AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010368

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010368.t1 MTR_5g017500 84.400 250 18 3 1 250 1 229 2.04e-145 406
MS.gene010368.t1 MTR_4g117990 46.789 109 56 1 14 122 36 142 2.22e-27 107
MS.gene010368.t1 MTR_7g081190 50.000 102 48 2 19 118 43 143 4.25e-22 94.4
MS.gene010368.t1 MTR_1g061600 30.370 135 75 4 22 143 133 261 5.33e-16 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010368.t1 AT3G24860 34.201 269 134 6 11 249 54 309 8.57e-44 150
MS.gene010368.t1 AT2G44730 43.478 115 55 2 9 119 51 159 3.47e-22 94.4
MS.gene010368.t1 AT3G54390 27.966 236 131 8 22 233 38 258 2.39e-14 71.6
MS.gene010368.t1 AT3G10030 34.653 101 60 2 22 122 161 255 2.51e-14 72.4
MS.gene010368.t1 AT3G10030 34.653 101 60 2 22 122 161 255 3.11e-14 72.0
MS.gene010368.t1 AT3G54390 27.848 237 132 8 22 234 54 275 3.16e-14 71.2
MS.gene010368.t1 AT3G58630 29.915 117 69 2 22 128 26 139 3.50e-13 68.2
MS.gene010368.t1 AT3G58630 29.915 117 69 2 22 128 26 139 3.92e-13 68.2

Find 80 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTGTTACTGTTGGTTTC+TGG 0.153076 5.4:-10607019 None:intergenic
GTAGGATCTGATGAGGTTAA+TGG 0.242282 5.4:-10606616 None:intergenic
GATTCTATTGGAAAGGCATT+TGG 0.282980 5.4:+10607257 MS.gene010368:CDS
TGGAGGATTGTTGATTCTAT+TGG 0.285128 5.4:+10607245 MS.gene010368:CDS
ACGACATTGAAGAGCGGTTT+TGG 0.311108 5.4:-10606745 None:intergenic
TGATGAGGTTAATGGTTTCT+TGG 0.328328 5.4:-10606608 None:intergenic
TCTTGGTGGGTCCATGGAAT+GGG 0.347722 5.4:-10606591 None:intergenic
TTCTTGGTGGGTCCATGGAA+TGG 0.347924 5.4:-10606592 None:intergenic
CCGTCGCTATCGTCGGAGAT+AGG 0.363885 5.4:-10606930 None:intergenic
GATTAGAGATGGAGAATAAG+AGG 0.371813 5.4:+10607198 MS.gene010368:CDS
ATTCTATTGGAAAGGCATTT+GGG 0.373738 5.4:+10607258 MS.gene010368:CDS
TTTCAGATCGGTGGCGTTGA+CGG 0.386482 5.4:-10606785 None:intergenic
ATGGGTTGAGGTTTCTTTGT+GGG 0.390802 5.4:-10606573 None:intergenic
GAAATGGTATTCACTCAAAC+GGG 0.393548 5.4:+10606644 MS.gene010368:CDS
GCCCACTCCGCGGCAGTCAA+TGG 0.394607 5.4:+10606667 MS.gene010368:CDS
CCGCCGTTTCTTCTCGATCC+TGG 0.399673 5.4:+10606832 MS.gene010368:CDS
GAAAGAGGTCGGACGGAGAT+AGG 0.403361 5.4:-10606897 None:intergenic
CCCATTGACTGCCGCGGAGT+GGG 0.424106 5.4:-10606668 None:intergenic
TGCAGAGAGGATTGTAGGGT+TGG 0.427539 5.4:+10607133 MS.gene010368:CDS
ATGGTTTCTTGGTGGGTCCA+TGG 0.435098 5.4:-10606597 None:intergenic
ATAGTGCTGCTAGGTCTGTT+AGG 0.441997 5.4:+10606970 MS.gene010368:CDS
