AlfalfaGEDB | MS.gene010346

M. sativa cultivar XinJiangDaYe / MS.gene010346

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010346.t1	XP_003611730.1	82.1	251	41	1	1	251	1	247	9.80E-102	379.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010346.t1	Q9FHA7	56.8	192	69	4	17	204	4	185	2.2e-41	170.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010346.t1	G7K6N1	82.1	251	41	1	1	251	1	247	7.1e-102	379.8

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene010346.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010346.t1	MTR_5g017210	91.700	253	13	4	1	251	1	247	4.02e-138	388
MS.gene010346.t1	MTR_3g101810	54.067	209	79	7	25	216	40	248	1.70e-57	184
MS.gene010346.t1	MTR_8g093500	74.725	91	23	0	114	204	87	177	4.63e-46	155
MS.gene010346.t1	MTR_2g100490	57.252	131	48	3	84	207	227	356	3.85e-32	121
MS.gene010346.t1	MTR_8g015450	63.636	99	32	1	113	207	296	394	4.54e-29	114
MS.gene010346.t1	MTR_7g069590	68.966	58	18	0	147	204	32	89	4.35e-23	92.0
MS.gene010346.t1	MTR_5g079500	55.696	79	33	1	127	203	202	280	2.07e-20	88.6
MS.gene010346.t1	MTR_1g106460	53.731	67	31	0	137	203	234	300	1.29e-17	80.9
MS.gene010346.t1	MTR_1g018090	62.963	54	20	0	150	203	283	336	3.91e-17	80.1
MS.gene010346.t1	MTR_5g005110	62.963	54	20	0	150	203	233	286	6.20e-17	79.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010346.t1	AT5G67060	56.995	193	69	4	17	205	4	186	2.30e-51	167
MS.gene010346.t1	AT3G50330	53.881	219	70	6	14	224	4	199	3.02e-50	164
MS.gene010346.t1	AT5G67060	56.522	184	67	3	25	205	1	174	2.50e-48	159
MS.gene010346.t1	AT4G00120	66.667	96	29	1	116	208	87	182	3.85e-39	135
MS.gene010346.t1	AT3G21330	54.472	123	48	1	92	206	212	334	3.76e-38	137
MS.gene010346.t1	AT5G09750	75.556	90	22	0	114	203	92	181	7.43e-35	124
MS.gene010346.t1	AT5G01305	69.492	59	18	0	142	200	38	96	7.18e-22	89.4
MS.gene010346.t1	AT5G43175	50.725	69	34	0	135	203	126	194	7.66e-19	82.8
MS.gene010346.t1	AT4G33880	59.677	62	25	0	142	203	267	328	1.58e-18	84.0
MS.gene010346.t1	AT5G37800	56.164	73	25	1	138	203	191	263	3.99e-18	82.4

