CDS
CCATCTTCGTCGGGAACCAC+AGG 0.500195 5.4:-47312543 None:intergenic
ATCCAAACCTAGTCAATCCT+TGG 0.508187 5.4:+47313805 MS.gene010313:CDS
CACATTGGAGATTGTTCCCA+GGG 0.513167 5.4:-47313102 None:intergenic
CAACCTGCCAAGGATTGACT+AGG 0.513736 5.4:-47313812 None:intergenic
TCGACAACGCGTGGCTGAGG+CGG 0.515631 5.4:-47312243 None:intergenic
AAAAGCTCCTGAGACGGCAT+TGG 0.515807 5.4:-47312672 None:intergenic
AAAATTTGGCAGACATGCGC+CGG 0.516610 5.4:+47312270 MS.gene010313:CDS
GAGGAGGAAGTCGAAGGTGC+AGG 0.519616 5.4:-47313849 None:intergenic
AAGATTGTTTCCATCTTCGT+CGG 0.521879 5.4:-47312553 None:intergenic
TATAGTTGAACCTCCAGACT+TGG 0.526294 5.4:-47312713 None:intergenic
TCGGGAACCACAGGAGGAGC+TGG 0.527028 5.4:-47312534 None:intergenic
ATAAAGAAGGAGGGTGAAGA+GGG 0.528210 5.4:+47312876 MS.gene010313:CDS
AGTCGAAGGTGCAGGTATGT+TGG 0.528919 5.4:-47313841 None:intergenic
TGGCTGAGGCGGCAGAGACA+TGG 0.530662 5.4:-47312232 None:intergenic
CCTCAGCCACGCGTTGTCGA+AGG 0.531551 5.4:+47312246 MS.gene010313:CDS
TGACTAGGTTTGGATTCTGC+GGG 0.534959 5.4:-47313797 None:intergenic
GATGAACTCTCAGCAAGTCT+AGG 0.537540 5.4:-47313900 None:intergenic
AGATTGTTTCCATCTTCGTC+GGG 0.537991 5.4:-47312552 None:intergenic
AGCTTCCAAAACCGCCGACT+CGG 0.538802 5.4:-47312922 None:intergenic
CCTGTGGTTCCCGACGAAGA+TGG 0.542076 5.4:+47312543 MS.gene010313:CDS
AAACCTAGTCAATCCTTGGC+AGG 0.546100 5.4:+47313809 MS.gene010313:CDS
CCAGACTTGGCCTTGAACAT+CGG 0.546481 5.4:-47312700 None:intergenic
AGGAGACTGTCTGAGGTAGT+GGG 0.547225 5.4:-47312371 None:intergenic
TTGAAGAATGATCAGTCCCT+GGG 0.547852 5.4:+47313086 MS.gene010313:CDS
TTTGGAAGCTGCGGAATTGG+CGG 0.551783 5.4:+47312935 MS.gene010313:CDS
ACACATTGGAGATTGTTCCC+AGG 0.561486 5.4:-47313103 None:intergenic
CATGAAAACAATGGAATCGT+CGG 0.561963 5.4:-47312811 None:intergenic
CTCAGCGGTTGATCTTCTTG+CGG 0.563754 5.4:+47312434 MS.gene010313:CDS
TTGGAAACTTCAACCTGCCA+AGG 0.564377 5.4:-47313822 None:intergenic
CAGTCCAGCCAGTAGAGAAA+CGG 0.568162 5.4:-47312761 None:intergenic
GACACCGTTTCTCTACTGGC+TGG 0.569539 5.4:+47312757 MS.gene010313:CDS
CAGCTTTGGAATCTTGACGC+CGG 0.579151 5.4:-47312295 None:intergenic
TTGACTAGGTTTGGATTCTG+CGG 0.579536 5.4:-47313798 None:intergenic
CGCCAAACTCATCCTCACTC+CGG 0.579944 5.4:+47312479 MS.gene010313:CDS
AATACGCCGGCACATAAAGA+AGG 0.582192 5.4:+47312863 MS.gene010313:CDS
GAGTTTGGCGAAAACTTCAT+CGG 0.584189 5.4:-47312466 None:intergenic
AAATCAGACCAATTATGACT+TGG 0.584394 5.4:-47312990 None:intergenic
