AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010397

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010397.t1 MTR_5g017980 95.062 324 16 0 2 325 3 326 0.0 571
MS.gene010397.t1 MTR_5g017980 94.753 324 16 1 2 325 3 325 0.0 564
MS.gene010397.t1 MTR_5g017980 94.753 324 15 2 2 325 3 324 0.0 560
MS.gene010397.t1 MTR_4g086835 39.268 410 149 13 5 324 8 407 1.91e-65 212
MS.gene010397.t1 MTR_4g086835 39.024 410 149 14 5 324 8 406 5.71e-63 206
MS.gene010397.t1 MTR_4g113140 35.505 307 139 9 9 263 6 305 3.32e-40 146
MS.gene010397.t1 MTR_5g054300 36.398 261 102 8 39 253 17 259 7.19e-36 133
MS.gene010397.t1 MTR_1g093080 33.333 330 143 14 34 293 2 324 1.18e-35 133
MS.gene010397.t1 MTR_1g093080 34.767 279 117 12 34 254 2 273 8.97e-29 112
MS.gene010397.t1 MTR_3g106220 39.200 125 64 5 191 306 197 318 2.18e-14 74.3
MS.gene010397.t1 MTR_2g027860 55.714 70 30 1 192 261 49 117 2.69e-14 73.2
MS.gene010397.t1 MTR_7g088070 63.636 55 20 0 192 246 50 104 3.66e-14 72.0
MS.gene010397.t1 MTR_7g088070 63.636 55 20 0 192 246 50 104 3.91e-14 72.0
MS.gene010397.t1 MTR_4g121020 57.377 61 25 1 191 251 214 273 4.99e-14 73.2
MS.gene010397.t1 MTR_4g121020 57.377 61 25 1 191 251 214 273 5.09e-14 73.2
MS.gene010397.t1 MTR_5g041350 60.000 55 22 0 192 246 15 69 5.11e-14 72.4
MS.gene010397.t1 MTR_4g121020 57.377 61 25 1 191 251 110 169 5.59e-14 73.2
MS.gene010397.t1 MTR_1g090670 58.621 58 24 0 189 246 41 98 1.79e-13 70.1
MS.gene010397.t1 MTR_4g081710 42.697 89 50 1 192 279 17 105 2.87e-13 70.1
MS.gene010397.t1 MTR_1g032570 48.649 74 37 1 189 262 128 200 3.12e-13 70.9
MS.gene010397.t1 MTR_1g032570 48.649 74 37 1 189 262 197 269 3.46e-13 70.9
MS.gene010397.t1 MTR_1g013170 50.769 65 31 1 187 251 186 249 3.78e-13 70.5
MS.gene010397.t1 MTR_2g034960 50.000 72 35 1 189 260 208 278 3.82e-13 70.5
MS.gene010397.t1 MTR_1g053830 50.877 57 28 0 190 246 16 72 4.24e-13 65.1
MS.gene010397.t1 MTR_5g014040 42.222 90 46 3 163 251 285 369 4.66e-13 70.1
MS.gene010397.t1 MTR_2g027800 60.000 55 22 0 192 246 39 93 5.77e-13 68.9
MS.gene010397.t1 MTR_7g089010 51.667 60 28 1 191 250 90 148 5.83e-13 68.9
MS.gene010397.t1 MTR_1g080330 53.731 67 30 1 189 255 172 237 6.32e-13 68.6
MS.gene010397.t1 MTR_1g080330 53.731 67 30 1 189 255 172 237 7.04e-13 69.3
MS.gene010397.t1 MTR_2g027800 60.000 55 22 0 192 246 39 93 8.25e-13 68.6
MS.gene010397.t1 MTR_7g098250 52.239 67 31 1 189 255 259 324 1.63e-12 68.6
MS.gene010397.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 1.70e-12 67.4
MS.gene010397.t1 MTR_6g444980 60.000 55 22 0 192 246 35 89 1.83e-12 67.8
MS.gene010397.t1 MTR_6g444980 60.000 55 22 0 192 246 35 89 2.13e-12 67.4
MS.gene010397.t1 MTR_2g450070 50.000 62 30 1 189 250 210 270 2.46e-12 68.2
MS.gene010397.t1 MTR_2g450070 50.000 62 30 1 189 250 210 270 2.75e-12 68.2
MS.gene010397.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 2.80e-12 67.0
MS.gene010397.t1 MTR_3g064840 46.988 83 40 1 163 245 249 327 2.84e-12 67.8
MS.gene010397.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 3.00e-12 67.0
MS.gene010397.t1 MTR_2g086450 38.679 106 50 2 141 246 9 99 4.08e-12 67.0
MS.gene010397.t1 MTR_7g115530 59.259 54 22 0 192 245 218 271 4.45e-12 67.0
MS.gene010397.t1 MTR_5g027440 47.222 72 36 1 192 261 25 96 5.60e-12 65.9
MS.gene010397.t1 MTR_4g064730 45.312 64 34 1 188 251 127 189 7.64e-12 65.5
MS.gene010397.t1 MTR_1g053835 54.386 57 26 0 190 246 256 312 8.69e-12 66.2
MS.gene010397.t1 MTR_6g071625 37.815 119 57 3 158 270 175 282 8.78e-12 65.9
