AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010443

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010443.t1 MTR_5g019030 81.501 373 14 2 1 318 262 634 0.0 601
MS.gene010443.t1 MTR_5g019040 94.515 237 13 0 318 554 185 421 3.07e-151 447
MS.gene010443.t1 MTR_8g078300 52.033 246 99 6 320 554 180 417 6.01e-65 223
MS.gene010443.t1 MTR_5g042440 33.840 263 136 7 320 553 195 448 1.05e-27 118
MS.gene010443.t1 MTR_5g019050 42.969 128 70 2 427 554 335 459 6.50e-25 109
MS.gene010443.t1 MTR_1g021845 32.584 267 130 13 321 554 197 446 1.17e-23 105
MS.gene010443.t1 MTR_3g080170 32.735 223 120 9 355 554 245 460 1.19e-21 99.4
MS.gene010443.t1 MTR_5g085790 39.865 148 86 2 408 553 315 461 5.17e-21 97.4
MS.gene010443.t1 MTR_8g070050 29.577 142 100 0 174 315 81 222 1.65e-18 85.1
MS.gene010443.t1 MTR_7g029650 26.198 313 168 14 303 553 154 465 4.83e-16 82.0
MS.gene010443.t1 MTR_5g033490 38.462 130 68 4 433 554 293 418 3.13e-15 79.3
MS.gene010443.t1 MTR_3g037490 30.822 146 97 2 147 292 101 242 3.83e-14 75.9
MS.gene010443.t1 MTR_3g037490 29.851 134 94 0 176 309 196 329 1.28e-13 73.9
MS.gene010443.t1 MTR_3g008280 32.680 153 84 5 412 553 410 554 5.57e-14 75.5
MS.gene010443.t1 MTR_3g008280 32.680 153 84 5 412 553 418 562 5.66e-14 75.5
MS.gene010443.t1 MTR_2g080080 32.456 228 117 9 347 553 380 591 1.18e-13 74.3
MS.gene010443.t1 MTR_0014s0250 27.830 212 133 5 95 306 315 506 1.94e-13 73.6
MS.gene010443.t1 MTR_6g081920 31.148 122 84 0 176 297 279 400 2.37e-13 72.8
MS.gene010443.t1 MTR_4g133920 36.923 130 70 5 435 554 21 148 3.45e-13 71.2
MS.gene010443.t1 MTR_3g080050 36.429 140 71 6 423 554 298 427 4.33e-13 72.4
MS.gene010443.t1 MTR_7g012890 29.379 177 120 3 139 315 344 515 4.65e-13 72.0
MS.gene010443.t1 MTR_6g079590 31.967 122 83 0 176 297 380 501 7.62e-13 71.6
MS.gene010443.t1 MTR_8g074920 30.049 203 108 9 369 553 3 189 8.29e-13 70.9
MS.gene010443.t1 MTR_6g077800 26.562 128 94 0 173 300 227 354 8.50e-13 71.2
MS.gene010443.t1 MTR_5g029690 29.915 117 82 0 176 292 254 370 9.19e-13 71.6
MS.gene010443.t1 MTR_1g112240 28.889 135 96 0 176 310 245 379 9.69e-13 71.2
MS.gene010443.t1 MTR_1g112240 23.913 184 136 2 109 292 217 396 8.09e-12 68.2
MS.gene010443.t1 MTR_2g070020 34.483 174 90 7 398 553 754 921 1.08e-12 71.6
MS.gene010443.t1 MTR_8g070060 28.261 138 87 1 174 311 522 647 1.11e-12 71.2
MS.gene010443.t1 MTR_4g081655 36.923 130 64 6 436 553 527 650 1.70e-12 70.9
MS.gene010443.t1 MTR_4g081655 36.923 130 64 6 436 553 527 650 1.71e-12 70.5
MS.gene010443.t1 MTR_6g069200 29.008 131 93 0 176 306 33 163 1.89e-12 67.0
MS.gene010443.t1 MTR_8g057660 31.852 135 83 3 425 551 36 169 2.03e-12 68.9
MS.gene010443.t1 MTR_4g081655 36.923 130 64 6 436 553 492 615 2.03e-12 70.5
MS.gene010443.t1 MTR_4g108270 38.060 134 55 5 442 553 78 205 2.67e-12 69.3
MS.gene010443.t1 MTR_6g069840 29.032 186 128 2 112 297 299 480 2.67e-12 69.7
MS.gene010443.t1 MTR_0014s0180 28.889 180 121 3 121 300 331 503 2.69e-12 69.7
MS.gene010443.t1 MTR_6g069300 30.000 140 97 1 176 315 382 520 2.80e-12 69.7
MS.gene010443.t1 MTR_4g091670 32.547 212 104 10 373 553 440 643 2.88e-12 70.1
MS.gene010443.t1 MTR_2g011180 29.412 221 117 8 362 553 431 641 3.30e-12 69.7
MS.gene010443.t1 MTR_1g068960 27.811 169 113 3 140 303 453 617 3.51e-12 69.7
MS.gene010443.t1 MTR_1g068960 24.365 197 129 2 109 297 247 431 3.12e-11 66.6
MS.gene010443.t1 MTR_3g105900 24.739 287 163 8 10 293 364 600 3.53e-12 70.1
MS.gene010443.t1 MTR_3g105900 25.243 206 148 2 105 309 241 441 3.69e-12 70.1
MS.gene010443.t1 MTR_3g105900 26.531 196 137 3 108 300 837 1028 8.83e-11 65.5
MS.gene010443.t1 MTR_2g011180 29.412 221 117 8 362 553 431 641 3.63e-12 69.7
MS.gene010443.t1 MTR_6g069840 29.032 186 128 2 112 297 315 496 3.78e-12 69.3
MS.gene010443.t1 MTR_4g091670 32.547 212 104 10 373 553 440 643 4.06e-12 69.7
MS.gene010443.t1 MTR_0090s0020 29.474 190 107 9 379 553 431 608 4.53e-12 69.3
MS.gene010443.t1 MTR_0090s0020 29.474 190 107 9 379 553 431 608 4.76e-12 69.3
MS.gene010443.t1 MTR_8g054410 28.481 158 109 2 140 297 358 511 5.11e-12 68.9
MS.gene010443.t1 MTR_0090s0020 29.474 190 107 9 379 553 431 608 5.32e-12 68.9
MS.gene010443.t1 MTR_0090s0020 29.474 190 107 9 379 553 431 608 5.37e-12 68.9
MS.gene010443.t1 MTR_5g013070 29.353 201 106 6 366 553 445 622 5.38e-12 69.3
MS.gene010443.t1 MTR_8g107050 27.230 213 120 5 112 293 317 525 5.85e-12 68.9
MS.gene010443.t1 MTR_1g086870 30.702 228 126 12 345 553 185 399 6.08e-12 68.9
MS.gene010443.t1 MTR_8g107050 27.230 213 120 5 112 293 317 525 6.27e-12 68.9
MS.gene010443.t1 MTR_2g104790 30.693 202 104 9 378 554 43 233 6.98e-12 67.8
MS.gene010443.t1 MTR_3g034990 29.167 168 114 2 148 315 362 524 7.43e-12 68.6
MS.gene010443.t1 MTR_3g448620 26.471 204 140 5 113 315 315 509 8.05e-12 68.2
MS.gene010443.t1 MTR_2g022810 35.185 162 81 7 410 554 38 192 8.66e-12 67.4
MS.gene010443.t1 MTR_6g072030 30.508 118 82 0 175 292 262 379 1.05e-11 68.2
MS.gene010443.t1 MTR_6g072030 31.624 117 80 0 176 292 473 589 1.96e-11 67.0
MS.gene010443.t1 MTR_6g079920 27.011 174 123 2 144 314 368 540 1.07e-11 67.8
MS.gene010443.t1 MTR_6g079920 26.667 135 99 0 176 310 263 397 3.89e-11 66.2
MS.gene010443.t1 MTR_1g050495 27.941 136 98 0 176 311 236 371 1.41e-11 67.4
MS.gene010443.t1 MTR_8g101320 33.594 128 68 5 437 553 429 550 1.53e-11 67.8
MS.gene010443.t1 MTR_8g101300 33.594 128 68 5 437 553 457 578 1.64e-11 67.8
MS.gene010443.t1 MTR_6g079890 26.667 135 99 0 176 310 216 350 1.64e-11 67.0
MS.gene010443.t1 MTR_6g478210 28.689 122 87 0 176 297 80 201 1.80e-11 66.2
MS.gene010443.t1 MTR_8g461110 30.952 210 112 8 357 553 609 798 1.85e-11 67.8