TCTGCGGGAGAGTTATCTGT+TGG 0.584504 5.4:+47312840 MS.gene010313:CDS
AAATTCTACATTCAATTGCA+GGG 0.586656 5.4:+47313931 MS.gene010313:CDS
CATTCAATTGCAGGGTCGAG+TGG 0.586792 5.4:+47313939 MS.gene010313:CDS
ACTGTCTGAGGTAGTGGGGA+AGG 0.593489 5.4:-47312366 None:intergenic
TTGTACCTCGTGAATGCGCG+CGG 0.603239 5.4:+47312625 MS.gene010313:CDS
CGAAGAAGGATTAGACACAT+TGG 0.604406 5.4:-47313117 None:intergenic
CTGAAGAATGTTCCAATTAG+GGG 0.615518 5.4:-47313144 None:intergenic
CGTCAATTGTTACCGGAGTG+AGG 0.618461 5.4:-47312491 None:intergenic
GCGAAAACTTCATCGGTGTG+AGG 0.620277 5.4:-47312459 None:intergenic
TGTTGAGGTGAGATTGACAG+TGG 0.621134 5.4:-47313871 None:intergenic
ACGCCGGCACATAAAGAAGG+AGG 0.622809 5.4:+47312866 MS.gene010313:CDS
GAGGGAGAAGTTAACCGAGT+CGG 0.627289 5.4:+47312908 MS.gene010313:CDS
ATTGAATGTAGAATTTGTTG+GGG 0.627689 5.4:-47313924 None:intergenic
CCAAGAACCAGCTCCTCCTG+TGG 0.630584 5.4:+47312527 MS.gene010313:CDS
AAGAAGGAGGGTGAAGAGGG+AGG 0.636512 5.4:+47312879 MS.gene010313:CDS
ATGTTCAAGGCCAAGTCTGG+AGG 0.637068 5.4:+47312703 MS.gene010313:CDS
ATCAACCGCGCGCATTCACG+AGG 0.638655 5.4:-47312630 None:intergenic
CGCCGGCACATAAAGAAGGA+GGG 0.641777 5.4:+47312867 MS.gene010313:CDS
GGAGAAGTTAACCGAGTCGG+CGG 0.643380 5.4:+47312911 MS.gene010313:CDS
TGAGGTGAGATTGACAGTGG+AGG 0.644690 5.4:-47313868 None:intergenic
TCTTCGTCGGGAACCACAGG+AGG 0.646761 5.4:-47312540 None:intergenic
TCTATCAGGATTCTTGCGAA+AGG 0.647881 5.4:-47312736 None:intergenic
GGAGACTGTCTGAGGTAGTG+GGG 0.652562 5.4:-47312370 None:intergenic
TTGAATGTAGAATTTGTTGG+GGG 0.657320 5.4:-47313923 None:intergenic
AGTTATCTGTTGGAATACGC+CGG 0.657595 5.4:+47312850 MS.gene010313:CDS
TGAAGAAAGGAGACTGTCTG+AGG 0.660800 5.4:-47312378 None:intergenic
CTATCTCTGCATCATCTCAG+CGG 0.677491 5.4:+47312419 MS.gene010313:CDS
GCTTTGGAATCTTGACGCCG+GGG 0.693460 5.4:-47312293 None:intergenic
TGAATGTAGAATTTGTTGGG+GGG 0.703111 5.4:-47313922 None:intergenic
ACAGTGGAGGAGGAAGTCGA+AGG 0.704506 5.4:-47313855 None:intergenic
TTGTGTAAGAGTCTTGACGT+AGG 0.718748 5.4:-47312580 None:intergenic
ACTCCGGTAACAATTGACGG+TGG 0.780092 5.4:+47312495 MS.gene010313:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATCTAATTATGTATT+TGG - chr5.4:47313200-47313219 None:intergenic 10.0%
!! TATGATAAAAAGATAAAAAA+TGG - chr5.4:47313753-47313772 None:intergenic 10.0%
!! TTACAAAATATAATTACAAT+TGG - chr5.4:47313351-47313370 None:intergenic 10.0%
