AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010346


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010346.t1 MTR_5g017210 91.700 253 13 4 1 251 1 247 4.02e-138 388
MS.gene010346.t1 MTR_3g101810 54.067 209 79 7 25 216 40 248 1.70e-57 184
MS.gene010346.t1 MTR_8g093500 74.725 91 23 0 114 204 87 177 4.63e-46 155
MS.gene010346.t1 MTR_2g100490 57.252 131 48 3 84 207 227 356 3.85e-32 121
MS.gene010346.t1 MTR_8g015450 63.636 99 32 1 113 207 296 394 4.54e-29 114
MS.gene010346.t1 MTR_7g069590 68.966 58 18 0 147 204 32 89 4.35e-23 92.0
MS.gene010346.t1 MTR_5g079500 55.696 79 33 1 127 203 202 280 2.07e-20 88.6
MS.gene010346.t1 MTR_1g106460 53.731 67 31 0 137 203 234 300 1.29e-17 80.9
MS.gene010346.t1 MTR_1g018090 62.963 54 20 0 150 203 283 336 3.91e-17 80.1
MS.gene010346.t1 MTR_5g005110 62.963 54 20 0 150 203 233 286 6.20e-17 79.3
MS.gene010346.t1 MTR_1g106470 51.948 77 32 2 132 203 182 258 8.58e-17 78.2
MS.gene010346.t1 MTR_3g103030 55.556 63 28 0 141 203 229 291 3.61e-16 77.0
MS.gene010346.t1 MTR_4g131160 43.564 101 47 3 122 212 172 272 8.56e-15 73.6
MS.gene010346.t1 MTR_4g131160 43.564 101 47 3 122 212 175 275 8.98e-15 73.6
MS.gene010346.t1 MTR_1g080890 51.429 70 34 0 134 203 186 255 1.14e-14 72.8
MS.gene010346.t1 MTR_1g106480 64.444 45 16 0 159 203 87 131 1.18e-13 67.0
MS.gene010346.t1 MTR_2g067000 46.667 75 33 1 137 204 146 220 1.13e-12 66.6
MS.gene010346.t1 MTR_1g060700 57.407 54 23 0 151 204 357 410 4.71e-12 65.5
MS.gene010346.t1 MTR_4g033295 50.769 65 31 1 142 205 119 183 5.64e-12 63.2
MS.gene010346.t1 MTR_4g033295 50.000 66 32 1 142 206 119 184 1.03e-11 62.4
MS.gene010346.t1 MTR_2g089070 48.148 54 28 0 151 204 146 199 8.98e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010346.t1 AT5G67060 56.995 193 69 4 17 205 4 186 2.30e-51 167
MS.gene010346.t1 AT3G50330 53.881 219 70 6 14 224 4 199 3.02e-50 164
MS.gene010346.t1 AT5G67060 56.522 184 67 3 25 205 1 174 2.50e-48 159
MS.gene010346.t1 AT4G00120 66.667 96 29 1 116 208 87 182 3.85e-39 135
MS.gene010346.t1 AT3G21330 54.472 123 48 1 92 206 212 334 3.76e-38 137
MS.gene010346.t1 AT5G09750 75.556 90 22 0 114 203 92 181 7.43e-35 124
MS.gene010346.t1 AT5G01305 69.492 59 18 0 142 200 38 96 7.18e-22 89.4
MS.gene010346.t1 AT5G43175 50.725 69 34 0 135 203 126 194 7.66e-19 82.8
MS.gene010346.t1 AT4G33880 59.677 62 25 0 142 203 267 328 1.58e-18 84.0
MS.gene010346.t1 AT5G37800 56.164 73 25 1 138 203 191 263 3.99e-18 82.4
MS.gene010346.t1 AT1G66470 67.273 55 18 0 149 203 203 257 5.31e-18 81.6
MS.gene010346.t1 AT1G27740 51.471 68 33 0 136 203 162 229 5.33e-18 81.3
MS.gene010346.t1 AT2G14760 58.065 62 26 0 142 203 238 299 1.67e-17 80.9
