AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010330.t1 MTR_6g017010 78.056 360 63 2 512 858 413 769 0.0 586
MS.gene010330.t1 MTR_6g017010 73.248 157 22 4 360 513 177 316 4.96e-60 218
MS.gene010330.t1 MTR_6g017010 75.000 44 11 0 307 350 261 304 1.96e-12 71.6
MS.gene010330.t1 MTR_5g085310 80.000 345 53 3 512 842 683 1025 0.0 578
MS.gene010330.t1 MTR_5g085310 82.895 152 25 1 362 513 436 586 9.07e-73 258
MS.gene010330.t1 MTR_5g085310 53.247 77 30 1 307 383 524 594 7.34e-14 76.3
MS.gene010330.t1 MTR_3g107960 65.122 410 78 5 512 858 799 1206 1.98e-173 534
MS.gene010330.t1 MTR_3g107960 80.769 156 27 1 360 512 546 701 2.31e-73 262
MS.gene010330.t1 MTR_3g107960 59.701 67 26 1 297 363 631 696 1.61e-13 75.5
MS.gene010330.t1 MTR_3g101100 63.915 424 74 6 512 858 974 1395 2.29e-169 528
MS.gene010330.t1 MTR_3g101100 75.796 157 35 1 360 513 721 877 1.93e-68 248
MS.gene010330.t1 MTR_3g101100 57.353 68 28 1 296 363 805 871 3.68e-14 77.4
MS.gene010330.t1 MTR_5g017020 70.430 372 35 6 512 858 239 560 2.05e-166 495
MS.gene010330.t1 MTR_5g017020 57.143 189 27 5 331 513 2 142 4.66e-50 186
MS.gene010330.t1 MTR_5g017020 81.787 291 24 4 512 777 239 525 4.34e-160 478
MS.gene010330.t1 MTR_5g017020 57.143 189 27 5 331 513 2 142 4.10e-50 185
MS.gene010330.t1 MTR_6g017000 63.229 223 77 2 512 729 717 939 2.59e-85 293
MS.gene010330.t1 MTR_6g017000 67.974 153 46 2 362 513 470 620 5.22e-58 215
MS.gene010330.t1 MTR_6g017000 48.276 87 38 2 297 383 549 628 4.25e-13 73.9
MS.gene010330.t1 MTR_4g005050 37.500 408 181 6 512 850 1293 1695 1.08e-77 276
MS.gene010330.t1 MTR_4g005050 40.359 223 119 3 291 513 989 1197 9.95e-40 160
MS.gene010330.t1 MTR_0299s0030 77.215 158 32 1 572 729 146 299 7.62e-76 251
MS.gene010330.t1 MTR_6g017130 60.317 189 25 4 653 840 251 390 2.55e-61 213
MS.gene010330.t1 MTR_6g017140 53.500 200 71 2 512 705 270 453 3.27e-61 214
MS.gene010330.t1 MTR_6g017140 58.065 155 51 2 360 513 32 173 3.10e-50 184
MS.gene010330.t1 MTR_6g017140 48.276 87 38 2 297 383 102 181 1.31e-13 74.3
MS.gene010330.t1 MTR_3g062060 55.914 186 32 2 44 179 137 322 8.84e-46 167
MS.gene010330.t1 MTR_5g085317 78.723 94 20 0 515 608 1 94 1.39e-44 157
MS.gene010330.t1 MTR_1g047940 53.143 175 35 5 674 848 71 198 1.49e-42 154
MS.gene010330.t1 MTR_1g047940 62.162 74 23 1 512 585 5 73 3.48e-22 95.9
MS.gene010330.t1 MTR_3g062090 54.802 177 27 2 59 182 1717 1893 4.15e-39 158
MS.gene010330.t1 MTR_3g453030 50.838 179 35 2 57 182 198 376 1.21e-37 146
MS.gene010330.t1 MTR_5g017025 88.462 78 9 0 105 182 53 130 3.07e-36 134
MS.gene010330.t1 MTR_2g450480 51.412 177 33 2 57 180 360 536 9.72e-35 141
MS.gene010330.t1 MTR_6g017135 48.252 143 34 3 461 564 2 143 2.12e-33 125
MS.gene010330.t1 MTR_3g466590 49.444 180 37 3 57 182 408 587 2.77e-32 134
MS.gene010330.t1 MTR_3g466530 58.779 131 38 4 57 182 603 722 1.50e-29 126
MS.gene010330.t1 MTR_3g031750 51.445 173 31 4 56 182 1863 2028 5.51e-29 125
MS.gene010330.t1 MTR_3g466610 46.114 193 51 2 55 194 459 651 1.58e-27 119
MS.gene010330.t1 MTR_1g047930 74.603 63 16 0 451 513 277 339 6.00e-24 105
MS.gene010330.t1 MTR_1g047930 57.895 57 24 0 307 363 277 333 1.61e-11 67.4
MS.gene010330.t1 MTR_0043s0040 62.069 87 26 1 107 186 167 253 1.06e-22 99.0
MS.gene010330.t1 MTR_0043s0040 61.905 84 25 1 106 182 20 103 3.08e-13 70.9
MS.gene010330.t1 MTR_0043s0080 80.769 52 10 0 57 108 454 505 1.21e-16 84.3