!!! ATTTTTTATCTTTTTATCAT+AGG + chr5.4:47313752-47313771 MS.gene010313:intron 10.0%
!! AATAACATTAAACGAAGAAA+TGG + chr5.4:47313456-47313475 MS.gene010313:intron 20.0%
!! CTATACATAACAGAAAATTA+TGG - chr5.4:47313374-47313393 None:intergenic 20.0%
!! TGTATATCTTTGTTCTTTAT+AGG + chr5.4:47313318-47313337 MS.gene010313:intron 20.0%
!! TTTCTTCGTTTAATGTTATT+CGG - chr5.4:47313456-47313475 None:intergenic 20.0%
!!! AATTGAATGTAGAATTTGTT+GGG - chr5.4:47313928-47313947 None:intergenic 20.0%
! AAATAAAGATCCTACTTAGA+AGG - chr5.4:47313645-47313664 None:intergenic 25.0%
! AAATTCTACATTCAATTGCA+GGG + chr5.4:47313931-47313950 MS.gene010313:CDS 25.0%
! CAATTGAATGTAGAATTTGT+TGG - chr5.4:47313929-47313948 None:intergenic 25.0%
! CTGAAACTATAATTAGATCA+TGG + chr5.4:47313272-47313291 MS.gene010313:intron 25.0%
! TGTATTATCCAAGTCATAAT+TGG + chr5.4:47312982-47313001 MS.gene010313:CDS 25.0%
!! AGAGTTTTTCATGAAAACAA+TGG - chr5.4:47312823-47312842 None:intergenic 25.0%
!! CTTTTTATCATAGGTTAACT+GGG + chr5.4:47313761-47313780 MS.gene010313:intron 25.0%
!! TCTTTTTATCATAGGTTAAC+TGG + chr5.4:47313760-47313779 MS.gene010313:intron 25.0%
!!! ATTGAATGTAGAATTTGTTG+GGG - chr5.4:47313927-47313946 None:intergenic 25.0%
AAATCAGACCAATTATGACT+TGG - chr5.4:47312993-47313012 None:intergenic 30.0%
ACGTATTGTAGTCATAGATA+AGG + chr5.4:47313512-47313531 MS.gene010313:intron 30.0%
AGAATGAAGATCATGAAGAA+AGG - chr5.4:47312394-47312413 None:intergenic 30.0%
AGTTTAAGAACCTTCTAAGT+AGG + chr5.4:47313632-47313651 MS.gene010313:intron 30.0%
ATTAAGTTCGAGACTTAATG+TGG - chr5.4:47313603-47313622 None:intergenic 30.0%
CAAATTCTACATTCAATTGC+AGG + chr5.4:47313930-47313949 MS.gene010313:CDS 30.0%
CGTATTGTAGTCATAGATAA+GGG + chr5.4:47313513-47313532 MS.gene010313:intron 30.0%
GTATTGTAGTCATAGATAAG+GGG + chr5.4:47313514-47313533 MS.gene010313:intron 30.0%
TATGTAATTGCAAAACCACT+CGG - chr5.4:47313403-47313422 None:intergenic 30.0%
! ATGAAGATTGTTTGGAATTC+TGG + chr5.4:47313044-47313063 MS.gene010313:CDS 30.0%
! GGCATTGGATTGTTAAAATT+TGG - chr5.4:47312660-47312679 None:intergenic 30.0%
! GTCGTTTACTATTTTCCAAA+AGG + chr5.4:47312324-47312343 MS.gene010313:CDS 30.0%
! TGAAGATTGTTTGGAATTCT+GGG + chr5.4:47313045-47313064 MS.gene010313:CDS 30.0%
! TGTTTTCATGAAAAACTCTG+CGG + chr5.4:47312824-47312843 MS.gene010313:CDS 30.0%
!! TCATAATTGGTCTGATTTTG+TGG + chr5.4:47312995-47313014 MS.gene010313:CDS 30.0%
