AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene010475

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010475.t1 MTR_5g019680 89.560 182 14 2 1 178 1 181 1.77e-107 306
MS.gene010475.t1 MTR_5g019650 81.250 192 19 3 1 176 1 191 1.39e-101 292
MS.gene010475.t1 MTR_4g107650 40.141 142 70 3 34 160 67 208 7.30e-23 93.6
MS.gene010475.t1 MTR_3g086790 46.087 115 55 1 54 168 60 167 1.45e-22 92.4
MS.gene010475.t1 MTR_6g007647 43.307 127 63 2 54 173 33 157 5.08e-22 90.1
MS.gene010475.t1 MTR_8g089895 56.250 80 35 0 45 124 45 124 5.92e-22 90.9
MS.gene010475.t1 MTR_6g007647 43.307 127 63 2 54 173 67 191 7.99e-22 90.1
MS.gene010475.t1 MTR_6g011610 50.505 99 49 0 45 143 87 185 2.19e-20 85.9
MS.gene010475.t1 MTR_5g038280 60.563 71 28 0 54 124 87 157 2.36e-20 86.7
MS.gene010475.t1 MTR_6g011610 50.505 99 49 0 45 143 68 166 2.65e-20 85.5
MS.gene010475.t1 MTR_8g468210 46.809 94 50 0 46 139 69 162 3.21e-20 85.9
MS.gene010475.t1 MTR_1g061660 45.545 101 55 0 54 154 59 159 3.24e-20 84.7
MS.gene010475.t1 MTR_8g469430 58.571 70 29 0 54 123 89 158 5.34e-20 85.1
MS.gene010475.t1 MTR_5g039000 44.737 114 58 1 46 159 69 177 5.85e-20 85.1
MS.gene010475.t1 MTR_3g092150 47.959 98 44 1 56 153 40 130 8.90e-20 84.0
MS.gene010475.t1 MTR_3g080100 43.396 106 60 0 48 153 14 119 6.66e-19 80.9
MS.gene010475.t1 MTR_7g103340 45.918 98 53 0 48 145 57 154 9.85e-19 80.9
MS.gene010475.t1 MTR_3g080100 44.660 103 57 0 51 153 56 158 1.24e-18 80.9
MS.gene010475.t1 MTR_8g026960 58.462 65 27 0 58 122 38 102 1.75e-17 77.8
MS.gene010475.t1 MTR_7g010020 47.778 90 47 0 54 143 80 169 2.05e-15 72.4
MS.gene010475.t1 MTR_4g084750 43.564 101 43 1 54 154 65 151 1.00e-13 66.6
MS.gene010475.t1 MTR_4g100550 43.564 101 43 1 54 154 120 206 3.86e-13 66.2
MS.gene010475.t1 MTR_8g006705 45.263 95 47 1 54 143 146 240 1.73e-12 64.7
MS.gene010475.t1 MTR_1g054285 38.938 113 55 1 42 154 142 240 1.89e-12 64.7
MS.gene010475.t1 MTR_2g061030 38.017 121 61 1 34 154 180 286 2.17e-12 64.7
MS.gene010475.t1 MTR_4g097600 45.263 95 47 1 54 143 127 221 3.19e-12 63.5
MS.gene010475.t1 MTR_1g017090 42.574 101 44 1 54 154 130 216 4.19e-12 63.5
MS.gene010475.t1 MTR_7g093430 40.566 106 49 1 49 154 155 246 1.34e-11 62.0
MS.gene010475.t1 MTR_5g014890 45.263 95 47 1 54 143 123 217 3.91e-11 60.5
MS.gene010475.t1 MTR_3g103590 43.836 73 41 0 52 124 143 215 8.78e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene010475.t1 AT4G36740 60.000 160 56 6 1 155 1 157 8.53e-56 176
MS.gene010475.t1 AT4G36740 61.589 151 52 5 7 155 10 156 3.23e-55 174
MS.gene010475.t1 AT2G18550 50.777 193 73 6 1 174 1 190 3.60e-55 174
MS.gene010475.t1 AT5G66700 53.659 164 63 3 5 155 9 172 1.33e-45 150
MS.gene010475.t1 AT3G01470 33.333 207 104 5 2 174 13 219 2.42e-24 96.7
MS.gene010475.t1 AT1G69780 38.961 154 80 3 2 143 23 174 1.75e-23 94.7
MS.gene010475.t1 AT2G22430 64.789 71 25 0 54 124 62 132 4.15e-22 91.3
MS.gene010475.t1 AT4G40060 63.380 71 26 0 54 124 59 129 5.31e-22 90.9
MS.gene010475.t1 AT5G65310 57.143 77 33 0 43 119 43 119 2.50e-21 89.0
MS.gene010475.t1 AT5G65310 57.143 77 33 0 43 119 61 137 2.76e-21 89.0
MS.gene010475.t1 AT5G53980 36.429 140 89 0 45 184 2 141 5.74e-21 85.1
