AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010315

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010315.t1 MTR_5g060630 91.365 498 40 1 13 507 26 523 0.0 879
MS.gene010315.t1 MTR_5g460740 91.365 498 40 1 13 507 26 523 0.0 879
MS.gene010315.t1 MTR_5g061220 46.591 528 182 11 6 502 22 480 4.20e-134 399
MS.gene010315.t1 MTR_5g060780 52.439 410 142 8 13 420 26 384 1.10e-117 352
MS.gene010315.t1 MTR_5g460920 52.439 410 142 8 13 420 26 384 5.49e-117 350
MS.gene010315.t1 MTR_5g061890 52.184 412 142 9 13 420 26 386 1.18e-116 349
MS.gene010315.t1 MTR_1g058210 50.513 390 139 8 13 398 26 365 1.28e-110 336
MS.gene010315.t1 MTR_5g040880 40.695 489 206 15 8 479 15 436 6.52e-95 298
MS.gene010315.t1 MTR_2g024430 41.807 476 192 17 13 479 34 433 3.91e-89 282
MS.gene010315.t1 MTR_5g082140 38.936 470 201 15 13 473 26 418 3.39e-85 270
MS.gene010315.t1 MTR_2g006270 38.171 503 193 19 13 500 27 426 1.02e-81 261
MS.gene010315.t1 MTR_5g060770 43.133 415 124 9 50 460 9 315 1.14e-77 248
MS.gene010315.t1 MTR_2g006380 36.653 502 195 19 13 500 34 426 2.28e-75 245
MS.gene010315.t1 MTR_5g040740 44.868 341 132 13 8 334 16 314 1.70e-65 218
MS.gene010315.t1 MTR_3g073420 32.482 548 244 17 14 505 34 511 6.46e-65 221
MS.gene010315.t1 MTR_5g062960 48.918 231 64 3 248 478 2 178 2.34e-61 200
MS.gene010315.t1 MTR_5g460910 37.915 422 128 10 43 460 19 310 1.27e-58 197
MS.gene010315.t1 MTR_5g062970 54.589 207 61 7 13 215 52 229 6.73e-55 184
MS.gene010315.t1 MTR_2g094570 32.791 430 198 13 14 391 26 416 2.04e-53 191
MS.gene010315.t1 MTR_2g094570 32.791 430 198 13 14 391 26 416 2.15e-53 192
MS.gene010315.t1 MTR_2g094570 32.791 430 198 13 14 391 82 472 3.82e-53 192
MS.gene010315.t1 MTR_4g058930 32.464 422 191 14 13 379 26 408 9.59e-48 176
MS.gene010315.t1 MTR_4g058930 32.464 422 191 14 13 379 26 408 1.54e-47 175
MS.gene010315.t1 MTR_1g064430 29.532 491 232 15 14 455 42 467 4.93e-47 174
MS.gene010315.t1 MTR_1g064430 29.532 491 232 15 14 455 55 480 5.42e-47 174
MS.gene010315.t1 MTR_1g094960 30.502 518 242 20 14 478 34 486 1.68e-45 169
MS.gene010315.t1 MTR_7g101280 29.109 505 229 21 13 459 45 478 6.11e-42 159
MS.gene010315.t1 MTR_0041s0110 29.109 505 229 21 13 459 45 478 6.11e-42 159
MS.gene010315.t1 MTR_4g021580 28.462 390 232 11 14 395 34 384 1.09e-39 152
MS.gene010315.t1 MTR_4g021580 28.462 390 232 11 14 395 34 384 1.57e-39 152
MS.gene010315.t1 MTR_2g014770 29.379 354 201 10 15 354 69 387 2.80e-38 148
MS.gene010315.t1 MTR_2g014770 29.379 354 201 10 15 354 69 387 3.97e-38 148
MS.gene010315.t1 MTR_2g093740 30.471 361 196 13 15 354 72 398 7.41e-37 145
MS.gene010315.t1 MTR_2g043250 27.155 464 287 12 14 466 38 461 1.18e-36 145
MS.gene010315.t1 MTR_2g093740 30.471 361 196 13 15 354 72 398 1.50e-36 144
MS.gene010315.t1 MTR_2g093740 30.471 361 196 13 15 354 72 398 1.51e-36 144
MS.gene010315.t1 MTR_7g062540 27.062 388 229 13 15 387 30 378 1.58e-36 144
MS.gene010315.t1 MTR_7g101275 31.454 337 185 12 13 339 6 306 1.90e-35 137
MS.gene010315.t1 MTR_4g124900 28.729 362 201 9 14 359 38 358 7.79e-35 139
MS.gene010315.t1 MTR_4g124900 28.650 363 202 9 14 360 38 359 1.00e-34 139
MS.gene010315.t1 MTR_2g005240 29.745 353 198 11 15 354 39 354 1.13e-34 138
MS.gene010315.t1 MTR_4g088210 28.161 348 217 9 15 354 54 376 1.17e-33 135
MS.gene010315.t1 MTR_4g088210 28.161 348 217 9 15 354 54 376 1.19e-33 135
MS.gene010315.t1 MTR_2g093740 29.412 374 196 14 15 354 72 411 1.21e-33 135
MS.gene010315.t1 MTR_2g093740 29.412 374 196 14 15 354 72 411 1.80e-33 135
MS.gene010315.t1 MTR_2g093740 29.412 374 196 14 15 354 72 411 1.83e-33 135
MS.gene010315.t1 MTR_8g100050 28.841 371 208 12 15 369 75 405 2.36e-33 134
MS.gene010315.t1 MTR_8g100050 28.841 371 208 12 15 369 75 405 5.02e-33 134
MS.gene010315.t1 MTR_1g024025 30.971 381 202 15 13 370 57 399 7.00e-33 134
MS.gene010315.t1 MTR_8g079492 28.342 374 220 12 15 374 39 378 1.10e-31 130
MS.gene010315.t1 MTR_8g079492 28.342 374 220 12 15 374 39 378 1.18e-31 130
MS.gene010315.t1 MTR_8g027440 28.933 356 205 13 15 360 45 362 3.82e-31 127
MS.gene010315.t1 MTR_4g060460 28.857 350 216 10 15 354 70 396 4.26e-31 128
MS.gene010315.t1 MTR_4g060460 28.857 350 216 10 15 354 70 396 4.74e-31 128
MS.gene010315.t1 MTR_3g064050 27.895 380 227 13 15 380 42 388 1.07e-30 127
MS.gene010315.t1 MTR_5g076270 28.075 374 222 13 15 374 40 380 1.24e-30 126
MS.gene010315.t1 MTR_2g018690 27.321 377 223 12 14 374 38 379 1.79e-29 123
MS.gene010315.t1 MTR_0041s0120 29.060 351 187 13 13 339 6 318 1.30e-28 117
MS.gene010315.t1 MTR_8g101360 28.080 349 212 9 15 354 47 365 3.61e-28 119
MS.gene010315.t1 MTR_2g093740 31.835 267 151 10 107 354 14 268 1.71e-27 116
MS.gene010315.t1 MTR_2g093740 31.835 267 151 10 107 354 14 268 2.30e-27 116