MS.gene010443.t1 MTR_8g067735 31.250 208 104 10 367 553 280 469 1.93e-11 67.4
MS.gene010443.t1 MTR_1g048080 27.933 179 123 4 121 298 570 743 1.94e-11 67.4
MS.gene010443.t1 MTR_6g080200 31.200 125 86 0 176 300 358 482 1.95e-11 67.0
MS.gene010443.t1 MTR_6g036780 33.168 202 104 11 370 554 611 798 2.15e-11 67.4
MS.gene010443.t1 MTR_4g107180 28.244 131 94 0 176 306 271 401 2.15e-11 66.6
MS.gene010443.t1 MTR_6g069170 24.747 198 132 4 109 291 130 325 2.19e-11 65.9
MS.gene010443.t1 MTR_6g093030 34.375 128 65 5 437 553 461 580 2.25e-11 67.0
MS.gene010443.t1 MTR_3g061020 25.668 187 135 1 120 306 179 361 2.27e-11 66.2
MS.gene010443.t1 MTR_0019s0210 29.231 195 130 4 121 315 333 519 2.37e-11 66.6
MS.gene010443.t1 MTR_2g073250 34.356 163 83 8 402 553 446 595 2.41e-11 67.0
MS.gene010443.t1 MTR_6g079830 26.667 135 99 0 176 310 263 397 2.97e-11 66.6
MS.gene010443.t1 MTR_6g079830 25.424 177 128 2 141 314 365 540 8.14e-11 65.1
MS.gene010443.t1 MTR_3g415610 30.055 183 101 7 392 553 157 333 2.99e-11 66.2
MS.gene010443.t1 MTR_5g037410 30.939 181 105 7 389 554 267 442 3.43e-11 66.2
MS.gene010443.t1 MTR_1g055295 28.462 130 92 1 169 297 864 993 3.52e-11 66.6
MS.gene010443.t1 MTR_6g478130 26.738 187 132 3 129 315 191 372 3.53e-11 65.9
MS.gene010443.t1 MTR_0014s0260 27.119 118 86 0 176 293 207 324 3.88e-11 65.9
MS.gene010443.t1 MTR_0110s0060 26.257 179 128 1 128 306 329 503 3.96e-11 65.9
MS.gene010443.t1 MTR_4g014900 34.959 123 62 5 444 554 158 274 4.07e-11 65.1
MS.gene010443.t1 MTR_6g078190 21.941 237 153 3 109 314 187 422 4.21e-11 65.9
MS.gene010443.t1 MTR_5g085700 26.778 239 142 9 330 553 635 855 4.31e-11 66.2
MS.gene010443.t1 MTR_4g014900 34.959 123 62 5 444 554 158 274 4.51e-11 65.5
MS.gene010443.t1 MTR_4g069970 37.984 129 63 6 437 553 128 251 4.61e-11 65.5
MS.gene010443.t1 MTR_6g478220 27.632 152 98 3 148 291 24 171 4.70e-11 62.8
MS.gene010443.t1 MTR_7g118240 26.829 164 105 3 140 297 117 271 4.79e-11 65.5
MS.gene010443.t1 MTR_3g109100 32.500 160 86 7 408 553 420 571 4.86e-11 66.2
MS.gene010443.t1 MTR_4g069970 37.984 129 63 6 437 553 128 251 4.95e-11 65.5
MS.gene010443.t1 MTR_4g014900 34.959 123 62 5 444 554 158 274 5.37e-11 65.1
MS.gene010443.t1 MTR_1g015050 35.156 128 64 6 437 553 406 525 5.37e-11 65.9
MS.gene010443.t1 MTR_4g069970 37.984 129 63 6 437 553 128 251 5.66e-11 64.7
MS.gene010443.t1 MTR_2g096850 27.329 161 95 6 407 553 270 422 5.84e-11 65.9
MS.gene010443.t1 MTR_1g013040 34.959 123 62 5 444 554 153 269 5.96e-11 65.1
MS.gene010443.t1 MTR_1g013040 34.959 123 62 5 444 554 153 269 5.97e-11 65.1
MS.gene010443.t1 MTR_0573s0020 26.738 187 132 3 129 315 56 237 6.68e-11 63.5
MS.gene010443.t1 MTR_6g445300 29.323 133 86 2 173 297 311 443 7.08e-11 65.1
MS.gene010443.t1 MTR_6g036870 35.359 181 85 12 369 531 611 777 7.39e-11 65.5
MS.gene010443.t1 MTR_3g086120 28.800 250 143 12 330 554 480 719 7.80e-11 65.5
MS.gene010443.t1 MTR_3g086120 28.800 250 143 12 330 554 480 719 7.96e-11 65.5
MS.gene010443.t1 MTR_8g070910 29.146 199 116 7 372 553 195 385 9.54e-11 65.1
MS.gene010443.t1 MTR_6g079440 27.481 131 95 0 176 306 372 502 9.79e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010443.t1 AT5G66631 50.802 374 127 3 2 318 263 636 8.39e-122 374
MS.gene010443.t1 AT2G33580 41.085 129 68 3 426 553 349 470 2.66e-20 95.5
MS.gene010443.t1 AT2G23770 29.889 271 140 9 321 553 194 452 1.59e-17 86.7
MS.gene010443.t1 AT3G18110 26.066 211 153 1 105 315 373 580 6.81e-15 79.0
MS.gene010443.t1 AT3G18110 26.486 185 131 3 117 300 280 460 1.56e-11 68.2
MS.gene010443.t1 AT2G31400 24.915 293 169 6 2 292 320 563 9.26e-15 78.2
MS.gene010443.t1 AT5G11020 30.472 233 127 10 346 554 31 252 1.13e-14 76.6
MS.gene010443.t1 AT2G31400 24.915 293 169 6 2 292 320 563 1.44e-14 77.4
MS.gene010443.t1 AT1G69730 34.021 194 102 9 378 553 373 558 3.78e-13 72.8
MS.gene010443.t1 AT5G39710 27.811 169 118 2 109 277 285 449 3.85e-13 72.8
MS.gene010443.t1 AT2G32630 24.378 201 142 2 109 306 234 427 1.18e-12 71.2
MS.gene010443.t1 AT1G62930 28.085 235 147 5 109 337 230 448 1.57e-12 70.9
MS.gene010443.t1 AT1G62930 25.954 131 97 0 176 306 433 563 5.99e-11 65.9
MS.gene010443.t1 AT5G11020 32.487 197 97 10 384 554 5 191 2.06e-12 69.3
MS.gene010443.t1 AT2G06000 31.783 129 85 2 174 300 172 299 2.35e-12 70.1
MS.gene010443.t1 AT2G06000 31.783 129 85 2 174 300 172 299 2.35e-12 70.1
MS.gene010443.t1 AT4G20140 37.795 127 65 4 442 554 944 1070 2.53e-12 70.5
MS.gene010443.t1 AT4G31850 27.273 209 138 4 95 303 404 598 3.80e-12 70.1
MS.gene010443.t1 AT3G53380 29.319 191 104 6 376 553 313 485 4.11e-12 69.7
MS.gene010443.t1 AT1G74850 28.037 214 145 4 99 309 211 418 4.31e-12 69.7
MS.gene010443.t1 AT2G07020 37.037 135 64 7 437 560 407 531 5.13e-12 69.3
MS.gene010443.t1 AT1G61390 28.788 198 114 8 373 553 275 462 6.26e-12 68.9
MS.gene010443.t1 AT5G61990 31.818 110 75 0 176 285 630 739 6.92e-12 68.9
MS.gene010443.t1 AT1G18390 28.889 225 118 9 361 554 244 457 7.26e-12 68.6
MS.gene010443.t1 AT3G22470 28.042 189 132 2 105 293 216 400 7.31e-12 68.6
MS.gene010443.t1 AT3G22470 28.042 189 132 2 105 293 216 400 8.16e-12 68.6
MS.gene010443.t1 AT3G22470 27.869 122 88 0 176 297 318 439 4.89e-11 65.9
MS.gene010443.t1 AT5G42440 27.778 198 113 9 377 553 4 192 8.25e-12 67.4
MS.gene010443.t1 AT1G62670 28.936 235 151 5 109 340 231 452 9.58e-12 68.2
MS.gene010443.t1 AT1G62670 28.936 235 151 5 109 340 231 452 9.58e-12 68.2
MS.gene010443.t1 AT1G61390 28.788 198 114 8 373 553 443 630 9.63e-12 68.6
MS.gene010443.t1 AT1G62590 28.108 185 129 2 109 293 235 415 1.14e-11 68.2
MS.gene010443.t1 AT1G62590 28.108 185 129 2 109 293 235 415 1.14e-11 68.2