MS.gene010475.t1 AT2G46680 48.000 100 51 1 48 146 26 125 4.62e-20 84.7
MS.gene010475.t1 AT5G03790 39.683 126 75 1 14 139 38 162 4.75e-20 84.3
MS.gene010475.t1 AT2G46680 45.370 108 52 1 48 155 26 126 5.40e-20 84.7
MS.gene010475.t1 AT5G03790 40.496 121 71 1 19 139 43 162 8.11e-20 82.8
MS.gene010475.t1 AT2G36610 45.370 108 51 2 46 145 63 170 4.25e-19 80.9
MS.gene010475.t1 AT1G26960 52.222 90 43 0 54 143 71 160 5.66e-19 82.0
MS.gene010475.t1 AT3G01220 51.852 81 38 1 45 124 77 157 5.09e-18 79.7
MS.gene010475.t1 AT3G61890 44.444 99 55 0 57 155 33 131 7.58e-18 78.6
MS.gene010475.t1 AT5G15150 58.209 67 28 0 54 120 115 181 1.02e-17 79.3
MS.gene010475.t1 AT1G27045 42.149 121 57 1 52 159 15 135 4.35e-17 75.5
MS.gene010475.t1 AT1G27045 42.149 121 57 1 52 159 23 143 4.96e-17 75.5
MS.gene010475.t1 AT1G27045 42.149 121 57 1 52 159 23 143 4.96e-17 75.5
MS.gene010475.t1 AT1G27045 42.149 121 57 1 52 159 66 186 8.01e-17 75.9
MS.gene010475.t1 AT1G27045 53.521 71 33 0 52 122 66 136 3.57e-16 74.3
MS.gene010475.t1 AT2G22800 44.211 95 48 1 54 143 113 207 2.26e-12 64.3
MS.gene010475.t1 AT2G01430 36.975 119 57 2 54 168 139 243 2.94e-12 63.9
MS.gene010475.t1 AT4G16780 41.905 105 51 2 44 143 124 223 4.91e-12 63.5
MS.gene010475.t1 AT5G47370 35.659 129 78 1 20 143 96 224 1.00e-11 62.4
MS.gene010475.t1 AT4G37790 43.158 95 49 1 54 143 126 220 2.13e-11 61.6
MS.gene010475.t1 AT4G17460 40.385 104 57 1 45 143 126 229 2.23e-11 61.6
MS.gene010475.t1 AT5G06710 36.667 120 62 1 35 154 171 276 4.21e-11 60.8

Find 32 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTGTTGAGAAATTATT+TGG 0.079303 5.2:-11135658 None:intergenic
TCCACCTAGCCCTTCGATTC+TGG 0.220585 5.2:-11135983 None:intergenic
TGATGAACAAATGAAAATTT+TGG 0.274227 5.2:+11135863 MS.gene010475:CDS
GAACCAAACTGCAACTTGTC+TGG 0.307826 5.2:-11135961 None:intergenic
GAAGTACTAGAGCTACTATT+TGG 0.364392 5.2:-11137070 None:intergenic
ATCCCAGACAAGTTGCAGTT+TGG 0.404850 5.2:+11135958 MS.gene010475:CDS
TTCTTACTACGCTTGCGTCT+TGG 0.426044 5.2:-11135783 None:intergenic
ACCTTGTTGAGAAATTATTT+GGG 0.435398 5.2:-11135657 None:intergenic
AGAGGTATACCTTGGTTTCA+AGG 0.440743 5.2:-11136079 None:intergenic
ATTTGGGTGTATTCACAAGC+TGG 0.458844 5.2:-11135641 None:intergenic
ACTGAGACATAAGCATCATT+TGG 0.463969 5.2:-11135613 None:intergenic
TCAAATTCATCAAGTAATTG+TGG 0.464011 5.2:-11137112 None:intergenic
GATAGACTTGCAATGGAGCT+TGG 0.475779 5.2:+11135930 MS.gene010475:CDS
AAGGAGACACTTCTCAAGAA+TGG 0.492858 5.2:-11136060 None:intergenic
CAAATTCATCAAGTAATTGT+GGG 0.494706 5.2:-11137111 None:intergenic
TAAACTTGAGTCAGAGAAGA+AGG 0.499532 5.2:+11135908 MS.gene010475:CDS
AACCAAACTGCAACTTGTCT+GGG 0.501567 5.2:-11135960 None:intergenic
CAGTTTGGTTCCAGAATCGA+AGG 0.504708 5.2:+11135973 MS.gene010475:CDS
TTTGGGTGTATTCACAAGCT+GGG 0.519334 5.2:-11135640 None:intergenic
GGTTCCAGAATCGAAGGGCT+AGG 0.526167 5.2:+11135979 MS.gene010475:CDS
GCAGCTTTCTGAGGCAGAGA+AGG 0.540038 5.2:+11137027 MS.gene010475:CDS
GAAGAAGGATAGACTTGCAA+TGG 0.545678 5.2:+11135923 MS.gene010475:CDS
TACTTCATCAATTTCAGTAG+TGG 0.546036 5.2:+11137087 MS.gene010475:CDS
AGCTACTATTTGGCTCTCGA+AGG 0.571565 5.2:-11137060 None:intergenic
GCTAACAGAGCAGCTTTCTG+AGG 0.593252 5.2:+11137018 MS.gene010475:CDS