MS.gene010315.t1 MTR_2g093740 30.357 280 151 11 107 354 14 281 2.30e-24 107
MS.gene010315.t1 MTR_2g093740 30.357 280 151 11 107 354 14 281 2.62e-24 107
MS.gene010315.t1 MTR_8g079492 30.534 262 164 8 128 377 44 299 2.85e-24 107
MS.gene010315.t1 MTR_8g446900 26.087 322 184 14 45 354 9 288 2.03e-22 99.4
MS.gene010315.t1 MTR_5g046120 34.058 138 61 4 37 172 12 121 3.35e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010315.t1 AT1G77850 35.323 402 185 11 6 366 22 389 4.10e-62 214
MS.gene010315.t1 AT1G77850 35.323 402 185 11 6 366 22 389 4.58e-62 213
MS.gene010315.t1 AT4G30080 32.678 407 199 12 14 376 34 409 3.84e-49 179
MS.gene010315.t1 AT2G28350 32.783 424 210 15 14 391 25 419 3.98e-48 177
MS.gene010315.t1 AT2G28350 32.783 424 210 15 14 391 25 419 4.85e-48 177
MS.gene010315.t1 AT2G46530 29.752 363 209 9 15 367 37 363 2.40e-42 159
MS.gene010315.t1 AT2G46530 29.752 363 209 9 15 367 58 384 3.74e-42 159
MS.gene010315.t1 AT2G33860 32.011 353 193 12 15 354 70 388 1.46e-39 152
MS.gene010315.t1 AT4G23980 29.683 347 201 9 17 354 30 342 3.22e-39 151
MS.gene010315.t1 AT4G23980 29.683 347 201 9 17 354 30 342 3.32e-39 151
MS.gene010315.t1 AT3G61830 29.517 393 224 13 17 393 43 398 2.23e-38 148
MS.gene010315.t1 AT1G59750 30.120 415 225 15 15 409 38 407 6.33e-37 145
MS.gene010315.t1 AT1G59750 29.594 419 227 15 15 409 38 412 1.24e-35 141
MS.gene010315.t1 AT1G59750 29.594 419 227 15 15 409 38 412 1.24e-35 141
MS.gene010315.t1 AT1G59750 29.976 417 225 16 15 409 38 409 1.43e-35 140
MS.gene010315.t1 AT5G62000 29.539 369 204 13 17 369 79 407 9.82e-35 139
MS.gene010315.t1 AT5G62000 29.539 369 204 13 17 369 79 407 1.12e-34 139
MS.gene010315.t1 AT5G62000 29.539 369 204 13 17 369 79 407 1.12e-34 139
MS.gene010315.t1 AT5G62000 29.539 369 204 13 17 369 79 407 1.12e-34 139
MS.gene010315.t1 AT5G62000 29.539 369 204 13 17 369 79 407 1.12e-34 139
MS.gene010315.t1 AT5G20730 29.086 361 199 9 15 359 40 359 3.54e-34 137
MS.gene010315.t1 AT3G61830 29.558 362 205 11 45 393 23 347 4.12e-34 135
MS.gene010315.t1 AT1G34390 28.329 353 207 9 9 354 37 350 2.04e-33 134
MS.gene010315.t1 AT5G60450 28.652 356 207 12 15 354 57 381 1.03e-32 133
MS.gene010315.t1 AT5G60450 29.722 360 198 13 15 354 81 405 1.08e-32 133
MS.gene010315.t1 AT5G20730 29.006 362 199 10 15 359 40 360 1.19e-32 133
MS.gene010315.t1 AT5G20730 29.006 362 199 10 15 359 40 360 1.65e-32 132
MS.gene010315.t1 AT1G19220 26.611 357 207 8 14 354 38 355 2.36e-32 132
MS.gene010315.t1 AT1G34310 28.451 355 206 10 9 354 37 352 2.56e-32 130
MS.gene010315.t1 AT5G37020 27.824 363 211 12 15 359 38 367 2.67e-32 131
MS.gene010315.t1 AT5G37020 27.824 363 211 12 15 359 38 367 3.81e-32 131
MS.gene010315.t1 AT1G30330 28.198 383 218 14 14 377 40 384 2.21e-31 129
MS.gene010315.t1 AT1G30330 28.198 383 218 14 14 377 40 384 2.21e-31 129
MS.gene010315.t1 AT1G30330 28.198 383 218 14 14 377 38 382 2.35e-31 129
MS.gene010315.t1 AT1G19850 29.863 365 207 14 13 360 68 400 7.87e-31 127
MS.gene010315.t1 AT1G19850 29.863 365 207 14 13 360 57 389 9.02e-31 127
MS.gene010315.t1 AT1G34410 27.324 355 210 10 9 354 37 352 1.00e-30 126
MS.gene010315.t1 AT1G35240 27.222 360 204 11 9 354 37 352 1.24e-30 125
MS.gene010315.t1 AT1G35520 28.451 355 206 11 9 354 37 352 1.49e-30 125
MS.gene010315.t1 AT2G33860 32.627 236 144 7 128 354 2 231 2.82e-30 123
MS.gene010315.t1 AT2G46530 31.496 254 154 7 124 367 33 276 5.60e-30 123
MS.gene010315.t1 AT1G35540 26.944 360 205 10 9 354 37 352 1.26e-29 123
MS.gene010315.t1 AT1G34170 28.814 354 196 13 15 354 41 352 2.99e-27 115
MS.gene010315.t1 AT5G37020 29.555 247 154 7 128 359 31 272 1.22e-24 108
MS.gene010315.t1 AT1G34170 27.401 354 175 12 15 354 41 326 7.12e-22 99.0
MS.gene010315.t1 AT1G34170 27.401 354 175 12 15 354 41 326 1.21e-21 98.6
MS.gene010315.t1 AT1G43950 30.769 221 112 10 9 223 37 222 1.89e-18 84.7

Find 145 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTCCATCGTTCTCTTCT+TGG 0.162944 5.4:-47253705 None:intergenic
TGCTGTCAAGATTCCGAAAT+TGG 0.211577 5.4:+47253448 MS.gene010315:CDS
TCTCTTTATCATAGGTTAAC+TGG 0.215844 5.4:+47255491 MS.gene010315:intron
GATAAGGGTGATTGTTTCAT+TGG 0.257124 5.4:+47255904 MS.gene010315:CDS
AAGGATCCCATTGTTGAATA+AGG 0.268591 5.4:-47255784 None:intergenic
GCATGTTCCAAGTGACCTTC+TGG 0.268996 5.4:-47253490 None:intergenic
CATCTCATCTGTTGATCTTC+TGG 0.271893 5.4:+47253579 MS.gene010315:CDS
ATGAAGAATGGTTGGGAATC+TGG 0.280865 5.4:+47254273 MS.gene010315:CDS
CCTCAGGAGGAGCTGGTTCT+TGG 0.290438 5.4:-47253744 None:intergenic
GGTAATAGAGGCGGTGGAAT+TGG 0.297777 5.4:-47255625 None:intergenic
GAGGAGCTGGTTCTTGGATT+TGG 0.300688 5.4:-47253669 None:intergenic
AGTCAGGAGGAGCTGGTTCT+TGG 0.317855 5.4:-47253675 None:intergenic
GATAATAGTTTGAACTCCTT+TGG 0.327620 5.4:+47255982 MS.gene010315:CDS