MS.gene010443.t1 AT1G12620 26.064 188 131 4 105 290 218 399 1.53e-11 67.4
MS.gene010443.t1 AT3G59730 25.185 270 150 10 322 553 194 449 1.53e-11 67.4
MS.gene010443.t1 AT1G63130 27.826 230 144 5 109 332 231 444 1.59e-11 67.4
MS.gene010443.t1 AT1G63130 27.826 230 144 5 109 332 231 444 1.59e-11 67.4
MS.gene010443.t1 AT1G30290 25.758 198 141 3 105 300 353 546 1.71e-11 67.8
MS.gene010443.t1 AT1G30290 25.758 198 141 3 105 300 353 546 1.98e-11 67.4
MS.gene010443.t1 AT5G02860 23.958 192 139 2 118 309 263 447 2.01e-11 67.4
MS.gene010443.t1 AT5G02860 23.958 192 139 2 118 309 263 447 2.01e-11 67.4
MS.gene010443.t1 AT5G02860 23.958 192 139 2 118 309 263 447 2.01e-11 67.4
MS.gene010443.t1 AT5G02860 23.958 192 139 2 118 309 263 447 2.01e-11 67.4
MS.gene010443.t1 AT5G02860 23.958 192 139 2 118 309 263 447 2.01e-11 67.4
MS.gene010443.t1 AT1G12775 26.178 191 133 4 105 293 234 418 2.22e-11 67.0
MS.gene010443.t1 AT2G24370 33.125 160 85 7 408 553 436 587 2.31e-11 67.0
MS.gene010443.t1 AT1G12700 27.692 130 94 0 173 302 349 478 2.68e-11 66.6
MS.gene010443.t1 AT1G12700 27.692 130 94 0 173 302 349 478 2.68e-11 66.6
MS.gene010443.t1 AT1G12700 27.692 130 94 0 173 302 333 462 2.75e-11 66.6
MS.gene010443.t1 AT1G62910 28.261 230 143 5 109 332 233 446 3.63e-11 66.2
MS.gene010443.t1 AT1G61380 28.351 194 109 9 373 553 275 451 3.73e-11 66.2
MS.gene010443.t1 AT3G22670 25.984 127 94 0 176 302 276 402 3.92e-11 66.2
MS.gene010443.t1 AT3G54980 26.389 216 152 4 79 292 528 738 4.20e-11 66.2
MS.gene010443.t1 AT3G54980 26.389 216 152 4 79 292 528 738 4.20e-11 66.2
MS.gene010443.t1 AT1G79490 29.221 154 101 2 135 286 314 461 5.73e-11 65.9
MS.gene010443.t1 AT1G61380 28.351 194 109 9 373 553 422 598 5.88e-11 65.9
MS.gene010443.t1 AT2G16250 25.581 215 138 7 361 553 428 642 5.96e-11 65.9
MS.gene010443.t1 AT5G61400 29.861 144 94 1 150 293 393 529 6.53e-11 65.5
MS.gene010443.t1 AT1G09820 23.913 184 136 2 109 292 271 450 7.13e-11 65.5
MS.gene010443.t1 AT1G65790 31.034 203 101 9 381 553 436 629 7.45e-11 65.5
MS.gene010443.t1 AT5G59700 28.095 210 117 8 368 554 394 592 7.86e-11 65.5
MS.gene010443.t1 AT1G09900 25.405 185 131 3 109 293 147 324 7.90e-11 65.1
MS.gene010443.t1 AT1G18900 22.667 225 167 2 89 310 351 571 8.13e-11 65.5
MS.gene010443.t1 AT1G18900 22.667 225 167 2 89 310 351 571 8.13e-11 65.5
MS.gene010443.t1 AT1G18900 22.667 225 167 2 89 310 351 571 8.13e-11 65.5
MS.gene010443.t1 AT1G65800 30.693 202 103 10 381 553 440 633 8.21e-11 65.5
MS.gene010443.t1 AT1G18900 22.667 225 167 2 89 310 351 571 8.51e-11 65.5
MS.gene010443.t1 AT3G53700 23.188 207 155 1 109 315 445 647 8.71e-11 65.5
MS.gene010443.t1 AT5G35380 36.134 119 57 6 448 555 413 523 8.95e-11 65.1
MS.gene010443.t1 AT1G62680 26.316 171 117 1 176 346 333 494 9.34e-11 65.1
MS.gene010443.t1 AT1G62680 26.316 171 117 1 176 346 333 494 9.34e-11 65.1
MS.gene010443.t1 AT1G63400 24.405 168 117 4 136 300 402 562 9.90e-11 65.1

Find 111 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATCTGGCTCTTATAAT+TGG 0.262266 5.4:+11544708 MS.gene010443:CDS
TTTGAAGATATGAAGAATTC+TGG 0.266472 5.4:+11542898 MS.gene010443:CDS
AAGTTACTTTCAGGTGTTTC+AGG 0.271026 5.4:+11544846 MS.gene010443:CDS
GTCTATGAGTATGCTGAAAA+TGG 0.278284 5.4:+11545110 MS.gene010443:CDS
ACTGCAGATAAGTTACTTTC+AGG 0.304363 5.4:+11544837 MS.gene010443:CDS
TTGGCTGGATCCGCGGTCTT+TGG 0.315039 5.4:+11542208 MS.gene010443:CDS
GAAGATGGTTGATCAAGTTT+TGG 0.326422 5.4:-11544663 None:intergenic
ACCCTTGTTAGTTCAAAGTT+TGG 0.344284 5.4:+11544552 MS.gene010443:CDS
GTTAATGAGATGCAAGTTGA+TGG 0.346251 5.4:+11541963 MS.gene010443:CDS
GCATTAGACCTGAAAATGTT+TGG 0.360031 5.4:-11542726 None:intergenic
AAAGTCAATGATTAACCTTA+TGG 0.364393 5.4:-11542509 None:intergenic
TGGCTAGTGCGTTACAGAAT+TGG 0.365279 5.4:+11542228 MS.gene010443:CDS
ATGTCTCTGTGGATTATTCT+TGG 0.369404 5.4:-11545259 None:intergenic
AACTCTTTGATGGCCGAATC+AGG 0.382129 5.4:-11542928 None:intergenic
TGGAAATATTGTGAAACTAA+TGG 0.382814 5.4:+11545052 MS.gene010443:CDS
AGTAAAATGTTTGATGAAGT+TGG 0.386223 5.4:+11541933 None:intergenic
CAAGCCAACAATGTATGAAA+TGG 0.386975 5.4:+11544878 MS.gene010443:CDS
CTTCACTAGCAACTCTTTGA+TGG 0.403178 5.4:-11542938 None:intergenic
GAAGGATGAAGGATTTCTTA+AGG 0.405813 5.4:+11542028 MS.gene010443:CDS
TTCTGGTTCCATACCTGATT+CGG 0.410204 5.4:+11542915 MS.gene010443:CDS
GTCCTTCCACTTGCATTACC+TGG 0.422582 5.4:+11543030 MS.gene010443:CDS
GATGAGTCTATCAACTAATT+CGG 0.423510 5.4:-11542446 None:intergenic
TACGTCCATGTCGTGCTAGA+AGG 0.426527 5.4:-11542423 None:intergenic
GCCAGACGCGTATCTGTATA+AGG 0.430893 5.4:+11542822 MS.gene010443:CDS
AACAATGATCGAATACTTTG+TGG 0.433378 5.4:+11542574 MS.gene010443:CDS
CAACAAGAGCTGCACTGGCT+TGG 0.436660 5.4:+11542869 MS.gene010443:CDS
GGAAATATTGTGAAACTAAT+GGG 0.440270 5.4:+11545053 MS.gene010443:CDS
ACTTGAAGAGTGGTTGTTCT+CGG 0.440441 5.4:+11545136 MS.gene010443:CDS
ATCGGAATCCCTTCCACACT+TGG 0.440957 5.4:-11542653 None:intergenic
ACACTCTTGATTCTTTCATC+CGG 0.443612 5.4:-11542080 None:intergenic
ACATCCATTTCATACATTGT+TGG 0.446716 5.4:-11544882 None:intergenic
GATTCACTTAAAACCTTGGC+TGG 0.457260 5.4:+11542193 MS.gene010443:CDS
AAACAGTTTCCATCATTGTC+AGG 0.459516 5.4:-11545083 None:intergenic
TTGCAGCAGGTGAAGGATGA+AGG 0.460732 5.4:+11542017 MS.gene010443:CDS
CTTGATCAACCATCTTCAAA+TGG 0.466617 5.4:+11544669 MS.gene010443:CDS
CACTCTTGATTCTTTCATCC+GGG 0.466810 5.4:-11542079 None:intergenic
GGTGAGTTTGTTATTGCAGC+AGG 0.472558 5.4:+11542004 MS.gene010443:CDS
ACTCGGTGGTTTCGCTTACA+TGG 0.474294 5.4:+11545177 MS.gene010443:CDS
AGTTGCTAGTGAAGAGCCTC+TGG 0.476859 5.4:+11542947 MS.gene010443:CDS