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Find 58 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAATGTGAAGATTTCAA+AGG	0.590303	5.4:+10338123	MS.gene010346:CDS
AAAGAATTAGTGAAAGAATA+AGG	0.285456	5.4:+10338179	MS.gene010346:CDS
AACAGAGTTCAAATCCCATC+AGG	0.513806	5.4:+10337968	MS.gene010346:CDS
AATAGACCTTTGAATGTTGT+TGG	0.403761	5.4:+10338322	MS.gene010346:CDS
AATGGTGGATTGATCAATGA+AGG	0.607991	5.4:-10337893	None:intergenic
AATTATTCTTCTTTGCTTAG+AGG	0.274535	5.4:+10338376	MS.gene010346:CDS
AATTGTTGGTTCTTCCTGAT+GGG	0.499420	5.4:-10337982	None:intergenic
ACAAACAATGATTTCCCTTA+TGG	0.235794	5.4:+10337842	MS.gene010346:CDS
ACTAAGCATTTGTTTGGAAG+TGG	0.402015	5.4:-10338428	None:intergenic
ACTCTGTTGGGTTGAAGCGA+CGG	0.575367	5.4:-10337953	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAGACCTTTGAATGTTGT+TGG	+	chr5.4:10338322-10338341	MS.gene010346:CDS	30.0%
ACAAACAATGATTTCCCTTA+TGG	+	chr5.4:10337842-10337861	MS.gene010346:CDS	30.0%
ATAGACCTTTGAATGTTGTT+GGG	+	chr5.4:10338323-10338342	MS.gene010346:CDS	30.0%
ATAGGAGAATGAAGAATTGT+TGG	-	chr5.4:10337999-10338018	None:intergenic	30.0%
ATCTTCACATTCTTTCTCTT+TGG	-	chr5.4:10338118-10338137	None:intergenic	30.0%
GAAGACATACTGAAAACTTT+GGG	+	chr5.4:10337704-10337723	MS.gene010346:CDS	30.0%
TGAAGACATACTGAAAACTT+TGG	+	chr5.4:10337703-10337722	MS.gene010346:CDS	30.0%
TGAAGAGGATAAGACAATAA+TGG	-	chr5.4:10337914-10337933	None:intergenic	30.0%
ACTAAGCATTTGTTTGGAAG+TGG	-	chr5.4:10338431-10338450	None:intergenic	35.0%
AGAGATGATATTTCGAATGG+CGG	+	chr5.4:10338051-10338070	MS.gene010346:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	10337698	10338453	10337698	ID=MS.gene010346
chr5.4	mRNA	10337698	10338453	10337698	ID=MS.gene010346.t1;Parent=MS.gene010346
chr5.4	exon	10337698	10338453	10337698	ID=MS.gene010346.t1.exon1;Parent=MS.gene010346.t1
chr5.4	CDS	10337698	10338453	10337698	ID=cds.MS.gene010346.t1;Parent=MS.gene010346.t1

Gene Sequence

>MS.gene010346

ATGGATGAAGACATACTGAAAACTTTGGGAAATGTTGAAAGCAACATGGACATGATGACAATGATGATGCAAATGGAAAAGTTCCCTGATTCATATGAACCTTTTTATAGCAACACCAATAACAACACTCTCAATCTCAATCTCACAAACAATGATTTCCCTTATGGAAACTCAAATGTAACTTCATCACTCACACCTTCATTGATCAATCCACCATTATTGTCTTATCCTCTTCAACAACCAATGACACCACCGCCGTCGCTTCAACCCAACAGAGTTCAAATCCCATCAGGAAGAACCAACAATTCTTCATTCTCCTATCCCGAGAAGAAGAATTCAATGGCAGCAATGAGAGAGATGATATTTCGAATGGCGGTTATGCAACCGATTAACATAGACCCAGAAACCATTAAACCACCAAAGAGAAAGAATGTGAAGATTTCAAAGGATCCACAAAGTGTAGCAGCAAGACATAGAAGAGAAAGAATTAGTGAAAGAATAAGGATATTACAAAGATTAGTACCTGGTGGAACAAAAATGGACACTGCTTCTATGTTAGATGAAGCTATACATTATGTGAAGTTTTTGAAGAAACAAGTTCAAACGTTGGAACAAGTTGGGGCAAATAGACCTTTGAATGTTGTTGGGTTTCAAGGAGTGGTTTCTAATGCTAATTTTAATTATTCTTCTTTGCTTAGAGGTTGTTCATCACCTTGCCAAATGGTGGGTCCCACTTCCAAACAAATGCTTAGTTGA

Protein sequence

>MS.gene010346.t1

MDEDILKTLGNVESNMDMMTMMMQMEKFPDSYEPFYSNTNNNTLNLNLTNNDFPYGNSNVTSSLTPSLINPPLLSYPLQQPMTPPPSLQPNRVQIPSGRTNNSSFSYPEKKNSMAAMREMIFRMAVMQPINIDPETIKPPKRKNVKISKDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIHYVKFLKKQVQTLEQVGANRPLNVVGFQGVVSNANFNYSSLLRGCSSPCQMVGPTSKQMLS