TGCCAAGGATTGACGCGGTT+TGG 0.331418 5.4:-47255538 None:intergenic
TGTATTATCCGACGGCTAAT+TGG 0.337447 5.4:+47254211 MS.gene010315:CDS
TCACTTGGTGCGTGGTTCAT+TGG 0.346804 5.4:-47256007 None:intergenic
TTTGGATTCTGCGAGATATC+AGG 0.347838 5.4:-47255520 None:intergenic
TATCAGAATTCAACAATGTT+TGG 0.347991 5.4:-47255810 None:intergenic
CCGCCTTCCTCTTCAGCTGC+AGG 0.349653 5.4:-47254111 None:intergenic
TCCTAGCTGGCGTATGCTTC+AGG 0.352282 5.4:+47254404 MS.gene010315:CDS
GGAATCGGTGGATAAGAATA+TGG 0.352908 5.4:-47255703 None:intergenic
ATAATAGTTTGAACTCCTTT+GGG 0.354875 5.4:+47255983 MS.gene010315:CDS
AAGAAACTCAGGTTTACTTC+TGG 0.365878 5.4:+47253979 MS.gene010315:CDS
CCGACATTCAAGACGTAGTC+TGG 0.367274 5.4:+47253932 MS.gene010315:CDS
AAGAATCTGATGCAGATAAA+TGG 0.370769 5.4:-47255867 None:intergenic
TTTCATTGGAAACAGATCTA+TGG 0.376833 5.4:+47255918 MS.gene010315:CDS
GATAAGAATATGGCATTTGT+AGG 0.383476 5.4:-47255600 None:intergenic
GGAATTGGAGGATAAGAATA+TGG 0.385197 5.4:-47255610 None:intergenic
CCATCGTTCTCTTCTTGGTC+GGG 0.392490 5.4:-47253700 None:intergenic
CTTTCCCAATCTTGACTTGG+AGG 0.393297 5.4:+47253879 MS.gene010315:CDS
AGGATCCCATTGTTGAATAA+GGG 0.394605 5.4:-47255783 None:intergenic
GATAAGAATATGGAATTTGT+AGG 0.397197 5.4:-47255693 None:intergenic
AATTCCGACAAATATCTTTC+CGG 0.399060 5.4:-47254070 None:intergenic
AGGAGCTGTGTTTCAATGTC+AGG 0.401996 5.4:-47253523 None:intergenic
TCCATCGTTCTCTTCTTGGT+CGG 0.402482 5.4:-47253701 None:intergenic
CTCTTTATCATAGGTTAACT+GGG 0.403608 5.4:+47255492 MS.gene010315:intron
CTGGCTCCCAGCATGCCGTC+TGG 0.405966 5.4:-47255838 None:intergenic
ATTCAACAATGTTTGGTTCA+AGG 0.406117 5.4:-47255803 None:intergenic
CGGAATCTTGACAGCAGGTC+CGG 0.415975 5.4:-47253441 None:intergenic
GCTATCATCGTCTTCTTCAT+CGG 0.416752 5.4:-47253770 None:intergenic
TGCAATAGAGGCGGTGGAAT+CGG 0.418572 5.4:-47255718 None:intergenic
GAGGAGCTGGTTCTTGGACT+TGG 0.420907 5.4:-47253738 None:intergenic
CTATCATCGTCTTCTTCATC+GGG 0.426116 5.4:-47253769 None:intergenic
CGCCAGCTAGGAACATTAGA+AGG 0.426148 5.4:-47254393 None:intergenic
TCTTCTTGGTCGGGAAAGTC+AGG 0.427116 5.4:-47253691 None:intergenic
TGGATCCTCCAAGTCAAGAT+TGG 0.427196 5.4:-47253884 None:intergenic
ATAAGAATATGGCATTTGTA+GGG 0.430507 5.4:-47255599 None:intergenic
GACATAGAAGACTGCAATAG+AGG 0.437550 5.4:-47255730 None:intergenic
AGTTAAAGATGCTGTGGAAT+TGG 0.445090 5.4:+47254161 MS.gene010315:CDS
GGGAATGGTAATAGAGGCGG+TGG 0.449146 5.4:-47255631 None:intergenic
ATCCAAACCGCGTCAATCCT+TGG 0.458623 5.4:+47255536 MS.gene010315:CDS
GAATAAGGGAACTCTGTCAT+AGG 0.460002 5.4:-47255769 None:intergenic
CGCCTTCCTCTTCAGCTGCA+GGG 0.460616 5.4:-47254110 None:intergenic
TCTTCTTCATCGGGAACCTC+AGG 0.467124 5.4:-47253760 None:intergenic
TACCAGAGTGAGGAGCAGTT+TGG 0.469806 5.4:-47253629 None:intergenic
GAGTTCCCTTATTCAACAAT+GGG 0.473672 5.4:+47255778 MS.gene010315:CDS
CATGTTCCAAGTGACCTTCT+GGG 0.475680 5.4:-47253489 None:intergenic
CGGGAAAAGTGAGAATTTAA+CGG 0.481743 5.4:+47254131 MS.gene010315:CDS
ATGGAGGGCGAAGACATAGA+AGG 0.491229 5.4:-47253543 None:intergenic
GTCCACTGTGAGTATCAGAT+TGG 0.492170 5.4:-47253825 None:intergenic
ATAAGAATATGGAATTTGTA+GGG 0.493993 5.4:-47255692 None:intergenic
GGATCCTCCAAGTCAAGATT+GGG 0.497543 5.4:-47253883 None:intergenic
CTACTTCCCAGAAGGTCACT+TGG 0.497684 5.4:+47253483 MS.gene010315:CDS
ACGAGACTATGAAGAATGGT+TGG 0.500722 5.4:+47254265 MS.gene010315:CDS
GAATGGTTGGGAATCTGGAA+TGG 0.503016 5.4:+47254278 MS.gene010315:CDS
GAAGACATATTATCAGCCTA+AGG 0.506655 5.4:+47254344 MS.gene010315:CDS
CGAGACTATGAAGAATGGTT+GGG 0.506723 5.4:+47254266 MS.gene010315:CDS
AAGAATATGGCATTTGTAGG+GGG 0.508074 5.4:-47255597 None:intergenic
AACTCAGGTTTACTTCTGGT+TGG 0.509116 5.4:+47253983 MS.gene010315:CDS
GATCTTTCCCAATCTTGACT+TGG 0.511867 5.4:+47253876 MS.gene010315:CDS
AATGGTTGGGAATCTGGAAT+GGG 0.515696 5.4:+47254279 MS.gene010315:CDS
TCGGGAACCTCAGGAGGAGC+TGG 0.515759 5.4:-47253751 None:intergenic
TTCCTTCTAATGTTCCTAGC+TGG 0.515821 5.4:+47254391 MS.gene010315:CDS
AGATATTTGTCGGAATTCGC+AGG 0.516804 5.4:+47254076 MS.gene010315:CDS
AAAGATGCTGTGGAATTGGC+TGG 0.518790 5.4:+47254165 MS.gene010315:CDS
TGCCAAACTGCTCCTCACTC+TGG 0.523844 5.4:+47253627 MS.gene010315:CDS
ATGTTAGAGATTGTCCCCTT+AGG 0.526512 5.4:-47254360 None:intergenic
TTGGATTTGGACGCCACCAT+CGG 0.530656 5.4:-47253656 None:intergenic
TCGGGAAAGTCAGGAGGAGC+TGG 0.536223 5.4:-47253682 None:intergenic
GAGAGCTTCTGAGACTGCAT+TGG 0.537366 5.4:-47253904 None:intergenic
TATGTACCCTGCAGCTGAAG+AGG 0.538322 5.4:+47254104 MS.gene010315:CDS
AAACCGCGTCAATCCTTGGC+AGG 0.540194 5.4:+47255540 MS.gene010315:CDS
AATAGAGGCGGTGGAATTGG+AGG 0.543731 5.4:-47255622 None:intergenic
AATTTCGGAATCTTGACAGC+AGG 0.543760 5.4:-47253446 None:intergenic
GAAGACTGCAATAGAGGCGG+TGG 0.544397 5.4:-47255724 None:intergenic