MS.gene010397.t1 MTR_6g032990 52.727 55 26 0 192 246 42 96 1.45e-11 65.5
MS.gene010397.t1 MTR_1g112370 59.184 49 20 0 197 245 27 75 1.48e-11 65.1
MS.gene010397.t1 MTR_7g093030 53.571 56 26 0 190 245 210 265 2.38e-11 63.9
MS.gene010397.t1 MTR_5g029470 31.061 132 78 2 153 284 184 302 2.69e-11 64.7
MS.gene010397.t1 MTR_4g131580 50.000 60 29 1 191 250 199 257 3.05e-11 64.7
MS.gene010397.t1 MTR_7g117705 45.714 70 37 1 191 260 190 258 3.98e-11 64.3
MS.gene010397.t1 MTR_7g093030 53.571 56 26 0 190 245 210 265 5.00e-11 63.5
MS.gene010397.t1 MTR_4g098870 43.939 66 36 1 186 251 343 407 5.13e-11 63.9
MS.gene010397.t1 MTR_4g098870 43.939 66 36 1 186 251 304 368 5.22e-11 63.9
MS.gene010397.t1 MTR_4g098870 43.939 66 36 1 186 251 324 388 5.23e-11 63.9
MS.gene010397.t1 MTR_8g066310 50.820 61 30 0 185 245 166 226 5.93e-11 63.2
MS.gene010397.t1 MTR_4g098870 43.939 66 36 1 186 251 304 368 6.57e-11 63.5
MS.gene010397.t1 MTR_4g098870 43.939 66 36 1 186 251 324 388 6.76e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010397.t1 AT4G37180 40.055 362 156 11 6 329 12 350 1.86e-60 197
MS.gene010397.t1 AT4G37180 39.295 369 156 12 6 329 12 357 1.41e-57 190
MS.gene010397.t1 AT4G37180 43.985 266 103 8 6 242 12 260 1.42e-51 172
MS.gene010397.t1 AT2G03500 38.112 286 133 8 9 250 6 291 1.68e-42 152
MS.gene010397.t1 AT1G68670 35.461 282 118 7 31 256 6 279 2.70e-40 145
MS.gene010397.t1 AT1G25550 35.636 275 116 7 31 256 10 272 4.39e-40 144
MS.gene010397.t1 AT1G49560 56.154 130 37 4 180 290 171 299 6.63e-35 130
MS.gene010397.t1 AT1G25550 34.483 261 111 6 31 242 10 259 1.10e-33 125
MS.gene010397.t1 AT1G13300 85.000 60 9 0 191 250 180 239 3.89e-29 115
MS.gene010397.t1 AT1G49560 70.833 72 12 1 180 242 171 242 4.10e-26 105
MS.gene010397.t1 AT3G25790 81.818 55 10 0 196 250 200 254 1.84e-25 105
MS.gene010397.t1 AT3G25790 80.851 47 9 0 196 242 200 246 2.37e-20 89.4
MS.gene010397.t1 AT2G01060 54.930 71 31 1 185 255 8 77 2.46e-14 72.8
MS.gene010397.t1 AT2G20400 44.828 87 47 1 192 278 231 316 2.95e-14 73.6
MS.gene010397.t1 AT2G20400 46.512 86 43 2 192 277 231 313 1.20e-13 71.6
MS.gene010397.t1 AT2G06020 50.000 64 32 0 183 246 77 140 1.44e-13 70.9
MS.gene010397.t1 AT4G13640 61.818 55 21 0 192 246 37 91 1.60e-13 70.5
MS.gene010397.t1 AT4G13640 61.818 55 21 0 192 246 37 91 1.63e-13 70.5
MS.gene010397.t1 AT5G05090 51.667 60 28 1 191 250 80 138 2.07e-13 69.7
MS.gene010397.t1 AT3G10760 51.667 60 28 1 191 250 104 162 3.16e-13 70.1
MS.gene010397.t1 AT3G24120 60.000 55 22 0 192 246 41 95 3.25e-13 69.7
MS.gene010397.t1 AT3G24120 60.000 55 22 0 192 246 41 95 3.50e-13 69.3
MS.gene010397.t1 AT5G59570 46.875 64 33 1 188 251 137 199 3.92e-13 69.3
MS.gene010397.t1 AT5G59570 46.875 64 33 1 188 251 137 199 3.92e-13 69.3
MS.gene010397.t1 AT2G40970 51.667 60 28 1 191 250 104 162 6.31e-13 68.2
MS.gene010397.t1 AT3G12730 58.182 55 23 0 192 246 23 77 8.12e-13 67.4
MS.gene010397.t1 AT3G16857 54.098 61 27 1 191 251 166 225 9.19e-13 69.3
MS.gene010397.t1 AT3G16857 54.098 61 27 1 191 251 236 295 1.06e-12 69.3
MS.gene010397.t1 AT3G16857 54.098 61 27 1 191 251 236 295 1.18e-12 69.3
MS.gene010397.t1 AT5G58080 49.206 63 31 1 189 251 191 252 1.36e-12 68.9
MS.gene010397.t1 AT3G04030 56.364 55 24 0 192 246 45 99 2.28e-12 67.8
MS.gene010397.t1 AT3G04030 56.364 55 24 0 192 246 45 99 2.46e-12 67.8
MS.gene010397.t1 AT5G06800 54.545 55 25 0 192 246 192 246 2.71e-12 66.6
MS.gene010397.t1 AT1G79430 60.000 55 22 0 192 246 34 88 3.35e-12 67.0
MS.gene010397.t1 AT1G67710 50.746 67 33 0 185 251 112 178 3.50e-12 67.4