!! TTGAATGTAGAATTTGTTGG+GGG - chr5.4:47313926-47313945 None:intergenic 30.0%
AAGATTGTTTCCATCTTCGT+CGG - chr5.4:47312556-47312575 None:intergenic 35.0%
ACCTGAAGAATGTTCCAATT+AGG - chr5.4:47313149-47313168 None:intergenic 35.0%
ATCTATGACTACAATACGTC+CGG - chr5.4:47313511-47313530 None:intergenic 35.0%
ATGACGCTATGAAGATTGTT+TGG + chr5.4:47313036-47313055 MS.gene010313:CDS 35.0%
ATTAAACGAAGAAATGGAGC+TGG + chr5.4:47313462-47313481 MS.gene010313:intron 35.0%
CATGAAAACAATGGAATCGT+CGG - chr5.4:47312814-47312833 None:intergenic 35.0%
CCTGAAGAATGTTCCAATTA+GGG - chr5.4:47313148-47313167 None:intergenic 35.0%
CTGAAGAATGTTCCAATTAG+GGG - chr5.4:47313147-47313166 None:intergenic 35.0%
TCATAACAAAGCACAATGTC+CGG - chr5.4:47313564-47313583 None:intergenic 35.0%
TTATCATAGGTTAACTGGGA+TGG + chr5.4:47313765-47313784 MS.gene010313:intron 35.0%
! AAGTCTAGGAGATTTTGTTG+AGG - chr5.4:47313889-47313908 None:intergenic 35.0%
! CTATTTTCCAAAAGGTCACT+TGG + chr5.4:47312332-47312351 MS.gene010313:CDS 35.0%
! GTTTTCATGAAAAACTCTGC+GGG + chr5.4:47312825-47312844 MS.gene010313:CDS 35.0%
!! TAATTGGTCTGATTTTGTGG+TGG + chr5.4:47312998-47313017 MS.gene010313:CDS 35.0%
!! TGAATGTAGAATTTGTTGGG+GGG - chr5.4:47313925-47313944 None:intergenic 35.0%
AGATTGTTTCCATCTTCGTC+GGG - chr5.4:47312555-47312574 None:intergenic 40.0%
AGTTATCTGTTGGAATACGC+CGG + chr5.4:47312850-47312869 MS.gene010313:CDS 40.0%
ATAAAGAAGGAGGGTGAAGA+GGG + chr5.4:47312876-47312895 MS.gene010313:CDS 40.0%
ATCCAAACCTAGTCAATCCT+TGG + chr5.4:47313805-47313824 MS.gene010313:CDS 40.0%
CAATTAGGGGAATTCGAAGA+AGG - chr5.4:47313134-47313153 None:intergenic 40.0%
CCCTAATTGGAACATTCTTC+AGG + chr5.4:47313145-47313164 MS.gene010313:CDS 40.0%
CGAAGAAGGATTAGACACAT+TGG - chr5.4:47313120-47313139 None:intergenic 40.0%
CTTCTTCGAATTCCCCTAAT+TGG + chr5.4:47313132-47313151 MS.gene010313:CDS 40.0%
GAGTTTGGCGAAAACTTCAT+CGG - chr5.4:47312469-47312488 None:intergenic 40.0%
GTTCGAGACTTAATGTGGTA+TGG - chr5.4:47313598-47313617 None:intergenic 40.0%
TAAACGACTGAGTTCAGCTT+TGG - chr5.4:47312312-47312331 None:intergenic 40.0%
TATAGTTGAACCTCCAGACT+TGG - chr5.4:47312716-47312735 None:intergenic 40.0%
TATGTATAGCTTTCTCCGAG+TGG + chr5.4:47313385-47313404 MS.gene010313:intron 40.0%
TCTATCAGGATTCTTGCGAA+AGG - chr5.4:47312739-47312758 None:intergenic 40.0%
TTGAAGAATGATCAGTCCCT+GGG + chr5.4:47313086-47313105 MS.gene010313:CDS 40.0%
TTGACTAGGTTTGGATTCTG+CGG - chr5.4:47313801-47313820 None:intergenic 40.0%