CGTAGTAAGAAGAACAAAGG+TGG 0.606299 5.2:+11135795 MS.gene010475:CDS
GAGACACTTCTCAAGAATGG+TGG 0.632257 5.2:-11136057 None:intergenic
GAAGTGTCTCCTTGAAACCA+AGG 0.638232 5.2:+11136070 MS.gene010475:CDS
ACCCAAATAATTTCTCAACA+AGG 0.645027 5.2:+11135656 MS.gene010475:CDS
TCCAGAATCGAAGGGCTAGG+TGG 0.655135 5.2:+11135982 MS.gene010475:CDS
AAGCGTAGTAAGAAGAACAA+AGG 0.668285 5.2:+11135792 MS.gene010475:CDS
AGTTTGGTTCCAGAATCGAA+GGG 0.690392 5.2:+11135974 MS.gene010475:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTAATTGAACATATA+AGG - chr5.2:11136985-11137004 None:intergenic 10.0%
!! AATATATAAAAACAAAAACA+AGG + chr5.2:11136692-11136711 MS.gene010475:intron 10.0%
!! ATAAAACAAACAAAAAAATA+CGG - chr5.2:11138170-11138189 None:intergenic 10.0%
!! ATAATAAATTAAATAAGAAG+AGG - chr5.2:11136110-11136129 None:intergenic 10.0%
!! TTATTAAAAATTAAAATCAG+TGG + chr5.2:11136183-11136202 MS.gene010475:intron 10.0%
!!! AAAAATTCTAATTTTTTTTC+AGG + chr5.2:11137446-11137465 MS.gene010475:intron 10.0%
!!! AATTTAGAATGAAATATTAA+AGG - chr5.2:11138953-11138972 None:intergenic 10.0%
!!! ATAACTAATCATTTTTTTTA+GGG - chr5.2:11138444-11138463 None:intergenic 10.0%
!!! ATTTTTGTTATGTTTTTTTA+GGG + chr5.2:11135745-11135764 MS.gene010475:intron 10.0%
!!! TATAATATTTTGAATTTTTC+TGG - chr5.2:11137320-11137339 None:intergenic 10.0%
!!! TATTTTTGTTATGTTTTTTT+AGG + chr5.2:11135744-11135763 MS.gene010475:intron 10.0%
!! AAAAATCATAATTATGTCAA+TGG - chr5.2:11137910-11137929 None:intergenic 15.0%
!! AAAATGAAGAAAATATTGTA+CGG - chr5.2:11136784-11136803 None:intergenic 15.0%
!! AACAATTAATTTACATATTG+AGG - chr5.2:11137799-11137818 None:intergenic 15.0%
!! ACAATTAATTTACATATTGA+GGG - chr5.2:11137798-11137817 None:intergenic 15.0%
!! GAAAAATTGACATATTTATA+GGG + chr5.2:11137611-11137630 MS.gene010475:intron 15.0%
!! GAAGTTAATCATATATATAA+GGG + chr5.2:11137133-11137152 MS.gene010475:intron 15.0%
!! TATGTAAATTAATTGTTCAT+AGG + chr5.2:11137802-11137821 MS.gene010475:intron 15.0%
!! TGAAGTTAATCATATATATA+AGG + chr5.2:11137132-11137151 MS.gene010475:intron 15.0%
!!! AAAATTTTGGAAGAAAATTT+TGG + chr5.2:11135876-11135895 MS.gene010475:CDS 15.0%
!!! ACATAAATTAAGTTGAAAAT+AGG + chr5.2:11137519-11137538 MS.gene010475:intron 15.0%
!!! ACTTTATCATTTTTCATTTA+AGG - chr5.2:11136508-11136527 None:intergenic 15.0%
!!! CATAAATTAAGTTGAAAATA+GGG + chr5.2:11137520-11137539 MS.gene010475:intron 15.0%
!!! CTTTATCATTTTTCATTTAA+GGG - chr5.2:11136507-11136526 None:intergenic 15.0%
!!! GATAACTAATCATTTTTTTT+AGG - chr5.2:11138445-11138464 None:intergenic 15.0%
!!! TAACTAATCATTTTTTTTAG+GGG - chr5.2:11138443-11138462 None:intergenic 15.0%
!!! TAGTGATTGAAAATTTTATA+GGG + chr5.2:11137551-11137570 MS.gene010475:intron 15.0%
!!! TGAAGATTGAAAATTTTATA+GGG - chr5.2:11137236-11137255 None:intergenic 15.0%
!!! TTTATCATTTTTCATTTAAG+GGG - chr5.2:11136506-11136525 None:intergenic 15.0%
!!! TTTTTATTTTGACCTATAAA+TGG + chr5.2:11137925-11137944 MS.gene010475:intron 15.0%
!!! TTTTTGTTATGTTTTTTTAG+GGG + chr5.2:11135746-11135765 MS.gene010475:intron 15.0%
!!! TTTTTTGTTTGTTTTATCTT+TGG + chr5.2:11138173-11138192 MS.gene010475:intron 15.0%
!! AAAAAAACACTACCATTTAT+AGG - chr5.2:11137940-11137959 None:intergenic 20.0%
!! AACACATCATCATTATATAT+AGG - chr5.2:11137655-11137674 None:intergenic 20.0%