MS.gene010397.t1 AT1G67710 50.746 67 33 0 185 251 121 187 3.53e-12 67.4
MS.gene010397.t1 AT1G67710 50.746 67 33 0 185 251 186 252 4.02e-12 67.4
MS.gene010397.t1 AT5G18240 56.364 55 24 0 192 246 45 99 4.75e-12 67.0
MS.gene010397.t1 AT5G18240 56.364 55 24 0 192 246 45 99 4.75e-12 67.0
MS.gene010397.t1 AT3G13040 50.000 64 32 0 183 246 232 295 4.87e-12 67.0
MS.gene010397.t1 AT3G13040 50.000 64 32 0 183 246 232 295 4.87e-12 67.0
MS.gene010397.t1 AT3G13040 50.000 64 32 0 183 246 232 295 4.87e-12 67.0
MS.gene010397.t1 AT5G18240 56.364 55 24 0 192 246 45 99 5.42e-12 66.6
MS.gene010397.t1 AT5G18240 56.364 55 24 0 192 246 45 99 5.55e-12 66.6
MS.gene010397.t1 AT5G18240 56.364 55 24 0 192 246 45 99 5.55e-12 66.6
MS.gene010397.t1 AT3G46640 47.541 61 31 1 191 251 143 202 5.66e-12 66.2
MS.gene010397.t1 AT3G46640 47.541 61 31 1 191 251 143 202 5.66e-12 66.2
MS.gene010397.t1 AT4G17695 41.237 97 47 2 177 272 148 235 5.73e-12 66.2
MS.gene010397.t1 AT3G04450 50.820 61 30 0 185 245 231 291 5.88e-12 65.9
MS.gene010397.t1 AT5G45580 56.364 55 24 0 192 246 7 61 6.34e-12 65.5
MS.gene010397.t1 AT5G45580 56.364 55 24 0 192 246 23 77 6.88e-12 65.5
MS.gene010397.t1 AT3G46640 47.541 61 31 1 191 251 143 202 7.52e-12 65.9
MS.gene010397.t1 AT5G06800 54.545 55 25 0 192 246 192 246 7.65e-12 66.2
MS.gene010397.t1 AT5G06800 54.545 55 25 0 192 246 192 246 7.87e-12 66.2
MS.gene010397.t1 AT1G32240 38.776 98 51 1 182 279 203 291 8.25e-12 65.9
MS.gene010397.t1 AT1G32240 38.776 98 51 1 182 279 203 291 1.02e-11 65.9
MS.gene010397.t1 AT3G04450 50.820 61 30 0 185 245 231 291 1.05e-11 65.9
MS.gene010397.t1 AT3G04450 50.820 61 30 0 185 245 191 251 1.22e-11 65.5
MS.gene010397.t1 AT3G04450 50.820 61 30 0 185 245 191 251 1.22e-11 65.5
MS.gene010397.t1 AT2G01760 50.000 62 30 1 190 251 226 286 1.51e-11 65.5
MS.gene010397.t1 AT2G01760 50.000 62 30 1 190 251 133 193 1.51e-11 64.7
MS.gene010397.t1 AT2G01760 50.000 62 30 1 190 251 198 258 1.57e-11 65.1
MS.gene010397.t1 AT1G69580 50.909 55 27 0 192 246 34 88 1.60e-11 65.1
MS.gene010397.t1 AT2G25180 46.377 69 36 1 191 259 194 261 1.63e-11 65.5
MS.gene010397.t1 AT1G69580 50.909 55 27 0 192 246 34 88 1.76e-11 64.7
MS.gene010397.t1 AT5G29000 52.632 57 27 0 189 245 228 284 2.11e-11 64.3
MS.gene010397.t1 AT5G16560 51.562 64 31 0 182 245 209 272 2.46e-11 64.7
MS.gene010397.t1 AT4G31920 41.892 74 42 1 191 264 182 254 2.48e-11 65.1
MS.gene010397.t1 AT4G28610 55.556 54 24 0 192 245 225 278 2.77e-11 64.7
MS.gene010397.t1 AT5G29000 52.632 57 27 0 189 245 185 241 4.52e-11 63.9
MS.gene010397.t1 AT5G29000 52.632 57 27 0 189 245 185 241 4.52e-11 63.9
MS.gene010397.t1 AT5G29000 52.632 57 27 0 189 245 228 284 4.61e-11 63.9
MS.gene010397.t1 AT5G42630 53.333 60 28 0 186 245 99 158 6.11e-11 62.0
MS.gene010397.t1 AT4G04605 53.571 56 26 0 190 245 12 67 9.28e-11 61.2

Find 72 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATGGTGGAGGGGTATTTA+TGG 0.172244 5.4:-10950535 MS.gene010397:CDS
CAGCCACTCCTAAGCAAATT+AGG 0.199099 5.4:-10949744 MS.gene010397:CDS
AAAATTAGTTACCATCATTA+AGG 0.265474 5.4:+10950921 None:intergenic
CCAGCACCTCCTTTGTTTCC+TGG 0.269810 5.4:+10950344 None:intergenic
AAAAGTTGGGTTGATGAAAA+TGG 0.274484 5.4:-10950782 MS.gene010397:CDS
ATTCCCAGTTTCGTCAATTC+AGG 0.284606 5.4:-10949513 MS.gene010397:CDS
AGCAATCAGATTGCAGGAAT+TGG 0.302511 5.4:-10949319 MS.gene010397:CDS
CTCCTTGGTGGATCTGTTCA+AGG 0.312173 5.4:-10949383 MS.gene010397:CDS
GCCTCCTGAATTGACGAAAC+TGG 0.313674 5.4:+10949509 None:intergenic
AATGGTGGAGGGGTATTTAT+GGG 0.317942 5.4:-10950534 MS.gene010397:CDS