TTGTGTAAGAGTCTTGACGT+AGG - chr5.4:47312583-47312602 None:intergenic 40.0%
TTTGAAGAATGATCAGTCCC+TGG + chr5.4:47313085-47313104 MS.gene010313:CDS 40.0%
TTTGGATTCTGCGGGATATT+AGG - chr5.4:47313792-47313811 None:intergenic 40.0%
! CTTTTTCTCACCGATGTTCA+AGG + chr5.4:47312690-47312709 MS.gene010313:CDS 40.0%
AAAATTTGGCAGACATGCGC+CGG + chr5.4:47312270-47312289 MS.gene010313:CDS 45.0%
AAACCTAGTCAATCCTTGGC+AGG + chr5.4:47313809-47313828 MS.gene010313:CDS 45.0%
AATACGCCGGCACATAAAGA+AGG + chr5.4:47312863-47312882 MS.gene010313:CDS 45.0%
ACACATTGGAGATTGTTCCC+AGG - chr5.4:47313106-47313125 None:intergenic 45.0%
ACACCACCGTCAATTGTTAC+CGG - chr5.4:47312501-47312520 None:intergenic 45.0%
CACATTGGAGATTGTTCCCA+GGG - chr5.4:47313105-47313124 None:intergenic 45.0%
CATAAAGAAGGAGGGTGAAG+AGG + chr5.4:47312875-47312894 MS.gene010313:CDS 45.0%
CTATCTCTGCATCATCTCAG+CGG + chr5.4:47312419-47312438 MS.gene010313:CDS 45.0%
CTCACTCCGGTAACAATTGA+CGG + chr5.4:47312492-47312511 MS.gene010313:CDS 45.0%
GATAGACACCGTTTCTCTAC+TGG + chr5.4:47312753-47312772 MS.gene010313:CDS 45.0%
GATGAACTCTCAGCAAGTCT+AGG - chr5.4:47313903-47313922 None:intergenic 45.0%
GCGTTGTCGAAGGCAAAATT+TGG + chr5.4:47312256-47312275 MS.gene010313:CDS 45.0%
GTAGAGAAACGGTGTCTATC+AGG - chr5.4:47312753-47312772 None:intergenic 45.0%
GTGAGAAAAAGCTCCTGAGA+CGG - chr5.4:47312681-47312700 None:intergenic 45.0%
TAACAATCCAATGCCGTCTC+AGG + chr5.4:47312665-47312684 MS.gene010313:CDS 45.0%
TCACTTGAAGCCAGTTAGTC+CGG + chr5.4:47313542-47313561 MS.gene010313:intron 45.0%
TGAAGAAAGGAGACTGTCTG+AGG - chr5.4:47312381-47312400 None:intergenic 45.0%
TGACTAGGTTTGGATTCTGC+GGG - chr5.4:47313800-47313819 None:intergenic 45.0%
TGCCAAGGATTGACTAGGTT+TGG - chr5.4:47313810-47313829 None:intergenic 45.0%
TGTTGAGGTGAGATTGACAG+TGG - chr5.4:47313874-47313893 None:intergenic 45.0%
TTGACCGCAAACAGTTAGTC+CGG + chr5.4:47313489-47313508 MS.gene010313:intron 45.0%
TTGGAAACTTCAACCTGCCA+AGG - chr5.4:47313825-47313844 None:intergenic 45.0%
! GCATGTTCCAAGTGACCTTT+TGG - chr5.4:47312342-47312361 None:intergenic 45.0%
!!! TACTGGCTGGACTGGTTTTA+GGG + chr5.4:47312770-47312789 MS.gene010313:CDS 45.0%
AAAAGCTCCTGAGACGGCAT+TGG - chr5.4:47312675-47312694 None:intergenic 50.0%
ACGTCCGGACTAACTGTTTG+CGG - chr5.4:47313496-47313515 None:intergenic 50.0%
ACTCCGGTAACAATTGACGG+TGG + chr5.4:47312495-47312514 MS.gene010313:CDS 50.0%
