AlfalfaGEDB | MS.gene010405

M. sativa cultivar XinJiangDaYe / MS.gene010405

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010405.t1	XP_003589401.2	89.5	124	12	1	1	124	1	123	9.30E-53	216.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010405.t1	Q9SRH9	33.8	154	46	2	17	114	87	240	1.2e-13	77.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010405.t1	G7I6H5	89.5	124	12	1	1	124	1	123	6.7e-53	216.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene010405.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010405.t1	MTR_1g023820	89.516	124	12	1	1	124	1	123	1.03e-76	223
MS.gene010405.t1	MTR_5g036670	58.824	153	8	2	17	114	73	225	1.33e-46	160
MS.gene010405.t1	MTR_8g062700	40.909	154	35	3	17	114	72	225	1.67e-23	94.7
MS.gene010405.t1	MTR_8g062700	40.909	154	35	3	17	114	72	225	1.76e-23	94.7
MS.gene010405.t1	MTR_6g008970	35.669	157	42	5	17	114	93	249	1.49e-18	80.5
MS.gene010405.t1	MTR_6g008995	64.000	50	17	1	17	66	67	115	2.00e-14	68.6
MS.gene010405.t1	MTR_6g008995	64.000	50	17	1	17	66	10	58	2.07e-14	68.6
MS.gene010405.t1	MTR_1g105960	54.167	48	22	0	17	64	31	78	6.11e-14	63.5
MS.gene010405.t1	MTR_5g055670	68.293	41	13	0	20	60	89	129	1.13e-12	60.8
MS.gene010405.t1	MTR_1g105955	60.526	38	15	0	17	54	46	83	1.04e-11	59.3
MS.gene010405.t1	MTR_3g006065	60.870	46	18	0	11	56	115	160	2.20e-11	58.5
MS.gene010405.t1	MTR_2g097830	35.000	80	52	0	2	81	82	161	2.21e-11	58.2
MS.gene010405.t1	MTR_3g006150	60.870	46	18	0	11	56	115	160	5.62e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene010405.t1	AT3G01320	33.766	154	46	2	17	114	87	240	2.88e-18	79.7
MS.gene010405.t1	AT3G01320	33.766	154	46	2	17	114	87	240	2.99e-18	79.7
MS.gene010405.t1	AT1G59890	33.333	153	47	2	17	114	75	227	1.05e-17	78.2
MS.gene010405.t1	AT1G59890	33.333	153	47	2	17	114	75	227	1.06e-17	78.2
MS.gene010405.t1	AT1G59890	33.333	153	47	2	17	114	75	227	1.11e-17	78.2
MS.gene010405.t1	AT1G59890	33.333	153	47	2	17	114	75	227	1.12e-17	78.2
MS.gene010405.t1	AT1G70060	36.129	155	41	5	17	114	44	197	4.06e-17	76.3
MS.gene010405.t1	AT1G70060	36.129	155	41	5	17	114	44	197	4.06e-17	76.3
MS.gene010405.t1	AT1G24190	42.202	109	22	1	17	84	44	152	5.13e-17	76.3
MS.gene010405.t1	AT1G24190	42.202	109	22	1	17	84	44	152	5.28e-17	76.3
MS.gene010405.t1	AT1G24190	42.202	109	22	1	17	84	44	152	5.38e-17	76.3
MS.gene010405.t1	AT5G15020	35.714	154	42	5	17	114	82	234	8.34e-17	75.5
MS.gene010405.t1	AT5G15020	35.714	154	42	5	17	114	82	234	8.59e-17	75.5
MS.gene010405.t1	AT1G10450	62.500	48	18	0	17	64	114	161	2.03e-15	71.6
MS.gene010405.t1	AT1G10450	62.500	48	18	0	17	64	63	110	2.13e-15	71.6
MS.gene010405.t1	AT1G10450	62.500	48	18	0	17	64	63	110	2.13e-15	71.6
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	6.97e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	6.97e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	6.98e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	7.18e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	7.31e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	7.38e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	7.38e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	7.39e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	7.39e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	274	316	7.60e-11	58.5
MS.gene010405.t1	AT4G12020	58.140	43	18	0	21	63	341	383	8.08e-11	58.5