AAGAGTGGTGATGGTAGTTC+TGG 0.342533 5.4:-10950432 MS.gene010397:CDS
AACCTTTGCAGCAGAGTTCC+AGG 0.347695 5.4:-10950362 MS.gene010397:CDS
ACAAGCTAAGAGCTTTAGTT+TGG 0.377820 5.4:-10950481 MS.gene010397:CDS
ACTGTGATAATAAGAAGAAT+TGG 0.383175 5.4:-10950744 MS.gene010397:CDS
AGCCACTCCTAAGCAAATTA+GGG 0.392450 5.4:-10949743 MS.gene010397:CDS
CTCAGAGTCTGTTTCTCCAC+AGG 0.393005 5.4:-10949423 MS.gene010397:CDS
TGCAAAGGTTGCGGCGGCTG+AGG 0.403383 5.4:+10950375 None:intergenic
CATCCATACTGTTTCTTCCA+TGG 0.409764 5.4:+10949351 None:intergenic
TTGTGATTATGCAGAAGTAT+AGG 0.413901 5.4:-10949550 MS.gene010397:intron
CCTCTTACTCCTCTTCTCCT+TGG 0.417068 5.4:-10949398 MS.gene010397:CDS
AAGATGAGCAATCAGATTGC+AGG 0.425444 5.4:-10949325 MS.gene010397:CDS
TAAGGAGGAGTATGGAAAGA+GGG 0.427844 5.4:+10950939 None:intergenic
TAATAAGTTAGCCTTGTATA+TGG 0.434530 5.4:-10949486 MS.gene010397:CDS
ACCATCATTAAGGAGGAGTA+TGG 0.434982 5.4:+10950931 None:intergenic
AAGCCATGGAAGAAACAGTA+TGG 0.440126 5.4:-10949354 MS.gene010397:CDS
CTTACTCCTCTTCTCCTTGG+TGG 0.445651 5.4:-10949395 MS.gene010397:CDS
ACAATGAAGGTGATGATGAT+AGG 0.445952 5.4:-10950575 MS.gene010397:CDS
TATTGTCACTCACTTGCAAA+TGG 0.459590 5.4:+10949677 None:intergenic
TCCATACTCCTCCTTAATGA+TGG 0.469852 5.4:-10950932 MS.gene010397:intron
CCAAGGAGAAGAGGAGTAAG+AGG 0.473664 5.4:+10949398 None:intergenic
TTCCCTAATTTGCTTAGGAG+TGG 0.487933 5.4:+10949741 None:intergenic
TTAAGGAGGAGTATGGAAAG+AGG 0.491286 5.4:+10950938 None:intergenic
CAGGAATTGGAAAAGTGATC+AGG 0.500799 5.4:-10949306 MS.gene010397:CDS
ATTTAATGAAAATCTGTTGA+AGG 0.503957 5.4:-10950511 MS.gene010397:CDS
TTCCTGGAACTCTGCTGCAA+AGG 0.504800 5.4:+10950360 None:intergenic
GTTGAAGTGCATCAACAAAT+CGG 0.508453 5.4:+10950304 None:intergenic
TGATGACTTAGTTCATAGCT+TGG 0.508826 5.4:-10951002 MS.gene010397:CDS
CCTCCTGAATTGACGAAACT+GGG 0.520520 5.4:+10949510 None:intergenic
CCAGGAAACAAAGGAGGTGC+TGG 0.526510 5.4:-10950344 MS.gene010397:CDS
GTTCAAGGTCTTTCAAGCCA+TGG 0.531430 5.4:-10949368 MS.gene010397:CDS
GAACTCTGCTGCAAAGGTTG+CGG 0.536482 5.4:+10950366 None:intergenic
AGGGAAAAGATGCAAGTGGA+TGG 0.538779 5.4:-10949724 MS.gene010397:CDS
TCAGAGTCTGTTTCTCCACA+GGG 0.539092 5.4:-10949422 MS.gene010397:CDS
GGATGAGTTCAGCTCAGCTA+TGG 0.541177 5.4:-10950723 MS.gene010397:CDS
GGAAATTCAAAGAGTGGTGA+TGG 0.543852 5.4:-10950441 MS.gene010397:CDS
GTTGTTGAGGAAATGAGAAA+CGG 0.545414 5.4:+10951066 None:intergenic
TAATGAAAATCTGTTGAAGG+AGG 0.547757 5.4:-10950508 MS.gene010397:CDS
CTCTGCTGCAAAGGTTGCGG+CGG 0.547761 5.4:+10950369 None:intergenic
TGGTTTGAACAACGTTGTTG+AGG 0.552246 5.4:+10951053 None:intergenic
TGTGAAGAAGATACATCAAA+GGG 0.552624 5.4:-10949452 MS.gene010397:CDS
CCCAGTTTCGTCAATTCAGG+AGG 0.558843 5.4:-10949510 MS.gene010397:CDS
ACAAAGGAGGTGCTGGTCGT+CGG 0.559002 5.4:-10950337 MS.gene010397:CDS
CAACAGCTTGGTGGAGCACA+TGG 0.565013 5.4:-10950285 MS.gene010397:intron
GTTGATGCACTTCAACAGCT+TGG 0.565906 5.4:-10950297 MS.gene010397:CDS
ACAGAAGAGTAATGGTGGAG+GGG 0.566775 5.4:-10950544 MS.gene010397:CDS
ACAGATCCACCAAGGAGAAG+AGG 0.569987 5.4:+10949389 None:intergenic
GTGTTACAGAAGAGTAATGG+TGG 0.573520 5.4:-10950549 MS.gene010397:CDS
ATGTGAAGAAGATACATCAA+AGG 0.582691 5.4:-10949453 MS.gene010397:CDS
GATGCACTTCAACAGCTTGG+TGG 0.583631 5.4:-10950294 MS.gene010397:CDS
ATTAGTTACCATCATTAAGG+AGG 0.585189 5.4:+10950924 None:intergenic
AGCAGAGTTCCAGGAAACAA+AGG 0.588997 5.4:-10950353 MS.gene010397:CDS
ATTGTGTTACAGAAGAGTAA+TGG 0.592672 5.4:-10950552 MS.gene010397:CDS
TTACAGAAGAGTAATGGTGG+AGG 0.599891 5.4:-10950546 MS.gene010397:CDS