TCATCTCATCAAGCATGTCC+AGG 0.481198 5.4:-11542678 None:intergenic
GTTTACAAGGGTAATATAGA+TGG 0.483555 5.4:+11544957 MS.gene010443:CDS
GTGAAGAGCCTCTGGAAAGA+AGG 0.484880 5.4:+11542955 MS.gene010443:CDS
GATTGGTGAATCAGTTTACA+AGG 0.486742 5.4:+11544944 MS.gene010443:CDS
CATCTCGAAGATCAGAATAG+AGG 0.487430 5.4:+11542465 MS.gene010443:CDS
TGAAGGATTTCTTAAGGATG+TGG 0.487856 5.4:+11542034 MS.gene010443:CDS
TAAGTCAACAAGAGCTGCAC+TGG 0.490541 5.4:+11542864 MS.gene010443:CDS
ATAGCCATGGATGTTGCAAT+TGG 0.492998 5.4:+11545215 MS.gene010443:CDS
CTTATAATTGGTATTAGCCT+AGG 0.502273 5.4:+11544720 MS.gene010443:CDS
CAAAGTTCGTGTGGACAACT+AGG 0.503289 5.4:+11542282 MS.gene010443:CDS
CTGAGTGAGAATTGTAAGAT+TGG 0.506708 5.4:+11544927 MS.gene010443:CDS
ATCAAGCATGTCCAGGGTAT+CGG 0.509612 5.4:-11542671 None:intergenic
ACAATGATCGAATACTTTGT+GGG 0.511017 5.4:+11542575 MS.gene010443:CDS
CACCAAACTTTGAACTAACA+AGG 0.512914 5.4:-11544554 None:intergenic
ATGGGATTCACTTAAAACCT+TGG 0.520730 5.4:+11542189 MS.gene010443:CDS
GAACATTAACCAAGTGTGGA+AGG 0.523805 5.4:+11542644 MS.gene010443:CDS
ACCTTATACAGATACGCGTC+TGG 0.524198 5.4:-11542823 None:intergenic
CAGACCAATTGCAACATCCA+TGG 0.524233 5.4:-11545219 None:intergenic
GTATGAAATGGATGTGATCA+TGG 0.524330 5.4:+11544890 MS.gene010443:CDS
TTTGATGGCCGAATCAGGTA+TGG 0.524348 5.4:-11542923 None:intergenic
ATGTGGATGAATTGTTTGAA+GGG 0.525014 5.4:+11542051 MS.gene010443:CDS
AAATGCTGATGCTGCTCTGA+AGG 0.525513 5.4:+11542546 MS.gene010443:CDS
GATGTGGATGAATTGTTTGA+AGG 0.528226 5.4:+11542050 MS.gene010443:CDS
TCTAGTGCTGATACCTCATC+AGG 0.535975 5.4:-11542254 None:intergenic
ACTTACAATAACCTTCTGTC+AGG 0.537808 5.4:-11542846 None:intergenic
TTAGTTTCACAATATTTCCA+TGG 0.537931 5.4:-11545049 None:intergenic
ACGTCCATGTCGTGCTAGAA+GGG 0.540180 5.4:-11542422 None:intergenic
ATTAACCTTATGGTACTGAA+TGG 0.545713 5.4:-11542499 None:intergenic
GGTTAATGTTCTTAAAAGAG+AGG 0.546099 5.4:-11542632 None:intergenic
AACATTAACCAAGTGTGGAA+GGG 0.552157 5.4:+11542645 MS.gene010443:CDS
TTAAGAACATTAACCAAGTG+TGG 0.552345 5.4:+11542640 MS.gene010443:CDS
ATCTTTGAAAACTTCAAACT+CGG 0.553009 5.4:+11545160 MS.gene010443:CDS
GACTACCATTCAGTACCATA+AGG 0.558337 5.4:+11542494 MS.gene010443:CDS
GCCTCTGGAAAGAAGGTAGA+CGG 0.559458 5.4:+11542962 MS.gene010443:CDS
AATTGCAACATCCATGGCTA+TGG 0.562845 5.4:-11545213 None:intergenic
ATCTCGAAGATCAGAATAGA+GGG 0.563251 5.4:+11542466 MS.gene010443:CDS
GAAAGAAGGTAGACGGAGAG+AGG 0.569327 5.4:+11542969 MS.gene010443:CDS
TCCGTCTACCTTCTTTCCAG+AGG 0.577819 5.4:-11542963 None:intergenic
CTGTATAAGGTCCTGACAGA+AGG 0.578969 5.4:+11542835 MS.gene010443:CDS
TGATGAGGTATCAGCACTAG+AGG 0.580742 5.4:+11542256 MS.gene010443:CDS
TCAGAGAATAACCATAGCCA+TGG 0.584198 5.4:+11545202 MS.gene010443:CDS
TTTGTTATTGCAGCAGGTGA+AGG 0.584701 5.4:+11542010 MS.gene010443:CDS
GTTCGTGTGGACAACTAGGT+TGG 0.586491 5.4:+11542286 MS.gene010443:CDS
AAGTAACTTATCTGCAGTCT+CGG 0.588582 5.4:-11544832 None:intergenic
TAAAACCTTGGCTGGATCCG+CGG 0.590765 5.4:+11542201 MS.gene010443:CDS
CTGACAGTCCATACATGACC+AGG 0.594467 5.4:-11543048 None:intergenic
TGGAAGGGATTCCGATACCC+TGG 0.598845 5.4:+11542660 MS.gene010443:CDS
CATTGAAAAGAGTTTCTGCA+CGG 0.599322 5.4:-11542779 None:intergenic
CAATATACGTACACAAGTGA+TGG 0.600647 5.4:-11544771 None:intergenic
TTTGAAAACTTCAAACTCGG+TGG 0.605841 5.4:+11545163 MS.gene010443:CDS
AAAGACCGCGGATCCAGCCA+AGG 0.608186 5.4:-11542206 None:intergenic
ATTGTTTGAAGGGATTTGCC+CGG 0.613868 5.4:+11542061 MS.gene010443:CDS
AGGGATCAAAACTGAACAGT+CGG 0.614320 5.4:-11544628 None:intergenic
AACAAACTCACCGTTCGACT+AGG 0.617703 5.4:-11541993 None:intergenic
CATCTCATCAAGCATGTCCA+GGG 0.618669 5.4:-11542677 None:intergenic
ACATGACCAGGTAATGCAAG+TGG 0.620837 5.4:-11543036 None:intergenic
GCAGCTCTCTTCAACAGCTG+CGG 0.624107 5.4:-11542992 None:intergenic
TGGAATATTACCTAGTCGAA+CGG 0.624584 5.4:+11541983 MS.gene010443:CDS
GGTGTGTCTCCTGACAATGA+TGG 0.628519 5.4:+11545074 MS.gene010443:CDS
TAGAGGGTGCAAAGTTCGTG+TGG 0.631089 5.4:+11542273 MS.gene010443:CDS
ATGACCCTTCTAGCACGACA+TGG 0.636441 5.4:+11542418 MS.gene010443:CDS
ATTGGTGAATCAGTTTACAA+GGG 0.638333 5.4:+11544945 MS.gene010443:CDS
GACCAGGTAATGCAAGTGGA+AGG 0.640755 5.4:-11543032 None:intergenic
ACAGAATTGGAGTCCTGATG+AGG 0.642414 5.4:+11542241 MS.gene010443:CDS
GAGCTGCGAGGAAGTAAATG+TGG 0.649634 5.4:+11543008 MS.gene010443:CDS
GATGAGGTATCAGCACTAGA+GGG 0.650518 5.4:+11542257 MS.gene010443:CDS
ACCAAACTTTGAACTAACAA+GGG 0.655493 5.4:-11544553 None:intergenic
GAAATGGATGTGATCATGGA+AGG 0.661178 5.4:+11544894 MS.gene010443:CDS
ATTCTGCAGAAGGTAAACCA+TGG 0.662360 5.4:+11545032 MS.gene010443:CDS
AAAATGGATCACTTGAAGAG+TGG 0.692384 5.4:+11545126 MS.gene010443:CDS
AGCTGTTGAAGAGAGCTGCG+AGG 0.717575 5.4:+11542996 MS.gene010443:CDS
AACGCACTAGCCAAAGACCG+CGG 0.753982 5.4:-11542218 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAATTGCATATATATAA+TGG + chr5.4:11544167-11544186 MS.gene010443:intron 10.0%
!! ATGCAATTATAATATAATAC+AGG - chr5.4:11544159-11544178 None:intergenic 15.0%
!! TAAAGATGGTAAGAATAATA+TGG - chr5.4:11544333-11544352 None:intergenic 20.0%
!!! AATTTTCTTACAAAAAGAGT+TGG + chr5.4:11543525-11543544 MS.gene010443:intron 20.0%
!!! CTTAAACTGTGATTTATTTT+GGG + chr5.4:11543225-11543244 MS.gene010443:intron 20.0%
!!! CTTTTTGTAAGAAAATTAGA+AGG - chr5.4:11543522-11543541 None:intergenic 20.0%
!!! TCAAATAGTTTAGTTTCAAA+GGG + chr5.4:11543105-11543124 MS.gene010443:intron 20.0%
!!! TTCAAATAGTTTAGTTTCAA+AGG + chr5.4:11543104-11543123 MS.gene010443:intron 20.0%
!!! TTTTTTTTGTTGGTGTAAAA+GGG + chr5.4:11543675-11543694 MS.gene010443:intron 20.0%
!!! TTTTTTTTTGTTGGTGTAAA+AGG + chr5.4:11543674-11543693 MS.gene010443:intron 20.0%