Find 27 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCATGGCCCTTTGGCAAAA+AGG	0.248205	5.4:-11031679	None:intergenic
ATGCAGCATCCGGGTGGTTT+TGG	0.280374	5.4:-11031892	None:intergenic
ACTTCGATCTCTTTCTCGTT+TGG	0.311785	5.4:-11031372	None:intergenic
GCATGAGTAGATGCAGCATC+CGG	0.446564	5.4:-11031902	None:intergenic
AGGGTAATCTCATGGCCCTT+TGG	0.451315	5.4:-11031687	None:intergenic
CACATACCTCTTTGTAGACC+AGG	0.459268	5.4:-11031749	None:intergenic
TAGACCAGGGTGTTAGACTT+TGG	0.465686	5.4:-11031735	None:intergenic
GGTCATAGAGACTTGATCTT+CGG	0.474274	5.4:+11031648	MS.gene010405:CDS
ACGGCGAGTGCATGTTGAAA+AGG	0.478594	5.4:+11031976	MS.gene010405:CDS
AGGGTGTTAGACTTTGGTTG+AGG	0.550511	5.4:-11031729	None:intergenic
TTGCTCATCCTCAGACGAAA+GGG	0.552798	5.4:-11031706	None:intergenic
TGAGATTACCCTTTCGTCTG+AGG	0.555293	5.4:+11031698	MS.gene010405:CDS
GCATGCCTTTGACTACAGAA+AGG	0.565598	5.4:-11032020	None:intergenic
CATGCCTTTGACTACAGAAA+GGG	0.567694	5.4:-11032019	None:intergenic
ACATGCACTCGCCGTAGTGT+TGG	0.571378	5.4:-11031968	None:intergenic
CATGAGTAGATGCAGCATCC+GGG	0.581255	5.4:-11031901	None:intergenic
CAGACGAAAGGGTAATCTCA+TGG	0.592774	5.4:-11031695	None:intergenic
GTTGCTCATCCTCAGACGAA+AGG	0.601293	5.4:-11031707	None:intergenic
TGCACTCTACCAAAACCACC+CGG	0.633017	5.4:+11031883	MS.gene010405:CDS
ATTTGTAGAGTTGATACTGA+TGG	0.633224	5.4:+11031594	MS.gene010405:intron
AGAGCTCCTATCACAAAACA+TGG	0.657685	5.4:-11031939	None:intergenic
TAACACCCTGGTCTACAAAG+AGG	0.665433	5.4:+11031743	MS.gene010405:CDS
GCTCTGCAATGCCAACACTA+CGG	0.674753	5.4:+11031957	MS.gene010405:CDS
TCAACCAAAGTCTAACACCC+TGG	0.678062	5.4:+11031731	MS.gene010405:CDS
ACATACCTCTTTGTAGACCA+GGG	0.680303	5.4:-11031748	None:intergenic
TGGTGTCATTGAAAGAGTGA+AGG	0.685356	5.4:+11031614	MS.gene010405:CDS
GAGTAGATGCAGCATCCGGG+TGG	0.688313	5.4:-11031898	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTGATTTGTTTATCCTTT+TGG	+	chr5.4:11031568-11031587	MS.gene010405:intron	20.0%
!!!	CCATTTTTTGAGAATTCTAT+GGG	+	chr5.4:11031441-11031460	MS.gene010405:intron	25.0%
!!!	TCCATTTTTTGAGAATTCTA+TGG	+	chr5.4:11031440-11031459	MS.gene010405:intron	25.0%
	CCCATAGAATTCTCAAAAAA+TGG	-	chr5.4:11031444-11031463	None:intergenic	30.0%
	GTCAGGTAAATTTGTAATGT+TGG	+	chr5.4:11031780-11031799	MS.gene010405:intron	30.0%
!	AGAATTCCATGTTTTGTGAT+AGG	+	chr5.4:11031933-11031952	MS.gene010405:CDS	30.0%
!	ATTTAACACCTTTTTGCCAA+AGG	+	chr5.4:11031671-11031690	MS.gene010405:CDS	30.0%
!	TTGAGAATTCTATGGGATTT+TGG	+	chr5.4:11031448-11031467	MS.gene010405:intron	30.0%
!	TTTAACACCTTTTTGCCAAA+GGG	+	chr5.4:11031672-11031691	MS.gene010405:CDS	30.0%
!!	ATTTGTAGAGTTGATACTGA+TGG	+	chr5.4:11031594-11031613	MS.gene010405:intron	30.0%
!!	CTTACTGTTTTTCTTTTCGA+CGG	-	chr5.4:11031400-11031419	None:intergenic	30.0%
	AACTCTACAAATGCACCAAA+AGG	-	chr5.4:11031586-11031605	None:intergenic	35.0%
	AATCATAATTGCACTTCTCC+AGG	+	chr5.4:11031856-11031875	MS.gene010405:intron	35.0%
	CGTCGAAAAGAAAAACAGTA+AGG	+	chr5.4:11031398-11031417	MS.gene010405:CDS	35.0%
!	AGAGTGAAGGAACTTTTTAG+AGG	+	chr5.4:11031627-11031646	MS.gene010405:CDS	35.0%