!! AATAATTCTTATATGTACTG+AGG - chr5.2:11138351-11138370 None:intergenic 20.0%
!! ACACATCATCATTATATATA+GGG - chr5.2:11137654-11137673 None:intergenic 20.0%
!! ATTAAGTCAAACTATTCATT+TGG + chr5.2:11138093-11138112 MS.gene010475:intron 20.0%
!! ATTGAACATATAAGGTAATT+AGG - chr5.2:11136977-11136996 None:intergenic 20.0%
!! CACTAGTAATATAATATTGT+TGG - chr5.2:11136258-11136277 None:intergenic 20.0%
!! CGAAAAATTGACATATTTAT+AGG + chr5.2:11137610-11137629 MS.gene010475:intron 20.0%
!! GAAAAGTTGACATATTTATA+GGG - chr5.2:11137176-11137195 None:intergenic 20.0%
!! GAAATAATATAGTTGTTAGT+TGG + chr5.2:11138626-11138645 MS.gene010475:intron 20.0%
!! TCTAAGCAAAGATTATTAAA+AGG - chr5.2:11136933-11136952 None:intergenic 20.0%
!!! AAATTTTCAATCACTACTTT+TGG - chr5.2:11137547-11137566 None:intergenic 20.0%
!!! AAATTTTCAATCTTCACTTT+TGG + chr5.2:11137240-11137259 MS.gene010475:intron 20.0%
!!! AAGTTGAAAGAAAATTTTAG+AGG - chr5.2:11137208-11137227 None:intergenic 20.0%
!!! AATTTATCATCTTTTAGAGA+GGG - chr5.2:11138414-11138433 None:intergenic 20.0%
!!! AGTTGAAAGAAAATTTTAGA+GGG - chr5.2:11137207-11137226 None:intergenic 20.0%
!!! ATAAGGGTTAAATATGTTTT+TGG + chr5.2:11137149-11137168 MS.gene010475:intron 20.0%
!!! ATATGAGTTTACTTTAAAAG+AGG - chr5.2:11137268-11137287 None:intergenic 20.0%
!!! GTACAATATTTTCTTCATTT+TGG + chr5.2:11136783-11136802 MS.gene010475:intron 20.0%
!!! GTAGTGATTGAAAATTTTAT+AGG + chr5.2:11137550-11137569 MS.gene010475:intron 20.0%
!!! GTGAAGATTGAAAATTTTAT+AGG - chr5.2:11137237-11137256 None:intergenic 20.0%
!!! TAAAATTTTCCTTCAACTTT+TGG - chr5.2:11137578-11137597 None:intergenic 20.0%
!!! TAATTTATCATCTTTTAGAG+AGG - chr5.2:11138415-11138434 None:intergenic 20.0%
!!! TATAGGGTTAAATATGTTTT+TGG - chr5.2:11137638-11137657 None:intergenic 20.0%
!!! TATGAGTTTACTTTAAAAGA+GGG - chr5.2:11137267-11137286 None:intergenic 20.0%
!!! TATTTGTTTCTCCTTAATTA+TGG + chr5.2:11138747-11138766 MS.gene010475:intron 20.0%
!!! TGATGAACAAATGAAAATTT+TGG + chr5.2:11135863-11135882 MS.gene010475:CDS 20.0%
!!! TTCACATGGATTATTTTTAA+GGG + chr5.2:11138035-11138054 MS.gene010475:intron 20.0%
!!! TTTCACATGGATTATTTTTA+AGG + chr5.2:11138034-11138053 MS.gene010475:intron 20.0%
! AACCTTGTTGAGAAATTATT+TGG - chr5.2:11135661-11135680 None:intergenic 25.0%
! AACTATTCATTTGGATAAGT+TGG + chr5.2:11138102-11138121 MS.gene010475:intron 25.0%
! ACCAAATATTGAACAAAAAG+AGG + chr5.2:11135832-11135851 MS.gene010475:CDS 25.0%
! ACCTTGTTGAGAAATTATTT+GGG - chr5.2:11135660-11135679 None:intergenic 25.0%
! ACTATTCATTTGGATAAGTT+GGG + chr5.2:11138103-11138122 MS.gene010475:intron 25.0%
! AGAAAATATTGTACGGAATT+AGG - chr5.2:11136777-11136796 None:intergenic 25.0%
! AGCATTTGTAGATAATGTAA+AGG + chr5.2:11137768-11137787 MS.gene010475:intron 25.0%
! ATTCTCTCTTTCAACATTTA+GGG - chr5.2:11136432-11136451 None:intergenic 25.0%
! CAAATTCATCAAGTAATTGT+GGG - chr5.2:11137114-11137133 None:intergenic 25.0%
! CGAAAAGTTGACATATTTAT+AGG - chr5.2:11137177-11137196 None:intergenic 25.0%
! GAGAACTTTAATAATGTGAA+CGG - chr5.2:11136596-11136615 None:intergenic 25.0%
! GTGTGTTTATTATATGACTA+TGG + chr5.2:11137868-11137887 MS.gene010475:intron 25.0%
! TATAAAGATTAATCCATTCC+TGG - chr5.2:11137711-11137730 None:intergenic 25.0%