GGAGGAGGAAGACGAAGAGG+AGG 0.442580 5.4:+10607070 MS.gene010368:CDS
TGGGTCCATGGAATGGGTTG+AGG 0.452120 5.4:-10606585 None:intergenic
CCCACTCCGCGGCAGTCAAT+GGG 0.457508 5.4:+10606668 MS.gene010368:CDS
AACTGATGATGAAGAGGAGG+AGG 0.459365 5.4:+10607055 MS.gene010368:CDS
CGTTGTTGTCTTGTTACTGT+TGG 0.464913 5.4:-10607026 None:intergenic
TCAGATCCTACCAAGAGAAA+TGG 0.466613 5.4:+10606628 MS.gene010368:CDS
AGAGAGGTTTAGATTAGAGA+TGG 0.466781 5.4:+10607187 MS.gene010368:CDS
TTGAGGTTTCTTTGTGGGAA+GGG 0.468718 5.4:-10606568 None:intergenic
TCCCATTGACTGCCGCGGAG+TGG 0.479922 5.4:-10606669 None:intergenic
AGAAATGGTATTCACTCAAA+CGG 0.486979 5.4:+10606643 MS.gene010368:CDS
GTATGATACTTGAATCTCAA+TGG 0.493726 5.4:+10607225 MS.gene010368:CDS
GAGGAGGAAGACGAAGAGGA+GGG 0.496326 5.4:+10607071 MS.gene010368:CDS
GAGGTTAATGGTTTCTTGGT+GGG 0.497980 5.4:-10606604 None:intergenic
CGTCGCTATCGTCGGAGATA+GGG 0.505081 5.4:-10606929 None:intergenic
GGAAGGGAAAGAGTGAGTTC+TGG 0.514086 5.4:-10606552 None:intergenic
TGAGTGAATACCATTTCTCT+TGG 0.519977 5.4:-10606638 None:intergenic
TGAATACCATTTCTCTTGGT+AGG 0.521476 5.4:-10606634 None:intergenic
GATTGTTGATTCTATTGGAA+AGG 0.528242 5.4:+10607250 MS.gene010368:CDS
AGACGAAGAGGAGGGAGTGA+TGG 0.530323 5.4:+10607079 MS.gene010368:CDS
TGAGGTTAATGGTTTCTTGG+TGG 0.535347 5.4:-10606605 None:intergenic
CTGCCAGGATCGAGAAGAAA+CGG 0.535951 5.4:-10606835 None:intergenic
TCTTCAATGTCGTCATAAGA+TGG 0.539576 5.4:+10606755 MS.gene010368:CDS
AATGGGTTGAGGTTTCTTTG+TGG 0.544865 5.4:-10606574 None:intergenic
AGAAACCTCAACCCATTCCA+TGG 0.546082 5.4:+10606580 MS.gene010368:CDS
TAAGAAGATGGAGATGATGA+AGG 0.556347 5.4:+10607160 MS.gene010368:CDS
AGAGGAGGAGGAAGACGAAG+AGG 0.557060 5.4:+10607067 MS.gene010368:CDS
CGGCAGTCAATGGGAAGAAG+TGG 0.558439 5.4:+10606677 MS.gene010368:CDS
ATAGGGTGCTTCTGAGAAAG+AGG 0.564002 5.4:-10606912 None:intergenic
GTTGAGGTTTCTTTGTGGGA+AGG 0.565690 5.4:-10606569 None:intergenic
GGTCGGACGGAGATAGGTAA+AGG 0.569635 5.4:-10606891 None:intergenic
AGGGTTGGAAAATAAGAAGA+TGG 0.571203 5.4:+10607148 MS.gene010368:CDS
GGATCGAGAAGAAACGGCGG+AGG 0.574532 5.4:-10606829 None:intergenic
CTTCTTCCCATTGACTGCCG+CGG 0.577676 5.4:-10606674 None:intergenic
CTGCTAGGTCTGTTAGGTCA+AGG 0.578720 5.4:+10606976 MS.gene010368:CDS
CCGACGATAGCGACGGTGAG+AGG 0.582725 5.4:+10606937 MS.gene010368:CDS
CCTCTCACCGTCGCTATCGT+CGG 0.588877 5.4:-10606937 None:intergenic
TCTCTTGGTAGGATCTGATG+AGG 0.595101 5.4:-10606623 None:intergenic