!	AGGTATGTGTTGTTATAGTC+AGG	+	chr5.4:11031763-11031782	MS.gene010405:intron	35.0%
	ACATACCTCTTTGTAGACCA+GGG	-	chr5.4:11031751-11031770	None:intergenic	40.0%
	ACTTCGATCTCTTTCTCGTT+TGG	-	chr5.4:11031375-11031394	None:intergenic	40.0%
	AGAGCTCCTATCACAAAACA+TGG	-	chr5.4:11031942-11031961	None:intergenic	40.0%
	CTCCAGTACTTGACATATTG+CGG	+	chr5.4:11031820-11031839	MS.gene010405:intron	40.0%
	GGTCATAGAGACTTGATCTT+CGG	+	chr5.4:11031648-11031667	MS.gene010405:CDS	40.0%
!!	GAATTCTATGGGATTTTGGC+TGG	+	chr5.4:11031452-11031471	MS.gene010405:intron	40.0%
!!	TGGTGTCATTGAAAGAGTGA+AGG	+	chr5.4:11031614-11031633	MS.gene010405:CDS	40.0%
	CACATACCTCTTTGTAGACC+AGG	-	chr5.4:11031752-11031771	None:intergenic	45.0%
	CACCGCAATATGTCAAGTAC+TGG	-	chr5.4:11031825-11031844	None:intergenic	45.0%
	CAGACGAAAGGGTAATCTCA+TGG	-	chr5.4:11031698-11031717	None:intergenic	45.0%
	TAACACCCTGGTCTACAAAG+AGG	+	chr5.4:11031743-11031762	MS.gene010405:CDS	45.0%
	TCAACCAAAGTCTAACACCC+TGG	+	chr5.4:11031731-11031750	MS.gene010405:CDS	45.0%
	TGAGATTACCCTTTCGTCTG+AGG	+	chr5.4:11031698-11031717	MS.gene010405:CDS	45.0%
	TTGCTCATCCTCAGACGAAA+GGG	-	chr5.4:11031709-11031728	None:intergenic	45.0%
!	AGGGTGTTAGACTTTGGTTG+AGG	-	chr5.4:11031732-11031751	None:intergenic	45.0%
!	TAGACCAGGGTGTTAGACTT+TGG	-	chr5.4:11031738-11031757	None:intergenic	45.0%
!!	CGCAATATGTCAAGTACTGG+AGG	-	chr5.4:11031822-11031841	None:intergenic	45.0%
!!!	ATGGGATTTTGGCTGGAACT+TGG	+	chr5.4:11031459-11031478	MS.gene010405:intron	45.0%
	ACGGCGAGTGCATGTTGAAA+AGG	+	chr5.4:11031976-11031995	MS.gene010405:CDS	50.0%
	AGGGTAATCTCATGGCCCTT+TGG	-	chr5.4:11031690-11031709	None:intergenic	50.0%
	CATGAGTAGATGCAGCATCC+GGG	-	chr5.4:11031904-11031923	None:intergenic	50.0%
	CTCATGGCCCTTTGGCAAAA+AGG	-	chr5.4:11031682-11031701	None:intergenic	50.0%
	GCATGAGTAGATGCAGCATC+CGG	-	chr5.4:11031905-11031924	None:intergenic	50.0%
	GCTCTGCAATGCCAACACTA+CGG	+	chr5.4:11031957-11031976	MS.gene010405:CDS	50.0%
	GTTGCTCATCCTCAGACGAA+AGG	-	chr5.4:11031710-11031729	None:intergenic	50.0%
	TGCACTCTACCAAAACCACC+CGG	+	chr5.4:11031883-11031902	MS.gene010405:CDS	50.0%
	TTTGGTAGAGTGCAGCAACC+TGG	-	chr5.4:11031877-11031896	None:intergenic	50.0%
	ACATGCACTCGCCGTAGTGT+TGG	-	chr5.4:11031971-11031990	None:intergenic	55.0%
!	ATGCAGCATCCGGGTGGTTT+TGG	-	chr5.4:11031895-11031914	None:intergenic	55.0%
	GAGTAGATGCAGCATCCGGG+TGG	-	chr5.4:11031901-11031920	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	11031361	11032030	11031361	ID=MS.gene010405
chr5.4	mRNA	11031361	11032030	11031361	ID=MS.gene010405.t1;Parent=MS.gene010405
chr5.4	exon	11031361	11031419	11031361	ID=MS.gene010405.t1.exon1;Parent=MS.gene010405.t1
chr5.4	CDS	11031361	11031419	11031361	ID=cds.MS.gene010405.t1;Parent=MS.gene010405.t1
chr5.4	exon	11031602	11031764	11031602	ID=MS.gene010405.t1.exon2;Parent=MS.gene010405.t1
chr5.4	CDS	11031602	11031764	11031602	ID=cds.MS.gene010405.t1;Parent=MS.gene010405.t1
chr5.4	exon	11031878	11032030	11031878	ID=MS.gene010405.t1.exon3;Parent=MS.gene010405.t1
chr5.4	CDS	11031878	11032030	11031878	ID=cds.MS.gene010405.t1;Parent=MS.gene010405.t1