! TATTCTCTCTTTCAACATTT+AGG - chr5.2:11136433-11136452 None:intergenic 25.0%
! TCAAATTCATCAAGTAATTG+TGG - chr5.2:11137115-11137134 None:intergenic 25.0%
! TCTTTATCAATTACAGCTAT+AGG - chr5.2:11138570-11138589 None:intergenic 25.0%
!! AACGTAACTACGTTTTTAAT+TGG + chr5.2:11138516-11138535 MS.gene010475:intron 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG + chr5.2:11137579-11137598 MS.gene010475:intron 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG + chr5.2:11137580-11137599 MS.gene010475:intron 25.0%
!! TATTATATGACTATGGATTG+TGG + chr5.2:11137875-11137894 MS.gene010475:intron 25.0%
!! TCCTCTTTTTGTTCAATATT+TGG - chr5.2:11135836-11135855 None:intergenic 25.0%
!!! ATTTGTTTTTCTCAAATGCT+AGG + chr5.2:11138368-11138387 MS.gene010475:intron 25.0%
!!! TATAGTTGTTAGTTGGTAAT+AGG + chr5.2:11138633-11138652 MS.gene010475:intron 25.0%
!!! TCACATGGATTATTTTTAAG+GGG + chr5.2:11138036-11138055 MS.gene010475:intron 25.0%
!!! TTGTTTTTAACGTTTGAACA+TGG - chr5.2:11138773-11138792 None:intergenic 25.0%
AACACTAGTAACGTCAATTT+AGG + chr5.2:11136302-11136321 MS.gene010475:intron 30.0%
ACACTAGTAACGTCAATTTA+GGG + chr5.2:11136303-11136322 MS.gene010475:intron 30.0%
ACATAACATGAATGATCATG+TGG - chr5.2:11136628-11136647 None:intergenic 30.0%
ACCCAAATAATTTCTCAACA+AGG + chr5.2:11135656-11135675 MS.gene010475:CDS 30.0%
ATAGTCTTTGCAGTTACATA+CGG - chr5.2:11138853-11138872 None:intergenic 30.0%
ATTGTTGGAGAAATGATGAT+TGG - chr5.2:11136243-11136262 None:intergenic 30.0%
CAAAAAGCGATAAAAGTTGA+GGG - chr5.2:11138812-11138831 None:intergenic 30.0%
GCCATATGAGATTAAATAAG+CGG + chr5.2:11138883-11138902 MS.gene010475:intron 30.0%
TACTTCATCAATTTCAGTAG+TGG + chr5.2:11137087-11137106 MS.gene010475:CDS 30.0%
TCTACTTCATGATAACAAGT+TGG - chr5.2:11136822-11136841 None:intergenic 30.0%
TGATAGCATGATAAAAGTTG+AGG - chr5.2:11138918-11138937 None:intergenic 30.0%
TTCCACGTCAACTATTTAAT+TGG - chr5.2:11138593-11138612 None:intergenic 30.0%
TTCTCTCTTTCAACATTTAG+GGG - chr5.2:11136431-11136450 None:intergenic 30.0%
TTTGAACATGGCCATAATTA+AGG - chr5.2:11138761-11138780 None:intergenic 30.0%
!! AGTGTTGTTTGTTGTCTTTA+AGG - chr5.2:11136736-11136755 None:intergenic 30.0%
!!! CGTTTTTAATTGGTCTTTTC+CGG + chr5.2:11138526-11138545 MS.gene010475:intron 30.0%
AAATAAGAAGAGGAAGCAAG+AGG - chr5.2:11136100-11136119 None:intergenic 35.0%
AAGCGTAGTAAGAAGAACAA+AGG + chr5.2:11135792-11135811 MS.gene010475:CDS 35.0%
ACGTATGCAAATTTAGCGTA+AGG - chr5.2:11138262-11138281 None:intergenic 35.0%
ACTGAGACATAAGCATCATT+TGG - chr5.2:11135616-11135635 None:intergenic 35.0%
AGATTGAATTCTAACCAAGC+GGG + chr5.2:11138141-11138160 MS.gene010475:intron 35.0%
ATAGCACATTGAAGATGAGA+TGG - chr5.2:11138013-11138032 None:intergenic 35.0%
CCAAAAAGCGATAAAAGTTG+AGG - chr5.2:11138813-11138832 None:intergenic 35.0%
CTTTAATAATGTGAACGGCT+TGG - chr5.2:11136591-11136610 None:intergenic 35.0%
GACCAATTAAATAGTTGACG+TGG + chr5.2:11138588-11138607 MS.gene010475:intron 35.0%
GAGATTGAATTCTAACCAAG+CGG + chr5.2:11138140-11138159 MS.gene010475:intron 35.0%
GATGCGACATATTACTTCAA+CGG + chr5.2:11137685-11137704 MS.gene010475:intron 35.0%
GCCGCTTATTTAATCTCATA+TGG - chr5.2:11138887-11138906 None:intergenic 35.0%