MS.gene010330.t1 MTR_0043s0080 80.769 52 10 0 57 108 464 515 1.21e-16 84.3
MS.gene010330.t1 MTR_1142s0010 68.519 54 16 1 57 109 245 298 6.58e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010330.t1 AT1G16710 37.897 409 179 7 512 850 1260 1663 3.96e-80 283
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 1011 1164 4.65e-35 145
MS.gene010330.t1 AT1G16710 37.897 409 179 7 512 850 1289 1692 4.31e-80 283
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 1040 1193 4.86e-35 145
MS.gene010330.t1 AT1G16710 37.897 409 179 7 512 850 1263 1666 4.60e-80 283
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 1014 1167 5.03e-35 145
MS.gene010330.t1 AT1G16710 37.897 409 179 7 512 850 1263 1666 4.60e-80 283
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 1014 1167 5.03e-35 145
MS.gene010330.t1 AT1G16710 37.897 409 179 7 512 850 1263 1666 4.60e-80 283
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 1014 1167 5.03e-35 145
MS.gene010330.t1 AT1G16710 37.897 409 179 7 512 850 1263 1666 4.60e-80 283
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 1014 1167 5.03e-35 145
MS.gene010330.t1 AT1G16710 37.897 409 179 7 512 850 1224 1627 5.08e-80 283
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 975 1128 4.97e-35 145
MS.gene010330.t1 AT1G16710 37.897 409 179 7 512 850 1224 1627 5.08e-80 283
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 975 1128 4.97e-35 145
MS.gene010330.t1 AT1G16710 37.897 409 179 7 512 850 1224 1627 5.08e-80 283
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 975 1128 4.97e-35 145
MS.gene010330.t1 AT1G79000 37.408 409 181 7 512 850 1280 1683 4.82e-78 277
MS.gene010330.t1 AT1G79000 39.462 223 121 3 291 513 976 1184 7.71e-42 166
MS.gene010330.t1 AT1G79000 37.408 409 181 7 512 850 1280 1683 5.02e-78 277
MS.gene010330.t1 AT1G79000 39.462 223 121 3 291 513 976 1184 7.76e-42 166
MS.gene010330.t1 AT1G79000 37.408 409 181 7 512 850 1256 1659 6.75e-78 277
MS.gene010330.t1 AT1G79000 39.462 223 121 3 291 513 952 1160 8.34e-42 166
MS.gene010330.t1 AT3G12980 38.119 404 177 10 512 850 1260 1655 4.00e-72 259
MS.gene010330.t1 AT3G12980 37.220 223 125 3 291 513 957 1164 7.32e-36 147
MS.gene010330.t1 AT3G12980 38.119 404 177 10 512 850 1179 1574 5.41e-72 259
MS.gene010330.t1 AT3G12980 37.220 223 125 3 291 513 876 1083 6.63e-36 147
MS.gene010330.t1 AT1G55970 42.678 239 121 4 512 734 1041 1279 6.93e-52 197
MS.gene010330.t1 AT1G55970 43.871 155 86 1 359 513 806 959 5.12e-34 141
MS.gene010330.t1 AT1G55970 42.678 239 121 4 512 734 1055 1293 7.12e-52 197
MS.gene010330.t1 AT1G55970 43.871 155 86 1 359 513 806 959 5.82e-34 141
MS.gene010330.t1 AT1G67220 32.041 387 190 10 512 830 979 1360 1.64e-50 193
MS.gene010330.t1 AT1G67220 45.806 155 83 1 359 513 730 883 4.44e-35 145
MS.gene010330.t1 AT1G16710 48.387 155 79 1 359 513 1014 1167 3.67e-35 145
MS.gene010330.t1 AT1G16710 42.675 157 82 3 512 660 1263 1419 7.55e-32 134

Find 141 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTCTTCCTACTGAAAA+AGG 0.113508 5.4:-10196499 MS.gene010330:CDS
CCCTGGTAGGCAATATATTT+TGG 0.147750 5.4:+10196116 None:intergenic
AGAGTAGATTGTTTGGAAAT+AGG 0.201852 5.4:-10195698 MS.gene010330:CDS
GAAAGTGAATAAGCAGTTTC+TGG 0.202627 5.4:-10197316 MS.gene010330:CDS
TTGAAGATTCACTGTCAATT+TGG 0.219080 5.4:+10200270 None:intergenic
GAGTAGATTGTTTGGAAATA+GGG 0.235125 5.4:-10195697 MS.gene010330:CDS