AGCACAATGTCCGGACTAAC+TGG - chr5.4:47313555-47313574 None:intergenic 50.0%
ATGTTCAAGGCCAAGTCTGG+AGG + chr5.4:47312703-47312722 MS.gene010313:CDS 50.0%
CAACCTGCCAAGGATTGACT+AGG - chr5.4:47313815-47313834 None:intergenic 50.0%
CACCCTCCTTCTTTATGTGC+CGG - chr5.4:47312872-47312891 None:intergenic 50.0%
CAGTCCAGCCAGTAGAGAAA+CGG - chr5.4:47312764-47312783 None:intergenic 50.0%
CATTCAATTGCAGGGTCGAG+TGG + chr5.4:47313939-47313958 MS.gene010313:CDS 50.0%
CGCTGAGATGATGCAGAGAT+AGG - chr5.4:47312421-47312440 None:intergenic 50.0%
CGTCAATTGTTACCGGAGTG+AGG - chr5.4:47312494-47312513 None:intergenic 50.0%
CTCAGCGGTTGATCTTCTTG+CGG + chr5.4:47312434-47312453 MS.gene010313:CDS 50.0%
GAGGGAGAAGTTAACCGAGT+CGG + chr5.4:47312908-47312927 MS.gene010313:CDS 50.0%
GCGAAAACTTCATCGGTGTG+AGG - chr5.4:47312462-47312481 None:intergenic 50.0%
TACCGGAGTGAGGATGAGTT+TGG - chr5.4:47312484-47312503 None:intergenic 50.0%
TCTGCGGGAGAGTTATCTGT+TGG + chr5.4:47312840-47312859 MS.gene010313:CDS 50.0%
TGAGGTGAGATTGACAGTGG+AGG - chr5.4:47313871-47313890 None:intergenic 50.0%
TTTGGAAGCTGCGGAATTGG+CGG + chr5.4:47312935-47312954 MS.gene010313:CDS 50.0%
! AAGGAGACTGTCTGAGGTAG+TGG - chr5.4:47312375-47312394 None:intergenic 50.0%
! AGGAGACTGTCTGAGGTAGT+GGG - chr5.4:47312374-47312393 None:intergenic 50.0%
! AGTCGAAGGTGCAGGTATGT+TGG - chr5.4:47313844-47313863 None:intergenic 50.0%
! ATTTTGCCTTCGACAACGCG+TGG - chr5.4:47312255-47312274 None:intergenic 50.0%
!! AGCTTTGGAATCTTGACGCC+GGG - chr5.4:47312297-47312316 None:intergenic 50.0%
!! CAGCTTTGGAATCTTGACGC+CGG - chr5.4:47312298-47312317 None:intergenic 50.0%
!! CCAGACTTGGCCTTGAACAT+CGG - chr5.4:47312703-47312722 None:intergenic 50.0%
!!! CTACTGGCTGGACTGGTTTT+AGG + chr5.4:47312769-47312788 MS.gene010313:CDS 50.0%
!!! GGTTTTGGAAGCTGCGGAAT+TGG + chr5.4:47312932-47312951 MS.gene010313:CDS 50.0%
AAGAAGGAGGGTGAAGAGGG+AGG + chr5.4:47312879-47312898 MS.gene010313:CDS 55.0%
ACAGTGGAGGAGGAAGTCGA+AGG - chr5.4:47313858-47313877 None:intergenic 55.0%
ACGCCGGCACATAAAGAAGG+AGG + chr5.4:47312866-47312885 MS.gene010313:CDS 55.0%
CCGATGTTCAAGGCCAAGTC+TGG + chr5.4:47312700-47312719 MS.gene010313:CDS 55.0%
CGCCAAACTCATCCTCACTC+CGG + chr5.4:47312479-47312498 MS.gene010313:CDS 55.0%
CGCCGGCACATAAAGAAGGA+GGG + chr5.4:47312867-47312886 MS.gene010313:CDS 55.0%
GACACCGTTTCTCTACTGGC+TGG + chr5.4:47312757-47312776 MS.gene010313:CDS 55.0%