AGAGTTCCCTTATTCAACAA+TGG 0.546940 5.4:+47255777 MS.gene010315:CDS
TCCACTGTGAGTATCAGATT+GGG 0.550250 5.4:-47253824 None:intergenic
TCTGCCGGAAAGATATTTGT+CGG 0.551480 5.4:+47254066 MS.gene010315:CDS
TGCAGATAAATGGTCATGGC+TGG 0.554287 5.4:-47255857 None:intergenic
GAACTCTGTCATAGGAATGA+AGG 0.555804 5.4:-47255761 None:intergenic
AAGTGAGAATTTAACGGAGA+AGG 0.558251 5.4:+47254137 MS.gene010315:CDS
ATTGGAAACAGATCTATGGT+AGG 0.558884 5.4:+47255922 MS.gene010315:CDS
AAGACATATTATCAGCCTAA+GGG 0.565438 5.4:+47254345 MS.gene010315:CDS
CTGCAGCTGAAGAGGAAGGC+GGG 0.568018 5.4:+47254112 MS.gene010315:CDS
GGAGCTGTGTTTCAATGTCA+GGG 0.572561 5.4:-47253522 None:intergenic
GTCAAGGAAGATATCTCACT+TGG 0.572781 5.4:-47256022 None:intergenic
AGATCTATGGTAGGGTGGAG+TGG 0.573466 5.4:+47255931 MS.gene010315:CDS
TTGGGTGAGAATCTTTACAT+AGG 0.574104 5.4:-47253806 None:intergenic
ATGATGCAAAGAACGGATGG+AGG 0.576993 5.4:-47253559 None:intergenic
GAGATGATGCAAAGAACGGA+TGG 0.581142 5.4:-47253562 None:intergenic
GAAGAACTCCGAGAAAGTCT+AGG 0.583334 5.4:-47255667 None:intergenic
TCAAGTTGTGTATTATCCGA+CGG 0.584356 5.4:+47254203 MS.gene010315:CDS
CCAGACTACGTCTTGAATGT+CGG 0.587404 5.4:-47253932 None:intergenic
GGGTAAATATCAACCTGCCA+AGG 0.589960 5.4:-47255553 None:intergenic
AAAATATGGCAAATATGCGC+CGG 0.590046 5.4:+47253422 None:intergenic
CTGGTTGGTCACAATTTGTG+AGG 0.592410 5.4:+47253998 MS.gene010315:CDS
TAAGAATATGGCATTTGTAG+GGG 0.594369 5.4:-47255598 None:intergenic
TCTTCATCGGGAACCTCAGG+AGG 0.597687 5.4:-47253757 None:intergenic
AGATGAGATGATGCAAAGAA+CGG 0.600160 5.4:-47253566 None:intergenic
AGAATATGGCATTTGTAGGG+GGG 0.601685 5.4:-47255596 None:intergenic
CTCACTCTGGTAACAACCGA+TGG 0.602038 5.4:+47253640 MS.gene010315:CDS
GCGTGGTTCATTGGTCCCAA+AGG 0.605635 5.4:-47255998 None:intergenic
TTGGAAACAGATCTATGGTA+GGG 0.608401 5.4:+47255923 MS.gene010315:CDS
TAAGAATATGGAATTTGTAG+GGG 0.609658 5.4:-47255691 None:intergenic
TACCCTGCAGCTGAAGAGGA+AGG 0.610620 5.4:+47254108 MS.gene010315:CDS
GTTGACGAGACTATGAAGAA+TGG 0.611089 5.4:+47254261 MS.gene010315:CDS
AAAGTCTACTACTTCCCAGA+AGG 0.611295 5.4:+47253475 MS.gene010315:CDS
TGATGCAAAGAACGGATGGA+GGG 0.621507 5.4:-47253558 None:intergenic
AAGAATATGGAATTTGTAGG+GGG 0.621894 5.4:-47255690 None:intergenic
GAAGGCAGTTAAAGATGCTG+TGG 0.623830 5.4:+47254155 MS.gene010315:CDS
ACCTGAAGCATACGCCAGCT+AGG 0.625148 5.4:-47254405 None:intergenic
GCTACCACGTGAAGAAACTC+AGG 0.629119 5.4:+47253968 MS.gene010315:CDS
GGAATTGGCTGGGAAGAACA+TGG 0.633030 5.4:+47254176 MS.gene010315:CDS
ATTTGTCGGAATTCGCAGGA+AGG 0.633253 5.4:+47254080 MS.gene010315:CDS
AGACATATTATCAGCCTAAG+GGG 0.634100 5.4:+47254346 MS.gene010315:CDS
CCAAGAACCAGCTCCTCCTG+AGG 0.640922 5.4:+47253744 MS.gene010315:CDS
AAGATATCTCACTTGGTGCG+TGG 0.645528 5.4:-47256015 None:intergenic
AAGATGCTGTGGAATTGGCT+GGG 0.646479 5.4:+47254166 MS.gene010315:CDS
CAACCTGCCAAGGATTGACG+CGG 0.647296 5.4:-47255543 None:intergenic
GAAACAGATCTATGGTAGGG+TGG 0.650179 5.4:+47255926 MS.gene010315:CDS
ACTCTGGTAACAACCGATGG+TGG 0.653549 5.4:+47253643 MS.gene010315:CDS
CTGATGCAGATAAATGGTCA+TGG 0.654222 5.4:-47255861 None:intergenic
AATAGAGGCGGTGGAATCGG+TGG 0.656000 5.4:-47255715 None:intergenic
TCTTGGTCGGGAAAGTCAGG+AGG 0.656368 5.4:-47253688 None:intergenic
AGAATATGGAATTTGTAGGG+GGG 0.659659 5.4:-47255689 None:intergenic
GAGCTGTGTTTCAATGTCAG+GGG 0.663518 5.4:-47253521 None:intergenic
TAAACCTGAGTTTCTTCACG+TGG 0.666809 5.4:-47253972 None:intergenic
CCCGACCAAGAAGAGAACGA+TGG 0.669874 5.4:+47253700 MS.gene010315:CDS
CCTGCAGCTGAAGAGGAAGG+CGG 0.670142 5.4:+47254111 MS.gene010315:CDS
CATCGGTTGTTACCAGAGTG+AGG 0.674120 5.4:-47253639 None:intergenic
AAATCACACCAATTAGCCGT+CGG 0.674239 5.4:-47254219 None:intergenic
ATCAATTCAATGCATTCACG+AGG 0.696770 5.4:-47253856 None:intergenic
TTCAAACTATTATCGTTACG+AGG 0.697085 5.4:-47255973 None:intergenic
ATAGAAGACTGCAATAGAGG+CGG 0.698613 5.4:-47255727 None:intergenic
ACCCAATCTGATACTCACAG+TGG 0.756201 5.4:+47253823 MS.gene010315:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGATAAAGAGATAAAAAA+GGG - chr5.4:47255484-47255503 None:intergenic 15.0%
!! ATAAGAATATGGAATTTGTA+GGG - chr5.4:47255695-47255714 None:intergenic 20.0%
!! ATATGCACTTGAAATATAAT+AGG - chr5.4:47254440-47254459 None:intergenic 20.0%
!! CTATGATAAAGAGATAAAAA+AGG - chr5.4:47255485-47255504 None:intergenic 20.0%
!! TTAAAATCTTAATGCTAAGT+AGG + chr5.4:47255356-47255375 MS.gene010315:intron 20.0%
!! TTGAAACTAAAATTAGATGA+TGG + chr5.4:47254511-47254530 MS.gene010315:intron 20.0%
!! TTTCAACATCTAAATAAATC+AGG - chr5.4:47254497-47254516 None:intergenic 20.0%
!!! AAGCTATTTTGTAATGATTT+TGG + chr5.4:47254976-47254995 MS.gene010315:intron 20.0%
!!! CTTTTTTATCTCTTTATCAT+AGG + chr5.4:47255483-47255502 MS.gene010315:intron 20.0%