CACCAAATGCAAGAAATTTC+AGG 0.236921 5.4:-10196014 MS.gene010330:CDS
GGCAGTGAAGAAGCAGTTTC+TGG 0.255829 5.4:-10198060 MS.gene010330:CDS
TTCCGAGTCTTTGCAATTCC+TGG 0.259668 5.4:+10195206 None:intergenic
CTCCTGAAATTTCTTGCATT+TGG 0.277671 5.4:+10196012 None:intergenic
TCAAGAATTGAAGACTTTGA+TGG 0.290325 5.4:-10200548 MS.gene010330:CDS
GCATTTGAGACGCTTCTTAC+TGG 0.308967 5.4:-10200601 MS.gene010330:CDS
GCATTTGAGACGCTTCTTAC+TGG 0.308967 5.4:-10200799 MS.gene010330:CDS
TTGCATCTCCAAAGTCTCTT+TGG 0.316344 5.4:+10196306 None:intergenic
TGGTCACTTAGCACTGTTCT+TGG 0.319724 5.4:+10197683 None:intergenic
TTGCCGTAGAGCTGAAGTTT+TGG 0.336259 5.4:-10194948 MS.gene010330:CDS
TGAAGATTCACTGTCAATTT+GGG 0.354045 5.4:+10200271 None:intergenic
CTTCTGTTTAAGCTGATTGA+AGG 0.363488 5.4:-10197104 MS.gene010330:CDS
TTCTTGAAGTAAAGTTCTAA+TGG 0.364202 5.4:+10200564 None:intergenic
AGTGTCAAATAGAACATTAA+AGG 0.370702 5.4:-10196350 MS.gene010330:CDS
TGACGACAGTGATGGATCTA+AGG 0.378087 5.4:-10202294 MS.gene010330:CDS
ACTTTCTGATTGTGCTTCCT+TGG 0.380253 5.4:-10200097 MS.gene010330:CDS
GTCCTTGAGAGTAGATTGTT+TGG 0.389989 5.4:-10195705 MS.gene010330:CDS
GCATCAGAATTAGCCGCCTT+TGG 0.391601 5.4:+10197716 None:intergenic
CAGCAGTCTCCTTCAAAGTA+TGG 0.394018 5.4:+10196448 None:intergenic
AAGTGTGTTGATCATAACTA+TGG 0.394998 5.4:+10195887 None:intergenic
AAGAGAAATCAACTTCAATC+TGG 0.403240 5.4:-10199957 MS.gene010330:CDS
ATGCATCTCAATGCCGCCGC+CGG 0.412185 5.4:-10195368 MS.gene010330:CDS
TCGCATTAGGATTGTGTAGA+TGG 0.417229 5.4:+10195589 None:intergenic
CAGCATTACTTGCCCGGCGG+CGG 0.424812 5.4:+10195355 None:intergenic
CAAAGTCTCTTTGGAAGGCT+TGG 0.425033 5.4:+10196315 None:intergenic
TTTCGTTTGCCATACTTTGA+AGG 0.426623 5.4:-10196457 MS.gene010330:CDS
TTTCAGTAGGAAGAAAGAAA+TGG 0.429433 5.4:+10196503 None:intergenic
TCATGCAAGAGAGAGAAAAC+TGG 0.430015 5.4:-10197634 MS.gene010330:CDS
TCAGCAGGGTAATTCTCCTC+TGG 0.435068 5.4:+10197285 None:intergenic
TGCATCTCAATGCCGCCGCC+GGG 0.437980 5.4:-10195367 MS.gene010330:CDS
CAATGACAACATTATCAAAC+AGG 0.440162 5.4:+10200057 None:intergenic
CTGATCTTATGAACAAGCTT+AGG 0.447135 5.4:-10200313 MS.gene010330:CDS
ATCATTATGTTTAGTGTTGG+AGG 0.449050 5.4:+10195731 None:intergenic
AATCTATAAAAGAATTCAGC+AGG 0.450271 5.4:+10198014 None:intergenic
TTCTTGATTTGAGAACAGTT+TGG 0.456469 5.4:+10195318 None:intergenic
CAGCTTTCGAATCAAGAAGT+TGG 0.459447 5.4:+10200333 None:intergenic
AGAATTAGCCGCCTTTGGTA+AGG 0.464266 5.4:+10197721 None:intergenic
TCAGCAGGGTAATTCTCATC+TGG 0.465105 5.4:+10198029 None:intergenic
TGCCGTGAAGTGATAGTGTC+TGG 0.465307 5.4:-10196052 MS.gene010330:CDS
CAAGCCTTCCAAAGAGACTT+TGG 0.467808 5.4:-10196314 MS.gene010330:CDS
CAAACTGAAACTGAGACTTC+TGG 0.468204 5.4:+10195655 None:intergenic
AATATCATTATGTTTAGTGT+TGG 0.474871 5.4:+10195728 None:intergenic
AGGCAGAGTAGTATTTAGTC+AGG 0.479801 5.4:-10198921 MS.gene010330:CDS
GATCTATAAGAGAATTCAGC+AGG 0.480043 5.4:+10197270 None:intergenic
TGAGGTGCATACACTTTGTC+AGG 0.483949 5.4:-10195854 MS.gene010330:intron
GCCAAAATATATTGCCTACC+AGG 0.484399 5.4:-10196117 MS.gene010330:CDS
ATGGCTCAGCATTACTTGCC+CGG 0.490163 5.4:+10195349 None:intergenic
TATGACAGACAACAATTCAA+TGG 0.493279 5.4:-10198843 MS.gene010330:CDS
AAAGAAATGGTCATGTATGT+TGG 0.496652 5.4:+10196516 None:intergenic
TCCCAGACACTATCACTTCA+CGG 0.498199 5.4:+10196050 None:intergenic
ACCGAAGAAAATATTGTCGT+TGG 0.500600 5.4:-10196544 MS.gene010330:CDS