CGACGATAGCGACGGTGAGA+GGG 0.604292 5.4:+10606938 MS.gene010368:CDS
AGTGAGTTCTGGTGAAGCGG+TGG 0.606604 5.4:-10606541 None:intergenic
CATGAGACGAAAATACTGCC+AGG 0.607104 5.4:-10606850 None:intergenic
CGGAGGAAGTAGCGGTGAGG+CGG 0.610003 5.4:-10606812 None:intergenic
AAGAGTGAGTTCTGGTGAAG+CGG 0.610541 5.4:-10606544 None:intergenic
CTTCTGAGAAAGAGGTCGGA+CGG 0.613702 5.4:-10606904 None:intergenic
GGTGCTTCTGAGAAAGAGGT+CGG 0.636676 5.4:-10606908 None:intergenic
AGATGATGAAGGAAACAGAG+AGG 0.638850 5.4:+10607171 MS.gene010368:CDS
AGAAACGGCGGAGGAAGTAG+CGG 0.642928 5.4:-10606820 None:intergenic
CTCAAACGGGGCCCACTCCG+CGG 0.643190 5.4:+10606657 MS.gene010368:CDS
TGATGAAACTGATGATGAAG+AGG 0.652723 5.4:+10607049 MS.gene010368:CDS
TGATACTTGAATCTCAATGG+AGG 0.662965 5.4:+10607228 MS.gene010368:CDS
CTCATGGATGATCTTGAACG+CGG 0.669974 5.4:+10606867 MS.gene010368:CDS
TGAAACTGATGATGAAGAGG+AGG 0.676552 5.4:+10607052 MS.gene010368:CDS
CTTATGACGACATTGAAGAG+CGG 0.678566 5.4:-10606751 None:intergenic
CCTATCTCCGACGATAGCGA+CGG 0.701116 5.4:+10606930 MS.gene010368:CDS
GTAATCGTAGCCGCAACGAG+CGG 0.705311 5.4:-10606712 None:intergenic
CGGCGGAGGAAGTAGCGGTG+AGG 0.714161 5.4:-10606815 None:intergenic
GATGGCGAAGAGTTTGACAG+CGG 0.738106 5.4:+10607097 MS.gene010368:CDS
CCAGGATCGAGAAGAAACGG+CGG 0.744864 5.4:-10606832 None:intergenic
AAATGGTATTCACTCAAACG+GGG 0.751020 5.4:+10606645 MS.gene010368:CDS
GTAGTCGTAGCCGCTCGTTG+CGG 0.770618 5.4:+10606702 MS.gene010368:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAATGGTATTCACTCAAA+CGG + chr5.4:10606643-10606662 MS.gene010368:CDS 30.0%
GTATGATACTTGAATCTCAA+TGG + chr5.4:10607225-10607244 MS.gene010368:CDS 30.0%
!! ATTCTATTGGAAAGGCATTT+GGG + chr5.4:10607258-10607277 MS.gene010368:CDS 30.0%
!! GATTGTTGATTCTATTGGAA+AGG + chr5.4:10607250-10607269 MS.gene010368:CDS 30.0%
AAATGGTATTCACTCAAACG+GGG + chr5.4:10606645-10606664 MS.gene010368:CDS 35.0%
AGAGAGGTTTAGATTAGAGA+TGG + chr5.4:10607187-10607206 MS.gene010368:CDS 35.0%
AGGGTTGGAAAATAAGAAGA+TGG + chr5.4:10607148-10607167 MS.gene010368:CDS 35.0%
GAAATGGTATTCACTCAAAC+GGG + chr5.4:10606644-10606663 MS.gene010368:CDS 35.0%
GATTAGAGATGGAGAATAAG+AGG + chr5.4:10607198-10607217 MS.gene010368:CDS 35.0%
TAAGAAGATGGAGATGATGA+AGG + chr5.4:10607160-10607179 MS.gene010368:CDS 35.0%
TCTTCAATGTCGTCATAAGA+TGG + chr5.4:10606755-10606774 MS.gene010368:CDS 35.0%
TGAATACCATTTCTCTTGGT+AGG - chr5.4:10606637-10606656 None:intergenic 35.0%
TGAGTGAATACCATTTCTCT+TGG - chr5.4:10606641-10606660 None:intergenic 35.0%