MS.gene010346.t1 AT4G30980 39.200 125 75 1 79 203 69 192 7.69e-17 78.6
MS.gene010346.t1 AT2G24260 52.703 74 29 1 136 203 24 97 1.30e-16 77.0
MS.gene010346.t1 AT4G30980 39.200 125 75 1 79 203 117 240 1.56e-16 78.2
MS.gene010346.t1 AT2G24260 52.703 74 29 1 136 203 127 200 3.36e-16 77.4
MS.gene010346.t1 AT5G58010 54.545 66 30 0 138 203 96 161 8.93e-15 72.8
MS.gene010346.t1 AT2G14760 48.000 75 26 1 142 203 238 312 3.22e-14 71.6
MS.gene010346.t1 AT4G09180 43.210 81 45 1 124 203 167 247 7.32e-11 61.2

Find 58 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGTGGTTTAATGGTTTC+TGG 0.032783 5.4:-10338097 None:intergenic
CTTTCTCTTTGGTGGTTTAA+TGG 0.129321 5.4:-10338104 None:intergenic
ACAAACAATGATTTCCCTTA+TGG 0.235794 5.4:+10337842 MS.gene010346:CDS
ATCTTCACATTCTTTCTCTT+TGG 0.246196 5.4:-10338115 None:intergenic
AATTATTCTTCTTTGCTTAG+AGG 0.274535 5.4:+10338376 MS.gene010346:CDS
GAGATTGAGAGTGTTGTTAT+TGG 0.283226 5.4:-10337813 None:intergenic
AAAGAATTAGTGAAAGAATA+AGG 0.285456 5.4:+10338179 MS.gene010346:CDS
TTATTGGTGTTGCTATAAAA+AGG 0.298991 5.4:-10337797 None:intergenic
TTGGTGGTTTAATGGTTTCT+GGG 0.314323 5.4:-10338096 None:intergenic
TGCCATTGAATTCTTCTTCT+CGG 0.319338 5.4:-10338020 None:intergenic
TGTCTTGCTGCTACACTTTG+TGG 0.337683 5.4:-10338148 None:intergenic
TTCACATTCTTTCTCTTTGG+TGG 0.352271 5.4:-10338112 None:intergenic
TTCAAACGTTGGAACAAGTT+GGG 0.370508 5.4:+10338296 MS.gene010346:CDS
TTGAATGTTGTTGGGTTTCA+AGG 0.375435 5.4:+10338331 MS.gene010346:CDS
TGAAGACATACTGAAAACTT+TGG 0.382012 5.4:+10337703 MS.gene010346:CDS
GTTTCTGGGTCTATGTTAAT+CGG 0.384832 5.4:-10338082 None:intergenic
TGATGGGATTTGAACTCTGT+TGG 0.387374 5.4:-10337966 None:intergenic
GCCATTGAATTCTTCTTCTC+GGG 0.399282 5.4:-10338019 None:intergenic
ACTAAGCATTTGTTTGGAAG+TGG 0.402015 5.4:-10338428 None:intergenic
AATAGACCTTTGAATGTTGT+TGG 0.403761 5.4:+10338322 MS.gene010346:CDS
GTTACATTTGAGTTTCCATA+AGG 0.409196 5.4:-10337857 None:intergenic
TGGAAGTGGGACCCACCATT+TGG 0.414540 5.4:-10338414 None:intergenic
GATGGGATTTGAACTCTGTT+GGG 0.418955 5.4:-10337965 None:intergenic
GTGTCAACTAAGCATTTGTT+TGG 0.421668 5.4:-10338434 None:intergenic
GAAGACATACTGAAAACTTT+GGG 0.432163 5.4:+10337704 MS.gene010346:CDS
TTCATCACCTTGCCAAATGG+TGG 0.447650 5.4:+10338402 MS.gene010346:CDS
TGAAGAGGATAAGACAATAA+TGG 0.449931 5.4:-10337911 None:intergenic
ATAGACCTTTGAATGTTGTT+GGG 0.458742 5.4:+10338323 MS.gene010346:CDS
TGAATTCTTCTTCTCGGGAT+AGG 0.459318 5.4:-10338014 None:intergenic
AGCGACGGCGGTGGTGTCAT+TGG 0.462107 5.4:-10337938 None:intergenic
ATAGGAGAATGAAGAATTGT+TGG 0.474836 5.4:-10337996 None:intergenic