TACAGAAGAGTAATGGTGGA+GGG 0.606656 5.4:-10950545 MS.gene010397:CDS
AATTAGGGAAAAGATGCAAG+TGG 0.615575 5.4:-10949728 MS.gene010397:CDS
TTGATCTTGAGCCATATACA+AGG 0.616538 5.4:+10949475 None:intergenic
AGTCATGGAAATTCAAAGAG+TGG 0.617489 5.4:-10950447 MS.gene010397:CDS
TTGGTGTCTGAAGTGAGTCA+TGG 0.626831 5.4:-10950462 MS.gene010397:CDS
AAGTTTCTGAGACATGTCGT+TGG 0.646359 5.4:+10951033 None:intergenic
AGAGGAGTAAGAGGACCCTG+TGG 0.650364 5.4:+10949407 None:intergenic
AGAGTTCCAGGAAACAAAGG+AGG 0.672803 5.4:-10950350 MS.gene010397:CDS
GACCTTGAACAGATCCACCA+AGG 0.687479 5.4:+10949381 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATCACAATTAAAAAAA+AGG + chr5.4:10949757-10949776 None:intergenic 10.0%
!! AAAATTAACAAAAAAGAAAA+AGG + chr5.4:10949546-10949565 None:intergenic 10.0%
!!! AATTTTCTAATTAATTTTTG+TGG - chr5.4:10949497-10949516 MS.gene010397:CDS 10.0%
!! AAAATTAGTTACCATCATTA+AGG + chr5.4:10949483-10949502 None:intergenic 20.0%
!! AATGATGAAAAAGAAAAAGT+TGG - chr5.4:10949605-10949624 MS.gene010397:intron 20.0%
!! ATGATGAAAAAGAAAAAGTT+GGG - chr5.4:10949606-10949625 MS.gene010397:intron 20.0%
!! ATTTAATGAAAATCTGTTGA+AGG - chr5.4:10949890-10949909 MS.gene010397:intron 20.0%
!!! AGTTCTAAGTTATAACAAAT+AGG + chr5.4:10950532-10950551 None:intergenic 20.0%
! ACTAATAATCGTATTAACCA+TGG + chr5.4:10950773-10950792 None:intergenic 25.0%
! ACTGTGATAATAAGAAGAAT+TGG - chr5.4:10949657-10949676 MS.gene010397:intron 25.0%
! AGATTACATGACATGTTATT+AGG - chr5.4:10950492-10950511 MS.gene010397:CDS 25.0%
! GATTACATGACATGTTATTA+GGG - chr5.4:10950493-10950512 MS.gene010397:CDS 25.0%
! TACAAAAAAGAAAACCAGTT+AGG + chr5.4:10950636-10950655 None:intergenic 25.0%
! TAGAACTGTTTGATTAAGAT+TGG - chr5.4:10950545-10950564 MS.gene010397:CDS 25.0%
!! ATGGACTAATCAAACAAAAA+CGG - chr5.4:10949697-10949716 MS.gene010397:CDS 25.0%
!! ATGTTATTAGGGATTAGTAT+AGG - chr5.4:10950504-10950523 MS.gene010397:CDS 25.0%
!! CAATTTGGAATTGAAATTGT+GGG - chr5.4:10950261-10950280 MS.gene010397:intron 25.0%
!! TCTTAGTGTCTATTTTGTAT+TGG - chr5.4:10950215-10950234 MS.gene010397:intron 25.0%
!! TGTCTATTTTGTATTGGATT+TGG - chr5.4:10950221-10950240 MS.gene010397:intron 25.0%
!!! TAATAAGTTAGCCTTGTATA+TGG - chr5.4:10950915-10950934 MS.gene010397:intron 25.0%
!!! TTGTGGTATTTCTTGTTTTT+TGG - chr5.4:10949514-10949533 MS.gene010397:CDS 25.0%
!!! TTTTTTTGGTAGAACAATGA+AGG - chr5.4:10949813-10949832 MS.gene010397:intron 25.0%
AAAAAAATTTGACCGTACAC+AGG - chr5.4:10950590-10950609 MS.gene010397:intron 30.0%
AACTGTTTGATTAAGATTGG+AGG - chr5.4:10950548-10950567 MS.gene010397:CDS 30.0%
AATCAATCTTAGTCGTTGTA+TGG - chr5.4:10950352-10950371 MS.gene010397:CDS 30.0%
ATAATCGTATTAACCATGGA+TGG + chr5.4:10950769-10950788 None:intergenic 30.0%
ATGTGAAGAAGATACATCAA+AGG - chr5.4:10950948-10950967 MS.gene010397:CDS 30.0%
ATTAGTTACCATCATTAAGG+AGG + chr5.4:10949480-10949499 None:intergenic 30.0%
ATTGTGTTACAGAAGAGTAA+TGG - chr5.4:10949849-10949868 MS.gene010397:intron 30.0%
CCACAATTTCAATTCCAAAT+TGG + chr5.4:10950263-10950282 None:intergenic 30.0%
GCTCTGATTTATTATGGTAT+AGG - chr5.4:10950187-10950206 MS.gene010397:intron 30.0%
TAATGAAAATCTGTTGAAGG+AGG - chr5.4:10949893-10949912 MS.gene010397:intron 30.0%
TGTGAAGAAGATACATCAAA+GGG - chr5.4:10950949-10950968 MS.gene010397:CDS 30.0%
! AGTGTTGCTCTGATTTATTA+TGG - chr5.4:10950181-10950200 MS.gene010397:intron 30.0%
! ATAGAGTTGATTCTCCAATT+TGG - chr5.4:10950246-10950265 MS.gene010397:intron 30.0%
! CCAATTTGGAATTGAAATTG+TGG - chr5.4:10950260-10950279 MS.gene010397:intron 30.0%
! TTGTGATTATGCAGAAGTAT+AGG - chr5.4:10950851-10950870 MS.gene010397:intron 30.0%