GGAGAAGTTAACCGAGTCGG+CGG + chr5.4:47312911-47312930 MS.gene010313:CDS 55.0%
GGTGAGATTGACAGTGGAGG+AGG - chr5.4:47313868-47313887 None:intergenic 55.0%
TGAAGAGGGAGGCTGGAAGA+GGG + chr5.4:47312890-47312909 MS.gene010313:CDS 55.0%
TTGTACCTCGTGAATGCGCG+CGG + chr5.4:47312625-47312644 MS.gene010313:CDS 55.0%
! AGCTTCCAAAACCGCCGACT+CGG - chr5.4:47312925-47312944 None:intergenic 55.0%
! CGTTTCTCTACTGGCTGGAC+TGG + chr5.4:47312762-47312781 MS.gene010313:CDS 55.0%
! GAGGAGCTGGTTCTTGGACT+TGG - chr5.4:47312524-47312543 None:intergenic 55.0%
! GGAGACTGTCTGAGGTAGTG+GGG - chr5.4:47312373-47312392 None:intergenic 55.0%
! GTTAACCGAGTCGGCGGTTT+TGG + chr5.4:47312917-47312936 MS.gene010313:CDS 55.0%
!! ACTGTCTGAGGTAGTGGGGA+AGG - chr5.4:47312369-47312388 None:intergenic 55.0%
!! GCTTTGGAATCTTGACGCCG+GGG - chr5.4:47312296-47312315 None:intergenic 55.0%
ATCAACCGCGCGCATTCACG+AGG - chr5.4:47312633-47312652 None:intergenic 60.0%
CCAAGAACCAGCTCCTCCTG+TGG + chr5.4:47312527-47312546 MS.gene010313:CDS 60.0%
CCATCTTCGTCGGGAACCAC+AGG - chr5.4:47312546-47312565 None:intergenic 60.0%
CCTGTGGTTCCCGACGAAGA+TGG + chr5.4:47312543-47312562 MS.gene010313:CDS 60.0%
GTGAAGAGGGAGGCTGGAAG+AGG + chr5.4:47312889-47312908 MS.gene010313:CDS 60.0%
TCTTCGTCGGGAACCACAGG+AGG - chr5.4:47312543-47312562 None:intergenic 60.0%
! CCACAGGAGGAGCTGGTTCT+TGG - chr5.4:47312530-47312549 None:intergenic 60.0%
! GAGGAGGAAGTCGAAGGTGC+AGG - chr5.4:47313852-47313871 None:intergenic 60.0%
!!! GTCGGCGGTTTTGGAAGCTG+CGG + chr5.4:47312926-47312945 MS.gene010313:CDS 60.0%
AGGAGGGTGAAGAGGGAGGC+TGG + chr5.4:47312883-47312902 MS.gene010313:CDS 65.0%
CCTCAGCCACGCGTTGTCGA+AGG + chr5.4:47312246-47312265 MS.gene010313:CDS 65.0%
CCTTCGACAACGCGTGGCTG+AGG - chr5.4:47312249-47312268 None:intergenic 65.0%
TCGACAACGCGTGGCTGAGG+CGG - chr5.4:47312246-47312265 None:intergenic 65.0%
TCGGGAACCACAGGAGGAGC+TGG - chr5.4:47312537-47312556 None:intergenic 65.0%
TGGAATCTTGACGCCGGGGC+CGG - chr5.4:47312292-47312311 None:intergenic 65.0%
! TGGCAGACATGCGCCGGCCC+CGG + chr5.4:47312276-47312295 MS.gene010313:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 47312234 47313980 47312234 ID=MS.gene010313
chr5.4 mRNA 47312234 47313980 47312234 ID=MS.gene010313.t1;Parent=MS.gene010313
chr5.4 exon 47312234 47313166 47312234 ID=MS.gene010313.t1.exon1;Parent=MS.gene010313.t1
chr5.4 CDS 47312234 47313166 47312234 ID=cds.MS.gene010313.t1;Parent=MS.gene010313.t1