TGATACTTGAATCTCAATGG+AGG + chr5.4:10607228-10607247 MS.gene010368:CDS 35.0%
TGATGAGGTTAATGGTTTCT+TGG - chr5.4:10606611-10606630 None:intergenic 35.0%
! TGATGAAACTGATGATGAAG+AGG + chr5.4:10607049-10607068 MS.gene010368:CDS 35.0%
!! GATTCTATTGGAAAGGCATT+TGG + chr5.4:10607257-10607276 MS.gene010368:CDS 35.0%
!! TGGAGGATTGTTGATTCTAT+TGG + chr5.4:10607245-10607264 MS.gene010368:CDS 35.0%
AGATGATGAAGGAAACAGAG+AGG + chr5.4:10607171-10607190 MS.gene010368:CDS 40.0%
CTTATGACGACATTGAAGAG+CGG - chr5.4:10606754-10606773 None:intergenic 40.0%
GAGGTTAATGGTTTCTTGGT+GGG - chr5.4:10606607-10606626 None:intergenic 40.0%
GTAGGATCTGATGAGGTTAA+TGG - chr5.4:10606619-10606638 None:intergenic 40.0%
TCAGATCCTACCAAGAGAAA+TGG + chr5.4:10606628-10606647 MS.gene010368:CDS 40.0%
TGAGGTTAATGGTTTCTTGG+TGG - chr5.4:10606608-10606627 None:intergenic 40.0%
TTGAGGTTTCTTTGTGGGAA+GGG - chr5.4:10606571-10606590 None:intergenic 40.0%
! AGTTGAGAAGTTTTGCAGAG+AGG + chr5.4:10607120-10607139 MS.gene010368:CDS 40.0%
! AGTTTTGCAGAGAGGATTGT+AGG + chr5.4:10607128-10607147 MS.gene010368:CDS 40.0%
! ATGGGTTGAGGTTTCTTTGT+GGG - chr5.4:10606576-10606595 None:intergenic 40.0%
! CGTTGTTGTCTTGTTACTGT+TGG - chr5.4:10607029-10607048 None:intergenic 40.0%
! GTTTTGCAGAGAGGATTGTA+GGG + chr5.4:10607129-10607148 MS.gene010368:CDS 40.0%
! TGAAAAAGCATAGTGCTGCT+AGG + chr5.4:10606961-10606980 MS.gene010368:CDS 40.0%
! TGAAACTGATGATGAAGAGG+AGG + chr5.4:10607052-10607071 MS.gene010368:CDS 40.0%
!! AATGGGTTGAGGTTTCTTTG+TGG - chr5.4:10606577-10606596 None:intergenic 40.0%
!! GTCTTGTTACTGTTGGTTTC+TGG - chr5.4:10607022-10607041 None:intergenic 40.0%
!!! ATTGAAGAGCGGTTTTGGAA+GGG - chr5.4:10606743-10606762 None:intergenic 40.0%
AGAAACCTCAACCCATTCCA+TGG + chr5.4:10606580-10606599 MS.gene010368:CDS 45.0%
CATGAGACGAAAATACTGCC+AGG - chr5.4:10606853-10606872 None:intergenic 45.0%
CTCATGGATGATCTTGAACG+CGG + chr5.4:10606867-10606886 MS.gene010368:CDS 45.0%
GTTGAGGTTTCTTTGTGGGA+AGG - chr5.4:10606572-10606591 None:intergenic 45.0%
TCTCTTGGTAGGATCTGATG+AGG - chr5.4:10606626-10606645 None:intergenic 45.0%
! AACTGATGATGAAGAGGAGG+AGG + chr5.4:10607055-10607074 MS.gene010368:CDS 45.0%
! AAGAGTGAGTTCTGGTGAAG+CGG - chr5.4:10606547-10606566 None:intergenic 45.0%
! ACGACATTGAAGAGCGGTTT+TGG - chr5.4:10606748-10606767 None:intergenic 45.0%
! ATAGGGTGCTTCTGAGAAAG+AGG - chr5.4:10606915-10606934 None:intergenic 45.0%
! ATAGTGCTGCTAGGTCTGTT+AGG + chr5.4:10606970-10606989 MS.gene010368:CDS 45.0%