!! AAAAGTTGGGTTGATGAAAA+TGG - chr5.4:10949619-10949638 MS.gene010397:intron 30.0%
!!! ATCTTTTCCCTAATTTGCTT+AGG + chr5.4:10950668-10950687 None:intergenic 30.0%
!!! TTTTTTTGCAATCAGCAAGA+TGG + chr5.4:10950577-10950596 None:intergenic 30.0%
AATTAGGGAAAAGATGCAAG+TGG - chr5.4:10950673-10950692 MS.gene010397:intron 35.0%
AGTCATGGAAATTCAAAGAG+TGG - chr5.4:10949954-10949973 MS.gene010397:intron 35.0%
GTTGAAGTGCATCAACAAAT+CGG + chr5.4:10950100-10950119 None:intergenic 35.0%
GTTGTTGAGGAAATGAGAAA+CGG + chr5.4:10949338-10949357 None:intergenic 35.0%
TATTGTCACTCACTTGCAAA+TGG + chr5.4:10950727-10950746 None:intergenic 35.0%
TGATGACTTAGTTCATAGCT+TGG - chr5.4:10949399-10949418 MS.gene010397:CDS 35.0%
TTGATCTTGAGCCATATACA+AGG + chr5.4:10950929-10950948 None:intergenic 35.0%
! ACAAGCTAAGAGCTTTAGTT+TGG - chr5.4:10949920-10949939 MS.gene010397:intron 35.0%
! ACAATGAAGGTGATGATGAT+AGG - chr5.4:10949826-10949845 MS.gene010397:intron 35.0%
! AGGAAATGAGAAACGGTTTT+TGG + chr5.4:10949331-10949350 None:intergenic 35.0%
! GAAATTGTGGGTCTTGTATT+GGG - chr5.4:10950273-10950292 MS.gene010397:intron 35.0%
! TGAAATTGTGGGTCTTGTAT+TGG - chr5.4:10950272-10950291 MS.gene010397:intron 35.0%
AAGATGAGCAATCAGATTGC+AGG - chr5.4:10951076-10951095 MS.gene010397:CDS 40.0%
AAGCCATGGAAGAAACAGTA+TGG - chr5.4:10951047-10951066 MS.gene010397:CDS 40.0%
AATCTGGATTACCTGCAGTT+AGG - chr5.4:10950306-10950325 MS.gene010397:CDS 40.0%
ACCATCATTAAGGAGGAGTA+TGG + chr5.4:10949473-10949492 None:intergenic 40.0%
ACCTGCAGTTAGGAAATATG+TGG - chr5.4:10950316-10950335 MS.gene010397:CDS 40.0%
AGCAATCAGATTGCAGGAAT+TGG - chr5.4:10951082-10951101 MS.gene010397:CDS 40.0%
AGCCACTCCTAAGCAAATTA+GGG - chr5.4:10950658-10950677 MS.gene010397:intron 40.0%
ATTCCCAGTTTCGTCAATTC+AGG - chr5.4:10950888-10950907 MS.gene010397:intron 40.0%
CAGGAATTGGAAAAGTGATC+AGG - chr5.4:10951095-10951114 MS.gene010397:CDS 40.0%
CGTATTAACCATGGATGGTT+TGG + chr5.4:10950764-10950783 None:intergenic 40.0%
GAATCCCACTCTTGTGTATA+GGG - chr5.4:10950465-10950484 MS.gene010397:CDS 40.0%
GGAAATTCAAAGAGTGGTGA+TGG - chr5.4:10949960-10949979 MS.gene010397:intron 40.0%
GTGTTACAGAAGAGTAATGG+TGG - chr5.4:10949852-10949871 MS.gene010397:intron 40.0%
GTTGTAAGATGCAGATCAGA+TGG + chr5.4:10950428-10950447 None:intergenic 40.0%
TCCACATATTTCCTAACTGC+AGG + chr5.4:10950320-10950339 None:intergenic 40.0%
TCCATACTCCTCCTTAATGA+TGG - chr5.4:10949469-10949488 MS.gene010397:CDS 40.0%
TGATCCCTATACACAAGAGT+GGG + chr5.4:10950472-10950491 None:intergenic 40.0%
TGGTTTGAACAACGTTGTTG+AGG + chr5.4:10949351-10949370 None:intergenic 40.0%
TTACAGAAGAGTAATGGTGG+AGG - chr5.4:10949855-10949874 MS.gene010397:intron 40.0%
TTGATCCCTATACACAAGAG+TGG + chr5.4:10950473-10950492 None:intergenic 40.0%
! CATCCATACTGTTTCTTCCA+TGG + chr5.4:10951053-10951072 None:intergenic 40.0%
! TAAGGAGGAGTATGGAAAGA+GGG + chr5.4:10949465-10949484 None:intergenic 40.0%
! TACAGAAGAGTAATGGTGGA+GGG - chr5.4:10949856-10949875 MS.gene010397:intron 40.0%
! TTAAGGAGGAGTATGGAAAG+AGG + chr5.4:10949466-10949485 None:intergenic 40.0%
!! AAGTTTCTGAGACATGTCGT+TGG + chr5.4:10949371-10949390 None:intergenic 40.0%
!! AATGGTGGAGGGGTATTTAT+GGG - chr5.4:10949867-10949886 MS.gene010397:intron 40.0%
!! TAATGGTGGAGGGGTATTTA+TGG - chr5.4:10949866-10949885 MS.gene010397:intron 40.0%
!! TTCCCTAATTTGCTTAGGAG+TGG + chr5.4:10950663-10950682 None:intergenic 40.0%
!!! AGAAACGGTTTTTGGAACAG+AGG + chr5.4:10949323-10949342 None:intergenic 40.0%
AAGATGCAGATCAGATGGCT+TGG + chr5.4:10950423-10950442 None:intergenic 45.0%