! AAAGTCAATGATTAACCTTA+TGG - chr5.4:11542512-11542531 None:intergenic 25.0%
! AGTATCTTATTACTTATGTG+TGG + chr5.4:11544511-11544530 MS.gene010443:intron 25.0%
! ATTCTGCCATAAGAATATAA+CGG - chr5.4:11543787-11543806 None:intergenic 25.0%
! CCAAAATAAATCACAGTTTA+AGG - chr5.4:11543227-11543246 None:intergenic 25.0%
! GATATATTATGAGTAAATCC+AGG - chr5.4:11542383-11542402 None:intergenic 25.0%
! GGAAATATTGTGAAACTAAT+GGG + chr5.4:11545053-11545072 MS.gene010443:CDS 25.0%
! TATATTGAAACCTATGTAAC+TGG + chr5.4:11544105-11544124 MS.gene010443:intron 25.0%
! TATGGTATTATAACCCAATT+TGG - chr5.4:11544315-11544334 None:intergenic 25.0%
! TATGGTTTAACGTTTAACAA+AGG - chr5.4:11544199-11544218 None:intergenic 25.0%
! TATTATTTGTTCCCTATTTC+TGG - chr5.4:11544056-11544075 None:intergenic 25.0%
! TCAAAGAATCAATTGAACAA+AGG + chr5.4:11544483-11544502 MS.gene010443:intron 25.0%
! TGGAAATATTGTGAAACTAA+TGG + chr5.4:11545052-11545071 MS.gene010443:CDS 25.0%
! TTAACACCGTTATATTCTTA+TGG + chr5.4:11543778-11543797 MS.gene010443:intron 25.0%
! TTACACCAACAAAAAAAAAG+AGG - chr5.4:11543673-11543692 None:intergenic 25.0%
! TTAGTTTCACAATATTTCCA+TGG - chr5.4:11545052-11545071 None:intergenic 25.0%
! TTGCACAATCATATAATGAA+TGG + chr5.4:11543401-11543420 MS.gene010443:intron 25.0%
! TTTCTTACAAAAAGAAGAGT+TGG + chr5.4:11543564-11543583 MS.gene010443:intron 25.0%
! TTTGAAGATATGAAGAATTC+TGG + chr5.4:11542898-11542917 MS.gene010443:CDS 25.0%
!! ATCTTTGAAAACTTCAAACT+CGG + chr5.4:11545160-11545179 MS.gene010443:CDS 25.0%
!! CCTTAAACTGTGATTTATTT+TGG + chr5.4:11543224-11543243 MS.gene010443:intron 25.0%
!! CTTGATGAGATGATTTTAAA+TGG + chr5.4:11542688-11542707 MS.gene010443:CDS 25.0%
!! TGTTCAATTGATTCTTTGAA+GGG - chr5.4:11544482-11544501 None:intergenic 25.0%
!! TTGTTCAATTGATTCTTTGA+AGG - chr5.4:11544483-11544502 None:intergenic 25.0%
!!! AGGTTAATCATTGACTTTTA+TGG + chr5.4:11542514-11542533 MS.gene010443:CDS 25.0%
!!! GAGTTTTGAGAATGTATTAT+TGG + chr5.4:11543286-11543305 MS.gene010443:intron 25.0%
!!! GGTTAATCATTGACTTTTAT+GGG + chr5.4:11542515-11542534 MS.gene010443:CDS 25.0%
AAATCACAGTTTAAGGAACT+TGG - chr5.4:11543220-11543239 None:intergenic 30.0%
AACAATGATCGAATACTTTG+TGG + chr5.4:11542574-11542593 MS.gene010443:CDS 30.0%
AATCAAAAAAGATGCTTCTG+AGG + chr5.4:11545001-11545020 MS.gene010443:CDS 30.0%
ACAATGATCGAATACTTTGT+GGG + chr5.4:11542575-11542594 MS.gene010443:CDS 30.0%
ACATCCATTTCATACATTGT+TGG - chr5.4:11544885-11544904 None:intergenic 30.0%
ACCAAACTTTGAACTAACAA+GGG - chr5.4:11544556-11544575 None:intergenic 30.0%
ATGCCTTTACCAAAATGTTA+AGG - chr5.4:11544239-11544258 None:intergenic 30.0%
ATGTGGATGAATTGTTTGAA+GGG + chr5.4:11542051-11542070 MS.gene010443:CDS 30.0%
ATTGGTGAATCAGTTTACAA+GGG + chr5.4:11544945-11544964 MS.gene010443:CDS 30.0%
GAATATTAATCCCTCGAATA+GGG + chr5.4:11543717-11543736 MS.gene010443:intron 30.0%
GATGAGTCTATCAACTAATT+CGG - chr5.4:11542449-11542468 None:intergenic 30.0%
GCATGATTTCATACTTGTTT+GGG + chr5.4:11544364-11544383 MS.gene010443:intron 30.0%
GGTTAATGTTCTTAAAAGAG+AGG - chr5.4:11542635-11542654 None:intergenic 30.0%
GTTTACAAGGGTAATATAGA+TGG + chr5.4:11544957-11544976 MS.gene010443:CDS 30.0%
TATTAGATGTCAAGCTAATG+TGG + chr5.4:11543993-11544012 MS.gene010443:intron 30.0%
TCATATAATGAATGGACCTT+TGG + chr5.4:11543409-11543428 MS.gene010443:intron 30.0%
TGAATATTAATCCCTCGAAT+AGG + chr5.4:11543716-11543735 MS.gene010443:intron 30.0%
TGCCTTTACCAAAATGTTAA+GGG - chr5.4:11544238-11544257 None:intergenic 30.0%
TGCGCAGAATATAATAAAGA+TGG - chr5.4:11544347-11544366 None:intergenic 30.0%
TTAAGAACATTAACCAAGTG+TGG + chr5.4:11542640-11542659 MS.gene010443:CDS 30.0%
TTTCAAATCTTAGACACTGT+TGG - chr5.4:11544395-11544414 None:intergenic 30.0%
TTTGAGAATGTATTATTGGC+AGG + chr5.4:11543290-11543309 MS.gene010443:intron 30.0%
! ACAGATATCCAAACATTTTC+AGG + chr5.4:11542718-11542737 MS.gene010443:CDS 30.0%
! ATGCAGTATTTTTCACAACT+TGG + chr5.4:11542745-11542764 MS.gene010443:CDS 30.0%
! ATTAACCTTATGGTACTGAA+TGG - chr5.4:11542502-11542521 None:intergenic 30.0%
! CTTATAATTGGTATTAGCCT+AGG + chr5.4:11544720-11544739 MS.gene010443:CDS 30.0%
! CTTTTTCTTCCATTTGAAGA+TGG - chr5.4:11544681-11544700 None:intergenic 30.0%
! TACTGCACCTTAAAACTTTT+TGG - chr5.4:11543259-11543278 None:intergenic 30.0%
! TTTTCAGCTAACATTTCAAC+TGG - chr5.4:11544585-11544604 None:intergenic 30.0%
!! AAATGAAAAAAGCACTTCCT+AGG - chr5.4:11544740-11544759 None:intergenic 30.0%
!! CAAAATCTGGCTCTTATAAT+TGG + chr5.4:11544708-11544727 MS.gene010443:CDS 30.0%
!!! AAACTGTGATTTATTTTGGG+AGG + chr5.4:11543228-11543247 MS.gene010443:intron 30.0%
!!! AACTGTGATTTATTTTGGGA+GGG + chr5.4:11543229-11543248 MS.gene010443:intron 30.0%
!!! ACTTTTTTTTAGGCATAGCA+TGG - chr5.4:11543852-11543871 None:intergenic 30.0%
!!! CTTTTTTTTAGGCATAGCAT+GGG - chr5.4:11543851-11543870 None:intergenic 30.0%
!!! GAGTTCCTCTTTTTTTTTGT+TGG + chr5.4:11543665-11543684 MS.gene010443:intron 30.0%
AAAAAGCAGCAGTCAAAATC+TGG + chr5.4:11544695-11544714 MS.gene010443:CDS 35.0%
AAAAAGCTAGACTCAAGCAA+AGG + chr5.4:11543934-11543953 MS.gene010443:intron 35.0%
AAAATGGATCACTTGAAGAG+TGG + chr5.4:11545126-11545145 MS.gene010443:CDS 35.0%
AAACAGTTTCCATCATTGTC+AGG - chr5.4:11545086-11545105 None:intergenic 35.0%
AACATTAACCAAGTGTGGAA+GGG + chr5.4:11542645-11542664 MS.gene010443:CDS 35.0%
AACGATTGAATGAGATGACA+TGG - chr5.4:11543878-11543897 None:intergenic 35.0%
AAGTAACTTATCTGCAGTCT+CGG - chr5.4:11544835-11544854 None:intergenic 35.0%
ACACTCTTGATTCTTTCATC+CGG - chr5.4:11542083-11542102 None:intergenic 35.0%
ACCCTTGTTAGTTCAAAGTT+TGG + chr5.4:11544552-11544571 MS.gene010443:CDS 35.0%