AGTGGGATGAATGCAATAGA+TGG 0.504930 5.4:-10197853 MS.gene010330:CDS
CATTATGTTTAGTGTTGGAG+GGG 0.505283 5.4:+10195733 None:intergenic
GTGCAAAATTCAATTAAATG+AGG 0.507289 5.4:-10195274 MS.gene010330:CDS
GATCAACACACTTCAAAGAA+TGG 0.512393 5.4:-10195877 MS.gene010330:CDS
CTCCAGGAATTGCAAAGACT+CGG 0.514621 5.4:-10195208 MS.gene010330:CDS
ATCTCCAAAGTCTCTTTGGA+AGG 0.515718 5.4:+10196310 None:intergenic
TCATTATGTTTAGTGTTGGA+GGG 0.517970 5.4:+10195732 None:intergenic
TGTTCAGAAGGAGAAGTTGA+TGG 0.521325 5.4:-10200156 MS.gene010330:CDS
AATCATCATAGAGGAATACT+TGG 0.522130 5.4:+10195617 None:intergenic
GGCGGCTAATTCTGATGCTA+AGG 0.522133 5.4:-10197711 MS.gene010330:CDS
CGAAAAGTCTTATCTCTAAG+TGG 0.523031 5.4:+10197663 None:intergenic
CAGCAGGGTAATTCTCCTCT+GGG 0.526093 5.4:+10197286 None:intergenic
TGCAAAATTCAATTAAATGA+GGG 0.527070 5.4:-10195273 MS.gene010330:CDS
GCACTGATTTCTGTTCTACA+TGG 0.527594 5.4:+10194981 None:intergenic
AGAAATTTCAGGAGTGTGAA+AGG 0.528641 5.4:-10196003 MS.gene010330:intron
CTGCTGGTGTGGTAATGCTA+TGG 0.533073 5.4:-10196431 MS.gene010330:CDS
TTTCCAAAACTTCAGCTCTA+CGG 0.533760 5.4:+10194945 None:intergenic
GTTATCTGTTGACAACCAAC+TGG 0.534094 5.4:-10198081 MS.gene010330:CDS
GAATTAGCCGCCTTTGGTAA+GGG 0.534897 5.4:+10197722 None:intergenic
AGACATTAACAAACAGATTG+AGG 0.535846 5.4:-10200638 MS.gene010330:CDS
AGACATTAACAAACAGATTG+AGG 0.538285 5.4:-10200836 MS.gene010330:CDS
CATGGACTGAATCAGCATCA+AGG 0.538652 5.4:+10199983 None:intergenic
ACACTTCTCAACAAGTACTT+AGG 0.539088 5.4:-10200364 MS.gene010330:CDS
AATGTAGAATACCACGCTGC+AGG 0.540083 5.4:-10195185 MS.gene010330:intron
TTATACTAACTTCTCACTCC+AGG 0.540435 5.4:-10195224 MS.gene010330:CDS
TGGATCTAAGGGTCGGAATG+AGG 0.544069 5.4:-10202282 MS.gene010330:CDS
TTGATGGCTAATGCACTGAA+TGG 0.547833 5.4:-10200140 MS.gene010330:CDS
AGAGTAGTATTTAGTCAGGA+GGG 0.549852 5.4:-10198917 MS.gene010330:CDS
GAACAAATGTGATGTTCAGA+AGG 0.551746 5.4:-10200168 MS.gene010330:CDS
ACAAACTCCTTCACATCAGA+AGG 0.559927 5.4:-10200013 MS.gene010330:CDS
GAAAGCGATGGAGCAATCGT+TGG 0.563087 5.4:-10194926 MS.gene010330:CDS
CATGCAAGAGAGAGAAAACT+GGG 0.565706 5.4:-10197633 MS.gene010330:CDS
TGATGGCTAATGCACTGAAT+GGG 0.574148 5.4:-10200139 MS.gene010330:CDS
TTCCAAACAATCTACTCTCA+AGG 0.576903 5.4:+10195703 None:intergenic
CTCAAAAGATATCAGAGGAG+GGG 0.578093 5.4:-10198886 MS.gene010330:CDS
AGCGTCTCAAATGCAAGAAG+CGG 0.579572 5.4:+10200610 None:intergenic
AAATAGAACATTAAAGGACA+TGG 0.581441 5.4:-10196344 MS.gene010330:CDS
GGCAGCGACGACAGCGAGGG+TGG 0.581889 5.4:-10202317 MS.gene010330:CDS
CAAACGAAAAGCTGTCACCT+TGG 0.585482 5.4:+10196471 None:intergenic
ACTCATACCTTCTGATGTGA+AGG 0.585878 5.4:+10200006 None:intergenic
ACTGCTTCTTCACTGCCAGT+TGG 0.587332 5.4:+10198066 None:intergenic
GAACAGTTTGGATGAGAGCA+TGG 0.587828 5.4:+10195330 None:intergenic
TGATAGTGTCTGGGAAGCGA+TGG 0.590031 5.4:-10196042 MS.gene010330:CDS
CCAAAATATATTGCCTACCA+GGG 0.595401 5.4:-10196116 MS.gene010330:CDS
AGTGACGACGATGGTGGTGG+TGG 0.597604 5.4:-10202250 MS.gene010330:intron
CATACTTTGAAGGAGACTGC+TGG 0.599995 5.4:-10196447 MS.gene010330:CDS
GACGACAGTGATGGATCTAA+GGG 0.600417 5.4:-10202293 MS.gene010330:CDS
CAGAGTAGTATTTAGTCAGG+AGG 0.600694 5.4:-10198918 MS.gene010330:CDS
GATGTTGAACAGGACATACA+AGG 0.607215 5.4:-10198941 MS.gene010330:intron
TGCTCAAAAGATATCAGAGG+AGG 0.607660 5.4:-10198888 MS.gene010330:CDS