TTACATTTGAGTTTCCATAA+GGG 0.484501 5.4:-10337856 None:intergenic
GTTCAAACGTTGGAACAAGT+TGG 0.486495 5.4:+10338295 MS.gene010346:CDS
GATGACAATGATGATGCAAA+TGG 0.498844 5.4:+10337751 MS.gene010346:CDS
AATTGTTGGTTCTTCCTGAT+GGG 0.499420 5.4:-10337982 None:intergenic
GAATTGTTGGTTCTTCCTGA+TGG 0.503297 5.4:-10337983 None:intergenic
TTGTTCATCACCTTGCCAAA+TGG 0.505893 5.4:+10338399 MS.gene010346:CDS
AACAGAGTTCAAATCCCATC+AGG 0.513806 5.4:+10337968 MS.gene010346:CDS
TGTTGTTGGGTTTCAAGGAG+TGG 0.515205 5.4:+10338336 MS.gene010346:CDS
CTAAGCATTTGTTTGGAAGT+GGG 0.548242 5.4:-10338427 None:intergenic
TTGGGTTGAAGCGACGGCGG+TGG 0.553364 5.4:-10337947 None:intergenic
GAAGAAACAAGTTCAAACGT+TGG 0.554879 5.4:+10338285 MS.gene010346:CDS
TCATCACCTTGCCAAATGGT+GGG 0.555921 5.4:+10338403 MS.gene010346:CDS
TGAAACCCAACAACATTCAA+AGG 0.560330 5.4:-10338328 None:intergenic
TCAAACGTTGGAACAAGTTG+GGG 0.569007 5.4:+10338297 MS.gene010346:CDS
ACTCTGTTGGGTTGAAGCGA+CGG 0.575367 5.4:-10337953 None:intergenic
GAGAGAGATGATATTTCGAA+TGG 0.580908 5.4:+10338048 MS.gene010346:CDS
GGTGTCATTGGTTGTTGAAG+AGG 0.589982 5.4:-10337926 None:intergenic
AAAGAATGTGAAGATTTCAA+AGG 0.590303 5.4:+10338123 MS.gene010346:CDS
TCCCGAGAAGAAGAATTCAA+TGG 0.593246 5.4:+10338018 MS.gene010346:CDS
AATGGTGGATTGATCAATGA+AGG 0.607991 5.4:-10337893 None:intergenic
AGAGGATAAGACAATAATGG+TGG 0.609384 5.4:-10337908 None:intergenic
AGAGATGATATTTCGAATGG+CGG 0.620797 5.4:+10338051 MS.gene010346:CDS
GTGGGACCCACCATTTGGCA+AGG 0.634945 5.4:-10338409 None:intergenic
GGGAAATGTTGAAAGCAACA+TGG 0.641963 5.4:+10337724 MS.gene010346:CDS
CTGTTGGGTTGAAGCGACGG+CGG 0.671897 5.4:-10337950 None:intergenic
ATATTACAAAGATTAGTACC+TGG 0.683168 5.4:+10338202 MS.gene010346:CDS
TTACAAAGATTAGTACCTGG+TGG 0.689802 5.4:+10338205 MS.gene010346:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAATTAGTGAAAGAATA+AGG + chr5.4:10338179-10338198 MS.gene010346:CDS 20.0%
! AAAAAGGTTCATATGAATCA+GGG - chr5.4:10337784-10337803 None:intergenic 25.0%
! AAAGAATGTGAAGATTTCAA+AGG + chr5.4:10338123-10338142 MS.gene010346:CDS 25.0%
! AATTATTCTTCTTTGCTTAG+AGG + chr5.4:10338376-10338395 MS.gene010346:CDS 25.0%
! ATATTACAAAGATTAGTACC+TGG + chr5.4:10338202-10338221 MS.gene010346:CDS 25.0%
! TAAAAAGGTTCATATGAATC+AGG - chr5.4:10337785-10337804 None:intergenic 25.0%
!! TTACATTTGAGTTTCCATAA+GGG - chr5.4:10337859-10337878 None:intergenic 25.0%
!!! TTATTGGTGTTGCTATAAAA+AGG - chr5.4:10337800-10337819 None:intergenic 25.0%
AATAGACCTTTGAATGTTGT+TGG + chr5.4:10338322-10338341 MS.gene010346:CDS 30.0%
ACAAACAATGATTTCCCTTA+TGG + chr5.4:10337842-10337861 MS.gene010346:CDS 30.0%
ATAGACCTTTGAATGTTGTT+GGG + chr5.4:10338323-10338342 MS.gene010346:CDS 30.0%