ACGATTGAATGAGATGACAT+GGG - chr5.4:11543877-11543896 None:intergenic 35.0%
ACTTACAATAACCTTCTGTC+AGG - chr5.4:11542849-11542868 None:intergenic 35.0%
AGTAGCTATGTACCAGAAAT+AGG + chr5.4:11544041-11544060 MS.gene010443:intron 35.0%
ATCTCGAAGATCAGAATAGA+GGG + chr5.4:11542466-11542485 MS.gene010443:CDS 35.0%
ATGGGATTCACTTAAAACCT+TGG + chr5.4:11542189-11542208 MS.gene010443:CDS 35.0%
CAAGCCAACAATGTATGAAA+TGG + chr5.4:11544878-11544897 MS.gene010443:CDS 35.0%
CAATATACGTACACAAGTGA+TGG - chr5.4:11544774-11544793 None:intergenic 35.0%
CACAATGTGATCATGTAGAT+GGG + chr5.4:11542171-11542190 MS.gene010443:intron 35.0%
CACCAAACTTTGAACTAACA+AGG - chr5.4:11544557-11544576 None:intergenic 35.0%
CATCAAGTTAGATGGTCATT+AGG + chr5.4:11544256-11544275 MS.gene010443:intron 35.0%
CATTGAAAAGAGTTTCTGCA+CGG - chr5.4:11542782-11542801 None:intergenic 35.0%
CGCATGATTTCATACTTGTT+TGG + chr5.4:11544363-11544382 MS.gene010443:intron 35.0%
CTGAGTGAGAATTGTAAGAT+TGG + chr5.4:11544927-11544946 MS.gene010443:CDS 35.0%
CTTGATCAACCATCTTCAAA+TGG + chr5.4:11544669-11544688 MS.gene010443:CDS 35.0%
GAAGGATGAAGGATTTCTTA+AGG + chr5.4:11542028-11542047 MS.gene010443:CDS 35.0%
GATCCTTCATTGTATTCACT+TGG + chr5.4:11543358-11543377 MS.gene010443:intron 35.0%
GATGTGGATGAATTGTTTGA+AGG + chr5.4:11542050-11542069 MS.gene010443:CDS 35.0%
GATTGGTGAATCAGTTTACA+AGG + chr5.4:11544944-11544963 MS.gene010443:CDS 35.0%
GCAAGATACTTAGAAGTTTC+AGG + chr5.4:11542312-11542331 MS.gene010443:intron 35.0%
GCATTAGACCTGAAAATGTT+TGG - chr5.4:11542729-11542748 None:intergenic 35.0%
GCCTTTACCAAAATGTTAAG+GGG - chr5.4:11544237-11544256 None:intergenic 35.0%
GTAGCTATGTACCAGAAATA+GGG + chr5.4:11544042-11544061 MS.gene010443:intron 35.0%
GTATGAAATGGATGTGATCA+TGG + chr5.4:11544890-11544909 MS.gene010443:CDS 35.0%
GTCTATGAGTATGCTGAAAA+TGG + chr5.4:11545110-11545129 MS.gene010443:CDS 35.0%
TCACAATGTGATCATGTAGA+TGG + chr5.4:11542170-11542189 MS.gene010443:intron 35.0%
TCATCTCATTCAATCGTTCT+TGG + chr5.4:11543880-11543899 MS.gene010443:intron 35.0%
TCTAAGCACAAAGTAACAAG+CGG + chr5.4:11543184-11543203 MS.gene010443:intron 35.0%
TCTTATTACTTATGTGTGGC+AGG + chr5.4:11544515-11544534 MS.gene010443:intron 35.0%
TGAAGGATTTCTTAAGGATG+TGG + chr5.4:11542034-11542053 MS.gene010443:CDS 35.0%
TGACCAAGTGAATACAATGA+AGG - chr5.4:11543364-11543383 None:intergenic 35.0%
TGGAATATTACCTAGTCGAA+CGG + chr5.4:11541983-11542002 MS.gene010443:CDS 35.0%
TTGTGCTTAGATTTCATGCA+TGG - chr5.4:11543175-11543194 None:intergenic 35.0%
TTTGAAAACTTCAAACTCGG+TGG + chr5.4:11545163-11545182 MS.gene010443:CDS 35.0%
! AAGTTACTTTCAGGTGTTTC+AGG + chr5.4:11544846-11544865 MS.gene010443:CDS 35.0%
! ACTGCAGATAAGTTACTTTC+AGG + chr5.4:11544837-11544856 MS.gene010443:CDS 35.0%
! GAAGATGGTTGATCAAGTTT+TGG - chr5.4:11544666-11544685 None:intergenic 35.0%
! GTTAATGAGATGCAAGTTGA+TGG + chr5.4:11541963-11541982 MS.gene010443:CDS 35.0%
! TTCTATTGATAGCAGTGATG+AGG + chr5.4:11542109-11542128 MS.gene010443:intron 35.0%
! TTGTTTGCCCCTTAACATTT+TGG + chr5.4:11544227-11544246 MS.gene010443:intron 35.0%
! TTTCTGGTACATAGCTACTT+CGG - chr5.4:11544040-11544059 None:intergenic 35.0%
!! AGTTTTGGTAAACTTGTCAC+AGG - chr5.4:11544651-11544670 None:intergenic 35.0%
!! CAATGATGGAAACTGTTTTC+TGG + chr5.4:11545088-11545107 MS.gene010443:CDS 35.0%
!! GTTTTGGTAAACTTGTCACA+GGG - chr5.4:11544650-11544669 None:intergenic 35.0%
!! TTACTTCTAGCTTAACCAGT+GGG - chr5.4:11543821-11543840 None:intergenic 35.0%
!!! AATCGTTCTTGGCTTGTTTT+CGG + chr5.4:11543891-11543910 MS.gene010443:intron 35.0%
!!! ACTGTGATTTATTTTGGGAG+GGG + chr5.4:11543230-11543249 MS.gene010443:intron 35.0%
!!! GACATGGGTCACTTTTTTTT+AGG - chr5.4:11543862-11543881 None:intergenic 35.0%
AAGAAATTCCACGCAGTATC+AGG - chr5.4:11542341-11542360 None:intergenic 40.0%
AATTGCAACATCCATGGCTA+TGG - chr5.4:11545216-11545235 None:intergenic 40.0%
ACCTCTATCATATAGCCCAA+TGG - chr5.4:11543144-11543163 None:intergenic 40.0%
ACTTGAAGAGTGGTTGTTCT+CGG + chr5.4:11545136-11545155 MS.gene010443:CDS 40.0%
AGGGATCAAAACTGAACAGT+CGG - chr5.4:11544631-11544650 None:intergenic 40.0%
ATAGCCATGGATGTTGCAAT+TGG + chr5.4:11545215-11545234 MS.gene010443:CDS 40.0%
ATAGCCCAATGGATAACTCT+AGG - chr5.4:11543133-11543152 None:intergenic 40.0%
ATCGTGTATGGACACCAAAT+TGG + chr5.4:11544298-11544317 MS.gene010443:intron 40.0%
ATGTTATCCAACCCTATTCG+AGG - chr5.4:11543731-11543750 None:intergenic 40.0%
ATTAATCCCTCGAATAGGGT+TGG + chr5.4:11543721-11543740 MS.gene010443:intron 40.0%
ATTCTGCAGAAGGTAAACCA+TGG + chr5.4:11545032-11545051 MS.gene010443:CDS 40.0%
ATTGTTTGAAGGGATTTGCC+CGG + chr5.4:11542061-11542080 MS.gene010443:CDS 40.0%
CAAAGGAGAGTAGGTAAGAA+GGG + chr5.4:11543951-11543970 MS.gene010443:intron 40.0%
CACTCTTGATTCTTTCATCC+GGG - chr5.4:11542082-11542101 None:intergenic 40.0%
CATCTCGAAGATCAGAATAG+AGG + chr5.4:11542465-11542484 MS.gene010443:CDS 40.0%
CTTCACTAGCAACTCTTTGA+TGG - chr5.4:11542941-11542960 None:intergenic 40.0%
GAAATGGATGTGATCATGGA+AGG + chr5.4:11544894-11544913 MS.gene010443:CDS 40.0%
GAACATTAACCAAGTGTGGA+AGG + chr5.4:11542644-11542663 MS.gene010443:CDS 40.0%
GACTACCATTCAGTACCATA+AGG + chr5.4:11542494-11542513 MS.gene010443:CDS 40.0%
GATTCACTTAAAACCTTGGC+TGG + chr5.4:11542193-11542212 MS.gene010443:CDS 40.0%
GGAGCTAAAAATTCTGCAGA+AGG + chr5.4:11545022-11545041 MS.gene010443:CDS 40.0%
GGGCAAACAAGCAAAAGATA+TGG - chr5.4:11544217-11544236 None:intergenic 40.0%
GTTGACACTTCCAGTTACAT+AGG - chr5.4:11544118-11544137 None:intergenic 40.0%
TCAGAGAATAACCATAGCCA+TGG + chr5.4:11545202-11545221 MS.gene010443:CDS 40.0%
TCCATTGGGCTATATGATAG+AGG + chr5.4:11543140-11543159 MS.gene010443:intron 40.0%