TAAACTTGAGTCAGAGAAGA+AGG + chr5.2:11135908-11135927 MS.gene010475:CDS 35.0%
TCAATGTGCTATCTTTCACA+TGG + chr5.2:11138021-11138040 MS.gene010475:intron 35.0%
TTATAGGGACCAAAAGTTGA+AGG + chr5.2:11137566-11137585 MS.gene010475:intron 35.0%
TTGAAAGAGAGAATAAGCAC+TGG + chr5.2:11136438-11136457 MS.gene010475:intron 35.0%
TTGAAGTAATATGTCGCATC+CGG - chr5.2:11137685-11137704 None:intergenic 35.0%
! ACATTGATTAGAGGGTAATG+GGG - chr5.2:11136875-11136894 None:intergenic 35.0%
! GAAGTACTAGAGCTACTATT+TGG - chr5.2:11137073-11137092 None:intergenic 35.0%
! GACATTGATTAGAGGGTAAT+GGG - chr5.2:11136876-11136895 None:intergenic 35.0%
! TGGATAAGTTGGGCTAAATA+TGG + chr5.2:11138113-11138132 MS.gene010475:intron 35.0%
! TTCATTTTGGACCTTTCTCA+AGG + chr5.2:11136796-11136815 MS.gene010475:intron 35.0%
!! CCTCAACTTTTATCGCTTTT+TGG + chr5.2:11138810-11138829 MS.gene010475:intron 35.0%
!! TGATGATGTGTTTTTCATGC+CGG + chr5.2:11137663-11137682 MS.gene010475:intron 35.0%
!!! GACGTGGAATTTTTTTTGTG+TGG + chr5.2:11138604-11138623 MS.gene010475:intron 35.0%
AACCAAACTGCAACTTGTCT+GGG - chr5.2:11135963-11135982 None:intergenic 40.0%
AAGGAGACACTTCTCAAGAA+TGG - chr5.2:11136063-11136082 None:intergenic 40.0%
ATATGCACGTTCATATGTGG+TGG - chr5.2:11136543-11136562 None:intergenic 40.0%
ATGCTACCATGTTTCTTCTC+TGG + chr5.2:11136331-11136350 MS.gene010475:intron 40.0%
ATTTGGGTGTATTCACAAGC+TGG - chr5.2:11135644-11135663 None:intergenic 40.0%
CGTAGTAAGAAGAACAAAGG+TGG + chr5.2:11135795-11135814 MS.gene010475:CDS 40.0%
CTCATATGCACGTTCATATG+TGG - chr5.2:11136546-11136565 None:intergenic 40.0%
CTTATATGTACTGAGGTCTG+AGG - chr5.2:11138344-11138363 None:intergenic 40.0%
GAAGAAGGATAGACTTGCAA+TGG + chr5.2:11135923-11135942 MS.gene010475:CDS 40.0%
GAAGATGAGATGGAAACACA+CGG - chr5.2:11138003-11138022 None:intergenic 40.0%
TAATGAAAGAGACATGCACG+TGG - chr5.2:11136904-11136923 None:intergenic 40.0%
TATGCACGTTCATATGTGGT+GGG - chr5.2:11136542-11136561 None:intergenic 40.0%
TTTGGGTGTATTCACAAGCT+GGG - chr5.2:11135643-11135662 None:intergenic 40.0%
! AGAGGTATACCTTGGTTTCA+AGG - chr5.2:11136082-11136101 None:intergenic 40.0%
! AGTTTGGTTCCAGAATCGAA+GGG + chr5.2:11135974-11135993 MS.gene010475:CDS 40.0%
! GGACATTGATTAGAGGGTAA+TGG - chr5.2:11136877-11136896 None:intergenic 40.0%
! GGTGTAGGACATTGATTAGA+GGG - chr5.2:11136883-11136902 None:intergenic 40.0%
! TGGTGTAGGACATTGATTAG+AGG - chr5.2:11136884-11136903 None:intergenic 40.0%
!! TAACAAGTTGGCCTTGAGAA+AGG - chr5.2:11136810-11136829 None:intergenic 40.0%
AAAAAATACGGTGACCCGCT+TGG - chr5.2:11138158-11138177 None:intergenic 45.0%
AGACCACCAGAGAAGAAACA+TGG - chr5.2:11136340-11136359 None:intergenic 45.0%
ATCCCAGACAAGTTGCAGTT+TGG + chr5.2:11135958-11135977 MS.gene010475:CDS 45.0%
ATGAACGTGCATATGAGCTC+TGG + chr5.2:11136549-11136568 MS.gene010475:intron 45.0%
ATTACTTCAACGGCCAGGAA+TGG + chr5.2:11137695-11137714 MS.gene010475:intron 45.0%
CTACCATGTTTCTTCTCTGG+TGG + chr5.2:11136334-11136353 MS.gene010475:intron 45.0%
GAACCAAACTGCAACTTGTC+TGG - chr5.2:11135964-11135983 None:intergenic 45.0%
GACATATTACTTCAACGGCC+AGG + chr5.2:11137690-11137709 MS.gene010475:intron 45.0%
GACTAAAAGCAAGACTGCAC+CGG - chr5.2:11138548-11138567 None:intergenic 45.0%