ACAACTGAGACAACCACTAC+AGG - chr5.4:10950443-10950462 MS.gene010397:CDS 45.0%
AGAGTTCCAGGAAACAAAGG+AGG - chr5.4:10950051-10950070 MS.gene010397:intron 45.0%
AGCAGAGTTCCAGGAAACAA+AGG - chr5.4:10950048-10950067 MS.gene010397:intron 45.0%
AGGGAAAAGATGCAAGTGGA+TGG - chr5.4:10950677-10950696 MS.gene010397:intron 45.0%
CAGCCACTCCTAAGCAAATT+AGG - chr5.4:10950657-10950676 MS.gene010397:intron 45.0%
CCTCCTGAATTGACGAAACT+GGG + chr5.4:10950894-10950913 None:intergenic 45.0%
CTCAAACTCCAAACCATCCA+TGG - chr5.4:10950753-10950772 MS.gene010397:CDS 45.0%
GGAATCCCACTCTTGTGTAT+AGG - chr5.4:10950464-10950483 MS.gene010397:CDS 45.0%
GTTCAAGGTCTTTCAAGCCA+TGG - chr5.4:10951033-10951052 MS.gene010397:CDS 45.0%
GTTGATGCACTTCAACAGCT+TGG - chr5.4:10950104-10950123 MS.gene010397:intron 45.0%
! ACAGAAGAGTAATGGTGGAG+GGG - chr5.4:10949857-10949876 MS.gene010397:intron 45.0%
! ATTGTGGGTCTTGTATTGGG+TGG - chr5.4:10950276-10950295 MS.gene010397:intron 45.0%
! TCAGAGTCTGTTTCTCCACA+GGG - chr5.4:10950979-10950998 MS.gene010397:CDS 45.0%
! TTGGTGTCTGAAGTGAGTCA+TGG - chr5.4:10949939-10949958 MS.gene010397:intron 45.0%
! TTGTGGGTCTTGTATTGGGT+GGG - chr5.4:10950277-10950296 MS.gene010397:intron 45.0%
!! AAGAGTGGTGATGGTAGTTC+TGG - chr5.4:10949969-10949988 MS.gene010397:intron 45.0%
!!! ATAAAAAAAAATGTTTTTTT+TGG - chr5.4:10949799-10949818 MS.gene010397:intron 5.0%
AACCTTTGCAGCAGAGTTCC+AGG - chr5.4:10950039-10950058 MS.gene010397:intron 50.0%
ACAGATCCACCAAGGAGAAG+AGG + chr5.4:10951015-10951034 None:intergenic 50.0%
AGGGTGTGTATGAGCCTAAC+TGG - chr5.4:10950619-10950638 MS.gene010397:intron 50.0%
CAAGAGTGGGATTCCTGTAG+TGG + chr5.4:10950459-10950478 None:intergenic 50.0%
CACCCTACATAGCCTGTGTA+CGG + chr5.4:10950605-10950624 None:intergenic 50.0%
CCAAGGAGAAGAGGAGTAAG+AGG + chr5.4:10951006-10951025 None:intergenic 50.0%
CCCAGTTTCGTCAATTCAGG+AGG - chr5.4:10950891-10950910 MS.gene010397:intron 50.0%
CCTCTTACTCCTCTTCTCCT+TGG - chr5.4:10951003-10951022 MS.gene010397:CDS 50.0%
CTTACTCCTCTTCTCCTTGG+TGG - chr5.4:10951006-10951025 MS.gene010397:CDS 50.0%
GAACTCTGCTGCAAAGGTTG+CGG + chr5.4:10950038-10950057 None:intergenic 50.0%
GACCGTACACAGGCTATGTA+GGG - chr5.4:10950600-10950619 MS.gene010397:intron 50.0%
GACCTTGAACAGATCCACCA+AGG + chr5.4:10951023-10951042 None:intergenic 50.0%
GATGCACTTCAACAGCTTGG+TGG - chr5.4:10950107-10950126 MS.gene010397:intron 50.0%
GCCTCCTGAATTGACGAAAC+TGG + chr5.4:10950895-10950914 None:intergenic 50.0%
GGATGAGTTCAGCTCAGCTA+TGG - chr5.4:10949678-10949697 MS.gene010397:intron 50.0%
TGACCGTACACAGGCTATGT+AGG - chr5.4:10950599-10950618 MS.gene010397:intron 50.0%
TTCCTGGAACTCTGCTGCAA+AGG + chr5.4:10950044-10950063 None:intergenic 50.0%
! CTCAGAGTCTGTTTCTCCAC+AGG - chr5.4:10950978-10950997 MS.gene010397:CDS 50.0%
! CTCCTTGGTGGATCTGTTCA+AGG - chr5.4:10951018-10951037 MS.gene010397:CDS 50.0%
! TGTGGGTCTTGTATTGGGTG+GGG - chr5.4:10950278-10950297 MS.gene010397:intron 50.0%
AGAGGAGTAAGAGGACCCTG+TGG + chr5.4:10950997-10951016 None:intergenic 55.0%
! CAACAGCTTGGTGGAGCACA+TGG - chr5.4:10950116-10950135 MS.gene010397:intron 55.0%
! CCAGCACCTCCTTTGTTTCC+TGG + chr5.4:10950060-10950079 None:intergenic 55.0%
! CCAGGAAACAAAGGAGGTGC+TGG - chr5.4:10950057-10950076 MS.gene010397:intron 55.0%
! GTGGGTCTTGTATTGGGTGG+GGG - chr5.4:10950279-10950298 MS.gene010397:intron 55.0%
! TGGGTCTTGTATTGGGTGGG+GGG - chr5.4:10950280-10950299 MS.gene010397:intron 55.0%
!! ACAAAGGAGGTGCTGGTCGT+CGG - chr5.4:10950064-10950083 MS.gene010397:intron 55.0%