!!! TCAAAAGTTTTGAATGATAA+GGG + chr5.4:47255889-47255908 MS.gene010315:CDS 20.0%
!!! TTCAAAAGTTTTGAATGATA+AGG + chr5.4:47255888-47255907 MS.gene010315:CDS 20.0%
! AAAATATGGATCATGGAAAA+TGG + chr5.4:47254751-47254770 MS.gene010315:intron 25.0%
! AACATGCATTTACACATTTA+AGG - chr5.4:47255419-47255438 None:intergenic 25.0%
! ATAAGAATATGGCATTTGTA+GGG - chr5.4:47255602-47255621 None:intergenic 25.0%
! ATAATAGTTTGAACTCCTTT+GGG + chr5.4:47255983-47256002 MS.gene010315:CDS 25.0%
! GATAAGAATATGGAATTTGT+AGG - chr5.4:47255696-47255715 None:intergenic 25.0%
! TAAGAATATGGAATTTGTAG+GGG - chr5.4:47255694-47255713 None:intergenic 25.0%
! TAGTACTCTAAATGAATGAT+TGG + chr5.4:47254649-47254668 MS.gene010315:intron 25.0%
! TATCAGAATTCAACAATGTT+TGG - chr5.4:47255813-47255832 None:intergenic 25.0%
! TTAACTCATTGAACTTAATG+TGG - chr5.4:47255095-47255114 None:intergenic 25.0%
! TTCTTAGAGAATATCACTAT+TGG - chr5.4:47255283-47255302 None:intergenic 25.0%
!! AAAGTCTAGGAATTTTTGTT+GGG - chr5.4:47255657-47255676 None:intergenic 25.0%
!!! AATCGATTCTAAAATGCAAT+CGG - chr5.4:47255214-47255233 None:intergenic 25.0%
!!! GAATTTCGTTTTGGAAAATA+TGG + chr5.4:47254737-47254756 MS.gene010315:intron 25.0%
AAAAACTCTGAGCAAGTATA+GGG - chr5.4:47255576-47255595 None:intergenic 30.0%
AAGAATATGGAATTTGTAGG+GGG - chr5.4:47255693-47255712 None:intergenic 30.0%
AAGAATCTGATGCAGATAAA+TGG - chr5.4:47255870-47255889 None:intergenic 30.0%
AAGACATATTATCAGCCTAA+GGG + chr5.4:47254345-47254364 MS.gene010315:CDS 30.0%
AATGAGAAATAATGCTCAGA+AGG - chr5.4:47255326-47255345 None:intergenic 30.0%
AATTCCGACAAATATCTTTC+CGG - chr5.4:47254073-47254092 None:intergenic 30.0%
AGCTTCTGCATACATTTAAA+AGG - chr5.4:47254961-47254980 None:intergenic 30.0%
CAAAAATTCCTAGACTTTCT+CGG + chr5.4:47255659-47255678 MS.gene010315:CDS 30.0%
CTCTTTATCATAGGTTAACT+GGG + chr5.4:47255492-47255511 MS.gene010315:intron 30.0%
GATAAGAATATGGCATTTGT+AGG - chr5.4:47255603-47255622 None:intergenic 30.0%
GATAATAGTTTGAACTCCTT+TGG + chr5.4:47255982-47256001 MS.gene010315:CDS 30.0%
GCTTCTGCATACATTTAAAA+GGG - chr5.4:47254960-47254979 None:intergenic 30.0%
TAAGAATATGGCATTTGTAG+GGG - chr5.4:47255601-47255620 None:intergenic 30.0%
TCATTGAACTTAATGTGGTA+TGG - chr5.4:47255090-47255109 None:intergenic 30.0%
TCTCTTTATCATAGGTTAAC+TGG + chr5.4:47255491-47255510 MS.gene010315:intron 30.0%
TGGTTTCTGATGTTAAAGAT+TGG + chr5.4:47254779-47254798 MS.gene010315:intron 30.0%
TTCAAACTATTATCGTTACG+AGG - chr5.4:47255976-47255995 None:intergenic 30.0%
TTTCATTGGAAACAGATCTA+TGG + chr5.4:47255918-47255937 MS.gene010315:CDS 30.0%
! AAGTCTAGGAATTTTTGTTG+GGG - chr5.4:47255656-47255675 None:intergenic 30.0%
! AATTCTGATACTTTTCCAGA+CGG + chr5.4:47255823-47255842 MS.gene010315:CDS 30.0%
! ATTCAACAATGTTTGGTTCA+AGG - chr5.4:47255806-47255825 None:intergenic 30.0%
! CTCTAAATGAATGATTGGTT+TGG + chr5.4:47254654-47254673 MS.gene010315:intron 30.0%
! GAAAGTCTAGGAATTTTTGT+TGG - chr5.4:47255658-47255677 None:intergenic 30.0%
! GTTTTCATAAAAAACTCTGC+CGG + chr5.4:47254051-47254070 MS.gene010315:CDS 30.0%
!! TTCTAAAATGCAATCGGATT+AGG - chr5.4:47255208-47255227 None:intergenic 30.0%
!!! GTTTTGGAAAATATGGATCA+TGG + chr5.4:47254744-47254763 MS.gene010315:intron 30.0%
AAGAAACTCAGGTTTACTTC+TGG + chr5.4:47253979-47253998 MS.gene010315:CDS 35.0%
AAGAATATGGCATTTGTAGG+GGG - chr5.4:47255600-47255619 None:intergenic 35.0%
AAGGATCCCATTGTTGAATA+AGG - chr5.4:47255787-47255806 None:intergenic 35.0%
AAGTGAGAATTTAACGGAGA+AGG + chr5.4:47254137-47254156 MS.gene010315:CDS 35.0%
AGAATATGGAATTTGTAGGG+GGG - chr5.4:47255692-47255711 None:intergenic 35.0%
AGACATATTATCAGCCTAAG+GGG + chr5.4:47254346-47254365 MS.gene010315:CDS 35.0%
AGAGTTCCCTTATTCAACAA+TGG + chr5.4:47255777-47255796 MS.gene010315:CDS 35.0%
AGATGAGATGATGCAAAGAA+CGG - chr5.4:47253569-47253588 None:intergenic 35.0%
AGGATCCCATTGTTGAATAA+GGG - chr5.4:47255786-47255805 None:intergenic 35.0%
AGTTAAAGATGCTGTGGAAT+TGG + chr5.4:47254161-47254180 MS.gene010315:CDS 35.0%
ATCAATTCAATGCATTCACG+AGG - chr5.4:47253859-47253878 None:intergenic 35.0%
ATTGGAAACAGATCTATGGT+AGG + chr5.4:47255922-47255941 MS.gene010315:CDS 35.0%
CAGTTTGGCAAAAACTTCAT+CGG - chr5.4:47253617-47253636 None:intergenic 35.0%
CATAGAGTTGCATAAGAAAC+GGG - chr5.4:47255249-47255268 None:intergenic 35.0%
CGAATAGATTTGTGCACTAT+CGG + chr5.4:47254585-47254604 MS.gene010315:intron 35.0%
CGCATAGTGAAAGTAGAAAT+AGG + chr5.4:47254839-47254858 MS.gene010315:intron 35.0%
CGGGAAAAGTGAGAATTTAA+CGG + chr5.4:47254131-47254150 MS.gene010315:CDS 35.0%
GAAAAACTCTGAGCAAGTAT+AGG - chr5.4:47255577-47255596 None:intergenic 35.0%
GAAGACATATTATCAGCCTA+AGG + chr5.4:47254344-47254363 MS.gene010315:CDS 35.0%
GAGTTCCCTTATTCAACAAT+GGG + chr5.4:47255778-47255797 MS.gene010315:CDS 35.0%