GAGACACTTCTCAAGAATGG+TGG - chr5.2:11136060-11136079 None:intergenic 45.0%
GATAGACTTGCAATGGAGCT+TGG + chr5.2:11135930-11135949 MS.gene010475:CDS 45.0%
TGAACGTGCATATGAGCTCT+GGG + chr5.2:11136550-11136569 MS.gene010475:intron 45.0%
TTCTTACTACGCTTGCGTCT+TGG - chr5.2:11135786-11135805 None:intergenic 45.0%
TTGCTTTGTCCTGAAGAACC+CGG - chr5.2:11136471-11136490 None:intergenic 45.0%
! AGGAAGCAAGAGGTATACCT+TGG - chr5.2:11136090-11136109 None:intergenic 45.0%
! CAGTTTGGTTCCAGAATCGA+AGG + chr5.2:11135973-11135992 MS.gene010475:CDS 45.0%
! GAAGTGTCTCCTTGAAACCA+AGG + chr5.2:11136070-11136089 MS.gene010475:CDS 45.0%
!! AATAAGCACTGGCATGCAAC+CGG + chr5.2:11136449-11136468 MS.gene010475:intron 45.0%
!! AGCTACTATTTGGCTCTCGA+AGG - chr5.2:11137063-11137082 None:intergenic 45.0%
!! AGGGTAATGGGGTTTCACTT+GGG - chr5.2:11136864-11136883 None:intergenic 45.0%
!!! AAAAAATTCTAAATTTTTTT+TGG - chr5.2:11137352-11137371 None:intergenic 5.0%
!!! ATGAATTTTTATATTTTTTA+CGG + chr5.2:11137391-11137410 MS.gene010475:intron 5.0%
AAGAGACATGCACGTGGTGT+AGG - chr5.2:11136898-11136917 None:intergenic 50.0%
GCTAACAGAGCAGCTTTCTG+AGG + chr5.2:11137018-11137037 MS.gene010475:CDS 50.0%
! ATGTACTGAGGTCTGAGGCT+AGG - chr5.2:11138339-11138358 None:intergenic 50.0%
!! ATAAGCACTGGCATGCAACC+GGG + chr5.2:11136450-11136469 MS.gene010475:intron 50.0%
!! GAGGGTAATGGGGTTTCACT+TGG - chr5.2:11136865-11136884 None:intergenic 50.0%
GGTTCCAGAATCGAAGGGCT+AGG + chr5.2:11135979-11135998 MS.gene010475:CDS 55.0%
TCCACCTAGCCCTTCGATTC+TGG - chr5.2:11135986-11136005 None:intergenic 55.0%
TCCAGAATCGAAGGGCTAGG+TGG + chr5.2:11135982-11136001 MS.gene010475:CDS 55.0%
!! GCAGCTTTCTGAGGCAGAGA+AGG + chr5.2:11137027-11137046 MS.gene010475:CDS 55.0%
GGCATGCAACCGGGTTCTTC+AGG + chr5.2:11136459-11136478 MS.gene010475:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 11135599 11139040 11135599 ID=MS.gene010475
chr5.2 mRNA 11135599 11139040 11135599 ID=MS.gene010475.t1;Parent=MS.gene010475
chr5.2 exon 11135599 11135677 11135599 ID=MS.gene010475.t1.exon1;Parent=MS.gene010475.t1
chr5.2 CDS 11135599 11135677 11135599 ID=cds.MS.gene010475.t1;Parent=MS.gene010475.t1
chr5.2 exon 11135766 11136091 11135766 ID=MS.gene010475.t1.exon2;Parent=MS.gene010475.t1
chr5.2 CDS 11135766 11136091 11135766 ID=cds.MS.gene010475.t1;Parent=MS.gene010475.t1
chr5.2 exon 11137010 11137135 11137010 ID=MS.gene010475.t1.exon3;Parent=MS.gene010475.t1
chr5.2 CDS 11137010 11137135 11137010 ID=cds.MS.gene010475.t1;Parent=MS.gene010475.t1
chr5.2 exon 11139008 11139040 11139008 ID=MS.gene010475.t1.exon4;Parent=MS.gene010475.t1
chr5.2 CDS 11139008 11139040 11139008 ID=cds.MS.gene010475.t1;Parent=MS.gene010475.t1
Gene Sequence

>MS.gene010475

ATGAATCATCAAGACCAAATGATGCTTATGTCTCAGTTATTCCCAGCTTGTGAATACACCCAAATAATTTCTCAACAAGGTTAGTATCATATTAAATTAATACATAACATTTTATTGTATCATAAAAATTTCTATGAAGTTATAATATTTTTGTTATGTTTTTTTAGGGGAGAAGAAGCTAAAGCCAAGACGCAAGCGTAGTAAGAAGAACAAAGGTGGAGAAAACAGTGCTAACCAAATATTGAACAAAAAGAGGAAATTGAGTGATGAACAAATGAAAATTTTGGAAGAAAATTTTGGAAATGAACATAAACTTGAGTCAGAGAAGAAGGATAGACTTGCAATGGAGCTTGGTTTAGATCCCAGACAAGTTGCAGTTTGGTTCCAGAATCGAAGGGCTAGGTGGAAGAACAAGAAACTTGAAGAAGAGTACTTAACTCTTAAAAAAATTCATGAATCCACCATTCTTGAGAAGTGTCTCCTTGAAACCAAGGTATACCTCTTGCTTCCTCTTCTTATTTAATTTATTATTGCAACTAGAAACAATTTTATTAAACTATGTTAACATGTTTTTTAATTAACTATTATTAAAAATTAAAATCAGTGGATTGAATTATAACTTGACATCAAAATTTTAATTTCCAATCATCATTTCTCCAACAATATTATATTACTAGTGTTTTGTTTTTATTTGTCTCAACGCAACACTAGTAACGTCAATTTAGGGCAAGCATGCTACCATGTTTCTTCTCTGGTGGTCTTGATATTTTGTTTATTCACTATATTGTTGTTATAACTTTGTTTAAAAAAGTTGTTTGCATAGTTTAATCCCCTAAATGTTGAAAGAGAGAATAAGCACTGGCATGCAACCGGGTTCTTCAGGACAAAGCAAAAAGAAAAGAAACCCCTTAAATGAAAAATGATAAAGTAGAAACTTAGACCCACCACATATGAACGTGCATATGAGCTCTGGGCTCGAAGAAATTAATCCAAGCCGTTCACATTATTAAAGTTCTCACTTCTCAGCCACATGATCATTCATGTTATGTAGATTTTCTTTAAAAAAATGCTAATGATACATAAAATTAAAAGAAATATATAAAAACAAAAACAAGGAACAGCAAAAAAATATCACCTTAAAGACAACAAACAACACTGTTTCTTTCAATTTGTTTCCTAATTCCGTACAATATTTTCTTCATTTTGGACCTTTCTCAAGGCCAACTTGTTATCATGAAGTAGAAACGTGACATACATATGTTCCCAAGTGAAACCCCATTACCCTCTAATCAATGTCCTACACCACGTGCATGTCTCTTTCATTATCTTAACCTTTTAATAATCTTTGCTTAGATTAGATCTCATCATCTTAACTCCTAATTACCTTATATGTTCAATTAATATTTTGTAGATGTTGAAGCTAACAGAGCAGCTTTCTGAGGCAGAGAAGGAAATTCAACGCCTTCGAGAGCCAAATAGTAGCTCTAGTACTTCATCAATTTCAGTAGTGGACCCACAATTACTTGATGAATTTGAAGTTAATCATATATATAAGGGTTAAATATGTTTTTGGTCCCTATAAATATGTCAACTTTTCGTTTTAGTCCCTCTAAAATTTTCTTTCAACTTTTAGTCCCTATAAAATTTTCAATCTTCACTTTTGGTCCCTCTTTTAAAGTAAACTCATATGTAGAATTCATATTTTTTAATAAAATTTTCCAGAAAAATTCAAAATATTATAAGAATCACTCCAAAAAAAATTTAGAATTTTTTAACAAAACATGAATTTAATATGAATTTTTATATTTTTTACGGTTAAAAATTCATATTTAATTTGTGTTTTGTTAAAAAATTCTAATTTTTTTTCAGGAAATTGTTTTAGAATATTTTACACATTTCTGCAAAATTTTATTAAAAAATACATAAATTAAGTTGAAAATAGGGACCAAAAGTAGTGATTGAAAATTTTATAGGGACCAAAAGTTGAAGGAAAATTTTAGAGGGACTAAAACGAAAAATTGACATATTTATAGGGACCAAAAACATATTTAACCCTATATATAATGATGATGTGTTTTTCATGCCGGATGCGACATATTACTTCAACGGCCAGGAATGGATTAATCTTTATATCTAAAAAATAGTAAAACAATAAGAACTAATTAACTTAGCATTTGTAGATAATGTAAAGGATATCCCTCAATATGTAAATTAATTGTTCATAGGTACTTAATTAGTGTGTTTATTATATAACGTCAAAAAAAAAAAAGTGTGTTTATTATATGACTATGGATTGTGGAATATTTATCCATTGACATAATTATGATTTTTATTTTGACCTATAAATGGTAGTGTTTTTTTGTCGTTGCACTAACTATGCTTTTGTCACATATATAATGTGCCGTGTGTTTCCATCTCATCTTCAATGTGCTATCTTTCACATGGATTATTTTTAAGGGGAAGCAATGCATATGCATACAAATTGAATGCTAAGATTAAGTCAAACTATTCATTTGGATAAGTTGGGCTAAATATGGCAATGAGATTGAATTCTAACCAAGCGGGTCACCGTATTTTTTTGTTTGTTTTATCTTTGGAAATATATGCATATTCTTCTGAGCTATTGTTTGTGAATGTATTTTAAAAATAGTAGAATATTTCCTTACGCTAAATTTGCATACGTTTATGTCTATATGCATGAACACGTTTAAGTTATCATGTGACTGTAATATTCATTCCTAGCCTCAGACCTCAGTACATATAAGAATTATTTGTTTTTCTCAAATGCTAGGTAGTGTCACTAATTATCTTTCCCTCTCTAAAAGATGATAAATTATCTTTCCCCTAAAAAAAATGATTAGTTATCTTTTTTTTATAAGATAATATGCTTAACTATCTGAACATGTAATCTAATATAAACGTAACTACGTTTTTAATTGGTCTTTTCCGGTGCAGTCTTGCTTTTAGTCCTATAGCTGTAATTGATAAAGACCAATTAAATAGTTGACGTGGAATTTTTTTTGTGTGGAAATAATATAGTTGTTAGTTGGTAATAGGAGTATTTTAAAAAACTATTCGTTAACGATATGATGATGAGAAAGAGAAATTTAAAAAATTGCAGAGAGATATCATAGATAAAAGCTTTAATTATTTGTTTCTCCTTAATTATGGCCATGTTCAAACGTTAAAAACAAACTACGAATTTTCTCTCCCTCAACTTTTATCGCTTTTTGGTGTAAATTGAGAATAATCCGTATGTAACTGCAAAGACTATAATCACTTGAGCCATATGAGATTAAATAAGCGGCAAACTCTTCCTCAACTTTTATCATGCTATCACTTAATTTATCTCCTTTAATATTTCATTCTAAATTTTCATGAAAATTATGATCTTCTATTATCTTTTTAGATGATATTAGTGTTGTACGTAGAAAAAGCATGA
Protein sequence

>MS.gene010475.t1

MNHQDQMMLMSQLFPACEYTQIISQQGEKKLKPRRKRSKKNKGGENSANQILNKKRKLSDEQMKILEENFGNEHKLESEKKDRLAMELGLDPRQVAVWFQNRRARWKNKKLEEEYLTLKKIHESTILEKCLLETKMLKLTEQLSEAEKEIQRLREPNSSSSTSSISVVDPQLLDEFEMILVLYVEKA