ATAGGAGAATGAAGAATTGT+TGG - chr5.4:10337999-10338018 None:intergenic 30.0%
ATCTTCACATTCTTTCTCTT+TGG - chr5.4:10338118-10338137 None:intergenic 30.0%
GAAGACATACTGAAAACTTT+GGG + chr5.4:10337704-10337723 MS.gene010346:CDS 30.0%
TGAAGACATACTGAAAACTT+TGG + chr5.4:10337703-10337722 MS.gene010346:CDS 30.0%
TGAAGAGGATAAGACAATAA+TGG - chr5.4:10337914-10337933 None:intergenic 30.0%
! GTTACATTTGAGTTTCCATA+AGG - chr5.4:10337860-10337879 None:intergenic 30.0%
ACTAAGCATTTGTTTGGAAG+TGG - chr5.4:10338431-10338450 None:intergenic 35.0%
AGAGATGATATTTCGAATGG+CGG + chr5.4:10338051-10338070 MS.gene010346:CDS 35.0%
AGAGGATAAGACAATAATGG+TGG - chr5.4:10337911-10337930 None:intergenic 35.0%
CTAAGCATTTGTTTGGAAGT+GGG - chr5.4:10338430-10338449 None:intergenic 35.0%
GAAGAAACAAGTTCAAACGT+TGG + chr5.4:10338285-10338304 MS.gene010346:CDS 35.0%
GAGAGAGATGATATTTCGAA+TGG + chr5.4:10338048-10338067 MS.gene010346:CDS 35.0%
TGAAACCCAACAACATTCAA+AGG - chr5.4:10338331-10338350 None:intergenic 35.0%
TGCCATTGAATTCTTCTTCT+CGG - chr5.4:10338023-10338042 None:intergenic 35.0%
TTACAAAGATTAGTACCTGG+TGG + chr5.4:10338205-10338224 MS.gene010346:CDS 35.0%
TTCAAACGTTGGAACAAGTT+GGG + chr5.4:10338296-10338315 MS.gene010346:CDS 35.0%
TTCACATTCTTTCTCTTTGG+TGG - chr5.4:10338115-10338134 None:intergenic 35.0%
TTGAATGTTGTTGGGTTTCA+AGG + chr5.4:10338331-10338350 MS.gene010346:CDS 35.0%
! CTTTCTCTTTGGTGGTTTAA+TGG - chr5.4:10338107-10338126 None:intergenic 35.0%
! GATGACAATGATGATGCAAA+TGG + chr5.4:10337751-10337770 MS.gene010346:CDS 35.0%
! GTTTCTGGGTCTATGTTAAT+CGG - chr5.4:10338085-10338104 None:intergenic 35.0%
! TTGGTGGTTTAATGGTTTCT+GGG - chr5.4:10338099-10338118 None:intergenic 35.0%
! TTTGGTGGTTTAATGGTTTC+TGG - chr5.4:10338100-10338119 None:intergenic 35.0%
!! AATGGTGGATTGATCAATGA+AGG - chr5.4:10337896-10337915 None:intergenic 35.0%
!! AATTGTTGGTTCTTCCTGAT+GGG - chr5.4:10337985-10338004 None:intergenic 35.0%
!! GAGATTGAGAGTGTTGTTAT+TGG - chr5.4:10337816-10337835 None:intergenic 35.0%
AACAGAGTTCAAATCCCATC+AGG + chr5.4:10337968-10337987 MS.gene010346:CDS 40.0%
AGTACCTGGTGGAACAAAAA+TGG + chr5.4:10338216-10338235 MS.gene010346:CDS 40.0%
GATGGGATTTGAACTCTGTT+GGG - chr5.4:10337968-10337987 None:intergenic 40.0%
GCCATTGAATTCTTCTTCTC+GGG - chr5.4:10338022-10338041 None:intergenic 40.0%
GGGAAATGTTGAAAGCAACA+TGG + chr5.4:10337724-10337743 MS.gene010346:CDS 40.0%
GTTCAAACGTTGGAACAAGT+TGG + chr5.4:10338295-10338314 MS.gene010346:CDS 40.0%
TCAAACGTTGGAACAAGTTG+GGG + chr5.4:10338297-10338316 MS.gene010346:CDS 40.0%
TCCCGAGAAGAAGAATTCAA+TGG + chr5.4:10338018-10338037 MS.gene010346:CDS 40.0%