GATAAGGGTGATTGTTTCAT+TGG + chr5.4:47255904-47255923 MS.gene010315:CDS 35.0%
GATCATGGAAAATGGTATAC+TGG + chr5.4:47254759-47254778 MS.gene010315:intron 35.0%
GCATAGAGTTGCATAAGAAA+CGG - chr5.4:47255250-47255269 None:intergenic 35.0%
GGAATTGGAGGATAAGAATA+TGG - chr5.4:47255613-47255632 None:intergenic 35.0%
TAGCACTCATAACAAAGGAA+TGG - chr5.4:47255067-47255086 None:intergenic 35.0%
TATGGTAGCACTCATAACAA+AGG - chr5.4:47255072-47255091 None:intergenic 35.0%
TCTCACTCTCTTGTACTATT+TGG - chr5.4:47254911-47254930 None:intergenic 35.0%
TTGGAAACAGATCTATGGTA+GGG + chr5.4:47255923-47255942 MS.gene010315:CDS 35.0%
TTGGGTGAGAATCTTTACAT+AGG - chr5.4:47253809-47253828 None:intergenic 35.0%
! AGTCTAGGAATTTTTGTTGG+GGG - chr5.4:47255655-47255674 None:intergenic 35.0%
! CTTTGCTTGGAATTTCGTTT+TGG + chr5.4:47254728-47254747 MS.gene010315:intron 35.0%
! GTAGACTTTTGAGCCAATTT+CGG - chr5.4:47253464-47253483 None:intergenic 35.0%
! TAATTGGTGTGATTTTGTGG+TGG + chr5.4:47254227-47254246 MS.gene010315:CDS 35.0%
! TCAAGTTGTGTATTATCCGA+CGG + chr5.4:47254203-47254222 MS.gene010315:CDS 35.0%
!! ATGAATGATTGGTTTGGTCA+TGG + chr5.4:47254660-47254679 MS.gene010315:intron 35.0%
!! GCTTGTTACTTTAGATCAGA+AGG + chr5.4:47254616-47254635 MS.gene010315:intron 35.0%
AAAGTCTACTACTTCCCAGA+AGG + chr5.4:47253475-47253494 MS.gene010315:CDS 40.0%
AAATCACACCAATTAGCCGT+CGG - chr5.4:47254222-47254241 None:intergenic 40.0%
AACTCAGGTTTACTTCTGGT+TGG + chr5.4:47253983-47254002 MS.gene010315:CDS 40.0%
AATTTCGGAATCTTGACAGC+AGG - chr5.4:47253449-47253468 None:intergenic 40.0%
ACGAGACTATGAAGAATGGT+TGG + chr5.4:47254265-47254284 MS.gene010315:CDS 40.0%
AGAATATGGCATTTGTAGGG+GGG - chr5.4:47255599-47255618 None:intergenic 40.0%
AGATATTTGTCGGAATTCGC+AGG + chr5.4:47254076-47254095 MS.gene010315:CDS 40.0%
ATAGAAGACTGCAATAGAGG+CGG - chr5.4:47255730-47255749 None:intergenic 40.0%
ATGAAGAATGGTTGGGAATC+TGG + chr5.4:47254273-47254292 MS.gene010315:CDS 40.0%
ATGTTAGAGATTGTCCCCTT+AGG - chr5.4:47254363-47254382 None:intergenic 40.0%
CATCTCATCTGTTGATCTTC+TGG + chr5.4:47253579-47253598 MS.gene010315:CDS 40.0%
CGAGACTATGAAGAATGGTT+GGG + chr5.4:47254266-47254285 MS.gene010315:CDS 40.0%
CTATCATCGTCTTCTTCATC+GGG - chr5.4:47253772-47253791 None:intergenic 40.0%
CTGATGCAGATAAATGGTCA+TGG - chr5.4:47255864-47255883 None:intergenic 40.0%
GAACTCTGTCATAGGAATGA+AGG - chr5.4:47255764-47255783 None:intergenic 40.0%
GAATAAGGGAACTCTGTCAT+AGG - chr5.4:47255772-47255791 None:intergenic 40.0%
GACATAGAAGACTGCAATAG+AGG - chr5.4:47255733-47255752 None:intergenic 40.0%
GATCTTTCCCAATCTTGACT+TGG + chr5.4:47253876-47253895 MS.gene010315:CDS 40.0%
GCTATCATCGTCTTCTTCAT+CGG - chr5.4:47253773-47253792 None:intergenic 40.0%
GGAATCGGTGGATAAGAATA+TGG - chr5.4:47255706-47255725 None:intergenic 40.0%
GTTGACGAGACTATGAAGAA+TGG + chr5.4:47254261-47254280 MS.gene010315:CDS 40.0%
TAAACCTGAGTTTCTTCACG+TGG - chr5.4:47253975-47253994 None:intergenic 40.0%
TCCACTGTGAGTATCAGATT+GGG - chr5.4:47253827-47253846 None:intergenic 40.0%
TCGACTGAAAACAGTTAGTC+TGG + chr5.4:47254871-47254890 MS.gene010315:intron 40.0%
TCTGCCGGAAAGATATTTGT+CGG + chr5.4:47254066-47254085 MS.gene010315:CDS 40.0%
TGCTGTCAAGATTCCGAAAT+TGG + chr5.4:47253448-47253467 MS.gene010315:CDS 40.0%
TGTATTATCCGACGGCTAAT+TGG + chr5.4:47254211-47254230 MS.gene010315:CDS 40.0%
TGTTTCCATCGTTCTCTTCT+TGG - chr5.4:47253708-47253727 None:intergenic 40.0%
TTCCTTCTAATGTTCCTAGC+TGG + chr5.4:47254391-47254410 MS.gene010315:CDS 40.0%
TTTGGATTCTGCGAGATATC+AGG - chr5.4:47255523-47255542 None:intergenic 40.0%
! AATGGTTGGGAATCTGGAAT+GGG + chr5.4:47254279-47254298 MS.gene010315:CDS 40.0%
! CTGTTTTCAGTCGACAAGTT+AGG - chr5.4:47254865-47254884 None:intergenic 40.0%
! GCGTATGCTTCAGGTATTTT+TGG + chr5.4:47254413-47254432 MS.gene010315:intron 40.0%
! GGCTAATTGGTGTGATTTTG+TGG + chr5.4:47254224-47254243 MS.gene010315:CDS 40.0%
! GTCTAGGAATTTTTGTTGGG+GGG - chr5.4:47255654-47255673 None:intergenic 40.0%
!! TGGTTTGGTCATGGTTAAGT+TGG + chr5.4:47254669-47254688 MS.gene010315:intron 40.0%
AAAGATGCTGTGGAATTGGC+TGG + chr5.4:47254165-47254184 MS.gene010315:CDS 45.0%
AAGATGCTGTGGAATTGGCT+GGG + chr5.4:47254166-47254185 MS.gene010315:CDS 45.0%
ACAGTGACTCAGTCAAAGAC+GGG - chr5.4:47254696-47254715 None:intergenic 45.0%
ACCCAATCTGATACTCACAG+TGG + chr5.4:47253823-47253842 MS.gene010315:CDS 45.0%
AGGAGCTGTGTTTCAATGTC+AGG - chr5.4:47253526-47253545 None:intergenic 45.0%
ATGATGCAAAGAACGGATGG+AGG - chr5.4:47253562-47253581 None:intergenic 45.0%
ATTTGTCGGAATTCGCAGGA+AGG + chr5.4:47254080-47254099 MS.gene010315:CDS 45.0%
CATGTTCCAAGTGACCTTCT+GGG - chr5.4:47253492-47253511 None:intergenic 45.0%
CCAGACTACGTCTTGAATGT+CGG - chr5.4:47253935-47253954 None:intergenic 45.0%
GAAACAGATCTATGGTAGGG+TGG + chr5.4:47255926-47255945 MS.gene010315:CDS 45.0%