ATTGGGTGGGGGGAGCAATC+TGG - chr5.4:10950290-10950309 MS.gene010397:CDS 60.0%
CTCTGCTGCAAAGGTTGCGG+CGG + chr5.4:10950035-10950054 None:intergenic 60.0%
TGCAAAGGTTGCGGCGGCTG+AGG + chr5.4:10950029-10950048 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 10949283 10951140 10949283 ID=MS.gene010397
chr5.4 mRNA 10949283 10951140 10949283 ID=MS.gene010397.t1;Parent=MS.gene010397
chr5.4 exon 10950933 10951140 10950933 ID=MS.gene010397.t1.exon1;Parent=MS.gene010397.t1
chr5.4 CDS 10950933 10951140 10950933 ID=cds.MS.gene010397.t1;Parent=MS.gene010397.t1
chr5.4 exon 10950705 10950832 10950705 ID=MS.gene010397.t1.exon2;Parent=MS.gene010397.t1
chr5.4 CDS 10950705 10950832 10950705 ID=cds.MS.gene010397.t1;Parent=MS.gene010397.t1
chr5.4 exon 10950286 10950598 10950286 ID=MS.gene010397.t1.exon3;Parent=MS.gene010397.t1
chr5.4 CDS 10950286 10950598 10950286 ID=cds.MS.gene010397.t1;Parent=MS.gene010397.t1
chr5.4 exon 10949690 10949766 10949690 ID=MS.gene010397.t1.exon4;Parent=MS.gene010397.t1
chr5.4 CDS 10949690 10949766 10949690 ID=cds.MS.gene010397.t1;Parent=MS.gene010397.t1
chr5.4 exon 10949283 10949558 10949283 ID=MS.gene010397.t1.exon5;Parent=MS.gene010397.t1
chr5.4 CDS 10949283 10949558 10949283 ID=cds.MS.gene010397.t1;Parent=MS.gene010397.t1
Gene Sequence

>MS.gene010397

ATGGAACAACAACAATTGAACTTAGATTTGACTTTAGCCTCTGTTCCAAAAACCGTTTCTCATTTCCTCAACAACGTTGTTCAAACCAACGACATGTCTCAGAAACTTTCAATGCTTGATGACTTAGTTCATAGCTTGGAAGAAGAAATGAAAAAAGTTCTTGCTTTCAAACGTGAACTCCCTCTTTCCATACTCCTCCTTAATGATGGTAACTAATTTTCTAATTAATTTTTGTGGTATTTCTTGTTTTTTGGATTTAACCTTTTTCTTTTTTGTTAATTTTTTATTTTTGCTGTTAATTGATGTAGCTATAGCAAGATTGAATGATGAAAAAGAAAAAGTTGGGTTGATGAAAATGGAAGATTTGAAAGAAAACTGTGATAATAAGAAGAATTGGATGAGTTCAGCTCAGCTATGGACTAATCAAACAAAAACGGTAATTTTAATATTTTTTTAGAAATTAGTAAATTACCTTTTTTTTAATTGTGATTTTTGTTTATTGAAGTGAGATTTGAGATAAAAAAAAATGTTTTTTTTGGTAGAACAATGAAGGTGATGATGATAGGATTGTGTTACAGAAGAGTAATGGTGGAGGGGTATTTATGGGATTTAATGAAAATCTGTTGAAGGAGGTTTCACAAGCTAAGAGCTTTAGTTTGGTGTCTGAAGTGAGTCATGGAAATTCAAAGAGTGGTGATGGTAGTTCTGGATCGTCTTTGTTGCGTGTTGAGATACAGAATCAACCTCAGCCGCCGCAACCTTTGCAGCAGAGTTCCAGGAAACAAAGGAGGTGCTGGTCGTCGGAGCTTCATCGCCGATTTGTTGATGCACTTCAACAGCTTGGTGGAGCACATGGTAAATTGTTTTTGTTCAGATTGATTGTTAATTAGTTGTGTTAAGTGTTGCTCTGATTTATTATGGTATAGGAATTTTCTTAGTGTCTATTTTGTATTGGATTTGGTAATAGAGTTGATTCTCCAATTTGGAATTGAAATTGTGGGTCTTGTATTGGGTGGGGGGAGCAATCTGGATTACCTGCAGTTAGGAAATATGTGGATTTAGTGTATTTAATCAATCTTAGTCGTTGTATGGATATCATACGATTTTATAGTTGTGTAAAATAGTTCAGCTTGAAATCCAAGCCATCTGATCTGCATCTTACAACTGAGACAACCACTACAGGAATCCCACTCTTGTGTATAGGGATCAAGATTACATGACATGTTATTAGGGATTAGTATAGGCACCTATTTGTTATAACTTAGAACTGTTTGATTAAGATTGGAGGTTTCCATCTTGCTGATTGCAAAAAAATTTGACCGTACACAGGCTATGTAGGGTGTGTATGAGCCTAACTGGTTTTCTTTTTTGTAGCAGCCACTCCTAAGCAAATTAGGGAAAAGATGCAAGTGGATGGATTAACAAATGATGAAGTGAAAAGCCATTTGCAAGTGAGTGACAATATTTCATCTCAAACTCCAAACCATCCATGGTTAATACGATTATTAGTTTCTTCGTATTAATTTGATTCTTTTGTTATGTCATGTCAAGCAATTGATGTAGCACATTTGTGATTATGCAGAAGTATAGGCTTCATGTTAGAAGATTCCCAGTTTCGTCAATTCAGGAGGCTAATAAGTTAGCCTTGTATATGGCTCAAGATCAATGTGAAGAAGATACATCAAAGGGAAACTTCTCAGAGTCTGTTTCTCCACAGGGTCCTCTTACTCCTCTTCTCCTTGGTGGATCTGTTCAAGGTCTTTCAAGCCATGGAAGAAACAGTATGGATGCTGAAGATGAGCAATCAGATTGCAGGAATTGGAAAAGTGATCAGGAGCAGCAGCATGAAGCTGAATAA
Protein sequence

>MS.gene010397.t1

MEQQQLNLDLTLASVPKTVSHFLNNVVQTNDMSQKLSMLDDLVHSLEEEMKKVLAFKRELPLSILLLNDAIARLNDEKEKVGLMKMEDLKENCDNKKNWMSSAQLWTNQTKTNNEGDDDRIVLQKSNGGGVFMGFNENLLKEVSQAKSFSLVSEVSHGNSKSGDGSSGSSLLRVEIQNQPQPPQPLQQSSRKQRRCWSSELHRRFVDALQQLGGAHAATPKQIREKMQVDGLTNDEVKSHLQKYRLHVRRFPVSSIQEANKLALYMAQDQCEEDTSKGNFSESVSPQGPLTPLLLGGSVQGLSSHGRNSMDAEDEQSDCRNWKSDQEQQHEAE