chr5.4 exon 47313774 47313980 47313774 ID=MS.gene010313.t1.exon2;Parent=MS.gene010313.t1
chr5.4 CDS 47313774 47313980 47313774 ID=cds.MS.gene010313.t1;Parent=MS.gene010313.t1
Gene Sequence

>MS.gene010313

ATGTCTCTGCCGCCTCAGCCACGCGTTGTCGAAGGCAAAATTTGGCAGACATGCGCCGGCCCCGGCGTCAAGATTCCAAAGCTGAACTCAGTCGTTTACTATTTTCCAAAAGGTCACTTGGAACATGCATGTCCTTCCCCACTACCTCAGACAGTCTCCTTTCTTCATGATCTTCATTCTTCATCCTATCTCTGCATCATCTCAGCGGTTGATCTTCTTGCGGATCCTCACACCGATGAAGTTTTCGCCAAACTCATCCTCACTCCGGTAACAATTGACGGTGGTGTCCAAGTCCAAGAACCAGCTCCTCCTGTGGTTCCCGACGAAGATGGAAACAATCTTGTTTCCTACGTCAAGACTCTTACACAATCTGATTCTAATAATATATTCTTTGTACCTCGTGAATGCGCGCGGTTGATCTTTCCAAATTTTAACAATCCAATGCCGTCTCAGGAGCTTTTTCTCACCGATGTTCAAGGCCAAGTCTGGAGGTTCAACTATACCTTTCGCAAGAATCCTGATAGACACCGTTTCTCTACTGGCTGGACTGGTTTTAGGGACGACAAGAAACTCGTCGCCGACGATTCCATTGTTTTCATGAAAAACTCTGCGGGAGAGTTATCTGTTGGAATACGCCGGCACATAAAGAAGGAGGGTGAAGAGGGAGGCTGGAAGAGGGAGAAGTTAACCGAGTCGGCGGTTTTGGAAGCTGCGGAATTGGCGGAAGCGAACAAAGCGTTTGAAGTTGTGTATTATCCAAGTCATAATTGGTCTGATTTTGTGGTGGATGCTAAGACTGTTGATGACGCTATGAAGATTGTTTGGAATTCTGGGATGAGAGTGAAGCTTCTTTTGAAGAATGATCAGTCCCTGGGAACAATCTCCAATGTGTCTAATCCTTCTTCGAATTCCCCTAATTGGAACATTCTTCAGGTACATATTTCATAATGTTTACAGTTTTTACCAAATACATAATTAGATTATTTGTTTGATAAATATTAGTGTTATAGTTGATACATGATTAATTAGATGTTGAAACTGAAACTATAATTAGATCATGGATCGTATACTAAAATTGCTGCTATGTATATCTTTGTTCTTTATAGGATAATTTCCAATTGTAATTATATTTTGTAACCATAATTTTCTGTTATGTATAGCTTTCTCCGAGTGGTTTTGCAATTACATATAAAATAAAGAATTCACAATTGTCATCAAACCGAATAACATTAAACGAAGAAATGGAGCTGGCTTTTTGACCGCAAACAGTTAGTCCGGACGTATTGTAGTCATAGATAAGGGGATGATTCACTTGAAGCCAGTTAGTCCGGACATTGTGCTTTGTTATGAGTGTTGTGTTACCATACCACATTAAGTCTCGAACTTAATTGATATGCAAGTTTAAGAACCTTCTAAGTAGGATCTTTATTTTAGCTCATGATATTAATTGATGTCAAAATACACATATACTATATATAATTTATCATATATTTCAAATGATCGATATTGACTCTCACCATTTTTTATCTTTTTATCATAGGTTAACTGGGATGGACCTAATATCCCGCAGAATCCAAACCTAGTCAATCCTTGGCAGGTTGAAGTTTCCAACATACCTGCACCTTCGACTTCCTCCTCCACTGTCAATCTCACCTCAACAAAATCTCCTAGACTTGCTGAGAGTTCATCCCCCCAACAAATTCTACATTCAATTGCAGGGTCGAGTGGTACTTTGCCTGACGCGTAG
Protein sequence

>MS.gene010313.t1

MSLPPQPRVVEGKIWQTCAGPGVKIPKLNSVVYYFPKGHLEHACPSPLPQTVSFLHDLHSSSYLCIISAVDLLADPHTDEVFAKLILTPVTIDGGVQVQEPAPPVVPDEDGNNLVSYVKTLTQSDSNNIFFVPRECARLIFPNFNNPMPSQELFLTDVQGQVWRFNYTFRKNPDRHRFSTGWTGFRDDKKLVADDSIVFMKNSAGELSVGIRRHIKKEGEEGGWKREKLTESAVLEAAELAEANKAFEVVYYPSHNWSDFVVDAKTVDDAMKIVWNSGMRVKLLLKNDQSLGTISNVSNPSSNSPNWNILQVNWDGPNIPQNPNLVNPWQVEVSNIPAPSTSSSTVNLTSTKSPRLAESSSPQQILHSIAGSSGTLPDA