Gene Sequence

>MS.gene010405

ATGTGCGTGATCCAAACGAGAAAGAGATCGAAGTTGCCGTCGAAAAGAAAAACAGTAAGGTATATTTTGCTGACGATTGTCCATTTTTTGAGAATTCTATGGGATTTTGGCTGGAACTTGGATCACAATTATTTTTTCAAATCGACACATTTTTCTGTTTGAAGTTAGAAATATGCATGTGAGAGAGATTATGAATTTGTTAGTTGTAATTGATTTGTTTATCCTTTTGGTGCATTTGTAGAGTTGATACTGATGGTGTCATTGAAAGAGTGAAGGAACTTTTTAGAGGTCATAGAGACTTGATCTTCGGATTTAACACCTTTTTGCCAAAGGGCCATGAGATTACCCTTTCGTCTGAGGATGAGCAACCTCAACCAAAGTCTAACACCCTGGTCTACAAAGAGGTATGTGTTGTTATAGTCAGGTAAATTTGTAATGTTGGTTTTTTTTTTTTATTTCCTCCAGTACTTGACATATTGCGGTGATACACATTTTAATCATAATTGCACTTCTCCAGGTTGCTGCACTCTACCAAAACCACCCGGATGCTGCATCTACTCATGCTTTTGCTCAGAATTCCATGTTTTGTGATAGGAGCTCTGCAATGCCAACACTACGGCGAGTGCATGTTGAAAAGGTTTGTCTTCAGTTCTTATGTCCCTTTCTGTAG

Protein sequence

>MS.gene010405.t1

MCVIQTRKRSKLPSKRKTVRVDTDGVIERVKELFRGHRDLIFGFNTFLPKGHEITLSSEDEQPQPKSNTLVYKEVAALYQNHPDAASTHAFAQNSMFCDRSSAMPTLRRVHVEKVCLQFLCPFL