GGGAAAGATCTTGAAATACA+TGG 0.610038 5.4:-10198866 MS.gene010330:CDS
AATATTGCAAGTGAGCTATG+AGG 0.614269 5.4:+10196086 None:intergenic
GCTCAAAAGATATCAGAGGA+GGG 0.621747 5.4:-10198887 MS.gene010330:CDS
ACTCGTGGTTAGATACAAGT+TGG 0.623528 5.4:-10199934 MS.gene010330:CDS
TCAACTTCAATCTGGACTCG+TGG 0.629157 5.4:-10199949 MS.gene010330:CDS
AATAGAACATTAAAGGACAT+GGG 0.630593 5.4:-10196343 MS.gene010330:CDS
GCATGTTCCCTTACCAAAGG+CGG 0.630753 5.4:-10197729 MS.gene010330:CDS
AATTCAATGGAAATTGACAT+CGG 0.636320 5.4:-10198830 MS.gene010330:CDS
TTTGAAGGAGACTGCTGGTG+TGG 0.637044 5.4:-10196442 MS.gene010330:CDS
GATGGCAGCGACGACAGCGA+GGG 0.639022 5.4:-10202320 MS.gene010330:CDS
GCCGTGAAGTGATAGTGTCT+GGG 0.641094 5.4:-10196051 MS.gene010330:CDS
GTTAGTGACGACGATGGTGG+TGG 0.644571 5.4:-10202253 MS.gene010330:CDS
GAGTAGTATTTAGTCAGGAG+GGG 0.652349 5.4:-10198916 MS.gene010330:CDS
GGTTAGATACAAGTTGGTCA+AGG 0.652859 5.4:-10199928 MS.gene010330:intron
AATGCATGTTCCCTTACCAA+AGG 0.654704 5.4:-10197732 MS.gene010330:CDS
ACAGTGATGGATCTAAGGGT+CGG 0.659796 5.4:-10202289 MS.gene010330:CDS
ATCTATAAAAGAATTCAGCA+GGG 0.660921 5.4:+10198015 None:intergenic
GAGGGTGGTGACGACAGTGA+TGG 0.665661 5.4:-10202302 MS.gene010330:CDS
AGGGAATGAGAATCTTGATG+AGG 0.666581 5.4:-10197600 MS.gene010330:intron
TAGAGGAATACTTGGCACGA+CGG 0.668087 5.4:+10195625 None:intergenic
GGTTAGATACAAGATGGTCA+AGG 0.669020 5.4:+10200512 None:intergenic
ATCTATAAGAGAATTCAGCA+GGG 0.680316 5.4:+10197271 None:intergenic
AGTTGCTCAAAAGATATCAG+AGG 0.685664 5.4:-10198891 MS.gene010330:CDS
ACGGTTAGTGACGACGATGG+TGG 0.698057 5.4:-10202256 MS.gene010330:CDS
GGAGGGGATATCATCAACAA+CGG 0.699125 5.4:+10195749 None:intergenic
CATTAACAAACAGATTGAGG+AGG 0.701927 5.4:-10200833 MS.gene010330:CDS
ACACACTTCAAAGAATGGTG+AGG 0.703040 5.4:-10195872 MS.gene010330:CDS
GCTCAGCATTACTTGCCCGG+CGG 0.716635 5.4:+10195352 None:intergenic
GGATGGCAGCGACGACAGCG+AGG 0.724417 5.4:-10202321 MS.gene010330:CDS
ACGACGGTTAGTGACGACGA+TGG 0.724974 5.4:-10202259 MS.gene010330:CDS
ATGGTAGAGAATCATCATAG+AGG 0.733865 5.4:+10195608 None:intergenic
ATTGTAACACACCTGCAGCG+TGG 0.738381 5.4:+10195174 None:intergenic
GTTTCTGGACATCATCCCAG+AGG 0.751856 5.4:-10197301 MS.gene010330:CDS
AAGGGTCGGAATGAGGACGA+CGG 0.752142 5.4:-10202275 MS.gene010330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.4 gene 10194919 10202341 10194919 ID=MS.gene010330
chr5.4 mRNA 10194919 10202341 10194919 ID=MS.gene010330.t1;Parent=MS.gene010330
chr5.4 exon 10202251 10202341 10202251 ID=MS.gene010330.t1.exon1;Parent=MS.gene010330.t1
chr5.4 CDS 10202251 10202341 10202251 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10200791 10200865 10200791 ID=MS.gene010330.t1.exon2;Parent=MS.gene010330.t1
chr5.4 CDS 10200791 10200865 10200791 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10200522 10200667 10200522 ID=MS.gene010330.t1.exon3;Parent=MS.gene010330.t1
chr5.4 CDS 10200522 10200667 10200522 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10199929 10200387 10199929 ID=MS.gene010330.t1.exon4;Parent=MS.gene010330.t1
chr5.4 CDS 10199929 10200387 10199929 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10198805 10198951 10198805 ID=MS.gene010330.t1.exon5;Parent=MS.gene010330.t1
chr5.4 CDS 10198805 10198951 10198805 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10197980 10198138 10197980 ID=MS.gene010330.t1.exon6;Parent=MS.gene010330.t1