GAAGAACTCCGAGAAAGTCT+AGG - chr5.4:47255670-47255689 None:intergenic 45.0%
GAAGGCAGTTAAAGATGCTG+TGG + chr5.4:47254155-47254174 MS.gene010315:CDS 45.0%
GAATGGTTGGGAATCTGGAA+TGG + chr5.4:47254278-47254297 MS.gene010315:CDS 45.0%
GACAGTGACTCAGTCAAAGA+CGG - chr5.4:47254697-47254716 None:intergenic 45.0%
GAGATGATGCAAAGAACGGA+TGG - chr5.4:47253565-47253584 None:intergenic 45.0%
GAGCTGTGTTTCAATGTCAG+GGG - chr5.4:47253524-47253543 None:intergenic 45.0%
GCAAAAACTTCATCGGTGTG+AGG - chr5.4:47253610-47253629 None:intergenic 45.0%
GGAGCTGTGTTTCAATGTCA+GGG - chr5.4:47253525-47253544 None:intergenic 45.0%
GGATCCTCCAAGTCAAGATT+GGG - chr5.4:47253886-47253905 None:intergenic 45.0%
GGGTAAATATCAACCTGCCA+AGG - chr5.4:47255556-47255575 None:intergenic 45.0%
GTCCACTGTGAGTATCAGAT+TGG - chr5.4:47253828-47253847 None:intergenic 45.0%
TCCATCGTTCTCTTCTTGGT+CGG - chr5.4:47253704-47253723 None:intergenic 45.0%
TGATGCAAAGAACGGATGGA+GGG - chr5.4:47253561-47253580 None:intergenic 45.0%
TGCAGATAAATGGTCATGGC+TGG - chr5.4:47255860-47255879 None:intergenic 45.0%
TGGATCCTCCAAGTCAAGAT+TGG - chr5.4:47253887-47253906 None:intergenic 45.0%
! CTGGTTGGTCACAATTTGTG+AGG + chr5.4:47253998-47254017 MS.gene010315:CDS 45.0%
! GGAATTTTTGTTGGGGGGAA+TGG - chr5.4:47255649-47255668 None:intergenic 45.0%
!! CTTTCCCAATCTTGACTTGG+AGG + chr5.4:47253879-47253898 MS.gene010315:CDS 45.0%
AATAGAGGCGGTGGAATTGG+AGG - chr5.4:47255625-47255644 None:intergenic 50.0%
ACTCTGGTAACAACCGATGG+TGG + chr5.4:47253643-47253662 MS.gene010315:CDS 50.0%
AGATCTATGGTAGGGTGGAG+TGG + chr5.4:47255931-47255950 MS.gene010315:CDS 50.0%
ATCCAAACCGCGTCAATCCT+TGG + chr5.4:47255536-47255555 MS.gene010315:CDS 50.0%
ATGGAGGGCGAAGACATAGA+AGG - chr5.4:47253546-47253565 None:intergenic 50.0%
CAGTGACTCAGTCAAAGACG+GGG - chr5.4:47254695-47254714 None:intergenic 50.0%
CCATCGTTCTCTTCTTGGTC+GGG - chr5.4:47253703-47253722 None:intergenic 50.0%
CCGACATTCAAGACGTAGTC+TGG + chr5.4:47253932-47253951 MS.gene010315:CDS 50.0%
CGCCAGCTAGGAACATTAGA+AGG - chr5.4:47254396-47254415 None:intergenic 50.0%
CTACTTCCCAGAAGGTCACT+TGG + chr5.4:47253483-47253502 MS.gene010315:CDS 50.0%
CTCACTCTGGTAACAACCGA+TGG + chr5.4:47253640-47253659 MS.gene010315:CDS 50.0%
GCATGTTCCAAGTGACCTTC+TGG - chr5.4:47253493-47253512 None:intergenic 50.0%
GCTACCACGTGAAGAAACTC+AGG + chr5.4:47253968-47253987 MS.gene010315:CDS 50.0%
GGAATTGGCTGGGAAGAACA+TGG + chr5.4:47254176-47254195 MS.gene010315:CDS 50.0%
GGTAATAGAGGCGGTGGAAT+TGG - chr5.4:47255628-47255647 None:intergenic 50.0%
GTTGGGGGGAATGGTAATAG+AGG - chr5.4:47255640-47255659 None:intergenic 50.0%
TACCAGAGTGAGGAGCAGTT+TGG - chr5.4:47253632-47253651 None:intergenic 50.0%
TATGTACCCTGCAGCTGAAG+AGG + chr5.4:47254104-47254123 MS.gene010315:CDS 50.0%
TCTTCTTCATCGGGAACCTC+AGG - chr5.4:47253763-47253782 None:intergenic 50.0%
TCTTCTTGGTCGGGAAAGTC+AGG - chr5.4:47253694-47253713 None:intergenic 50.0%
TGAGTCACTGTCGCTATGAG+TGG + chr5.4:47254705-47254724 MS.gene010315:intron 50.0%
TGCAATAGAGGCGGTGGAAT+CGG - chr5.4:47255721-47255740 None:intergenic 50.0%
TTGGATTTGGACGCCACCAT+CGG - chr5.4:47253659-47253678 None:intergenic 50.0%
! ACTTTTCCAGACGGCATGCT+GGG + chr5.4:47255832-47255851 MS.gene010315:CDS 50.0%
! CATCGGTTGTTACCAGAGTG+AGG - chr5.4:47253642-47253661 None:intergenic 50.0%
! GAGGAGCTGGTTCTTGGATT+TGG - chr5.4:47253672-47253691 None:intergenic 50.0%
! TACTTTTCCAGACGGCATGC+TGG + chr5.4:47255831-47255850 MS.gene010315:CDS 50.0%
! TCGCTATGAGTGGCTTTGCT+TGG + chr5.4:47254715-47254734 MS.gene010315:intron 50.0%
!! GAGAGCTTCTGAGACTGCAT+TGG - chr5.4:47253907-47253926 None:intergenic 50.0%
!! TCACTTGGTGCGTGGTTCAT+TGG - chr5.4:47256010-47256029 None:intergenic 50.0%
AAACCGCGTCAATCCTTGGC+AGG + chr5.4:47255540-47255559 MS.gene010315:CDS 55.0%
AATAGAGGCGGTGGAATCGG+TGG - chr5.4:47255718-47255737 None:intergenic 55.0%
ACCTGAAGCATACGCCAGCT+AGG - chr5.4:47254408-47254427 None:intergenic 55.0%
CAACCTGCCAAGGATTGACG+CGG - chr5.4:47255546-47255565 None:intergenic 55.0%
CCCGACCAAGAAGAGAACGA+TGG + chr5.4:47253700-47253719 MS.gene010315:CDS 55.0%
CGGAATCTTGACAGCAGGTC+CGG - chr5.4:47253444-47253463 None:intergenic 55.0%
GAAGACTGCAATAGAGGCGG+TGG - chr5.4:47255727-47255746 None:intergenic 55.0%
GCGTGGTTCATTGGTCCCAA+AGG - chr5.4:47256001-47256020 None:intergenic 55.0%
GGGAATGGTAATAGAGGCGG+TGG - chr5.4:47255634-47255653 None:intergenic 55.0%
GGGGGGAATGGTAATAGAGG+CGG - chr5.4:47255637-47255656 None:intergenic 55.0%
TACCCTGCAGCTGAAGAGGA+AGG + chr5.4:47254108-47254127 MS.gene010315:CDS 55.0%
TCCTAGCTGGCGTATGCTTC+AGG + chr5.4:47254404-47254423 MS.gene010315:CDS 55.0%
TCTTCATCGGGAACCTCAGG+AGG - chr5.4:47253760-47253779 None:intergenic 55.0%
TCTTGGTCGGGAAAGTCAGG+AGG - chr5.4:47253691-47253710 None:intergenic 55.0%