TCGTGTATGGACACCAAATT+GGG + chr5.4:11544299-11544318 MS.gene010443:intron 40.0%
TGTTATCCAACCCTATTCGA+GGG - chr5.4:11543730-11543749 None:intergenic 40.0%
TTGCATTACCTGGTCATGTA+TGG + chr5.4:11543040-11543059 MS.gene010443:intron 40.0%
! CGTGCAGAAACTCTTTTCAA+TGG + chr5.4:11542780-11542799 MS.gene010443:CDS 40.0%
! GGAATTAGCTCTTGTTCTGA+TGG + chr5.4:11543330-11543349 MS.gene010443:intron 40.0%
! GGGGTTGCCAAAAAGTTTTA+AGG + chr5.4:11543249-11543268 MS.gene010443:intron 40.0%
! TTTTCAATGGTCAGACAGAG+CGG + chr5.4:11542793-11542812 MS.gene010443:CDS 40.0%
!! CTTACTTCTAGCTTAACCAG+TGG - chr5.4:11543822-11543841 None:intergenic 40.0%
!! GCCCCTTAACATTTTGGTAA+AGG + chr5.4:11544233-11544252 MS.gene010443:intron 40.0%
!! GGTAAAGGCATCAAGTTAGA+TGG + chr5.4:11544248-11544267 MS.gene010443:intron 40.0%
!! TTCTGGTTCCATACCTGATT+CGG + chr5.4:11542915-11542934 MS.gene010443:CDS 40.0%
!! TTTGTTATTGCAGCAGGTGA+AGG + chr5.4:11542010-11542029 MS.gene010443:CDS 40.0%
AAATGCTGATGCTGCTCTGA+AGG + chr5.4:11542546-11542565 MS.gene010443:CDS 45.0%
AACAAACTCACCGTTCGACT+AGG - chr5.4:11541996-11542015 None:intergenic 45.0%
ACAGAATTGGAGTCCTGATG+AGG + chr5.4:11542241-11542260 MS.gene010443:CDS 45.0%
ACATGACCAGGTAATGCAAG+TGG - chr5.4:11543039-11543058 None:intergenic 45.0%
ACCTTATACAGATACGCGTC+TGG - chr5.4:11542826-11542845 None:intergenic 45.0%
AGACTCAAGCAAAGGAGAGT+AGG + chr5.4:11543942-11543961 MS.gene010443:intron 45.0%
ATCAAGCATGTCCAGGGTAT+CGG - chr5.4:11542674-11542693 None:intergenic 45.0%
CAAAGTTCGTGTGGACAACT+AGG + chr5.4:11542282-11542301 MS.gene010443:CDS 45.0%
CAGACCAATTGCAACATCCA+TGG - chr5.4:11545222-11545241 None:intergenic 45.0%
CAGTGATGAGGATGAGAATG+AGG + chr5.4:11542121-11542140 MS.gene010443:intron 45.0%
CATCTCATCAAGCATGTCCA+GGG - chr5.4:11542680-11542699 None:intergenic 45.0%
CTGTATAAGGTCCTGACAGA+AGG + chr5.4:11542835-11542854 MS.gene010443:CDS 45.0%
GCAAAGGAGAGTAGGTAAGA+AGG + chr5.4:11543950-11543969 MS.gene010443:intron 45.0%
GCTCTCAACACATCAACCAA+AGG - chr5.4:11543428-11543447 None:intergenic 45.0%
GGTGCCTAGAGTTATCCATT+GGG + chr5.4:11543126-11543145 MS.gene010443:intron 45.0%
TAAGTCAACAAGAGCTGCAC+TGG + chr5.4:11542864-11542883 MS.gene010443:CDS 45.0%
TCATCTCATCAAGCATGTCC+AGG - chr5.4:11542681-11542700 None:intergenic 45.0%
TGCGTGGAATTTCTTCTGCT+GGG + chr5.4:11542346-11542365 MS.gene010443:intron 45.0%
TGGCTAGTGCGTTACAGAAT+TGG + chr5.4:11542228-11542247 MS.gene010443:CDS 45.0%
! AACTCTTTGATGGCCGAATC+AGG - chr5.4:11542931-11542950 None:intergenic 45.0%
! GATGAGGTATCAGCACTAGA+GGG + chr5.4:11542257-11542276 MS.gene010443:CDS 45.0%
! TGATGAGGTATCAGCACTAG+AGG + chr5.4:11542256-11542275 MS.gene010443:CDS 45.0%
! TTTGATGGCCGAATCAGGTA+TGG - chr5.4:11542926-11542945 None:intergenic 45.0%
!! GGTGAGTTTGTTATTGCAGC+AGG + chr5.4:11542004-11542023 MS.gene010443:CDS 45.0%
!! TCTAGTGCTGATACCTCATC+AGG - chr5.4:11542257-11542276 None:intergenic 45.0%
!! TGCTGACTTCTGCAACTTGA+GGG + chr5.4:11543462-11543481 MS.gene010443:intron 45.0%
ACGTCCATGTCGTGCTAGAA+GGG - chr5.4:11542425-11542444 None:intergenic 50.0%
AGTTGCTAGTGAAGAGCCTC+TGG + chr5.4:11542947-11542966 MS.gene010443:CDS 50.0%
ATCGGAATCCCTTCCACACT+TGG - chr5.4:11542656-11542675 None:intergenic 50.0%
ATGACCCTTCTAGCACGACA+TGG + chr5.4:11542418-11542437 MS.gene010443:CDS 50.0%
CAAGTTGCAGAAGTCAGCAG+AGG - chr5.4:11543461-11543480 None:intergenic 50.0%
CAGCTAGCTGACATCGTGTA+TGG + chr5.4:11544286-11544305 MS.gene010443:intron 50.0%
CATACACGATGTCAGCTAGC+TGG - chr5.4:11544288-11544307 None:intergenic 50.0%
CTGACAGTCCATACATGACC+AGG - chr5.4:11543051-11543070 None:intergenic 50.0%
CTGCGTGGAATTTCTTCTGC+TGG + chr5.4:11542345-11542364 MS.gene010443:intron 50.0%
GAAAGAAGGTAGACGGAGAG+AGG + chr5.4:11542969-11542988 MS.gene010443:CDS 50.0%
GAATTCGAACTCGAACCCAC+TGG + chr5.4:11543803-11543822 MS.gene010443:intron 50.0%
GACCAGGTAATGCAAGTGGA+AGG - chr5.4:11543035-11543054 None:intergenic 50.0%
GAGCTGCGAGGAAGTAAATG+TGG + chr5.4:11543008-11543027 MS.gene010443:CDS 50.0%
GAGGATGAGAATGAGGACGA+GGG + chr5.4:11542128-11542147 MS.gene010443:intron 50.0%
GCCAGACGCGTATCTGTATA+AGG + chr5.4:11542822-11542841 MS.gene010443:CDS 50.0%
GGGTGCCTAGAGTTATCCAT+TGG + chr5.4:11543125-11543144 MS.gene010443:intron 50.0%
GGTGTGTCTCCTGACAATGA+TGG + chr5.4:11545074-11545093 MS.gene010443:CDS 50.0%
GTCCTTCCACTTGCATTACC+TGG + chr5.4:11543030-11543049 MS.gene010443:CDS 50.0%
GTTCGTGTGGACAACTAGGT+TGG + chr5.4:11542286-11542305 MS.gene010443:CDS 50.0%
TAAAACCTTGGCTGGATCCG+CGG + chr5.4:11542201-11542220 MS.gene010443:CDS 50.0%
TACGTCCATGTCGTGCTAGA+AGG - chr5.4:11542426-11542445 None:intergenic 50.0%
TCCGTCTACCTTCTTTCCAG+AGG - chr5.4:11542966-11542985 None:intergenic 50.0%
TGAGGATGAGAATGAGGACG+AGG + chr5.4:11542127-11542146 MS.gene010443:intron 50.0%
TTGCAGCAGGTGAAGGATGA+AGG + chr5.4:11542017-11542036 MS.gene010443:CDS 50.0%
! ACTCGGTGGTTTCGCTTACA+TGG + chr5.4:11545177-11545196 MS.gene010443:CDS 50.0%
! GCCTCTGGAAAGAAGGTAGA+CGG + chr5.4:11542962-11542981 MS.gene010443:CDS 50.0%
! GCTGACTTCTGCAACTTGAG+GGG + chr5.4:11543463-11543482 MS.gene010443:intron 50.0%
! GTGAAGAGCCTCTGGAAAGA+AGG + chr5.4:11542955-11542974 MS.gene010443:CDS 50.0%
! TAGAGGGTGCAAAGTTCGTG+TGG + chr5.4:11542273-11542292 MS.gene010443:CDS 50.0%
!! CAGGACATGAGTTGATGCTC+AGG + chr5.4:11543309-11543328 MS.gene010443:intron 50.0%
!! CTGCTGACTTCTGCAACTTG+AGG + chr5.4:11543461-11543480 MS.gene010443:intron 50.0%
AACGCACTAGCCAAAGACCG+CGG - chr5.4:11542221-11542240 None:intergenic 55.0%
AGCTGTTGAAGAGAGCTGCG+AGG + chr5.4:11542996-11543015 MS.gene010443:CDS 55.0%