! CTGGCAGTATTTTCGTCTCA+TGG + chr5.4:10606851-10606870 MS.gene010368:CDS 45.0%
!! TGAGGCGGTGTTTTTCAGAT+CGG - chr5.4:10606800-10606819 None:intergenic 45.0%
!!! CATTGAAGAGCGGTTTTGGA+AGG - chr5.4:10606744-10606763 None:intergenic 45.0%
CTGCCAGGATCGAGAAGAAA+CGG - chr5.4:10606838-10606857 None:intergenic 50.0%
CTGCTAGGTCTGTTAGGTCA+AGG + chr5.4:10606976-10606995 MS.gene010368:CDS 50.0%
GATGGCGAAGAGTTTGACAG+CGG + chr5.4:10607097-10607116 MS.gene010368:CDS 50.0%
GGAAGGGAAAGAGTGAGTTC+TGG - chr5.4:10606555-10606574 None:intergenic 50.0%
TGCAGAGAGGATTGTAGGGT+TGG + chr5.4:10607133-10607152 MS.gene010368:CDS 50.0%
! ATGGTTTCTTGGTGGGTCCA+TGG - chr5.4:10606600-10606619 None:intergenic 50.0%
! CTTCTGAGAAAGAGGTCGGA+CGG - chr5.4:10606907-10606926 None:intergenic 50.0%
! GGTGCTTCTGAGAAAGAGGT+CGG - chr5.4:10606911-10606930 None:intergenic 50.0%
! TCTTGGTGGGTCCATGGAAT+GGG - chr5.4:10606594-10606613 None:intergenic 50.0%
! TTCTTGGTGGGTCCATGGAA+TGG - chr5.4:10606595-10606614 None:intergenic 50.0%
! TTTCAGATCGGTGGCGTTGA+CGG - chr5.4:10606788-10606807 None:intergenic 50.0%
AGAAACGGCGGAGGAAGTAG+CGG - chr5.4:10606823-10606842 None:intergenic 55.0%
AGACGAAGAGGAGGGAGTGA+TGG + chr5.4:10607079-10607098 MS.gene010368:CDS 55.0%
AGAGGAGGAGGAAGACGAAG+AGG + chr5.4:10607067-10607086 MS.gene010368:CDS 55.0%
CCAGGATCGAGAAGAAACGG+CGG - chr5.4:10606835-10606854 None:intergenic 55.0%
CCTATCTCCGACGATAGCGA+CGG + chr5.4:10606930-10606949 MS.gene010368:CDS 55.0%
CGGCAGTCAATGGGAAGAAG+TGG + chr5.4:10606677-10606696 MS.gene010368:CDS 55.0%
CGTCGCTATCGTCGGAGATA+GGG - chr5.4:10606932-10606951 None:intergenic 55.0%
GAAAGAGGTCGGACGGAGAT+AGG - chr5.4:10606900-10606919 None:intergenic 55.0%
GAGGAGGAAGACGAAGAGGA+GGG + chr5.4:10607071-10607090 MS.gene010368:CDS 55.0%
GGTCGGACGGAGATAGGTAA+AGG - chr5.4:10606894-10606913 None:intergenic 55.0%
GTAATCGTAGCCGCAACGAG+CGG - chr5.4:10606715-10606734 None:intergenic 55.0%
! AGTGAGTTCTGGTGAAGCGG+TGG - chr5.4:10606544-10606563 None:intergenic 55.0%
! CTTCTTCCCATTGACTGCCG+CGG - chr5.4:10606677-10606696 None:intergenic 55.0%
!! GGCGGTGTTTTTCAGATCGG+TGG - chr5.4:10606797-10606816 None:intergenic 55.0%
!! TGGGTCCATGGAATGGGTTG+AGG - chr5.4:10606588-10606607 None:intergenic 55.0%
!!! GAAGAGCGGTTTTGGAAGGG+TGG - chr5.4:10606740-10606759 None:intergenic 55.0%
CCGCCGTTTCTTCTCGATCC+TGG + chr5.4:10606832-10606851 MS.gene010368:CDS 60.0%
CCGTCGCTATCGTCGGAGAT+AGG - chr5.4:10606933-10606952 None:intergenic 60.0%