TGCCAAACTGCTCCTCACTC+TGG + chr5.4:47253627-47253646 MS.gene010315:CDS 55.0%
TGCCAAGGATTGACGCGGTT+TGG - chr5.4:47255541-47255560 None:intergenic 55.0%
! AGTCAGGAGGAGCTGGTTCT+TGG - chr5.4:47253678-47253697 None:intergenic 55.0%
! GAGGAGCTGGTTCTTGGACT+TGG - chr5.4:47253741-47253760 None:intergenic 55.0%
CCAAGAACCAGCTCCTCCTG+AGG + chr5.4:47253675-47253694 MS.gene010315:CDS 60.0%
CCTGCAGCTGAAGAGGAAGG+CGG + chr5.4:47254111-47254130 MS.gene010315:CDS 60.0%
CGCCTTCCTCTTCAGCTGCA+GGG - chr5.4:47254113-47254132 None:intergenic 60.0%
CTGCAGCTGAAGAGGAAGGC+GGG + chr5.4:47254112-47254131 MS.gene010315:CDS 60.0%
TCGGGAAAGTCAGGAGGAGC+TGG - chr5.4:47253685-47253704 None:intergenic 60.0%
! CCTCAGGAGGAGCTGGTTCT+TGG - chr5.4:47253747-47253766 None:intergenic 60.0%
CCGCCTTCCTCTTCAGCTGC+AGG - chr5.4:47254114-47254133 None:intergenic 65.0%
TCGGGAACCTCAGGAGGAGC+TGG - chr5.4:47253754-47253773 None:intergenic 65.0%
CTGGCTCCCAGCATGCCGTC+TGG - chr5.4:47255841-47255860 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 47253427 47256029 47253427 ID=MS.gene010315
chr5.4 mRNA 47253427 47256029 47253427 ID=MS.gene010315.t1;Parent=MS.gene010315
chr5.4 exon 47253427 47254425 47253427 ID=MS.gene010315.t1.exon1;Parent=MS.gene010315.t1
chr5.4 CDS 47253427 47254425 47253427 ID=cds.MS.gene010315.t1;Parent=MS.gene010315.t1
chr5.4 exon 47255505 47256029 47255505 ID=MS.gene010315.t1.exon2;Parent=MS.gene010315.t1
chr5.4 CDS 47255505 47256029 47255505 ID=cds.MS.gene010315.t1;Parent=MS.gene010315.t1
Gene Sequence

>MS.gene010315

ATGGCAAATATGCGCCGGACCTGCTGTCAAGATTCCGAAATTGGCTCAAAAGTCTACTACTTCCCAGAAGGTCACTTGGAACATGCTTGCTCATCCCCTGACATTGAAACACAGCTCCTTCTATGTCTTCGCCCTCCATCCGTTCTTTGCATCATCTCATCTGTTGATCTTCTGGCAAATCCTCACACCGATGAAGTTTTTGCCAAACTGCTCCTCACTCTGGTAACAACCGATGGTGGCGTCCAAATCCAAGAACCAGCTCCTCCTGACTTTCCCGACCAAGAAGAGAACGATGGAAACAATCTTGTTGTCCAAGTCCAAGAACCAGCTCCTCCTGAGGTTCCCGATGAAGAAGACGATGATAGCAACAATGTTGTTTCCTATGTAAAGATTCTCACCCAATCTGATACTCACAGTGGACTTTTTGTACCTCGTGAATGCATTGAATTGATCTTTCCCAATCTTGACTTGGAGGATCCAATGCAGTCTCAGAAGCTCTCAGTCACCGACATTCAAGACGTAGTCTGGACGTACAAATATAGCTACCACGTGAAGAAACTCAGGTTTACTTCTGGTTGGTCACAATTTGTGAGGAAGAAGAAACTTGCTGCTCTTGATTCTGTTGTTTTCATAAAAAACTCTGCCGGAAAGATATTTGTCGGAATTCGCAGGAAGGCTATGTACCCTGCAGCTGAAGAGGAAGGCGGGAAAAGTGAGAATTTAACGGAGAAGGCAGTTAAAGATGCTGTGGAATTGGCTGGGAAGAACATGGCATTTCAAGTTGTGTATTATCCGACGGCTAATTGGTGTGATTTTGTGGTGGATGCGAGTGTTGTTGACGAGACTATGAAGAATGGTTGGGAATCTGGAATGGGATTCAAGCTTCGTTTGAATGAAGATGCATCTTCAAATTCAAAGAAGACATATTATCAGCCTAAGGGGACAATCTCTAACATGTCTAACGTTCCTTCTAATGTTCCTAGCTGGCGTATGCTTCAGGTATTTTTGGTCCTATTATATTTCAAGTGCATATTGATAAATATAATTTTGTAGTTGATACATTGATGCCTGATTTATTTAGATGTTGAAACTAAAATTAGATGATGGTTCATGAAATAGATGTCTAAAGTTTCGAGATTTAGTATGTTATTACTTTTTCGAATAGATTTGTGCACTATCGGTTATGTATGCTTGTTACTTTAGATCAGAAGGCTCTCATGCATAGTACTCTAAATGAATGATTGGTTTGGTCATGGTTAAGTTGGCCCCGTCTTTGACTGAGTCACTGTCGCTATGAGTGGCTTTGCTTGGAATTTCGTTTTGGAAAATATGGATCATGGAAAATGGTATACTGGTTTCTGATGTTAAAGATTGGCAAAGTAGTCTGTGCTAATCATGCATCAATTTAGTGACGCATAGTGAAAGTAGAAATAGGCCTAACTTGTCGACTGAAAACAGTTAGTCTGGACATTTTGCATGATCCAAATAGTACAAGAGAGTGAGATGATTTAATTCTAACTTGTGTTCTTTCCCTTTTAAATGTATGCAGAAGCTATTTTGTAATGATTTTGGATCACTAAGCTAAGAATGTAGTTAATTTAATTTGCTTTGCTTTGCAAAATAAACTTATAGATGCTCCATTCCTTTGTTATGAGTGCTACCATACCACATTAAGTTCAATGAGTTAAGCAACTTTATACTCTAATTATGAAATTCTTTAATCATTTGAAATAGTTGCAAGAATTTATCTGACTATTTGTTTACATCTGTATACTTTACCTAATCCGATTGCATTTTAGAATCGATTGTTTGTATCACACCCGTTTCTTATGCAACTCTATGCTTAACTTGCTCCAATAGTGATATTCTCTAAGAAATAGTTATGATGTTTAAGAACCTTCTGAGCATTATTTCTCATTGTGAATACTCTTAAAATCTTAATGCTAAGTAGGATCTTGATTTTGACTCATGTTATTAATTGATATGAAGCCTTAAATGTGTAAATGCATGTTATAGTTTATCAAATATTTCAGATGACTGATATTGATTTCTTACCCTTTTTTATCTCTTTATCATAGGTTAACTGGGATGATCCTGATATCTCGCAGAATCCAAACCGCGTCAATCCTTGGCAGGTTGATATTTACCCTATACTTGCTCAGAGTTTTTCCCCCCTACAAATGCCATATTCTTATCCTCCAATTCCACCGCCTCTATTACCATTCCCCCCAACAAAAATTCCTAGACTTTCTCGGAGTTCTTCCCCCCTACAAATTCCATATTCTTATCCACCGATTCCACCGCCTCTATTGCAGTCTTCTATGTCTACTTCGTCCTTCATTCCTATGACAGAGTTCCCTTATTCAACAATGGGATCCTTGAACCAAACATTGTTGAATTCTGATACTTTTCCAGACGGCATGCTGGGAGCCAGCCATGACCATTTATCTGCATCAGATTCTTCAAAAGTTTTGAATGATAAGGGTGATTGTTTCATTGGAAACAGATCTATGGTAGGGTGGAGTGGTACTTTGTGTGACACAGAGCCTCGTAACGATAATAGTTTGAACTCCTTTGGGACCAATGAACCACGCACCAAGTGA
Protein sequence

>MS.gene010315.t1

MANMRRTCCQDSEIGSKVYYFPEGHLEHACSSPDIETQLLLCLRPPSVLCIISSVDLLANPHTDEVFAKLLLTLVTTDGGVQIQEPAPPDFPDQEENDGNNLVVQVQEPAPPEVPDEEDDDSNNVVSYVKILTQSDTHSGLFVPRECIELIFPNLDLEDPMQSQKLSVTDIQDVVWTYKYSYHVKKLRFTSGWSQFVRKKKLAALDSVVFIKNSAGKIFVGIRRKAMYPAAEEEGGKSENLTEKAVKDAVELAGKNMAFQVVYYPTANWCDFVVDASVVDETMKNGWESGMGFKLRLNEDASSNSKKTYYQPKGTISNMSNVPSNVPSWRMLQVNWDDPDISQNPNRVNPWQVDIYPILAQSFSPLQMPYSYPPIPPPLLPFPPTKIPRLSRSSSPLQIPYSYPPIPPPLLQSSMSTSSFIPMTEFPYSTMGSLNQTLLNSDTFPDGMLGASHDHLSASDSSKVLNDKGDCFIGNRSMVGWSGTLCDTEPRNDNSLNSFGTNEPRTK