TGAATTCTTCTTCTCGGGAT+AGG - chr5.4:10338017-10338036 None:intergenic 40.0%
TGATGGGATTTGAACTCTGT+TGG - chr5.4:10337969-10337988 None:intergenic 40.0%
TTGTTCATCACCTTGCCAAA+TGG + chr5.4:10338399-10338418 MS.gene010346:CDS 40.0%
!! GAATTGTTGGTTCTTCCTGA+TGG - chr5.4:10337986-10338005 None:intergenic 40.0%
TCATCACCTTGCCAAATGGT+GGG + chr5.4:10338403-10338422 MS.gene010346:CDS 45.0%
TGTCTTGCTGCTACACTTTG+TGG - chr5.4:10338151-10338170 None:intergenic 45.0%
TGTTGTTGGGTTTCAAGGAG+TGG + chr5.4:10338336-10338355 MS.gene010346:CDS 45.0%
TTCATCACCTTGCCAAATGG+TGG + chr5.4:10338402-10338421 MS.gene010346:CDS 45.0%
!! GGTGTCATTGGTTGTTGAAG+AGG - chr5.4:10337929-10337948 None:intergenic 45.0%
!! GTGTCCATTTTTGTTCCACC+AGG - chr5.4:10338223-10338242 None:intergenic 45.0%
!! ACTCTGTTGGGTTGAAGCGA+CGG - chr5.4:10337956-10337975 None:intergenic 50.0%
TGGAAGTGGGACCCACCATT+TGG - chr5.4:10338417-10338436 None:intergenic 55.0%
GTGGGACCCACCATTTGGCA+AGG - chr5.4:10338412-10338431 None:intergenic 60.0%
!! CTGTTGGGTTGAAGCGACGG+CGG - chr5.4:10337953-10337972 None:intergenic 60.0%
!! AGCGACGGCGGTGGTGTCAT+TGG - chr5.4:10337941-10337960 None:intergenic 65.0%
!! TTGGGTTGAAGCGACGGCGG+TGG - chr5.4:10337950-10337969 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 10337698 10338453 10337698 ID=MS.gene010346
chr5.4 mRNA 10337698 10338453 10337698 ID=MS.gene010346.t1;Parent=MS.gene010346
chr5.4 exon 10337698 10338453 10337698 ID=MS.gene010346.t1.exon1;Parent=MS.gene010346.t1
chr5.4 CDS 10337698 10338453 10337698 ID=cds.MS.gene010346.t1;Parent=MS.gene010346.t1
Gene Sequence

>MS.gene010346

ATGGATGAAGACATACTGAAAACTTTGGGAAATGTTGAAAGCAACATGGACATGATGACAATGATGATGCAAATGGAAAAGTTCCCTGATTCATATGAACCTTTTTATAGCAACACCAATAACAACACTCTCAATCTCAATCTCACAAACAATGATTTCCCTTATGGAAACTCAAATGTAACTTCATCACTCACACCTTCATTGATCAATCCACCATTATTGTCTTATCCTCTTCAACAACCAATGACACCACCGCCGTCGCTTCAACCCAACAGAGTTCAAATCCCATCAGGAAGAACCAACAATTCTTCATTCTCCTATCCCGAGAAGAAGAATTCAATGGCAGCAATGAGAGAGATGATATTTCGAATGGCGGTTATGCAACCGATTAACATAGACCCAGAAACCATTAAACCACCAAAGAGAAAGAATGTGAAGATTTCAAAGGATCCACAAAGTGTAGCAGCAAGACATAGAAGAGAAAGAATTAGTGAAAGAATAAGGATATTACAAAGATTAGTACCTGGTGGAACAAAAATGGACACTGCTTCTATGTTAGATGAAGCTATACATTATGTGAAGTTTTTGAAGAAACAAGTTCAAACGTTGGAACAAGTTGGGGCAAATAGACCTTTGAATGTTGTTGGGTTTCAAGGAGTGGTTTCTAATGCTAATTTTAATTATTCTTCTTTGCTTAGAGGTTGTTCATCACCTTGCCAAATGGTGGGTCCCACTTCCAAACAAATGCTTAGTTGA

Protein sequence

>MS.gene010346.t1

MDEDILKTLGNVESNMDMMTMMMQMEKFPDSYEPFYSNTNNNTLNLNLTNNDFPYGNSNVTSSLTPSLINPPLLSYPLQQPMTPPPSLQPNRVQIPSGRTNNSSFSYPEKKNSMAAMREMIFRMAVMQPINIDPETIKPPKRKNVKISKDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIHYVKFLKKQVQTLEQVGANRPLNVVGFQGVVSNANFNYSSLLRGCSSPCQMVGPTSKQMLS