GATGAGAATGAGGACGAGGG+TGG + chr5.4:11542131-11542150 MS.gene010443:intron 55.0%
GCAGCTCTCTTCAACAGCTG+CGG - chr5.4:11542995-11543014 None:intergenic 55.0%
TCAGGTCACCTGATACTGCG+TGG + chr5.4:11542330-11542349 MS.gene010443:intron 55.0%
TGGAAGGGATTCCGATACCC+TGG + chr5.4:11542660-11542679 MS.gene010443:CDS 55.0%
!! CAACAAGAGCTGCACTGGCT+TGG + chr5.4:11542869-11542888 MS.gene010443:CDS 55.0%
AAAGACCGCGGATCCAGCCA+AGG - chr5.4:11542209-11542228 None:intergenic 60.0%
CACGATGTCAGCTAGCTGGC+TGG - chr5.4:11544284-11544303 None:intergenic 60.0%
TGCTGGGTTGCTGATCGACC+TGG + chr5.4:11542362-11542381 MS.gene010443:intron 60.0%
TTGGCTGGATCCGCGGTCTT+TGG + chr5.4:11542208-11542227 MS.gene010443:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 11541939 11545268 11541939 ID=MS.gene010443
chr5.4 mRNA 11541939 11545268 11541939 ID=MS.gene010443.t1;Parent=MS.gene010443
chr5.4 exon 11541939 11542104 11541939 ID=MS.gene010443.t1.exon1;Parent=MS.gene010443.t1
chr5.4 CDS 11541939 11542104 11541939 ID=cds.MS.gene010443.t1;Parent=MS.gene010443.t1
chr5.4 exon 11542189 11542326 11542189 ID=MS.gene010443.t1.exon2;Parent=MS.gene010443.t1
chr5.4 CDS 11542189 11542326 11542189 ID=cds.MS.gene010443.t1;Parent=MS.gene010443.t1
chr5.4 exon 11542407 11543056 11542407 ID=MS.gene010443.t1.exon3;Parent=MS.gene010443.t1
chr5.4 CDS 11542407 11543056 11542407 ID=cds.MS.gene010443.t1;Parent=MS.gene010443.t1
chr5.4 exon 11544537 11545268 11544537 ID=MS.gene010443.t1.exon4;Parent=MS.gene010443.t1
chr5.4 CDS 11544537 11545268 11544537 ID=cds.MS.gene010443.t1;Parent=MS.gene010443.t1
Gene Sequence

>MS.gene010443

ATGTTTGATGAAGTTGGAGTTTTAGTTAATGAGATGCAAGTTGATGGAATATTACCTAGTCGAACGGTGAGTTTGTTATTGCAGCAGGTGAAGGATGAAGGATTTCTTAAGGATGTGGATGAATTGTTTGAAGGGATTTGCCCGGATGAAAGAATCAAGAGTGTAAGTTTTTCTATTGATAGCAGTGATGAGGATGAGAATGAGGACGAGGGTGGAAATGAGAATGAAGTTTCACAATGTGATCATGTAGATGGGATTCACTTAAAACCTTGGCTGGATCCGCGGTCTTTGGCTAGTGCGTTACAGAATTGGAGTCCTGATGAGGTATCAGCACTAGAGGGTGCAAAGTTCGTGTGGACAACTAGGTTGGTATGCAAGATACTTAGAAGTTTCAGGTCACCTGATACTGCGTGGAATTTCTTCTGCTGGGTTGCTGATCGACCTGGATTTACTCATAATATATCACAGTGCAAAGAATCATGACCCTTCTAGCACGACATGGACGTACCGAATTAGTTGATAGACTCATCTCGAAGATCAGAATAGAGGGAATGAGACTACCATTCAGTACCATAAGGTTAATCATTGACTTTTATGGGATTTCAAAAAATGCTGATGCTGCTCTGAAGGTTTTCAACAATGATCGAATACTTTGTGGGTCTATATCAAAAGTTAACTTGATGCTTCTGTATTCCTCTCTTTTAAGAACATTAACCAAGTGTGGAAGGGATTCCGATACCCTGGACATGCTTGATGAGATGATTTTAAATGGCATTTGCACAGATATCCAAACATTTTCAGGTCTAATGCAGTATTTTTCACAACTTGGAGACATTAAAACCGTGCAGAAACTCTTTTCAATGGTCAGACAGAGCGGTTTCGAGCCAGACGCGTATCTGTATAAGGTCCTGACAGAAGGTTATTGTAAGTCAACAAGAGCTGCACTGGCTTGGAGACTATTTGAAGATATGAAGAATTCTGGTTCCATACCTGATTCGGCCATCAAAGAGTTGCTAGTGAAGAGCCTCTGGAAAGAAGGTAGACGGAGAGAGGCCGCAGCTGTTGAAGAGAGCTGCGAGGAAGTAAATGTGGTCCTTCCACTTGCATTACCTGGTCATGTATGGACTGTCAGCTCTGAAGATCTCACAAAAGTCTATAACATTTATTCAAATAGTTTAGTTTCAAAGGGTGCCTAGAGTTATCCATTGGGCTATATGATAGAGGTGTACTTCTCCATGCATGAAATCTAAGCACAAAGTAACAAGCGGAAAGCAATAACCAAGTTCCTTAAACTGTGATTTATTTTGGGAGGGGTTGCCAAAAAGTTTTAAGGTGCAGTAATTTTTTGAGTTTTGAGAATGTATTATTGGCAGGACATGAGTTGATGCTCAGGAATTAGCTCTTGTTCTGATGGATATCGATCCTTCATTGTATTCACTTGGTCAAGTGCAAGAAATCATCTTTGCACAATCATATAATGAATGGACCTTTGGTTGATGTGTTGAGAGCTACGATCAGTCCTCTGCTGACTTCTGCAACTTGAGGGGAATCTAATGATTGTCTTATTGCAATTAACAATTCCTTCTAATTTTCTTACAAAAAGAGTTGGTTACAAACCTTCTAATTTTCTTACAAAAAGAAGAGTTGGTTACAAACCTTCTAATTTTCTTACAAAAAGAGTTGGTTACAAAATCTCATCTAATATATTGTTTTGATATATTGTTTTGAGTTCCTCTTTTTTTTTGTTGGTGTAAAAGGGTATATCTTTTCGCAACTCTGAATATTAATCCCTCGAATAGGGTTGGATAACATGAACGACAAAGTTGTTTCTTAAGAGTTTTAACACCGTTATATTCTTATGGCAGAATTCGAACTCGAACCCACTGGTTAAGCTAGAAGTAAGAAGAGACCCATGCTATGCCTAAAAAAAAGTGACCCATGTCATCTCATTCAATCGTTCTTGGCTTGTTTTCGGTTTAGATGAATATGAAGTACAAAAAGCTAGACTCAAGCAAAGGAGAGTAGGTAAGAAGGGCATCTTATTATTTTTAATATATTAGATGTCAAGCTAATGTGGCGATGTAAACACTGTTTATATCCGAAGTAGCTATGTACCAGAAATAGGGAACAAATAATATAAATTGTGAGTTTGTCAATGTGTTTTAATATATTGAAACCTATGTAACTGGAAGTGTCAACATTGTAAGCTGTTCATTGCCTGTATTATATTATAATTGCATATATATAATGGCATTAGCCTTTGTTAAACGTTAAACCATATCTTTTGCTTGTTTGCCCCTTAACATTTTGGTAAAGGCATCAAGTTAGATGGTCATTAGGTACCAGCCAGCTAGCTGACATCGTGTATGGACACCAAATTGGGTTATAATACCATATTATTCTTACCATCTTTATTATATTCTGCGCATGATTTCATACTTGTTTGGGATTTGCCAACAGTGTCTAAGATTTGAAAAAGAAAAATAGTAGAACTAATGAGAGAGACAGTGACTAAGATTTCATACTTTATTCTGCAAGTGCCCTTCAAAGAATCAATTGAACAAAGGAATACAGTATCTTATTACTTATGTGTGGCAGGATAACGACAATGTTACCCTTGTTAGTTCAAAGTTTGGTGCATCACCAGTTGAAATGTTAGCTGAAAACAATCATAACTTCACTGCTTCAACCGACTGTTCAGTTTTGATCCCTGTGACAAGTTTACCAAAACTTGATCAACCATCTTCAAATGGAAGAAAAAGCAGCAGTCAAAATCTGGCTCTTATAATTGGTATTAGCCTAGGAAGTGCTTTTTTCATTTTAGTTTTAACACCATCACTTGTGTACGTATATTGTCTCAAAATGAAGAGATTGAATAGAAGTACTTCATCGTCCGAGACTGCAGATAAGTTACTTTCAGGTGTTTCAGGTTATGTAAGCAAGCCAACAATGTATGAAATGGATGTGATCATGGAAGGTACAATGAATCTGAGTGAGAATTGTAAGATTGGTGAATCAGTTTACAAGGGTAATATAGATGGTAGAGTTTTAGCAGTGAAAAAAATCAAAAAAGATGCTTCTGAGGAGCTAAAAATTCTGCAGAAGGTAAACCATGGAAATATTGTGAAACTAATGGGTGTGTCTCCTGACAATGATGGAAACTGTTTTCTGGTCTATGAGTATGCTGAAAATGGATCACTTGAAGAGTGGTTGTTCTCGGAATCTTTGAAAACTTCAAACTCGGTGGTTTCGCTTACATGGTCTCAGAGAATAACCATAGCCATGGATGTTGCAATTGGTCTGCAAATGAACATACTTATCCAAGAATAA
Protein sequence

>MS.gene010443.t1

MFDEVGVLVNEMQVDGILPSRTVSLLLQQVKDEGFLKDVDELFEGICPDERIKSVNGIHLKPWLDPRSLASALQNWSPDEVSALEGAKFVWTTRLVCKILRMQRIMTLLARHGRTELVDRLISKIRIEGMRLPFSTIRLIIDFYGISKNADAALKVFNNDRILCGSISKVNLMLLYSSLLRTLTKCGRDSDTLDMLDEMILNGICTDIQTFSGLMQYFSQLGDIKTVQKLFSMVRQSGFEPDAYLYKVLTEGYCKSTRAALAWRLFEDMKNSGSIPDSAIKELLVKSLWKEGRRREAAAVEESCEEVNVVLPLALPGHDNDNVTLVSSKFGASPVEMLAENNHNFTASTDCSVLIPVTSLPKLDQPSSNGRKSSSQNLALIIGISLGSAFFILVLTPSLVYVYCLKMKRLNRSTSSSETADKLLSGVSGYVSKPTMYEMDVIMEGTMNLSENCKIGESVYKGNIDGRVLAVKKIKKDASEELKILQKVNHGNIVKLMGVSPDNDGNCFLVYEYAENGSLEEWLFSESLKTSNSVVSLTWSQRITIAMDVAIGLQMNILIQE