CCTCTCACCGTCGCTATCGT+CGG - chr5.4:10606940-10606959 None:intergenic 60.0%
CGACGATAGCGACGGTGAGA+GGG + chr5.4:10606938-10606957 MS.gene010368:CDS 60.0%
GGAGGAGGAAGACGAAGAGG+AGG + chr5.4:10607070-10607089 MS.gene010368:CDS 60.0%
GGATCGAGAAGAAACGGCGG+AGG - chr5.4:10606832-10606851 None:intergenic 60.0%
GTAGTCGTAGCCGCTCGTTG+CGG + chr5.4:10606702-10606721 MS.gene010368:CDS 60.0%
CCCACTCCGCGGCAGTCAAT+GGG + chr5.4:10606668-10606687 MS.gene010368:CDS 65.0%
CCGACGATAGCGACGGTGAG+AGG + chr5.4:10606937-10606956 MS.gene010368:CDS 65.0%
CGGAGGAAGTAGCGGTGAGG+CGG - chr5.4:10606815-10606834 None:intergenic 65.0%
! CCCATTGACTGCCGCGGAGT+GGG - chr5.4:10606671-10606690 None:intergenic 65.0%
! TCCCATTGACTGCCGCGGAG+TGG - chr5.4:10606672-10606691 None:intergenic 65.0%
CGGCGGAGGAAGTAGCGGTG+AGG - chr5.4:10606818-10606837 None:intergenic 70.0%
CTCAAACGGGGCCCACTCCG+CGG + chr5.4:10606657-10606676 MS.gene010368:CDS 70.0%
GCCCACTCCGCGGCAGTCAA+TGG + chr5.4:10606667-10606686 MS.gene010368:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 10606537 10607289 10606537 ID=MS.gene010368
chr5.4 mRNA 10606537 10607289 10606537 ID=MS.gene010368.t1;Parent=MS.gene010368
chr5.4 exon 10606537 10607289 10606537 ID=MS.gene010368.t1.exon1;Parent=MS.gene010368.t1
chr5.4 CDS 10606537 10607289 10606537 ID=cds.MS.gene010368.t1;Parent=MS.gene010368.t1
Gene Sequence

>MS.gene010368

ATGGCCACCGCTTCACCAGAACTCACTCTTTCCCTTCCCACAAAGAAACCTCAACCCATTCCATGGACCCACCAAGAAACCATTAACCTCATCAGATCCTACCAAGAGAAATGGTATTCACTCAAACGGGGCCCACTCCGCGGCAGTCAATGGGAAGAAGTGGCTGTAGTCGTAGCCGCTCGTTGCGGCTACGATTACAACCACCCTTCCAAAACCGCTCTTCAATGTCGTCATAAGATGGAAAAACTCCGTCAACGCCACCGATCTGAAAAACACCGCCTCACCGCTACTTCCTCCGCCGTTTCTTCTCGATCCTGGCAGTATTTTCGTCTCATGGATGATCTTGAACGCGGTCCTTTACCTATCTCCGTCCGACCTCTTTCTCAGAAGCACCCTATCTCCGACGATAGCGACGGTGAGAGGGTGAAAAAGCATAGTGCTGCTAGGTCTGTTAGGTCAAGGAGTATTCATACTGTTCTTAACCAGAAACCAACAGTAACAAGACAACAACGTGATGAAACTGATGATGAAGAGGAGGAGGAAGACGAAGAGGAGGGAGTGATGGCGAAGAGTTTGACAGCGGAGTTGAGAAGTTTTGCAGAGAGGATTGTAGGGTTGGAAAATAAGAAGATGGAGATGATGAAGGAAACAGAGAGGTTTAGATTAGAGATGGAGAATAAGAGGATTCGTATGATACTTGAATCTCAATGGAGGATTGTTGATTCTATTGGAAAGGCATTTGGGTCTTCTTAG
Protein sequence

>MS.gene010368.t1

MATASPELTLSLPTKKPQPIPWTHQETINLIRSYQEKWYSLKRGPLRGSQWEEVAVVVAARCGYDYNHPSKTALQCRHKMEKLRQRHRSEKHRLTATSSAVSSRSWQYFRLMDDLERGPLPISVRPLSQKHPISDDSDGERVKKHSAARSVRSRSIHTVLNQKPTVTRQQRDETDDEEEEEDEEEGVMAKSLTAELRSFAERIVGLENKKMEMMKETERFRLEMENKRIRMILESQWRIVDSIGKAFGSS