chr5.4 CDS 10197980 10198138 10197980 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10197601 10197873 10197601 ID=MS.gene010330.t1.exon7;Parent=MS.gene010330.t1
chr5.4 CDS 10197601 10197873 10197601 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10197266 10197394 10197266 ID=MS.gene010330.t1.exon8;Parent=MS.gene010330.t1
chr5.4 CDS 10197266 10197394 10197266 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10197080 10197132 10197080 ID=MS.gene010330.t1.exon9;Parent=MS.gene010330.t1
chr5.4 CDS 10197080 10197132 10197080 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10196303 10196591 10196303 ID=MS.gene010330.t1.exon10;Parent=MS.gene010330.t1
chr5.4 CDS 10196303 10196591 10196303 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10196004 10196143 10196004 ID=MS.gene010330.t1.exon11;Parent=MS.gene010330.t1
chr5.4 CDS 10196004 10196143 10196004 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10195855 10195912 10195855 ID=MS.gene010330.t1.exon12;Parent=MS.gene010330.t1
chr5.4 CDS 10195855 10195912 10195855 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10195545 10195772 10195545 ID=MS.gene010330.t1.exon13;Parent=MS.gene010330.t1
chr5.4 CDS 10195545 10195772 10195545 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10195186 10195418 10195186 ID=MS.gene010330.t1.exon14;Parent=MS.gene010330.t1
chr5.4 CDS 10195186 10195418 10195186 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
chr5.4 exon 10194919 10195015 10194919 ID=MS.gene010330.t1.exon15;Parent=MS.gene010330.t1
chr5.4 CDS 10194919 10195015 10194919 ID=cds.MS.gene010330.t1;Parent=MS.gene010330.t1
Gene Sequence

>MS.gene010330

ATGGCAGCGACGACAGCGAGGGTGGTGACGACAGTGATGGATCTAAGGGTCGGAATGAGGACGACGGTTAGTGACGACGATGGTGGTGGTGGTAAGATTTGAGGTTGAAAACGGCGGAGGGCGGAGGACGGTGGTTGTGGTGGAGATGAACGGGTGGTTGGATAAGTGAGAAAGGTTGAAGAGAACTGAATGCTGAATGAGAGAGACAATTTTTTTGAAAGAGTAGACTTTCTTTTTTTTGTTTGTTGAGCTGGCTTTTTTTATACTTCATTGTGACAAGTGGGGGGTGTAGGAGAGGGGTACACCACGTAAGGGTGTTTACCTATCAGCTCTCAGACTTTATTACTTTGCCATAAACTATTTTAATCAAAAGAATTGATTGTAAGATTACTTTTGATTAGAAATTTAATCATTTGAAATGAAAGCTATGTATAAATACGTACTAATTACTTGACTTATCAGATTACTTCAGAAGAAATAATTATCAGATTAATTAAGAAAACAAGAACAACAATTGTGTCAACCTCAGAGCGTCAACACCAAGTTGCTTGTAACTTGTATGTCTCTCTTCCTCTTTCTCTATCTCTAATTTTCTCTTTTTCCCAATTCTTAAACCCTAATTTTAATTTTATTCAATTCAAATTTTGTGGGTTTTCTTTTTAAATCAAGTGATTATTCAATCCACAGTTTTGTAACGTTGCTATGGTTATGAATTGATTATAATAACTTCTTTCTTGCTGTTAATTTCTGTTTTCTTTTATAAAATTAATTCGTTTTTTTCTTCTTCTTTGAATTAATGAATTTGAGTCTTTGAATGTTGATTAATAATAATGATGATGATAAAGGGTTCCAAGAATCTTTGACACTTTTTTAATTTTGGATAAAAAAAGGAAAAAGAAAAAAGAAAGGAAGATTTTAATTGTGACAATGGGAGTGTTGTTAGATACAGTACACTTTTAGTACAAAATACTGCGGTGCTATAACATTGTTGCGTAGCGGAATTCGGTATATAGATGATTGTTAAAATTCGGCCACGATTTAGTGGTATAGCGCTGTTATAGCTGTTATTCAACAACATTGGTTGTTACTTTTTAATGTTACGATATAACTTTTCTGGTCAAGTGAAATTCAAATAGGGGCTCTGTTATTTTGATTAGATTTGTCTTCTTTGATTTTCTTTCTCATGTTCATCCTGAATTGTTAATTAATTTGATCTTATTTGTTAATTAATTTGAATTGCTAGGAAGTTGGTGATGAAAAAATATGCATACCCTCTTCTTCAAATGTCAATATAAAGCTTCCTGCCACCAAATATGTTGAAATTGGAGACTCCCAGGAAACTATTTGTGAGTTTTATTTTTCTTCTCTAAATTTTAAGGTTTCTCCTACCACTTTGCATTTAGTTTCTTAACTTAAATTATAATTCTTCCATGAGAGTAATAAGAAGATTATAAGTAAATAAATGATTTATTGTAGGAATAGAAGACATTAACAAACAGATTGAGGAGGACTCGCTTCTTGCATTTGAGACGCTTCTTACTGGAGTGCAAAGTTATTCCATTAGAACTTTACTTCAAGAATTGAAGACTTTGATGGACTCATCATCAGACCAAAATTATAATTCTTATATTAGACTAATAAGAAGATTATAAGTGAATAAATGATTGTTTGTAGCAATGGAAGACATTAACAAACAGATTGAGGATGATCCGCTTCTTGCATTTGAGACGCTTCTTACTGGAGTGCAAAGTTATTCCATTAGAACTTTACTTCAAGAATTGAAGACTTTGATGGACTCATCATCAGACCTTGACCATCTTGTATCTAACCACGAGTCCAAACTGAAGTTGATTTCTCTTTTTCATGGACTGAATCAACATCGAGGACTCTTACCTTCTGATGTGAAGGAATTTGTTGAGATAGTGCAAAATTTCTTCAATGTCAACATAATCAGGACACTTCTCAACAAGTACTTAGGAAGCACAACCAACTTCTTGATTCGAAAGCTGATCTTATGAACAAGCTTAGGAGTGCAAAGAGTACACAAACCCAAATTGACAGTGAATCTTCAACTGCAAATGCGCAGATACACAAACTCTCTTTACAAATTGACGATCTTGTGAAACAAAGAGATGATTTGAAATCAGCAGTGAACAAATGTGATGTTCAGAAGGAGAAGTTGATGGCTAATGCACTGAATGGGCACAACAAAGTAAAGAGTTACTTTCTGATTGTGCTTCCTTGGTACTTTTTGAGAAGTGTCCTGTTTGATAATGTTGTCATTGAAGAAGTTCTGCACTATCTCAACAAACTCCTTCACATCAGAAGGTATGAGTCCTTGATGCTGATTCAGTCCATGAAAAAGAGAAATCAACTTCAATCTGGACTCGTGGTTAGATACAAGTTGGTCAAGGTCTGATGATGAGTCCATCAAAGTCTTCAATTCTTGAAGTAAAGTTCTAATGGAATAACTTTGCACTTCAGTAAGAAGCGTCTCAAATGCAAGAAGCGGGTCCTCCTCAATCTGTTTGTTAATGTCTTCCATTGCTACAAACAATCATTTATTCACTTATAATCTTCTTATTACCCTCGTGGAAGAATTATAATTTAAGTTAAGAAACTAAACGCAAAGTGGGAGGAGAAACCTTAAAATTCAGAGAAGAAAAATGAAACTCACCAATAGTTTCCTGGGAGTCTCCAATTTCAACATATTTGGTGGCAGGAAGCTTTATATTGACGTTTGAAGAAGAGGGTATGCATATTTTTTCTTCACTTTCTTTGTTTATTTATTCAAATAGTGAATCATGGACTTTTCTACTTAAACATTTGATCTTTGATTATATGATTTCTGATTGCTGTTCACATTGGTATTTATATATGCACACTTAATTGTGAGCTTGTTTAAATTTATGTTAATCATGTTTTCCCTCTTTATTAGTTTATGGCACATCAAGAACATTGTTGTAATTTCTCTAATTTATATTTGTTGATTGTTTCTTGTGGCTTCAGAATTTAAATAGCATCTGATAAAGCGAGAAGATGCTTACTAAGGGTTGTCATATTGGTGCGAATAATTGGAGCGAAGTACCAAATCATTCTTCATCAGTTATCTCAAACATTCCACGAAAGAGATGTTTTGAAGAATTAAGCTATCAAATAGGATTCTCCACAGACTGGCGGTATCATTCTGAAATTGTGTACAACCGTAATTGGATAAGAAGAAAAATGTAAGTGAAAGTGATTTAGTTTTCAAAGAAATTATTCAGTTTTGAAATCATTTAGAACGAGAAATTTGCACCTATTGTGGAAATATTGCTATTTACCGATGCTGCTTCAAAGGTTTGTTTCTTGAATTGTGTCTGAGCTATTATTGATGATGATGTTGAACAGGACATACAAGGCAGAGTAGTATTTAGTCAGGAGGGGACAGTTGCTCAAAAGATATCAGAGGAGGGGAAAGATCTTGAAATACATGGTATGACAGACAACAATTCAATGGAAATTGACATCGGAGCGAATGACAATATGCATGCCCTCGTAAGCCAGCTTCCAATTCCTATTGATGTAGAGGACAAACAAGACGGAATTTGATTCAACCAGGAGGGGACAGATTCTCAAGAAATTCACTGAGCCAAAAGCTAATCAACAGAAGCAAACCACATCCTCAAATAAAATAAATGATGTTGTTTCCTTGATTGATCTGTCCACCCATGATCAAATAACCAAACATATTTGAAGTCTGAAAAAGCAAATCCGTTCAGGTTAGTACACCACCTTCCTGTGTTTTTAAATACATTGATCTGTTTAGGCATCGGTCTTCCTCTTCTTTTAAGCATTTGATTTATGATAATTCATGTTTGTAAGCATTTGGTTTTATGATAATTCATGTTGTCTAAGCATTTGGTTTATAACATAGGAATTTTAATGCATTGTGAAGTTGTGTCTTTCTAAGCCATATACAAGTTGACACTTTTTTTTTTAACAGAGTTCAACTGTGGGCGACAACAACACATGTCAATAATTGCTGTCAGTAATAATTAGTTAGATAATTGATTCCTTTTATATAACTATCAGTCCGATCAATAGTAGTCTGAAATTTATCAGTATTATTGATATTTTTCAGTCCCTTCCTAGTATGTTATTTAAAAAGAAATTGAATATTCATTATTGTCTGATTTGTCATGCATGACTCCAAAACTGTAGGTTGTAGTTGCAGAAAGTATCTCTATTAGAGAAGTGTTATCTGTTGACAACCAACTGGCAGTGAAGAAGCAGTTTCTGGACATCATTCCAGATGAGAATTACCCTGCTGAATTCTTTTATAGATTAAGAGTAAGTTTAAAGTTTAAAGTGTTACACCAAGTTTGATGTCTACTCTATTATGTCTGTCTTTAATATCAATTACTATATAATTCAGCTTGATAGAGAGAAATTTAAACTAACTCTTTATGACTGTTTTTTCTTCTAGTGGGATGAATGCAATAGATGGCAGCATCAAATTTGTGCACTCTACAACAAGAAAGCAGATTTAGACTGCAGTGCTGAATATATATGTCATGTATACCACCAAAAAGAAATTGAAAATGGAATGCATGTTCCCTTACCAAAGGCGGCTAATTCTGATGCTAAGGACTTTCCAAGAACAGTGCTAAGTGACCACTTAGAGATAAGACTTTTCGAGCGTCTCATGCAAGAGAGAGAAAACTGGGAAAAAGTTGAAGGGAATGAGAATCTTGATGAGGTAATACTGAGAAATCTGCATGTATGCACATAAACTTCAAATATCTTATTTTCTTATTCTTATGCAAGCATGTAAATTGAAAATAGTAATAAATTAGTATCCTGCCATGTTCCCTTAAAATTATTGATCTTTTTCTTCGAAATATATATTGATTTGAAAATTCTAGATTATTTACTTACGTGTCTCGAATTACTGCAAAATTGCAGGTTTTAGCAGCAGAAAGTCTTTCAATTAGAAAAGTGCTATCTATCAACAAGCAATTGAAAGTGAATAAGCAGTTTCTGGACATCATCCCAGAGGAGAATTACCCTGCTGAATTCTCTTATAGATCAAGAGTAAGTTCAGTGGTTTATCAATGAATAAATGGATATACCACCAGTTGCTTCTTCTGAACTAAATGTCTCTTGATGTATACTCTAACATGTCTGAATTATTCCGAACATTTAATAGAAGTTCTCTTGATTTCAGGTTATTCTTCTGTTTAAGCTGATTGAAGGAGCAGATGTATGCATTTTTGGAATGTACGTCCAGGAATTTGACTCTGAATGCAGCAGTCCCAATCAGCGCTTGTGTATGCATATCATATCTTGATTCCGTCAAGTATTTTAGGCCTGAAAGAAGGACAAAGTGTGGAGAAGCCCTTCGTACCTTCGTTTACCATGAGATATTGGTATTTTTCTTTTAAACTCTAATTCTATGCCAATAAAACTAATAAAATGAACACTTAATCTTTCCATTCTCTCTTTTTATGCTTTATTTTTATTTCTTATCATCAGATTAGATACCTTGATTTTTGTAACAAAAGGGGTTTCTCAACTTGCTACATATGGACCTGTGCTCCTTCAAAGAAAGGGAATGATTATATACTATATTGCCATCCCGAAGAGCAAAAGACTCCGAAGAATGATAAGCTACGCCGCTGGTCTGTCTTTTGTTTCTCTTTATGTGTCTGTGTGTGTTGAATTTTCATGAGCTATTTTCCTTATTTTTATATTGTTTGTTTATGCAGGTATCTTTTGATGCTAAAAAAAGCTACCGAAGAAAATATTGTCGTTGGTTTAACCAACATACATGACCATTTCTTTCTTCCTACTGAAAAAGGGAACTCCAAGGTGACAGCTTTTCGTTTGCCATACTTTGAAGGAGACTGCTGGTGTGGTAATGCTATGGAAGTAGCCAAGACCTTCGAAAAAGAGAGTGTAGGAGACTATGAGAAGTTGTTGAAACAAGTGTCAAATAGAACATTAAAGGACATGGGACATGCCAAGCCTTCCAAAGAGACTTTGGAGATGCAAAAAGTATGTCTATCTTGTCATCCAATGGGATATATCCTTTTATACTTGAGTAAATGAAATGTTGCTATGTTCCTATTATAGACTAGTTTAAGTTATTTTCATAATATGTACATGTGTAATCTGATCTTTCTTCCTTTTCTTTTTCCCCCTAACTTCTATTAGGTTGGCCAAAATATATTGCCTACCAGGGAAAACTTCCTCATAGCTCACTTGCAATATTCATGCTTGCACTGCCGTGAAGTGATAGTGTCTGGGAAGCGATGGTTTTACACCAAATGCAAGAAATTTCAGGAGTGTGAAAGGTATGATAACTTGAATACACATTGAACTGTTTTTAGCATTGAATGAAATAAAAAGGGCTTTTGTCATTGACTTTCACTTTAAGTATTTCAGATGCCATAGTTATGATCAACACACTTCAAAGAATGGTGAGGTGCATACACTTTGTCAGGTAACAACCTAAACCTGAAGTTTTTCATTTCATATGATTGTTTCATCATTAGTGATTTCGTATAATTGTGTAACGTTTTCAGGCCGTTGTTGATGATATCCCCTCCAACACTAAACATAATGATATTGTCCTTGAGAGTAGATTGTTTGGAAATAGGGACAACTTTTTGATCTTCTGCCAGAAGTCTCAGTTTCAGTTTGACACACTCCGTCGTGCCAAGTATTCCTCTATGATGATTCTCTACCATCTACACAATCCTAATGCGATGACTCAAAATGAACACCAGTTAACTCAAAACTGTTCAACACAAGTATGTCAATCTGAGAACCAGGAGTCAAATGAAGAAATGTATGAATTTAATTTTCTCGCTTTTTTGTTTTCCTCACACATTTGTCCTAAGCTAGATTGTTGATCATACTTATTGACTTACACACAGATGGTGAAACTGCTGAACGTTTTGAAGCATGCATCTCAATGCCGCCGCCGGGCAAGTAATGCTGAGCCATGCTCTCATCCAAACTGTTCTCAAATCAAGAAGTTGTTCTATCATGCCAGTAAGTGCAAAATTCAATTAAATGAGGGCTGCCAGCATTGTAAAAAGATATGGTTTATACTAACTTCTCACTCCAGGAATTGCAAAGACTCGGAATGTAGAATACCACGCTGCAGGTGTGTTACAATTAGAATTCTACACTACCTAGAGAATTTCGATATTCTATTTTATGAATTATTCTTTTACTAAACTATGCATAGATATACTTATTCATTTCAAAACAGAGTCATTTTTTTTTGGCTAATATATGCATACTTATTCTTTAATAGTTATGTTGATTTTGCAGTGATATGAAGAACCATGTAGAACAGAAATCAGTGCATTCTGAATCTTGCCGTAGAGCTGAAGTTTTGGAAAGCGATGGAGCAATCGTTGGCCAATGA
Protein sequence

>MS.gene010330.t1

MAATTARVVTTVMDLRVGMRTTVSDDDGGGGIEDINKQIEEDSLLAFETLLTGVQTMEDINKQIEDDPLLAFETLLTGVQSYSIRTLLQELKTLMDSSSDLDHLDTSQQVLRKHNQLLDSKADLMNKLRSAKSTQTQIDSESSTANAQIHKLSLQIDDLVKQRDDLKSAVNKCDVQKEKLMANALNGHNKVKSYFLIVLPWYFLRSVLFDNVVIEEVLHYLNKLLHIRRYESLMLIQSMKKRNQLQSGLVVRYKLVKDIQGRVVFSQEGTVAQKISEEGKDLEIHGMTDNNSMEIDIGANDNMHALVVVAESISIREVLSVDNQLAVKKQFLDIIPDENYPAEFFYRLRVSLKFKVLHQWDECNRWQHQICALYNKKADLDCSAEYICHVYHQKEIENGMHVPLPKAANSDAKDFPRTVLSDHLEIRLFERLMQERENWEKVEGNENLDEVLAAESLSIRKVLSINKQLKVNKQFLDIIPEENYPAEFSYRSRVILLFKLIEGADVCIFGMYLLMLKKATEENIVVGLTNIHDHFFLPTEKGNSKVTAFRLPYFEGDCWCGNAMEVAKTFEKESVGDYEKLLKQVSNRTLKDMGHAKPSKETLEMQKVGQNILPTRENFLIAHLQYSCLHCREVIVSGKRWFYTKCKKFQECERCHSYDQHTSKNGEVHTLCQAVVDDIPSNTKHNDIVLESRLFGNRDNFLIFCQKSQFQFDTLRRAKYSSMMILYHLHNPNAMTQNEHQLTQNCSTQMVKLLNVLKHASQCRRRASNAEPCSHPNCSQIKKLFYHASKCKIQLNEGCQHCKKIWFILTSHSRNCKDSECRIPRCSDMKNHVEQKSVHSESCRRAEVLESDGAIVGQ